SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012542131.1 NCBI__GCF_000025465.1:WP_012542131.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

2gvnA L-asparaginase from erwinia carotovora in complex with aspartic acid (see paper)
75% identity, 93% coverage: 24:347/347 of query aligns to 2:325/325 of 2gvnA

query
sites
2gvnA

L

L

P

P

H

N

I

I

V

V

I

I

L

L

A

A

T

T

G

G

G

G

T
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T

I

I

A

A

G

G

S

S

A

A

A

A

S

A

N

N

T

T

Q

Q

T

T

T

T

G

G

Y
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Y

K

K

A

A

G

G

A

A

I

L

G

G

V

V

Q

E

T

T

L

L

I

I

N

Q

A

A

V

V

P

P

E

E

M

L

S

K

K

T

I

L

A

A

H

N

V

I

E

K

G

G

E

E

Q

Q

V

V

A

A

N

S

I

I

G
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G

S
|
S

E
|
E

N

N

M

M

T

T

S

S

D

D

I

V

I

L

L

L

E

T

L

L

S

S

K

K

R

R

V

V

N

N

A

E

L

L

L

L

A

A

R

R

D

S

D

D

V

V

D

D

G

G

V

V

V

V

I

I

T

T

H

H

G
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G

T
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T

D
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D

T

T

L

L

D

D

E

E

T

S

P

P

Y

Y

F

F

L

L

N

N

L

L

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T

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V

K

K

S

S

N

D

K

K

P

P

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V

F

F

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A

A

A

A

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M

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R

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P

A

A

T

T

A

A

I

I

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S

A

A

D

D

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G

P

P

M

M

N

N

L

L

L

Y

E

G

A

A

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T

K

V

V

A

A

A

A

D

D

P

K

D

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A

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M

L

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L

L

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N

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D

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R

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I

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G

A

S

A

A

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R

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F

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K
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K

T

T

N

N

A

A

T

S

S

T

L

L

D

D

T

T

F

F

R

K

A

A

P

P

E

E

E

E

G

G

Y

Y

L

L

G

G

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V

I

V

I

G

G

G

D

K

K

P

I

Q

Y

F

Y

E

Q

T

T

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R

V

L

D

D

K

K

I

V

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H

T

T

L

T

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R

S

S

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V

F

F

D

D

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V

R

T

Q

N

L

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K

D

T

K

L

L

P

P

K

A

V

V

V

D

I

I

I

I

Y

Y

G

G

Y

Y

Q

Q

D

D

D

D

P

P

E

E

Y

Y

M

M

Y

Y

D

D

A

A

A

S

I

I

A

K

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H

G

A

V

D

K

G

G

I

I

I

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Y

Y

A

A

G

G

T

M

G

G

A

A

G

G

S

S

V

V

S

S

V

K

R

R

S

G

A

D

A

A

G

G

I

I

K

R

K

K

A

A

Q

E

Q

S

A

K

G

G

I

I

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V

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R

A

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S

S

R

R

T

T

G

G

S

S

G

G

V

I

V

V

P

P

P

P

D

D

D

A

S

G

Q

Q

P

P

G

G

L

L

V

V

S

A

D

D

S

S

L

L

N

S

P

P

A

A

K

K

A

S

R

R

I

I

L

L

L

L

M

M

T

L

A

A

L

L

T

T

Q

K

T

T

K

T

D

N

P

P

Q

A

L

V

I

I

Q

Q

Q

D

Y

Y

F

F

H

H

T

A

Y

Y

active site: T13 (= T35), Y27 (= Y49), T93 (= T115), D94 (= D116), K166 (= K188)
binding aspartic acid: T13 (= T35), G59 (= G81), S60 (= S82), E61 (= E83), G92 (= G114), T93 (= T115), D94 (= D116)

P06608 L-asparaginase; L-ASNase; L-asparagine amidohydrolase; EC 3.5.1.1 from Dickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi) (see 2 papers)
73% identity, 94% coverage: 23:347/347 of query aligns to 24:348/348 of P06608

query
sites
P06608

R

K

L

L

P

P

H

N

I

I

V

V

I

I

L

L

A

A

T

T

G

G

G

G

T
|
T

I

I

A

A

G

G

S

S

A

A

A

A

S

T

N

G

T

T

Q

Q

T

T

T

T

G

G

Y

Y

K

K

A

A

G

G

A

A

I

L

G

G

V

V

Q

D

T

T

L

L

I

I

N

N

A

A

V

V

P

P

E

E

M

V

S

K

K

K

I

L

A

A

H

N

V

V

E

K

G

G

E

E

Q

Q

V

F

A

S

N

N

I

M

G

A

S

S

E

E

N

N

M

M

T

T

S

G

D

D

I

V

I

V

L

L

E

K

L

L

S

S

K

Q

R

R

V

V

N

N

A

E

L

L

L

L

A

A

R

R

D

D

D

D

V

V

D

D

G

G

V

V

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V

I

I

T

T

H

H

G

G

T

T

D

D

T

T

L

V

D

E

E

E

T

S

P

A

Y

Y

F

F

L

L

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H

L

L

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T

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V

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K

S

S

N

D

K

K

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P

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F

F

T

V

A

A

A

A

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M

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R

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P

A

A

T

T

A

A

I

I

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S

A

A

D

D

G

G

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P

M

M

N

N

L

L

L

L

E

E

A

A

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R

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V

A

A

A

G

D

D

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K

D

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A

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R

G

G

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G

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V

M

M

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V

L

L

N

N

D

D

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R

I

I

G

G

A

S

A

A

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R

F

Y

V

I

T

T

K

K

T

T

N

N

A

A

T

S

S

T

L

L

D

D

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T

F

F

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K

A

A

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E

E

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E

G

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D

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L

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P

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D

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L

Y

Y

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G

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Y

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Q

D

D

D

D

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L

Y

Y

D

D

A

A

A

A

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Q

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K

G

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Y

A

A

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G

A

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A

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K

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S

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T

T

G

G

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N

G

G

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I

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V

P

P

P

P

D

D

D

E

S

E

Q

L

P

P

G

G

L

L

V

V

S

S

D

D

S

S

L

L

N

N

P

P

A

A

K

H

A

A

R

R

I

I

L

L

L

L

M

M

T

L

A

A

L

L

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R

T

T

K

S

D

D

P

P

Q

K

L

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I

I

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Q

Q

E

Y

Y

F

F

H

H

T

T

Y

Y

T36 (= T35) active site, O-isoaspartyl threonine intermediate

1hg0A X-ray structure of the complex between erwinia chrysanthemi l- asparaginase and succinic acid (see paper)
73% identity, 94% coverage: 23:347/347 of query aligns to 3:327/327 of 1hg0A

query
sites
1hg0A

R

K

L

L

P

P

H

N

I

I

V

V

I

I

L

L

A

A

T

T

G

G

G

G

T
|
T

I

I

A

A

G

G

S

S

A

A

A

A

S

T

N

G

T

T

Q

Q

T

T

T

T

G

G

Y
|
Y

K

K

A

A

G

G

A

A

I

L

G

G

V

V

Q

D

T

T

L

L

I

I

N

N

A

A

V

V

P

P

E

E

M

V

S

K

K

K

I

L

A

A

H

N

V

V

E

K

G

G

E

E

Q

Q

V

F

A

S

N

N

I

M

G
x
A

S
|
S

E

E

N

N

M

M

T

T

S

G

D

D

I

V

I

V

L

L

E

K

L

L

S

S

K

Q

R

R

V

V

N

N

A

E

L

L

L

L

A

A

R

R

D

D

D

D

V

V

D

D

G

G

V

V

V

V

I

I

T

T

H

H

G
|
G

T
|
T

D
|
D

T

T

L

V

D

E

E

E

T

S

P

A

Y

Y

F

F

L

L

N

H

L

L

T

T

V

V

K

K

S

S

N

D

K

K

P

P

V

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V

F

F

T

V

A

A

A

A

M

M

R

R

P

P

A

A

T

T

A

A

I

I

S

S

A

A

D

D

G

G

P

P

M

M

N

N

L

L

L

L

E

E

A

A

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V

T

R

V

V

A

A

A

G

D

D

P

K

D

Q

A

S

R

R

G

G

R

R

G

G

V

V

M

M

V

V

V

V

L

I

N

N

D

D

R

R

I

I

G

G

A

S

A

A

R

R

F

Y

V

I

T

T

K
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K

T

T

N

N

A

A

T

S

S

T

L

L

D

D

T

T

F

F

R

R

A

A

P

N

E

E

E

E

G

G

Y

Y

L

L

G

G

V

V

V

I

V

I

G

G

G

N

K

R

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Y

F

Y

E

Q

T

N

R

R

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I

D

D

K

K

I

L

H

H

T

T

L

T

R

R

S

S

V

V

F

F

D

D

V

V

R

R

Q

G

L

L

K

T

T

S

L

L

P

P

K

K

V

V

V

D

I

I

I

L

Y

Y

G

G

Y

Y

Q

Q

D

D

D

D

P

P

E

E

Y

Y

M

L

Y

Y

D

D

A

A

A

A

I

I

A

Q

H

H

H

G

A

V

D

K

G

G

I

I

I

V

Y

Y

A

A

G

G

T

M

G

G

A

A

G

G

S

S

V

V

S

S

V

V

R

R

S

G

A

I

A

A

G

G

I

M

K

R

K

K

A

A

Q

L

Q

E

A

K

G

G

I

V

V

V

V

V

V

M

R

R

A

S

S

T

R

R

T

T

G

G

S

N

G

G

V

I

V

V

P

P

P

P

D

D

D

E

S

E

Q

L

P

P

G

G

L

L

V

V

S

S

D

D

S

S

L

L

N

N

P

P

A

A

K

H

A

A

R

R

I

I

L

L

L

L

M

M

T

L

A

A

L

L

T

T

Q

R

T

T

K

S

D

D

P

P

Q

K

L

V

I

I

Q

Q

Q

E

Y

Y

F

F

H

H

T

T

Y

Y

active site: T15 (= T35), Y29 (= Y49), T95 (= T115), D96 (= D116), K168 (= K188)
binding succinic acid: T15 (= T35), A61 (≠ G81), S62 (= S82), G94 (= G114), T95 (= T115), D96 (= D116)

1jsrA Crystal structure of erwinia chrysanthemi l-asparaginase complexed with 6-hydroxy-l-norleucine (see paper)
73% identity, 93% coverage: 24:347/347 of query aligns to 1:324/324 of 1jsrA

query
sites
1jsrA

L

L

P

P

H

N

I

I

V

V

I

I

L

L

A

A

T

T

G

G

G

G

T
|
T

I

I

A

A

G

G

S

S

A

A

A

A

S

T

N

G

T

T

Q

Q

T

T

T

T

G

G

Y
|
Y

K

K

A

A

G

G

A

A

I

L

G

G

V

V

Q

D

T

T

L

L

I

I

N

N

A

A

V

V

P

P

E

E

M

V

S

K

K

K

I

L

A

A

H

N

V

V

E

K

G

G

E

E

Q

Q

V

F

A

S

N

N

I

M

G
x
A

S
|
S

E
|
E

N

N

M

M

T

T

S

G

D

D

I

V

I

V

L

L

E

K

L

L

S

S

K

Q

R

R

V

V

N

N

A

E

L

L

L

L

A

A

R

R

D

D

D

D

V

V

D

D

G

G

V

V

V

V

I

I

T

T

H

H

G
|
G

T
|
T

D
|
D

T

T

L

V

D

E

E

E

T

S

P

A

Y

Y

F

F

L

L

N

H

L

L

T

T

V

V

K

K

S

S

N

D

K

K

P

P

V

V

V

V

F

F

T

V

A

A

A

A

M

M

R

R

P

P

A

A

T

T

A

A

I

I

S

S

A

A

D

D

G

G

P

P

M

M

N

N

L

L

L

L

E

E

A

A

V

V

T

R

V

V

A

A

A

G

D

D

P

K

D

Q

A

S

R

R

G

G

R

R

G

G

V

V

M

M

V

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V

L

I

N

N

D

D

R

R

I

I

G

G

A

S

A

A

R

R

F

Y

V

I

T

T

K
|
K

T

T

N

N

A

A

T

S

S

T

L

L

D

D

T

T

F

F

R

R

A

A

P

N

E

E

E

E

G

G

Y

Y

L

L

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I

V

I

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G

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N

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D

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K

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F

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L

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P

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K

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V

D

I

I

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L

Y

Y

G

G

Y

Y

Q

Q

D

D

D

D

P

P

E

E

Y

Y

M

L

Y

Y

D

D

A

A

A

A

I

I

A

Q

H

H

H

G

A

V

D

K

G

G

I

I

I

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Y

Y

A

A

G

G

T

M

G

G

A

A

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G

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V

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R

S

G

A

I

A

A

G

G

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K

A

A

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L

Q

E

A

K

G

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V

M

R

R

A

S

S

T

R

R

T

T

G

G

S

N

G

G

V

I

V

V

P

P

P

P

D

D

D

E

S

E

Q

L

P

P

G

G

L

L

V

V

S

S

D

D

S

S

L

L

N

N

P

P

A

A

K

H

A

A

R

R

I

I

L

L

L

L

M

M

T

L

A

A

L

L

T

T

Q

R

T

T

K

S

D

D

P

P

Q

K

L

V

I

I

Q

Q

Q

E

Y

Y

F

F

H

H

T

T

Y

Y

active site: T12 (= T35), Y26 (= Y49), T92 (= T115), D93 (= D116), K165 (= K188)
binding 6-hydroxy-l-norleucine: T12 (= T35), Y26 (= Y49), A58 (≠ G81), S59 (= S82), E60 (= E83), G91 (= G114), T92 (= T115), D93 (= D116)

1jslA Crystal structure of erwinia chrysanthemi l-asparaginase complexed with 6-hydroxy-d-norleucine (see paper)
73% identity, 93% coverage: 24:347/347 of query aligns to 1:324/324 of 1jslA

query
sites
1jslA

L

L

P

P

H

N

I

I

V

V

I

I

L

L

A

A

T

T

G

G

G

G

T
|
T

I

I

A

A

G

G

S

S

A

A

A

A

S

T

N

G

T

T

Q

Q

T

T

T

T

G

G

Y
|
Y

K

K

A

A

G

G

A

A

I

L

G

G

V

V

Q

D

T

T

L

L

I

I

N

N

A

A

V

V

P

P

E

E

M

V

S

K

K

K

I

L

A

A

H

N

V

V

E

K

G

G

E

E

Q

Q

V

F

A

S

N

N

I

M

G
x
A

S
|
S

E
|
E

N

N

M

M

T

T

S

G

D

D

I

V

I

V

L

L

E

K

L

L

S

S

K

Q

R

R

V

V

N

N

A

E

L

L

L

L

A

A

R

R

D

D

D

D

V

V

D

D

G

G

V

V

V

V

I

I

T

T

H

H

G
|
G

T
|
T

D
|
D

T

T

L

V

D

E

E

E

T

S

P

A

Y

Y

F

F

L

L

N

H

L

L

T

T

V

V

K

K

S

S

N

D

K

K

P

P

V

V

V

V

F

F

T

V

A

A

A
|
A

M

M

R

R

P

P

A

A

T

T

A

A

I

I

S

S

A

A

D

D

G

G

P

P

M

M

N

N

L

L

L

L

E

E

A

A

V

V

T

R

V

V

A

A

A

G

D

D

P

K

D

Q

A

S

R

R

G

G

R

R

G

G

V

V

M

M

V

V

V

V

L

I

N

N

D

D

R

R

I

I

G

G

A

S

A

A

R

R

F

Y

V

I

T

T

K
|
K

T

T

N

N

A

A

T

S

S

T

L

L

D

D

T

T

F

F

R

R

A

A

P

N

E

E

E

E

G

G

Y

Y

L

L

G

G

V

V

V

I

V

I

G

G

G

N

K

R

P

I

Q

Y

F

Y

E

Q

T

N

R

R

V

I

D

D

K

K

I

L

H

H

T

T

L

T

R

R

S

S

V

V

F

F

D

D

V

V

R

R

Q

G

L

L

K

T

T

S

L

L

P

P

K

K

V

V

V

D

I

I

I

L

Y

Y

G

G

Y

Y

Q

Q

D

D

D

D

P

P

E

E

Y

Y

M

L

Y

Y

D

D

A

A

A

A

I

I

A

Q

H

H

H

G

A

V

D

K

G

G

I

I

I

V

Y

Y

A

A

G

G

T

M

G

G

A

A

G

G

S

S

V

V

S

S

V

V

R

R

S

G

A

I

A

A

G

G

I

M

K

R

K

K

A

A

Q

L

Q

E

A

K

G

G

I

V

V

V

V

V

V

M

R

R

A

S

S

T

R

R

T

T

G

G

S

N

G

G

V

I

V

V

P

P

P

P

D

D

D

E

S

E

Q

L

P

P

G

G

L

L

V

V

S

S

D

D

S

S

L

L

N

N

P

P

A

A

K

H

A

A

R

R

I

I

L

L

L

L

M

M

T

L

A

A

L

L

T

T

Q

R

T

T

K

S

D

D

P

P

Q

K

L

V

I

I

Q

Q

Q

E

Y

Y

F

F

H

H

T

T

Y

Y

active site: T12 (= T35), Y26 (= Y49), T92 (= T115), D93 (= D116), K165 (= K188)
binding 6-hydroxy-d-norleucine: T12 (= T35), Y26 (= Y49), A58 (≠ G81), S59 (= S82), E60 (= E83), G91 (= G114), T92 (= T115), D93 (= D116), A117 (= A140)

7u6mC Albumin binding domain fused to a mutant of the erwinia asparaginase (see paper)
70% identity, 98% coverage: 9:347/347 of query aligns to 4:342/342 of 7u6mC

query
sites
7u6mC

I

I

A

C

L

L

L

P

T

R

M

W

S

G

S

C

L

L

A

W

F

E

S

D

E

D

T

D

R

K

L

L

P

P

H

N

I

I

V

V

I

I

L

L

A

A

T

T

G

G

G

G

T
|
T

I

I

A

A

G

G

S

S

A

A

A

A

S

T

N

G

T

T

Q

Q

T

T

T

T

G

G

Y

Y

K

K

A

I

G

G

A

A

I

L

G

G

V

V

Q

D

T

T

L

L

I

I

N

N

A

A

V

V

P

P

E

E

M

V

S

K

K

K

I

L

A

A

H

N

V

V

E

K

G

G

E

E

Q

Q

V

F

A

S

N

N

I

M

G
x
A

S
|
S

E
x
Q

N

N

M

M

T

T

S

G

D

D

I

V

I

V

L

L

E

K

L

L

S

S

K

Q

R

R

V

V

N

N

A

E

L

L

L

L

A

A

R

R

D

D

D

D

V

V

D

D

G

G

V

V

V

V

I

I

T

T

H

H

G
|
G

T
|
T

D
|
D

T

T

L

V

D

E

E

E

T

S

P

A

Y

Y

F

F

L

L

N

H

L

L

T

T

V

V

K

K

S

S

N

D

K

K

P

P

V

V

V

V

F

F

T

V

A

A

A

A

M

M

R

R

P

P

A

A

T

T

A

A

I

I

S

S

A

A

D

D

G

G

P

P

M

M

N

N

L

L

L

L

E

E

A

A

V

V

T

R

V

V

A

A

A

G

D

D

P

K

D

Q

A

S

R

R

G

G

R

R

G

G

V

V

M

M

V

V

V

V

L

L

N

N

D

D

R

R

I

I

G

G

A

S

A

A

R

R

F

Y

V

I

T

T

K

K

T

T

N

N

A

A

T

S

S

T

L

L

D

D

T

T

F

F

R

K

A

A

P

N

E

E

E

E

G

G

Y

Y

L

L

G

G

V

V

V

I

V

I

G

G

G

N

K

R

P

I

Q

Y

F

Y

E

Q

T

N

R

R

V

I

D

D

K

K

I

L

H

H

T

T

L

T

R

R

S

S

V

V

F

F

D

D

V

V

R

R

Q

G

L

L

K

T

T

S

L

L

P

P

K

K

V

V

V

D

I

I

I

L

Y

Y

G

G

Y

Y

Q

Q

D

D

D

D

P

P

E

E

Y

Y

M

L

Y

Y

D

D

A

A

A

A

I

I

A

Q

H

H

H

G

A

V

D

K

G

G

I

I

I

V

Y

Y

A

A

G

G

T

M

G

G

A

A

G

G

S

Q

V

V

S

S

V

V

R

R

S

G

A

I

A

A

G

G

I

M

K

R

K

K

A

A

Q

M

Q

E

A

K

G

G

I

V

V

V

V

V

V

I

R

R

A

S

S

T

R

R

T

T

G

G

S

N

G

G

V

I

V

V

P

P

P

P

D

D

D

E

S

E

Q

L

P

P

G

G

L

L

V

V

S

S

D

D

S

S

L

L

N

N

P

P

A

A

K

H

A

A

R

R

I

I

L

L

L

L

M

M

T

L

A

A

L

L

T

T

Q

R

T

T

K

S

D

D

P

P

Q

K

L

V

I

I

Q

Q

Q

E

Y

Y

F

F

H

H

T

T

Y

Y

binding aspartic acid: T30 (= T35), A76 (≠ G81), S77 (= S82), Q78 (≠ E83), G109 (= G114), T110 (= T115), D111 (= D116)

2hlnA L-asparaginase from erwinia carotovora in complex with glutamic acid (see paper)
71% identity, 93% coverage: 24:347/347 of query aligns to 2:308/308 of 2hlnA

query
sites
2hlnA

L

L

P

P

H

N

I

I

V

V

I

I

L

L

A

A

T

T

G

G

G
|
G

T
|
T

I

I

A

A

G

G

S

-

A

-

A

-

S

-

N

-

T

-

Q

-

T

-

T

-

G

-

Y

-

K

-

A

-

G

-

A

-

I

-

G

-

V

V

Q

E

T

T

L

L

I

I

N

Q

A

A

V

V

P

P

E

E

M

L

S

K

K

T

I

L

A

A

H

N

V

I

E

K

G

G

E

E

Q

Q

V

V

A

A

N

S

I

I

G
|
G

S
|
S

E
|
E

N

N

M

M

T

T

S

S

D

D

I

V

I

L

L

L

E

T

L

L

S

S

K

K

R

R

V

V

N

N

A

E

L

L

L

L

A

A

R

R

D

S

D

D

V

V

D

D

G

G

V

V

V

V

I

I

T

T

H

H

G
|
G

T
|
T

D
|
D

T

T

L

L

D

D

E

E

T

S

P

P

Y

Y

F

F

L

L

N

N

L

L

T

T

V

V

K

K

S

S

N

D

K

K

P

P

V

V

V

V

F

F

T

V

A

A

A

A

M

M

R

R

P

P

A

A

T

T

A

A

I

I

S

S

A

A

D

D

G

G

P

P

M

M

N

N

L

L

L

Y

E

G

A

A

V

V

T

K

V

V

A

A

A

A

D

D

P

K

D

N

A

S

R

R

G

G

R

R

G

G

V

V

M

L

V

V

V

V

L

L

N

N

D

D

R

R

I

I

G

G

A

S

A

A

R

R

F

F

V

I

T

S

K
|
K

T

T

N

N

A

A

T

S

S

T

L

L

D

D

T

T

F

F

R

K

A

A

P

P

E

E

E

E

G

G

Y

Y

L

L

G

G

V

V

V

I

V

I

G

G

G

D

K

K

P

I

Q

Y

F

Y

E

Q

T

T

R

R

V

L

D

D

K

K

I

V

H

H

T

T

L

T

R

R

S

S

V

V

F

F

D

D

V

V

R

T

Q

N

L

V

K

D

T

K

L

L

P

P

K

A

V

V

V

D

I

I

I

I

Y

Y

G

G

Y

Y

Q

Q

D

D

D

D

P

P

E

E

Y

Y

M

M

Y

Y

D

D

A

A

A

S

I

I

A

K

H

H

H

G

A

V

D

K

G

G

I

I

I

V

Y

Y

A

A

G

G

T

M

G

G

A

A

G

G

S

S

V

V

S

S

V

K

R

R

S

G

A

D

A

A

G

G

I

I

K

R

K

K

A

A

Q

E

Q

S

A

K

G

G

I

I

V

V

V

V

V

V

R

R

A

S

S

S

R

R

T

T

G

G

S

S

G

G

V

I

V

V

P

P

P

P

D

D

D

A

S

G

Q

Q

P

P

G

G

L

L

V

V

S

A

D

D

S

S

L

L

N

S

P

P

A

A

K

K

A

S

R

R

I

I

L

L

L

L

M

M

T

L

A

A

L

L

T

T

Q

K

T

T

K

T

D

N

P

P

Q

A

L

V

I

I

Q

Q

Q

D

Y

Y

F

F

H

H

T

A

Y

Y

active site: T13 (= T35), T76 (= T115), D77 (= D116), K149 (= K188)
binding glutamic acid: G12 (= G34), T13 (= T35), G42 (= G81), S43 (= S82), E44 (= E83), G75 (= G114), T76 (= T115), D77 (= D116)

1hfwA X-ray structure of the complex between erwinia chrysanthemi l- asparaginase and l-glutamate (see paper)
69% identity, 94% coverage: 23:347/347 of query aligns to 1:307/307 of 1hfwA

query
sites
1hfwA

R

K

L

L

P

P

H

N

I

I

V

V

I

I

L

L

A

A

T

T

G

G

G
|
G

T
|
T

I

I

A

A

G

-

S

-

A

-

A

-

S

-

N

-

T

-

Q

-

T

-

T

-

G

-

Y

-

K

-

A

-

G

-

A

-

I

-

G

-

V

V

Q

D

T

T

L

L

I

I

N

N

A

A

V

V

P

P

E

E

M

V

S

K

K

K

I

L

A

A

H

N

V

V

E

K

G

G

E

E

Q

Q

V

F

A

S

N

N

I

M

G

A

S
|
S

E
|
E

N

N

M

M

T

T

S

G

D

D

I

V

I

V

L

L

E

K

L

L

S

S

K

Q

R

R

V

V

N

N

A

E

L

L

L

L

A

A

R

R

D

D

D

D

V

V

D

D

G

G

V

V

V

V

I

I

T

T

H

H

G
|
G

T
|
T

D
|
D

T

T

L

V

D

E

E

E

T

S

P

A

Y

Y

F

F

L

L

N

H

L

L

T

T

V

V

K

K

S

S

N

D

K

K

P

P

V

V

V

V

F

F

T

V

A

A

A

A

M

M

R

R

P

P

A

A

T

T

A

A

I

I

S

S

A

A

D

D

G

G

P

P

M

M

N

N

L

L

L

L

E

E

A

A

V

V

T

R

V

V

A

A

A

G

D

D

P

K

D

Q

A

S

R

R

G

G

R

R

G

G

V

V

M

M

V

V

V

V

L

I

N

N

D

D

R

R

I

I

G

G

A

S

A

A

R

R

F

Y

V

I

T

T

K
|
K

T

T

N

N

A

A

T

S

S

T

L

L

D

D

T

T

F

F

R

R

A

A

P

N

E

E

E

E

G

G

Y

Y

L

L

G

G

V

V

V

I

V

I

G

G

G

N

K

R

P

I

Q

Y

F

Y

E

Q

T

N

R

R

V

I

D

D

K

K

I

L

H

H

T

T

L

T

R

R

S

S

V

V

F

F

D

D

V

V

R

R

Q

G

L

L

K

T

T

S

L

L

P

P

K

K

V

V

V

D

I

I

I

L

Y

Y

G

G

Y

Y

Q

Q

D

D

D

D

P

P

E

E

Y

Y

M

L

Y

Y

D

D

A

A

A

A

I

I

A

Q

H

H

H

G

A

V

D

K

G

G

I

I

I

V

Y

Y

A

A

G

G

T

M

G

G

A

A

G

G

S

S

V

V

S

S

V

V

R

R

S

G

A

I

A

A

G

G

I

M

K

R

K

K

A

A

Q

L

Q

E

A

K

G

G

I

V

V

V

V

V

V

M

R

R

A

S

S

T

R

R

T

T

G

G

S

N

G

G

V

I

V

V

P

P

P

P

D

D

D

E

S

E

Q

L

P

P

G

G

L

L

V

V

S

S

D

D

S

S

L

L

N

N

P

P

A

A

K

H

A

A

R

R

I

I

L

L

L

L

M

M

T

L

A

A

L

L

T

T

Q

R

T

T

K

S

D

D

P

P

Q

K

L

V

I

I

Q

Q

Q

E

Y

Y

F

F

H

H

T

T

Y

Y

active site: T13 (= T35), T75 (= T115), D76 (= D116), K148 (= K188)
binding glutamic acid: G12 (= G34), T13 (= T35), S42 (= S82), E43 (= E83), G74 (= G114), T75 (= T115), D76 (= D116)

1hg1A X-ray structure of the complex between erwinia chrysanthemi l- asparaginase and d-aspartate (see paper)
69% identity, 93% coverage: 24:347/347 of query aligns to 1:306/306 of 1hg1A

query
sites
1hg1A

L

L

P

P

H

N

I

I

V

V

I

I

L

L

A

A

T

T

G

G

G
|
G

T
|
T

I

I

A

-

G

-

S

-

A

-

A

-

S

-

N

-

T

-

Q

-

T

-

T

-

G

-

Y

-

K

-

A

-

G

-

A

-

I

-

G

G

V

V

Q

D

T

T

L

L

I

I

N

N

A

A

V

V

P

P

E

E

M

V

S

K

K

K

I

L

A

A

H

N

V

V

E

K

G

G

E

E

Q

Q

V

F

A

S

N

N

I

M

G
x
A

S
|
S

E
|
E

N

N

M

M

T

T

S

G

D

D

I

V

I

V

L

L

E

K

L

L

S

S

K

Q

R

R

V

V

N

N

A

E

L

L

L

L

A

A

R

R

D

D

D

D

V

V

D

D

G

G

V

V

V

V

I

I

T

T

H

H

G
|
G

T
|
T

D
|
D

T

T

L

V

D

E

E

E

T

S

P

A

Y

Y

F

F

L

L

N

H

L

L

T

T

V

V

K

K

S

S

N

D

K

K

P

P

V

V

V

V

F

F

T

V

A

A

A

A

M

M

R

R

P

P

A

A

T

T

A

A

I

I

S

S

A

A

D

D

G

G

P

P

M

M

N

N

L

L

L

L

E

E

A

A

V

V

T

R

V

V

A

A

A

G

D

D

P

K

D

Q

A

S

R

R

G

G

R

R

G

G

V

V

M

M

V

V

V

V

L

I

N

N

D

D

R

R

I

I

G

G

A

S

A

A

R

R

F

Y

V

I

T

T

K
|
K

T

T

N

N

A

A

T

S

S

T

L

L

D

D

T

T

F

F

R

R

A

A

P

N

E

E

E

E

G

G

Y

Y

L

L

G

G

V

V

V

I

V

I

G

G

G

N

K

R

P

I

Q

Y

F

Y

E

Q

T

N

R

R

V

I

D

D

K

K

I

L

H

H

T

T

L

T

R

R

S

S

V

V

F

F

D

D

V

V

R

R

Q

G

L

L

K

T

T

S

L

L

P

P

K

K

V

V

V

D

I

I

I

L

Y

Y

G

G

Y

Y

Q

Q

D

D

D

D

P

P

E

E

Y

Y

M

L

Y

Y

D

D

A

A

A

A

I

I

A

Q

H

H

H

G

A

V

D

K

G

G

I

I

I

V

Y

Y

A

A

G

G

T

M

G

G

A

A

G

G

S

S

V

V

S

S

V

V

R

R

S

G

A

I

A

A

G

G

I

M

K

R

K

K

A

A

Q

L

Q

E

A

K

G

G

I

V

V

V

V

V

V

M

R

R

A

S

S

T

R

R

T

T

G

G

S

N

G

G

V

I

V

V

P

P

P

P

D

D

D

E

S

E

Q

L

P

P

G

G

L

L

V

V

S

S

D

D

S

S

L

L

N

N

P

P

A

A

K

H

A

A

R

R

I

I

L

L

L

L

M

M

T

L

A

A

L

L

T

T

Q

R

T

T

K

S

D

D

P

P

Q

K

L

V

I

I

Q

Q

Q

E

Y

Y

F

F

H

H

T

T

Y

Y

active site: T12 (= T35), T74 (= T115), D75 (= D116), K147 (= K188)
binding d-aspartic acid: G11 (= G34), T12 (= T35), A40 (≠ G81), S41 (= S82), E42 (= E83), G73 (= G114), T74 (= T115), D75 (= D116)

5k45A Wolinella succinogenes l-asparaginase p121 + l-glutamic acid (see paper)
48% identity, 93% coverage: 25:347/347 of query aligns to 2:328/328 of 5k45A

query
sites
5k45A

P

P

H

Q

I

V

V

T

I

I

L

L

A

A

T

T

G

G

G

G

T
|
T

I

I

A

A

G

G

S

S

A

G

A

E

S

S

N

S

T

V

Q

K

T

S

T

S

G

-

Y
|
Y

K

S

A

A

G

G

A

A

I

V

G

T

V

V

Q

D

T

K

L

L

I

L

N

A

A

A

V

V

P

P

E

A

M

I

S

N

K

D

I

L

A

A

H

T

V

I

E

K

G

G

E

E

Q

Q

V

I

A

S

N

S

I

I

G
|
G

S
|
S

E
x
Q

N

E

M

M

T

T

S

G

D

K

I

V

I

W

L

L

E

K

L

L

S

A

K

K

R

R

V

V

N

N

A

E

L

L

L

L

A

A

R

Q

D

K

D

E

V

T

D

E

G

A

V

V

V

I

I

I

T

T

H

H

G
|
G

T
|
T

D
|
D

T

T

L

M

D

E

E

E

T

T

P

A

Y

F

F

F

L

L

N

N

L

L

T

T

V

V

K

K

S

S

N

Q

K

K

P

P

V

V

V

V

F

L

T

V

A

G

A
|
A

M

M

R

R

P

P

A

G

T

S

A

S

I

M

S

S

A

A

D

D

G

G

P

P

M

M

N

N

L

L

L

Y

E

N

A

A

V

V

T

N

V

V

A

A

A

I

D

N

P

K

D

A

A

S

R

T

G

N

R

K

G

G

V

V

M

V

V

I

V

V

L

M

N

N

D

D

R

E

I

I

G

H

A

A

A

A

R

R

F

E

V

A

T

T

K
|
K

T

L

N

N

A

T

T

T

S

A

L

V

D

N

T

A

F

F

R

A

A

S

P

P

E

N

E

T

G

G

Y

K

L

I

G

G

V

T

V

V

V

Y

G

Y

G

G

K

K

P

V

Q

E

F

Y

E

F

T

T

R

Q

V

S

D

V

K

R

I

P

H

H

T

T

L

L

R

A

S

S

V

E

F

F

D

D

V

I

R

S

Q

K

L

I

K

E

T

E

L

L

P

P

K

R

V

V

V

D

I

I

I

L

Y

Y

G

A

Y

H

Q

P

D

D

D

D

P

T

E

D

Y

V

M

L

Y

V

D

N

A

A

A

A

I

L

A

Q

H

A

H

G

A

A

D

K

G

G

I

I

I

I

Y

H

A

A

G

G

T

M

G

G

A

N

G

G

S

N

V

P

S

F

V

P

R

L

S

T

A

Q

A

N

G

A

I

L

K

E

K

K

A

A

Q

A

Q

K

A

S

G

G

I

V

V

V

V

V

V

A

R

R

A

S

S

S

R

R

T

V

G

G

S

S

G

G

V

S

V

T

P

T

P

Q

-

E

-

A

-
x
E

-

V

D

D

D

D

S

K

Q

K

P

L

G

G

L

F

V

V

-

A

S

T

D

E

S

S

L

L

N

N

P

P

A

Q

K

K

A

A

R

R

I

V

L

L

L

L

M

M

T

L

A

A

L

L

T

T

Q

K

T

T

K

S

D

D

P

R

Q

E

L

A

I

I

Q

Q

Q

K

Y

I

F

F

H

S

T

T

Y

Y

active site: T12 (= T35), Y25 (= Y49), T91 (= T115), D92 (= D116), K164 (= K188), E285 (vs. gap)
binding glutamic acid: G57 (= G81), S58 (= S82), Q59 (≠ E83), G90 (= G114), T91 (= T115), D92 (= D116), A116 (= A140)

5k3oA Wolinella succinogenes l-asparaginase p121 and l-aspartic acid (see paper)
48% identity, 93% coverage: 25:347/347 of query aligns to 2:328/328 of 5k3oA

query
sites
5k3oA

P

P

H

Q

I

V

V

T

I

I

L

L

A

A

T

T

G

G

G

G

T
|
T

I

I

A

A

G

G

S

S

A

G

A

E

S

S

N

S

T

V

Q

K

T

S

T

S

G

-

Y
|
Y

K

S

A

A

G

G

A

A

I

V

G

T

V

V

Q

D

T

K

L

L

I

L

N

A

A

A

V

V

P

P

E

A

M

I

S

N

K

D

I

L

A

A

H

T

V

I

E

K

G

G

E

E

Q

Q

V

I

A

S

N

S

I

I

G
|
G

S
|
S

E
x
Q

N

E

M

M

T

T

S

G

D

K

I

V

I

W

L

L

E

K

L

L

S

A

K

K

R

R

V

V

N

N

A

E

L

L

L

L

A

A

R

Q

D

K

D

E

V

T

D

E

G

A

V

V

V

I

I

I

T

T

H

H

G
|
G

T
|
T

D
|
D

T

T

L

M

D

E

E

E

T

T

P

A

Y

F

F

F

L

L

N

N

L

L

T

T

V

V

K

K

S

S

N

Q

K

K

P

P

V

V

V

V

F

L

T

V

A

G

A

A

M

M

R

R

P

P

A

G

T

S

A

S

I

M

S

S

A

A

D

D

G

G

P

P

M

M

N

N

L

L

L

Y

E

N

A

A

V

V

T

N

V

V

A

A

A

I

D

N

P

K

D

A

A

S

R

T

G

N

R

K

G

G

V

V

M

V

V

I

V

V

L

M

N

N

D

D

R

E

I

I

G

H

A

A

A

A

R

R

F

E

V

A

T

T

K
|
K

T

L

N

N

A

T

T

T

S

A

L

V

D

N

T

A

F

F

R

A

A

S

P

P

E

N

E

T

G

G

Y

K

L

I

G

G

V

T

V

V

V

Y

G

Y

G

G

K

K

P

V

Q

E

F

Y

E

F

T

T

R

Q

V

S

D

V

K

R

I

P

H

H

T

T

L

L

R

A

S

S

V

E

F

F

D

D

V

I

R

S

Q

K

L

I

K

E

T

E

L

L

P

P

K

R

V

V

V

D

I

I

I

L

Y

Y

G

A

Y

H

Q

P

D

D

D

D

P

T

E

D

Y

V

M

L

Y

V

D

N

A

A

A

A

I

L

A

Q

H

A

H

G

A

A

D

K

G

G

I

I

I

I

Y

H

A

A

G

G

T

M

G

G

A

N

G

G

S

N

V

P

S

F

V

P

R

L

S

T

A

Q

A

N

G

A

I

L

K

E

K

K

A

A

Q

A

Q

K

A

S

G

G

I

V

V

V

V

V

V

A

R

R

A

S

S

S

R

R

T

V

G

G

S

S

G

G

V

S

V

T

P

T

P

Q

-

E

-

A

-
x
E

-

V

D

D

D

D

S

K

Q

K

P

L

G

G

L

F

V

V

-

A

S

T

D

E

S

S

L

L

N

N

P

P

A

Q

K

K

A

A

R

R

I

V

L

L

L

L

M

M

T

L

A

A

L

L

T

T

Q

K

T

T

K

S

D

D

P

R

Q

E

L

A

I

I

Q

Q

Q

K

Y

I

F

F

H

S

T

T

Y

Y

active site: T12 (= T35), Y25 (= Y49), T91 (= T115), D92 (= D116), K164 (= K188), E285 (vs. gap)
binding aspartic acid: T12 (= T35), G57 (= G81), S58 (= S82), Q59 (≠ E83), G90 (= G114), T91 (= T115), D92 (= D116)

2wltA The crystal structure of helicobacter pylori l-asparaginase at 1.4 a resolution (see paper)
47% identity, 93% coverage: 24:347/347 of query aligns to 1:326/326 of 2wltA

query
sites
2wltA

L

L

P

P

H

T

I

I

V

A

I

L

L

L

A

A

T

T

G

G

G

G

T
|
T

I

I

A

A

G

G

S

S

A

G

A

A

S

S

N

-

T

-

Q

-

T

L

T

G

G

S

Y
|
Y

K

K

A
x
S

G

G

A

E

I

L

G

G

V

V

Q

K

T

E

L

L

I

L

N

K

A

A

V

I

P

P

E

S

M

L

S

N

K

K

I

I

A

A

H

R

V

I

E

Q

G

G

E

E

Q

Q

V

V

A

S

N

N

I

I

G
|
G

S
|
S

E
x
Q

N

D

M

M

T

N

S

E

D

E

I

I

I

W

L

F

E

K

L

L

S

A

K

Q

R

R

V

A

N

Q

A

E

L

L

L

L

A

D

R

D

D

S

D

R

V

I

D

Q

G

G

V

V

V

V

I

I

T

T

H

H

G
|
G

T
|
T

D
|
D

T

T

L

L

D

E

E

E

T

S

P

A

Y

Y

F

F

L

L

N

N

L

L

T

V

V

L

K

H

S

S

N

T

K

K

P

P

V

V

V

V

F

L

T

V

A

G

A

A

M

M

R

R

P

N

A

A

T

S

A

S

I

L

S

S

A

A

D

D

G

G

P

A

M

L

N

N

L

L

L

Y

E

E

A

A

V

V

T

S

V

V

A

A

A

V

D

N

P

E

D

K

A

S

R

A

G

N

R

K

G

G

V

V

M

L

V

V

V

V

L

M

N

D

D

D

R

T

I

I

G

F

A

S

A

V

R

R

F

E

V

V

T

V

K
|
K

T

T

N

H

A

T

T

T

S

H

L

V

D

S

T

T

F

F

R

K

A

A

P

L

E

N

E

S

G

G

Y

A

L

I

G

G

V

S

V

V

V

Y

G

Y

G

G

K

K

P

T

Q

R

F

Y

E

Y

T

M

R

Q

V

P

D

L

K

R

I

K

H

H

T

T

L

T

R

E

S

S

V

E

F

F

D

S

V

L

R

S

Q

Q

L

L

K

K

T

T

-

P

L

L

P

P

K

K

V

V

V

D

I

I

I

I

Y

Y

G

T

Y

H

Q

A

D

G

D

M

P

T

E

P

Y

D

M

L

Y

F

D

Q

A

A

A

S

I

L

A

N

H

S

H

H

A

A

D

K

G

G

I

V

I

V

Y

I

A

A

G

G

T

V

G

G

A

N

G

G

S

N

V

V

S

S

V

A

R

G

S

F

A

L

A

K

G

A

I

M

K

Q

K

E

A

A

Q

S

Q

Q

A

M

G

G

I

V

V

V

V

I

V

V

R

R

A

S

S

S

R

R

T

V

G

G

S

S

G

G

V

G

V

V

P

T

P

S

-

G

-
x
E

-

I

D

D

D

D

S

K

Q

A

P

Y

G

G

L

F

V

I

-

T

S

S

D

D

S

N

L

L

N

N

P

P

A

Q

K

K

A

A

R

R

I

V

L

L

L

L

M

Q

T

L

A

A

L

L

T

T

Q

K

T

T

K

N

D

D

P

K

Q

A

L

K

I

I

Q

Q

Q

E

Y

M

F

F

H

E

T

E

Y

Y

active site: T12 (= T35), Y23 (= Y49), T89 (= T115), D90 (= D116), K162 (= K188), E283 (vs. gap)
binding aspartic acid: T12 (= T35), S25 (≠ A51), G55 (= G81), S56 (= S82), Q57 (≠ E83), G88 (= G114), T89 (= T115), D90 (= D116)

P00805 L-asparaginase 2; L-asparaginase II; L-ASNase II; L-asparagine amidohydrolase II; Colaspase; EC 3.5.1.1 from Escherichia coli (strain K12) (see 5 papers)
46% identity, 100% coverage: 1:347/347 of query aligns to 1:348/348 of P00805

query
sites
P00805

M
|
M

N
x
E

F
|
F

R
x
F

A
x
K

L
x
K

L
x
T

T
x
A

I
x
L

A
|
A

L
x
A

L
|
L

T
x
V

M
|
M

S
x
G

S

-

L
x
F

A
x
S

F
x
G

S
x
A

E
x
A

T
x
L

R
x
A

L

L

P

P

H

N

I

I

V

T

I

I

L

L

A

A

T

T

G

G

G

G

T
|
T

I

I

A

A

G

G

S

G

A

G

A

D

S

S

N

A

T

T

Q

K

T

S

T

N

G

-

Y

Y

K

T

A

V

G

G

A

K

I

V

G

G

V

V

Q

E

T

N

L

L

I

V

N

N

A

A

V

V

P

P

E

Q

M

L

S

K

K

D

I

I

A

A

H

N

V

V

E

K

G

G

E

E

Q

Q

V

V

A

V

N

N

I

I

G

G

S
|
S

E
x
Q

N

D

M

M

T

N

S

D

D

N

I

V

I

W

L

L

E

T

L

L

S

A

K

K

R

K

V

I

N

N

A

T

L

D

L
x
C

A

-

R

-

D

D

D

K

V

T

D

D

G

G

V

F

V

V

I

I

T

T

H

H

G

G

T
|
T

D
|
D

T

T

L

M

D

E

E

E

T

T

P

A

Y

Y

F

F

L

L

N

D

L

L

T

T

V

V

K

K

S
x
C

N

D

K

K

P

P

V

V

V

V

F

M

T

V

A

G

A

A

M

M

R

R

P

P

A

S

T

T

A

S

I

M

S

S

A

A

D

D

G

G

P

P

M

F

N

N

L

L

L

Y

E

N

A

A

V

V

T

V

V

T

A

A

A

A

D

D

P

K

D

A

A

S

R

A

G

N

R

R

G

G

V

V

M

L

V

V

V

V

L

M

N

N

D

D

R

T

I

V

G

L

A

D

A

G

R

R

F

D

V

V

T

T

K

K

T

T

N

N

A

T

T

T

S

D

L

V

D

A

T

T

F

F

R

K

A

S

P

V

E

N

E

Y

G

G

Y

P

L

L

G

G

V

Y

V

I

V

H

G

N

G

G

K

K

P

I

Q

D

F

Y

E

Q

T

R

R

T

V

P

D

A

K

R

I

K

H

H

T

T

L

S

R

D

S

T

V

P

F

F

D

D

V

V

R

S

Q

K

L

L

K

N

T

E

L

L

P

P

K

K

V

V

V

G

I

I

I

V

Y

Y

G

N

Y

Y

Q

A

D

N

D

A

P

S

E

D

Y

L

M

P

Y

A

D

K

A

A

A

L

I

V

A

D

H

A

H

G

A

Y

D

D

G

G

I

I

I

V

Y

S

A

A

G

G

T

V

G

G

A

N

G

G

S

N

V

L

S

Y

V

K

R

S

S

V

A

F

A

D

G

T

I

L

K

A

K

T

A

A

Q

A

Q

K

A

T

G

G

I

T

V

A

V

V

V

V

R

R

A

S

S

S

R

R

T

V

G

P

S

T

G

G

V

A

V

T

P

T

P

Q

-

D

-

A

-

E

-

V

D

D

D

D

S

A

Q

K

P

Y

G

G

L

F

V

V

-

A

S

S

D

G

S

T

L

L

N

N

P

P

A

Q

K

K

A

A

R

R

I

V

L

L

L

L

M

Q

T

L

A

A

L

L

T

T

Q

Q

T

T

K

K

D

D

P

P

Q

Q

L

Q

I

I

Q

Q

Q

Q

Y

I

F

F

H

N

T

Q

Y

Y

1:22 (vs. 1:23, 22% identical) signal peptide
T34 (= T35) active site, O-isoaspartyl threonine intermediate; mutation to A: Loss of enzyme activity.
SQ 80:81 (≠ SE 82:83) binding substrate
C99 (≠ L101) modified: Disulfide link with 127
T111 (= T115) mutation to S: Reduced enzyme activity.; mutation to V: Loss of enzyme activity.
TD 111:112 (= TD 115:116) binding substrate
C127 (≠ S131) modified: Disulfide link with 99

6wywA Crystal structure of pseudomonas 7a glutaminase-asparaginase in complex with l-asp at ph 4.5
44% identity, 93% coverage: 24:347/347 of query aligns to 1:317/317 of 6wywA

query
sites
6wywA

L

L

P

A

H

N

I

V

V

V

I

I

L

L

A

A

T

T

G

G

G
|
G

T
|
T

I

I

A

A

G

G

S

A

A

A

A

K

S

-

N

-

T

-

Q

-

T

-

T

-

G

-

Y

-

K

-

A

-

G

-

A

-

I

L

G

G

V

V

Q

D

T

K

L

L

I

I

N

A

A

G

V

V

P

P

E

E

M

L

S

A

K

D

I

I

A

A

H

N

V

V

E

R

G

G

E

E

Q

Q

V

V

A

M

N

Q

I

I

G
x
A

S
|
S

E
|
E

N

S

M

I

T

S

S

N

D

D

I

D

I

L

L

L

E

K

L

L

S

G

K

K

R

R

V

V

N

A

A

E

L

L

L

A

A

E

R

S

D

K

D

D

V

V

D

D

G

G

V

I

V

V

I

I

T

T

H

H

G
|
G

T
|
T

D
|
D

T

T

L

L

D

E

E

E

T

T

P

A

Y

F

F

F

L

L

N

N

L

L

T

V

V

E

K

K

S

T

N

D

K

K

P

P

V

I

V

V

F

V

T

V

A

G

A

S

M

M

R

R

P

P

A

G

T

T

A

A

I

M

S

S

A

A

D

D

G

G

P

M

M

L

N

N

L

L

L

Y

E

N

A

A

V

V

T

A

V

V

A

A

A

S

D

D

P

K

D

Q

A

S

R

R

G

G

R

K

G

G

V

V

M

L

V

V

V

T

L

M

N

N

D

D

R

E

I

I

G

Q

A

S

A

G

R

R

F

D

V

V

T

S

K
|
K

T

A

N

V

A

N

T

I

S

K

L

T

D

E

T

A

F

F

R

K

A

S

P

A

E

-

E

W

G

G

Y

P

L

M

G

G

V

M

V

V

V

V

G

E

G

G

K

K

P

S

Q

Y

F

W

E

F

T

R

R

L

V

P

D

A

K

K

I

R

H

H

T

T

L

V

R

N

S

S

V

E

F

F

D

D

V

I

R

K

Q

Q

L

I

K

S

T

S

L

L

P

P

K

Q

V

V

V

D

I

I

I