SitesBLAST

G3XD01 UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronate N-acetyltransferase; UDP-D-GlcNAc3NA N-acetyltransferase; UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronic acid 3-N-acetyltransferase; EC 2.3.1.201 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
32% identity, 41% coverage: 75:181/264 of query aligns to 21:150/191 of G3XD01

query
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G3XD01

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K136 (= K167) mutation to A: Reduction in catalytic activity.; mutation to R: No effect on catalytic activity.

4mzuF Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
33% identity, 39% coverage: 100:201/264 of query aligns to 58:153/294 of 4mzuF

query
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4mzuF

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binding coenzyme a: N81 (≠ G123), D82 (≠ T124), K83 (≠ S125), G100 (= G142), A101 (= A143), L106 (= L148), G118 (= G160), A119 (≠ S161), T124 (≠ V166), K125 (= K167), I134 (≠ V176), N136 (≠ I178), R139 (= R181)

Sites not aligning to the query:

binding thymine: 20, 21, 38
binding thymidine-5'-diphosphate: 189, 203, 205, 270

4mzuB Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
34% identity, 36% coverage: 100:193/264 of query aligns to 58:148/290 of 4mzuB

query
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4mzuB

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binding coenzyme a: F72 (≠ T114), T80 (≠ G122), N81 (≠ G123), D82 (≠ T124), K83 (≠ S125), P85 (= P128), G99 (= G142), A100 (= A143), G117 (= G160), A118 (≠ S161), T123 (≠ V166), K124 (= K167), I133 (≠ V176), R138 (= R181)
binding magnesium ion: G58 (= G100), N76 (≠ G118)

Sites not aligning to the query:

binding coenzyme a: 199
binding thymine: 20, 21, 38, 39
binding thymidine-5'-diphosphate: 171, 176, 189, 191, 202, 203, 270

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
30% identity, 41% coverage: 77:183/264 of query aligns to 70:182/203 of P07464

query
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P07464

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S71 (≠ W78) binding substrate
N85 (≠ D96) binding in other chain; binding in other chain
D93 (≠ G104) binding substrate
H115 (≠ W126) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (≠ A143) binding in other chain
A160 (≠ S161) binding in other chain
TK 165:166 (≠ VK 166:167) binding acetyl-CoA
R180 (= R181) binding acetyl-CoA

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial
17 binding substrate
183 binding in other chain

Query Sequence

>WP_012536274.1 NCBI__GCF_000021485.1:WP_012536274.1
MRGLVVAGANTGGVAMGGRWRSDLDAVFARDPAARTRLEVLLTYPGVHALFLHRIAHALW
RHRWRLLARSLAAVSRFWTGIEIHPGAQIGKGFFIDHGMGVVIGETAEIGDDCTLYHGVT
LGGTSWKPGKRHPTLGRGVIVGAGAKVLGPVVIGDGARIGSNAVVVKSVPEGATVVGIPG
RVVNKGEHTDNFEAYGLTGQMPDPVARAVECMLEHMHRQDAEIAQLRDGVQRLQPLETMM
PVEEIVCVLDDDAPEGEGTRAGNT

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory