SitesBLAST
Comparing WP_012537708.1 NCBI__GCF_000021485.1:WP_012537708.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1w4zA Structure of actinorhodin polyketide (actiii) reductase (see paper)
36% identity, 81% coverage: 6:216/261 of query aligns to 8:223/259 of 1w4zA
- active site: G15 (= G13), N112 (= N111), S142 (= S137), Y155 (= Y150), K159 (= K154), Y200 (= Y193)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G9), T13 (≠ G11), S14 (= S12), G15 (= G13), I16 (= I14), R36 (= R34), G37 (≠ R35), D61 (= D59), V62 (= V60), N88 (= N86), G90 (= G88), S142 (= S137), Y155 (= Y150), K159 (= K154), P185 (= P180), G186 (= G181), V188 (= V183), T190 (= T185)
P16544 Putative ketoacyl reductase; EC 1.3.1.- from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) (see 2 papers)
36% identity, 81% coverage: 6:216/261 of query aligns to 10:225/261 of P16544
- 11:39 (vs. 7:35, 38% identical) binding NADP(+)
- D63 (= D59) binding NADP(+)
- K161 (= K154) binding NADP(+)
2rh4B Actinorhodin ketoreductase, actkr, with NADPH and inhibitor emodin (see paper)
36% identity, 81% coverage: 6:216/261 of query aligns to 17:232/268 of 2rh4B
- active site: G24 (= G13), N121 (= N111), S151 (= S137), Y164 (= Y150), K168 (= K154), Y209 (= Y193)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G20 (= G9), T22 (≠ G11), S23 (= S12), I25 (= I14), A44 (≠ G33), R45 (= R34), G46 (≠ R35), C69 (≠ V58), D70 (= D59), V71 (= V60), N97 (= N86), S151 (= S137), Y164 (= Y150), K168 (= K154), G195 (= G181), V197 (= V183), T199 (= T185), M201 (= M187)
2rh4A Actinorhodin ketoreductase, actkr, with NADPH and inhibitor emodin (see paper)
36% identity, 81% coverage: 6:216/261 of query aligns to 6:221/257 of 2rh4A
- active site: G13 (= G13), N110 (= N111), S140 (= S137), Y153 (= Y150), K157 (= K154), Y198 (= Y193)
- binding 3-methyl-1,6,8-trihydroxyanthraquinone: T141 (≠ V138), Q145 (≠ R142), V147 (≠ L144), Y153 (= Y150), F185 (≠ Y182)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G9 (= G9), T11 (≠ G11), S12 (= S12), G13 (= G13), I14 (= I14), A33 (≠ G33), R34 (= R34), G35 (≠ R35), C58 (≠ V58), D59 (= D59), V60 (= V60), N86 (= N86), G88 (= G88), S140 (= S137), Y153 (= Y150), K157 (= K154), P183 (= P180), G184 (= G181), V186 (= V183), T188 (= T185), M190 (= M187)
Sites not aligning to the query:
1xr3A Actinorhodin polyketide ketoreductase with NADP and the inhibitor isoniazid bound (see paper)
36% identity, 81% coverage: 6:216/261 of query aligns to 5:220/256 of 1xr3A
- active site: G12 (= G13), N109 (= N111), S139 (= S137), Y152 (= Y150), K156 (= K154), Y197 (= Y193)
- binding 4-(diazenylcarbonyl)pyridine: T140 (≠ V138), G141 (≠ A139), V146 (≠ L144)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G9), T10 (≠ G11), S11 (= S12), G12 (= G13), I13 (= I14), A32 (≠ G33), R33 (= R34), G34 (≠ R35), C57 (≠ V58), D58 (= D59), V59 (= V60), N85 (= N86), A86 (= A87), G87 (= G88), S139 (= S137), Y152 (= Y150), K156 (= K154), G183 (= G181), V185 (= V183), T187 (= T185), P188 (= P186)
4nbuB Crystal structure of fabg from bacillus sp (see paper)
35% identity, 71% coverage: 6:190/261 of query aligns to 11:192/244 of 4nbuB
- active site: G18 (= G13), N111 (= N111), S139 (= S137), Q149 (≠ A147), Y152 (= Y150), K156 (= K154)
- binding acetoacetyl-coenzyme a: D93 (≠ G92), K98 (≠ E97), S139 (= S137), N146 (≠ L144), V147 (≠ P145), Q149 (≠ A147), Y152 (= Y150), F184 (≠ Y182), M189 (= M187)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G9), N17 (≠ S12), G18 (= G13), I19 (= I14), D38 (≠ G33), F39 (≠ R34), V59 (= V58), D60 (= D59), V61 (= V60), N87 (= N86), A88 (= A87), G89 (= G88), I90 (= I89), T137 (≠ I135), S139 (= S137), Y152 (= Y150), K156 (= K154), P182 (= P180), F184 (≠ Y182), T185 (≠ V183), T187 (= T185), M189 (= M187)
Sites not aligning to the query:
Q8KES3 Sepiapterin reductase; SPR; cSR; EC 1.1.1.325 from Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) (Chlorobium tepidum) (see 2 papers)
31% identity, 79% coverage: 1:205/261 of query aligns to 1:215/244 of Q8KES3
- 9:15 (vs. 9:15, 86% identical) binding NADP(+)
- SRT 40:42 (≠ GRR 33:35) binding NADP(+)
- DI 66:67 (≠ DV 59:60) binding NADP(+)
- N93 (= N86) binding NADP(+)
- F99 (vs. gap) binding substrate; mutation to A: Drastically reduces activity. Complete loss of activity; when associated with A-196.
- T116 (= T110) binding NADP(+)
- S145 (= S137) binding substrate
- Y158 (= Y150) binding NADP(+); binding substrate
- K162 (= K154) binding NADP(+)
- VYTPMW 191:196 (≠ VATPM- 183:187) binding NADP(+)
- W196 (vs. gap) binding substrate; mutation to A: Drastically reduces activity. Complete loss of activity; when associated with A-99.
P73574 3-oxoacyl-[acyl-carrier-protein] reductase; 3-ketoacyl-acyl carrier protein reductase; EC 1.1.1.100 from Synechocystis sp. (strain PCC 6803 / Kazusa) (see paper)
35% identity, 75% coverage: 6:201/261 of query aligns to 10:208/247 of P73574
- A14 (= A10) mutation to G: 4.2-fold increase in activity on acetoacetyl-CoA.
- P151 (= P145) mutation to F: 2.7-fold increase in activity on acetoacetyl-CoA.; mutation to V: 5.7-fold increase in activity on acetoacetyl-CoA.
- K160 (= K154) mutation to A: Almost no activity on acetoacetyl-CoA.
- F188 (≠ Y182) mutation to Y: 3.3-fold increase in activity on acetoacetyl-CoA.
- N198 (= N191) mutation to R: 3.5-fold increase in activity on acetoacetyl-CoA.
2bd0D Chlorobium tepidum sepiapterin reductase complexed with NADP and sepiapterin (see paper)
31% identity, 77% coverage: 5:205/261 of query aligns to 4:214/240 of 2bd0D
- active site: S144 (= S137), Y157 (= Y150), K161 (= K154)
- binding biopterin: F98 (vs. gap), S144 (= S137), Y157 (= Y150), M194 (= M187), W195 (vs. gap)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G9), K11 (≠ S12), I13 (= I14), S39 (≠ G33), R40 (= R34), T41 (≠ R35), D65 (= D59), I66 (≠ V60), N92 (= N86), G94 (= G88), V95 (≠ I89), T115 (= T110), S144 (= S137), Y157 (= Y150), K161 (= K154), P187 (= P180), A189 (≠ Y182), V190 (= V183), T192 (= T185), P193 (= P186), M194 (= M187), W195 (vs. gap)
2bd0A Chlorobium tepidum sepiapterin reductase complexed with NADP and sepiapterin (see paper)
31% identity, 77% coverage: 5:205/261 of query aligns to 4:214/240 of 2bd0A
- active site: S144 (= S137), Y157 (= Y150), K161 (= K154)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G9), K11 (≠ S12), G12 (= G13), I13 (= I14), R40 (= R34), T41 (≠ R35), D65 (= D59), I66 (≠ V60), N92 (= N86), A93 (= A87), G94 (= G88), V95 (≠ I89), T115 (= T110), I142 (= I135), S144 (= S137), K161 (= K154), P187 (= P180), A189 (≠ Y182), V190 (= V183), T192 (= T185), P193 (= P186), M194 (= M187), W195 (vs. gap)
3sj7A Structure of beta-ketoacetyl-coa reductase (fabg) from staphylococcus aureus complex with NADPH (see paper)
32% identity, 75% coverage: 6:201/261 of query aligns to 5:202/239 of 3sj7A
- active site: G12 (= G13), S138 (= S137), Q148 (≠ A147), Y151 (= Y150), K155 (= K154)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G9), S10 (≠ G11), R11 (≠ S12), I13 (= I14), N31 (≠ L31), Y32 (≠ L32), A33 (≠ G33), G34 (≠ R34), S35 (≠ R35), A58 (≠ V58), N59 (≠ D59), V60 (= V60), N86 (= N86), A87 (= A87), T109 (= T110), S138 (= S137), Y151 (= Y150), K155 (= K154), P181 (= P180), G182 (= G181)
1edoA The x-ray structure of beta-keto acyl carrier protein reductase from brassica napus complexed with NADP+ (see paper)
34% identity, 71% coverage: 5:190/261 of query aligns to 4:191/244 of 1edoA
- active site: G12 (= G13), S138 (= S137), Y151 (= Y150), K155 (= K154)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G9), S10 (≠ G11), R11 (≠ S12), I13 (= I14), N31 (≠ L31), Y32 (≠ L32), A33 (≠ G33), R34 (= R34), S35 (≠ R35), D59 (= D59), V60 (= V60), N86 (= N86), A87 (= A87), S138 (= S137), Y151 (= Y150), K155 (= K154), P181 (= P180), G182 (= G181), I184 (≠ V183), S186 (≠ T185), M188 (= M187)
3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
32% identity, 72% coverage: 1:188/261 of query aligns to 3:192/246 of 3osuA
6zzsD Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from acinetobacter baumannii complexed with NAD+ and 3-oxovalerate (see paper)
34% identity, 69% coverage: 7:187/261 of query aligns to 12:193/261 of 6zzsD
- active site: G18 (= G13), S143 (= S137), Y156 (= Y150)
- binding nicotinamide-adenine-dinucleotide: G14 (= G9), S17 (= S12), I19 (= I14), D38 (≠ G33), M39 (≠ R34), D64 (= D59), V65 (= V60), N91 (= N86), A92 (= A87), G93 (= G88), M141 (≠ I135), A142 (= A136), S143 (= S137), Y156 (= Y150), K160 (= K154), P186 (= P180), G187 (= G181), V189 (= V183), T191 (= T185), L193 (≠ M187)
- binding 3-oxidanylidenepentanoic acid: Q95 (≠ S90), S143 (= S137), N145 (≠ A139), K153 (≠ A147), Y156 (= Y150)
Sites not aligning to the query:
6zzqA Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from acinetobacter baumannii complexed with NAD+ and acetoacetate (see paper)
34% identity, 69% coverage: 7:187/261 of query aligns to 11:192/260 of 6zzqA
- active site: G17 (= G13), S142 (= S137), Y155 (= Y150)
- binding acetoacetic acid: Q94 (≠ S90), S142 (= S137), K152 (≠ A147), Y155 (= Y150)
- binding nicotinamide-adenine-dinucleotide: G13 (= G9), S16 (= S12), G17 (= G13), I18 (= I14), D37 (≠ G33), M38 (≠ R34), D63 (= D59), V64 (= V60), N90 (= N86), A91 (= A87), G92 (= G88), M140 (≠ I135), A141 (= A136), S142 (= S137), Y155 (= Y150), K159 (= K154), Y187 (= Y182), V188 (= V183), T190 (= T185)
Sites not aligning to the query:
7emgB Carbonyl reductase variant 4 (r123c/l209p/f183y/v61k) from serratia marcescens complexed with NADP+ (see paper)
35% identity, 70% coverage: 5:188/261 of query aligns to 7:188/243 of 7emgB
4bmsF Short chain alcohol dehydrogenase from ralstonia sp. Dsm 6428 in complex with NADPH
36% identity, 72% coverage: 6:192/261 of query aligns to 10:191/249 of 4bmsF
- active site: S137 (= S137), H147 (≠ A147), Y150 (= Y150), K154 (= K154)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G9), N15 (≠ G11), S16 (= S12), I18 (= I14), R38 (= R34), R39 (= R35), A59 (≠ V58), D60 (= D59), V61 (= V60), N87 (= N86), S88 (≠ A87), G89 (= G88), V110 (≠ T110), S137 (= S137), Y150 (= Y150), K154 (= K154), G181 (= G181), I183 (≠ V183), T185 (= T185), I187 (≠ M187)
Sites not aligning to the query:
2cfcA Structural basis for stereo selectivity in the (r)- and (s)- hydroxypropylethane thiosulfonate dehydrogenases (see paper)
34% identity, 70% coverage: 6:188/261 of query aligns to 6:193/250 of 2cfcA
- active site: G13 (= G13), S142 (= S137), Y155 (= Y150), K159 (= K154)
- binding (2-[2-ketopropylthio]ethanesulfonate: F149 (≠ L144), R152 (≠ A147), Y155 (= Y150)
- binding nicotinamide-adenine-dinucleotide: G9 (= G9), S12 (= S12), G13 (= G13), N14 (≠ I14), D33 (≠ G33), L34 (≠ R34), A59 (≠ V58), D60 (= D59), V61 (= V60), N87 (= N86), A88 (= A87), G89 (= G88), I140 (= I135), P185 (= P180), G186 (= G181), M187 (≠ Y182), I188 (≠ V183), T190 (= T185), P191 (= P186), M192 (= M187), T193 (= T188)
Sites not aligning to the query:
Q56840 2-(R)-hydroxypropyl-CoM dehydrogenase; R-HPCDH; 2-[(R)-2-hydroxypropylthio]ethanesulfonate dehydrogenase; Aliphatic epoxide carboxylation component III; Epoxide carboxylase component III; RHPCDH1; EC 1.1.1.268 from Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2) (see 4 papers)
34% identity, 70% coverage: 6:188/261 of query aligns to 6:193/250 of Q56840
- SGN 12:14 (≠ SGI 12:14) binding NAD(+)
- D33 (≠ G33) binding NAD(+)
- DV 60:61 (= DV 59:60) binding NAD(+)
- N87 (= N86) binding NAD(+)
- S142 (= S137) mutation to A: Retains weak activity. 120-fold decrease in kcat.; mutation to C: Loss of activity.
- R152 (≠ A147) binding 2-oxopropyl-coenzyme M; mutation to A: Almost loss of activity with the natural substrate 2-KPC, but does not affect activity with 2-butanone as substrate.
- Y155 (= Y150) mutation Y->E,F: Loss of activity.
- K159 (= K154) mutation to A: Loss of activity.
- R179 (= R174) mutation to A: Loss of activity.
- IETPM 188:192 (≠ VATPM 183:187) binding NAD(+)
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 195:196 binding 2-oxopropyl-coenzyme M
- 196 R→A: Almost loss of activity with the natural substrate 2-KPC, but does not affect activity with 2-butanone as substrate.
- 203 R→A: Slight decrease in catalytic efficiency.
- 209 R→A: Does not affect catalytic efficiency.
6ihhA Crystal structure of rasadh f12 from ralstonia.Sp in complex with NADPH and a6o
35% identity, 71% coverage: 6:191/261 of query aligns to 10:191/249 of 6ihhA
- binding (2R,3S)-2-ethyl-2-[(2E)-2-(6-methoxy-3,4-dihydro-2H-naphthalen-1-ylidene)ethyl]-3-oxidanyl-cyclopentan-1-one: S137 (= S137), H147 (≠ A147), Y150 (= Y150), L188 (≠ T188)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G9), N15 (≠ G11), S16 (= S12), G17 (= G13), I18 (= I14), R38 (= R34), R39 (= R35), D60 (= D59), V61 (= V60), N87 (= N86), S88 (≠ A87), G89 (= G88), V110 (≠ T110), T135 (≠ I135), S137 (= S137), Y150 (= Y150), K154 (= K154), P180 (= P180), G181 (= G181), A182 (≠ Y182), I183 (≠ V183), T185 (= T185), S187 (≠ M187)
Sites not aligning to the query:
Query Sequence
>WP_012537708.1 NCBI__GCF_000021485.1:WP_012537708.1
MMGAVVITGAGSGIGRALALAYAEPGRVVLLLGRRRTALQETAVQVRARGARAELRTVDV
CDGAALDRIAADFAGGFGSVAVLIANAGISHGTLTAEPADRTVFAEIVATNLIGVELTCG
AFLPYLAPQARIAGIASVAGFRGLPGAAAYSASKAGAIAYLESLRLELRDRGIRVCSIAP
GYVATPMTAENHYPMPWLMPVDQAAAKMRRAIDRGRPWCVLPWQMAVLGTLLRHLPIAIY
DPIFARVAGKRRRTGDDAAES
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory