SitesBLAST

5k3sA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, bispyribac-sodium (see paper)
28% identity, 83% coverage: 3:483/577 of query aligns to 12:495/583 of 5k3sA

query
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5k3sA

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active site: Y33 (≠ I24), G35 (= G26), G36 (≠ V27), A37 (≠ H28), S38 (≠ N29), E59 (= E50), T82 (≠ V74), F121 (≠ L113), Q122 (≠ H114), E123 (= E115), K171 (≠ V163), M266 (≠ F253), V293 (≠ P282), V400 (vs. gap), G426 (≠ N414), M428 (= M416), D453 (= D441), N480 (≠ D468), H482 (≠ E470), L483 (= L471), M485 (≠ Q473), V486 (≠ I474), W489 (≠ A477)
binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: R292 (≠ I281), M485 (≠ Q473), W489 (≠ A477)
binding flavin-adenine dinucleotide: R161 (≠ Q153), G222 (= G210), G223 (≠ W211), G224 (= G212), T246 (= T234), L247 (= L235), M248 (≠ Q236), L264 (≠ F251), M266 (≠ F253), G286 (= G275), R288 (= R277), D290 (≠ G279), V293 (≠ P282), D310 (= D298), I311 (= I299), D329 (= D317), V330 (≠ A318), M405 (≠ F393), G423 (≠ T411)
binding magnesium ion: D453 (= D441), N480 (≠ D468), H482 (≠ E470)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (vs. gap), G401 (= G389), Q402 (≠ N390), H403 (= H391), G426 (≠ N414), M428 (= M416), D453 (= D441), G454 (= G442), S455 (≠ C443), N480 (≠ D468), H482 (≠ E470), L483 (= L471), G484 (≠ S472), M485 (≠ Q473), V486 (≠ I474)

Sites not aligning to the query:

active site: 558
binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: 569

5k2oA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, pyrithiobac (see paper)
28% identity, 83% coverage: 3:483/577 of query aligns to 12:495/585 of 5k2oA

query
sites
5k2oA

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active site: Y33 (≠ I24), G35 (= G26), G36 (≠ V27), A37 (≠ H28), S38 (≠ N29), E59 (= E50), T82 (≠ V74), F121 (≠ L113), Q122 (≠ H114), E123 (= E115), K171 (≠ V163), M266 (≠ F253), V293 (≠ P282), V400 (vs. gap), G426 (≠ N414), M428 (= M416), D453 (= D441), N480 (≠ D468), H482 (≠ E470), L483 (= L471), M485 (≠ Q473), V486 (≠ I474), W489 (≠ A477)
binding 2-chloranyl-6-(4,6-dimethoxypyrimidin-2-yl)sulfanyl-benzoic acid: M266 (≠ F253), R292 (≠ I281), W489 (≠ A477)
binding flavin-adenine dinucleotide: R161 (≠ Q153), G222 (= G210), G223 (≠ W211), G224 (= G212), T246 (= T234), L247 (= L235), M248 (≠ Q236), L264 (≠ F251), G286 (= G275), R288 (= R277), D290 (≠ G279), V293 (≠ P282), D310 (= D298), I311 (= I299), D329 (= D317), V330 (≠ A318), Q404 (≠ T392), M405 (≠ F393), G423 (≠ T411)
binding magnesium ion: D453 (= D441), N480 (≠ D468), H482 (≠ E470)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (vs. gap), G401 (= G389), Q402 (≠ N390), H403 (= H391), M428 (= M416), D453 (= D441), G454 (= G442), S455 (≠ C443), N480 (≠ D468), H482 (≠ E470), L483 (= L471), G484 (≠ S472), M485 (≠ Q473), V486 (≠ I474)

Sites not aligning to the query:

active site: 558
binding 2-chloranyl-6-(4,6-dimethoxypyrimidin-2-yl)sulfanyl-benzoic acid: 568

P17597 Acetolactate synthase, chloroplastic; AtALS; Acetohydroxy-acid synthase; Protein CHLORSULFURON RESISTANT 1; EC 2.2.1.6 from Arabidopsis thaliana (Mouse-ear cress) (see 8 papers)
28% identity, 83% coverage: 3:483/577 of query aligns to 97:580/670 of P17597

query
sites
P17597

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A

I

I

A

V

T

P

S

A

G

A

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G

I

A

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N

A

L

A

V

S

S

G

G

I

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G

A

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A

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x
S

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x
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x
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x
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K

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I

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x
K

N

D

L

I

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-

Q

-

L

-

A

-

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N

-

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A

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x
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x
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-

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D

I
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I

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N

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x
V

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K

E

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L

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E

A

N

R

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-

K

-

P

-

A

-

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-

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-

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-

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-

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L

A

K

R

L

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D

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-

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-

L

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-

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x
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H

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N

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R

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x
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x
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x
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M

G

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Y

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L

P

P

A

A

I

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P

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S

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Q

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E

I

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M

A

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A

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T

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A

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E

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N

L

L

G

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V

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Y

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L

V

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x
N

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x
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A122 (≠ H28) mutation to V: Reduced catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
M124 (≠ T30) mutation to E: Reduced catalytic activity. Resistant to imidazolinone herbicides and reduced sensitivity to sulfonylurea herbicides.; mutation to I: No effect on catalytic activity. Increased resistance to imidazolinone herbicides.
E144 (= E50) binding thiamine diphosphate
S186 (≠ G93) binding FAD
P197 (≠ R104) mutation to S: In csr1-1/GH50; resistant to sulfonylurea but not to imidazolinone herbicides.
R199 (≠ D106) mutation R->A,E: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
Q207 (≠ H114) binding thiamine diphosphate
K220 (= K127) binding (R)-imazaquin
R246 (≠ Q153) binding (R)-imazaquin; binding FAD
K256 (≠ V163) binding chlorimuron-ethyl
G308 (≠ W211) binding FAD
TL 331:332 (= TL 234:235) binding FAD
C340 (≠ A243) modified: Cysteine sulfinic acid (-SO2H)
LGMH 349:352 (≠ FGFS 251:254) binding FAD
GVRFD 371:375 (≠ GTRFG 275:279) binding FAD
DR 376:377 (≠ EI 280:281) binding chlorimuron-ethyl
DI 395:396 (= DI 298:299) binding FAD
DV 414:415 (≠ DA 317:318) binding FAD
QH 487:488 (≠ NH 390:391) binding thiamine diphosphate
GG 508:509 (≠ TD 411:412) binding FAD
GAM 511:513 (≠ NAM 414:416) binding thiamine diphosphate
D538 (= D441) binding Mg(2+)
DGS 538:540 (≠ DGC 441:443) binding thiamine diphosphate
N565 (≠ D468) binding Mg(2+)
NQHLGM 565:570 (≠ DGELSQ 468:473) binding thiamine diphosphate
H567 (≠ E470) binding Mg(2+)
W574 (≠ A477) binding chlorimuron-ethyl; mutation to L: Increased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.; mutation to S: Slightly decreased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.

Sites not aligning to the query:

653 binding chlorimuron-ethyl; S→A: No effect on catalytic activity or sensitivity to herbicides.; S→F: No effect on catalytic activity. Resistant to imidazolinone herbicides and also slightly sulfonylurea-resistant.; S→N: In csr1-2/GH90; no effect on catalytic activity. Resistant to imidazolinone but not to sulfonylurea herbicides.; S→T: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.

8et4A Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with amidosulfuron (see paper)
28% identity, 83% coverage: 3:483/577 of query aligns to 12:495/582 of 8et4A

query
sites
8et4A

K

R

T

K

A

G

A

A

W

D

L

I

A

L

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V

H

E

A

A

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L

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A

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-

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A

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I

A

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A

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A

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I

A

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A

L

A

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S

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G

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L

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L

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L

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A

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x
R

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P

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V

L

L

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E

D

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N

D

L

I

Q

Q

L

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-

Q

-

L

-

A

-

I

-

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-

N

-

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L

L

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L

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x
H

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-

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I

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S

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M

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M
x
Q

E

E

I

L

M

A

T

T

A

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A

V

R

E

G

N

L

L

G

P

V

V

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K

Y

V

F

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L

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N

G

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x
H

L
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L

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M

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K

binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (vs. gap), G401 (= G389), Q402 (≠ N390), H403 (= H391), G426 (≠ N414), M428 (= M416), G452 (= G440), D453 (= D441), G454 (= G442), S455 (≠ C443), M458 (= M446), N480 (≠ D468), H482 (≠ E470), L483 (= L471), G484 (≠ S472), M485 (≠ Q473), V486 (≠ I474)
binding flavin-adenine dinucleotide: R161 (≠ Q153), G222 (= G210), G223 (≠ W211), G224 (= G212), T246 (= T234), L247 (= L235), M248 (≠ Q236), L264 (≠ F251), M266 (≠ F253), H267 (≠ S254), G286 (= G275), V287 (≠ T276), R288 (= R277), D290 (≠ G279), R292 (≠ I281), V293 (≠ P282), D310 (= D298), I311 (= I299), D329 (= D317), V330 (≠ A318), M405 (≠ F393), G423 (≠ T411)
binding magnesium ion: F370 (≠ P367), D453 (= D441), M458 (= M446), Q461 (≠ M449), N480 (≠ D468), H482 (≠ E470)
binding N-{[(4,6-dimethoxypyrimidin-2-yl)carbamoyl]sulfamoyl}-N-methylmethanesulfonamide: M266 (≠ F253), R292 (≠ I281), M485 (≠ Q473), W489 (≠ A477)

Sites not aligning to the query:

5wj1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a triazolopyrimidine herbicide, penoxsulam (see paper)
28% identity, 83% coverage: 3:483/577 of query aligns to 12:495/582 of 5wj1A

query
sites
5wj1A

K

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K

A

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E

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-

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A

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I

A

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T

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A

P

G

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I

A

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N

A

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G

G

Q
x
R

P

P

G

G

P

P

V

V

L

L

V

V

E

D

I

V

P

P

V
x
K

N

D

L

I

Q

Q

L

Q

-

Q

-

L

-

A

-

I

-

P

-

N

-

W

-

E

-

Q

-

A

-

M

-

R

F

L

P

P

G

G

E

Y

V

M

G

S

E

R

L

M

P

P

R

K

W

-

T

-

P

-

P

P

A

E

S

D

P

S

D

H

P

L

E

E

A

Q

V

I

R

V

R

R

A

-

D

-

L

L

L

I

L

S

A

E

A

S

R

K

P

P

G

V

L

L

F

Y

V

V

G
|
G

W
x
G

G
|
G

A

C

R

L

G

N

A

S

Q

S

A

D

E

E

V

L

A

G

A

R

I

F

A

V

E

E

F

L

L

T

Q

G

A

I

P

P

V

V

S

A

T

S

T
|
T

L
|
L

Q
x
M

G

G

L

L

A

G

S

S

F

Y

P

P

A

C

D

D

H

D

P

E

L

L

H

S

A

L

G

H

-
x
M

F
x
L

G

G

F
x
M

S

H

G

G

S

T

A

V

V

Y

P

-

A

-

A

A

R

N

N

Y

A

A

F

V

E

E

G

H

C

S

D

D

G

L

M

L

L

L

A

A

V

F

G
|
G

T

V

R
|
R

F

F

G

D

E
x
D

I
x
R

P
x
V

T

T

G

G

S

K

F

L

S

E

A

A

E

F

V

A

P

S

-

R

A

A

G

K

L

I

V

V

H

H

V

I

D
|
D

I
|
I

C

D

A

S

D

A

V

E

I

I

G

G

A

K

N

N

Y

K

P

T

A

P

A

H

V

V

G

S

I

V

V

C

G

G

D
|
D

A
x
V

R

K

T

L

V

A

L

L

A

Q

A

G

L

M

L

N

E

K

E

V

L

L

R

E

A

N

R

R

G

-

K

-

P

-

A

-

P

-

D

-

T

-

G

-

L

-

A

A

D

E

R

E

L

L

A

K

R

L

D

D

R

F

R

G

A

V

W

W

R

R

E

N

E

E

W

L

Y

N

A

V

H

Q

D

K

S

Q

K

K

G

F

R

P

V

L

N

S

P

F

A

K

R

T

F

F

F

G

D

E

E

A

L

I

R

P

R

-

Q

-

M

-

P

P

D

Q

D

Y

A

A

V

I

T

K

V

V

L

L

D

D

D

E

-

L

-

T

-

D

-

G

-

K

-

A

-

I

-

S

-

T

-

G

-
x
V

G
|
G

N
x
Q

H
|
H

T

Q

F
x
M

L

W

T

A

A

A

E

Q

L

F

F

Y

P

N

I

Y

H

K

R

K

G

P

G

R

R

Q

V

W

L

L

T

S

P

S

T
x
G

D
x
G

F

L

N
x
G

A

A

M
|
M

G

G

Y

F

A

G

V

L

P

P

A

A

I

I

G

G

A

A

K

S

L

V

A

A

Q

N

P

P

G

D

K

A

E

I

V

V

F

V

A

D

I

I

V

D

G

G

D
|
D

G
|
G

C
x
S

F

F

L

I

M
|
M

T

N

C

V

M

Q

E

E

I

L

M

A

T

T

A

I

T

R

A

V

R

E

G

N

L

L

G

P

V

V

I

K

Y

V

F

L

V

L

F

L

S

N

D
x
N

G

Q

E
x
H

L
|
L

S
x
G

Q
x
M

I
x
V

A

M

Q

Q

A
x
W

Q

E

E

D

I

R

P

F

Y

Y

N

K

active site: Y33 (≠ I24), G35 (= G26), G36 (≠ V27), A37 (≠ H28), S38 (≠ N29), E59 (= E50), T82 (≠ V74), F121 (≠ L113), Q122 (≠ H114), E123 (= E115), K171 (≠ V163), M266 (≠ F253), V293 (≠ P282), V400 (vs. gap), G426 (≠ N414), M428 (= M416), D453 (= D441), N480 (≠ D468), H482 (≠ E470), L483 (= L471), M485 (≠ Q473), V486 (≠ I474), W489 (≠ A477)
binding flavin-adenine dinucleotide: R161 (≠ Q153), G222 (= G210), G223 (≠ W211), G224 (= G212), T246 (= T234), L247 (= L235), M248 (≠ Q236), M263 (vs. gap), L264 (≠ F251), G286 (= G275), R288 (= R277), V293 (≠ P282), D310 (= D298), I311 (= I299), D329 (= D317), V330 (≠ A318), M405 (≠ F393), G423 (≠ T411), G424 (≠ D412)
binding magnesium ion: D453 (= D441), N480 (≠ D468), H482 (≠ E470)
binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: M266 (≠ F253), D291 (≠ E280), R292 (≠ I281), M485 (≠ Q473), W489 (≠ A477)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (vs. gap), G401 (= G389), Q402 (≠ N390), H403 (= H391), M428 (= M416), D453 (= D441), G454 (= G442), S455 (≠ C443), M458 (= M446), N480 (≠ D468), H482 (≠ E470), L483 (= L471), G484 (≠ S472), M485 (≠ Q473), V486 (≠ I474)

Sites not aligning to the query:

5k6tA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, propoxycarbazone-sodium (see paper)
28% identity, 83% coverage: 3:483/577 of query aligns to 12:495/582 of 5k6tA

query
sites
5k6tA

K

R

T

K

A

G

A

A

W

D

L

I

A

L

V

V

H

E

A

A

M

L

E

E

Q

R

L

Q

G

G

I

V

R

E

F

T

T

V

F

F

G

A

I
x
Y

P

P

G
|
G

V
x
G

H
x
A

N
x
S

T

M

E

E

L

I

Y

H

D

Q

E

A

L

L

N

T

S

R

S

S

E

S

T

S

V

I

T

R

P

N

V

V

L

L

V

P

T

R

H

H

E
|
E

G

Q

G

G

A

V

F

F

M

A

A

A

D

E

A

G

V

Y

S

A

R

R

A

S

D

G

D

K

T

P

V

-

G

G

A

I

L

C

A

I

I

A

V
x
T

P

S

A

G

A

P

G

G

I

A

T

T

H

N

A

L

A

V

S

S

G

G

I

L

G

A

E

D

S

A

F

L

L

L

G

D

G

S

I

V

P

P

M

L

L

V

I

A

F

I

S

T

G

G

G

Q

I

V

R

P

T

R

D

R

T

M

G

I

R

G

R

T

F

D

Q

A

L
x
F

H
x
Q

E
|
E

I

T

D

P

Q

I

L

V

R

E

L

V

I

T

E

R

P

S

L

I

T

T

K

K

A

H

A

N

F

Y

R

L

V

V

E

M

R

D

H

V

E

E

D

D

V

I

V

P

P

R

T

I

I

I

H

E

E

E

A

A

Y

F

R

F

I

L

A

A

T

T

G

S

G

G

Q
x
R

P

P

G

G

P

P

V

V

L

L

V

V

E

D

I

V

P

P

V
x
K

N

D

L

I

Q

Q

L

Q

-

Q

-

L

-

A

-

I

-

P

-

N

-

W

-

E

-

Q

-

A

-

M

-

R

F

L

P

P

G

G

E

Y

V

M

G

S

E

R

L

M

P

P

R

K

W

-

T

-

P

-

P

P

A

E

S

D

P

S

D

H

P

L

E

E

A

Q

V

I

R

V

R

R

A

-

D

-

L

L

L

I

L

S

A

E

A

S

R

K

P

P

G

V

L

L

F

Y

V

V

G
|
G

W
x
G

G
|
G

A

C

R

L

G

N

A

S

Q

S

A

D

E

E

V

L

A

G

A

R

I

F

A

V

E

E

F

L

L

T

Q

G

A

I

P

P

V

V

S

A

T

S

T
|
T

L
|
L

Q
x
M

G

G

L

L

A

G

S

S

F

Y

P

P

A

C

D

D

H

D

P

E

L

L

H

S

A

L

G

H

-

M

F
x
L

G

G

F
x
M

S
x
H

G

G

S

T

A

V

V

Y

P

-

A

-

A

A

R

N

N

Y

A

A

F

V

E

E

G

H

C

S

D

D

G

L

M

L

L

L

A

A

V

F

G
|
G

T

V

R
|
R

F

F

G
x
D

E

D

I
x
R

P
x
V

T

T

G

G

S

K

F

L

S

E

A

A

E

F

V

A

P

S

-

R

A

A

G

K

L

I

V

V

H

H

V

I

D
|
D

I
|
I

C

D

A

S

D

A

V

E

I

I

G

G

A

K

N

N

Y

K

P

T

A

P

A

H

V

V

G

S

I

V

V

C

G

G

D
|
D

A
x
V

R

K

T

L

V

A

L

L

A

Q

A

G

L

M

L

N

E

K

E

V

L

L

R

E

A

N

R

R

G

-

K

-

P

-

A

-

P

-

D

-

T

-

G

-

L

-

A

A

D

E

R

E

L

L

A

K

R

L

D

D

R

F

R

G

A

V

W

W

R

R

E

N

E

E

W

L

Y

N

A

V

H

Q

D

K

S

Q

K

K

G

F

R

P

V

L

N

S

P

F

A

K

R

T

F

F

F

G

D

E

E

A

L

I

R

P

R

-

Q

-

M

-

P

P

D

Q

D

Y

A

A

V

I

T

K

V

V

L

L

D

D

D

E

-

L

-

T

-

D

-

G

-

K

-

A

-

I

-

S

-

T

-

G

-
x
V

G
|
G

N
x
Q

H
|
H

T
x
Q

F
x
M

L

W

T

A

A

A

E

Q

L

F

F

Y

P

N

I

Y

H

K

R

K

G

P

G

R

R

Q

V

W

L

L

T

S

P

S

T
x
G

D

G

F

L

N
x
G

A

A

M
|
M

G

G

Y

F

A

G

V

L

P

P

A

A

I

I

G

G

A

A

K

S

L

V

A

A

Q

N

P

P

G

D

K

A

E

I

V

V

F

V

A

D

I

I

V

D

G
|
G

D
|
D

G
|
G

C
x
S

F

F

L

I

M

M

T

N

C

V

M

Q

E

E

I

L

M

A

T

T

A

I

T

R

A

V

R

E

G

N

L

L

G

P

V

V

I

K

Y

V

F

L

V

L

F

L

S

N

D
x
N

G

Q

E
x
H

L
|
L

S
x
G

Q
x
M

I
x
V

A

M

Q

Q

A
x
W

Q

E

E

D

I

R

P

F

Y

Y

N

K

active site: Y33 (≠ I24), G35 (= G26), G36 (≠ V27), A37 (≠ H28), S38 (≠ N29), E59 (= E50), T82 (≠ V74), F121 (≠ L113), Q122 (≠ H114), E123 (= E115), K171 (≠ V163), M266 (≠ F253), V293 (≠ P282), V400 (vs. gap), G426 (≠ N414), M428 (= M416), D453 (= D441), N480 (≠ D468), H482 (≠ E470), L483 (= L471), M485 (≠ Q473), V486 (≠ I474), W489 (≠ A477)
binding methyl 2-[(4-methyl-5-oxidanylidene-3-propoxy-1,2,4-triazol-1-yl)carbonylsulfamoyl]benzoate: H267 (≠ S254), R292 (≠ I281), M485 (≠ Q473), W489 (≠ A477)
binding flavin-adenine dinucleotide: R161 (≠ Q153), G222 (= G210), G223 (≠ W211), G224 (= G212), T246 (= T234), L247 (= L235), M248 (≠ Q236), L264 (≠ F251), G286 (= G275), R288 (= R277), D290 (≠ G279), R292 (≠ I281), V293 (≠ P282), D310 (= D298), I311 (= I299), D329 (= D317), V330 (≠ A318), Q404 (≠ T392), M405 (≠ F393), G423 (≠ T411)
binding magnesium ion: D453 (= D441), N480 (≠ D468), H482 (≠ E470)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (vs. gap), G401 (= G389), Q402 (≠ N390), H403 (= H391), G426 (≠ N414), M428 (= M416), G452 (= G440), G454 (= G442), S455 (≠ C443), N480 (≠ D468), H482 (≠ E470), L483 (= L471), G484 (≠ S472)

Sites not aligning to the query:

5k6rA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, thiencarbazone-methyl (see paper)
28% identity, 83% coverage: 3:483/577 of query aligns to 12:495/582 of 5k6rA

query
sites
5k6rA

K

R

T

K

A

G

A

A

W

D

L

I

A

L

V

V

H

E

A

A

M

L

E

E

Q

R

L

Q

G

G

I

V

R

E

F

T

T

V

F

F

G

A

I
x
Y

P

P

G
|
G

V
x
G

H
x
A

N
x
S

T

M

E

E

L

I

Y

H

D

Q

E

A

L

L

N

T

S

R

S

S

E

S

T

S

V

I

T

R

P

N

V

V

L

L

V

P

T

R

H

H

E
|
E

G

Q

G

G

A

V

F

F

M

A

A

A

D

E

A

G

V

Y

S

A

R

R

A

S

D

G

D

K

T

P

V

-

G

G

A

I

L

C

A

I

I

A

V
x
T

P

S

A

G

A

P

G

G

I

A

T

T

H

N

A

L

A

V

S

S

G

G

I

L

G

A

E

D

S

A

F

L

L

L

G

D

G

S

I

V

P

P

M

L

L

V

I

A

F

I

S

T

G

G

G

Q

I

V

R

P

T

R

D

R

T

M

G

I

R

G

R

T

F

D

Q

A

L
x
F

H
x
Q

E
|
E

I

T

D

P

Q

I

L

V

R

E

L

V

I

T

E

R

P

S

L

I

T

T

K

K

A

H

A

N

F

Y

R

L

V

V

E

M

R

D

H

V

E

E

D

D

V

I

V

P

P

R

T

I

I

I

H

E

E

E

A

A

Y

F

R

F

I

L

A

A

T

T

G

S

G

G

Q
x
R

P

P

G

G

P

P

V

V

L

L

V

V

E

D

I

V

P

P

V
x
K

N

D

L

I

Q

Q

L

Q

-

Q

-

L

-

A

-

I

-

P

-

N

-

W

-

E

-

Q

-

A

-

M

-

R

F

L

P

P

G

G

E

Y

V

M

G

S

E

R

L

M

P

P

R

K

W

-

T

-

P

-

P

P

A

E

S

D

P

S

D

H

P

L

E

E

A

Q

V

I

R

V

R

R

A

-

D

-

L

L

L

I

L

S

A

E

A

S

R

K

P

P

G

V

L

L

F

Y

V

V

G
|
G

W
x
G

G
|
G

A

C

R

L

G

N

A

S

Q

S

A

D

E

E

V

L

A

G

A

R

I

F

A

V

E

E

F

L

L

T

Q

G

A

I

P

P

V

V

S

A

T

S

T
|
T

L
|
L

Q
x
M

G

G

L

L

A

G

S

S

F

Y

P

P

A

C

D

D

H

D

P

E

L

L

H

S

A

L

G

H

-

M

F
x
L

G

G

F
x
M

S

H

G

G

S

T

A

V

V

Y

P

-

A

-

A

A

R

N

N

Y

A

A

F

V

E

E

G

H

C

S

D

D

G

L

M

L

L

L

A

A

V

F

G
|
G

T

V

R
|
R

F

F

G

D

E

D

I
x
R

P
x
V

T

T

G

G

S

K

F

L

S

E

A

A

E

F

V

A

P

S

-

R

A

A

G

K

L

I

V

V

H

H

V

I

D
|
D

I
|
I

C

D

A

S

D

A

V

E

I

I

G

G

A

K

N

N

Y

K

P

T

A

P

A

H

V

V

G

S

I

V

V

C

G
|
G

D
|
D

A
x
V

R

K

T

L

V

A

L

L

A

Q

A

G

L

M

L

N

E

K

E

V

L

L

R

E

A

N

R

R

G

-

K

-

P

-

A

-

P

-

D

-

T

-

G

-

L

-

A

A

D

E

R

E

L

L

A

K

R

L

D

D

R

F

R

G

A

V

W

W

R

R

E

N

E

E

W

L

Y

N

A

V

H

Q

D

K

S

Q

K

K

G

F

R

P

V

L

N

S

P

F

A

K

R

T

F

F

F

G

D

E

E

A

L

I

R

P

R

-

Q

-

M

-

P

P

D

Q

D

Y

A

A

V

I

T

K

V

V

L

L

D

D

D

E

-

L

-

T

-

D

-

G

-

K

-

A

-

I

-

S

-

T

-

G

-
x
V

G
|
G

N
x
Q

H
|
H

T

Q

F
x
M

L

W

T

A

A

A

E

Q

L

F

F

Y

P

N

I

Y

H

K

R

K

G

P

G

R

R

Q

V

W

L

L

T

S

P

S

T
x
G

D

G

F

L

N
x
G

A

A

M
|
M

G

G

Y

F

A

G

V

L

P

P

A

A

I

I

G

G

A

A

K

S

L

V

A

A

Q

N

P

P

G

D

K

A

E

I

V

V

F

V

A

D

I

I

V

D

G

G

D
|
D

G
|
G

C
x
S

F

F

L

I

M
|
M

T

N

C

V

M

Q

E

E

I

L

M

A

T

T

A

I

T

R

A

V

R

E

G

N

L

L

G

P

V

V

I

K

Y

V

F

L

V

L

F

L

S

N

D
x
N

G

Q

E
x
H

L
|
L

S
x
G

Q
x
M

I
x
V

A

M

Q

Q

A
x
W

Q

E

E

D

I

R

P

F

Y

Y

N

K

active site: Y33 (≠ I24), G35 (= G26), G36 (≠ V27), A37 (≠ H28), S38 (≠ N29), E59 (= E50), T82 (≠ V74), F121 (≠ L113), Q122 (≠ H114), E123 (= E115), K171 (≠ V163), M266 (≠ F253), V293 (≠ P282), V400 (vs. gap), G426 (≠ N414), M428 (= M416), D453 (= D441), N480 (≠ D468), H482 (≠ E470), L483 (= L471), M485 (≠ Q473), V486 (≠ I474), W489 (≠ A477)
binding methyl 4-[(3-methoxy-4-methyl-5-oxidanylidene-1,2,4-triazol-1-yl)carbonylsulfamoyl]-5-methyl-thiophene-3-carboxylate: R292 (≠ I281), W489 (≠ A477)
binding flavin-adenine dinucleotide: R161 (≠ Q153), G222 (= G210), G223 (≠ W211), G224 (= G212), T246 (= T234), L247 (= L235), M248 (≠ Q236), L264 (≠ F251), M266 (≠ F253), G286 (= G275), R288 (= R277), R292 (≠ I281), V293 (≠ P282), D310 (= D298), I311 (= I299), G328 (= G316), D329 (= D317), V330 (≠ A318), M405 (≠ F393), G423 (≠ T411)
binding magnesium ion: D453 (= D441), N480 (≠ D468), H482 (≠ E470)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (vs. gap), G401 (= G389), Q402 (≠ N390), H403 (= H391), G426 (≠ N414), M428 (= M416), D453 (= D441), G454 (= G442), S455 (≠ C443), M458 (= M446), N480 (≠ D468), H482 (≠ E470), L483 (= L471), G484 (≠ S472), M485 (≠ Q473), V486 (≠ I474)

Sites not aligning to the query:

1z8nA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with an imidazolinone herbicide, imazaquin (see paper)
28% identity, 83% coverage: 3:483/577 of query aligns to 12:495/582 of 1z8nA

query
sites
1z8nA

K

R

T

K

A

G

A

A

W

D

L

I

A

L

V

V

H

E

A

A

M

L

E

E

Q

R

L

Q

G

G

I

V

R

E

F

T

T

V

F

F

G

A

I
x
Y

P

P

G
|
G

V
x
G

H
x
A

N
x
S

T

M

E

E

L

I

Y

H

D

Q

E

A

L

L

N

T

S

R

S

S

E

S

T

S

V

I

T

R

P

N

V

V

L

L

V

P

T

R

H

H

E
|
E

G

Q

G

G

A

V

F

F

M

A

A

A

D

E

A

G

V

Y

S

A

R

R

A

S

D

G

D

K

T

P

V

-

G

G

A

I

L

C

A

I

I

A

V
x
T

P

S

A

G

A

P

G

G

I

A

T

T

H

N

A

L

A

V

S

S

G

G

I

L

G

A

E

D

S

A

F

L

L

L

G

D

G

S

I

V

P

P

M

L

L

V

I

A

F

I

S

T

G

G

G

Q

I

V

R

P

T

R

D

R

T

M

G

I

R

G

R

T

F

D

Q

A

L
x
F

H
x
Q

E
|
E

I

T

D

P

Q

I

L

V

R

E

L

V

I

T

E

R

P

S

L

I

T

T

K
|
K

A

H

A

N

F

Y

R

L

V

V

E

M

R

D

H

V

E

E

D

D

V

I

V

P

P

R

T

I

I

I

H

E

E

E

A

A

Y

F

R

F

I

L

A

A

T

T

G

S

G

G

Q
x
R

P

P

G

G

P

P

V

V

L

L

V

V

E

D

I

V

P

P

V
x
K

N

D

L

I

Q

Q

L

Q

-

Q

-

L

-

A

-

I

-

P

-

N

-

W

-

E

-

Q

-

A

-

M

-

R

F

L

P

P

G

G

E
x
Y

V

M

G

S

E
x
R

L

M

P

P

R

K

W

-

T

-

P

-

P

P

A

E

S

D

P

S

D

H

P

L

E

E

A

Q

V

I

R

V

R

R

A

-

D

-

L

L

L

I

L

S

A

E

A

S

R

K

P

P

G

V

L

L

F

Y

V

V

G
|
G

W

G

G
|
G

A

C

R

L

G

N

A

S

Q

S

A

D

E

E

V

L

A

G

A

R

I

F

A

V

E

E

F

L

L

T

Q

G

A

I

P

P

V

V

S

A

T

S

T
|
T

L
|
L

Q
x
M

G

G

L

L

A

G

S

S

F

Y

P

P

A

C

D

D

H

D

P

E

L

L

H

S

A

L

G

H

-

M

F
x
L

G
|
G

F
x
M

S
x
H

G

G

S

T

A

V

V

Y

P

-

A

-

A

A

R

N

N

Y

A

A

F

V

E

E

G

H

C

S

D

D

G

L

M

L

L

L

A

A

V

F

G
|
G

T
x
V

R
|
R

F

F

G
x
D

E
x
D

I
x
R

P
x
V

T

T

G

G

S

K

F

L

S

E

A

A

E

F

V

A

P

S

-

R

A

A

G

K

L

I

V

V

H

H

V

I

D
|
D

I
|
I

C
x
D

A

S

D

A

V

E

I

I

G

G

A

K

N

N

Y

K

P

T

A

P

A

H

V

V

G

S

I

V

V

C

G

G

D
|
D

A
x
V

R

K

T

L

V

A

L

L

A

Q

A

G

L

M

L

N

E

K

E

V

L

L

R

E

A

N

R

R

G

-

K

-

P

-

A

-

P

-

D

-

T

-

G

-

L

-

A

A

D

E

R

E

L

L

A

K

R

L

D

D

R

F

R

G

A

V

W

W

R

R

E

N

E

E

W

L

Y

N

A

V

H

Q

D

K

S

Q

K

K

G

F

R

P

V

L

N

S

P

F

A

K

R

T

F

F

F

G

D

E

E

A

L

I

R

P

R

-

Q

-

M

-

P

P

D

Q

D

Y

A

A

V

I

T

K

V

V

L

L

D

D

D

E

-

L

-

T

-

D

-

G

-

K

-

A

-

I

-

S

-

T

-

G

-
x
V

G
|
G

N
x
Q

H
|
H

T

Q

F
x
M

L

W

T

A

A

A

E

Q

L

F

F

Y

P

N

I

Y

H

K

R

K

G

P

G

R

R

Q

V

W

L

L

T

S

P

S

T
x
G

D
x
G

F

L

N
x
G

A

A

M
|
M

G

G

Y

F

A

G

V

L

P

P

A

A

I

I

G

G

A

A

K

S

L

V

A

A

Q

N

P

P

G

D

K

A

E

I

V

V

F

V

A

D

I

I

V

D

G
|
G

D
|
D

G
|
G

C
x
S

F

F

L

I

M

M

T

N

C

V

M

Q

E

E

I

L

M

A

T

T

A

I

T

R

A

V

R

E

G

N

L

L

G

P

V

V

I

K

Y

V

F

L

V

L

F

L

S

N

D
x
N

G

Q

E
x
H

L
|
L

S
x
G

Q
x
M

I
x
V

A

M

Q

Q

A
x
W

Q

E

E

D

I

R

P

F

Y

Y

N

K

active site: Y33 (≠ I24), G35 (= G26), G36 (≠ V27), A37 (≠ H28), S38 (≠ N29), E59 (= E50), T82 (≠ V74), F121 (≠ L113), Q122 (≠ H114), E123 (= E115), K171 (≠ V163), M266 (≠ F253), V293 (≠ P282), V400 (vs. gap), G426 (≠ N414), M428 (= M416), D453 (= D441), N480 (≠ D468), H482 (≠ E470), L483 (= L471), M485 (≠ Q473), V486 (≠ I474), W489 (≠ A477)
binding 2-(4-isopropyl-4-methyl-5-oxo-4,5-dihydro-1h-imidazol-2-yl)quinoline-3-carboxylic acid: K135 (= K127), R161 (≠ Q153), Y191 (≠ E171), R194 (≠ E174), D291 (≠ E280), R292 (≠ I281), D312 (≠ C300), W489 (≠ A477)
binding flavin-adenine dinucleotide: R161 (≠ Q153), G222 (= G210), G224 (= G212), T246 (= T234), L247 (= L235), M248 (≠ Q236), L264 (≠ F251), G265 (= G252), M266 (≠ F253), H267 (≠ S254), G286 (= G275), V287 (≠ T276), R288 (= R277), D290 (≠ G279), R292 (≠ I281), V293 (≠ P282), D310 (= D298), I311 (= I299), D329 (= D317), V330 (≠ A318), M405 (≠ F393), G423 (≠ T411), G424 (≠ D412)
binding magnesium ion: D453 (= D441), N480 (≠ D468)
binding thiamine diphosphate: V400 (vs. gap), G401 (= G389), Q402 (≠ N390), H403 (= H391), G426 (≠ N414), M428 (= M416), G452 (= G440), G454 (= G442), S455 (≠ C443), N480 (≠ D468), H482 (≠ E470), L483 (= L471), G484 (≠ S472), M485 (≠ Q473), V486 (≠ I474)

Sites not aligning to the query:

1yi1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, tribenuron methyl (see paper)
28% identity, 83% coverage: 3:483/577 of query aligns to 12:495/582 of 1yi1A

query
sites
1yi1A

K

R

T

K

A

G

A

A

W

D

L

I

A

L

V

V

H

E

A

A

M

L

E

E

Q

R

L

Q

G

G

I

V

R

E

F

T

T

V

F

F

G

A

I
x
Y

P

P

G
|
G

V
x
G

H
x
A

N
x
S

T

M

E

E

L

I

Y

H

D

Q

E

A

L

L

N

T

S

R

S

S

E

S

T

S

V

I

T

R

P

N

V

V

L

L

V

P

T

R

H

H

E
|
E

G

Q

G

G

A

V

F

F

M

A

A

A

D

E

A

G

V

Y

S

A

R

R

A

S

D

G

D

K

T

P

V

-

G

G

A

I

L

C

A

I

I

A

V
x
T

P

S

A

G

A

P

G

G

I

A

T

T

H

N

A

L

A

V

S

S

G

G

I

L

G

A

E

D

S

A

F

L

L

L

G

D

G

S

I

V

P

P

M

L

L

V

I

A

F

I

S

T

G

G

G

Q

I

V

R

P

T

R

D

R

T

M

G

I

R

G

R

T

F

D

Q

A

L
x
F

H
x
Q

E
|
E

I

T

D

P

Q

I

L

V

R

E

L

V

I

T

E

R

P

S

L

I

T

T

K

K

A

H

A

N

F

Y

R

L

V

V

E

M

R

D

H

V

E

E

D

D

V

I

V

P

P

R

T

I

I

I

H

E

E

E

A

A

Y

F

R

F

I

L

A

A

T

T

G

S

G

G

Q
x
R

P

P

G

G

P

P

V

V

L

L

V

V

E

D

I

V

P

P

V
x
K

N

D

L

I

Q

Q

L

Q

-

Q

-

L

-

A

-

I

-

P

-

N

-

W

-

E

-

Q

-

A

-

M

-

R

F

L

P

P

G

G

E

Y

V

M

G

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E

R

L

M

P

P

R

K

W

-

T

-

P

-

P

P

A

E

S

D

P

S

D

H

P

L

E

E

A

Q

V

I

R

V

R

R

A

-

D

-

L

L

L

I

L

S

A

E

A

S

R

K

P

P

G

V

L

L

F

Y

V

V

G

G

W
x
G

G
|
G

A

C

R

L

G

N

A

S

Q

S

A

D

E

E

V

L

A

G

A

R

I

F

A

V

E

E

F

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L

T

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G

A

I

P

P

V

V

S

A

T

S

T
|
T

L
|
L

Q
x
M

G

G

L

L

A

G

S

S

F

Y

P

P

A

C

D

D

H

D

P

E

L

L

H

S

A

L

G

H

-
x
M

F
x
L

G
|
G

F
x
M

S
x
H

G

G

S

T

A

V

V

Y

P

-

A

-

A

A

R

N

N

Y

A

A

F

V

E

E

G

H

C

S

D

D

G

L

M

L

L

L

A

A

V

F

G
|
G

T
x
V

R
|
R

F

F

G
x
D

E
x
D

I
x
R

P
x
V

T

T

G

G

S

K

F

L

S

E

A

A

E

F

V

A

P

S

-

R

A

A

G

K

L

I

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V

H

H

V

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D
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D

I
|
I

C

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A

S

D

A

V

E

I

I

G

G

A

K

N

N

Y

K

P

T

A

P

A

H

V

V

G

S

I

V

V

C

G

G

D
|
D

A
x
V

R

K

T

L

V

A

L

L

A

Q

A

G

L

M

L

N

E

K

E

V

L

L

R

E

A

N

R

R

G

-

K

-

P

-

A

-

P

-

D

-

T

-

G

-

L

-

A

A

D

E

R

E

L

L

A

K

R

L

D

D

R

F

R

G

A

V

W

W

R

R

E

N

E

E

W

L

Y

N

A

V

H

Q

D

K

S

Q

K

K

G

F

R

P

V

L

N

S

P

F

A

K

R

T

F

F

F

G

D

E

E

A

L

I

R

P

R

-

Q

-

M

-

P

P

D

Q

D

Y

A

A

V

I

T

K

V

V

L

L

D

D

D

E

-

L

-

T

-

D

-

G

-

K

-

A

-

I

-

S

-

T

-

G

-
x
V

G

G

N

Q

H

H

T

Q

F
x
M

L

W

T

A

A

A

E

Q

L

F

F

Y

P

N

I

Y

H

K

R

K

G

P

G

R

R

Q

V

W

L

L

T

S

P

S

T
x
G

D
x
G

F

L

N
x
G

A

A

M
|
M

G

G

Y

F

A

G

V

L

P

P

A

A

I

I

G

G

A

A

K

S

L

V

A

A

Q

N

P

P

G

D

K

A

E

I

V

V

F

V

A

D

I

I

V

D

G

G

D
|
D

G

G

C

S

F

F

L

I

M

M

T

N

C

V

M

Q

E

E

I

L

M

A

T

T

A

I

T

R

A

V

R

E

G

N

L

L

G

P

V

V

I

K

Y

V

F

L

V

L

F

L

S

N

D
x
N

G

Q

E
x
H

L
|
L

S

G

Q
x
M

I
x
V

A

M

Q

Q

A
x
W

Q

E

E

D

I

R

P

F

Y

Y

N

K

active site: Y33 (≠ I24), G35 (= G26), G36 (≠ V27), A37 (≠ H28), S38 (≠ N29), E59 (= E50), T82 (≠ V74), F121 (≠ L113), Q122 (≠ H114), E123 (= E115), K171 (≠ V163), M266 (≠ F253), V293 (≠ P282), V400 (vs. gap), G426 (≠ N414), M428 (= M416), D453 (= D441), N480 (≠ D468), H482 (≠ E470), L483 (= L471), M485 (≠ Q473), V486 (≠ I474), W489 (≠ A477)
binding methyl 2-[4-methoxy-6-methyl-1,3,5-trazin-2-yl(methyl)carbamoylsulfamoyl]benzoate: D291 (≠ E280), R292 (≠ I281), W489 (≠ A477)
binding flavin-adenine dinucleotide: R161 (≠ Q153), G223 (≠ W211), G224 (= G212), T246 (= T234), L247 (= L235), M248 (≠ Q236), M263 (vs. gap), L264 (≠ F251), G265 (= G252), M266 (≠ F253), H267 (≠ S254), G286 (= G275), V287 (≠ T276), R288 (= R277), D290 (≠ G279), V293 (≠ P282), D310 (= D298), I311 (= I299), D329 (= D317), V330 (≠ A318), M405 (≠ F393), G423 (≠ T411), G424 (≠ D412)
binding magnesium ion: D453 (= D441), N480 (≠ D468), H482 (≠ E470)

Sites not aligning to the query:

1yi0A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, sulfometuron methyl (see paper)
28% identity, 83% coverage: 3:483/577 of query aligns to 12:495/582 of 1yi0A

query
sites
1yi0A

K

R

T

K

A

G

A

A

W

D

L

I

A

L

V

V

H

E

A

A

M

L

E

E

Q

R

L

Q

G

G

I

V

R

E

F

T

T

V

F

F

G

A

I
x
Y

P

P

G
|
G

V
x
G

H
x
A

N
x
S

T

M

E

E

L

I

Y

H

D

Q

E

A

L

L

N

T

S

R

S

S

E

S

T

S

V

I

T

R

P

N

V

V

L

L

V

P

T

R

H

H

E
|
E

G

Q

G

G

A

V

F

F

M

A

A

A

D

E

A

G

V

Y

S

A

R

R

A

S

D

G

D

K

T

P

V

-

G

G

A

I

L

C

A

I

I

A

V
x
T

P

S

A

G

A

P

G

G

I

A

T

T

H

N

A

L

A

V

S

S

G

G

I

L

G

A

E

D

S

A

F

L

L

L

G

D

G

S

I

V

P

P

M

L

L

V

I

A

F

I

S

T

G

G

G

Q

I

V

R

P

T

R

D

R

T

M

G

I

R

G

R

T

F

D

Q

A

L
x
F

H
x
Q

E
|
E

I

T

D

P

Q

I

L

V

R

E

L

V

I

T

E

R

P

S

L

I

T

T

K

K

A

H

A

N

F

Y

R

L

V

V

E

M

R

D

H

V

E

E

D

D

V

I

V

P

P

R

T

I

I

I

H

E

E

E

A

A

Y

F

R

F

I

L

A

A

T

T

G

S

G

G

Q
x
R

P

P

G

G

P

P

V

V

L

L

V

V

E

D

I

V

P

P

V
x
K

N

D

L

I

Q

Q

L

Q

-

Q

-

L

-

A

-

I

-

P

-

N

-

W

-

E

-

Q

-

A

-

M

-

R

F

L

P

P

G

G

E

Y

V

M

G

S

E

R

L

M

P

P

R

K

W

-

T

-

P

-

P

P

A

E

S

D

P

S

D

H

P

L

E

E

A

Q

V

I

R

V

R

R

A

-

D

-

L

L

L

I

L

S

A

E

A

S

R

K

P

P

G

V

L

L

F

Y

V

V

G
|
G

W
x
G

G
|
G

A

C

R

L

G

N

A

S

Q

S

A

D

E

E

V

L

A

G

A

R

I

F

A

V

E

E

F

L

L

T

Q

G

A

I

P

P

V

V

S

A

T

S

T
|
T

L
|
L

Q
x
M

G

G

L

L

A

G

S

S

F

Y

P

P

A

C

D

D

H

D

P

E

L

L

H

S

A

L

G

H

-

M

F
x
L

G
|
G

F
x
M

S
x
H

G

G

S

T

A

V

V

Y

P

-

A

-

A

A

R

N

N

Y

A

A

F

V

E

E

G

H

C

S

D

D

G

L

M

L

L

L

A

A

V

F

G
|
G

T
x
V

R
|
R

F

F

G
x
D

E
x
D

I
x
R

P
x
V

T

T

G

G

S

K

F

L

S

E

A

A

E

F

V

A

P

S

-

R

A

A

G

K

L

I

V

V

H

H

V

I

D
|
D

I
|
I

C

D

A

S

D

A

V

E

I

I

G

G

A

K

N

N

Y

K

P

T

A

P

A

H

V

V

G

S

I

V

V

C

G
|
G

D
|
D

A
x
V

R

K

T

L

V

A

L

L

A

Q

A

G

L

M

L

N

E

K

E

V

L

L

R

E

A

N

R

R

G

-

K

-

P

-

A

-

P

-

D

-

T

-

G

-

L

-

A

A

D

E

R

E

L

L

A

K

R

L

D

D

R

F

R

G

A

V

W

W

R

R

E

N

E

E

W

L

Y

N

A

V

H

Q

D

K

S

Q

K

K

G

F

R

P

V

L

N

S

P

F

A

K

R

T

F

F

F

G

D

E

E

A

L

I

R

P

R

-

Q

-

M

-

P

P

D

Q

D

Y

A

A

V

I

T

K

V

V

L

L

D

D

D

E

-

L

-

T

-

D

-

G

-

K

-

A

-

I

-

S

-

T

-

G

-
x
V

G

G

N

Q

H

H

T

Q

F
x
M

L

W

T

A

A

A

E

Q

L

F

F

Y

P

N

I

Y

H

K

R

K

G

P

G

R

R

Q

V

W

L

L

T

S

P

S

T
x
G

D
x
G

F

L

N
x
G

A

A

M
|
M

G

G

Y

F

A

G

V

L

P

P

A

A

I

I

G

G

A

A

K

S

L

V

A

A

Q

N

P

P

G

D

K

A

E

I

V

V

F

V

A

D

I

I

V

D

G

G

D
|
D

G

G

C

S

F

F

L

I

M

M

T

N

C

V

M

Q

E

E

I

L

M

A

T

T

A

I

T

R

A

V

R

E

G

N

L

L

G

P

V

V

I

K

Y

V

F

L

V

L

F

L

S

N

D
x
N

G

Q

E
x
H

L
|
L

S

G

Q
x
M

I
x
V

A

M

Q

Q

A
x
W

Q

E

E

D

I

R

P

F

Y

Y

N

K

active site: Y33 (≠ I24), G35 (= G26), G36 (≠ V27), A37 (≠ H28), S38 (≠ N29), E59 (= E50), T82 (≠ V74), F121 (≠ L113), Q122 (≠ H114), E123 (= E115), K171 (≠ V163), M266 (≠ F253), V293 (≠ P282), V400 (vs. gap), G426 (≠ N414), M428 (= M416), D453 (= D441), N480 (≠ D468), H482 (≠ E470), L483 (= L471), M485 (≠ Q473), V486 (≠ I474), W489 (≠ A477)
binding methyl 2-[({[(4,6-dimethylpyrimidin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D291 (≠ E280), R292 (≠ I281), W489 (≠ A477)
binding flavin-adenine dinucleotide: R161 (≠ Q153), G222 (= G210), G223 (≠ W211), G224 (= G212), T246 (= T234), L247 (= L235), M248 (≠ Q236), L264 (≠ F251), G265 (= G252), M266 (≠ F253), H267 (≠ S254), G286 (= G275), V287 (≠ T276), R288 (= R277), D290 (≠ G279), R292 (≠ I281), V293 (≠ P282), D310 (= D298), I311 (= I299), G328 (= G316), D329 (= D317), V330 (≠ A318), M405 (≠ F393), G423 (≠ T411), G424 (≠ D412)
binding magnesium ion: D453 (= D441), N480 (≠ D468), H482 (≠ E470)

Sites not aligning to the query:

1yhzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorsulfuron (see paper)
28% identity, 83% coverage: 3:483/577 of query aligns to 12:495/582 of 1yhzA

query
sites
1yhzA

K

R

T

K

A

G

A

A

W

D

L

I

A

L

V

V

H

E

A

A

M

L

E

E

Q

R

L

Q

G

G

I

V

R

E

F

T

T

V

F

F

G

A

I
x
Y

P

P

G
|
G

V
x
G

H
x
A

N
x
S

T

M

E

E

L

I

Y

H

D

Q

E

A

L

L

N

T

S

R

S

S

E

S

T

S

V

I

T

R

P

N

V

V

L

L

V

P

T

R

H

H

E
|
E

G

Q

G

G

A

V

F

F

M

A

A

A

D

E

A

G

V

Y

S

A

R

R

A

S

D

G

D

K

T

P

V

-

G

G

A

I

L

C

A

I

I

A

V
x
T

P

S

A

G

A

P

G

G

I

A

T

T

H

N

A

L

A

V

S

S

G

G

I

L

G

A

E

D

S

A

F

L

L

L

G

D

G

S

I

V

P

P

M

L

L

V

I

A

F

I

S

T

G

G

G

Q

I

V

R

P

T

R

D

R

T

M

G

I

R

G

R

T

F

D

Q

A

L
x
F

H
x
Q

E
|
E

I

T

D

P

Q

I

L

V

R

E

L

V

I

T

E

R

P

S

L

I

T

T

K

K

A

H

A

N

F

Y

R

L

V

V

E

M

R

D

H

V

E

E

D

D

V

I

V

P

P

R

T

I

I

I

H

E

E

E

A

A

Y

F

R

F

I

L

A

A

T

T

G

S

G

G

Q
x
R

P

P

G

G

P

P

V

V

L

L

V

V

E

D

I

V

P

P

V
x
K

N

D

L

I

Q

Q

L

Q

-

Q

-

L

-

A

-

I

-

P

-

N

-

W

-

E

-

Q

-

A

-

M

-

R

F

L

P

P

G

G

E

Y

V

M

G

S

E

R

L

M

P

P

R

K

W

-

T

-

P

-

P

P

A

E

S

D

P

S

D

H

P

L

E

E

A

Q

V

I

R

V

R

R

A

-

D

-

L

L

L

I

L

S

A

E

A

S

R

K

P

P

G

V

L

L

F

Y

V

V

G

G

W
x
G

G
|
G

A

C

R

L

G

N

A

S

Q

S

A

D

E

E

V

L

A

G

A

R

I

F

A

V

E

E

F

L

L

T

Q

G

A

I

P

P

V

V

S

A

T

S

T
|
T

L
|
L

Q
x
M

G

G

L

L

A

G

S

S

F

Y

P

P

A

C

D

D

H

D

P

E

L

L

H

S

A

L

G

H

-

M

F
x
L

G

G

F
x
M

S
x
H

G

G

S

T

A

V

V

Y

P

-

A

-

A

A

R

N

N

Y

A

A

F

V

E

E

G

H

C

S

D

D

G

L

M

L

L

L

A

A

V

F

G
|
G

T
x
V

R
|
R

F

F

G
x
D

E
x
D

I
x
R

P
x
V

T

T

G

G

S

K

F

L

S

E

A

A

E

F

V

A

P

S

-

R

A

A

G

K

L

I

V

V

H

H

V

I

D
|
D

I
|
I

C

D

A

S

D

A

V

E

I

I

G

G

A

K

N

N

Y

K

P

T

A

P

A

H

V

V

G

S

I

V

V

C

G

G

D
|
D

A
x
V

R

K

T

L

V

A

L

L

A

Q

A

G

L

M

L

N

E

K

E

V

L

L

R

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A

N

R

R

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K

-

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-

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L

A

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L

D

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A

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W

R

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E

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E

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L

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A

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Q

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K

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L

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P

F

A

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F

F

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D

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E

A

L

I

R

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R

-

Q

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-

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P

D

Q

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Y

A

A

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I

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V

L

L

D

D

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E

-

L

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T

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-

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-

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-

A

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-

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-
x
V

G

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H

H

T
x
Q

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x
M

L

W

T

A

A

A

E

Q

L

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F

Y

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N

I

Y

H

K

R

K

G

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G

R

R

Q

V

W

L

L

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S

P

S

T
x
G

D
x
G

F

L

N
x
G

A

A

M
|
M

G

G

Y

F

A

G

V

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P

P

A

A

I

I

G

G

A

A

K

S

L

V

A

A

Q

N

P

P

G

D

K

A

E

I

V

V

F

V

A

D

I

I

V

D

G

G

D
|
D

G

G

C

S

F

F

L

I

M

M

T

N

C

V

M

Q

E

E

I

L

M

A

T

T

A

I

T

R

A

V

R

E

G

N

L

L

G

P

V

V

I

K

Y

V

F

L

V

L

F

L

S

N

D
x
N

G

Q

E
x
H

L
|
L

S

G

Q
x
M

I
x
V

A

M

Q

Q

A
x
W

Q

E

E

D

I

R

P

F

Y

Y

N

K

active site: Y33 (≠ I24), G35 (= G26), G36 (≠ V27), A37 (≠ H28), S38 (≠ N29), E59 (= E50), T82 (≠ V74), F121 (≠ L113), Q122 (≠ H114), E123 (= E115), K171 (≠ V163), M266 (≠ F253), V293 (≠ P282), V400 (vs. gap), G426 (≠ N414), M428 (= M416), D453 (= D441), N480 (≠ D468), H482 (≠ E470), L483 (= L471), M485 (≠ Q473), V486 (≠ I474), W489 (≠ A477)
binding 1-(2-chlorophenylsulfonyl)-3-(4-methoxy-6-methyl-l,3,5-triazin-2-yl)urea: D291 (≠ E280), R292 (≠ I281), M485 (≠ Q473), W489 (≠ A477)
binding flavin-adenine dinucleotide: R161 (≠ Q153), G223 (≠ W211), G224 (= G212), T246 (= T234), L247 (= L235), M248 (≠ Q236), L264 (≠ F251), M266 (≠ F253), H267 (≠ S254), G286 (= G275), V287 (≠ T276), R288 (= R277), D290 (≠ G279), V293 (≠ P282), D310 (= D298), I311 (= I299), D329 (= D317), V330 (≠ A318), Q404 (≠ T392), M405 (≠ F393), G423 (≠ T411), G424 (≠ D412)
binding magnesium ion: D453 (= D441), N480 (≠ D468), H482 (≠ E470)

Sites not aligning to the query:

1yhyA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, metsulfuron methyl (see paper)
28% identity, 83% coverage: 3:483/577 of query aligns to 12:495/582 of 1yhyA

query
sites
1yhyA

K

R

T

K

A

G

A

A

W

D

L

I

A

L

V

V

H

E

A

A

M

L

E

E

Q

R

L

Q

G

G

I

V

R

E

F

T

T

V

F

F

G

A

I
x
Y

P

P

G
|
G

V
x
G

H
x
A

N
x
S

T

M

E

E

L

I

Y

H

D

Q

E

A

L

L

N

T

S

R

S

S

E

S

T

S

V

I

T

R

P

N

V

V

L

L

V

P

T

R

H

H

E
|
E

G

Q

G

G

A

V

F

F

M

A

A

A

D

E

A

G

V

Y

S

A

R

R

A

S

D

G

D

K

T

P

V

-

G

G

A

I

L

C

A

I

I

A

V
x
T

P

S

A

G

A

P

G

G

I

A

T

T

H

N

A

L

A

V

S

S

G

G

I

L

G

A

E

D

S

A

F

L

L

L

G

D

G

S

I

V

P

P

M

L

L

V

I

A

F

I

S

T

G

G

G

Q

I

V

R

P

T

R

D

R

T

M

G

I

R

G

R

T

F

D

Q

A

L
x
F

H
x
Q

E
|
E

I

T

D

P

Q

I

L

V

R

E

L

V

I

T

E

R

P

S

L

I

T

T

K

K

A

H

A

N

F

Y

R

L

V

V

E

M

R

D

H

V

E

E

D

D

V

I

V

P

P

R

T

I

I

I

H

E

E

E

A

A

Y

F

R

F

I

L

A

A

T

T

G

S

G

G

Q
x
R

P

P

G

G

P

P

V

V

L

L

V

V

E

D

I

V

P

P

V
x
K

N

D

L

I

Q

Q

L

Q

-

Q

-

L

-

A

-

I

-

P

-

N

-

W

-

E

-

Q

-

A

-

M

-

R

F

L

P

P

G

G

E

Y

V

M

G

S

E

R

L

M

P

P

R

K

W

-

T

-

P

-

P

P

A

E

S

D

P

S

D

H

P

L

E

E

A

Q

V

I

R

V

R

R

A

-

D

-

L

L

L

I

L

S

A

E

A

S

R

K

P

P

G

V

L

L

F

Y

V

V

G
|
G

W
x
G

G
|
G

A

C

R

L

G

N

A

S

Q

S

A

D

E

E

V

L

A

G

A

R

I

F

A

V

E

E

F

L

L

T

Q

G

A

I

P

P

V

V

S

A

T

S

T
|
T

L
|
L

Q
x
M

G

G

L

L

A

G

S

S

F

Y

P

P

A

C

D

D

H

D

P

E

L

L

H

S

A

L

G

H

-

M

F
x
L

G
|
G

F
x
M

S
x
H

G

G

S

T

A

V

V

Y

P

-

A

-

A

A

R

N

N

Y

A

A

F

V

E

E

G

H

C

S

D

D

G

L

M

L

L

L

A

A

V

F

G
|
G

T
x
V

R
|
R

F

F

G
x
D

E
x
D

I
x
R

P
x
V

T

T

G

G

S

K

F

L

S

E

A

A

E

F

V

A

P

S

-

R

A

A

G

K

L

I

V

V

H

H

V

I

D
|
D

I
|
I

C

D

A

S

D

A

V

E

I

I

G

G

A

K

N

N

Y

K

P

T

A

P

A

H

V

V

G

S

I

V

V

C

G

G

D
|
D

A
x
V

R

K

T

L

V

A

L

L

A

Q

A

G

L

M

L

N

E

K

E

V

L

L

R

E

A

N

R

R

G

-

K

-

P

-

A

-

P

-

D

-

T

-

G

-

L

-

A

A

D

E

R

E

L

L

A

K

R

L

D

D

R

F

R

G

A

V

W

W

R

R

E

N

E

E

W

L

Y

N

A

V

H

Q

D

K

S

Q

K

K

G

F

R

P

V

L

N

S

P

F

A

K

R

T

F

F

F

G

D

E

E

A

L

I

R

P

R

-

Q

-

M

-

P

P

D

Q

D

Y

A

A

V

I

T

K

V

V

L

L

D

D

D

E

-

L

-

T

-

D

-

G

-

K

-

A

-

I

-

S

-

T

-

G

-
x
V

G

G

N

Q

H

H

T
x
Q

F
x
M

L

W

T

A

A

A

E

Q

L

F

F

Y

P

N

I

Y

H

K

R

K

G

P

G

R

R

Q

V

W

L

L

T

S

P

S

T
x
G

D
x
G

F

L

N
x
G

A

A

M
|
M

G

G

Y

F

A

G

V

L

P

P

A

A

I

I

G

G

A

A

K

S

L

V

A

A

Q

N

P

P

G

D

K

A

E

I

V

V

F

V

A

D

I

I

V

D

G

G

D
|
D

G

G

C

S

F

F

L

I

M

M

T

N

C

V

M

Q

E

E

I

L

M

A

T

T

A

I

T

R

A

V

R

E

G

N

L

L

G

P

V

V

I

K

Y

V

F

L

V

L

F

L

S

N

D
x
N

G

Q

E
x
H

L
|
L

S

G

Q
x
M

I
x
V

A

M

Q

Q

A
x
W

Q

E

E

D

I

R

P

F

Y

Y

N

K

active site: Y33 (≠ I24), G35 (= G26), G36 (≠ V27), A37 (≠ H28), S38 (≠ N29), E59 (= E50), T82 (≠ V74), F121 (≠ L113), Q122 (≠ H114), E123 (= E115), K171 (≠ V163), M266 (≠ F253), V293 (≠ P282), V400 (vs. gap), G426 (≠ N414), M428 (= M416), D453 (= D441), N480 (≠ D468), H482 (≠ E470), L483 (= L471), M485 (≠ Q473), V486 (≠ I474), W489 (≠ A477)
binding methyl 2-[({[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D291 (≠ E280), R292 (≠ I281), V486 (≠ I474), W489 (≠ A477)
binding flavin-adenine dinucleotide: R161 (≠ Q153), G222 (= G210), G223 (≠ W211), G224 (= G212), T246 (= T234), L247 (= L235), M248 (≠ Q236), L264 (≠ F251), G265 (= G252), M266 (≠ F253), H267 (≠ S254), G286 (= G275), V287 (≠ T276), R288 (= R277), D290 (≠ G279), V293 (≠ P282), D310 (= D298), I311 (= I299), D329 (= D317), V330 (≠ A318), Q404 (≠ T392), M405 (≠ F393), G423 (≠ T411), G424 (≠ D412)
binding magnesium ion: D453 (= D441), N480 (≠ D468), H482 (≠ E470)

Sites not aligning to the query:

1ybhA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide chlorimuron ethyl (see paper)
28% identity, 83% coverage: 3:483/577 of query aligns to 12:495/582 of 1ybhA

query
sites
1ybhA

K

R

T

K

A

G

A

A

W

D

L

I

A

L

V

V

H

E

A

A

M

L

E

E

Q

R

L

Q

G

G

I

V

R

E

F

T

T

V

F

F

G

A

I
x
Y

P

P

G
|
G

V
x
G

H
x
A

N
x
S

T

M

E

E

L

I

Y

H

D

Q

E

A

L

L

N

T

S

R

S

S

E

S

T

S

V

I

T

R

P

N

V

V

L

L

V

P

T

R

H

H

E
|
E

G

Q

G

G

A

V

F

F

M

A

A

A

D

E

A

G

V

Y

S

A

R

R

A

S

D

G

D

K

T

P

V

-

G

G

A

I

L

C

A

I

I

A

V
x
T

P

S

A

G

A

P

G

G

I

A

T

T

H

N

A

L

A

V

S

S

G

G

I

L

G

A

E

D

S

A

F

L

L

L

G

D

G

S

I

V

P

P

M

L

L

V

I

A

F

I

S

T

G

G

G

Q

I

V

R

P

T

R

D

R

T

M

G

I

R

G

R

T

F

D

Q

A

L
x
F

H
x
Q

E
|
E

I

T

D

P

Q

I

L

V

R

E

L

V

I

T

E

R

P

S

L

I

T

T

K

K

A

H

A

N

F

Y

R

L

V

V

E

M

R

D

H

V

E

E

D

D

V

I

V

P

P

R

T

I

I

I

H

E

E

E

A

A

Y

F

R

F

I

L

A

A

T

T

G

S

G

G

Q
x
R

P

P

G

G

P

P

V

V

L

L

V

V

E

D

I

V

P

P

V
x
K

N

D

L

I

Q

Q

L

Q

-

Q

-

L

-

A

-

I

-

P

-

N

-

W

-

E

-

Q

-

A

-

M

-

R

F

L

P

P

G

G

E

Y

V

M

G

S

E

R

L

M

P

P

R

K

W

-

T

-

P

-

P

P

A

E

S

D

P

S

D

H

P

L

E

E

A

Q

V

I

R

V

R

R

A

-

D

-

L

L

L

I

L

S

A

E

A

S

R

K

P

P

G

V

L

L

F

Y

V

V

G

G

W
x
G

G
|
G

A

C

R

L

G

N

A

S

Q

S

A

D

E

E

V

L

A

G

A

R

I

F

A

V

E

E

F

L

L

T

Q

G

A

I

P

P

V

V

S

A

T

S

T
|
T

L
|
L

Q
x
M

G

G

L

L

A

G

S

S

F

Y

P

P

A

C

D

D

H

D

P

E

L

L

H

S

A

L

G

H

-

M

F
x
L

G

G

F
x
M

S
x
H

G

G

S

T

A

V

V

Y

P

-

A

-

A

A

R

N

N

Y

A

A

F

V

E

E

G

H

C

S

D

D

G

L

M

L

L

L

A

A

V

F

G
|
G

T
x
V

R
|
R

F

F

G
x
D

E
x
D

I
x
R

P
x
V

T

T

G

G

S

K

F

L

S

E

A

A

E

F

V

A

P

S

-

R

A

A

G

K

L

I

V

V

H

H

V

I

D
|
D

I
|
I

C

D

A

S

D

A

V

E

I

I

G

G

A

K

N

N

Y

K

P

T

A

P

A

H

V

V

G

S

I

V

V

C

G

G

D
|
D

A
x
V

R

K

T

L

V

A

L

L

A

Q

A

G

L

M

L

N

E

K

E

V

L

L

R

E

A

N

R

R

G

-

K

-

P

-

A

-

P

-

D

-

T

-

G

-

L

-

A

A

D

E

R

E

L

L

A

K

R

L

D

D

R

F

R

G

A

V

W

W

R

R

E

N

E

E

W

L

Y

N

A

V

H

Q

D

K

S

Q

K

K

G

F

R

P

V

L

N

S

P

F

A

K

R

T

F

F

F

G

D

E

E

A

L

I

R

P

R

-

Q

-

M

-

P

P

D

Q

D

Y

A

A

V

I

T

K

V

V

L

L

D

D

D

E

-

L

-

T

-

D

-

G

-

K

-

A

-

I

-

S

-

T

-

G

-
x
V

G

G

N

Q

H

H

T
x
Q

F
x
M

L

W

T

A

A

A

E

Q

L

F

F

Y

P

N

I

Y

H

K

R

K

G

P

G

R

R

Q

V

W

L

L

T

S

P

S

T
x
G

D
x
G

F

L

N
x
G

A

A

M
|
M

G

G

Y

F

A

G

V

L

P

P

A

A

I

I

G

G

A

A

K

S

L

V

A

A

Q

N

P

P

G

D

K

A

E

I

V

V

F

V

A

D

I

I

V

D

G

G

D
|
D

G

G

C

S

F

F

L

I

M

M

T

N

C

V

M

Q

E

E

I

L

M

A

T

T

A

I

T

R

A

V

R

E

G

N

L

L

G

P

V

V

I

K

Y

V

F

L

V

L

F

L

S

N

D
x
N

G

Q

E
x
H

L
|
L

S

G

Q
x
M

I
x
V

A

M

Q

Q

A
x
W

Q

E

E

D

I

R

P

F

Y

Y

N

K

active site: Y33 (≠ I24), G35 (= G26), G36 (≠ V27), A37 (≠ H28), S38 (≠ N29), E59 (= E50), T82 (≠ V74), F121 (≠ L113), Q122 (≠ H114), E123 (= E115), K171 (≠ V163), M266 (≠ F253), V293 (≠ P282), V400 (vs. gap), G426 (≠ N414), M428 (= M416), D453 (= D441), N480 (≠ D468), H482 (≠ E470), L483 (= L471), M485 (≠ Q473), V486 (≠ I474), W489 (≠ A477)
binding 2-[[[[(4-chloro-6-methoxy-2-pyrimidinyl)amino]carbonyl]amino]sulfonyl]benzoic acid ethyl ester: M266 (≠ F253), D291 (≠ E280), R292 (≠ I281), M485 (≠ Q473), W489 (≠ A477)
binding flavin-adenine dinucleotide: R161 (≠ Q153), G223 (≠ W211), G224 (= G212), T246 (= T234), L247 (= L235), M248 (≠ Q236), L264 (≠ F251), M266 (≠ F253), H267 (≠ S254), G286 (= G275), V287 (≠ T276), R288 (= R277), D290 (≠ G279), V293 (≠ P282), D310 (= D298), I311 (= I299), D329 (= D317), V330 (≠ A318), Q404 (≠ T392), M405 (≠ F393), G423 (≠ T411), G424 (≠ D412)
binding magnesium ion: D453 (= D441), N480 (≠ D468), H482 (≠ E470)

Sites not aligning to the query:

P09114 Acetolactate synthase 2, chloroplastic; ALS II; Acetohydroxy-acid synthase II; Acetolactate synthase II; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see paper)
26% identity, 93% coverage: 10:543/577 of query aligns to 98:640/664 of P09114

query
sites
P09114

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P191 (≠ R104) mutation to A: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with L-568.
W568 (≠ A477) mutation to L: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with A-191.

1t9cA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, sulfometuron methyl (see paper)
27% identity, 91% coverage: 13:539/577 of query aligns to 18:555/596 of 1t9cA

query
sites
1t9cA

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active site: Y29 (≠ I24), G31 (= G26), G32 (≠ V27), A33 (≠ H28), I34 (≠ N29), E55 (= E50), T78 (≠ V74), F117 (≠ L113), Q118 (≠ H114), E119 (= E115), K167 (≠ V163), R227 (≠ Q217), M263 (≠ F253), V290 (≠ P282), V406 (vs. gap), L431 (≠ F413), G432 (≠ N414), M434 (= M416), D459 (= D441), N486 (≠ D468), E488 (= E470), Q489 (≠ L471), M491 (≠ Q473), V492 (≠ I474), W495 (≠ A477), L517 (≠ V498), G522 (= G503), L523 (≠ A504)
binding methyl 2-[({[(4,6-dimethylpyrimidin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: G32 (≠ V27), V107 (≠ I103), P108 (≠ R104), F117 (≠ L113), K167 (≠ V163), D288 (≠ E280), R289 (≠ I281), W495 (≠ A477)
binding flavin-adenine dinucleotide: R157 (≠ Q153), G216 (= G210), A217 (≠ W211), G218 (= G212), N221 (vs. gap), T243 (= T234), L244 (= L235), Q245 (= Q236), L261 (≠ F251), M263 (≠ F253), H264 (≠ S254), G283 (= G275), A284 (≠ T276), R285 (= R277), D287 (≠ G279), R289 (≠ I281), V290 (≠ P282), E316 (≠ D298), V317 (≠ I299), N321 (≠ V303), G334 (= G316), D335 (= D317), A336 (= A318), M411 (≠ F393), G429 (≠ T411), G430 (≠ D412)
binding magnesium ion: D459 (= D441), N486 (≠ D468), E488 (= E470)

Sites not aligning to the query:

active site: 556

1t9dA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, metsulfuron methyl (see paper)
27% identity, 91% coverage: 13:539/577 of query aligns to 18:555/596 of 1t9dA

query
sites
1t9dA

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active site: Y29 (≠ I24), G31 (= G26), G32 (≠ V27), A33 (≠ H28), I34 (≠ N29), E55 (= E50), T78 (≠ V74), F117 (≠ L113), Q118 (≠ H114), E119 (= E115), K167 (≠ V163), R227 (≠ Q217), M263 (≠ F253), V290 (≠ P282), V406 (vs. gap), L431 (≠ F413), G432 (≠ N414), M434 (= M416), D459 (= D441), N486 (≠ D468), E488 (= E470), Q489 (≠ L471), M491 (≠ Q473), V492 (≠ I474), W495 (≠ A477), L517 (≠ V498), G522 (= G503), L523 (≠ A504)
binding methyl 2-[({[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: G32 (≠ V27), A33 (≠ H28), V107 (≠ I103), P108 (≠ R104), F117 (≠ L113), K167 (≠ V163), M263 (≠ F253), D288 (≠ E280), R289 (≠ I281), W495 (≠ A477)
binding flavin-adenine dinucleotide: R157 (≠ Q153), G216 (= G210), A217 (≠ W211), G218 (= G212), N221 (vs. gap), T243 (= T234), L244 (= L235), Q245 (= Q236), M260 (vs. gap), L261 (≠ F251), H264 (≠ S254), G283 (= G275), A284 (≠ T276), R285 (= R277), D287 (≠ G279), R289 (≠ I281), V290 (≠ P282), E316 (≠ D298), V317 (≠ I299), N321 (≠ V303), G334 (= G316), D335 (= D317), A336 (= A318), Q410 (≠ T392), M411 (≠ F393), G429 (≠ T411), G430 (≠ D412)
binding magnesium ion: D459 (= D441), N486 (≠ D468), E488 (= E470)
binding 2,5-dimethyl-pyrimidin-4-ylamine: E55 (= E50), P81 (≠ A77), Q118 (≠ H114), G432 (≠ N414), M434 (= M416), M464 (= M446)

Sites not aligning to the query:

active site: 556

Query Sequence

>WP_012565475.1 NCBI__GCF_000016185.1:WP_012565475.1
MKKTAAWLAVHAMEQLGIRFTFGIPGVHNTELYDELNSSETVTPVLVTHEGGGAFMADAV
SRAADDTVGALAIVPAAGITHAASGIGESFLGGIPMLIFSGGIRTDTGRRFQLHEIDQLR
LIEPLTKAAFRVERHEDVVPTIHEAYRIATGGQPGPVLVEIPVNLQLFPGEVGELPRWTP
PPPPASPDPEAVRRAADLLLAARRPGLFVGWGARGAQAEVAAIAEFLQAPVSTTLQGLAS
FPADHPLHAGFGFSGSAVPAARNAFEGCDGMLAVGTRFGEIPTGSFSAEVPAGLVHVDIC
ADVIGANYPAAVGIVGDARTVLAALLEELRARGKPAPDTGLADRLARDRRAWREEWYAHD
SKGRVNPARFFDELRRQMPDDAVTVLDDGNHTFLTAELFPIHRGGRVLTPTDFNAMGYAV
PAAIGAKLAQPGKEVFAIVGDGCFLMTCMEIMTATARGLGVIYFVFSDGELSQIAQAQEI
PYNRKPCTVLAGADLEGVARATGAAHVAMQNGDRIADAIAEARRLAATGRPVIVDVAIDY
SKRTAFTAGTAKSTFKRFPLSQRVRFAGRALLRRVTG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory