SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012506546.1 NCBI__GCF_000020625.1:WP_012506546.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P9WP55 O-acetylserine sulfhydrylase; OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase; EC 2.5.1.47 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
65% identity, 100% coverage: 1:307/308 of query aligns to 1:307/310 of P9WP55

query
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P9WP55

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K44 (= K44) modified: N6-(pyridoxal phosphate)lysine
N74 (= N74) binding pyridoxal 5'-phosphate
GTGGT 178:182 (= GTGGT 178:182) binding pyridoxal 5'-phosphate
S266 (= S266) binding pyridoxal 5'-phosphate

2q3dA 2.2 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) from mycobacterium tuberculosis in complex with the reaction intermediate alpha-aminoacrylate (see paper)
65% identity, 99% coverage: 1:306/308 of query aligns to 1:306/306 of 2q3dA

query
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2q3dA

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active site: K44 (= K44), S266 (= S266), P293 (= P293)
binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: K44 (= K44), T71 (= T71), S72 (= S72), N74 (= N74), T75 (= T75), Q144 (= Q144), V177 (= V177), G178 (= G178), T179 (= T179), G180 (= G180), T182 (= T182), G222 (= G222), I223 (= I223), S266 (= S266), P293 (= P293), D294 (= D294)

3zeiA Structure of the mycobacterium tuberculosis o-acetylserine sulfhydrylase (oass) cysk1 in complex with a small molecule inhibitor (see paper)
65% identity, 97% coverage: 1:300/308 of query aligns to 1:300/300 of 3zeiA

query
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3zeiA

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active site: K44 (= K44), S266 (= S266), P293 (= P293)
binding 3-[(Z)-[(5Z)-5-[[2-(2-hydroxy-2-oxoethyloxy)phenyl]methylidene]-3-methyl-4-oxidanylidene-1,3-thiazolidin-2-ylidene]amino]benzoic acid: T71 (= T71), S72 (= S72), I126 (= I126), Q144 (= Q144), F145 (= F145), K215 (≠ P215), G222 (= G222), A225 (= A225), F227 (= F227)
binding pyridoxal-5'-phosphate: K44 (= K44), N74 (= N74), V177 (= V177), G178 (= G178), T179 (= T179), G180 (= G180), T182 (= T182), G222 (= G222), S266 (= S266), P293 (= P293), D294 (= D294)

2q3cA 2.1 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) holoenzyme from mycobacterium tuberculosis in complex with the inhibitory peptide dfsi (see paper)
65% identity, 97% coverage: 1:300/308 of query aligns to 1:300/300 of 2q3cA

query
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2q3cA

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L

active site: K44 (= K44), S266 (= S266), P293 (= P293)
binding : T71 (= T71), S72 (= S72), G73 (= G73), T75 (= T75), M122 (= M122), Q144 (= Q144), K215 (≠ P215), G222 (= G222), A225 (= A225)

4lmaA Crystal structure analysis of o-acetylserine sulfhydrylase cysk1 from microcystis aeruginosa 7806 (see paper)
61% identity, 100% coverage: 1:307/308 of query aligns to 1:310/318 of 4lmaA

query
sites
4lmaA

M

M

N

R

V

I

Y

A

E

H

S

D

I

V

T

T

E

E

T

L

V

V

G

G

R

R

T

T

P

P

L

L

V

V

R

Q

I

L

R

N

R

R

M

I

-

P

-

Q

A

A

D

E

H

G

S

C

Y

L

A

G

D

R

V

I

Y

V

A

M

K

K

L

L

E

E

S

G

F

M

N

N

P

P

L

A

S

A

S

S

V

V

K
|
K

D

D

R

R

V

I

G

G

V

T

A

H

M

M

I

I

E

N

D

S

A

A

E

E

R

K

N

A

G

G

L

L

I

I

G

N

P

P

E

E

T

T

I

V

-

L

I

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

F

M

S

T

C

A

A

A

S

A

R

K

G

G

Y

Y

R

R

L

L

I

I

L

L

T

T

M

M

P

P

D

E

T

T

M

M

S

S

V

L

E

E

R

R

R

A

L

M

L

L

K

K

I

A

L

Y

G

G

A

A

E

T

L

L

V

E

L

L

T

T

E

P

G

G

A

S

A

Q

G

G

M

M

K

K

G

G

A

A

I

I

A

L

E

R

A

A

E

Q

R

Q

L

I

V

V

D

D

S

S

I

I

P

P

H

G

S

A

L

Y

M

M

L

L

Q

Q

F

F

N

R

N

N

P

P

A

S

N

N

P

P

E

E

T

I

H

H

R

R

R

L

T

T

A

A

R

E

E

E

I

I

W

W

D

Q

D

D

T

T

D

E

G

G

Q

Q

V

V

D

D

I

F

F

I

I

V

A

A

G

G

V

V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

T

T

G

G

V

V

G

A

S

E

V

V

L

I

R

K

E

S

L

R

K

K

P

P

G

S

V

F

H

Q

I

V

V

V

A

A

V

V

E

E

P

P

D

F

D

N

S

S

P

P

V

V

I

I

S

S

G

G

E

N

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

G

E

N

V

L

L

D

R

P

T

S

D

V

L

I

I

D

D

E

E

I

V

I

I

T

T

V

V

S

S

N

D

Q

E

D

E

A

A

A

F

E

Q

T

F

A

G

R

R

K

R

L

L

A

A

R

K

K

E

E

E

G

G

I

L

L

L

C

S

G

G

I

I

S
|
S

S

S

G

G

A

A

A

N

M

L

W

C

A

A

M

I

S

Q

V

L

A

A

A

Q

R

R

P

P

A

E

M

N

E

E

G

G

K

K

N

L

I

I

V

V

L

I

L

Q

P
|
P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

W

L

L

M

F

F

A

Q

D

N

active site: K46 (= K44), S269 (= S266)
binding pyridoxal-5'-phosphate: K46 (= K44), N77 (= N74), G181 (= G178), T182 (= T179), G183 (= G180), T185 (= T182), G225 (= G222), S269 (= S266), P296 (= P293)

5xoqA Crystal structure of o-acetylserine sulfhydrylase with bound transcription factor peptide inhibitor from planctomyces limnophilus
61% identity, 100% coverage: 1:307/308 of query aligns to 2:308/310 of 5xoqA

query
sites
5xoqA

M

M

N

P

V

I

Y

F

E

K

S

D

I

N

T

S

E

E

T

S

V

I

G

G

R

R

T

T

P

P

L

L

V

V

R

Q

I

I

R

N

R

R

M

L

A

T

D

A

H

G

S

L

Y

S

A

S

D

R

V

V

Y

L

A

A

K

K

L

I

E

E

S

G

F

R

N

N

P

P

L

A

S

Y

S

S

V

V

K

K

D

C

R

R

V

I

G

G

V

A

A

A

M

M

I

I

E

W

D

D

A

A

E

E

R

Q

N

S

G

G

L

K

I

L

G

K

P

P

E

G

T

M

T

H

I

V

E

E

P

P

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

I

I

A

A

L

L

A

A

F

F

S

V

C

C

A

A

S

A

R

R

G

G

Y

Y

R

K

L

L

I

T

L

L

T

T

M

M

P

P

D

E

T

T

M

M

S

S

V

I

E

E

R

R

R

M

L

M

L

L

K

K

I

S

L

F

G

G

A

A

E

D

L

L

V

V

L

L

T

T

E

P

G

G

A

A

A

D

G

G

M
|
M

K

K

G

G

A

A

I

I

A

S

E

K

A

A

E

E

R

E

L

L

V

A

D

A

S

Q

I

-

P

P

H

G

S

W

L

F

M

I

L

P

Q

Q

Q
|
Q

F

F

N

K

N

N

P

P

A

A

N

N

P

P

E

A

T

I

H

H

R

V

R

K

T

T

A

G

R

P

E

E

I

I

W

W

D

N

D

D

T

T

D

E

G

G

Q

Q

V

V

D

D

I

V

F

F

I

V

A

A

G

G

V

V

G

G

T

T

G

G

T

T

I

I

T

T

G

G

V

V

G

A

S

R

V

F

L

L

R

K

-

H

E

E

L

K

K

K

P

H

G

P

V

V

H

H

I

V

V

V

A

A

V

V

E

E

P

P

D

A

S

S

P

P

V

V

I

L

S

A

G

G

E

P

P

A

G

G

P
x
R

H
|
H

K

K

I

I

Q
|
Q

G
|
G

I

I

G

G

A
|
A

G

G

F

F

V

V

P

P

G

D

N

T

L

F

D

D

P

R

S

S

V

V

I

V

D

D

E

E

I

I

I

L

T

S

V

V

S

T

N

D

Q

D

D

E

A

A

A

I

E

E

T

T

A

A

R

R

K

K

L

L

A

A

R

M

K

E

E

E

G

G

I

I

L

S

C

C

G

G

I

I

S

S

S

C

G

G

A

A

M

M

W

A

A

G

A

A

M

L

S

K

V

V

A

A

R

R

P

P

A

E

M

F

E

A

G

G

K

K

N

T

I

I

V

V

V

T

L

V

L

L

P

P

D

D

T

A

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

W

A

L

L

F

F

A

E

D

N

binding : T72 (= T71), S73 (= S72), G74 (= G73), T76 (= T75), M123 (= M122), Q144 (= Q144), R218 (≠ P217), H219 (= H218), Q222 (= Q221), G223 (= G222), A226 (= A225)

4lmbA Crystal structure analysis of o-acetylserine sulfhydrylase cysk2 complexed with cystine from microcystis aeruginosa 7806 (see paper)
59% identity, 100% coverage: 1:307/308 of query aligns to 1:310/310 of 4lmbA

query
sites
4lmbA

M

M

N

L

V

I

Y

A

E

R

S

D

I

I

T

T

E

Q

T

L

V

V

G

G

R

R

T

T

P

P

L

L

V

V

R

Q

I

L

R

N

R

R

M

I

-

P

-

V

A

A

D

E

H

G

S

V

Y

K

A

A

D

R

V

I

Y

V

A

V

K

K

L

L

E

E

S

S

F

M

N

N

P

P

L

A

S

A

S

S

V

V

K
|
K

D

D

R

R

V

I

G

G

V

V

A

S

M

M

I

V

E

E

D

D

A

A

E

E

R

A

N

A

G

G

L

L

I

I

G

H

P

P

E

D

T

K

T

T

I

I

-

L

I

V

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

F

M

S

V

C

A

A

A

S

A

R

K

G

G

Y

Y

R

R

L

L

I

V

L

L

T

T

M

M

P

P

D

E

T

T

M
|
M

S

S

V

L

E

E

R

R

R

A

L

M

L

L

K

K

I

A

L

Y

G

G

A

A

E

Q

L

L

V

E

L

L

T

T

E

P

G

G

A

S

A

Q

G

G

M
|
M

K

E

G

G

A

A

I

I

A

T

E

R

A

A

E

E

R

E

L

I

V

V

D

E

S

N

I

T

P

P

H

N

S

A

L

Y

M

S

L

L

Q

Q

Q
|
Q

F
|
F

N

R

N

N

P

P

A

A

N

N

P

P

E

K

T

I

H

H

R

R

R

E

T

T

A

A

R

E

E

E

I

I

W

W

D

A

D

D

T

T

D

D

G

G

Q

L

V

V

D

D

I

I

F

V

I

I

A

G

G

G

V
|
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

T

T

G

G

V

I

G

A

S

E

V

T

L

I

R

K

E

P

L

R

K

R

P

P

G

Q

V

F

H

Q

I

A

V

I

A

A

V

V

E

E

P

P

D

S

D

N

S

S

P

P

V

V

I

L

S

S

G

G

E

Q

P

P

G

G

P

P

H

H

K

K

I

I

Q
|
Q

G
|
G

I
|
I

G

G

A
|
A

G

G

F

F

V

I

P

P

G

A

N

I

L

F

D

R

P

P

S

E

V

L

I

I

D

D

E

E

I

V

I

I

T

I

V

V

S

D

N

D

Q

T

D

E

A

A

A

F

E

A

T

Y

A

A

R

R

K

R

L

L

A

A

R

R

K

Q

E

E

G

G

I

L

L

L

C

S

G

G

I

I

S
|
S

S

A

G

G

A

A

M

L

W

W

A

A

M

I

S

Q

V

V

A

G

A

K

R

R

P

P

A

E

M

N

E

E

G

D

K

K

N

L

I

I

V

V

V

M

L

I

L

Q

P
|
P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

W

A

L

L

F

F

A

K

D

D

active site: K46 (= K44), S269 (= S266)
binding cysteine: K46 (= K44), T74 (= T71), S75 (= S72), N77 (= N74), T78 (= T75), M101 (= M98), M125 (= M122), M125 (= M122), Q147 (= Q144), F148 (= F145), Q224 (= Q221), G225 (= G222), G225 (= G222), I226 (= I223), A228 (= A225)
binding pyridoxal-5'-phosphate: K46 (= K44), N77 (= N74), V180 (= V177), G181 (= G178), T182 (= T179), G183 (= G180), T185 (= T182), G225 (= G222), S269 (= S266), P296 (= P293)

4aecA Crystal structure of the arabidopsis thaliana o-acetyl-serine-(thiol)- lyasE C (see paper)
57% identity, 99% coverage: 1:305/308 of query aligns to 11:316/323 of 4aecA

query
sites
4aecA

M

L

N

N

V

I

Y

A

E

D

S

N

I

V

T

S

E

Q

T

L

V

I

G

G

R

K

T

T

P

P

L

M

V

V

R

Y

I

L

R

N

R

S

M

I

A

A

D

K

H

G

S

C

Y

V

A

A

D

N

V

I

Y

A

A

A

K

K

L

L

E

E

S

I

F

M

N

E

P

P

L

C

S

C

S

S

V

V

K
|
K

D

D

R

R

V

I

G

G

V

Y

A

S

M

M

I

V

E

T

D

D

A

A

E

E

R

Q

N

K

G

G

L

F

I

I

G

S

P

P

-

G

E

K

T

S

T

V

I

L

I

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

G

L

L

A

A

F

F

S

I

C

A

A

A

S

S

R

R

G

G

Y

Y

R

R

L

L

I

I

L

L

T

T

M

M

P

P

D

A

T

S

M

M

S

S

V

M

E

E

R

R

R

V

L

L

K

K

I

A

L

F

G

G

A

A

E

E

L

L

V

V

L

L

T

T

E

D

G

P

A

A

A

K

G

G

M

M

K

T

G

G

A

A

I

V

A

Q

E

K

A

A

E

E

R

E

L

I

V

L

D

K

S

N

I

T

P

P

H

D

S

A

L

Y

M

M

L

L

Q

Q

F

F

N

D

N

N

P

P

A

A

N

N

P

P

E

K

T

I

H

H

R

Y

R

E

T

T

A

G

R

P

E

E

I

I

W

W

D

D

T

T

D

K

G

G

Q

K

V

V

D

D

I

I

F

F

I

V

A

A

G

G

V
x
I

G
|
G

T
|
T

G
|
G

T
|
T

I

I

T

T

G

G

V

V

G

G

S

R

V

F

L

I

R

K

E

E

L

K

K

N

P

P

G

K

V

T

H

Q

I

V

V

I

A

G

V

V

E

E

P

P

D

T

D

E

S

S

P

D

V

I

I

L

S

S

G

G

E

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

G

K

N

N

L

L

D

D

P

Q

S

K

V

I

I

M

D

D

E

E

I

V

I

I

T

A

V

I

S

S

N

S

Q

E

D

E

A

A

A

I

E

E

T

T

A

A

R

K

K

Q

L

L

A

A

R

L

K

K

E

E

G

G

I

L

L

M

C

V

G

G

I

I

S
|
S

S

S

G

G

A

A

M

A

W

A

A

A

M

I

S

K

V

V

A

A

A

K

R

R

P

P

A

E

M

N

E

A

G

G

K

K

N

L

I

I

V

A

V

V

L

V

L

F

P
|
P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

W

P

L

L

F

F

active site: K54 (= K44), S277 (= S266)
binding pyridoxal-5'-phosphate: K54 (= K44), N85 (= N74), I188 (≠ V177), G189 (= G178), T190 (= T179), G191 (= G180), G192 (= G181), T193 (= T182), G233 (= G222), S277 (= S266), P304 (= P293)

P0A1E3 Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; EC 2.5.1.47 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
59% identity, 99% coverage: 2:307/308 of query aligns to 3:314/323 of P0A1E3

query
sites
P0A1E3

N

K

V

I

Y

Y

E

E

S

D

I

N

T

S

E

L

T

T

V

I

G

G

R

H

T

T

P

P

L

L

V

V

R

R

I

L

R

N

R

R

M

I

A

G

D

N

H

-

S

-

Y

-

A

G

D

R

V

I

Y

L

A

A

K

K

L

V

E

E

S

S

F

R

N

N

P

P

L

S

S

F

S

S

V

V

K

K

D

C

R

R

V

I

G

G

V

A

A

N

M

M

I

I

E

W

D

D

A

A

E

E

R

K

N

R

G

G

L

V

I

L

G

K

P

P

E

G

T

V

T

E

I

L

I

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

F

Y

S

V

C

A

A

A

S

A

R

R

G

G

Y

Y

R

K

L

L

I

T

L

L

T

T

M

M

P

P

D

E

T

T

M

M

S

S

V

I

E

E

R

R

R

K

L

L

K

K

I

A

L

L

G

G

A

A

E

N

L

L

V

V

L

L

T

T

E

E

G

G

A

A

A

K

G

G

M

M

K

K

G

G

A

A

I

I

A

Q

E

K

A

A

E

E

R

E

L

I

V

V

D

A

S

S

I

D

P

P

H

Q

S

K

-

Y

L

L

M

L

L

L

Q

Q

F

F

N

S

N

N

P

P

A

A

N

N

P

P

E

E

T

I

H

H

R

E

R

K

T

T

A

G

R

P

E

E

I

I

W

W

D

E

D

D

T

T

D

D

G

G

Q

Q

V

V

D

D

I

V

F

F

I

I

A

S

G

G

V

V

G

G

T

T

G

G

T

T

I

L

T

T

G

G

V

V

G

T

S

R

V

Y

L

I

R

K

E

G

L

T

K

K

P

G

G

K

V

T

H

D

I

L

-

I

-

T

V

V

A

A

V

V

E

E

P

P

D

T

D

D

S

S

P

P

V

V

I

I

S

A

-

Q

-

A

-

L

G

A

G

G

E

E

-

E

-

I

-

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

G

G

N

N

L

L

D

D

P

L

S

K

V

L

I

I

D

D

E

K

I

V

T

G

V

I

S

T

N

N

Q

E

D

E

A

A

A

I

E

S

T

T

A

A

R

R

K

R

L

L

A

M

R

E

K

E

E

E

G

G

I

I

L

L

C

A

G

G

I

I

S
|
S

S

S

G

G

A

A

M

V

W

A

A

A

M

L

S

K

V

L

A

Q

A

E

R

D

P

E

A

S

M

F

E

T

G

N

K

K

N

N

I

I

V

V

L

I

L

L

P

P

D

S

T

S

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

W

A

L

L

F

F

A

A

D

D

N72 (= N74) binding pyridoxal 5'-phosphate
S273 (= S266) binding pyridoxal 5'-phosphate

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

6z4nAAA structure of oass complexed with upar inhibitor (see paper)
59% identity, 99% coverage: 2:307/308 of query aligns to 4:315/321 of 6z4nAAA

query
sites
6z4nAAA

N

K

V

I

Y

Y

E

E

S

D

I

N

T

S

E

L

T

T

V

I

G

G

R

H

T

T

P

P

L

L

V

V

R

R

I

L

R

N

R

R

M

I

A

G

D

N

H

-

S

-

Y

-

A

G

D

R

V

I

Y

L

A

A

K

K

L

V

E

E

S

S

F

R

N

N

P

P

L

S

S

F

S

S

V

V

K
|
K

D

C

R

R

V

I

G

G

V

A

A

N

M

M

I

I

E

W

D

D

A

A

E

E

R

K

N

R

G

G

L

V

I

L

G

K

P

P

E

G

T

V

T

E

I

L

I

V

E

E

P

P

T
|
T

S

S

G
|
G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

F

Y

S

V

C

A

A

A

S

A

R

R

G

G

Y

Y

R

K

L

L

I

T

L

L

T

T

M

M

P

P

D

E

T

T

M

M

S

S

V

I

E

E

R

R

R

K

L

L

K

K

I

A

L

L

G

G

A

A

E

N

L

L

V

V

L

L

T

T

E

E

G

G

A

A

A

K

G

G

M

M

K

K

G

G

A

A

I

I

A

Q

E

K

A

A

E

E

R

E

L

I

V

V

D

A

S

S

I

D

P

P

H

Q

S

K

-

Y

L

L

M

L

L

L

Q

Q

Q
|
Q

F
|
F

N

S

N

N

P

P

A

A

N

N

P

P

E

E

T

I

H

H

R

E

R

K

T

T

A

G

R

P

E

E

I

I

W

W

D

E

D

D

T

T

D

D

G

G

Q

Q

V

V

D

D

I

V

F

F

I

I

A

S

G

G

V
|
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

L

T

T

G

G

V

V

G

T

S

R

V

Y

L

I

R

K

E

G

L

T

K

K

P

G

G

K

V

T

H

D

I

L

-

I

-

T

V

V

A

A

V

V

E

E

P

P

D

T

D

D

S

S

P

P

V

V

I

I

S

A

-

Q

-

A

-

L

G

A

G

G

E

E

-

E

-

I

-

K

P

P

G

G

P

P

H

H

K

K

I

I

Q
|
Q

G
|
G

I
|
I

G

G

A
|
A

G

G

F

F

V

I

P

P

G

G

N

N

L

L

D

D

P

L

S

K

V

L

I

I

D

D

E

K

I

V

T

G

V

I

S

T

N

N

Q

E

D

E

A

A

A

I

E

S

T

T

A

A

R

R

K

R

L

L

A

M

R

E

K

E

E

E

G

G

I

I

L

L

C

A

G

G

I

I

S
|
S

S

S

G

G

A

A

M

V

W

A

A

A

M

L

S

K

V

L

A

Q

A

E

R

D

P

E

A

S

M

F

E

T

G

N

K

K

N

N

I

I

V

V

L

I

L

L

P
|
P

D

S

T

S

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

W

A

L

L

F

F

A

A

D

D

binding pyridoxal-5'-phosphate: K43 (= K44), N73 (= N74), V177 (= V177), G178 (= G178), T179 (= T179), G180 (= G180), T182 (= T182), G230 (= G222), S274 (= S266), P301 (= P293)
binding (1~{S},2~{S})-1-[(4-methylphenyl)methyl]-2-phenyl-cyclopropane-1-carboxylic acid: K43 (= K44), T70 (= T71), G72 (= G73), N73 (= N74), T74 (= T75), Q144 (= Q144), F145 (= F145), Q229 (= Q221), G230 (= G222), I231 (= I223), A233 (= A225)

P0ABK5 Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; S-carboxymethylcysteine synthase; Sulfate starvation-induced protein 5; SSI5; EC 2.5.1.47; EC 4.5.1.5 from Escherichia coli (strain K12) (see 5 papers)
58% identity, 99% coverage: 2:307/308 of query aligns to 3:314/323 of P0ABK5

query
sites
P0ABK5

N

K

V

I

Y

F

E

E

S

D

I

N

T

S

E

L

T

T

V

I

G

G

R

H

T

T

P

P

L

L

V

V

R

R

I

L

R

N

R

R

M

I

A

G

D

N

H

-

S

-

Y

-

A

G

D

R

V

I

Y

L

A

A

K

K

L

V

E

E

S

S

F

R

N

N

P

P

L

S

S

F

S

S

V

V

K
|
K

D

C

R

R

V

I

G

G

V

A

A

N

M

M

I

I

E

W

D

D

A

A

E

E

R

K

N

R

G

G

L

V

I

L

G

K

P

P

E

G

T

V

T

E

I

L

I

V

E

E

P

P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

A

L

L

A

A

F

Y

S

V

C

A

A

A

S

A

R

R

G

G

Y

Y

R

K

L

L

I

T

L

L

T

T

M

M

P

P

D

E

T

T

M

M

S

S

V

I

E

E

R

R

R

K

L

L

K

K

I

A

L

L

G

G

A

A

E

N

L

L

V

V

L

L

T

T

E

E

G

G

A

A

A

K

G

G

M

M

K

K

G

G

A

A

I

I

A

Q

E

K

A

A

E

E

R

E

L

I

V

V

D

A

S

S

I

N

P

P

H

E

S

K

-

Y

L

L

M

L

L

L

Q

Q

F

F

N

S

N

N

P

P

A

A

N

N

P

P

E

E

T

I

H

H

R

E

R

K

T

T

A

G

R

P

E

E

I

I

W

W

D

E

D

D

T

T

D

D

G

G

Q

Q

V

V

D

D

I

V

F

F

I

I

A

A

G

G

V

V

G

G

T

T

G

G

T

T

I

L

T

T

G

G

V

V

G

S

S

R

V

Y

L

I

R

K

E

G

L

T

K

K

P

G

G

K

V

T

H

D

I

L

-

I

-

S

V

V

A

A

V

V

E

E

P

P

D

T

D

D

S

S

P

P

V

V

I

I

S

A

-

Q

-

A

-

L

G

A

G

G

E

E

-

E

-

I

-

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

G

A

N

N

L

L

D

D

P

L

S

K

V

L

I

V

D

D

E

K

I

V

I

I

T

G

V

I

S

T

N

N

Q

E

D

E

A

A

A

I

E

S

T

T

A

A

R

R

K

R

L

L

A

M

R

E

K

E

E

E

G

G

I

I

L

L

C

A

G

G

I

I

S

S

G

G

A

A

M

V

W

A

A

A

M

L

S

K

V

L

A

Q

A

E

R

D

P

E

A

S

M

F

E

T

G

N

K

K

N

N

I

I

V

V

L

I

L

L

P

P

D

S

T

S

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

W

A

L

L

F

F

A

A

D

D

K42 (= K44) modified: N6-(pyridoxal phosphate)lysine; mutation to A: Still stimulates tRNase activity of CdiA-CT in vitro and in vivo.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1d6sA Crystal structure of the k41a mutant of o-acetylserine sulfhydrylase complexed in external aldimine linkage with methionine (see paper)
58% identity, 99% coverage: 2:307/308 of query aligns to 2:313/322 of 1d6sA

query
sites
1d6sA

N

K

V

I

Y

Y

E

E

S

D

I

N

T

S

E

L

T

T

V

I

G

G

R

H

T

T

P

P

L

L

V

V

R

R

I

L

R

N

R

R

M

I

A

G

D

N

H

-

S

-

Y

-

A

G

D

R

V

I

Y

L

A

A

K

K

L

V

E

E

S

S

F

R

N

N

P

P

L

S

S

F

S

S

V

V

K
x
A

D

C

R

R

V

I

G

G

V

A

A

N

M

M

I

I

E

W

D

D

A

A

E

E

R

K

N

R

G

G

L

V

I

L

G

K

P

P

E

G

T

V

T

E

I

L

I

V

E

E

P

P

T
|
T

S
x
N

G

G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

F

Y

S

V

C

A

A

A

S

A

R

R

G

G

Y

Y

R

K

L

L

I

T

L

L

T

T

M

M

P

P

D

E

T

T

M

M

S

S

V

I

E

E

R

R

R

K

L

L

K

K

I

A

L

L

G

G

A

A

E

N

L

L

V

V

L

L

T

T

E

E

G

G

A

A

A

K

G

G

M

M

K

K

G

G

A

A

I

I

A

Q

E

K

A

A

E

E

R

E

L

I

V

V

D

A

S

S

I

D

P

P

H

Q

S

K

-

Y

L

L

M

L

L

L

Q

Q

Q
|
Q

F
|
F

N

S

N

N

P

P

A

A

N

N

P

P

E

E

T

I

H

H

R

E

R

K

T

T

A

G

R

P

E

E

I

I

W

W

D

E

D

D

T

T

D

D

G

G

Q

Q

V

V

D

D

I

V

F

F

I

I

A

S

G

G

V

V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

L

T

T

G

G

V

V

G

T

S

R

V

Y

L

I

R

K

E

G

L

T

K

K

P

G

G

K

V

T

H

D

I

L

-

I

-

T

V

V

A

A

V

V

E

E

P

P

D

T

D

D

S

S

P

P

V

V

I

I

S

A

-

Q

-

A

-

L

G

A

G

G

E

E

-

E

-

I

-

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

G

G

N

N

L

L

D

D

P

L

S

K

V

L

I

I

D

D

E

K

I

V

T

G

V

I

S

T

N

N

Q

E

D

E

A

A

A

I

E

S

T

T

A

A

R

R

K

R

L

L

A

M

R

E

K

E

E

E

G

G

I

I

L

L

C

A

G

G

I

I

S
|
S

S

S

G

G

A

A

M

V

W

A

A

A

M

L

S

K

V

L

A

Q

A

E

R

D

P

E

A

S

M

F

E

T

G

N

K

K

N

N

I

I

V

V

L

I

L

L

P
|
P

D

S

T

S

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

W

A

L

L

F

F

A

A

D

D

active site: A41 (≠ K44), G228 (= G222)
binding methionine: T68 (= T71), N69 (≠ S72), N71 (= N74), T72 (= T75), Q142 (= Q144), F143 (= F145), G176 (= G178), G228 (= G222)
binding pyridoxal-5'-phosphate: N71 (= N74), G176 (= G178), T177 (= T179), G178 (= G180), T180 (= T182), G228 (= G222), S272 (= S266), P299 (= P293)

P47999 Cysteine synthase, chloroplastic/chromoplastic; At.OAS.7-4; Beta-substituted Ala synthase 2;1; ARAth-Bsas2;1; CSase B; AtCS-B; CS-B; O-acetylserine (thiol)-lyase; O-acetylserine sulfhydrylase; OAS-TL B; cpACS1; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
57% identity, 99% coverage: 1:305/308 of query aligns to 73:378/392 of P47999

query
sites
P47999

M

L

N

N

V

I

Y

A

E

D

S

N

I

A

T

A

E

Q

T

L

V

I

G

G

R

K

T

T

P

P

L

M

V

V

R

Y

I

L

R

N

M

V

A

V

D

K

H

G

S

C

Y

V

A

A

D

S

V

V

Y

A

A

A

K

K

L

L

E

E

S

I

F

M

N

E

P

P

L

C

S

C

S

S

V

V

K

K

D

D

R

R

V

I

G

G

V

Y

A

S

M

M

I

I

E

T

D

D

A

A

E

E

R

E

N

K

G

G

L

L

I

I

G

T

P

P

-

G

E

K

T

S

T

V

I

L

I

V

E

E

P

S

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

G

L

L

A

A

F

F

S

I

C

A

A

A

S

S

R

K

G

G

Y

Y

R

K

L

L

I

I

L

L

T

T

M

M

P

P

D

A

T

S

M

M

S

S

V

L

E

E

R

R

R

V

L

L

K

R

I

A

L

F

G

G

A

A

E

E

L

L

V

V

L

L

T

T

E

E

G

P

A

A

A

K

G

G

M

M

K

T

G

G

A

A

I

I

A

Q

E

K

A

A

E

E

R

E

L

I

V

L

D

K

S

K

I

T

P

P

H

N

S

S

L

Y

M

M

L

L

Q

Q

F

F

N

D

N

N

P

P

A

A

N

N

P

P

E

K

T

I

H

H

R

Y

R

E

T

T

A

G

R

P

E

E

I

I

W

W

D

E

D

D

T

T

D

R

G

G

Q

K

V

I

D

D

I

I

F

L

I

V

A

A

G

G

V

I

G

G

T

T

G

G

T

T

I

I

T

T

G

G

V

V

G

G

S

R

V

F

L

I

R

K

E

E

L

R

K

K

P

P

G

E

V

L

H

K

I

V

V

I

A

G

V

V

E

E

P

P

D

T

D

E

S

S

P

A

V

I

I

L

S

S

G

G

E

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

V

P

P

G

K

N

N

L

L

D

D

P

L

S

A

V

I

I

V

D

D

E

E

I

Y

I

I

T

A

V

I

S

S

N

S

Q

E

D

E

A

A

A

I

E

E

T

T

A

S

R

K

K

Q

L

L

A

A

R

L

K

Q

E

E

G

G

I

L

L

L

C

V

G

G

I

I

S

S

G

G

A

A

M

A

W

A

A

A

M

I

S

Q

V

V

A

A

A

K

R

R

P

P

A

E

M

N

E

A

G

G

K

K

N

L

I

I

V

A

V

V

L

V

L

F

P

P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

W

Q

L

L

F

F

Sites not aligning to the query:

61 modified: N-acetylalanine

1fcjA Crystal structure of oass complexed with chloride and sulfate (see paper)
57% identity, 96% coverage: 2:296/308 of query aligns to 2:302/302 of 1fcjA

query
sites
1fcjA

N

K

V

I

Y

Y

E

E

S

D

I

N

T

S

E

L

T

T

V

I

G

G

R

H

T

T

P

P

L

L

V

V

R

R

I

L

R

N

R

R

M

I

A

G

D

N

H

-

S

-

Y

-

A

G

D

R

V

I

Y

L

A

A

K

K

L

V

E

E

S

S

F

R

N

N

P

P

L

S

S

F

S

S

V

V

K
|
K

D

C

R

R

V

I

G

G

V

A

A

N

M

M

I

I

E

W

D

D

A

A

E

E

R

K

N

R

G

G

L

V

I

L

G

K

P

P

E

G

T

V

T

E

I

L

I

V

E

E

P

P

T

T

S

N

G
|
G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

F

Y

S

V

C

A

A

A

S

A

R

R

G

G

Y

Y

R

K

L

L

I

T

L

L

T

T

M

M

P

P

D

E

T

T

M

M

S

S

V

I

E

E

R

R

R

K

L

L

K

K

I

A

L

L

G

G

A

A

E

N

L

L

V

V

L

L

T

T

E

E

G

G

A

A

A

K

G

G

M

M

K

K

G

G

A

A

I

I

A

Q

E

K

A

A

E

E

R

E

L

I

V

V

D

A

S

S

I

D

P

P

H

Q

S

K

-

Y

L

L

M

L

L

L

Q

Q

Q
|
Q

F

F

N

S

N

N

P

P

A

A

N

N

P

P

E

E

T

I

H

H

R

E

R

K

T

T

A

G

R

P

E

E

I

I

W

W

D

E

D

D

T

T

D

D

G

G

Q

Q

V

V

D

D

I

V

F

F

I

I

A

S

G

G

V
|
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

L

T

T

G

G

V

V

G

T

S

R

V

Y

L

I

R

K

E

G

L

T

K

K

P

G

G

K

V

T

H

D

I

L

-

I

-

T

V

V

A

A

V

V

E

E

P

P

D

T

D

D

S

S

P

P

V

V

I

I

S

A

-

Q

-

A

-

L

G

A

G

G

E

E

-

E

-

I

-

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

G

G

N

N

L

L

D

D

P

L

S

K

V

L

I

I

D

D

E

K

I

V

T

G

V

I

S

T

N

N

Q

E

D

E

A

A

A

I

E

S

T

T

A

A

R

R

K

R

L

L

A

M

R

E

K

E

E

E

G

G

I

I

L

L

C

A

G

G

I

I

S
|
S

S

S

G

G

A

A

M

V

W

A

A

A

M

L

S

K

V

L

A

Q

A

E

R

D

P

E

A

S

M

F

E

T

G

N

K

K

N

N

I

I

V

V

L

I

L

L

P
|
P

D

S

T

S

G

G

active site: K41 (= K44), G228 (= G222), S272 (= S266)
binding pyridoxal-5'-phosphate: K41 (= K44), N71 (= N74), V175 (= V177), G176 (= G178), T177 (= T179), G178 (= G180), T180 (= T182), G228 (= G222), S272 (= S266), P299 (= P293)
binding sulfate ion: G70 (= G73), T72 (= T75), Q142 (= Q144)

7n2tA O-acetylserine sulfhydrylase from citrullus vulgaris in the internal aldimine state, with citrate bound (see paper)
56% identity, 98% coverage: 3:305/308 of query aligns to 3:306/309 of 7n2tA

query
sites
7n2tA

V

I

Y

A

E

K

S

D

I

V

T

T

E

E

T

L

V

I

G

G

R

N

T

T

P

P

L

L

V

V

R

Y

I

L

R

N

R

R

M

V

A

V

D

D

H

G

S

C

Y

V

A

A

D

R

V

V

Y

A

A

A

K

K

L

L

E

E

S

M

F

M

N

E

P

P

L

C

S

S

V

V

K

K

D

D

R

R

V

I

G

G

V

Y

A

S

M

M

I

I

E

S

D

D

A

A

E

E

R

N

N

K

G

G

L

L

I

I

G

T

P

P

-

G

E

E

T

S

T

V

I

L

I

I

E

E

P

P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

G

L

L

A

A

F

F

S

I

C

A

A

A