SitesBLAST
Comparing WP_012565752.1 NCBI__GCF_000016185.1:WP_012565752.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
3qhxA Crystal structure of cystathionine gamma-synthase metb (cgs) from mycobacterium ulcerans agy99 bound to hepes (see paper)
46% identity, 94% coverage: 20:378/383 of query aligns to 15:376/377 of 3qhxA
5x5hA Crystal structure of metb from corynebacterium glutamicum (see paper)
41% identity, 92% coverage: 30:381/383 of query aligns to 30:384/385 of 5x5hA
- active site: R52 (= R52), Y105 (= Y104), D177 (= D177), K202 (= K202)
- binding magnesium ion: F36 (= F36), Q38 (≠ R38), E49 (≠ S49), E69 (= E69), Q187 (≠ R187), P188 (= P188), L189 (≠ V189)
- binding pyridoxal-5'-phosphate: G80 (= G80), M81 (= M81), Y105 (= Y104), D177 (= D177), S199 (= S199), T201 (= T201), K202 (= K202)
4iyoD Crystal structure of cystathionine gamma lyase from xanthomonas oryzae pv. Oryzae (xometc) in complex with e-site serine, a-site serine, a- site external aldimine structure with aminoacrylate and a-site iminopropionate intermediates (see paper)
42% identity, 94% coverage: 20:378/383 of query aligns to 16:380/384 of 4iyoD
- active site: R47 (= R52), Y99 (= Y104), D172 (= D177), K197 (= K202)
- binding serine: Y45 (= Y50), T48 (≠ P53), Y99 (= Y104), Y99 (= Y104), R104 (= R108), K197 (= K202), N227 (≠ A232), E325 (≠ T331), S326 (= S332), T341 (≠ E347), R361 (= R359)
4iyoB Crystal structure of cystathionine gamma lyase from xanthomonas oryzae pv. Oryzae (xometc) in complex with e-site serine, a-site serine, a- site external aldimine structure with aminoacrylate and a-site iminopropionate intermediates (see paper)
42% identity, 94% coverage: 20:378/383 of query aligns to 16:380/381 of 4iyoB
- active site: R47 (= R52), Y99 (= Y104), D172 (= D177), K197 (= K202)
- binding 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid: Y45 (= Y50), R47 (= R52)
- binding amino-acrylate: Y99 (= Y104), K197 (= K202), S326 (= S332), T341 (≠ E347), R361 (= R359)
- binding pyruvic acid: Q221 (≠ R226), F224 (≠ A229)
- binding serine: Y45 (= Y50), T48 (≠ P53), Y99 (= Y104), R104 (= R108), N227 (≠ A232), E325 (≠ T331)
4iy7B Crystal structure of cystathionine gamma lyase (xometc) from xanthomonas oryzae pv. Oryzae in complex with e-site serine, a-site external aldimine structure with serine and a-site external aldimine structure with aminoacrylate intermediates (see paper)
42% identity, 94% coverage: 20:378/383 of query aligns to 16:380/381 of 4iy7B
- active site: R47 (= R52), Y99 (= Y104), D172 (= D177), K197 (= K202)
- binding 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid: Y45 (= Y50), R47 (= R52)
- binding (E)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-serine: G75 (= G80), M76 (= M81), Y99 (= Y104), E143 (= E148), N147 (= N152), D172 (= D177), S194 (= S199), K197 (= K202), S326 (= S332), L327 (= L333), T341 (≠ E347), R361 (= R359)
- binding pyruvic acid: Q221 (≠ R226), F224 (≠ A229)
- binding serine: Y45 (= Y50), T48 (≠ P53), Y99 (= Y104), R104 (= R108), N227 (≠ A232), E325 (≠ T331)
4iy7A Crystal structure of cystathionine gamma lyase (xometc) from xanthomonas oryzae pv. Oryzae in complex with e-site serine, a-site external aldimine structure with serine and a-site external aldimine structure with aminoacrylate intermediates (see paper)
42% identity, 94% coverage: 20:378/383 of query aligns to 16:380/381 of 4iy7A
- active site: R47 (= R52), Y99 (= Y104), D172 (= D177), K197 (= K202)
- binding 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid: G75 (= G80), M76 (= M81), Y99 (= Y104), E143 (= E148), N147 (= N152), D172 (= D177), S194 (= S199), K197 (= K202), S326 (= S332), L327 (= L333), T341 (≠ E347), R361 (= R359)
- binding (E)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-serine: Y45 (= Y50), R47 (= R52)
- binding serine: Y45 (= Y50), T48 (≠ P53), Y99 (= Y104), R104 (= R108), N227 (≠ A232), E325 (≠ T331)
4ixzA Native structure of cystathionine gamma lyase (xometc) from xanthomonas oryzae pv. Oryzae at ph 9.0 (see paper)
42% identity, 94% coverage: 20:378/383 of query aligns to 16:380/381 of 4ixzA
4ixsB Native structure of xometc at ph 5.2 (see paper)
41% identity, 94% coverage: 20:378/383 of query aligns to 15:371/372 of 4ixsB
6cjaA Crystal structure of cystathionine beta-lyase from legionella pneumophila philadelphia 1 in complex with alanyl-plp and serine
39% identity, 97% coverage: 6:378/383 of query aligns to 2:380/381 of 6cjaA
- binding (E)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-alanine: Y45 (= Y50), R47 (= R52), S74 (= S79), G75 (= G80), M76 (= M81), Y99 (= Y104), N147 (= N152), D172 (= D177), S194 (= S199), T196 (= T201), K197 (= K202), S326 (= S332), T341 (≠ E347), R361 (= R359)
- binding serine: E44 (≠ S49), R47 (= R52), T48 (≠ P53), R104 (= R108), E325 (≠ T331)
4l0oH Structure determination of cystathionine gamma-synthase from helicobacter pylori
40% identity, 95% coverage: 17:381/383 of query aligns to 11:373/373 of 4l0oH
- active site: R40 (= R52), Y92 (= Y104), D164 (= D177), K189 (= K202)
- binding pyridoxal-5'-phosphate: Y38 (= Y50), R40 (= R52), S67 (= S79), G68 (= G80), L69 (≠ M81), Y92 (= Y104), D164 (= D177), S186 (= S199), T188 (= T201), K189 (= K202)
6k1lB E53a mutant of a putative cystathionine gamma-lyase (see paper)
41% identity, 94% coverage: 20:379/383 of query aligns to 17:382/382 of 6k1lB
- binding pyridoxal-5'-phosphate: Y46 (= Y50), R48 (= R52), S75 (= S79), G76 (= G80), M77 (= M81), Y100 (= Y104), D173 (= D177), S195 (= S199), K198 (= K202)
- binding pyruvic acid: Y100 (= Y104), K198 (= K202), E326 (≠ T331), T342 (≠ E347), R362 (= R359)
6k1lA E53a mutant of a putative cystathionine gamma-lyase (see paper)
41% identity, 94% coverage: 20:379/383 of query aligns to 17:382/382 of 6k1lA
7ba4A Structure of cystathionine gamma-lyase from pseudomonas aeruginosa
39% identity, 97% coverage: 10:381/383 of query aligns to 9:374/377 of 7ba4A
1pg8A Crystal structure of l-methionine alpha-, gamma-lyase
40% identity, 94% coverage: 19:379/383 of query aligns to 19:395/398 of 1pg8A
- active site: R61 (= R52), Y114 (= Y104), D186 (= D177), K211 (= K202)
- binding pyridoxal-5'-phosphate: Y59 (= Y50), R61 (= R52), S88 (= S79), G89 (= G80), M90 (= M81), Y114 (= Y104), D186 (= D177), S208 (= S199), T210 (= T201), K211 (= K202)
P13254 L-methionine gamma-lyase; MGL; Homocysteine desulfhydrase; L-methioninase; EC 4.4.1.11; EC 4.4.1.2 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 3 papers)
40% identity, 94% coverage: 19:379/383 of query aligns to 19:395/398 of P13254
- YSR 59:61 (≠ YAR 50:52) binding pyridoxal 5'-phosphate
- R61 (= R52) mutation R->A,E,F: Loss of elimination activity against L-methionine.
- GM 89:90 (= GM 80:81) binding in other chain
- Y114 (= Y104) binding substrate
- C116 (≠ G106) mutation to H: Drastic decrease of the catalytic efficiency of the elimination reaction with L-methionine, by 6700-fold, and increases that with L-cysteine by 7-fold, mainly due to changes in kcat. Loss of ability to catalyze replacement reaction between L-methionine and 2-mercaptoethanol.; mutation to S: 9% of wild-type elimination activity against L-methionine.; mutation to T: 40% of wild-type elimination activity against L-methionine.
- SAT 208:210 (= SAT 199:201) binding in other chain
- K211 (= K202) modified: N6-(pyridoxal phosphate)lysine
- K240 (≠ R231) mutation K->D,E: Marked decrease in elimination activity against both L-methionine and DL-homocysteine.; mutation to M: 50% reduction in alpha,gamma-elimination activity against DL-homocysteine, while retaining elimination activity against L-methionine and L-cysteine.
- D241 (≠ A232) mutation D->H,R: 5 to 14-fold reduction in alpha,gamma-elimination activity against L-methionine, while no change in affinity for L-methionine.
- R375 (= R359) binding substrate
3vk3A Crystal structure of l-methionine gamma-lyase from pseudomonas putida c116h mutant complexed with l-methionine (see paper)
40% identity, 94% coverage: 19:379/383 of query aligns to 18:394/397 of 3vk3A
5x30C Crystal structure of pseudomonas putida methionine gamma-lyase c116h mutant with l-homocysteine intermediates. (see paper)
40% identity, 94% coverage: 19:379/383 of query aligns to 14:390/393 of 5x30C
5x2xA Crystal structure of pseudomonas putida methionine gamma-lyase wild type with l-homocysteine intermediates (see paper)
40% identity, 94% coverage: 19:379/383 of query aligns to 13:389/392 of 5x2xA
- active site: R55 (= R52), Y108 (= Y104), D180 (= D177), K205 (= K202)
- binding (2E)-2-{[(1E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}but-2-enoic acid: Y53 (= Y50), R55 (= R52), G83 (= G80), M84 (= M81), Y108 (= Y104), N155 (= N152), D180 (= D177), S202 (= S199), T204 (= T201), K205 (= K202), V333 (≠ T331), S334 (= S332), R369 (= R359)
5x2wA Crystal structure of pseudomonas putida methionine gamma-lyase wild type with l-methionine intermediates (see paper)
40% identity, 94% coverage: 19:379/383 of query aligns to 13:389/392 of 5x2wA
- active site: R55 (= R52), Y108 (= Y104), D180 (= D177), K205 (= K202)
- binding (2E)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]-4-(methylsulfanyl)but-2-enoic acid: Y53 (= Y50), R55 (= R52), S82 (= S79), G83 (= G80), M84 (= M81), Y108 (= Y104), D180 (= D177), S202 (= S199), K205 (= K202), V333 (≠ T331), S334 (= S332), R369 (= R359)
8j6nA Crystal structure of cystathionine gamma-lyase in complex with compound 1 (see paper)
38% identity, 93% coverage: 22:379/383 of query aligns to 24:386/390 of 8j6nA
- binding [6-methyl-4-[(~{E})-(oxamoylhydrazinylidene)methyl]-5-oxidanyl-pyridin-3-yl]methyl dihydrogen phosphate: Y51 (= Y50), R53 (= R52), G81 (= G80), L82 (≠ M81), Y105 (= Y104), E148 (= E148), N152 (= N152), D178 (= D177), S200 (= S199), T202 (= T201), K203 (= K202), E330 (≠ T331), S331 (= S332), T346 (≠ E347), R366 (= R359)
Query Sequence
>WP_012565752.1 NCBI__GCF_000016185.1:WP_012565752.1
MRPDPTHPDTLLARAQGAHDPATHGVVPAVHPATTFLRAGDLSYPGGHSYARPFNPTFDG
AETLLATLEGAAQALLFSSGMSAATAVFQALEPGDHVIAPQVMYWGLRNWLKGFAAQWGL
GLDLVDMRDPAAVAAAVRPGRTRLIWAETPANPTWEVTDLAACAEIAHRAGARLAVDNTV
PTPLLTRPVEHGADIVMHSATKYLNGHSDVLGGALATARADGFWERIAAIRANQGAIPGP
FEAWLLLRGMRTLGLRVERACATAQRLAEHFAGHPKLEAVLYPGLPTHPGHAVAARQMRG
GFGGMLSVRVKGGAAGAVAASAALRIWTRATSLGGVESLVEHRGSIEGPDSPCPQDLLRL
SCGIEHADDLIADLEQALEHVPA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory