SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012540845.1 NCBI__GCF_000025465.1:WP_012540845.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 10 hits to proteins with known functional sites (download)

P0A9J8 Bifunctional chorismate mutase/prephenate dehydratase; Chorismate mutase-prephenate dehydratase; P-protein; EC 5.4.99.5; EC 4.2.1.51 from Escherichia coli (strain K12)
89% identity, 100% coverage: 1:385/386 of query aligns to 1:385/386 of P0A9J8

query
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P0A9J8

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R11 (= R11) mutation R->A,K: Important decrease in catalytic efficiency and affinity.
R28 (= R28) mutation R->A,K: Important decrease in catalytic efficiency and affinity.
K39 (= K39) mutation K->A,Q,R: Important decrease in catalytic efficiency and affinity.
E52 (= E52) mutation E->A,D,Q: Important decrease in catalytic efficiency and affinity.
Q88 (= Q88) mutation Q->A,E,K: Important decrease in catalytic efficiency and affinity.

1ecmB Atomic structure of the buried catalytic pocket of escherichia coli chorismate mutase
92% identity, 25% coverage: 6:100/386 of query aligns to 1:95/95 of 1ecmB

query
sites
1ecmB

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binding 8-hydroxy-2-oxa-bicyclo[3.3.1]non-6-ene-3,5-dicarboxylic acid: R23 (= R28), V30 (= V35), K34 (= K39), R42 (= R47), D43 (= D48), R46 (= R51), E47 (= E52), I76 (= I81), S79 (= S84), Q83 (= Q88)

2qmxA The crystal structure of l-phe inhibited prephenate dehydratase from chlorobium tepidum tls (see paper)
31% identity, 70% coverage: 106:375/386 of query aligns to 4:273/278 of 2qmxA

query
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2qmxA

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active site: T169 (= T278), F171 (= F280)
binding phenylalanine: N204 (≠ Q306), E205 (≠ Q307), L209 (= L311), D222 (≠ I324), L223 (≠ M325), T224 (= T326), K225 (= K327), S228 (= S330), Y238 (≠ E340), F240 (= F342)

6vh5D Crystal structure of prephenate dehydratase from brucella melitensis biovar abortus 2308 in complex with phenylalanine
32% identity, 72% coverage: 105:380/386 of query aligns to 8:281/282 of 6vh5D

query
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6vh5D

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binding phenylalanine: N207 (≠ Q306), V208 (≠ Q307), L212 (= L311), N225 (≠ I324), M226 (= M325), T227 (= T326), K228 (= K327), S231 (= S330), T241 (≠ E340), F243 (= F342)

7am0B Gqqa- a novel type of quorum quenching acylases (see paper)
31% identity, 70% coverage: 106:377/386 of query aligns to 4:271/278 of 7am0B

query
sites
7am0B

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V

G
x
N

Q
x
N

Q
|
Q

A
x
P

G

G

A

A

L
|
L

V

Y

E

K

A

A

L

L

L

G

V

G

L

L

R

A

N

T

H

A

N

G

L

V

I
x
N

M
|
M

T
|
T

K
x
R

L

L

E

E

S

S

R

Y

P

M

I

L

H

E

G

G

N

S

P

F

W

S

E

A

E
x
T

M

Q

F
|
F

Y

L

L

M

D

D

I

V

Q

E

A

G

N

H

L

P

D

E

S

A

L

P

P

P

M

L

R

A

K

R

A

A

L

L

K

D

E

E

L

L

A

S

E

F

I

F

T

S

R

E

S

Q

M

Q

K

E

V

I

L

L

G

G

C

V

Y

Y

P

P

S

A

binding phenylalanine: N199 (≠ G305), N200 (≠ Q306), Q201 (= Q307), P202 (≠ A308), L205 (= L311), N218 (≠ I324), M219 (= M325), T220 (= T326), R221 (≠ K327), T234 (≠ E340), F236 (= F342)

3mwbB The crystal structure of prephenate dehydratase in complex with l-phe from arthrobacter aurescens to 2.0a
25% identity, 69% coverage: 108:375/386 of query aligns to 5:271/303 of 3mwbB

query
sites
3mwbB

F

F

L

L

G

G

P

P

K

Q

G

G

S

T

Y

F

S

T

H

E

L

A

A

A

A

L

R

M

Q

Q

Y

V

A

P

A

G

R

A

H

A

F

D

E

A

Q

T

F

R

I

I

E

P

S

-

G

-

C

C

A

T

K

N

F

V

A

N

D

T

I

A

F

L

N

E

Q

R

V

V

E

R

T

A

G

G

Q

E

A

A

D

D

Y

A

A

A

V

M

V

V

P

P

I

I

E

E

N

N

T

S

S

V

S

E

G

G

I

V

N

T

D

A

V

T

Y

L

D

D

-

A

L

I

L

A

Q

T

H

G

T

Q

S

E

L

L

S

R

I

I

V

I

G

R

E

E

L

A

T

L

I

V

P

P

I

I

D

T

H

F

C

V

V

L

L

V

V

A

S

R

T

P

S

G

T

V

D

E

A

L

D

S

K

D

I

I

Q

K

T

R

V

I

Y

S

S

T

H

H

P

G

Q

H

P

A

F

W

Q

A

Q

Q

C

C

S

R

Q

L

Y

W

L

V

S

D

R

E

Y

H

-

L

P

P

H

N

W

A

K

D

I

Y

E

V

Y

P

T

G

E

S

S

S

T

T

S

A

A

A

A

S

M

A

E

M

K

G

V

L

A

L

Q

E

A

D

N

D

S

A

P

P

A

Y

V

E

A

A

L

I

G

C

S

A

E

P

A

L

G

I

G
x
A

A

A

L

E

Y
x
Q

-

P

S

G

L
|
L

Q

N

V

V

L

L

E

A

H

E

C

D

Q

I

A

G

N

D

Q

N

T

P

Q

D

N

A

I

V

T
|
T

R

R

F
|
F

L

I

V

L

L

V

A

S

R

R

K

P

A

G

V

A

N

L

V

P

S

E

D

R

Q

T

V

G

P

A

A

D

K

K

T

T

L

V

M

V

A

P

T

L

G

P

Q

E

-
x
D

Q
x
H

A

P

G

G

A

A

L
|
L

V

M

E

E

A

I

L

L

L

D

V

Q

L

F

R

A

N

S

H

R

N

G

L

V

I
x
N

M
x
L

T
x
S

K
x
R

L
x
I

E

E

S

S

R

R

P

P

I

T

H

L

G

G

N

H

P

-

W

-

E

-

E
x
Y

M

F

F
|
F

Y

S

L

I

D

D

I

A

Q

D

A

G

N

H

L

A

D

T

S

D

L

S

P

R

M

V

R

A

K

D

A

A

L

L

K

A

E

G

L

L

A

H

E

R

I

I

T

S

R

P

S

A

M

T

K

R

V

F

L

L

G

G

C

S

Y

Y

active site: T176 (= T278), F178 (= F280)
binding magnesium ion: A155 (≠ G258), Q158 (≠ Y261), L161 (= L263)
binding phenylalanine: D205 (vs. gap), H206 (≠ Q307), L210 (= L311), N223 (≠ I324), L224 (≠ M325), S225 (≠ T326), R226 (≠ K327), I227 (≠ L328), Y236 (≠ E340), F238 (= F342)

3mwbA The crystal structure of prephenate dehydratase in complex with l-phe from arthrobacter aurescens to 2.0a
25% identity, 69% coverage: 108:375/386 of query aligns to 5:274/306 of 3mwbA

query
sites
3mwbA

F

F

L

L

G

G

P

P

K

Q

G

G

S

T

Y

F

S

T

H

E

L

A

A

A

A

L

R

M

Q

Q

Y

V

A

P

A

G

R

A

H

A

F

D

E

A

Q

T

F

R

I

I

E

P

S

-

G

-

C

C

A

T

K

N

F

V

A

N

D

T

I

A

F

L

N

E

Q

R

V

V

E

R

T

A

G

G

Q

E

A

A

D

D

Y

A

A

A

V

M

V

V

P

P

I

I

E

E

N

N

T

S

S

V

S

E

G

G

I

V

N

T

D

A

V

T

Y

L

D

D

-

A

L

I

L

A

Q

T

H

G

T

Q

S

E

L

L

S

R

I

I

V

I

G

R

E

E

L

A

T

L

I

V

P

P

I

I

D

T

H

F

C

V

V

L

L

V

V

A

S

R

T

P

S

G

T

V

D

E

A

L

D

S

K

D

I

I

Q

K

T

R

V

I

Y

S

S

T

H

H

P

G

Q

H

P

A

F

W

Q

A

Q

Q

C

C

S

R

Q

L

Y

W

L

V

S

D

R

E

Y

H

-

L

P

P

H

N

W

A

K

D

I

Y

E

V

Y

P

T

G

E

S

S

S

T

T

S

A

A

A

A

S

M

A

E

M

K

G

V

L

A

L

Q

E

A

D

N

D

S

A

P

P

A

Y

V

E

A

A

L

I

G

C

S

A

E

P

A

L

G

I

G

A

A

A

L

E

Y

Q

-

P

S

G

L

L

Q

N

V

V

L

L

E

A

H

E

C

D

Q

I

A

G

N

D

Q

N

T

P

Q

D

N

A

I

V

T
|
T

R

R

F
|
F

L

I

V

L

L

V

A

S

R

R

K

P

A

G

V

A

N

L

V

P

S

E

D

R

Q

T

V

G

P

A

A

D

K

K

T

T

L

V

M

V

A

P

T

L

G

P

Q

E

-
x
D

Q
x
H

A

P

G

G

A

A

L
|
L

V

M

E

E

A

I

L

L

L

D

V

Q

L

F

R

A

N

S

H

R

N

G

L

V

I
x
N

M
x
L

T
x
S

K
x
R

L

I

E

E

S
|
S

R

R

P

P

I

T

H

G

G

Q

N

Y

P

L

W

G

E

H

E

Y

M

F

F
|
F

Y

S

L

I

D

D

I

A

Q

D

A

G

N

H

L

A

D

T

S

D

L

S

P

R

M

V

R

A

K

D

A

A

L

L

K

A

E

G

L

L

A

H

E

R

I

I

T

S

R

P

S

A

M

T

K

R

V

F

L

L

G

G

C

S

Y

Y

active site: T176 (= T278), F178 (= F280)
binding phenylalanine: D205 (vs. gap), H206 (≠ Q307), L210 (= L311), N223 (≠ I324), L224 (≠ M325), S225 (≠ T326), R226 (≠ K327), S229 (= S330), F241 (= F342)

7alzA Gqqa- a novel type of quorum quenching acylases (see paper)
32% identity, 37% coverage: 237:377/386 of query aligns to 52:187/194 of 7alzA

query
sites
7alzA

A

A

M

C

E

E

K

N

V

S

A

L

Q

A

A

G

N

R

S

V

P

P

A

D

V

I

A

H

A

A

L

L

G

L

S

P

E

E

A

A

G

G

G

-

A

-

L

-

Y

-

S

L

L

F

Q

I

V

V

L

G

E

E

H

H

C

F

Q

Q

A

-

N

-

Q

R

T

V

Q

H

N

N

I

T

T

T

R

R

F

F

L

Y

V

I

L

A

A

S

R

R

K

R

A

P

V

A

N

T

V

L

S

P

D

P

Q

P

V

G

P

P

A

G

-

F

K

M

T

T

L

L

M

F

A

R

T

V

G

N

Q

N

Q
|
Q

A

P

G

G

A

A

L
|
L

V

Y

E

K

A

A

L

L

L

G

V

G

L

L

R

A

N

T

H

A

N

G

L

V

I
x
N

M
|
M

T
|
T

K
x
R

L

L

E

E

S

S

R

Y

P

M

I

L

H

E

G

G

N

S

P

F

W

S

E

A

E
x
T

M

Q

F
|
F

Y

L

L

M

D

D

I

V

Q

E

A

G

N

H

L

P

D

E

S

A

L

P

P

P

M

L

R

A

K

R

A

A

L

L

K

D

E

E

L

L

A

S

E

F

I

F

T

S

R

E

S

Q

M

Q

K

E

V

I

L

L

G

G

C

V

Y

Y

P

P

S

A

binding phenylalanine: Q117 (= Q307), L121 (= L311), N134 (≠ I324), M135 (= M325), T136 (= T326), R137 (≠ K327), T150 (≠ E340), F152 (= F342)

3luyA Putative chorismate mutase from bifidobacterium adolescentis
26% identity, 71% coverage: 103:376/386 of query aligns to 2:284/326 of 3luyA

query
sites
3luyA

S

S

A

A

R

R

V

K

A

L

F

F

-

Y

L

L

G

G

P

P

K

Q

G

G

S

T

Y

F

S

T

H

H

L

Q

A

A

R

V

Q

N

Y

-

A

A

R

Q

H

E

F

L

E

A

Q

R

F

F

I

E

E

P

S

Q

G

G

-

F

-

D

-

L

-

M

-

P

C

M

A

D

K

D

F

V

A

P

D

Q

I

I

F

L

N

D

Q

A

V

A

E

Q

T

H

G

G

Q

D

A

G

D

-

Y

W

A

G

V

I

V

V

P

A

I

W

E

E

N

N

T

N

S

V

S

E

G

G

G

Y

I

V

N

V

D

P

V

N

Y

L

D

D

-

A

L

L

L

I

Q

D

H

A

T

K

S

D

L

L

S

V

I

G

V

F

G

A

E

R

L

V

T

G

I

V

P

N

I

V

D

E

H

F

C

D

V

A

L

Y

V

V

S

A

T

Q

S

G

T

A

D

D

A

P

D

A

K

E

I

A

Q

R

T

I

V

A

Y

T

S

A

H

H

P

P

Q

H

P

G

F

L

Q

A

Q

Q

C

C

S

K

Q

R

Y

F

L

I

S

A

R

-

Y

-

P

-

H

-

W

-

K

-

I

-

E

E

Y

H

T

R

E

L

S

S

T

T

S

Q

A

P

A

A

M

T

E

S

K

N

V

A

A

A

Q

A

A

C

N

R

S

D

-

L

-

I

P

P

A

G

V

E

A

I

A

A

L

F

G

G

S

P

E

A

A

I

G

C

G

G

A

E

L

L

Y

Y

S

D

L

I

Q

T

V

R

L

I

E

G

H

T

C

A

Q

I

A

Q

N

D

Q

Y

T

Q

Q

G

N

A

I

A

T
|
T

R

D

F

F

L

L

V

V

L

L

A

S

R

P

K

R

A

A

-

E

-

V

-

A

-

R

-

L

-

A

-

K

-

P

-

R

-

A

-

E

V

A

N

N

V

V

S

E

D

Y

Q

E

V

S

P

-

A

V

K

L

T

T

T

L

L

I

L

P

M

L

A

V

T

T

G
|
G

Q

-

A

P

G

G

A

V

L
|
L

V

A

E

N

A

L

L

L

L

D

V

V

L

F

R

R

N

D

H

A

N

G

L

L

I

N

M

M

T

T

K

S

L

F

E

I

S

S

R

R

P

P

I

I

H

K

G

G

N

R

-

T

-

G

-

T

-
x
Y

-

S

-
x
F

-

I

-

V

-

T

-

L

-

D

-

A

P

P

W

W

E

E

M

R

F

F

Y

-

L

-

D

-

I

-

Q

-

A

-

N

-

L

-

D

-

S

-

L

-

P

-

M

-

R

R

K

D

A

A

L

L

K

V

E

E

L

I

A

A

E

E

I

H

T

G

R

D

S

W

M

A

K

K

V

T

L

L

G

A

C

V

Y

Y

P

P

active site: T177 (= T278)
binding 3-phenylpyruvic acid: G215 (= G305), L219 (= L311), Y248 (vs. gap), F250 (vs. gap)

2gtvX Nmr structure of monomeric chorismate mutase from methanococcus jannaschii (see paper)
32% identity, 23% coverage: 7:96/386 of query aligns to 5:102/104 of 2gtvX

query
sites
2gtvX

L

L

L

A

A

E

L

I

R
|
R

D

K

K

K

I

I

S

D

A

E

L

I

D

D

E

N

K

K

L

I

L

L

A

K

-

A

-

R

-

W

-

P

-

W

-

A

-

E

-

K

L

L

L

I

A

A

E

E

R
|
R

R

N

G

S

L

L

A

A

V

K

E

D

V
|
V

G

A

K

E

A

I

K
|
K

L

N

A

Q

S

L

H

G

R

I

P

P

V

I

R

N

D
|
D

I

P

D

E

R
|
R

E
|
E

R

K

D

Y

L

I

L

Y

E

D

R

R

L

I

M

R

T

K

I

L

G

C

K

K

R

E

H

H

N

N

L

V

D

D

A

E

H

N

Y

I

I

G

T

I

R

K

L

I

F

F

Q

Q

L

R

I

L

I

I

E

E

D

H

S
x
N

V

K

L

A

T

L

Q
|
Q

Q

K

T

Q

L

Y

L

L

Q

E

H

T

L

L

binding 8-hydroxy-2-oxa-bicyclo[3.3.1]non-6-ene-3,5-dicarboxylic acid: R9 (= R11), R34 (= R28), V41 (= V35), K45 (= K39), D54 (= D48), R57 (= R51), E58 (= E52), N90 (≠ S84), Q94 (= Q88)

Query Sequence

>WP_012540845.1 NCBI__GCF_000025465.1:WP_012540845.1
MTEENPLLALRDKISALDEKLLALLAERRGLAVEVGKAKLASHRPVRDIDRERDLLERLM
TIGKRHNLDAHYITRLFQLIIEDSVLTQQTLLQQHLNKINPHSARVAFLGPKGSYSHLAA
RQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIENTSSGGINDVYDLLQHTSLSI
VGELTIPIDHCVLVSTSTDADKIQTVYSHPQPFQQCSQYLSRYPHWKIEYTESTSAAMEK
VAQANSPAVAALGSEAGGALYSLQVLEHCQANQTQNITRFLVLARKAVNVSDQVPAKTTL
LMATGQQAGALVEALLVLRNHNLIMTKLESRPIHGNPWEEMFYLDIQANLDSLPMRKALK
ELAEITRSMKVLGCYPSENVVPVDPV

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SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory