SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012540670.1 NCBI__GCF_000025465.1:WP_012540670.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

Q9XDN5 Phosphate propanoyltransferase; Phosphate acyltransferase PduL; Phosphotransacylase PduL; PTAC; Propanediol utilization protein PduL; EC 2.3.1.222 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
83% identity, 99% coverage: 1:207/210 of query aligns to 1:207/210 of Q9XDN5

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DKEL 2:5 (≠ DKQQ 2:5) mutation Missing: Decreased amount of PduL in BMCs, enzyme has wild-type activity.
2:10 (vs. 2:10, 67% identical) mutation Missing: Considerably decreased amount of PduL in BMCs, enzyme has greater than wild-type activity.

Q21A54 Phosphate propanoyltransferase; Phosphate acyltransferase PduL; Phosphotransacylase PduL; PTAC; Propanediol utilization protein PduL; EC 2.3.1.222 from Rhodopseudomonas palustris (strain BisB18) (see paper)
52% identity, 82% coverage: 22:194/210 of query aligns to 40:212/226 of Q21A54

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VSN 44:46 (≠ ISS 26:28) binding CoA
H48 (= H30) binding Zn(2+)
H50 (= H32) binding Zn(2+)
M72 (≠ L53) binding CoA
K90 (= K71) binding CoA
R97 (= R78) binding CoA
R103 (= R84) binding phosphate
E109 (= E90) binding Zn(2+)
F116 (≠ R97) binding CoA
H157 (= H138) binding Zn(2+)
H159 (= H140) binding Zn(2+)
H204 (= H186) binding Zn(2+)
N211 (= N193) binding CoA

Sites not aligning to the query:

1:33 mutation Missing: Enzyme active, produces crystals.

5cupA Structure of rhodopseudomonas palustris pdul - phosphate bound form (see paper)
52% identity, 82% coverage: 22:194/210 of query aligns to 5:177/189 of 5cupA

query
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binding phosphate ion: S17 (≠ A34), T19 (≠ E36), D20 (= D37), R68 (= R84), S92 (= S108), H124 (= H140)
binding zinc ion: H13 (= H30), H15 (= H32), E74 (= E90), H122 (= H138), H124 (= H140), H169 (= H186)

5cuoB Structure of rhodopseudomonas palustris pdul - coa bound form (see paper)
52% identity, 82% coverage: 22:194/210 of query aligns to 5:177/189 of 5cuoB

query
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5cuoB

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binding coenzyme a: V9 (≠ I26), S10 (= S27), N11 (≠ S28), H13 (= H30), H15 (= H32), M37 (≠ L53), K55 (= K71), R62 (= R78), L64 (= L80), A78 (≠ T94), F81 (≠ R97), V172 (≠ T189), N176 (= N193)
binding zinc ion: H13 (= H30), H15 (= H32), E74 (= E90), H122 (= H138), H124 (= H140), H169 (= H186)

Query Sequence

>WP_012540670.1 NCBI__GCF_000025465.1:WP_012540670.1
MDKQQLASTVNKVLDEMRQRPIPIGISSRHIHLAAEDYARLFPAQPIQPKKALLQPGQYA
AEQTVTLVGPKGRLNNVRLLGPLRQTSQVEISRTDARTLGIAAPLRMSGNLQDTPGIRLI
SPFAELELSSGTIVAQRHIHMSPLDALILRVAHGDSVAVAIEGSDRRLIFDNVAVRVAPD
MRLEMHIDTDEANAAGADAAQAWATLVTKP

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory