SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012536327.1 NCBI__GCF_000021485.1:WP_012536327.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P17597 Acetolactate synthase, chloroplastic; AtALS; Acetohydroxy-acid synthase; Protein CHLORSULFURON RESISTANT 1; EC 2.2.1.6 from Arabidopsis thaliana (Mouse-ear cress) (see 8 papers)
46% identity, 96% coverage: 22:579/581 of query aligns to 99:661/670 of P17597

query
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P17597

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A122 (= A45) mutation to V: Reduced catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
M124 (≠ L47) mutation to E: Reduced catalytic activity. Resistant to imidazolinone herbicides and reduced sensitivity to sulfonylurea herbicides.; mutation to I: No effect on catalytic activity. Increased resistance to imidazolinone herbicides.
E144 (= E67) binding thiamine diphosphate
S186 (= S109) binding FAD
P197 (= P120) mutation to S: In csr1-1/GH50; resistant to sulfonylurea but not to imidazolinone herbicides.
R199 (≠ A122) mutation R->A,E: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
Q207 (= Q130) binding thiamine diphosphate
K220 (= K143) binding (R)-imazaquin
R246 (= R169) binding (R)-imazaquin; binding FAD
K256 (= K179) binding chlorimuron-ethyl
G308 (= G229) binding FAD
TL 331:332 (= TL 254:255) binding FAD
C340 (≠ A263) modified: Cysteine sulfinic acid (-SO2H)
LGMH 349:352 (= LGMH 272:275) binding FAD
GVRFD 371:375 (≠ GARFD 294:298) binding FAD
DR 376:377 (= DR 299:300) binding chlorimuron-ethyl
DI 395:396 (≠ DV 318:319) binding FAD
DV 414:415 (≠ DL 337:338) binding FAD
QH 487:488 (= QH 411:412) binding thiamine diphosphate
GG 508:509 (= GG 432:433) binding FAD
GAM 511:513 (≠ GTM 435:437) binding thiamine diphosphate
D538 (= D462) binding Mg(2+)
DGS 538:540 (= DGS 462:464) binding thiamine diphosphate
N565 (= N489) binding Mg(2+)
NQHLGM 565:570 (≠ NHYLGM 489:494) binding thiamine diphosphate
H567 (≠ Y491) binding Mg(2+)
W574 (= W498) binding chlorimuron-ethyl; mutation to L: Increased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.; mutation to S: Slightly decreased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.
S653 (≠ A571) binding chlorimuron-ethyl; mutation to A: No effect on catalytic activity or sensitivity to herbicides.; mutation to F: No effect on catalytic activity. Resistant to imidazolinone herbicides and also slightly sulfonylurea-resistant.; mutation to N: In csr1-2/GH90; no effect on catalytic activity. Resistant to imidazolinone but not to sulfonylurea herbicides.; mutation to T: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.

8et4A Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with amidosulfuron (see paper)
46% identity, 96% coverage: 22:579/581 of query aligns to 14:576/582 of 8et4A

query
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8et4A

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binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V409), G401 (= G410), Q402 (= Q411), H403 (= H412), G426 (= G435), M428 (= M437), G452 (= G461), D453 (= D462), G454 (= G463), S455 (= S464), M458 (= M467), N480 (= N489), H482 (≠ Y491), L483 (= L492), G484 (= G493), M485 (= M494), V486 (= V495)
binding flavin-adenine dinucleotide: R161 (= R169), G222 (= G228), G223 (= G229), G224 (= G230), T246 (= T254), L247 (= L255), M248 (= M256), L264 (= L272), M266 (= M274), H267 (= H275), G286 (= G294), V287 (≠ A295), R288 (= R296), D290 (= D298), R292 (= R300), V293 (= V301), D310 (= D318), I311 (≠ V319), D329 (= D337), V330 (≠ L338), M405 (= M414), G423 (= G432)
binding magnesium ion: F370 (≠ Y379), D453 (= D462), M458 (= M467), Q461 (= Q470), N480 (= N489), H482 (≠ Y491), K533 (≠ P535)
binding N-{[(4,6-dimethoxypyrimidin-2-yl)carbamoyl]sulfamoyl}-N-methylmethanesulfonamide: M266 (= M274), R292 (= R300), M485 (= M494), W489 (= W498), S568 (≠ A571)

5wj1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a triazolopyrimidine herbicide, penoxsulam (see paper)
46% identity, 96% coverage: 22:579/581 of query aligns to 14:576/582 of 5wj1A

query
sites
5wj1A

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active site: Y33 (= Y41), G35 (= G43), G36 (= G44), A37 (= A45), S38 (≠ V46), E59 (= E67), T82 (= T90), F121 (= F129), Q122 (= Q130), E123 (= E131), K171 (= K179), M266 (= M274), V293 (= V301), V400 (= V409), G426 (= G435), M428 (= M437), D453 (= D462), N480 (= N489), H482 (≠ Y491), L483 (= L492), M485 (= M494), V486 (= V495), W489 (= W498), H558 (≠ P561)
binding flavin-adenine dinucleotide: R161 (= R169), G222 (= G228), G223 (= G229), G224 (= G230), T246 (= T254), L247 (= L255), M248 (= M256), M263 (= M271), L264 (= L272), G286 (= G294), R288 (= R296), V293 (= V301), D310 (= D318), I311 (≠ V319), D329 (= D337), V330 (≠ L338), M405 (= M414), G423 (= G432), G424 (= G433)
binding magnesium ion: D453 (= D462), N480 (= N489), H482 (≠ Y491)
binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: M266 (= M274), D291 (= D299), R292 (= R300), M485 (= M494), W489 (= W498), S568 (≠ A571)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V409), G401 (= G410), Q402 (= Q411), H403 (= H412), M428 (= M437), D453 (= D462), G454 (= G463), S455 (= S464), M458 (= M467), N480 (= N489), H482 (≠ Y491), L483 (= L492), G484 (= G493), M485 (= M494), V486 (= V495)

5k6tA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, propoxycarbazone-sodium (see paper)
46% identity, 96% coverage: 22:579/581 of query aligns to 14:576/582 of 5k6tA

query
sites
5k6tA

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N

E

D

M

V

I

I

active site: Y33 (= Y41), G35 (= G43), G36 (= G44), A37 (= A45), S38 (≠ V46), E59 (= E67), T82 (= T90), F121 (= F129), Q122 (= Q130), E123 (= E131), K171 (= K179), M266 (= M274), V293 (= V301), V400 (= V409), G426 (= G435), M428 (= M437), D453 (= D462), N480 (= N489), H482 (≠ Y491), L483 (= L492), M485 (= M494), V486 (= V495), W489 (= W498), H558 (≠ P561)
binding methyl 2-[(4-methyl-5-oxidanylidene-3-propoxy-1,2,4-triazol-1-yl)carbonylsulfamoyl]benzoate: H267 (= H275), R292 (= R300), M485 (= M494), W489 (= W498), S568 (≠ A571)
binding flavin-adenine dinucleotide: R161 (= R169), G222 (= G228), G223 (= G229), G224 (= G230), T246 (= T254), L247 (= L255), M248 (= M256), L264 (= L272), G286 (= G294), R288 (= R296), D290 (= D298), R292 (= R300), V293 (= V301), D310 (= D318), I311 (≠ V319), D329 (= D337), V330 (≠ L338), Q404 (= Q413), M405 (= M414), G423 (= G432)
binding magnesium ion: D453 (= D462), N480 (= N489), H482 (≠ Y491)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (= V409), G401 (= G410), Q402 (= Q411), H403 (= H412), G426 (= G435), M428 (= M437), G452 (= G461), G454 (= G463), S455 (= S464), N480 (= N489), H482 (≠ Y491), L483 (= L492), G484 (= G493)

5k6rA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, thiencarbazone-methyl (see paper)
46% identity, 96% coverage: 22:579/581 of query aligns to 14:576/582 of 5k6rA

query
sites
5k6rA

G

G

A

A

E

D

I

I

V

L

V

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E

A

A

L

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x
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R

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N
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N

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x
H

L
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L

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M
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M

V
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Q

Q

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W

Q

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E

D

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F

F

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Y

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K

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A

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N

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M

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G

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-

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D

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L

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G

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V

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A

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T

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D

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L

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P

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A

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Q

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A

M

L

L

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D

L

T

K

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D

G

R

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M

Y

V

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M

L

D

D

F

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I

V

C

D

P

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x
H

T

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N

H

V

V

Y

L

P

P

M

M

V

I

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P

A
x
S

G

G

A

G

A

T

L

F

S

N

E

D

M

V

I

I

active site: Y33 (= Y41), G35 (= G43), G36 (= G44), A37 (= A45), S38 (≠ V46), E59 (= E67), T82 (= T90), F121 (= F129), Q122 (= Q130), E123 (= E131), K171 (= K179), M266 (= M274), V293 (= V301), V400 (= V409), G426 (= G435), M428 (= M437), D453 (= D462), N480 (= N489), H482 (≠ Y491), L483 (= L492), M485 (= M494), V486 (= V495), W489 (= W498), H558 (≠ P561)
binding methyl 4-[(3-methoxy-4-methyl-5-oxidanylidene-1,2,4-triazol-1-yl)carbonylsulfamoyl]-5-methyl-thiophene-3-carboxylate: R292 (= R300), W489 (= W498), S568 (≠ A571)
binding flavin-adenine dinucleotide: R161 (= R169), G222 (= G228), G223 (= G229), G224 (= G230), T246 (= T254), L247 (= L255), M248 (= M256), L264 (= L272), M266 (= M274), G286 (= G294), R288 (= R296), R292 (= R300), V293 (= V301), D310 (= D318), I311 (≠ V319), G328 (= G336), D329 (= D337), V330 (≠ L338), M405 (= M414), G423 (= G432)
binding magnesium ion: D453 (= D462), N480 (= N489), H482 (≠ Y491)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (= V409), G401 (= G410), Q402 (= Q411), H403 (= H412), G426 (= G435), M428 (= M437), D453 (= D462), G454 (= G463), S455 (= S464), M458 (= M467), N480 (= N489), H482 (≠ Y491), L483 (= L492), G484 (= G493), M485 (= M494), V486 (= V495)

1z8nA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with an imidazolinone herbicide, imazaquin (see paper)
46% identity, 96% coverage: 22:579/581 of query aligns to 14:576/582 of 1z8nA

query
sites
1z8nA

G

G

A

A

E

D

I

I

V

L

V

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A

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Y

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A
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Y

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G

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K

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G

G

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V

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L

I

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T

S

S

G

G

P

P

G

G

A

A

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N

N

A

L

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V

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G

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D

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V

Q

I

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x
H

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E

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H

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G
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G

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G

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I

active site: Y33 (= Y41), G35 (= G43), G36 (= G44), A37 (= A45), S38 (≠ V46), E59 (= E67), T82 (= T90), F121 (= F129), Q122 (= Q130), E123 (= E131), K171 (= K179), M266 (= M274), V293 (= V301), V400 (= V409), G426 (= G435), M428 (= M437), D453 (= D462), N480 (= N489), H482 (≠ Y491), L483 (= L492), M485 (= M494), V486 (= V495), W489 (= W498), H558 (≠ P561)
binding 2-(4-isopropyl-4-methyl-5-oxo-4,5-dihydro-1h-imidazol-2-yl)quinoline-3-carboxylic acid: K135 (= K143), R161 (= R169), Y191 (= Y199), R194 (vs. gap), D291 (= D299), R292 (= R300), D312 (= D320), W489 (= W498), G569 (= G572)
binding flavin-adenine dinucleotide: R161 (= R169), G222 (= G228), G224 (= G230), T246 (= T254), L247 (= L255), M248 (= M256), L264 (= L272), G265 (= G273), M266 (= M274), H267 (= H275), G286 (= G294), V287 (≠ A295), R288 (= R296), D290 (= D298), R292 (= R300), V293 (= V301), D310 (= D318), I311 (≠ V319), D329 (= D337), V330 (≠ L338), M405 (= M414), G423 (= G432), G424 (= G433)
binding magnesium ion: D453 (= D462), N480 (= N489)
binding thiamine diphosphate: V400 (= V409), G401 (= G410), Q402 (= Q411), H403 (= H412), G426 (= G435), M428 (= M437), G452 (= G461), G454 (= G463), S455 (= S464), N480 (= N489), H482 (≠ Y491), L483 (= L492), G484 (= G493), M485 (= M494), V486 (= V495)

1yi1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, tribenuron methyl (see paper)
46% identity, 96% coverage: 22:579/581 of query aligns to 14:576/582 of 1yi1A

query
sites
1yi1A

G

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