SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012537567.1 NCBI__GCF_000021485.1:WP_012537567.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 18 hits to proteins with known functional sites (download)

Q9X0C7 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase; Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase; EC 5.3.1.16 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
34% identity, 95% coverage: 1:231/242 of query aligns to 1:231/241 of Q9X0C7

query
sites
Q9X0C7

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D8 (= D8) mutation to N: Loss of activity.
H48 (= H48) mutation to A: Decrease in activity.
D51 (= D51) mutation to N: Decrease in activity.
R83 (= R85) mutation to N: Decrease in activity.
D127 (= D130) mutation to N: Almost no activity.
T164 (= T167) mutation to A: Strong decrease in activity.

5dn1A Crystal structure of phosphoribosyl isomerase a from streptomyces coelicolor (see paper)
32% identity, 97% coverage: 3:236/242 of query aligns to 6:235/240 of 5dn1A

query
sites
5dn1A

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active site: D11 (= D8), D130 (= D130)
binding aminoimidazole 4-carboxamide ribonucleotide: G23 (= G20), L54 (= L52), F58 (≠ V56), S81 (≠ G81), G83 (= G83), R85 (= R85), G104 (= G104), T105 (= T105), G140 (= G140), W141 (= W141)

P16250 Phosphoribosyl isomerase A; 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase; N-(5'-phosphoribosyl)anthranilate isomerase; PRAI; Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase; EC 5.3.1.16; EC 5.3.1.24 from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) (see paper)
32% identity, 97% coverage: 3:236/242 of query aligns to 6:235/240 of P16250

query
sites
P16250

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D11 (= D8) mutation to A: No activity.
R19 (= R16) mutation to A: No effect on activity toward PRA. No activity toward ProFAR.
S81 (≠ G81) mutation to T: No activity toward PRA. Almost no effect on activity toward ProFAR.
D130 (= D130) mutation to A: Very low activity toward PRA. No activity toward ProFAR.; mutation to Q: No activity.
T166 (= T167) mutation to A: No activity.
D171 (= D172) mutation to A: Low activity toward PRA. No activity toward ProFAR.

3zs4A Crystal structure of mycobacterium tuberculosis phosphoribosyl isomerase with bound prfar
33% identity, 98% coverage: 1:238/242 of query aligns to 3:240/244 of 3zs4A

query
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3zs4A

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active site: D10 (= D8), D129 (= D130)
binding phosphoric acid mono-[5-({[5-carbamoyl-3-(5-phosphonooxy-5-deoxy-ribofuranosyl)- 3h-imidazol-4-ylamino]-methyl}-amino)-2,3,4-trihydroxy-pentyl] ester: A8 (= A6), D10 (= D8), R18 (= R16), A56 (= A55), F57 (≠ V56), S80 (≠ G81), G82 (= G83), R84 (= R85), G103 (= G104), T104 (= T105), D129 (= D130), G143 (= G140), W144 (= W141), D174 (= D172), G175 (= G173), G200 (= G198), G201 (= G199), I223 (= I221), G225 (= G223), K226 (≠ R224)

2y88A Crystal structure of mycobacterium tuberculosis phosphoribosyl isomerase (variant d11n) with bound prfar (see paper)
33% identity, 98% coverage: 1:238/242 of query aligns to 3:240/244 of 2y88A

query
sites
2y88A

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A

S

N

S

L

L

E

D

Q

D

V

L

L

R

A

A

L

I

K

A

A

T

H

L

E

T

S

H

D

R

G

G

I

V

T

E

G

G

A

A

I

I

T

V

G
|
G

R
x
K

A

A

I

L

Y

Y

E

A

G

R

T

R

L

F

D

T

F

L

S

P

Q

Q

A

A

R

L

A

A

active site: N10 (≠ D8), D129 (= D130)
binding [(2r,3s,4r,5r)-5-[4-aminocarbonyl-5-[[(z)-[(3r,4r)-3,4-dihydroxy-2-oxo-5-phosphonooxy-pentyl]iminomethyl]amino]imidazol-1-yl]-3,4-dihydroxy-oxolan-2-yl]methyl dihydrogen phosphate: A8 (= A6), N10 (≠ D8), R18 (= R16), G22 (= G20), L53 (= L52), A56 (= A55), S80 (≠ G81), G81 (= G82), G82 (= G83), R84 (= R85), G103 (= G104), T104 (= T105), D129 (= D130), G143 (= G140), W144 (= W141), D174 (= D172), G175 (= G173), S199 (= S197), G200 (= G198), G225 (= G223), K226 (≠ R224)

4tx9A Crystal structure of hisap from streptomyces sviceus with degraded profar (see paper)
31% identity, 97% coverage: 3:236/242 of query aligns to 11:240/246 of 4tx9A

query
sites
4tx9A

L

L

I

L

P

P

A

A

I

V

D
|
D

L

V

K

R

G

D

G

G

N

Q

C

A

V

V

R

R

L

L

R

V

Q

H

G
|
G

R

E

M

S

E

G

D

T

D

E

T

T

V

S

F

Y

S

G

D

S

D

-

P

P

V

L

A

E

T

A

A

A

Q

L

R

S

W

W

V

Q

E

R

A

A

G

G

A

A

K

E

R

W

L

L

H

H

I

L

V
|
V

D

D

L
|
L

D

D

G

A

A

A

V

F

Q

-

G

G

E

T

P

G

V

D

N

N

A

R

H

E

A

L

I

V

A

R

A

Q

I

V

C

T

A

E

G

A

F

M

P

-

D

D

L

I

E

K

I

V

Q

E

V

L

G
x
S

G

G

G
|
G

I

I

R
|
R

S

D

E

D

E

A

Q

S

I

L

E

A

T

A

Y

A

I

L

Q

A

A

T

G

G

V

C

R

T

Y

R

V

V

I

N

I

L

G
|
G

T
|
T

Q

A

A

A

V

L

K

E

A

S

P

P

G

E

F

W

V

V

A

A

D

K

A

V

T

I

V

A

S

E

F

H

P

G

G

D

H

R

I

I

M

A

V

V

G

G

I

L

D
|
D

A

V

R

R

D

G

G

T

K

T

V

L

A

K

T

G

E

R

G
|
G

W
|
W

S

T

K

S

L

-

S

E

R

G

H

G

D

D

P

L

I

Y

D

E

L

A

A

L

Q

E

R

R

F

L

A

D

A

K

D

E

G

G

I

C

E

A

A

R

I

Y

I

V

Y

V

T

T

D

D

I

I

S

A

R

K

D

D

G

G

M

T

L

L

S

Q

G

G

P

P

N

N

I

L

S

E

A

L

T

L

V

R

A

N

L

V

A

C

Q

A

A

A

V

T

P

D

V

R

P

P

V

V

I

V

A

A

S

S

G

G

I

V

A

S

N

S

L

L

E

D

Q

D

V

L

L

R

A

A

L

I

K

A

A

E

H

L

E

V

S

P

D

L

G

G

I

V

T

E

G

G

A

S

I

I

T

V

G

G

R

K

A

A

I

L

Y

Y

E

A

G

K

T

A

L

F

D

T

F

L

S

E

Q

E

A

A

active site: D16 (= D8), D135 (= D130)
binding aminoimidazole 4-carboxamide ribonucleotide: G28 (= G20), V57 (= V50), L59 (= L52), S86 (≠ G81), G88 (= G83), R90 (= R85), G109 (= G104), T110 (= T105), D135 (= D130), G145 (= G140), W146 (= W141)

5abtA S.Enterica hisa mutant d7n, g102a, v106m, d176a
33% identity, 97% coverage: 2:236/242 of query aligns to 1:238/246 of 5abtA

query
sites
5abtA

L

M

L

I

I

I

P

P

A

A

I

L

D
x
N

L

L

K

I

G

D

G

G

N

T

C

V

V

V

R

R

L

L

R

H

Q

Q

G
|
G

R

D

M

Y

E

A

D

R

D

Q

T

R

V

D

F

Y

S

G

D

N

D

D

P

P

V

L

A

P

T

R

A

L

Q

Q

R

D

W

Y

V

A

E

A

A

Q

G

G

A

A

K

G

R

V

L

L

H

H

I

L

V
|
V

D

D

L
|
L

D

T

G

G

A

A

V

K

Q

D

G

P

E

A

P

K

V

R

N

Q

A

I

H

P

A

L

I

I

A

K

A

T

I

L

C

V

A

A

G

G

F

V

P

-

D

N

L

V

E

P

I

V

Q

Q

V

V

G

G

G
|
G

I

V

R
|
R

S

T

E

E

Q

D

I

V

E

A

T

A

Y

L

I

L

Q

K

A

A

G

G

V

V

R

A

Y

R

V

V

I
x
V

I

I

G
x
A

T
x
S

Q

T

A

A

V

M

K

K

A

S

P

P

G

D

F

V

V

V

A

K

D

G

A

W

T

F

V

E

S

R

F

F

P

G

G

A

H

Q

I

A

M

L

V

V

-

L

G

A

I

L

D
|
D

A

V

R

R

-

I

-

D

-

E

-

H

-

G

D

T

G

K

K

Q

V

V

A

A

T

V

E

S

G
|
G

W
|
W

S

Q

K

E

L

N

S

S

R

G

H

V

D

S

P

L

I

E

D

Q

L

L

A

V

Q

E

R

T

F

Y

A

L

A

P

D

V

G

G

I

L

E

K

A

H

I

V

I

L

Y

C

T

T

D

D

I

I

S

S

R

R

D

A

G
|
G

M

T

L

L

S

A

G

G

P

S

N

N

I

V

S

S

A

L

T

Y

V

E

A

E

L

V

A

C

Q

A

A

R

V

Y

P

P

-

Q

V

I

P

A

V

F

I

Q

A

S

S
|
S

G
|
G

G

G

I

I

A

G

N

D

L

I

E

D

Q

D

V

I

L

A

A

A

L

L

K

R

A

G

H

-

E

-

S

-

D

T

G

G

I

V

T

R

G

G

A

V

I
|
I

T

V

G
|
G

R
|
R

A

A

I

L

Y

L

E

E

G

G

T

K

L

F

D

T

F

V

S

K

Q

E

A

A

active site: N7 (≠ D8), D129 (= D130)
binding [(2R,3S,4R,5R)-5-[4-aminocarbonyl-5-[(E)-[[(2R,3R,4S,5R)-3,4-bis(oxidanyl)-5-(phosphonooxymethyl)oxolan-2-yl]amino]methylideneamino]imidazol-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl dihydrogen phosphate: N7 (≠ D8), G19 (= G20), V49 (= V50), L51 (= L52), G81 (= G83), R83 (= R85), V100 (≠ I102), A102 (≠ G104), S103 (≠ T105), D129 (= D130), G144 (= G140), W145 (= W141), G177 (= G173), S202 (= S197), G203 (= G198), I223 (= I221), G225 (= G223), R226 (= R224)

2y85A Crystal structure of mycobacterium tuberculosis phosphoribosyl isomerase with bound rcdrp (see paper)
32% identity, 98% coverage: 1:238/242 of query aligns to 3:231/234 of 2y85A

query
sites
2y85A

M

L

L

I

L

L

I

L

P

P

A

A

I

V

D
|
D

L

V

K

V

G

E

G

G

N

R

C

A

V

V

R

R

L

T

R

E

Q

Y

G

G

R

S

M

A

E

V

D

D

D

-

T

-

V

-

F

-

S

-

D

-

P

-

V

-

A

-

T

A

A

A

Q

L

R

G

W

W

V

Q

E

R

A

D

G

G

A

A

K

E

R

W

L

I

H
|
H

I

L

V
|
V

D

D

L
|
L

D

D

G

A

A
|
A

V

F

Q

-

G

G

E

R

P

G

V

S

N

N

A

-

H

H

A

E

I

L

A

L

A

A

I

E

C

V

A

V

G

G

F

K

P

L

D

D

L

V

E

Q

I

V

Q

E

V

L

G
x
S

G

G

I

I

R

R

S

D

E

D

E

E

Q

S

I

L

E

A

T

A

Y

A

I

L

Q

A

A

T

G

G

V

C

R

A

Y

R

V

V

I

N

I

V

G

G

T

T

Q

A

A

A

V

L

K

E

A

N

P

P

G

Q

F

W

V

C

A

A

D

R

A

V

T

I

V

G

S

E

F

H

P

G

G

D

H

Q

I

V

M

A

V

V

G

G

I

L

D
|
D

A

V

R

Q

-

I

D

D

G

G

-

E

-

H

K

R

V

L

A

R

T

G

E
x
R

G

G

W

W

S

-

K

E

L

T

S

D

R

G

H

G

D

D

P

L

I

W

D

D

L

V

A

L

Q

E

R

R

F

L

A

D

A

S

D

E

G

G

I

C

E

S

A

R

I

F

I

V

Y

V

T

T

D

D

I

I

S

T

R

K

D
|
D

G
|
G

M

T

L

L

S

G

G

G

P

P

N

N

I

L

S

D

A

L

T

L

V

A

A

G

L

V

A

A

Q

D

A

R

V

T

P

D

V

A

P

P

V

V

I

I

A

A

S
|
S

G
|
G

I

V

A

S

N

S

L

L

E

D

Q

D

V

L

L

R

A

A

L

I

K

A

A

T

H

L

E

T

S

H

D

R

G

G

I

V

T

E

G

G

A

A

I

I

T

V

G
|
G

R
x
K

A

A

I

L

Y

Y

E

A

G

R

T

R

L

F

D

T

F

L

S

P

Q

Q

A

A

R

L

A

A

active site: D10 (= D8), D120 (= D130)
binding 1-(o-carboxy-phenylamino)-1-deoxy-d-ribulose-5-phosphate: D10 (= D8), H40 (= H48), V42 (= V50), L44 (= L52), A47 (= A55), S71 (≠ G81), R133 (≠ E139), D165 (= D172), G166 (= G173), S190 (= S197), G191 (= G198), G192 (= G199), G216 (= G223), K217 (≠ R224)

5ab3A S.Enterica hisa mutant d7n, d10g, dup13-15, q24l, g102a (see paper)
33% identity, 97% coverage: 2:236/242 of query aligns to 1:235/241 of 5ab3A

query
sites
5ab3A

L

M

L

I

I

I

P

P

A

A

I

L

D
x
N

L

L

K

I

G

G

N

T

C

V

V

V

R

R

-

V

-
x
R

L

L

R

H

Q

Q

G

G

-

D

-
x
Y

-

A

R

R

M

L

E

R

D

D

D

-

T

-

V

-

F

Y

S

G

D

N

D

D

P

P

V

L

A

P

T

R

A

L

Q

Q

R

D

W

Y

V

A

E

A

A

Q

G

G

A

A

K

G

R

V

L

L

H

H

I

L

V
|
V

D

D

L

L

D

T

G

G

A

A

V

K

Q

D

G

P

E

A

P

K

V

R

N

Q

A

I

H

P

A

L

I

I

A

K

A

T

I

L

C

V

A

A

G

G

F

V

P

-

D

N

L

V

E

P

I

V

Q

Q

V

V

G

G

G
|
G

I

V

R

R

S

T

E

E

Q

D

I

V

E

A

T

A

Y

L

I

L

Q

K

A

A

G

G

V

V

R

A

Y

R

V

V

I

V

I

I

G
x
A

T
x
S

Q

T

A

A

V

V

K

K

A

S

P

P

G

D

F

V

V

V

A

K

D

G

A

W

T

F

V

E

S

R

F

F

P

G

G

A

H

Q

I

A

M

L

V

V

-

L

G
x
A

I

L

D
|
D

A

V

R

R

-

I

-

D

-

E

-

H

-

G

D

T

G

K

K

Q

V

V

A

A

T

V

E

S

G

G

W

V

S

S

K

L

L

E

S

Q

R

-

H

-

D

-

P

-

I

-

D

-

L

L

A

V

Q

E

R

T

F

Y

A

L

A

P

D

V

G

G

I

L

E

K

A

H

I

V

I

L

Y

C

T

T

D

D

I

I

S

S

R

R

D

D

G
|
G

M

T

L

L

S

A

G

G

P

S

N

N

I

V

S

S

A

L

T

Y

V

E

A

E

L

V

A

C

Q

A

A

R

V

Y

P

P

-

Q

V

I

P

A

V

F

I

Q

A

S

S
|
S

G
|
G

I

I

A

G

N

D

L

I

E

D

Q

D

V

I

L

A

A

A

L

L

K

R

A

G

H

-

E

-

S

-

D

T

G

G

I

V

T

R

G

G

A

V

I

I

T

V

G
|
G

R
|
R

A

A

I

L

Y

L

E

E

G

G

T

K

L

F

D

T

F

V

S

K

Q

E

A

A

active site: N7 (≠ D8), D132 (= D130)
binding [(2r,3s,4r,5r)-5-[4-aminocarbonyl-5-[[(z)-[(3r,4r)-3,4-dihydroxy-2-oxo-5-phosphonooxy-pentyl]iminomethyl]amino]imidazol-1-yl]-3,4-dihydroxy-oxolan-2-yl]methyl dihydrogen phosphate: N7 (≠ D8), R18 (vs. gap), Y24 (vs. gap), V52 (= V50), G84 (= G83), A105 (≠ G104), S106 (≠ T105), A130 (≠ G128), D132 (= D130), G174 (= G173), S199 (= S197), G200 (= G198), G201 (= G199), G222 (= G223), R223 (= R224)

1h5yB Hisf protein from pyrobaculum aerophilum (see paper)
31% identity, 85% coverage: 1:205/242 of query aligns to 5:212/253 of 1h5yB

query
sites
1h5yB

M

L

L

R

L

I

I

I

P

P

A

C

I

L

D
|
D

L

I

K

D

G

G

N

A

C

K

V

V

R

V

L

V

R
x
K

-

G

-

V

-

N

-

F

Q

Q

G

G

R

I

M

R

E

E

D

-

T

-

V

-

F

-

S

V

D

G

D

D

P

P

V

V

A

E

T

M

A

A

Q

V

R

R

W

Y

V

E

E

E

A

E

G

G

A

A

K

D

R

E

L

I

H

A

I

I

V

L

D

D

L

I

D

T

G

A

A

A

V

P

Q

E

G

G

E

R

P

A

V

T

N

F

A

I

H

D

A

S

I

V

A

K

A

R

I

V

C

A

A

E

G

A

F

V

P

S

D

-

L

I

E

P

I

V

Q

L

V

V

G

G

G
|
G

I

V

R

R

S

S

E

L

E

E

Q

D

I

A

E

T

T

T

Y

L

I

F

Q

R

A

A

G

G

V

A

R

D

Y

K

V

V

I

S

I

V

G
x
N

T
|
T

Q

A

A

A

V

V

K

R

A

N

P

P

G

Q

F

L

V

V

A

A

D

L

A

L

T

A

V

R

S

E

F

F

P

G

G

S

H

Q

-

S

I

T

M

V

V

V

G
x
A

I

I

D
|
D

A

A

R

K

D

W

G

N

K

G

V

E

A

Y

T

Y

E

E

G

V

W

Y

S

V

K

K

L
x
G

S

G

R

R

H

E

-

A

-

T

-

G

-

L

D

D

P

A

I

V

D

K

L

W

A

A

Q

K

R

E

F

V

A

E

D

L

G

G

I

A

E

G

A

E

I

I

I

L

Y

L

T
|
T

D

S

I

I

S

D

R

R

D
|
D

G
|
G

M

T

L

G

S

L

G

G

P

Y

N

D

I

V

S

E

A

L

T

I

V

R

A

R

L

V

A

A

Q

D

A

S

V

V

P

R

V

I

P

P

V

V

I

I

A

A

S

S

G

G

I

A

A

G

N

R

L

V

E

E

Q

H

active site: D12 (= D8), D133 (= D130)
binding glycerol: K22 (≠ R18), N106 (≠ G104), A131 (≠ G128), D133 (= D130), G147 (≠ L144), T174 (= T167), D179 (= D172)
binding phosphate ion: G84 (= G82), G85 (= G83), N106 (≠ G104), T107 (= T105), D179 (= D172), G180 (= G173)

Sites not aligning to the query:

binding phosphate ion: 226, 227, 228

P60664 Imidazole glycerol phosphate synthase subunit HisF; IGP synthase cyclase subunit; IGP synthase subunit HisF; ImGP synthase subunit HisF; IGPS subunit HisF; EC 4.3.2.10 from Escherichia coli (strain K12) (see paper)
24% identity, 98% coverage: 3:238/242 of query aligns to 6:246/258 of P60664

query
sites
P60664

L

I

I

I

P

P

A

C

I

L

D

D

L

V

K

R

G

D

G

G

N

Q

C

V

V

V

R

K

L

G

R

V

Q

Q

G

F

R

R

M

N

E

H

D

E

D

-

T

-

V

-

F

I

S

I

D

G

D

D

P

I

V

V

A

P

T

L

A

A

Q

K

R

R

W

Y

V

A

E

E

A

E

G

G

A

A

K

D

R
x
E

L

L

H

V

I

F

V

Y

D

D

L

I

D

T

G

A

A

S

V

S

Q

D

G

G

E

R

P

V

V

V

N

D

A

K

H

S

A

W

I

V

A

S

A

R

I

V

C

-

A

A

G

E

F

V

P

I

D

D

L

I

E

P

I

F

Q

C

V

V

G

A

G

G

I

I

R

K

S

S

E

L

E

E

Q

D

I

A

E

A

T

K

Y

I

I

L

Q

S

A

F

G

G

V

A

R

D

Y

K

V

I

I

S

I

I

G

N

T

S

Q

P

A

A

V

L

K

A

A

D

P

P

G

T

F

L

V

I

A

T

D

R

A

L

T

A

V

D

S

R

F

F

P

G

G

V

H
x
Q

-
x
C

I

I

M

V

V

V

G

G

I

I

D

D

-

T

-

W

-

Y

-

D

A

A

R

E

D

T

G

G

K

K

-

Y

-

H

-

V

-

N

-

Q

-

Y

V

T

A

G

T

D

E

E

G

S

W

R

S

T

K

R

L

V

S

T

R

Q

H

W

D

E

P

T

I

L

D

D

L

W

A

V

Q

Q

R

E

F

V

A

Q

A

K

D

R

G

G

I

A

E

G

A

E

I

I

I

V

Y

L

T

N

D

M

I

M

S

N

R

Q

D

D

G

G

M

V

L

R

S

N

G

G

P

Y

N

D

I

L

S

E

A

Q

T

L

V

K

A

K

L

V

A

R

Q

E

A

V

V

C

P

H

V

V

P

P

V

L

I

I

A

A

S

S

G

G

I

A

A

G

N

T

L

M

E

E

Q

H

V

F

L

-

A

-

L

L

K

E

A

A

H

F

E

R

S

D

D

A

G

D

I

V

T

D

G

G

A

A

I

L

T

A

G

A

R

S

A

V

I

F

Y

H

E

K

G

Q

T

I

L

I

D

N

F

I

S

G

Q

E

A

L

R

K

A

A

E46 (≠ R46) mutation to A: Loss of activity.; mutation to G: Loss of IGP synthase activity. Weak IGP synthase and HisH activities in vitro.
Q123 (≠ H124) mutation to A: Decrease in activity.; mutation to R: Loss of IGP synthase activity. Weak HisH activity in vitro.
C124 (vs. gap) mutation to A: No change in activity.; mutation to R: Loss of IGP synthase activity. Weak HisH activity in vitro.

Sites not aligning to the query:

5 R→A: Loss of activity.; R→H: Loss of IGP synthase activity. Weak IGP synthase activity and reduced HisH activity in vitro.

1gpwC Structural evidence for ammonia tunneling across the (beta/alpha)8 barrel of the imidazole glycerol phosphate synthase bienzyme complex. (see paper)
27% identity, 80% coverage: 14:207/242 of query aligns to 9:211/253 of 1gpwC

query
sites
1gpwC

C

C

V

L

R
x
N

L

V

R

K

Q

D

G

G

R

R

M

V

E

V

D

K

D

G

T

T

V

N

F

F

-

E

-

N

-

L

-

R

-

D

S

S

D

G

D

D

P

P

V

V

A

E

T

L

A

G

Q

K

R

F

W

Y

V

S

E

E

A

I

G

G

A

I

K

D

R

E

L

L

H

V

I

F

V

L

D

D

L

I

D

T

G

A

A

S

V

V

Q

E

G

K

E

R

P

K

V

T

N

M

A

L

H

E

A

L

I

V

A

E

A

K

I

V

C

A

A

E

G

Q

F

I

P

-

D

D

L

I

E

P

I

F

Q

T

V

V

G

G

G
|
G

I

I

R

H

S

D

E

F

E

E

Q

T

I

A

E

S

T

E

Y

L

I

I

Q

L

A

R

G

G

V

A

R

D

Y

K

V

V

I

S

I

I

G
x
N

T
|
T

Q

A

A

A

V

V

K

E

A

N

P

P

G

S

F

L

V

I

A

T

D

Q

A

I

T

A

V

Q

S

T

F

F

P

G

G

S

H

Q

-

A

I

V

M

V

V

V

G

A

I

I

D
|
D

A

A

R

K

-

R

-

V

D

D

G

G

K

E

V

F

A

M

T

V

E

F

G

T

W

Y

S

S

K

G

-

K

-

N

-

T

-

G

-

I

L

L

S

L

R

R

H

D

D

W

P

V

I

V

D

E

L

V

A

E

Q

K

R

R

F

G

A

A

A

G

D

E

G

-

I

-

E

-

A

-

I

I

I

L

Y

L

T

T

D

S

I

I

S

D

R

R

D
|
D

G
|
G

M

T

L

K

S

S

G

G

P

Y

N

D

I

T

S

E

A

M

T

I

V

R

A

F

L

V

A

R

Q

P

A

L

V

T

P

T

V

L

P

P

V

I

I

I

A

A

S

S

G

G

I

A

A

G

N

K

L

M

E

E

Q

H

V

F

L

L

active site: N11 (≠ R16), D130 (= D130)
binding phosphate ion: G82 (= G83), N103 (≠ G104), T104 (= T105), D176 (= D172), G177 (= G173)

Sites not aligning to the query:

binding phosphate ion: 225

Q9X0C6 Imidazole glycerol phosphate synthase subunit HisF; IGP synthase cyclase subunit; IGP synthase subunit HisF; ImGP synthase subunit HisF; IGPS subunit HisF; EC 4.3.2.10 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
27% identity, 80% coverage: 14:207/242 of query aligns to 9:211/253 of Q9X0C6

query
sites
Q9X0C6

C
|
C

V

L

R
x
D

L

V

R

K

Q

D

G

G

R

R

M

V

E

V

D
x
K

D

G

T

T

V

N

F

F

-

E

-

N

-

L

-

R

-

D

S

S

D

G

D

D

P

P

V

V

A

E

T

L

A

G

Q

K

R

F

W

Y

V

S

E

E

A

I

G

G

A

I

K

D

R

E

L

L

H

V

I

F

V

L

D
|
D

L

I

D

T

G

A

A

S

V

V

Q

E

G

K

E

R

P

K

V

T

N

M

A

L

H

E

A

L

I

V

A

E

A

K

I

V

C

A

A

E

G

Q

F

I

P

-

D

D

L

I

E

P

I

F

Q

T

V

V

G

G

I

I

R

H

S

D

E

F

E

E

Q

T

I

A

E

S

T

E

Y

L

I

I

Q

L

A

R

G

G

V

A

R

D

Y

K

V

V

I

S

I

I

G
x
N

T

T

Q

A

A

A

V

V

K

E

A

N

P

P

G

S

F

L

V

I

A

T

D

Q

A

I

T

A

V

Q

S

T

F

F

P

G

G

S

H

Q

-

A

I

V

M

V

V

V

G

A

I

I

D
|
D

A

A

R

K

-

R

-

V

D

D

G

G

K

E

V

F

A

M

T

V

E

F

G

T

W

Y

S

S

K

G

-

K

-

N

-

T

-

G

-

I

L

L

S

L

R

R

H

D

D

W

P

V

I

V

D

E

L

V

A

E

Q

K

R

R

F

G

A

A

A

G

D

E

G

-

I

-

E

-

A

-

I

I

I

L

Y

L

T

T

D

S

I

I

S

D

R

R

D
|
D

G

G

M

T

L

K

S

S

G

G

P

Y

N
x
D

I

T

S

E

A

M

T

I

V

R

A

F

L

V

A

R

Q

P

A

L

V

T

P

T

V

L

P

P

V

I

I

I

A

A

S

S

G

G

I

A

A

G

N

K

L

M

E

E

Q

H

V

F

L

L

C9 (= C14) mutation to A: No change in activity.
D11 (≠ R16) mutation to X: Loss of activity.
K19 (≠ D24) mutation to S: Decrease in activity.
D51 (= D51) mutation to N: No change in activity.
N103 (≠ G104) mutation to A: No change in activity.
D130 (= D130) mutation D->A,C,F,G,H,I,K,L,M,N,P,Q,R,S,T,V,W,Y: Complete loss of activity.; mutation to E: Weak activity.
D176 (= D172) mutation to N: Decrease in activity.
D183 (≠ N179) mutation to N: No change in activity.

7ac8A Molecular basis for the unique allosteric activation mechanism of the heterodimeric imidazole glycerol phosphate synthase complex. (see paper)
27% identity, 80% coverage: 14:207/242 of query aligns to 9:211/252 of 7ac8A

query
sites
7ac8A

C

C

V

L

R
x
D

L

V

R

K

Q

D

G

G

R

R

M

V

E

V

D

K

D

G

T

T

V

N

F

F

-

E

-

N

-

L

-

R

-

D

S

S

D

G

D

D

P

P

V

V

A

E

T

L

A

G

Q

K

R

F

W

Y

V

S

E

E

A

I

G

G

A

I

K

D

R

E

L

L

H

V

I

F

V
x
L

D

D

L
x
I

D

T

G

A

A

S

V

V

Q

E

G

K

E

R

P

K

V

T

N

M

A

L

H

E

A

L

I

V

A

E

A

K

I

V

C

A

A

E

G

Q

F

I

P

-

D

D

L

I

E

P

I

F

Q

T

V

V

G

G

G
|
G

I

I

R

H

S

D

E

F

E

E

Q

T

I

A

E

S

T

E

Y

L

I

I

Q

L

A

R

G

G

V

A

R

D

Y

K

V

V

I

S

I

I

G
x
N

T
|
T

Q

A

A

A

V

V

K

E

A

N

P

P

G

S

F

L

V

I

A

T

D

Q

A

I

T

A

V

Q

S

T

F

F

P

G

G

S

H

Q

-

A

I

V

M

V

V

V

G

A

I

I

D
|
D

A

A

R

K

-

R

-

V

D

D

G

G

K

E

V

F

A

M

T

V

E

F

G

T

W

Y

S
|
S

K

G

-

K

-

N

-

T

-

G

-

I

L

L

S

L

R

R

H

D

D

W

P

V

I

V

D

E

L

V

A

E

Q

K

R

R

F

G

A

A

A

G

D

E

G

-

I

-

E

-

A

-

I

I

I

L

Y

L

T

T

D

S

I

I

S

D

R

R

D
|
D

G
|
G

M

T

L

K

S

S

G

G

P

Y

N

D

I

T

S

E

A

M

T

I

V

R

A

F

L

V

A

R

Q

P

A

L

V

T

P

T

V

L

P

P

V

I

I

I

A

A

S
|
S

G

G

I

A

A

G

N

K

L

M

E

E

Q

H

V

F

L

L

active site: D11 (≠ R16), D130 (= D130)
binding [(2R,3S,4R,5R)-5-[4-aminocarbonyl-5-[(E)-[[(2R,3R,4S,5R)-3,4-bis(oxidanyl)-5-(phosphonooxymethyl)oxolan-2-yl]amino]methylideneamino]imidazol-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl dihydrogen phosphate: L50 (≠ V50), I52 (≠ L52), G82 (= G83), N103 (≠ G104), T104 (= T105), D130 (= D130), S144 (= S142), D176 (= D172), G177 (= G173), S201 (= S197)

Sites not aligning to the query:

binding [(2R,3S,4R,5R)-5-[4-aminocarbonyl-5-[(E)-[[(2R,3R,4S,5R)-3,4-bis(oxidanyl)-5-(phosphonooxymethyl)oxolan-2-yl]amino]methylideneamino]imidazol-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl dihydrogen phosphate: 225

3zr4E Structural evidence for ammonia tunneling across the (beta-alpha)8 barrel of the imidazole glycerol phosphate synthase bienzyme complex (see paper)
28% identity, 80% coverage: 14:207/242 of query aligns to 9:202/244 of 3zr4E

query
sites
3zr4E

C

C

V

L

R
x
D

L

V

R

K

Q

D

G

G

R

R

M

V

E

-

D

-

T

-

V

V

F

K

S

S

D

G

D

D

P

P

V

V

A

E

T

L

A

G

Q

K

R

F

W

Y

V

S

E

E

A

I

G

G

A

I

K

D

R

E

L

L

H

V

I

F

V

L

D

D

L

I

D

T

G

A

A

S

V

V

Q

E

G

K

E

R

P

K

V

T

N

M

A

L

H

E

A

L

I

V

A

E

A

K

I

V

C

A

A

E

G

Q

F

I

P

-

D

D

L

I

E

P

I

F

Q

T

V

V

G

G

I

I

R

H

S

D

E

F

E

E

Q

T

I

A

E

S

T

E

Y

L

I

I

Q

L

A

R

G

G

V

A

R

D

Y

K

V

V

I

S

I

I

G

N

T

T

Q

A

A

A

V

V

K

E

A

N

P

P

G

S

F

L

V

I

A

T

D

Q

A

I

T

A

V

Q

S

T

F

F

P

G

G

S

H

Q

-

A

I

V

M

V

V

V

G

A

I

I

D
|
D

A

A

R

K

-

R

-

V

D

D

G

G

K

E

V

F

A

M

T

V

E

F

G

T

W

Y

S

S

K

G

-

K

-

N

-

T

-

G

-

I

L

L

S

L

R

R

H

D

D

W

P

V

I

V

D

E

L

V

A

E

Q

K

R

R

F

G

A

A

A

G

D

E

G

-

I

-

E

-

A

-

I

I

I

L

Y

L

T

T

D

S

I

I

S

D

R

R

D

D

G

G

M

T

L

K

S

S

G

G

P

Y

N

D

I

T

S

E

A

M

T

I

V
x
R

A

F

L

V

A

R

Q

P

A

L

V

T

P

T

V

L

P

P

V

I

I

I

A

A

S

S

G

G

I

A

A

G

N

K

L

M

E

E

Q

H

V

F

L

L

active site: D11 (≠ R16), D121 (= D130)
binding glycerol: R179 (≠ V184)

Sites not aligning to the query:

binding glycerol: 206

7qc8A Imidazole glycerol phosphate synthase subunit HisF (see paper)
27% identity, 85% coverage: 3:207/242 of query aligns to 6:211/250 of 7qc8A

query
sites
7qc8A

L

I

I

I

P

A

A

A

I

L

D
x
E

L

V

K

K

G

D

G

G

N

R

C

V

V

V

R

K

L

-

R

-

Q

-

G

G

R

T

M

N

E

F

D

E

D

N

T

L

V

R

F

D

S

S

D

G

D

D

P

P

V

A

A

E

T

L

A

G

Q

K

R

F

W

Y

V

S

E

E

A

I

G

G

A

I

K

D

R

E

L

L

H

V

I

F

V
x
H

D

D

L
x
H

D

T

G

A

A

S

V

V

Q

E

G

K

E

R

P

K

V

T

N

M

A

L

H

E

A

L

I

V

A

E

A

K

I

V

C

A

A

E

G

Q

F

I

P

-

D

D

L

I

E

P

I

F

Q

T

V

V

G

G

I

I

R

H

S

D

E

F

E

E

Q

T

I

A

E

S

T

E

Y

L

I

I

Q

L

A

R

G

G

V

A

R

D

Y

K

V

V

I

S

I

I

G

N

T

T

Q

A

A

A

V

V

K

E

A

N

P

P

G

S

F

L

V

I

A

T

D

Q

A

I

T

A

V

Q

S

T

F

F

P

G

G

S

H

Q

-

A

I

V

M

V

V

V

G

A

I

I

D

D

A

A

R

K

-

R

-

V

D

D

G

G

K

E

V

F

A

M

T

V

E

F

G

T

W

Y

S

S

K

G

-

K

-

N

-

T

-

G

-

I

L

L

S

L

R

R

H

D

D

W

P

V

I

V

D

E

L

V

A

E

Q

K

R

R

F

G

A

A

A

G

D

E

G

-

I

-

E

-

A

-

I

I

I

L

Y

L

T

T

D

S

I

I

S

D

R

R

D

D

G

G

M

T

L

K

S

S

G

G

P

Y

N

D

I

T

S

E

A

M

T

I

V

R

A

F

L

V

A

R

Q

P

A

L

V

T

P

T

V

L

P

P

V

I

I

I

A

A

S

S

G

G

I

A

A

G

N

K

L

M

E

E

Q

H

V

F

L

L

binding zinc ion: E11 (≠ D8), H50 (≠ V50), H52 (≠ L52)

2wjzE Crystal structure of (hish) k181a y138a mutant of imidazoleglycerolphosphate synthase (hish hisf) which displays constitutive glutaminase activity (see paper)
27% identity, 80% coverage: 14:207/242 of query aligns to 9:197/237 of 2wjzE

query
sites
2wjzE

C

C

V

L

R
x
D

L

V

R

K

Q

D

G

G

R

R

M

V

E

-

D

-

T

-

V

-

F

-

S

V

D

G

D

D

P

P

V

V

A

E

T

L

A

G

Q

K

R

F

W

Y

V

S

E

E

A

I

G

G

A

I

K

D

R

E

L

L

H

V

I

F

V

L

D

D

L

I

D

T

G

A

A

K

V

R

Q

K

G

-

E

-

P

-

V

-

N

T

A

M

H

L

A

E

I

L

A

V

A

E

I

K

C

V

A

A

G

E

F

Q

P

I

D

D

L

I

E

P

I

F

Q

T

V

V

G

G

G
|
G

I

I

R

H

S

D

E

F

E

E

Q

T

I

A

E

S

T

E

Y

L

I

I

Q

L

A

R

G

G

V

A

R

D

Y

K

V

V

I

S

I

I

G
x
N

T
|
T

Q

A

A

A

V

V

K

E

A

N

P

P

G

S

F

L

V

I

A

T

D

Q

A

I

T

A

V

Q

S

T

F

F

P

G

G

S

H

Q

-

A

I

V

M

V

V

V

G

A

I

I

D
|
D

A

A

R

K

-

R

-

V

D

D

G

G

K

E

V

F

A

M

T

V

E

F

G

T

W

Y

S

S

K

G

-

K

-

N

-

T

-

G

-

I

L

L

S

L

R

R

H

D

D

W

P

V

I

V

D

E

L

V

A

E

Q

K

R

R

F

G

A

A

A

G

D

E

G

-

I

-

E

-

A

-

I

I

I

L

Y

L

T

T

D

S

I

I

S

D

R

R

D

D

G

G

M

T

L

K

S

S

G

G

P

Y

N

D

I

T

S

E

A

M

T

I

V

R

A

F

L

V

A

R

Q

P

A

L

V

T

P

T

V

L

P

P

V

I

I

I

A

A

S

S

G

G

I

A

A

G

N

K

L

M

E

E

Q

H

V

F

L

L

active site: D11 (≠ R16), D116 (= D130)
binding phosphate ion: G68 (= G83), N89 (≠ G104), T90 (= T105)

4ewnD Structure of hisf-d130v+d176v with bound rcdrp (see paper)
26% identity, 80% coverage: 14:207/242 of query aligns to 8:204/243 of 4ewnD

query
sites
4ewnD

C

C

V

L

R

D

L

V

R

K

Q

D

G

G

R

R

M

V

E

V

D

K

D

G

T

T

V

N

F

F

-

E

-

N

-

L

-

R

-

D

S

S

D

G

D

D

P

P

V

V

A

E

T

L

A

G

Q

K

R

F

W

Y

V

S

E

E

A

I

G

G

A

I

K

D

R

E

L

L

H

V

I

F

V

L

D

D

L

I

D

T

G

K

A

T

V

M

Q

L

G

-

E

-

P

-

V

-

N

-

A

-

H

-

A

E

I

L

A

V

A

E

I

K

C

V

A

A

G

E

F

Q

P

I

D

D

L

I

E

P

I

F

Q

T

V

V

G

G

I

I

R

H

S

D

E

F

E

E

Q

T

I

A

E

S

T

E

Y

L

I

I

Q

L

A

R

G

G

V

A

R

D

Y

K

V

V

I

S

I

I

G

N

T

T

Q

A

A

A

V

V

K

E

A

N

P

P

G

S

F

L

V

I

A

T

D

Q

A

I

T

A

V

Q

S

T

F

F

P

G

G

S

H

Q

-

A

I

V

M

V

V

V

G

A

I

I

D
x
V

A

A

R

K

-

R

-

V

D

D

G

G

K

E

V

F

A

M

T

V

E

F

G

T

W

Y

S

S

K

G

-

K

-

N

-

T

-

G

-

I

L

L

S

L

R

R

H

D

D

W

P

V

I

V

D

E

L

V

A

E

Q

K

R

R

F

G

A

A

A

G

D

E

G

-

I

-

E

-

A

-

I

I

I

L

Y

L

T

T

D

S

I

I

S

D

R

R

D

V

G
|
G

M

T

L

K

S

S

G

G

P

Y

N

D

I

T

S

E

A

M

T

I

V

R

A

F

L

V

A

R

Q

P

A

L

V

T

P

T

V

L

P

P

V

I

I

I

A

A

S
|
S

G
|
G

I

A

A

G

N

K

L

M

E

E

Q

H

V

F

L

L

binding 1-(O-carboxy-phenylamino)-1-deoxy-D-ribulose-5-phosphate: V123 (≠ D130), G170 (= G173), S194 (= S197), G195 (= G198), G196 (= G199)

Sites not aligning to the query:

binding 1-(O-carboxy-phenylamino)-1-deoxy-D-ribulose-5-phosphate: 215, 216, 217, 218

Query Sequence

>WP_012537567.1 NCBI__GCF_000021485.1:WP_012537567.1
MLLIPAIDLKGGNCVRLRQGRMEDDTVFSDDPVATAQRWVEAGAKRLHIVDLDGAVQGEP
VNAHAIAAICAGFPDLEIQVGGGIRSEEQIETYIQAGVRYVIIGTQAVKAPGFVADATVS
FPGHIMVGIDARDGKVATEGWSKLSRHDPIDLAQRFAADGIEAIIYTDISRDGMLSGPNI
SATVALAQAVPVPVIASGGIANLEQVLALKAHESDGITGAITGRAIYEGTLDFSQARAQA
EA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory