SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012536755.1 NCBI__GCF_000021485.1:WP_012536755.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 12 hits to proteins with known functional sites (download)

P0A7B5 Glutamate 5-kinase; Gamma-glutamyl kinase; GK; EC 2.7.2.11 from Escherichia coli (strain K12) (see paper)
41% identity, 93% coverage: 24:393/399 of query aligns to 2:365/367 of P0A7B5

query
sites
P0A7B5

T

S

D

D

A

S

K

Q

R

T

W

L

V

V

I

V

K

K

I

L

G

G

S

T

S

S

L

V

L

L

T

T

N

G

D

G

G

S

Q

R

H

R

L

L

D

N

I

R

T

A

A

H

I

I

R

V

A

E

W

L

M

V

Q

R

Q

Q

I

C

L

A

L

Q

L

L

H

H

Q

A

R

A

G

G

V

H

E

R

V

I

V

V

L

I

V

V

S

T

S
|
S

G

G

S

A

V

I

S

A

A

A

G

G

M

R

Q

E

R

H

L

L

G

G

W

Y

T

P

D

E

R

L

P

P

V

A

S

T

L

I

K

A

A

S

R

K

Q

Q

A

L

A

L

A

A

S

A

V

V

G

G

Q

Q

S

S

A

R

L

L

I

I

H

Q

T

L

Y

W

E

E

K

Q

M

L

L

F

Q

S

E

I

C

Y

A

G

Q

-

P

-

E

-

N

-

D

-

R

-

L

I

Y

H

C

V

G

G

Q

Q

V

M

L

L

L

L

T

T

H

R

D

A

D

D

L

M

R

E

T

D

R

R

R

E

R

R

Y

F

L

L

N

N

A

A

R

R

S

D

T

T

L

L

R

R

T

A

L

L

L

L

D

D

M

N

N

N

V

I

L

V

P

P

I

V

I

I

N

N

E

E

N

N

D
|
D

V

A

V

V

S

A

Y

T

S

A

A

E

I

I

N

K

L

V

G

G

D

D

N
|
N

D

D

T

N

L

L

A

S

A

A

L

L

V

A

S

A

N

I

L

L

L

A

D

G

A

A

D

D

I

K

L

L

V

L

I

L

L

L

T

T

D

D

Q

Q

T

K

G

G

L

L

F

Y

D

T

A

A

D

D

P

P

R

R

S

S

H

N

P

P

E

Q

A

A

T

E

L

L

L

I

T

K

E

D

V

V

G

Y

A

G

G

I

D

D

P

D

M

A

L

L

E

R

R

A

I

I

A

A

G

G

G

D

G

S

G

V

S

S

R

G

M

L

G

G

T

T

G

G

G

G

M

M

L

S

A

T

K

K

V

L

R

Q

A

A

A

A

A

D

R

V

A

A

A

C

R

R

S

A

G

G

T

I

S

D

T

T

I

I

I

I

A

A

D

A

G

G

R

S

R

K

P

P

E

G

V

V

L

I

P

G

R

D

L

V

H

M

Q

E

G

G

E

I

A

S

I

V

G

G

T

T

F

L

V

F

H

H

A

A

R

Q

L

A

P

T

K

P

L

L

A

E

A

N

R

R

K

K

R

R

W

W

L

I

A

F

G

G

H

A

L

P

R

P

S

A

H

-

G

G

V

E

L

I

H

T

L

V

D

D

Q

E

G

G

A

A

A

T

R

A

A

A

I

I

L

L

E

E

E

R

G

G

S

S

S

S

L

L

L

L

P

P

V

K

G

G

V

I

I

K

A

S

V

V

E

T

G

G

T

N

F

F

R

S

R

R

G

G

D

E

L

V

V

I

V

R

C

I

K

C

D

N

P

L

L

E

G

G

R

R

D

D

I

I

A

A

R

H

G

G

L

V

I

S

N

R

L

Y

D

N

A

S

P

D

V

A

A

L

A

R

R

R

C

I

C

A

R

G

K

H

Q

H

S

S

H

Q

E

E

L

I

-

D

A

A

D

I

D

L

P

G

E

Y

V

E

L

Y

E

G

D

P

V

V

L

A

I

V

H

H

R

R

D

D

N

D

L

M

V

I

S50 (= S72) binding substrate
D137 (= D165) binding substrate
N149 (= N177) binding substrate

2j5tD Glutamate 5-kinase from escherichia coli complexed with glutamate (see paper)
42% identity, 92% coverage: 25:393/399 of query aligns to 1:363/365 of 2j5tD

query
sites
2j5tD

D

D

A

S

K

Q

R

T

W

L

V

V

I

V

K

K

I

L

G

G

S

T

S

S

L

V

L

L

T

T

N

G

D

G

G

S

Q

R

H

R

L

L

D

N

I

R

T

A

A

H

I

I

R

V

A

E

W

L

M

V

Q

R

Q

Q

I

C

L

A

L

Q

L

L

H

H

Q

A

R

A

G

G

V

H

E

R

V

I

V

V

L

I

V

V

S

T

S
|
S

G
|
G

S
x
A

V

I

S

A

A

A

G

G

M

R

Q

E

R

H

L

L

G

G

W

Y

T

P

D

E

R

L

P

P

V

A

S

T

L

I

K

A

A

S

R

K

Q

Q

A

L

A

L

A

A

S

A

V

V

G

G

Q

Q

S

S

A

R

L

L

I

I

H

Q

T

L

Y

W

E

E

K

Q

M

L

L

F

Q

S

E

I

C

Y

A

G

Q

-

P

-

E

-

N

-

D

-

R

-

L

I

Y

H

C

V

G

G

Q

Q

V

M

L

L

L

L

T

T

H

R

D

A

D

D

L

M

R

E

T

D

R

R

R

E

R

R

Y

F

L

L

N

N

A

A

R

R

S

D

T

T

L

L

R

R

T

A

L

L

L

L

D

D

M

N

N

N

V

V

L

V

P

P

I

V

I

I

N
|
N

E

E

N

N

D
|
D

V

A

V

V

S

A

Y

T

S

A

A

E

I

I

N

K

L

V

G

G

D
|
D

N
|
N

D

D

T

N

L

L

A

S

A

A

L

L

V

A

S

A

N

I

L

L

L

A

D

G

A

A

D

D

I

K

L

L

V

L

I

L

L

L

T

T

D

D

Q

Q

T

K

G

G

L

L

F

Y

D

T

A

A

D

D

P

P

R

R

S

S

H

N

P

P

E

Q

A

A

T

E

L

L

L

I

T

K

E

D

V

V

G

Y

A

G

G

I

D

D

P

D

M

A

L

L

E

R

R

A

I

I

A

A

G

G

G

D

G

S

G

V

S

S

R

G

M

L

G

G

T

T

G

G

G

G

M

M

L

S

A

T

K

K

V

L

R

Q

A

A

A

A

A

D

R

V

A

A

A

C

R

R

S

A

G

G

T

I

S

D

T

T

I

I

I

I

A

A

D

A

G

G

R

S

R

K

P

P

E

G

V

V

L

I

P

G

R

D

L

V

H

M

Q

E

G

G

E

I

A

S

I

V

G

G

T

T

F

L

V

F

H

H

A

A

R

Q

L

A

P

T

K

P

L

L

A

E

A

N

R

R

K

K

R

R

W

W

L

I

A

F

G

G

H

A

L

P

R

P

S

A

H

-

G

G

V

E

L

I

H

T

L

V

D

D

Q

E

G

G

A
|
A

A

T

R

A

A
|
A

I

I

L

L

E

E

E

R

G

G

S

S

S

S

L

L

L

L

P

P

V

K

G

G

V

I

I

K

A

S

V

V

E

T

G

G

T

N

F

F

R

S

R

R

G

G

D

E

L

V

V

I

V

R

C

I

K

C

D

N

P

L

L

E

G

G

R

R

D

D

I

I

A

A

R

H

G

G

L

V

I

S

N

R

L

Y

D

N

A

S

P

D

V

A

A

L

A

R

R

R

C

I

C

A

R

G

K

H

Q

H

S

S

H

Q

E

E

L

I

-

D

A

A

D

I

D

L

P

G

E

Y

V

E

L

Y

E

G

D

P

V

V

L

A

I

V

H

H

R

R

D

D

N

D

L

M

V

I

binding glutamic acid: S48 (= S72), G49 (= G73), A50 (≠ S74), N132 (= N162), D135 (= D165), D146 (= D176), N147 (= N177), A282 (= A313), A285 (= A316)

2j5vB Glutamate 5-kinase from escherichia coli complexed with glutamyl-5- phosphate and pyroglutamic acid (see paper)
36% identity, 92% coverage: 25:393/399 of query aligns to 1:323/325 of 2j5vB

query
sites
2j5vB

D

D

A

S

K

Q

R

T

W

L

V

V

I

V

K
|
K

I

L

G

G

S
x
T

S

S

L

V

L

L

T

T

N

G

D

G

G

S

Q

R

H

R

L

L

D

N

I

R

T

A

A

H

I

I

R

V

A

E

W

L

M

V

Q

R

Q

Q

I

C

L

A

L

Q

L

L

H

H

Q

A

R

A

G

G

V

H

E

R

V

I

V

V

L

I

V

V

S

T

S
|
S

G
|
G

S
x
A

V
x
I

S
x
A

A

A

G

G

M

R

Q

E

R

H

L

L

G

G

W

Y

T

P

D

E

R

L

P

P

V

A

S

T

L

I

K

A

A

S

R

K

Q

Q

A

L

A

L

A

A

S

A

V

V

G

G

Q

Q

S

S

A

R

L

L

I

I

H

Q

T

L

Y

W

E

E

K

Q

M

L

L

F

Q

S

E

I

C

Y

A

G

Q

-

P

-

E

-

N

-

D

-

R

-

L

I

Y

H

C

V

G

G

Q

Q

V

M

L

L

L

L

T

T

H

R

D

A

D

D

L

M

R

E

T

D

R

R

R

E

R

R

Y

F

L

L

N

N

A

A

R

R

S

D

T

T

L

L

R

R

T

A

L

L

L

L

D

D

M

N

N

N

V

V

L

V

P

P

I

V

I

I

N

N

E

E

N

N

D
|
D

V

A

V

V

S

A

Y

T

S

A

A

E

I

I

N

K

L

V

G
|
G

D
|
D

N
|
N

D

D

T

N

L

L

A

S

A

A

L

L

V

A

S

A

N

I

L

L

L

A

D

G

A

A

D

D

I

K

L

L

V

L

I

L

L

L

T

T

D

D

Q

Q

T

-

G

-

L

-

F

-

D

-

A

-

D

-

P

-

R

-

S

-

H

-

P

-

E

-

A

-

T

-

L

-

L

-

T

-

E

-

V

-

G

-

A

-

G

-

D

-

P

-

M

-

L

-

E

-

R

-

I

-

A

-

G

-

G

-

G

-

G

-

S

-

R

-

M

-

G

-

T

-

G

G

G

G

M

M

L

S

A

T

K

K

V

L

R

Q

A

A

A

A

A

D

R

V

A

A

A

C

R

R

S

A

G

G

T

I

S

D

T

T

I

I

I

I

A

A

D

A

G

G

R

S

R

K

P

P

E

G

V

V

L

I

P

G

R

D

L

V

H

M

Q

E

G

G

E

I

A

S

I

V

G

G

T

T

F

L

V

F

H

H

A

A

R

Q

L

A

P

T

K

P

L

L

A

E

A

N

R

R

K

K

R

R

W

W

L

I

A

F

G

G

H

A

L

P

R

P

S

A

H

-

G

G

V

E

L

I

H

T

L

V

D

D

Q

E

G

G

A

A

A

T

R

A

A

A

I

I

L

L

E

E

E

R

G

G

S

S

S

S

L

L

L

L

P

P

V

K

G

G

V

I

I

K

A

S

V

V

E

T

G

G

T

N

F

F

R

S

R

R

G

G

D

E

L

V

V

I

V

R

C

I

K

C

D

N

P

L

L

E

G

G

R

R

D

D

I

I

A

A

R

H

G

G

L

V

I

S

N

R

L

Y

D

N

A

S

P

D

V

A

A

L

A

R

R

R

C

I

C

A

R

G

K

H

Q

H

S

S

H

Q

E

E

L

I

-

D

A

A

D

I

D

L

P

G

E

Y

V

E

L

Y

E

G

D

P

V

V

L

A

I

V

H

H

R

R

D

D

N

D

L

M

V

I

binding pyroglutamic acid: T11 (≠ S35), G49 (= G73), A50 (≠ S74), I51 (≠ V75), A52 (≠ S76), D135 (= D165)
binding gamma-glutamyl phosphate: K8 (= K32), S48 (= S72), D135 (= D165), G145 (= G175), D146 (= D176), N147 (= N177)

2j5vA Glutamate 5-kinase from escherichia coli complexed with glutamyl-5- phosphate and pyroglutamic acid (see paper)
36% identity, 92% coverage: 25:393/399 of query aligns to 1:321/323 of 2j5vA

query
sites
2j5vA

D

D

A

S

K

Q

R

T

W

L

V

V

I

V

K
|
K

I

L

G
|
G

S
x
T

S

S

L

V

L

L

T

T

N

G

D

G

G

S

Q

R

H

R

L

L

D

N

I

R

T

A

A

H

I

I

R

V

A

E

W

L

M

V

Q

R

Q

Q

I

C

L

A

L

Q

L

L

H

H

Q

A

R

A

G

G

V

H

E

R

V

I

V

V

L

I

V

V

S

T

S
|
S

G
|
G

S
x
A

V
x
I

S

A

A

A

G

G

M

R

Q

E

R

H

L

L

G

G

W

Y

T

P

D

E

R

L

P

P

V

A

S

T

L

I

K

A

A

S

R

K

Q

Q

A

L

A

L

A

A

S

A

V

V

G

G

Q

Q

S

S

A

R

L

L

I

I

H

Q

T

L

Y

W

E

E

K

Q

M

L

L

F

Q

S

E

I

C

Y

A

G

Q

-

P

-

E

-

N

-

D

-

R

-

L

I

Y

H

C

V

G

G

Q

Q

V

M

L

L

L

L

T

T

H

R

D

A

D

D

L

M

R

E

T

D

R

R

R

E

R

R

Y

F

L

L

N

N

A

A

R

R

S

D

T

T

L

L

R

R

T

A

L

L

L

L

D

D

M

N

N

N

V

V

L

V

P

P

I

V

I

I

N

N

E

E

N

N

D
|
D

V

A

V

V

S

A

Y

T

S

A

A

E

I

I

N

K

L

V

G

G

D
|
D

N
|
N

D

D

T

N

L

L

A

S

A

A

L

L

V

A

S

A

N

I

L

L

L

A

D

G

A

A

D

D

I

K

L

L

V

L

I

L

L
|
L

T

T

D

D

Q

Q

T

-

G

-

L

-

F

-

D

-

A

-

D

-

P

-

R

-

S

-

H

-

P

-

E

-

A

-

T

-

L

-

L

-

T

-

E

-

V

-

G

-

A

-

G

-

D

-

P

-

M

-

L

-

E

-

R

-

I

-

A

-

G

-

G

-

G

-

G

-

S

-

R

-

M

-

G

-

T

-

G

-

G

-

M

M

L

S

A

T

K

K

V

L

R

Q

A

A

A

A

A

D

R

V

A

A

A

C

R

R

S

A

G

G

T

I

S

D

T

T

I

I

I

I

A

A

D

A

G

G

R

S

R

K

P

P

E

G

V

V

L

I

P

G

R

D

L

V

H

M

Q

E

G

G

E

I

A

S

I

V

G

G

T

T

F

L

V

F

H

H

A

A

R

Q

L

A

P

T

K

P

L

L

A

E

A

N

R

R

K

K

R

R

W

W

L

I

A

F

G

G

H

A

L

P

R

P

S

A

H

-

G

G

V

E

L

I

H

T

L

V

D

D

Q

E

G

G

A

A

A

T

R

A

A

A

I

I

L

L

E

E

E

R

G

G

S

S

S

S

L

L

L

L

P

P

V

K

G

G

V

I

I

K

A

S

V

V

E

T

G

G

T

N

F

F

R

S

R

R

G

G

D

E

L

V

V

I

V

R

C

I

K

C

D

N

P

L

L

E

G

G

R

R

D

D

I

I

A

A

R

H

G

G

L

V

I

S

N

R

L

Y

D

N

A

S

P

D

V

A

A

L

A

R

R

R

C

I

C

A

R

G

K

H

Q

H

S

S

H

Q

E

E

L

I

-

D

A

A

D

I

D

L

P

G

E

Y

V

E

L

Y

E

G

D

P

V

V

L

A

I

V

H

H

R

R

D

D

N

D

L

M

V

I

binding magnesium ion: K8 (= K32), G10 (= G34), L166 (= L196)
binding pyroglutamic acid: T11 (≠ S35), S48 (= S72), G49 (= G73), A50 (≠ S74), I51 (≠ V75)
binding gamma-glutamyl phosphate: K8 (= K32), G10 (= G34), S48 (= S72), D135 (= D165), D146 (= D176), N147 (= N177)

2akoA Crystal structure of glutamate 5-kinase from campylobacter jejuni
31% identity, 60% coverage: 27:264/399 of query aligns to 1:219/241 of 2akoA

query
sites
2akoA

K

K

R

R

W

I

V

V

I

V

K

K

I

V

G

G

S

S

S
x
H

L

V

L

I

T

S

N

E

D

E

G

N

Q

T

H

-

L

L

D

S

I

F

T

E

A

R

I

L

R

K

A

N

W

L

M

V

Q

A

Q

F

I

L

L

A

L

K

L

L

H

M

Q

E

R

K

G

-

V

Y

E

E

V

V

V

I

L

L

V

V

S

T

S

S

G

A

S

A

V

I

S

S

A

A

G

G

M

H

Q

T

R

K

L

L

G

D

W

-

T

I

D

D

R

R

P

K

V

-

S

N

L

L

K

I

A

N

R

K

Q

Q

A

V

A

L

A

A

S

A

V

I

G

G

Q

Q

S

P

A

F

L

L

I

I

H

S

T

V

Y

Y

E

N

K

E

M

L

L

L

Q

A

E

K

C

F

A

N

Q

K

P

-

E

-

N

-

D

-

R

-

L

-

Y

L

C

G

G

G

Q

Q

V

I

L

L

L

L

T

T

H

G

D

K

D

D

L

F

R

D

T

S

R

R

R

K

R

A

Y

T

L

K

N

H

A

A

R

K

S

N

T

A

L

I

R

D

T

M

L

M

L

I

D

N

M

L

N

G

V

I

L

L

P

P

I

I

I

I

N

N

E

E

N

N

D

D

V

A

V

T

S

A

Y

I

S

E

A

E

I

I

N

V

L

F

G

G

D

D

N

N

D

D

T

S

L

L

A

S

A

A

L

Y

V

A

S

T

N

H

L

F

L

F

D

D

A

A

D

D

I

L

L

L

V

V

I

I

L

L

T
x
S

D
|
D

Q
x
I

T

D

G

G

L
x
F

F
x
Y

D

D

A

K

D
x
N

P
|
P

R

S

S

E

H

F

P

S

E

D

A

A

T

-

L

-

L

-

T

-

E

-

V

-

G

-

A

-

G

-

D

-

P

K

M

R

L

L

E

E

R

K

I

I

A

T

G

H

G

I

G

K

G

E

S

E

R

W

M

L

-

H

G

G

T
|
T

G

G

G
|
G

M

I

L

V

A

T

K
|
K

V

L

R

K

A

A

A

A

A

K

R

F

A

L

A

L

R

E

S

H

G

N

T

K

S

K

T

M

I

F

I

L

A

A

D

S

G

G

binding adenosine-5'-diphosphate: H10 (≠ S36), S161 (≠ T197), D162 (= D198), I163 (≠ Q199), F166 (≠ L202), Y167 (≠ F203), N170 (≠ D206), P171 (= P207), T194 (= T239), G196 (= G241), K200 (= K245)

7wx3B Gk domain of drosophila p5cs filament with glutamate, atp, and NADPH (see paper)
30% identity, 67% coverage: 16:283/399 of query aligns to 3:256/258 of 7wx3B

query
sites
7wx3B

W

F

L

T

N

E

R

R

S

S

N

Q

L

L

Q

K

T

Y

D

-

A

A

K

R

R

R

W

L

V

V

I

V

K

K

I

L

G

G

S

S

S

A

L

V

L

I

T

T

N

R

D

E

G

D

Q

N

H

H

-

G

L

L

D

A

I

L

T

G

A

R

I

L

R

A

A

S

W

I

M

V

Q

E

Q

Q

I

V

L

A

L

E

L

C

H

H

Q

L

R

E

G

G

V

R

E

E

V

V

V

M

L

M

V

V

S

T

S
|
S

G

G

S
x
A

V

V

S

A

A

F

G

G

M

K

Q

Q

R

K

L

L

G

A

W

-

T

Q

D
x
E

R

L

P

L

V
x
M

S

S

L
|
L

K
x
S

A
x
M

R
|
R

Q

E

-

T

-

L

-

N

-

L

-

E

-

P

-

R

A

A

A

A

A

A

S

A

V

V

G

G

Q

Q

S

S

A

G

L

L

I

M

H

S

T

L

Y

Y

E

D

K

A

M

M

L

F

Q

A

E

Q

C

Y

A

G

Q

-

P

-

E

-

N

-

D

-

R

-

L

V

Y

K

C

I

G

A

Q

Q

V

V

L

L

L

V

T

T

H

K

D

P

D

D

L

F

R

Y

T

N

R

E

R

E

R

T

Y

R

L

N

N

N

A

L

R

F

S

C

T

T

L

L

R

S

T

E

L

L

L

I

D

S

M

L

N

N

V

I

L

V

P

P

I

I

I

I

N
|
N

E
x
T

N

N

D
|
D

V

A

V

V

S

S

Y

P

S

P

A
x
M

-

F

I

I

N

P

L

I

G

K

D

D

N
|
N

D

D

T

S

L

L

A

S

A

A

L

M

V

L

S

A

N

A

L

E

L

V

D

Q

A

A

D

D

I

L

L

L

V

I

I

L

L

M

T

S

D

D

Q

V

T

D

G

G

L

I

F

Y

D

N

A

K

D

P

P

P

R

W

S

E

H

D

P

G

E

-

A

A

T

K

L

L

L

M

T

H

E

T

V

Y

G

T

A

S

G

D

D

D

P

-

M

-

L

-

E

-

R

-

I

-

A

-

G

-

G

-

G

-

G

-

S

-

R

-

M

-

G

S

T

N

G

S

G

I

M

M

L

D

A

S

K

K

V

V

R

K

A

A

A

A

A

T

R

W

A

A

A

L

R

D

S

R

G

G

T

V

S

S

T

V

I

V

I

I

A

C

D

N

G

G

R

M

R

Q

P

E

E

K

V

A

L

I

P

K

R

T

L

I

H

I

Q

G

G

G

E

R

A

K

I

V

G

G

T

T

F

F

V

F

binding gamma-glutamyl phosphate: S59 (= S72), A61 (≠ S74), N149 (= N162), T150 (≠ E163), D152 (= D165), M158 (≠ A171), N165 (= N177)
binding : E72 (≠ D86), M75 (≠ V89), L77 (= L91), S78 (≠ K92), M79 (≠ A93), R80 (= R94)

7f5xA Gk domain of drosophila p5cs filament with glutamate (see paper)
30% identity, 67% coverage: 16:283/399 of query aligns to 3:234/236 of 7f5xA

query
sites
7f5xA

W

F

L

T

N

E

R

R

S

S

N

Q

L

L

Q

K

T

Y

D

-

A

A

K

R

R

R

W

L

V

V

I

V

K

K

I

L

G

G

S

S

S

A

L

V

L

I

T

T

N

R

D

E

G

D

Q

N

H

H

-

G

L

L

D

A

I

L

T

G

A

R

I

L

R

A

A

S

W

I

M

V

Q

E

Q

Q

I

V

L

A

L

E

L

C

H

H

Q

L

R

E

G

G

V

R

E

E

V

V

V

M

L

M

V

V

S

T

S
|
S

G

G

S
x
A

V

V

S

A

A

F

G

G

M

K

Q

Q

R

K

L

L

G

A

W

-

T

Q

D

E

R

L

P

L

V

M

S

S

L

L

K

S

A

M

R

R

-

P

Q

R

A

A

A

A

A

A

S

A

V

V

G

G

Q

Q

S

S

A

G

L

L

I

M

H

S

T

L

Y

Y

E

D

K

A

M

M

L

F

Q

A

E

Q

C

Y

A

G

Q

-

P

-

E

-

N

-

D

-

R

-

L

V

Y

K

C

I

G

A

Q

Q

V

V

L

L

L

V

T

T

H

K

D

P

D

D

L

F

R

Y

T

N

R

E

R

E

R

T

Y

R

L

N

N

N

A

L

R

F

S

C

T

T

L

L

R

S

T

E

L

L

L

I

D

S

M

L

N

N

V

I

L

V

P

P

I

I

I

I

N
|
N

E

T

N

N

D
|
D

V

A

V

V

S

S

Y

-

S

-

A

-

I

-

N

-

L

-

G

P

D

D

N

N

D

D

T

S

L

L

A

S

A

A

L

M

V

L

S

A

N

A

L

E

L

V

D

Q

A

A

D

D

I

L

L

L

V

I

I

L

L

M

T

S

D

D

Q

V

T

D

G

G

L

I

F

Y

D

N

A

K

D

P

P

P

R

W

S

E

H

D

P

-

E

-

A

-

T

-

L

-

L

-

T

-

E

-

V

-

G

-

A

-

G

-

D

-

P

-

M

-

L

-

E

-

R

-

I

-

A

-

G

-

G

-

G

G

G

A

S

K

R

L

M

M

G

H

T

T

G

-

G

-

M

Y

L

T

A

S

K

K

V

V

R

K

A

A

A

A

A

T

R

W

A

A

A

L

R

D

S

R

G

G

T

V

S

S

T

V

I

V

I

I

A

C

D

N

G

G

R

M

R

Q

P

E

E

K

V

A

L

I

P

K

R

T

L

I

H

I

Q

G

G

G

E

R

A

K

I

V

G

G

T

T

F

F

V

F

binding gamma-l-glutamic acid: S59 (= S72), A61 (≠ S74), N143 (= N162), D146 (= D165)

8j0gB Gk monomer complexes with glutamate and atp
34% identity, 65% coverage: 25:284/399 of query aligns to 6:268/274 of 8j0gB

query
sites
8j0gB

D

D

A

V

K

K

R

R

W

I

V

V

I

V

K
|
K

I

V

G
|
G

S
x
T

S
x
A

L

V

L

V

T

T

N

G

D

K

G

G

Q

G

H

R

L

L

D

A

I

L

T

G

A

R

I

L

R

G

A

A

W

I

M

C

Q

E

Q

Q

I

L

L

A

L

E

L

L

H

N

Q

S

R

D

G

G

V

F

E

E

V

V

V

I

L

L

V

V

S

S

S

S

G

G

S
x
A

V

V

S

G

A

L

G

G

M

R

Q

Q

R

R

L

L

G

R

W
x
Y

-

R

-

Q

-
x
L

-
x
V

-

N

-

S

-

S

-
x
F

-

A

-

D

T

L

D

Q

R

K

P

P

V

Q

S

M

L

E

K

L

A

D

R

G

Q

K

A

A

A

C

A

A

S

G

V

V

G

G

Q

Q

S

S

A

S

L

L

I

M

H

A

T

Y

Y

Y

E

E

K

T

M

M

L

F

Q

D

E

Q

C

L

A

-

Q

-

P

-

E

-

N

-

D

-

R

D

L

V

Y

T

C

V

G

A

Q

Q

V

M

L

L

L

V

T

T

H

D

D

S

D

S

L

F

R

R

T

D

R

K

R

D

R

F

Y

R

L

K

N

Q

A

L

R

S

S

E

T

T

L

V

R

K

T

A

L

M

L

L

D

R

M

M

N

R

V

V

L

I

P

P

I

V

I

F

N
|
N

E

E

N

N

D
|
D

V

A

V

I

S

S

Y

T

S

R

A

R

I

T

N

G

L

I

G

F

-

W

D

D

N
|
N

D

D

T

S

L

L

A

A

A

A

L

L

V

L

S

S

N

L

L

E

L

L

D

K

A

A

D

D

I

L

L

L

V

I

I

L

L

L

T

S

D

D

Q
x
V

T

E

G

G

L

L

F

Y

D

T

A
x
G

D

P

P
|
P

R

S

S

D

H

S

P

T

E

S

A

K

T

L

L

I

L

H

T

T

E

F

V

I

G

-

A

-

G

K

D

E

P

K

M

H

L

Q

E

D

R

E

I

I

A

T

G
x
F

G

G

G

E

G

K

S

S

R

K

M

L

G

G

T
x
R

G
|
G

G
|
G

M
|
M

L

T

A

A

K
|
K

V

V

R

K

A

A

A

A

A

V

R

N

A

A

A

A

R

Y

S

G

G

G

T

V

S

P

T

V

I

I

I

I

A

T

D

S

G

G

R

Y

R

A

P

A

E

E

V

N

L

I

P

S

R

K

L

V

H

L

Q

R

G

G

E

L

A

R

I

V

G

G

T

T

F

L

V

F

H

H

binding adenosine-5'-triphosphate: K13 (= K32), G15 (= G34), T16 (≠ S35), A17 (≠ S36), V185 (≠ Q199), G191 (≠ A205), P193 (= P207), F215 (≠ G231), R223 (≠ T239), G225 (= G241), M226 (= M242), K229 (= K245)
binding gamma-l-glutamic acid: A55 (≠ S74), N147 (= N162), D150 (= D165), N163 (= N177), R223 (≠ T239)
binding magnesium ion: R223 (≠ T239), G224 (= G240), G225 (= G241)
binding : Y65 (≠ W84), L68 (vs. gap), V69 (vs. gap), F73 (vs. gap)

8j0eB Gk monomer complexes with catalytic intermediate
33% identity, 65% coverage: 25:284/399 of query aligns to 9:263/269 of 8j0eB

query
sites
8j0eB

D

D

A

V

K

K

R

R

W

I

V

V

I

V

K
|
K

I

V

G
|
G

S
x
T

S
x
A

L

V

L

V

T

T

N

G

D

K

G

G

Q

G

H

R

L

L

D

A

I

L

T

G

A

R

I

L

R

G

A

A

W

I

M

C

Q

E

Q

Q

I

L

L

A

L

E

L

L

H

N

Q

S

R

D

G

G

V

F

E

E

V

V

V

I

L

L

V

V

S

S

S
|
S

G
|
G

S

A

V
|
V

S

G

A

L

G

G

M

R

Q

Q

R

R

L

L

G

R

W
x
Y

-

R

-

Q

-
x
L

-
x
V

-

N

-
x
S

-

S

-
x
F

-

A

-

D

T

L

D
x
Q

R

K

P

P

V

Q

S

M

L

E

K

L

A

D

R

G

Q

K

A

A

A

C

A

A

S

G

V

V

G

G

Q

Q

S

S

A

S

L

L

I

M

H

A

T

Y

Y

Y

E

E

K

T

M

M

L

F

Q

D

E

Q

C

L

A

-

Q

-

P

-

E

-

N

-

D

-

R

D

L

V

Y

T

C

V

G

A

Q

Q

V

M

L

L

L

V

T

T

H

D

D

S

D

S

L

F

R

R

T

D

R

K

R

D

R

F

Y

R

L

K

N

Q

A

L

R

S

S

E

T

T

L

V

R

K

T

A

L

M

L

L

D

R

M

M

N

R

V

V

L

I

P

P

I

V

I

F

N

N

E

E

N

N

D
|
D

V

A

V

I

S

S

Y

-

S

-

A

-

I

T

N

R

L

F

G

W

D

D

N
|
N

D
|
D

T

S

L

L

A

A

A

A

L

L

V

L

S

S

N

L

L

E

L

L

D

K

A

A

D

D

I

L

L

L

V

I

I

L

L

L

T

S

D

D

Q
x
V

T

E

G

G

L

L

F

Y

D

T

A

G

D

P

P
|
P

R

S

S

D

H

S

P

T

E

S

A

K

T

L

L

I

L

H

T

T

E

F

V

I

G

-

A

-

G

K

D

E

P

K

M

H

L

Q

E

D

R

E

I

I

A

T

G

F

G

G

G

E

G
x
K

S
x
R

R

-

M

-

G

-

T

-

G
|
G

G
|
G

M
|
M

L

T

A

A

K

K

V

V

R

K

A

A

A

A

A

V

R

N

A

A

A

A

R

Y

S

G

G

G

T

V

S

P

T

V

I

I

I

I

A

T

D

S

G

G

R

Y

R

A

P

A

E

E

V

N

L

I

P

S

R

K

L

V

H

L

Q

R

G

G

E

L

A

R

I

V

G

G

T

T

F

L

V

F

H

H

binding magnesium ion: D163 (= D178), G219 (= G240)
binding (2~{R})-5-[[[[(2~{R},3~{S},4~{S},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-2-azanyl-5-oxidanylidene-pentanoic acid: K16 (= K32), G18 (= G34), T19 (≠ S35), A20 (≠ S36), S56 (= S72), G57 (= G73), V59 (= V75), D153 (= D165), N162 (= N177), V184 (≠ Q199), P192 (= P207), K217 (≠ G234), R218 (≠ S235), G219 (= G240), G220 (= G241), M221 (= M242)
binding : Y68 (≠ W84), L71 (vs. gap), V72 (vs. gap), V72 (vs. gap), S74 (vs. gap), F76 (vs. gap), F76 (vs. gap), Q80 (≠ D86)

8j0fA Gk tetramer with adjacent hooks at reaction state (see paper)
33% identity, 65% coverage: 25:284/399 of query aligns to 10:262/270 of 8j0fA

query
sites
8j0fA

D

D

A

V

K

K

R

R

W

I

V

V

I

V

K
|
K

I

V

G

G

S
x
T

S
x
A

L

V

L

V

T

T

N

G

D

K

G

G

Q

G

H

R

L

L

D

A

I

L

T

G

A

R

I

L

R

G

A

A

W

I

M

C

Q

E

Q

Q

I

L

L

A

L

E

L

L

H

N

Q

S

R

D

G

G

V

F

E

E

V

V

V

I

L

L

V

V

S

S

S
|
S

G

G

S

A

V

V

S

G

A

L

G

G

M

R

Q

Q

R

R

L

L

G

R

W

Y

-

R

-

Q

-
x
L

-
x
V

-
x
N

-
x
S

-
x
S

-
x
F

-
x
A

-

D

T
x
L

D

Q

R

K

P

P

V

Q

S

M

L

E

K

L

A

D

R

G

Q

K

A

A

A

C

A

A

S

G

V

V

G

G

Q

Q

S

S

A

S

L

L

I

M

H

A

T

Y

Y

Y

E

E

K

T

M

M

L

F

Q

D

E

Q

C

L

A

-

Q

-

P

-

E

-

N

-

D

-

R

D

L

V

Y

T

C

V

G

A

Q

Q

V

M

L

L

L

V

T

T

H

D

D

S

D

S

L

F

R

R

T

D

R

K

R

D

R

F

Y

R

L

K

N

Q

A

L

R

S

S

E

T

T

L

V

R

K

T

A

L

M

L

L

D

R

M

M

N

R

V

V

L

I

P

P

I

V

I

F

N

N

E

E

N

N

D
|
D

V

A

V

I

S

S

Y

-

S

-

A

-

I

-

N

T

L

R

G

W

D

D

N
|
N

D

D

T

S

L

L

A

A

A

A

L

L

V

L

S

S

N

L

L

E

L

L

D

K

A

A

D

D

I

L

L

L

V

I

I

L

L

L

T

S

D
|
D

Q
x
V

T

E

G

G

L

L

F
x
Y

D

T

A
x
G

D

P

P

P

R

S

S

D

H

S

P

T

E

S

A

K

T

L

L

I

L

H

T

T

E

F

V

I

G

-

A

-

G

K

D

E

P

K

M

H

L

Q

E

D

R

E

I

I

A

T

G
x
F

G

G

G

-

G

-

S

-

R

-

M

-

G

E

T
x
R

G
|
G

G
|
G

M
|
M

L

T

A

A

K

K

V

V

R

K

A

A

A

A

A

V

R

N

A

A

A

A

R

Y

S

G

G

G

T

V

S

P

T

V

I

I

I

I

A

T

D

S

G

G

R

Y

R

A

P

A

E

E

V

N

L

I

P

S

R

K

L

V

H

L

Q

R

G

G

E

L

A

R

I

V

G

G

T

T

F

L

V

F

H

H

binding adenosine-5'-diphosphate: A21 (≠ S36), D183 (= D198), V184 (≠ Q199), Y188 (≠ F203), G190 (≠ A205), F214 (≠ G231), G219 (= G241), M220 (= M242)
binding magnesium ion: R217 (≠ T239), G218 (= G240), G219 (= G241)
binding gamma-glutamyl phosphate: K17 (= K32), T20 (≠ S35), S57 (= S72), D154 (= D165), N162 (= N177), R217 (≠ T239)
binding : L72 (vs. gap), V73 (vs. gap), N74 (vs. gap), S75 (vs. gap), S76 (vs. gap), F77 (vs. gap), F77 (vs. gap), A78 (vs. gap), L80 (≠ T85)

Q8U122 Uridylate kinase; UK; Uridine monophosphate kinase; UMP kinase; UMPK; EC 2.7.4.22 from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) (see paper)
32% identity, 27% coverage: 177:284/399 of query aligns to 120:224/225 of Q8U122

query
sites
Q8U122

N
x
T

D
|
D

T

A

L

V

A

A

A

A

L

L

V

L

S

A

N

E

L

F

L

L

D

K

A

A

D

D

I

L

L

L

V

V

I

V

L

I

T

T

D

N

Q

V

T

D

G

G

L

V

F

Y

D

T

A

A

D

D

P

P

R

K

S

K

H

D

P

P

E

T

A

A

T

K

L

K

L

I

T

K

E

K

V

M

G

K

A

P

G

-

D

E

P

E

M

L

L

L

E

E

R

-

I

I

A

V

G

G

G

K

G

G

G

I

S

E

R

K

M

A

G
|
G

T

S

G

S

G
x
S

M

V

L

I

A

D

K

P

V

L

R

-

A

A

A

A

A

K

R

I

A

I

A

A

R

R

S

S

G

G

T

I

S

K

T

T

I

I

I

V

A

I

D

G

G

K

R

E

R

D

P

A

E

K

V

D

L

L

P

F

R

R

L

V

H

I

Q

K

G

G

E

D

A

H

I

N

G

G

T

T

F

T

V

I

H

E

T120 (≠ N177) binding Mg(2+)
D121 (= D178) binding Mg(2+); binding Mg(2+)
G179 (= G238) binding UMP
S182 (≠ G241) binding Mg(2+)

Sites not aligning to the query:

6 binding Mg(2+)
44 binding UMP
66 binding UMP
114:120 binding UMP

2bmuB Ump kinase from pyrococcus furiosus complexed with its substrate ump and its substrate analog amppnp (see paper)
32% identity, 27% coverage: 177:284/399 of query aligns to 121:225/226 of 2bmuB

query
sites
2bmuB

N
x
T

D
|
D

T

A

L

V

A

A

A

A

L

L

V

L

S

A

N

E

L

F

L

L

D

K

A

A

D

D

I

L

L

L

V

V

I

V

L

I

T
|
T

D
x
N

Q

V

T

D

G

G

L
x
V

F
x
Y

D

T

A
|
A

D
|
D

P
|
P

R

K

S

K

H

D

P

P

E

T

A

A

T

K

L

K

L

I

T

K

E

K

V

M

G

K

A

P

G

-

D

E

P

E

M

L

L

L

E

E

R

-

I

I

A

V

G

G

G

K

G

G

G

I

S

E

R

K

M
x
A

G

G

T

S

G
x
S

G
x
S

M
x
V

L

I

A

D

K

P

V

L

R

-

A

A

A

A

A

K

R

I

A

I

A

A

R

R

S

S

G

G

T

I

S

K

T

T

I

I

I

V

A

I

D

G

G

K

R

E

R

D

P

A

E

K

V

D

L

L

P

F

R

R

L

V

H

I

Q

K

G

G

E

D

A

H

I

N

G

G

T

T

F

T

V

I

H

E

binding phosphoaminophosphonic acid-adenylate ester: T141 (= T197), N142 (≠ D198), V146 (≠ L202), Y147 (≠ F203), A149 (= A205), D150 (= D206), P151 (= P207), S182 (≠ G240), S183 (≠ G241), V184 (≠ M242)
binding magnesium ion: D122 (= D178), D122 (= D178), S183 (≠ G241), V184 (≠ M242)
binding uridine-5'-monophosphate: T121 (≠ N177), A179 (≠ M237)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 9, 10, 44, 45, 46
binding magnesium ion: 7
binding uridine-5'-monophosphate: 44, 45, 67, 70, 71, 114, 115, 116, 119, 120

Query Sequence

>WP_012536755.1 NCBI__GCF_000021485.1:WP_012536755.1
MTDRIGKKRATEPNRWLNRSNLQTDAKRWVIKIGSSLLTNDGQHLDITAIRAWMQQILLL
HQRGVEVVLVSSGSVSAGMQRLGWTDRPVSLKARQAAASVGQSALIHTYEKMLQECAQPE
NDRLYCGQVLLTHDDLRTRRRYLNARSTLRTLLDMNVLPIINENDVVSYSAINLGDNDTL
AALVSNLLDADILVILTDQTGLFDADPRSHPEATLLTEVGAGDPMLERIAGGGGSRMGTG
GMLAKVRAAARAARSGTSTIIADGRRPEVLPRLHQGEAIGTFVHARLPKLAARKRWLAGH
LRSHGVLHLDQGAARAILEEGSSLLPVGVIAVEGTFRRGDLVVCKDPLGRDIARGLINLD
APVAARCCRKQSHELADDPEVLEDVLIHRDNLVINDLLL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory