SitesBLAST
Comparing WP_012566497.1 NCBI__GCF_000016185.1:WP_012566497.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q5F8J4 Homoserine dehydrogenase; HDH; HSD; NAD(+)-dependent homoserine dehydrogenase; NAD(+)-dependent HSD; NgHSD; EC 1.1.1.3 from Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) (see paper)
47% identity, 100% coverage: 3:430/430 of query aligns to 2:433/435 of Q5F8J4
- L45 (≠ R46) mutation to R: Shows a marked increase in the catalytic efficiency with NADP(+).
- LS 45:46 (≠ RS 46:47) mutation to RD: Does not impair the catalytic activity with NAD(+). Slightly increases the activity, but slightly decreases the affinity for NADP(+).; mutation to RR: Causes a shift in coenzyme preference from NAD(+) to NADP(+) by a factor of 974. Shows a slight decrease in the catalytic efficiency with NAD(+) and a 4.5-fold increase in catalytic efficiency with NADP(+).
6dzsA Mycobacterial homoserine dehydrogenase thra in complex with NADP
40% identity, 99% coverage: 3:428/430 of query aligns to 3:429/431 of 6dzsA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G11), L12 (= L12), G13 (= G13), N14 (≠ T14), V15 (= V15), V45 (≠ A45), R46 (= R46), R47 (≠ S47), R52 (= R52), I63 (≠ A65), L78 (= L80), M79 (≠ I81), P84 (≠ G86), A102 (= A105), K104 (= K107), G306 (= G304), T310 (= T308)
4pg7A Crystal structure of s. Aureus homoserine dehydrogenase at ph7.5 (see paper)
35% identity, 79% coverage: 3:341/430 of query aligns to 3:331/402 of 4pg7A
Sites not aligning to the query:
6a0tB Homoserine dehydrogenase k99a mutant from thermus thermophilus hb8 complexed with hse and NADP+ (see paper)
39% identity, 58% coverage: 5:252/430 of query aligns to 4:240/332 of 6a0tB
- active site: D191 (= D203), K195 (= K207)
- binding l-homoserine: N150 (= N161), G151 (= G162), T152 (= T163), Y178 (= Y190), E180 (= E192), D186 (= D198), K195 (= K207)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G11), G11 (≠ L12), G12 (= G13), T13 (= T14), V14 (= V15), L42 (≠ S44), V43 (≠ A45), R44 (= R46), D45 (≠ S47), K48 (= K50), R50 (= R52), A73 (≠ L80), M74 (≠ I81), G75 (= G82), A97 (= A105), N98 (= N106), G177 (= G189), E180 (= E192)
Sites not aligning to the query:
6a0sA Homoserine dehydrogenase from thermus thermophilus hb8 complexed with hse and NADPH (see paper)
39% identity, 58% coverage: 5:252/430 of query aligns to 4:240/331 of 6a0sA
- active site: D191 (= D203), K195 (= K207)
- binding l-homoserine: K99 (= K107), N150 (= N161), G151 (= G162), T152 (= T163), Y178 (= Y190), E180 (= E192), D186 (= D198), K195 (= K207)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (≠ L12), G12 (= G13), T13 (= T14), V14 (= V15), L42 (≠ S44), V43 (≠ A45), R44 (= R46), D45 (≠ S47), K48 (= K50), R50 (= R52), A73 (≠ L80), M74 (≠ I81), A97 (= A105), K99 (= K107), G177 (= G189), E180 (= E192)
Sites not aligning to the query:
2ejwA Homoserine dehydrogenase from thermus thermophilus hb8
39% identity, 58% coverage: 5:252/430 of query aligns to 4:240/331 of 2ejwA
7f4cA The crystal structure of the immature holo-enzyme of homoserine dehydrogenase complexed with NADP and 1,4-butandiol from the hyperthermophilic archaeon sulfurisphaera tokodaii. (see paper)
31% identity, 73% coverage: 5:317/430 of query aligns to 1:293/300 of 7f4cA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: F6 (≠ A10), G7 (= G11), Y8 (≠ L12), G9 (= G13), N10 (≠ T14), V11 (= V15), T37 (≠ V39), R38 (≠ V40), R39 (≠ T41), V72 (≠ L80), S73 (≠ I81), S74 (≠ G82), T100 (≠ A105), K102 (= K107), G127 (≠ A132), S131 (≠ G136), E185 (= E192), G280 (= G306), A284 (≠ S310)
F9VNG5 Homoserine dehydrogenase; HDH; HSD; StHSD; EC 1.1.1.3 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 3 papers)
32% identity, 73% coverage: 5:317/430 of query aligns to 1:297/304 of F9VNG5
- Y8 (≠ L12) binding NADP(+)
- N10 (≠ T14) binding NADP(+)
- V11 (= V15) binding NADP(+)
- R38 (≠ V40) binding NADP(+)
- R39 (≠ T41) binding NADP(+)
- S73 (≠ V77) binding NADP(+)
- T100 (≠ A105) binding NADP(+)
- K102 (= K107) binding NADP(+)
- G182 (= G189) binding NADP(+)
- E185 (= E192) binding NADP(+)
- G284 (= G306) binding NADP(+)
Sites not aligning to the query:
5x9dA Crystal structure of homoserine dehydrogenase in complex with l- cysteine and NAD (see paper)
31% identity, 73% coverage: 5:317/430 of query aligns to 1:295/302 of 5x9dA
- active site: D196 (= D203), K200 (= K207)
- binding (2R)-3-[[(4S)-3-aminocarbonyl-1-[(2R,3R,4S,5R)-5-[[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-3,4-bis(oxidanyl)oxolan-2-yl]-4H-pyridin-4-yl]sulfanyl]-2-azanyl-propanoic acid: F6 (≠ A10), G7 (= G11), Y8 (≠ L12), G9 (= G13), N10 (≠ T14), V11 (= V15), T37 (≠ V39), R38 (≠ V40), V72 (= V76), S73 (≠ V77), S74 (≠ C78), P82 (≠ G86), T100 (≠ A105), N101 (= N106), K102 (= K107), G127 (≠ A132), S131 (≠ G136), N155 (= N161), G156 (= G162), T157 (= T163), Y183 (= Y190), A184 (= A191), E185 (= E192), D191 (= D198), D196 (= D203), K200 (= K207), A281 (= A305), G282 (= G306), A286 (≠ S310)
3ingA Crystal structure of homoserine dehydrogenase (np_394635.1) from thermoplasma acidophilum at 1.95 a resolution
32% identity, 53% coverage: 3:230/430 of query aligns to 1:236/319 of 3ingA
- active site: D209 (= D203), K213 (= K207)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G9 (= G11), T10 (≠ L12), G11 (= G13), N12 (≠ T14), V13 (= V15), D38 (≠ S44), S39 (≠ A45), K57 (≠ D63), C85 (≠ L80), T86 (≠ I81), P87 (≠ G82), A112 (= A105), N113 (= N106), K114 (= K107), A139 (= A132), E198 (= E192), S199 (≠ A193)
Sites not aligning to the query:
4xb1A Hyperthermophilic archaeal homoserine dehydrogenase in complex with NADPH (see paper)
26% identity, 73% coverage: 5:317/430 of query aligns to 3:309/319 of 4xb1A
- active site: D211 (= D203), K215 (= K207)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F8 (≠ A10), F10 (≠ L12), G11 (= G13), T12 (= T14), V13 (= V15), D39 (≠ A45), R40 (= R46), K57 (= K50), V91 (≠ L80), S92 (≠ I81), S93 (≠ G82), S114 (≠ A105), K116 (= K107), S141 (≠ A132), G295 (≠ A303), T300 (= T308)
O58802 Homoserine dehydrogenase; HDH; HSD; HseDH; EC 1.1.1.3 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see paper)
26% identity, 73% coverage: 5:317/430 of query aligns to 3:309/319 of O58802
- F10 (≠ L12) binding NADPH
- T12 (= T14) binding NADPH
- V13 (= V15) binding NADPH
- R40 (= R46) binding NADPH; mutation to A: Increases activity with NADP.
- K57 (= K50) binding NADPH; mutation to A: Increases activity with NADP.
- S92 (≠ I81) binding NADPH
- S93 (≠ G82) binding NADPH
- S114 (≠ A105) binding NADPH
- K116 (= K107) binding NADPH
- E140 (= E131) binding Na(+)
- V143 (= V134) binding Na(+)
- A145 (≠ G136) binding Na(+)
- T147 (≠ I138) binding Na(+)
- G296 (= G304) binding NADPH
4xb2A Hyperthermophilic archaeal homoserine dehydrogenase mutant in complex with NADPH (see paper)
30% identity, 53% coverage: 88:317/430 of query aligns to 97:309/319 of 4xb2A
Sites not aligning to the query:
3jsaA Homoserine dehydrogenase from thermoplasma volcanium complexed with NAD
32% identity, 53% coverage: 3:230/430 of query aligns to 2:239/321 of 3jsaA
- active site: D212 (= D203), K216 (= K207)
- binding nicotinamide-adenine-dinucleotide: G10 (= G11), L11 (= L12), G12 (= G13), N13 (≠ T14), V14 (= V15), D42 (≠ V43), S43 (= S44), A90 (≠ L80), T91 (≠ I81), P92 (≠ G82), A117 (= A105), N118 (= N106), A144 (= A132)
Sites not aligning to the query:
O94671 Homoserine dehydrogenase; HDH; HSD; EC 1.1.1.3 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
26% identity, 52% coverage: 5:229/430 of query aligns to 8:245/376 of O94671
- S201 (≠ D177) modified: Phosphoserine
1tveA Homoserine dehydrogenase in complex with 4-(4-hydroxy-3- isopropylphenylthio)-2-isopropylphenol (see paper)
28% identity, 51% coverage: 2:221/430 of query aligns to 2:235/358 of 1tveA
1q7gA Homoserine dehydrogenase in complex with suicide inhibitor complex NAD-5-hydroxy-4-oxonorvaline (see paper)
28% identity, 51% coverage: 2:221/430 of query aligns to 2:235/358 of 1q7gA
- active site: D218 (= D203), K222 (= K207)
- binding nicotinamide-adenine-dinucleotide-5-hydroxy-4-oxonorvaline: G13 (= G13), V14 (≠ T14), V15 (= V15), E39 (≠ R33), N91 (≠ G82), T92 (≠ G83), S93 (= S84), I97 (≠ A88), P114 (≠ A105), K116 (= K107), A143 (= A132), S173 (≠ N161), K222 (= K207)
Sites not aligning to the query:
1ebuD Homoserine dehydrogenase complex with NAD analogue and l-homoserine (see paper)
28% identity, 51% coverage: 2:221/430 of query aligns to 2:235/358 of 1ebuD
- active site: D218 (= D203), K222 (= K207)
- binding 3-aminomethyl-pyridinium-adenine-dinucleotide: G11 (= G11), A12 (≠ L12), G13 (= G13), V14 (≠ T14), V15 (= V15), E39 (≠ R33), A40 (≠ C34), N91 (≠ G82), S93 (= S84), K116 (= K107)
Sites not aligning to the query:
1ebfA Homoserine dehydrogenase from s. Cerevisiae complex with NAD+ (see paper)
28% identity, 51% coverage: 2:221/430 of query aligns to 2:235/358 of 1ebfA
- active site: D218 (= D203), K222 (= K207)
- binding nicotinamide-adenine-dinucleotide: I10 (≠ A10), A12 (≠ L12), G13 (= G13), V14 (≠ T14), V15 (= V15), E39 (≠ R33), A40 (≠ C34), T92 (≠ G83), S93 (= S84), P114 (≠ A105)
P31116 Homoserine dehydrogenase; HDH; HSD; EC 1.1.1.3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
28% identity, 51% coverage: 2:221/430 of query aligns to 3:236/359 of P31116
- A13 (≠ L12) binding NAD(+)
- V15 (≠ T14) binding NAD(+)
- V16 (= V15) binding NAD(+)
- A41 (≠ C34) binding NAD(+)
- H79 (≠ A67) mutation to A: Reduces kcat 2-fold.
- T93 (≠ G83) binding NAD(+)
- E143 (= E131) binding Na(+)
- V146 (= V134) binding Na(+)
- A148 (≠ G136) binding Na(+)
- L150 (≠ I138) binding Na(+)
- E208 (= E192) binding L-homoserine; mutation to D: Increases KM for aspartate-semialdehyde 48-fold and reduces kcat by 50%.; mutation E->L,Q: Loss of activity.
- D219 (= D203) binding L-homoserine; mutation to L: Reduces kcat 150-fold.
- K223 (= K207) mutation to V: Loss of activity.
Sites not aligning to the query:
- 309 H→A: Reduces kcat 40-fold. Affects dimer formation.
- 340 binding NAD(+)
Query Sequence
>WP_012566497.1 NCBI__GCF_000016185.1:WP_012566497.1
MAKPLKIGIAGLGTVGGGVLQILRRNADLLETRCGRPVVVTAVSARSRGKDRGADLSGLR
WYDDAAALAHDPEVDVVCELIGGSDGVAKALVETALANGRHVVTANKALLARHGTALALA
AESRGLTLAYEAAVAGGIPVIKGLREGLAANRVREVHGILNGTCNYILTEMRTTGRDFAD
VLADAQRLGYAEADPGFDIDGVDAAHKLAILTAVAFGCQVDFDAVHVEGIRHISSLDIQY
ADELGYRIRLLAIGRRTERGIEQRVHPCMVPVGSPIGATDGVFNAVVADGDFVDKVVLVG
RGAGAGPTASAVVADLLDIAQGRRTPTFGVPAERLVPLPASPIDSRRGRYYLRLMVVDRP
GVIADVAALLRDHNVSMEAFLQRGRAPGEAVPVVLTTHETDEAAMQRALAQIGALDTVLE
PPRLIRIEDF
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory