SitesBLAST

1zyuA Crystal structure of mycobacterium tuberculosis shikimate kinase in complex with shikimate and amppcp at 2.85 angstrom resolution (see paper)
31% identity, 86% coverage: 9:177/197 of query aligns to 6:166/168 of 1zyuA

query
sites
1zyuA

L

L

T

V

G

G

F

L

S
x
P

G
|
G

S

S

G
|
G

K
|
K

S
|
S

T
|
T

I

I

G

G

P

R

L

R

L

L

A

A

N

K

S

A

L

L

G

G

Y

V

D

G

F

L

V

L

D

D

L

T

D
|
D

Q

V

Q

A

I

I

E

E

H

Q

L

R

A

T

G

G

K

R

T

S

I

I

N

A

R

D

I

I

F

F

T

A

E

T

E

D

G

G

E

E

A

Q

H

E

F

F

R
|
R

D

R

L

I

E

E

L

E

M

D

V

V

L

V

Q

R

N

A

Y

A

S

L

G

A

K

D

S

H

E

D

L

G

V

V

V

L

S

S

L

L

G

G

G
|
G

L

A

L

V

Q

T

N

S

D

P

R

G

C

V

F

R

S

A

L

A

I

L

I

A

S

G

T

H

G

-

T

T

L

V

V

V

Y

Y

L

L

H

E

S

I

N

S

P

A

L

A

V

E

L

G

A

V

K

R

R
|
R

L

T

S

G

H

G

K

N

S

T

D

V

R

R

P
|
P

L
|
L

M

L

K

A

G

G

E

P

D

D

G

-

Q

-

R

-

L

-

S

-

R

-

D

-

A

-

I

R

E

A

Q

E

K

K

I

Y

L

R

N

A

M

L

L

M

E

A

Q

K

R
|
R

E

A

P

P

R

L

Y

Y

-

R

K

R

T

V

A

A

Q

T

I

M

T

R

V

V

E

D

T
|
T

D

N

T

R

K
x
R

R

N

I
x
P

G

G

T

A

T

V

V

V

E

R

E

H

L

I

T

L

R

S

K

R

I

L

E

Q

binding phosphomethylphosphonic acid adenylate ester: P10 (≠ S13), G11 (= G14), G13 (= G16), K14 (= K17), S15 (= S18), T16 (= T19), R109 (= R113), T149 (= T160), R152 (≠ K163), P154 (≠ I165)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D36), R57 (= R60), G80 (= G83), P117 (= P121), L118 (= L122), R135 (= R147)

2iywA Shikimate kinase from mycobacterium tuberculosis in complex with mgatp, open lid (conf. B) (see paper)
31% identity, 86% coverage: 9:177/197 of query aligns to 6:166/178 of 2iywA

query
sites
2iywA

L

L

T

V

G

G

F

L

S

P

G
|
G

S

S

G
|
G

K
|
K

S
|
S

T
|
T

I

I

G

G

P

R

L

R

L

L

A

A

N

K

S

A

L

L

G

G

Y

V

D

G

F

L

V

L

D

D

L

T

D

D

Q

V

Q

A

I

I

E

E

H

Q

L

R

A

T

G

G

K

R

T

S

I

I

N

A

R

D

I

I

F

F

T

A

E

T

E

D

G

G

E

E

A

Q

H

E

F

F

R

R

D

R

L

I

E

E

L

E

M

D

V

V

L

V

Q

R

N

A

Y

A

S

L

G

A

K

D

S

H

E

D

L

G

V

V

V

L

S

S

L

L

G

G

L

A

L

V

Q

T

N

S

D

P

R

G

C

V

F

R

S

A

L

A

I

L

I

A

S

G

T

H

G

-

T

T

L

V

V

V

Y

Y

L

L

H

E

S

I

N

S

P

A

L

A

V

E

L

G

A

V

K

R

R
|
R

L

T

S

G

H

G

K

N

S

T

D

V

R

R

P

P

L

L

M

L

K

A

G

G

E

P

D

D

G

-

Q

-

R

-

L

-

S

-

R

-

D

-

A

-

I

R

E

A

Q

E

K

K

I

Y

L

R

N

A

M

L

L

M

E

A

Q

K

R

R

E

A

P

P

R

L

Y

Y

-

R

K

R

T

V

A

A

Q

T

I

M

T

R

V

V

E

D

T

T

D

N

T

R

K
x
R

R

N

I
x
P

G

G

T

A

T

V

V

V

E

R

E

H

L

I

T

L

R

S

K

R

I

L

E

Q

binding adenosine-5'-triphosphate: G11 (= G14), G13 (= G16), K14 (= K17), S15 (= S18), T16 (= T19), R109 (= R113), R152 (≠ K163), P154 (≠ I165)

2iyvA Shikimate kinase from mycobacterium tuberculosis in complex with adp, open lid (conf. B) (see paper)
31% identity, 86% coverage: 9:177/197 of query aligns to 6:166/179 of 2iyvA

query
sites
2iyvA

L

L

T

V

G

G

F

L

S

P

G
|
G

S

S

G
|
G

K
|
K

S
|
S

T
|
T

I

I

G

G

P

R

L

R

L

L

A

A

N

K

S

A

L

L

G

G

Y

V

D

G

F

L

V

L

D

D

L

T

D

D

Q

V

Q

A

I

I

E

E

H

Q

L

R

A

T

G

G

K

R

T

S

I

I

N

A

R

D

I

I

F

F

T

A

E

T

E

D

G

G

E

E

A

Q

H

E

F

F

R

R

D

R

L

I

E

E

L

E

M

D

V

V

L

V

Q

R

N

A

Y

A

S

L

G

A

K

D

S

H

E

D

L

G

V

V

V

L

S

S

L

L

G

G

L

A

L

V

Q

T

N

S

D

P

R

G

C

V

F

R

S

A

L

A

I

L

I

A

S

G

T

H

G

-

T

T

L

V

V

V

Y

Y

L

L

H

E

S

I

N

S

P

A

L

A

V

E

L

G

A

V

K

R

R
|
R

L

T

S

G

H

G

K

N

S

T

D

V

R

R

P

P

L

L

M

L

K

A

G

G

E

P

D

D

G

-

Q

-

R

-

L

-

S

-

R

-

D

-

A

-

I

R

E

A

Q

E

K

K

I

Y

L

R

N

A

M

L

L

M

E

A

Q

K

R

R

E

A

P

P

R

L

Y

Y

-

R

K

R

T

V

A

A

Q

T

I

M

T

R

V

V

E

D

T

T

D

N

T

R

K
x
R

R

N

I
x
P

G

G

T

A

T

V

V

V

E

R

E

H

L

I

T

L

R

S

K

R

I

L

E

Q

binding adenosine-5'-diphosphate: G11 (= G14), G13 (= G16), K14 (= K17), S15 (= S18), T16 (= T19), R109 (= R113), R152 (≠ K163), P154 (≠ I165)

3bafA Crystal structure of shikimate kinase from mycobacterium tuberculosis in complex with amp-pnp
31% identity, 85% coverage: 9:176/197 of query aligns to 6:165/165 of 3bafA

query
sites
3bafA

L

L

T

V

G

G

F

L

S
x
P

G

G

S

S

G
|
G

K
|
K

S
|
S

T
|
T

I

I

G

G

P

R

L

R

L

L

A

A

N

K

S

A

L

L

G

G

Y

V

D

G

F

L

V

L

D

D

L

T

D
|
D

Q

V

Q

A

I

I

E

E

H

Q

L

R

A

T

G

G

K

R

T

S

I

I

N

A

R

D

I

I

F

F

T

A

E

T

E

D

G

G

E

E

A

Q

H

E

F

F

R

R

D

R

L

I

E

E

L

E

M

D

V

V

L

V

Q

R

N

A

Y

A

S

L

G

A

K

D

S

H

E

D

L

G

V

V

V

L

S

S

L

L

G

G

L

A

L

V

Q

T

N

S

D

P

R

G

C

V

F

R

S

A

L

A

I

L

I

A

S

G

T

H

G

-

T

T

L

V

V

V

Y

Y

L

L

H

E

S

I

N

S

P

A

L

A

V

E

L

G

A

V

K

R

R
|
R

L

T

S

G

H

G

K

N

S

T

D

V

R
|
R

P

P

L

L

M

L

K

A

G

G

E

P

D

D

G

-

Q

-

R

-

L

-

S

-

R

-

D

-

A

-

I

R

E

A

Q

E

K

K

I

Y

L

R

N

A

M

L

L

M

E

A

Q

K

R
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R

E

A

P

P

R

L

Y

Y

-

R

K

R

T

V

A

A

Q

T

I

M

T

R

V

V

E

D

T

T

D

N

T

R

K

R

R

N

I

P

G

G

T

A

T

V

V

V

E

R

E

H

L

I

T

L

R

S

K

R

I

L

binding phosphoaminophosphonic acid-adenylate ester: P10 (≠ S13), G13 (= G16), K14 (= K17), S15 (= S18), T16 (= T19), R109 (= R113), R116 (= R120)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D36), R135 (= R147)

2dfnA Structure of shikimate kinase from mycobacterium tuberculosis complexed with adp and shikimate at 1.9 angstrons of resolution (see paper)
31% identity, 85% coverage: 9:176/197 of query aligns to 6:165/165 of 2dfnA

query
sites
2dfnA

L

L

T

V

G

G

F

L

S
x
P

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

I

I

G

G

P

R

L

R

L

L

A

A

N

K

S

A

L

L

G

G

Y

V

D

G

F

L

V

L

D

D

L

T

D
|
D

Q

V

Q

A

I

I

E

E

H

Q

L

R

A

T

G

G

K

R

T

S

I
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I

N

A

R

D

I

I

F

F

T

A

E

T

E

D

G

G

E

E

A

Q

H

E

F
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F

R
|
R

D

R

L

I

E

E

L

E

M

D

V

V

L

V

Q

R

N

A

Y

A

S

L

G

A

K

D

S

H

E

D

L

G

V

V

V

L

S

S

L

L

G
|
G

L

A

L

V

Q

T

N

S

D

P

R

G

C

V

F

R

S

A

L

A

I

L

I

A

S

G

T

H

G

-

T

T

L

V

V

V

Y

Y

L

L

H

E

S

I

N

S

P

A

L

A

V

E

L

G

A

V

K

R

R
|
R

L

T

S

G

H

G

K

N

S

T

D

V

R
|
R

P

P

L

L

M

L

K

A

G

G

E

P

D

D

G

-

Q

-

R

-

L

-

S

-

R

-

D

-

A

-

I

R

E

A

Q

E

K

K

I

Y

L

R

N

A

M

L

L

M

E

A

Q

K

R
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R

E

A

P

P

R

L

Y

Y

-

R

K

R

T

V

A

A

Q

T

I

M

T

R

V

V

E

D

T

T

D

N

T

R

K
x
R

R

N

I
x
P

G

G

T

A

T

V

V

V

E

R

E

H

L

I

T

L

R

S

K

R

I

L

binding adenosine-5'-diphosphate: G11 (= G14), S12 (= S15), G13 (= G16), K14 (= K17), S15 (= S18), T16 (= T19), R109 (= R113), R116 (= R120), R152 (≠ K163), P154 (≠ I165)
binding chloride ion: P10 (≠ S13), K14 (= K17), G79 (= G82)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D36), I44 (= I47), F56 (= F59), R57 (= R60), G78 (= G81), G79 (= G82), G80 (= G83), R135 (= R147)

1we2A Crystal structure of shikimate kinase from mycobacterium tuberculosis in complex with mgadp and shikimic acid (see paper)
31% identity, 85% coverage: 9:176/197 of query aligns to 6:165/165 of 1we2A

query
sites
1we2A

L

L

T

V

G

G

F

L

S
x
P

G
|
G

S

S

G
|
G

K
|
K

S
|
S

T
|
T

I

I

G

G

P

R

L

R

L

L

A

A

N

K

S

A

L

L

G

G

Y

V

D

G

F

L

V

L

D

D

L

T

D
|
D

Q

V

Q

A

I

I

E

E

H

Q

L

R

A

T

G

G

K

R

T

S

I
|
I

N

A

R

D

I

I

F
|
F

T

A

E

T

E

D

G

G

E

E

A

Q

H

E

F

F

R

R

D

R

L

I

E

E

L

E

M

D

V

V

L

V

Q

R

N

A

Y

A

S

L

G

A

K

D

S

H

E

D

L

G

V

V

V

L

S

S

L

L

G
|
G

L

A

L

V

Q

T

N

S

D

P

R

G

C

V

F

R

S

A

L

A

I

L

I

A

S

G

T

H

G

-

T

T

L

V

V

V

Y

Y

L

L

H

E

S

I

N

S

P

A

L

A

V

E

L

G

A

V

K

R

R
|
R

L

T

S

G

H

G

K

N

S

T

D

V

R
|
R

P

P

L
|
L

M

L

K

A

G

G

E

P

D

D

G

-

Q

-

R

-

L

-

S

-

R

-

D

-

A

-

I

R

E

A

Q

E

K

K

I

Y

L

R

N

A

M

L

L

M

E

A

Q

K

R
|
R

E

A

P

P

R

L

Y

Y

-

R

K

R

T

V

A

A

Q

T

I

M

T

R

V

V

E

D

T

T

D

N

T

R

K

R

R

N

I

P

G

G

T

A

T

V

V

V

E

R

E

H

L

I

T

L

R

S

K

R

I

L

binding adenosine-5'-diphosphate: P10 (≠ S13), G11 (= G14), G13 (= G16), K14 (= K17), S15 (= S18), T16 (= T19), R109 (= R113), R116 (= R120)
binding 3-dehydroshikimate: D33 (= D36), I44 (= I47), F48 (= F51), G78 (= G81), G79 (= G82), G80 (= G83), L118 (= L122), R135 (= R147)

P10880 Shikimate kinase 2; SK 2; Shikimate kinase II; SKII; EC 2.7.1.71 from Dickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi) (see paper)
33% identity, 89% coverage: 1:176/197 of query aligns to 1:168/173 of P10880

query
sites
P10880

M

M

K

T

Q

E

P

P

S

-

L

-

I

I

Y

F

L

M

T

V

G

G

F

A

S

R

G

G

S
x
C

G

G

K
|
K

S

T

T

T

I

V

G

G

P

R

L

E

L

L

A

A

N

R

S

A

L

L

G

G

Y

Y

D

E

F

F

V

V

D

D

L

T

D
|
D

Q

I

Q

F

I

M

E

Q

H

H

L

T

A

S

G

G

K

M

T

T

I

V

N

A

R

D

I

V

F

V

T

A

E

A

E

E

G

G

E

W

A

P

H

G

F

F

R

R

D

R

L

R

E

E

L

S

M

E

V

A

L

L

Q

Q

N

A

Y

V

S

A

G

T

K

P

S

N

E

R

L

-

V

V

S

A

L

T

G

G

-

M

-

V

L

L

Q

E

N

Q

D

N

R

R

C

Q

F

F

S

-

L

-

I

M

I

R

S

A

T

H

G

G

T

T

L

V

V

V

Y

Y

L

L

H

F

S

A

N

P

P

A

L

E

V

E

L

L

A

A

K

L

R

R

L

L

-

Q

-

A

-

S

S

P

H

Q

K

A

S

H

D

Q

R

R

P

P

L

T

M

L

K

T

G

G

E

R

D

P

G

-

Q

-

R

-

L

-

S

-

R

-

D

-

A

-

I

I

E

A

Q

E

K

E

I

M

L

E

N

A

M

V

L

L

E

R

Q

E

R

R

E

E

P

A

R

L

Y

Y

K

Q

T

D

A

V

Q

A

I

H

T

Y

V

V

E

V

T

D

D

A

T

T

K

Q

R

P

I

P

G

A

T

A

T

I

V

V

E
x
C

E

E

L

L

T

M

R

Q

K

T

I

M

C13 (≠ S15) mutation to S: 66% of wild-type activity. Increase in substrates affinity.
K15 (= K17) mutation to M: Loss of activity. Increased thermostability.
D34 (= D36) mutation to N: Loss of activity.
C162 (≠ E170) mutation to S: No effect on activity and substrates affinity.

2shkB The three-dimensional structure of shikimate kinase from erwinia chrysanthemi complexed with adp (see paper)
35% identity, 72% coverage: 1:142/197 of query aligns to 1:138/162 of 2shkB

query
sites
2shkB

M

M

K

T

Q

E

P

P

S

-

L

-

I

I

Y

F

L

M

T

V

G

G

F

A

S

R

G
|
G

S
x
C

G
|
G

K
|
K

S
x
T

T
|
T

I

V

G

G

P

R

L

E

L

L

A

A

N

R

S

A

L

L

G

G

Y

Y

D

E

F

F

V

V

D

D

L

T

D

D

Q

I

Q

F

I

M

E

Q

H

H

L

T

A

S

G

G

K

M

T

T

I

V

N

A

R

D

I

V

F

V

T

A

E

A

E

E

G

G

E

W

A

P

H

G

F

F

R

R

D

R

L

R

E

E

L

S

M

E

V

A

L

L

Q

Q

N

A

Y

V

S

A

G

T

K

P

S

N

E

R

L

-

V

V

S

A

L

T

G

G

-

M

-

V

L

L

Q

E

N

Q

D

N

R

R

C

Q

F

F

S

-

L

-

I

M

I

R

S

A

T

H

G

G

T

T

L

V

V

V

Y

Y

L

L

H

F

S

A

N

P

P

A

L

E

V

E

L

L

A

A

K

L

R
|
R

L

L

S

-

H

Q

K

L

S

T

D

G

R

R

P

P

L

I

M

A

K

E

G

E

E

M

D

E

G

A

Q

V

R

L

L

R

S

E

R

R

D

E

A

A

I

L

E

Y

Q
|
Q

K

D

I

V

L
x
A

N

H

binding adenosine-5'-diphosphate: G12 (= G14), C13 (≠ S15), G14 (= G16), K15 (= K17), T16 (≠ S18), T17 (= T19), R110 (= R113)
binding magnesium ion: Q134 (= Q138), A137 (≠ L141)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 145, 147

1u8aA Crystal structure of mycobacterium tuberculosis shikimate kinase in complex with shikimate and adp at 2.15 angstrom resolution (see paper)
31% identity, 85% coverage: 9:176/197 of query aligns to 6:163/163 of 1u8aA

query
sites
1u8aA

L

L

T

V

G

G

F

L

S

P

G
|
G

S

S

G
|
G

K
|
K

S
|
S

T
|
T

I

I

G

G

P

R

L

R

L

L

A

A

N

K

S

A

L

L

G

G

Y

V

D

G

F

L

V

L

D

D

L

T

D
|
D

Q

V

Q

A

I

I

E

E

H

Q

L

R

A

T

G

G

K

R

T

S

I

I

N

A

R

D

I

I

F
|
F

T

A

E

T

E

D

G

G

E

E

A

Q

H

E

F

F

R
|
R

D

R

L

I

E

E

L

E

M

D

V

V

L

V

Q

R

N

A

Y

A

S

L

G

A

K

D

S

H

E

D

L

G

V

V

V

L

S

S

L

L

G

G

G
|
G

L

A

L

V

Q

T

N

S

D

P

R

G

C

V

F

R

S

A

L

A

I

L

I

A

S

G

T

H

G

-

T

T

L

V

V

V

Y

Y

L

L

H

E

S

I

N

S

P

A

L

A

V

E

L

G

A

V

K

R

R
|
R

L

T

S

G

H

-

K

-

S

G

D

V

R

R

P

P

L
|
L

M

L

K

A

G

G

E

P

D

D

G

-

Q

-

R

-

L

-

S

-

R

-

D

-

A

-

I

R

E

A

Q

E

K

K

I

Y

L

R

N

A

M

L

L

M

E

A

Q

K

R
|
R

E

A

P

P

R

L

Y

Y

-

R

K

R

T

V

A

A

Q

T

I

M

T

R

V

V

E

D

T

T

D

N

T

R

K
x
R

R

N

I
x
P

G

G

T

A

T

V

V

V

E

R

E

H

L

I

T

L

R

S

K

R

I

L

binding adenosine-5'-diphosphate: G11 (= G14), G13 (= G16), K14 (= K17), S15 (= S18), T16 (= T19), R109 (= R113), R150 (≠ K163), P152 (≠ I165)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D36), F48 (= F51), R57 (= R60), G79 (= G82), G80 (= G83), L116 (= L122), R133 (= R147)

1e6cA K15m mutant of shikimate kinase from erwinia chrysanthemi (see paper)
32% identity, 89% coverage: 1:176/197 of query aligns to 1:168/170 of 1e6cA

query
sites
1e6cA

M

M

K

T

Q

E

P

P

S

-

L

-

I

I

Y

F

L

M

T

V

G

G

F

A

S
x
R

G
|
G

S

C

G

G

K
x
M

S
x
T

T

T

I

V

G

G

P

R

L

E

L

L

A

A

N

R

S

A

L

L

G

G

Y

Y

D

E

F

F

V

V

D

D

L

T

D

D

Q

I

Q

F

I

M

E

Q

H

H

L

T

A

S

G

G

K

M

T

T

I

V

N

A

R

D

I

V

F

V

T

A

E

A

E

E

G

G

E

W

A

P

H

G

F

F

R

R

D

R

L

R

E

E

L

S

M

E

V

A

L

L

Q

Q

N

A

Y

V

S

A

G

T

K

P

S

N

E

R

L

-

V

V

S

A

L

T

G

G

-

M

-

V

L

L

Q

E

N

Q

D

N

R

R

C

Q

F

F

S

-

L

-

I

M

I

R

S

A

T

H

G

G

T

T

L

V

V

V

Y

Y

L

L

H

F

S

A

N

P

P

A

L

E

V

E

L

L

A

A

K

L

R

R

L

L

S

Q

-

A

-

S

-

L

H

Q

K

A

S

H

D

Q

R

R

P

P

L

T

M

L

K

T

G

G

E

R

D

P

G

-

Q

-

R

-

L

-

S

-

R

-

D

-

A

-

I

I

E

A

Q

E

K

E

I

M

L

E

N

A

M

V

L

L

E

R

Q

E

R

R

E

E

P

A

R

L

Y

Y

K

Q

T

D

A

V

Q

A

I

H

T

Y

V

V

E

V

T

D

D

A

T

T

K

Q

R

P

I

P

G

A

T

A

T

I

V

V

E

C

E

E

L

L

T

M

R

Q

K

T

I

M

binding phosphate ion: R11 (≠ S13), G12 (= G14), M15 (≠ K17), T16 (≠ S18)

1shkA The three-dimensional structure of shikimate kinase from erwinia chrysanthemi (see paper)
40% identity, 58% coverage: 1:114/197 of query aligns to 1:111/158 of 1shkA

query
sites
1shkA

M

M

K

T

Q

E

P

P

S

-

L

-

I

I

Y

F

L

M

T

V

G

G

F

A

S

R

G

G

S

C

G

G

K

K

S

T

T

T

I

V

G

G

P

R

L

E

L

L

A

A

N

R

S

A

L

L

G

G

Y

Y

D

E

F

F

V

V

D

D

L

T

D

D

Q

I

Q

F

I

M

E

Q

H

H

L

T

A

S

G

G

K

M

T

T

I

V

N

A

R

D

I

V

F

V

T

A

E

A

E

E

G

G

E

W

A

P

H

G

F

F

R

R

D

R

L

R

E

E

L

S

M

E

V

A

L

L

Q

Q

N

A

Y

V

S

A

G

T

K

P

S

N

E

R

L

-

V

V

S

A

L

T

G

G

-

M

-

V

L

L

Q

E

N

Q

D

N

R

R

C

Q

F

F

S

-

L

-

I

M

I
x
R

S
x
A

T

H

G
|
G

T

T

L

V

V

V

Y

Y

L

L

H

F

S

A

N

P

P

A

L

E

V

E

L

L

A

A

K

L

R

R

L

L

binding magnesium ion: R92 (≠ I95), A93 (≠ S96), G95 (= G98)

Sites not aligning to the query:

binding magnesium ion: 129, 132

1zuiA Structural basis for shikimate-binding specificity of helicobacter pylori shikimate kinase (see paper)
34% identity, 59% coverage: 7:123/197 of query aligns to 3:116/158 of 1zuiA

query
sites
1zuiA

I

L

Y

V

L

L

T

I

G

G

F

F

S
x
M

G

G

S

S

G

G

K

K

S

S

T

S

I

L

G

A

P

Q

L

E

L

L

A

G

N

L

S

A

L

L

G

K

Y

L

D

E

F

V

V

L

D

D

L

T

D
|
D

Q

M

Q

I

I

I

E

S

H

E

L

R

A

V

G

G

K

L

T

S

I

V

N

R

R

E

I

I

F
|
F

T

E

E

E

E

L

G

G

E

E

A

D

H

N

F

F

R
|
R

D

M

L

F

E

E

L

K

M

N

V

L

L

I

Q

D

N

E

Y

L

-

K

S

T

G

L

K

K

S

T

E

P

L

H

V

V

V

I

S

S

L

T

G
|
G

G

G

L

I

L

V

Q

M

N

H

D

E

R

N

C

L

F

K

S

G

L

L

I

-

S

-

T

-

G

G

T

T

L

T

V

F

Y

Y

L

L

H

K

S

M

N

D

P

F

L

E

V

T

L

L

A

I

K

K

R

R

L

L

S

N

H

Q

K

R

S
x
E

D

K

R
|
R

P

P

L

L

M

L

binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: M9 (≠ S13), D32 (= D36), F47 (= F51), R56 (= R60), G78 (= G81), G79 (= G82), E111 (≠ S118), R113 (= R120)

Sites not aligning to the query:

binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: 129

6hqvA Pentafunctional arom complex from chaetomium thermophilum (see paper)
30% identity, 74% coverage: 7:151/197 of query aligns to 845:983/1555 of 6hqvA

query
sites
6hqvA

I

I

Y

F

L

I

T

V

G

G

F

M

S

R

G

G

S

A

G

G

K

K

S

S

T

T

I

A

G

G

P

R

L

W

L

M

A

S

N

E

S

L

L

L

G

K

Y

R

D

P

F

L

V

V

D

D

L

L

D
|
D

Q

A

Q

E

I

L

E

E

H

R

L

R

A

E

G

G

K

M

T

T

I

I

N

P

R

E

I

I

F

I

T

R

-

G

E

E

E

R

G

G

E

W

A

E

H

G

F

F

R

R

D

Q

L

A

E

E

L

L

M

E

V

L

L

L

Q

Q

N

D

Y

V

-

I

-

K

S

N

G

Q

K

S

S

K

E

G

L

Y

V

I

V

F

S

S

L

C

G

G

G
|
G

L

I

L

V

Q

E

N

T

D

E

R

A

C

A

F

R

S

K

L

L

I

L

I

I

S

D

T

-

G

-

T

-

L

-

V

-

Y

-

L

Y

H

H

S

K

N

N

-

G

P

P

L

V

V

L

L

L

A

V

K

H

R

R

L

D

S

T

H

D

K

Q

S

V

D

V

R

E

P

Y

L

L

M

M

K

R

G

D

E

K

D

-

G

-

Q

-

R

-

L

-

S

T

R

R

D

P

A

A

I

Y

E

S

Q

E

K

N

I

I

L

R

N

E

M

V

L

Y

E

E

Q

R

R
|
R

E

K

P

P

R

W

Y

F

binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D874 (= D36), G923 (= G82), G924 (= G83), R979 (= R147)

Sites not aligning to the query:

active site: 123, 145, 187, 243, 253, 257, 261, 264, 268, 280
binding (4S,5R)-4,5-dihydroxy-3-oxocyclohex-1-ene-1-carboxylic acid: 1060, 1062, 1181, 1224, 1232, 1242, 1243
binding glutamic acid: 139, 145, 187, 243, 257, 264, 280
binding nicotinamide-adenine-dinucleotide: 42, 44, 45, 76, 79, 107, 108, 109, 112, 132, 133, 135, 139, 140, 145, 154, 175, 176, 177, 180, 280
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: 413, 562, 563, 1277, 1279, 1323, 1327, 1348, 1368, 1526
binding zinc ion: 187, 264, 280

3n2eA Crystal structure of helicobactor pylori shikimate kinase in complex with nsc162535 (see paper)
34% identity, 59% coverage: 7:123/197 of query aligns to 4:119/168 of 3n2eA

query
sites
3n2eA

I

L

Y

V

L

L

T

I

G

G

F
|
F

S

M

G

G

S

S

G

G

K

K

S

S

T

S

I

L

G

A

P

Q

L

E

L

L

A

G

N

L

S

A

L

L

G

K

Y

L

D

E

F

V

V

L

D

D

L

T

D

D

Q

M

Q

I

I

I

E

S

H

E

L

R

A

V

G

G

K

L

T

S

I

V

N
x
R

R

E

I

I

F
|
F

T

E

E

E

E

L

G

G

E

E

A

D

H

N

F

F

R
|
R

D

M

L

F

E

E

L

K

M

N

V

L

L

I

Q

D

N

E

Y

L

-

K

S

T

G

L

K

K

S

T

E

P

L

H

V

V

V

I

S

S

L

T

G

G

G
|
G

L

I

L

V

Q
x
M

N

H

D

E

R

N

C

L

F

K

S

G

L

L

I

-

S

-

T

-

G

G

T

T

L

T

V

F

Y

Y

L

L

H

K

S

M

N

D

P

F

L

E

V

T

L

L

A

I

K

K

R

R

L

L

S

N

H

Q

K

K

-

E

-

R

S

A

D

K

R
|
R

P

P

L

L

M

L

binding 7-amino-4-hydroxy-3-[(E)-(5-hydroxy-7-sulfonaphthalen-2-yl)diazenyl]naphthalene-2-sulfonic acid: F9 (= F12), R45 (≠ N48), F48 (= F51), R57 (= R60), G80 (= G82), G81 (= G83), M84 (≠ Q86), R116 (= R120)

Sites not aligning to the query:

binding 7-amino-4-hydroxy-3-[(E)-(5-hydroxy-7-sulfonaphthalen-2-yl)diazenyl]naphthalene-2-sulfonic acid: 132, 135, 136

3mufA Shikimate kinase from helicobacter pylori in complex with shikimate-3- phosphate and adp (see paper)
34% identity, 59% coverage: 7:123/197 of query aligns to 3:118/160 of 3mufA

query
sites
3mufA

I

L

Y

V

L

L

T

I

G

G

F

F

S
x
M

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
x
S

I

L

G

A

P

Q

L

E

L

L

A

G

N

L

S

A

L

L

G

K

Y

L

D

E

F

V

V

L

D

D

L

T

D
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binding adenosine-5'-diphosphate: G10 (= G14), S11 (= S15), G12 (= G16), K13 (= K17), S14 (= S18), S15 (≠ T19), M98 (≠ S105), R106 (= R113)
binding shikimate-3-phosphate: M9 (≠ S13), D32 (= D36), R56 (= R60), G78 (= G81), G79 (= G82), G80 (= G83), R115 (= R120)

Sites not aligning to the query:

P07547 Pentafunctional AROM polypeptide; EC 4.2.3.4; EC 2.5.1.19; EC 2.7.1.71; EC 4.2.1.10; EC 1.1.1.25 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see 2 papers)
37% identity, 41% coverage: 7:86/197 of query aligns to 866:947/1583 of P07547

query
sites
P07547

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Sites not aligning to the query:

44:46 binding NAD(+)
81:84 binding NAD(+)
114:116 binding NAD(+)
119 binding NAD(+)
139:140 binding NAD(+)
161 binding NAD(+)
179:182 binding NAD(+)
190 binding NAD(+)
194 binding Zn(2+)
271 binding Zn(2+)
287 binding Zn(2+)

7tbvA Crystal structure of the shikimate kinase + 3-dehydroquinate dehydratase + 3-dehydroshikimate dehydrogenase domains of aro1 from candida albicans (see paper)
30% identity, 50% coverage: 7:104/197 of query aligns to 6:109/682 of 7tbvA

query
sites
7tbvA

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Sites not aligning to the query:

binding magnesium ion: 300, 302, 329

Query Sequence

>WP_012506069.1 NCBI__GCF_000020625.1:WP_012506069.1
MKQPSLIYLTGFSGSGKSTIGPLLANSLGYDFVDLDQQIEHLAGKTINRIFTEEGEAHFR
DLELMVLQNYSGKSELVVSLGGGLLQNDRCFSLIISTGTLVYLHSNPLVLAKRLSHKSDR
PLMKGEDGQRLSRDAIEQKILNMLEQREPRYKTAQITVETDTKRIGTTVEELTRKIERYI
RRCEKKQLERNTKQRKQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory