SitesBLAST
Comparing WP_012505142.1 NCBI__GCF_000020625.1:WP_012505142.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
1iyeA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
52% identity, 98% coverage: 3:300/303 of query aligns to 1:300/304 of 1iyeA
- active site: F33 (= F35), G35 (= G37), K156 (= K157), A157 (= A158), E190 (= E191), L214 (= L215)
- binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: R56 (= R58), Y92 (= Y94), Y126 (= Y127), K156 (= K157), Y161 (= Y162), E190 (= E191), G193 (= G194), E194 (= E195), N195 (= N196), L214 (= L215), G216 (= G217), I217 (= I218), T218 (= T219), G253 (= G254), T254 (= T255), A255 (= A256)
1iydA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
52% identity, 98% coverage: 3:300/303 of query aligns to 1:300/304 of 1iydA
- active site: F33 (= F35), G35 (= G37), K156 (= K157), A157 (= A158), E190 (= E191), L214 (= L215)
- binding glutaric acid: Y92 (= Y94), Y126 (= Y127), A255 (= A256)
- binding pyridoxal-5'-phosphate: R56 (= R58), K156 (= K157), Y161 (= Y162), E190 (= E191), G193 (= G194), E194 (= E195), L214 (= L215), G216 (= G217), I217 (= I218), T218 (= T219), T254 (= T255)
1i1mA Crystal structure of escherichia coli branched-chain amino acid aminotransferase. (see paper)
52% identity, 98% coverage: 3:300/303 of query aligns to 1:300/304 of 1i1mA
- active site: K156 (= K157)
- binding 4-methyl valeric acid: Y92 (= Y94), K156 (= K157), T254 (= T255), A255 (= A256)
- binding pyridoxal-5'-phosphate: R56 (= R58), K156 (= K157), Y161 (= Y162), E190 (= E191), G193 (= G194), E194 (= E195), L214 (= L215), G216 (= G217), I217 (= I218), T218 (= T219), G253 (= G254), T254 (= T255)
1i1lA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase. (see paper)
52% identity, 98% coverage: 3:300/303 of query aligns to 1:300/304 of 1i1lA
- active site: K156 (= K157)
- binding 2-methylleucine: Y92 (= Y94), K156 (= K157), T254 (= T255), A255 (= A256)
- binding pyridoxal-5'-phosphate: R56 (= R58), K156 (= K157), Y161 (= Y162), E190 (= E191), G193 (= G194), G216 (= G217), I217 (= I218), T218 (= T219), T254 (= T255)
2ej0B Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase with pyridoxamine 5'-phosphate
51% identity, 99% coverage: 3:303/303 of query aligns to 3:304/305 of 2ej0B
- active site: F35 (= F35), G37 (= G37), K158 (= K157), E192 (= E191), L215 (= L215)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R58 (= R58), Y163 (= Y162), E192 (= E191), G195 (= G194), E196 (= E195), L215 (= L215), G217 (= G217), I218 (= I218), T219 (= T219), G254 (= G254), T255 (= T255)
2ej2A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with n-(5'-phosphopyridoxyl)-l-glutamate
50% identity, 99% coverage: 3:303/303 of query aligns to 3:293/294 of 2ej2A
- active site: F35 (= F35), G37 (= G37), K147 (= K157), E181 (= E191), L204 (= L215)
- binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: R58 (= R58), Y94 (= Y94), Y152 (= Y162), E181 (= E191), G184 (= G194), E185 (= E195), L204 (= L215), G206 (= G217), I207 (= I218), T208 (= T219), T244 (= T255), A245 (= A256)
2ej3A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with gabapentin
49% identity, 99% coverage: 3:303/303 of query aligns to 3:296/297 of 2ej3A
- active site: F35 (= F35), G37 (= G37), K150 (= K157), E184 (= E191), L207 (= L215)
- binding [1-(aminomethyl)cyclohexyl]acetic acid: G187 (= G194), G246 (= G254), T247 (= T255), A248 (= A256)
- binding pyridoxal-5'-phosphate: R58 (= R58), K150 (= K157), Y155 (= Y162), E184 (= E191), G187 (= G194), L207 (= L215), G209 (= G217), I210 (= I218), T211 (= T219), G246 (= G254), T247 (= T255)
2eiyA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with 4-methylvaleric acid
49% identity, 99% coverage: 3:303/303 of query aligns to 3:296/297 of 2eiyA
- active site: F35 (= F35), G37 (= G37), K150 (= K157), E184 (= E191), L207 (= L215)
- binding 4-methyl valeric acid: F35 (= F35), Y94 (= Y94), T247 (= T255), A248 (= A256)
- binding pyridoxal-5'-phosphate: R58 (= R58), K150 (= K157), Y155 (= Y162), E184 (= E191), G187 (= G194), E188 (= E195), L207 (= L215), G209 (= G217), I210 (= I218), T211 (= T219), G246 (= G254), T247 (= T255)
1wrvA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase
49% identity, 99% coverage: 3:303/303 of query aligns to 3:296/297 of 1wrvA
- active site: F35 (= F35), G37 (= G37), K150 (= K157), E184 (= E191), L207 (= L215)
- binding pyridoxal-5'-phosphate: R58 (= R58), K150 (= K157), Y155 (= Y162), E184 (= E191), G187 (= G194), L207 (= L215), G209 (= G217), I210 (= I218), T211 (= T219), T247 (= T255)
4whxA X-ray crystal structure of an amino acid aminotransferase from burkholderia pseudomallei bound to the co-factor pyridoxal phosphate
48% identity, 100% coverage: 2:303/303 of query aligns to 4:306/306 of 4whxA
6thqB Crystal structure of branched-chain aminotransferase from thermophilic archaea thermoproteus uzoniensis with norvaline
43% identity, 98% coverage: 4:301/303 of query aligns to 6:298/301 of 6thqB
- active site: F37 (= F35), K156 (= K157), E190 (= E191), L214 (= L215)
- binding pyridoxal-5'-phosphate: R60 (= R58), K156 (= K157), Y161 (= Y162), E190 (= E191), N195 (= N196), L214 (= L215), G216 (= G217), I217 (= I218), T218 (= T219), T254 (= T255)
- binding 2-[o-phosphonopyridoxyl]-amino-pentanoic acid: R60 (= R58), Y97 (= Y94), K156 (= K157), Y161 (= Y162), E190 (= E191), G193 (= G194), E194 (= E195), N195 (= N196), G216 (= G217), I217 (= I218), T218 (= T219), G253 (= G254), T254 (= T255), A255 (= A256)
5mr0D Thermophilic archaeal branched-chain amino acid transaminases from geoglobus acetivorans and archaeoglobus fulgidus: biochemical and structural characterisation (see paper)
42% identity, 92% coverage: 7:286/303 of query aligns to 4:277/290 of 5mr0D
- active site: F32 (= F35), G34 (= G37), K150 (= K157), E183 (= E191), L206 (= L215)
- binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: R51 (= R58), G100 (= G107), L101 (≠ V108), K150 (= K157), Y154 (= Y162), E183 (= E191), G186 (= G194), D187 (≠ E195), L206 (= L215), I209 (= I218), T210 (= T219), G245 (= G254), T246 (= T255)
5e25A Crystal structure of branched-chain aminotransferase from thermophilic archaea geoglobus acetivorans complexed with alpha-ketoglutarate (see paper)
41% identity, 92% coverage: 7:286/303 of query aligns to 5:278/290 of 5e25A
- active site: F33 (= F35), G35 (= G37), K151 (= K157), E184 (= E191), L207 (= L215)
- binding 2-oxoglutaric acid: Y88 (= Y94), K151 (= K157), T247 (= T255), A248 (= A256)
- binding pyridoxal-5'-phosphate: R52 (= R58), K151 (= K157), Y155 (= Y162), E184 (= E191), G187 (= G194), D188 (≠ E195), L207 (= L215), G209 (= G217), I210 (= I218), T211 (= T219), G246 (= G254), T247 (= T255)
6q8eA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum in pmp-form (see paper)
38% identity, 97% coverage: 7:301/303 of query aligns to 6:302/307 of 6q8eA
- active site: F34 (= F35), K156 (= K157), E190 (= E191), L214 (= L215)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R59 (= R58), K156 (= K157), Y161 (= Y162), E190 (= E191), G193 (= G194), S194 (≠ E195), C195 (≠ N196), L214 (= L215), S216 (≠ G217), I217 (= I218), T218 (= T219), G254 (= G254), T255 (= T255)
7neaA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum (m3 mutant). (see paper)
37% identity, 97% coverage: 7:301/303 of query aligns to 6:302/309 of 7neaA
- active site: F34 (= F35), K156 (= K157), E190 (= E191), L214 (= L215)
- binding pyridoxal-5'-phosphate: R59 (= R58), K156 (= K157), Y161 (= Y162), E190 (= E191), G193 (= G194), S194 (≠ E195), L214 (= L215), S216 (≠ G217), I217 (= I218), T218 (= T219), T255 (= T255)
6h65C Crystal structure of the branched-chain-amino-acid aminotransferase from haliangium ochraceum
31% identity, 97% coverage: 8:300/303 of query aligns to 8:303/308 of 6h65C
- active site: F35 (= F35), K158 (= K157), E192 (= E191), L216 (= L215)
- binding pyridoxal-5'-phosphate: R60 (= R58), K158 (= K157), Y163 (= Y162), E192 (= E191), A196 (≠ E195), L216 (= L215), S218 (≠ G217), V219 (≠ I218), T220 (= T219), G256 (= G254), T257 (= T255)
7p3tB Transaminase of gamma-proteobacterium (see paper)
30% identity, 92% coverage: 7:286/303 of query aligns to 6:279/299 of 7p3tB
- binding pyridoxal-5'-phosphate: R53 (= R58), K153 (= K157), R157 (≠ Y162), E186 (= E191), S187 (≠ G192), A188 (≠ S193), A189 (≠ G194), S190 (≠ E195), G210 (= G217), I211 (= I218), T212 (= T219), T248 (= T255)
6xwbA Crystal structure of an r-selective transaminase from thermomyces stellatus. (see paper)
29% identity, 93% coverage: 8:289/303 of query aligns to 31:307/319 of 6xwbA
- binding pyridoxal-5'-phosphate: R77 (= R58), K178 (≠ A156), F185 (≠ Y162), E211 (= E191), G214 (= G194), F215 (≠ E195), N216 (= N196), L233 (= L215), G235 (= G217), V236 (≠ I218), T237 (= T219), T273 (= T255)
7z79B Crystal structure of aminotransferase-like protein from variovorax paradoxus
27% identity, 99% coverage: 2:300/303 of query aligns to 13:305/306 of 7z79B
- binding pyridoxal-5'-phosphate: W46 (≠ F35), R65 (= R58), N166 (≠ K157), S202 (≠ G194), T203 (≠ E195), F222 (≠ L215), G224 (= G217), I225 (= I218), T226 (= T219), G261 (= G254), T262 (= T255)
4ce5B First crystal structure of an (r)-selective omega-transaminase from aspergillus terreus (see paper)
27% identity, 92% coverage: 8:286/303 of query aligns to 33:306/325 of 4ce5B
- active site: Y60 (≠ F35), K180 (= K157), E213 (= E191), L235 (= L215)
- binding calcium ion: D67 (= D42), R69 (≠ A48)
- binding n-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-d-glutamic acid: V62 (≠ G37), R79 (= R58), K180 (= K157), W184 (≠ N164), E213 (= E191), G216 (= G194), F217 (≠ E195), N218 (= N196), L235 (= L215), G237 (= G217), V238 (≠ I218), T239 (= T219), T274 (≠ G254), T275 (= T255), A276 (= A256)
- binding pyridoxal-5'-phosphate: R79 (= R58), K180 (= K157), E213 (= E191), G216 (= G194), F217 (≠ E195), L235 (= L215), G237 (= G217), V238 (≠ I218), T239 (= T219), T275 (= T255)
Query Sequence
>WP_012505142.1 NCBI__GCF_000020625.1:WP_012505142.1
MNKSEKIWMNGELVNWHDAKIHILSHVIHYGSSTFEGIRCYDTDKGSAILFFDEHIKRLY
QSSKIYRMEIPYSEQELKDAIIATIKANGHKACYIRPLVKRGQGALGVNPALASIEVAIA
TWEWGSYLGEEVLENGVDVCVSSWARLAPNTLPTWAKAGGNYLNSQLIKMEALMDNYAEG
IGLDVHGYVAEGSGENIFVIRDGIIYTPMSGQSILPGITRNAVIHIARELGYEVQETLIP
REALYIADEVFLTGTAAEITPVRSIDKYAVGDEKRGPITELLQSKYLDIVKKGTDPYNWL
TFI
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory