SitesBLAST

P46154 Glutathione-independent formaldehyde dehydrogenase; FALDH; FDH; Formaldehyde dismutase; EC 1.2.1.46; EC 1.2.98.1 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see paper)
36% identity, 96% coverage: 2:379/395 of query aligns to 5:380/399 of P46154

query
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P46154

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C47 (= C38) binding Zn(2+)
G48 (= G39) binding NAD(+)
S49 (= S40) binding NAD(+)
H52 (= H43) binding NAD(+)
H68 (= H60) binding Zn(2+)
C98 (= C90) binding Zn(2+)
C101 (= C93) binding Zn(2+)
C104 (= C96) binding Zn(2+)
C112 (= C104) binding Zn(2+)
V198 (= V201) binding NAD(+)
D218 (= D221) binding NAD(+)
R223 (= R226) binding NAD(+)
V263 (= V265) binding NAD(+)
R268 (≠ H270) binding NAD(+)
H270 (≠ T272) binding NAD(+)
P300 (= P312) binding NAD(+)
L302 (≠ V314) binding NAD(+)
G337 (= G337) binding NAD(+)
T339 (= T339) binding NAD(+)

5ylnA Zinc dependent alcohol dehydrogenase 2 from streptococcus pneumonia - apo form
30% identity, 95% coverage: 17:392/395 of query aligns to 21:342/348 of 5ylnA

query
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5ylnA

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active site: C41 (= C38), T43 (≠ S40), H46 (= H43), H63 (= H60)
binding zinc ion: C41 (= C38), H63 (= H60), C93 (= C90), C96 (= C93), C99 (= C96), C107 (= C104)

7xy9A Cryo-em structure of secondary alcohol dehydrogenases tbsadh after carrier-free immobilization based on weak intermolecular interactions (see paper)
29% identity, 93% coverage: 25:391/395 of query aligns to 25:342/344 of 7xy9A

query
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7xy9A

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binding magnesium ion: H101 (≠ F118), H103 (≠ K120), H158 (≠ M180), C288 (≠ F334), G290 (≠ M336), G291 (= G337), L293 (≠ T339), R294 (≠ H340)
binding zinc ion: C38 (= C38), H60 (= H60), E61 (= E61), D151 (= D173)

2ouiA D275p mutant of alcohol dehydrogenase from protozoa entamoeba histolytica (see paper)
30% identity, 93% coverage: 25:392/395 of query aligns to 24:350/360 of 2ouiA

query
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2ouiA

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N

-

S

-

N

T

P

P

N
x
D

A

W

G

G

V
x
E

A

E

E

E

T
x
S

I

Q

F

R

G

G

-

Y

-

P

-

M

K

H

S
|
S

P

G

S

G

A

M

L

L

F

G

G

G

Y

W

S

K

F

F

L

-

M

-

G

S

G

N

F

F

A

K

G

D

G

G

Q

V

-

F

A

S

E

E

Y

V

V

F

R

H

V

V

P

N

Y

E

A

A

D

D

V

A

G

N

P

L

M

A

K

L

I

L

P

P

H

-

V

-

R

R

D

D

G

I

G

K

P

P

E

E

D

D

D

-

K

-

V

A

L

V

F

M

L

L

T

S

D
|
D

I

M

F

V

P

T

T
|
T

G

G

W

F

M

H

A

G

A

A

E

E

N

L

C

A

A

N

I

I

Q

K

D

L

G

G

D

D

V

T

V

V

A

C

V

V

W

I

G

G

C

I

G

G

P

P

V

V

G

G

Q

L

F

M

A

S

I

V

R

A

S

G

A

A

Q

N

L

H

Q

L

G

G

A

A

A

G

R

R

V

I

V

F

A

A

I

V

D

G

R

S

V

R

P

K

E

H

R

C

L

C

R

D

L

I

A

A

A

L

E

E

A

Y

G

G

A

A

-

T

E

D

T

I

I

I

D

N

F

Y

D

K

R

N

N

G

D

D

V

I

R

V

A

E

R

Q

L

I

D

L

E

K

L

A

T

T

G

D

G

G

R

K

G

G

P

V

D

D

S

K

C

V

I

V

N

I

A

A

V

G

G

G

I

-

E

-

A

-

H

-

G

-

T

-

G

-

S

-

V

-

D

-

A

-

V

-

L

-

D

-

R

-

A

-

K

-

T

-

A

-

V

-

G

-

L

-

A

-

T

-

D

-

R

D

G

V

H

H

V

T

L

F

R

A

Q

Q

A

A

I

V

M

K

A

M

C

I

R

K

K

P

G

G

G

S

T

D

I

I

S

G

I

N

P

V

G

N

V

-

Y

Y

A

L

G

G

V

E

L

G

D

D

K

N

V

I

P

P

F

I

G

P

A

R

A

S

F

E

A

-

K

-

G

-

L

W

N

G

F

V

R

G

M

M

G

G

Q

H

T

K

H

H

M

I

M

H

R

G

Y

G

M

L

R

T

P

P

-

G

-

R

-

V

-

R

-

M

-

E

-

K

L

L

L

A

E

S

R

L

I

I

E

S

K

T

G

G

E

K

I

L

D

D

P

T

S

S

F

K

L

L

I

I

T

T

H

H

R

R

L

F

S

E

-

G

L

L

E

E

D

K

G

V

P

E

G

D

A

A

Y

L

R

M

T

L

F

M

R

K

D

N

K

K

Q

P

D

A

G

D

C

L

I

I

K
|
K

V

P

V

V

M

V

R

R

active site: C37 (= C38), T38 (≠ G39), S39 (= S40), H42 (= H43), H59 (= H60), E60 (= E61), D89 (≠ N110), E92 (≠ V113), S95 (≠ T116), S103 (= S121), D150 (= D173), T154 (= T177), K346 (= K388)
binding zinc ion: C37 (= C38), H59 (= H60), D150 (= D173)

2dfvA Hyperthermophilic threonine dehydrogenase from pyrococcus horikoshii (see paper)
26% identity, 99% coverage: 1:392/395 of query aligns to 7:346/346 of 2dfvA

query
sites
2dfvA

M

M

K

K

A

T

L

K

C

P

W

G

H

Y

G

G

K

A

E

E

D

L

V

V

R

E

V

V

E

D

R

-

V

V

P

P

D

K

P

P

R

G

I

-

A

-

E

-

P

P

G

G

D

E

I

V

I

L

V

I

K

K

V

V

T

L

S

A

T

T

A

S

I

I

C
|
C

G
|
G

S
x
T

D

D

L

L

H
|
H

L

I

Y

Y

D

E

G

W

Y

N

-

E

-

W

-

A

I

Q

P

S

T

R

M

I

R

K

Q

P

G

P

D

Q

I

I

L

M

G

G

H
|
H

E
|
E

F

V

M

A

G

G

E

E

V

V

E

E

T

I

G

G

R

P

E

G

V

V

K

E

T

G

L

I

K

E

V

V

G

G

D

D

R

Y

V

V

I

S

V

V

P

E

F

T

T

H

I

I

A

V

C
|
C

G

G

G

K

C
|
C

F

Y

F

A

C
|
C

N

R

R

R

Q

G

L

Q

W

Y

S

H

L

V

C
|
C

D

Q

N

N

S

T

N
x
K

P

-

N

-

A

-

G

-

V

-

A

-

E

-

T

-

I

I

F

F

G

G

K

V

S

D

P

T

S

D

A

G

L

V

F

F

G

-

Y

-

S

-

F

-

L

-

M

-

G

-

F

-

A

-

G

-

Q

-

A

A

E

E

Y

Y

V

A

R

V

V

V

P

P

Y

A

A

Q

D

N

V

I

-

W

-

K

G

N

P

P

M

K

K

S

I

I

P

P

H

-

V

-

R

-

D

-

G

-

P

P

E

E

D

-

K

-

V

-

L

-

F

Y

L

A

T

T

D

L

I

Q

F

E

P
|
P

T

L

G

G

W

N

M
x
A

A

V

A

D

E

T

N

V

C

L

A

A

-

G

I

P

Q

I

D

S

G

G

D

K

V

S

V

V

A

L

V

I

W

T

G

G

C

A

G
|
G

P
|
P

V
x
L

G

G

Q

L

F

L

A

G

I

I

R

A

S

V

A

A

Q

K

L

A

Q

S

G

G

A

A

A

Y

R

P

V

V

V

I

A

V

I

S

D
x
E

R
x
P

V

S

P

D

E

F

R
|
R

L

R

R

E

L

L

A

A

A

K

E

K

A

V

G

G

A

A

E

D

-

Y

T

V

I

I

D

N

F

P

D

F

R

E

N

E

D

D

V

V

R

V

A

K

R

E

L

V

D

M

E

D

L

I

T

T

G

D

G

G

R

N

G

G

P

V

D

D

S

V

C

F

I

L

N

E

A
x
F

V
x
S

G

G

I
x
A

E

P

A

-

H

-

G

-

T

-

G

-

S

-

V

-

D

-

A

-

V

-

L

-

D

-

R

-

A

-

K

-

T

-

A

-

V

-

G

-

L

-

A

-

T

-

D

-

R

-

G

-

H

K

V

A

L

L

R

E

Q

Q

A

G

I

L

M

Q

A

A

C

V

R

T

K

P

G

A

G

G

T

R

I

V

S

S

I

L

P
x
L

G
|
G

V
x
L

Y

Y

A

P

G

G

V

K

L

V

-

T

-

I

-

D

-

F

-

N

D

N

K

L

V

I

P

I

F

F

G

K

A

A

A

L

F

T

A

I

K

Y

G

G

L
x
I

N
x
T

F

G

R

R

M

-

G

-

Q

-

T

-

H

H

M

L

M

W

R

E

Y

T

M

W

R

Y

P

T

L

V

L

S

E

R

R

L

I

L

E

Q

K

S

G

G

E

K

I

L

D

N

P

L

S

D

F

P

L

I

I

I

T

T

H

H

R

K

L

Y

S

K

-

G

L

F

E

D

D

K

G

Y

P

E

G

E

A

A

Y

F

R

E

T

L

F

M

R

R

D

A

K

G

Q

K

D

T

G

G

C
x
K

I

V

K

V

V

F

V

M

M

L

R

K

active site: C40 (= C38), G41 (= G39), T42 (≠ S40), H45 (= H43), H65 (= H60), E66 (= E61), C95 (= C90), C98 (= C93), C101 (= C96), C109 (= C104), K113 (≠ N108), P151 (= P176), A155 (≠ M180), K340 (≠ C386)
binding nicotinamide-adenine-dinucleotide: G175 (= G199), P176 (= P200), L177 (≠ V201), E197 (≠ D221), P198 (≠ R222), R202 (= R226), F241 (≠ A264), S242 (≠ V265), A244 (≠ I267), L264 (≠ P312), G265 (= G313), L266 (≠ V314), I289 (≠ L332), T290 (≠ N333)
binding zinc ion: C95 (= C90), C101 (= C96), C109 (= C104)

O58389 L-threonine 3-dehydrogenase; L-ThrDH; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 2 papers)
26% identity, 99% coverage: 1:392/395 of query aligns to 9:348/348 of O58389

query
sites
O58389

M

M

K

K

A

T

L

K

C

P

W

G

H

Y

G

G

K

A

E

E

D

L

V

V

R

E

V

V

E

D

R

-

V

V

P

P

D

K

P

P

R

G

I

-

A

-

E

-

P

P

G

G

D

E

I

V

I

L

V

I

K

K

V

V

T

L

S

A

T

T

A

S

I

I

C
|
C

G

G

S
x
T

D

D

L

L

H

H

L

I

Y

Y

D

E

G

W

Y

N

-

E

-

W

-

A

I

Q

P

S

T

R

M

I

R

K

Q

P

G

P

D

Q

I

I

L

M

G

G

H
|
H

E
|
E

F

V

M

A

G

G

E

E

V

V

E

E

T

I

G

G

R

P

E

G

V

V

K

E

T

G

L

I

K

E

V

V

G

G

D

D

R

Y

V

V

I

S

V

V

P

E

F

T

T

H

I

I

A

V

C
|
C

G

G

G

K

C
|
C

F

Y

F

A

C
|
C

N

R

R

R

Q

G

L

Q

W

Y

S

H

L

V

C
|
C

D

Q

N

N

S

T

N

K

P

-

N

-

A

-

G

-

V

-

A

-

E

-

T

-

I

I

F

F

G

G

K

V

S

D

P

T

S

D

A

G

L

V

F

F

G

-

Y

-

S

-

F

-

L

-

M

-

G

-

F

-

A

-

G

-

Q

-

A

A

E

E

Y

Y

V

A

R

V

V

V

P

P

Y

A

A

Q

D

N

V

I

-

W

-

K

G

N

P

P

M

K

K

S

I

I

P

P

H

-

V

-

R

-

D

-

G

-

P

P

E

E

D

-

K

-

V

-

L

-

F

Y

L

A

T

T

D

L

I

Q

F
x
E

P

P

T

L

G

G

W

N

M

A

A

V

A

D

E

T

N

V

C

L

A

A

-

G

I

P

Q

I

D

S

G

G

D

K

V

S

V

V

A

L

V

I

W

T

G

G

C

A

G

G

P

P

V
x
L

G

G

Q

L

F

L

A

G

I

I

R

A

S

V

A

A

Q

K

L

A

Q

S

G

G

A

A

A

Y

R

P

V

V

V

I

A

V

I

S

D
x
E

R

P

V

S

P

D

E

F

R
|
R

L

R

R

E

L

L

A

A

A

K

E

K

A

V

G

G

A

A

E

D

-

Y

T

V

I

I

D

N

F

P

D

F

R

E

N

E

D

D

V

V

R

V

A

K

R

E

L

V

D

M

E

D

L

I

T

T

G

D

G

G

R

N

G

G

P

V

D

D

S

V

C

F

I

L

N

E

A

F

V

S

G

G

I

A

E

P

A

-

H

-

G

-

T

-

G

-

S

-

V

-

D

-

A

-

V

-

L

-

D

-

R

-

A

-

K

-

T

-

A

-

V

-

G

-

L

-

A

-

T

-

D

-

R

-

G

-

H

K

V

A

L

L

R

E

Q

Q

A

G

I

L

M

Q

A

A

C

V

R

T

K

P

G

A

G

G

T

R

I

V

S

S

I

L

P
x
L

G
|
G

V
x
L

Y

Y

A

P

G

G

V

K

L

V

-

T

-

I

-

D

-

F

-

N

D

N

K

L

V

I

P

I

F

F

G

K

A

A

A

L

F

T

A

I

K

Y

G

G

L
x
I

N
x
T

F

G

R
|
R

M

-

G

-

Q

-

T

-

H

H

M

L

M

W

R

E

Y

T

M

W

R

Y

P

T

L

V

L

S

E

R

R

L

I

L

E

Q

K

S

G

G

E

K

I

L

D

N

P

L

S

D

F

P

L

I

I

I

T

T

H

H

R

K

L

Y

S

K

-

G

L

F

E

D

D

K

G

Y

P

E

G

E

A

A

Y

F

R

E

T

L

F

M

R

R

D

A

K

G

Q

K

D

T

G

G

C

K

I

V

K

V

V

F

V

M

M

L

R

K

C42 (= C38) binding Zn(2+)
T44 (≠ S40) mutation to A: Total loss of enzymatic activity.
H67 (= H60) binding Zn(2+)
E68 (= E61) binding Zn(2+)
C97 (= C90) binding Zn(2+)
C100 (= C93) binding Zn(2+)
C103 (= C96) binding Zn(2+)
C111 (= C104) binding Zn(2+)
E152 (≠ F175) mutation E->A,Q: Almost complete loss of enzymatic activity.; mutation to C: 120-fold decrease in catalytic efficiency.; mutation to D: Shows 3-fold higher turnover rate and reduced affinities toward L-threonine and NAD(+), compared to wild-type.; mutation to K: Total loss of enzymatic activity.
L179 (≠ V201) binding NAD(+)
E199 (≠ D221) binding NAD(+); mutation to A: Large decrease in affinity for NAD(+).
R204 (= R226) binding NAD(+); mutation to A: Large decrease in affinity for NAD(+).
LGL 266:268 (≠ PGV 312:314) binding NAD(+)
IT 291:292 (≠ LN 332:333) binding NAD(+)
R294 (= R335) mutation to A: 4000-fold decrease in catalytic efficiency.

1y9aA Alcohol dehydrogenase from entamoeba histolotica in complex with cacodylate (see paper)
30% identity, 93% coverage: 25:392/395 of query aligns to 24:350/360 of 1y9aA

query
sites
1y9aA

P
|
P

G

L

D

D

I

A

I

L

V

V

K

R

V

P

T

L

S

A

T

L

A

A

I

P

C
|
C

G
x
T

S
|
S

D

D

L

T

H
|
H

-

T

L

V

Y

W

D

A

G

G

Y

A

I

I

P

G

T
x
D

M

-

R

R

Q
x
H

G

D

D

M

I

I

L

L

G

G

H
|
H

E
|
E

F

A

M

V

G

G

E

Q

V

I

V

V

E

K

T

V

G
|
G

R
x
S

E

L

V

V

K

K

T

R

L

L

K

K

V

V

G

G

D

D

R

K

V

V

I

I

V

V

P

P

F

A

T

I

I

-

A

-

C

-

G

-

C

-

F

-

C

-

N

-

R

-

Q

-

L

-

W

-

S

-

L

-

C

-

D

-

N

-

S

-

N

T

P

P

N
x
D

A

W

G

G

V
x
E

A

E

E

E

T
x
S

I

Q

F

R

G
|
G

-
x
Y

-

P

-
x
M

K

H

S
|
S

P

G

S

G

A

M

L

L

F

G

G

G

Y
x
W

S

K

F

F

L

-

M

-

G

S

G

N

F

F

A

K

G

D

G

G

Q

V

-

F

A

S

E

E

Y

V

V

F

R

H

V

V

P

N

Y

E

A

A

D

D

V

A

G

N

P

L

M

A

K

L

I

L

P

P

H

-

V

-

R

R

D

D

G

I

G

K

P

P

E

E

D

D

D

-

K

-

V

A

L

V

F

M

L

L

T

S

D
|
D

I

M

F

V

P

T

T
|
T

G

G

W

F

M
x
H

A

G

A

A

E

E

N

L

C

A

A

N

I

I

Q

K

D

L

G

G

D

D

V

T

V

V

A

C

V

V

W

I

G

G

C

I

G

G

P

P

V

V

G

G

Q

L

F

M

A

S

I

V

R

A

S

G

A

A

Q

N

L

H

Q

L

G

G

A

A

A

G

R

R

V

I

V

F

A

A

I

V

D

G

R

S

V

R

P

K

E

H

R

C

L

C

R

D

L

I

A

A

A

L

E

E

A

Y

G

G

A

A

-

T

E

D

T

I

I

I

D

N

F

Y

D

K

R

N

N

G

D

D

V

I

R

V

A

E

R

Q

L

I

D

L

E

K

L

A

T

T

G

D

G

G

R

K

G

G

P

V

D

D

S

K

C

V

I

V

N

I

A

A

V

G

G

G

I

-

E

D

A

V

H

H

G

-

T

-

G

-

S

-

V

-

D

-

A

-

V

T

L

F

D

A

R

Q

A

A

K

V

T

K

A

M

V

I

G

K

L

P

A

G

T

S

D

D

R

I

G

G

H

N

V

V

L

N

R

Y

Q

L

A

G

I

-

M

-

A

-

C

-

R

-

K

E

G

G

G

D

T

N

I

I

S

D

I

I

P

P

G

R

V

S

Y

E

A

W

G

G

V

V

L

-

D

-

K

-

V

-

P

-

F

-

G

-

A

-

F

-

A

-

K

-

G

-

L

-

N

-

F

-

R

G

M

M

G

G

Q

H

T

K

H

H

M

I

M

H

R

G

Y

G

M

L

R

T

P

P

-

G

-

R

-

V

-

R

-

M

-

E

-

K

L

L

L

A

E

S

R

L

I

I

E

S

K

T

G

G

E

K

I

L

D

D

P

T

S

S

F

K

L

L

I

I

T

T

H

H

R

R

L

F

S

E

-

G

L

L

E

E

D

K

G

V

P

E

G

D

A

A

Y

L

R

M

T

L

F

M

R

K

D

N

K

K

Q

P

D

A

G

D

C

L

I

I

K
|
K

V

P

V

V

M

V

R

R

active site: C37 (= C38), T38 (≠ G39), S39 (= S40), H42 (= H43), H59 (= H60), E60 (= E61), D89 (≠ N110), E92 (≠ V113), S95 (≠ T116), S103 (= S121), D150 (= D173), T154 (= T177), K346 (= K388)
binding acetate ion: P24 (= P25), G69 (= G70), S70 (≠ R71)
binding cacodylate ion: S39 (= S40), H59 (= H60), W110 (≠ Y128), D150 (= D173)
binding 1,2-ethanediol: G98 (= G119), Y99 (vs. gap), M101 (vs. gap), H157 (≠ M180)
binding zinc ion: C37 (= C38), D51 (≠ T51), H53 (≠ Q54), H59 (= H60), D150 (= D173)

3fplA Chimera of alcohol dehydrogenase by exchange of the cofactor binding domain res 153-295 of c. Beijerinckii adh by t. Brockii adh (see paper)
34% identity, 62% coverage: 22:266/395 of query aligns to 21:244/351 of 3fplA

query
sites
3fplA

I

V

A

A

E

G

P

S

G

Y

D

D

I

A

I

I

V

V

K

R

V

P

T

L

S

A

T

V

A

S

I

P

C
|
C

G
x
T

S
|
S

D

D

L

I

H
|
H

-

T

L

V

Y

F

D

E

G

G

Y

A

I

L

P

G

T

D

M

-

R

R

Q

K

G

N

D

M

I

I

L

L

G

G

H
|
H

E
|
E

F

A

M

V

G

G

E

E

V

V

E

E

T

V

G

G

R

S

E

E

V

V

K

K

T

D

L

F

K

K

V

P

G

G

D

D

R

R

V

V

I

I

V

V

P

P

F

-

T

-

I

-

A

-

C

-

G

-

C

-

F

-

C

C

N

T

R

T

Q

P

L
x
D

W

W

S

R

L
x
S

C

L

D

E

N

-

S

-

N

-

P
x
V

N

Q

A

A

G

G

V

F

A

Q

E

Q

T

-

I

-

F

-

G

-

K

H

S
|
S

P

N

S

G

A

M

L

L

F

A

G

G

Y

W

S

K

F

F

L

-

M

-

G

S

G

N

F

F

A

K

G

D

G

G

Q

V

-

F

A

G

E

E

Y

Y

V

F

R

H

V

V

P

N

Y

D

A

A

D

D

V

M

G

N

P

L

M

A

K

I

I

L

P

P

H

K

V

D

R

M

D

-

G

-

P

P

E

L

D

E

D

N

K

A

V

V

L

M

F

-

L

I

T

T

D
|
D

I

M

F

M

P

T

T
|
T

G

G

W

F

M

H

A

G

A

A

E

E

N

L

C

A

A

D

I

I

Q

E

D

L

G

G

D

A

V

T

V

V

A

A

V

V

W

L

G

G

C

I

G

G

P

P

V

V

G

G

Q

L

F

M

A

A

I

V

R

A

S

G

A

A

Q

K

L

L

Q

R

G

G

A

A

A

G

R

R

V

I

A

A

I

V

D

G

R

S

V

R

P

P

E

V

R

C

L

V

R

D

L

A

A

A

A

K

E

Y

A

Y

G

G

A

A

-

T

E

D

T

I

I

V

D

N

F

Y

D

K

R

D

N

G

D

P

V

I

R

E

A

S

R

Q

L

I

D

M

E

N

L

L

T

T

G

E

G

G

R

K

G

G

P

V

D

D

S

A

C

A

I

I

N

I

A

A

V

G

G

G

active site: C37 (= C38), T38 (≠ G39), S39 (= S40), H42 (= H43), H59 (= H60), E60 (= E61), D89 (≠ L100), S92 (≠ L103), V95 (≠ P109), S103 (= S121), D150 (= D173), T154 (= T177)
binding zinc ion: C37 (= C38), H59 (= H60), D150 (= D173)

Sites not aligning to the query:

active site: 346

P35630 NADP-dependent isopropanol dehydrogenase; EhADH1; EC 1.1.1.80 from Entamoeba histolytica (strain ATCC 30459 / HM-1:IMSS / ABRM) (see paper)
30% identity, 93% coverage: 25:392/395 of query aligns to 30:356/366 of P35630

query
sites
P35630

P

P

G

L

D

D

I

A

I

L

V

V

K

R

V

P

T

L

S

A

T

L

A

A

I

P

C
|
C

G

T

S

S

D

D

L

T

H

H

-

T

L

V

Y

W

D

A

G

G

Y

A

I

I

P

G

T

D

M

-

R

R

Q

H

G

D

D

M

I

I

L

L

G

G

H
|
H

E
|
E

F

A

M

V

G

G

E

Q

V

I

V

V

E

K

T

V

G

G

R

S

E

L

V

V

K

K

T

R

L

L

K

K

V

V

G

G

D

D

R

K

V

V

I

I

V

V

P

P

F

A

T

I

I

-

A

-

C

-

G

-

C

-

F

-

C

-

N

-

R

-

Q

-

L

-

W

-

S

-

L

-

C

-

D

-

N

-

S

-

N

T

P

P

N

D

A

W

G

G

V

E

A

E

E

E

T

S

I

Q

F

R

G

G

-

Y

-

P

-

M

K

H

S

S

P

G

S

G

A

M

L

L

F

G

G

G

Y

W

S

K

F

F

L

-

M

-

G

S

G

N

F

F

A

K

G

D

G

G

Q

V

-

F

A

S

E

E

Y

V

V

F

R

H

V

V

P

N

Y

E

A

A

D

D

V

A

G

N

P

L

M

A

K

L

I

L

P

P

H

-

V

-

R

R

D

D

G

I

G

K

P

P

E

E

D

D

D

-

K

-

V

A

L

V

F

M

L

L

T

S

D
|
D

I

M

F

V

P

T

T

T

G

G

W

F

M

H

A

G

A

A

E

E

N

L

C

A

A

N

I

I

Q

K

D

L

G

G

D

D

V

T

V

V

A

C

V

V

W

I

G

G

C

I

G

G

P

P

V

V

G

G

Q

L

F

M

A

S

I

V

R

A

S

G

A

A

Q

N

L

H

Q

L

G

G

A

A

A

G

R

R

V

I

V

F

A

A

I

V

D

G

R

S

V

R

P

K

E

H

R

C

L

C

R

D

L

I

A

A

A

L

E

E

A

Y

G

G

A

A

-

T

E

D

T

I

I

I

D

N

F

Y

D

K

R

N

N

G

D

D

V

I

R

V

A

E

R

Q

L

I

D

L

E

K

L

A

T

T

G

D

G

G

R

K

G

G

P

V

D

D

S

K

C

V

I

V

N

I

A

A

V

G

G

G

I

-

E

D

A

V

H

H

G

-

T

-

G

-

S

-

V

-

D

-

A

-

V

T

L

F

D

A

R

Q

A

A

K

V

T

K

A

M

V

I

G

K

L

P

A

G

T

S

D

D

R

I

G

G

H

N

V

V

L

N

R

Y

Q

L

A

G

I

-

M

-

A

-

C

-

R

-

K

E

G

G

G

D

T

N

I

I

S

D

I

I

P

P

G

R

V

S

Y

E

A

W

G

G

V

V

L

-

D

-

K

-

V

-

P

-

F

-

G

-

A

-

F

-

A

-

K

-

G

-

L

-

N

-

F

-

R

G

M

M

G

G

Q

H

T

K

H

H

M

I

M

H

R

G

Y

G

M

L

R

T

P

P

-

G

-

R

-

V

-

R

-

M

-

E

-

K

L

L

L

A

E

S

R

L

I

I

E

S

K

T

G

G

E

K

I

L

D

D

P

T

S

S

F

K

L

L

I

I

T

T

H

H

R

R

L

F

S

E

-

G

L

L

E

E

D

K

G

V

P

E

G

D

A

A

Y

L

R

M

T

L

F

M

R

K

D

N

K

K

Q

P

D

A

G

D

C

L

I

I

K

K

V

P

V

V

M

V

R

R

C43 (= C38) binding Zn(2+)
H65 (= H60) binding Zn(2+)
E66 (= E61) binding Zn(2+)
D156 (= D173) binding Zn(2+)

P81601 Alcohol dehydrogenase class-3 chain L; Alcohol dehydrogenase class-III chain L; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.284 from Gadus morhua (Atlantic cod) (see 2 papers)
29% identity, 93% coverage: 3:370/395 of query aligns to 12:354/375 of P81601

query
sites
P81601

A

A

L

V

C

A

W

W

H

E

G

A

K

G

E

K

D

P

V

L

R

S

V

M

E

E

R

E

V

V

P

-

D

-

P

-

R

E

I

V

A

A

E

P

P

P

-

Q

-

A

G

G

D

E

I

V

I

R

V

I

K

K

V

I

T

V

S

A

T

T

A

G

I

I

C

C

G

H

S

T

D

D

L

A

H

Y

L

T

Y

L

D

S

G

G

Y

S

I

D

P

P

T

E

M

G

R

V

Q

F

G

P

D

S

I

V

L

L

G

G

H

H

E

E

F

G

M

A

G

G

E

I

V

V

V

E

E

S

T

V

G

G

R

E

E

G

V

V

K

T

T

K

L

F

K

K

V

S

G

G

D

D

R

A

V

V

I

I

V

P

P

L

F

Y

T

V

I

P

A

Q

C

C

G

G

G

E

C

C

F

K

F

F

C

C

N

K

R

N

Q

P

L

K

W

T

S

N

L

L

C

C

D

Q

N

K

S

I

N

R

P

L

N

T

A

Q

G

G

V

K

A

G

E

L

T

M

I

P

F

N

G

G

K

T

S

S

P

R

S

F

A

S

L

C

F

N

G

G

Y

Q

S

V

F

L

L

F

M

H

G

-

F

F

A

M

G

G

G

S

Q

S

A

-

E

-

Y

-

V

T

R

F

V

S

P

E

Y

Y

A

T

D

V

V

V

G

A

P

E

M

I

K

S

I

L

P

A

H

K

V

V

R

H

D

E

G

K

G

A

P

P

E

L

D

-

D

D

K

K

V

V

L

C

F

L

L

L

T

G

D

C

I

A

F

I

P

S

T

T

G

G

W

Y

M

G

A

A

E

L

N

N

C

T

A

A

-

K

I

V

Q

E

D

A

G

G

D

S

V

T

V

C

A

A

V

V

W

F

G

G

C

L

G

G

P

A

V

L

G

G

Q

L

F

A

A

V

I

I

R

M

S

G

A

C

Q

Q

L

A

Q

A

G

G

A

A

A

S

R

R

V

I

A

A

I

I

D

D

R

V

V

N

P

P

E

D

R

K

L

F

R

R

L

I

A

A

A

K

E

E

A

F

G

G

A

A

E

T

T

D

I

L

D

V

F

N

D

P

R

K

N

D

D

H

-

S

-

K

-

P

V

V

R

E

A

Q

R

V

L

L

D

V

E

E

L

M

T

T

G

D

G

G

R

-

G

G

P

V

D

D

S

Y

C

S

I

F

N

E

A

C

V

V

G

G

I

N

E

I

A

A

H

-

G

-

T

-

G

-

S

-

V

-

D

-

A

-

V

-

L

-

D

-

R

-

A

-

K

-

T

-

A

-

V

-

G

-

L

-

A

-

T

-

D

-

R

-

G

-

H

-

V

V

L

M

R

R

Q

A

A

A

I

L

M

E

A

A

C

C

R

H

K

K

G

G

-

W

G

G

T

T

I

S

S

V

I

I

P

I

G

G

V

V

Y

A

A

A

G

A

-

G

-

Q

V

E

L

I

D

S

K

T

V

R

P

P

F

F

G

Q

A

L

A

V

F

T

A

G

-

R

-

T

-

W

K

K

G

G

L

T

N

A

F

F

R

-

M

-

G

G

Q

G

T

Y

H

K

M

S

M

V

R

E

Y

S

M

V

R

P

P

K

L

L

L

V

E

E

R

E

I

Y

E

M

K

N

G

K

E

K

I

V

D

K

P

V

S

D

F

E

L

F

I

V

T

T

H

H

R

T

L

L

S

P

L

F

E

E

Sites not aligning to the query:

1 modified: N-acetylalanine

5o9dA Crystal structure of r. Ruber adh-a, mutant y294f, w295a, y54f, f43h, h39y (see paper)
28% identity, 96% coverage: 1:381/395 of query aligns to 1:334/346 of 5o9dA

query
sites
5o9dA

M

M

K

K

A

A

L

V

C

Q

W

Y

H

T

-

E

-

I

G

G

K

S

E

E

D

P

V

V

R

V

V

V

E

D

R

-

V

I

P

P

D

T

P

P

R

T

I

-

A

P

E

G

P

P

G

G

D

E

I

I

I

L

V

L

K

K

V

V

T

T

S

A

T

A

A

G

I

L

C
|
C

G
x
Y

S
|
S

D

D

L

I

H
|
H

L

V

Y

M

D

D

G

-

Y

-

I

M

P

P

T

A

M

A

R

Q

Q

Y

G

A

-

F

-

G

-

L

-

P

D

L

I

T

L

L

G

G

H
|
H

E
|
E

F

G

M

V

G

G

E

T

V

V

V

A

E

E

T

L

G

G

R

E

E

G

V

V

K

T

T

G

L

F

K

G

V

V

G

G

D

D

R

A

V

V

I

A

V

V

P

Y

F

G

T

P

I

W

A

G

C
|
C

G

G

G

A

C
|
C

F

H

F

A

C
|
C

N

A

R

R

Q

G

L

R

W

E

S

N

L

Y

C
|
C

D

T

N

R

S

A

N
x
A

P

D

N

-

A

-

G

-

V

-

A

-

E

-

T

-

I

-

F

L

G

G

K

I

S

T

P

P

S

P

A

G

L

L

F

-

G

-

Y

-

S

-

F

-

L

-

M

-

G

-

G

G

F

S

A

P

G

G

G

S

Q

M

A

A

E

E

Y

Y

V

M

-

I

-

V

-

D

-

S

-

A

-

R

-

H

R

L

V

V

P

P

Y

I

A

G

D

D

V

L

G

D

P

P

M

V

K

A

I

A

P

A

H

P

V

L

R

T

D
|
D

G

A

G

G

P

-

E

-

D

-

K

-

V

-

L

-

F

-

L

-

T

-

D

-

I

L

F
x
T

P

P

T

Y

G

H

W

A

M

I

A

S

A

R

E

V

N

L

C

P

A

L

I

L

Q

G

D

P

G

G

D

S

V

T

V

A

A

V

V

V

W

I

G

G

C
x
V

G
|
G

P
x
G

V
x
L

G

G

Q

H

F

V

A

G

I

I

R

Q

S

I

A

L

Q

R

L

A

Q

V

G

S

A

A

R

R

V

V

V

I

A

A

I

V

D
|
D

R
x
L

V

D

P

D

E

D

R
|
R

L

L

R

A

L

L

A

A

A

R

E

E

A

V

G

G

A

A

E

D

T

A

I

A

D

V

F

K

D
x
S

R

G

N

A

D

G

V

A

R

A

A

D

R

A

L

I

D

R

E

E

L

L

T

T

G

G

R

Q

G

G

P

A

D

T

S

A

C

V

I

F

N

D

A
x
F

V
|
V

G

G

I

A

E

Q

A

-

H

-

G

-

T

-

G

-

S

-

V

-

D

-

A

S

V
x
T

L

I

D

D

R

T

A

A

K

Q

T

Q

A

V

V

V

G

-

L

-

A

A

T

V

D

D

R

-

G

-

H

-

V

-

L

-

R

-

Q

-

A

-

I

-

M

-

A

-

C

-

R

-

K

-

G

-

G

G

T

H

I

I

S

S

I

V

P
x
V

G
|
G

V
x
I

Y

H

A

A

G

G

V

A

L

H

D

A

K

K

V

V

-

G

-

F

-

M

-

I

P

P

F

F

G

G

A

A

A

S

F

V

A

V

K

T

G

-

L

-

N

P

F
|
F

R

A

M

G

G

T

Q

R

T

S

H

E

M

L

M

M

R

E

Y

-

M

-

R

-

P

-

L

V

E

A

R

L

I

A

E

R

K

A

G

G

E

R

I

L

D

D

P

-

S

-

F

-

L

I

I

H

T

T

H

E

R

T

L

F

S

T

L

L

E

D

D

E

G

G

P

P

G

A

A

A

Y

Y

R

R

T

R

F

L

R

R

D

E

active site: C38 (= C38), Y39 (≠ G39), S40 (= S40), H43 (= H43), H62 (= H60), E63 (= E61), C92 (= C90), C95 (= C93), C98 (= C96), C106 (= C104), A110 (≠ N108), D153 (= D160), T157 (≠ F175)
binding (2~{S})-2-methylpentanedioic acid: S40 (= S40), H62 (= H60), D153 (= D160), F294 (= F334)
binding nicotinamide-adenine-dinucleotide: C38 (= C38), Y39 (≠ G39), S40 (= S40), H43 (= H43), D153 (= D160), T157 (≠ F175), V180 (≠ C198), G181 (= G199), G182 (≠ P200), L183 (≠ V201), D203 (= D221), L204 (≠ R222), R208 (= R226), S223 (≠ D241), F246 (≠ A264), V247 (= V265), T252 (≠ V278), V269 (≠ P312), G270 (= G313), I271 (≠ V314), F294 (= F334)
binding zinc ion: C38 (= C38), H62 (= H60), C92 (= C90), C95 (= C93), C98 (= C96), C106 (= C104), D153 (= D160)

Sites not aligning to the query:

active site: 340
binding nicotinamide-adenine-dinucleotide: 340

P07913 L-threonine 3-dehydrogenase; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Escherichia coli (strain K12) (see paper)
28% identity, 99% coverage: 1:391/395 of query aligns to 1:338/341 of P07913

query
sites
P07913

M

M

K

K

A

A

L

L

C

S

-

K

W

L

H

K

G

A

K

E

E

E

D

G

V

I

R

W

V

M

E

T

R

D

V

V

P

P

D

V

P

P

R

E

I

L

A

G

E

H

P

-

G

N

D

D

I

L

V

I

K

K

V

I

T

R

S

K

T

T

A

A

I

I

C
|
C

G

G

S

T

D

D

L

V

H

H

L

I

Y

Y

D

N

G

W

-

D

-

E

-

W

Y

S

I

Q

P

K

T

T

M

I

R

P

Q

V

G

P

D

M

I

V

L

V

G

G

H

H

E

E

F

Y

M

V

G

G

E

E

V

V

E

G

T

I

G

G

R

Q

E

E

V

V

K

K

T

G

L

F

K

K

V

I

G

G

D

D

R

R

V

V

I

S

V

G

P

E

F

G

T

H

I

I

A

T

C

C

G

G

G

H

C

C

F

R

F

N

C

C

N

R

R

G

Q

G

L

R

W

T

S

H

L

L

C

C

D

R

N

N

S

T

N

-

P

I

N

G

A

V

G

G

V

V

A

N

E

R

T

-

I

-

F

-

G

-

K

-

S

-

P

-

S

-

A

-

L

-

F

-

G

-

Y

-

S

-

F

-

L

-

M

-

G

-

F

-

A

P

G

G

G

C

Q

F

A

A

E

E

Y

Y

V

L

R

V

V

I

P

P

Y

A

A

F

D

N

V

A

G

-

P

-

M

F

K

K

I

I

P

P

H

-

V

-

R

-

D

D

G

N

G

I

P

S

E

D

D

D

D

L

K

A

V

A

L

I

F

F

L

-

T

-

D

D

I

P

F

F

P

G

T

N

G

A

W

V

M

H

A

T

A

A

E

L

N

S

C

F

A

D

I

L

Q

V

D

G

G

E

D

D

V

V

V

L

A

-

V

V

W

S

G

G

C

A

G

G

P

P

V

I

G

G

Q

I

F

M

A

A

I

A

R

A

S

V

A

A

Q

K

L

H

Q

V

G

G

A

A

A

R

R

N

V

V

A

I

I

T

D

D

R

V

V

N

P

E

E

Y

R

R

L

L

R

E

L

L

A

A

A

R

E

K

A

M

G

G

-

I

A

T

E

R

T

A

I

V

D

N

F

V

D

A

R

K

N

E

D

N

V

L

R

N

A

D

R

V

L

M

D

A

E

E

L

L

T

G

G

M

G

T

R

E

G

G

P

F

D

D

S

-

C

-

I

-

N

-

A

-

V

V

G

G

I

L

E

E

A

M

H

S

G

G

T

A

G

P

S

P

V

-

D

-

A

-

V

-

L

-

D

-

R

-

A

-

K

-

T

-

A

-

V

-

G

-

L

-

A

-

T

-

D

-

R

-

G

-

H

-

V

A

L

F

R

R

Q

T

A

M

I

L

M

D

A

T

C

M

R

N

K

H

G

G

T

R

I

I

S

A

I

M

P

L

G

G

V

I

Y

P

A

P

G

S

V

D

L

M

D

S

-

I

-

D

-

W

-

T

K

K

V

V

P

I

F

F

G

K

A

G

A

L

F

F

A

I

K

K

G

G

L

I

N

Y

F

G

R

R

M

-

G

-

Q

-

T

-

H

E

M

M

M

F

R

E

Y

T

M

W

R

Y

P

K

L

M

L

A

E

A

R

L

I

I

E

Q

K

S

G

G

E

-

I

L

D

D

P

L

S

S

F

P

L

I

I

I

T

T

H

H

R

R

L

F

S

S

L

I

E

D

D

D

G

F

P

Q

G

K

A

G

Y

F

R

D

T

A

F

M

R

R

D

S

K

G

Q

Q

D

S

G

G

C

-

I

-

K

K

V

V

V

I

M

L

C38 (= C38) mutation to D: Shows only 1% of wild-type catalytic activity. This mutant can be stimulated to the wild-type activity level after incubation with Zn(+).; mutation to S: Loss of catalytic activity. This mutant cannot be stimulated to the wild-type activity level after incubation with Zn(+).

6schC Nadh-dependent variant of cbadh (see paper)
35% identity, 62% coverage: 22:266/395 of query aligns to 21:244/355 of 6schC

query
sites
6schC

I

V

A

A

E

G

P

S

G

Y

D

D

I

A

I

I

V

V

K

R

V

P

T

L

S

A

T

V

A

S

I

P

C
|
C

G
x
T

S
|
S

D

D

L

I

H
|
H

-

T

L

V

Y

F

D

E

G

G

Y

A

I

L

P

G

T

D

M

-

R

R

Q

K

G

N

D

M

I

I

L

L

G

G

H
|
H

E

E

F

A

M

V

G

G

E

E

V

V

E

E

T

V

G

G

R

S

E

E

V

V

K

K

T

D

L

F

K

K

V

P

G

G

D

D

R

R

V

V

I

I

V

V

P

P

F

-

T

-

I

-

A

-

C

-

G

-

C

-

F

-

C

C

N

T

R

T

Q

P

L

D

W

W

S

R

L

S

C

L

D

E

N

-

S

-

N

-

P

V

N

Q

A

A

G

G

V

F

A

Q

E

Q

T

-

I

-

F

-

G

-

K

H

S

S

P

N

S

G

A

M

L

L

F

A

G

G

Y
x
W

S

K

F

F

L

-

M

-

G

S

G

N

F

F

A

K

G

D

G

G

Q

V

-

F

A

G

E

E

Y

Y

V

F

R

H

V

V

P

N

Y

D

A

A

D

D

V

M

G

N

P

L

M

A

K

I

I

L

P

P

H

-

V

-

R

K

D

D

G

-

G

M

P

P

E

L

D

E

D

N

K

A

V

V

L

M

F

-

L

I

T

T

D
|
D

I

M

F

M

P

T

T
|
T

G

G

W

F

M

H

A

G

A

A

E

E

N

L

C

A

A

D

I

I

Q

Q

D

M

G

G

D

S

V

S

V

V

A

V

V

V

W

I

G
|
G

C

I

G

G

P

A

V
|
V

G

G

Q

L

F

M

A

G

I

I

R

A

S

G

A

A

Q

K

L

L

Q

R

G

G

A

A

A

G

R

R

V

I

A

G

I

V

D
|
D

R
x
Y

V
x
R

P

P

E

I

R

C

L

V

R

E

L

A

A

A

A

K

E

F

A

Y

G

G

A

A

E

T

T

D

I

I

D

L

F

N

D

P

R

K

N

N

-

G

D

H

V

I

R

V

A

D

R

Q

L

V

D

M

E

K

L

L

T

T

G

N

G

G

R

K

G

G

P

V

D

D

S

R

C

V

I

I

N

M

A
|
A

V
x
G

G
|
G

active site: C37 (= C38), S39 (= S40), H42 (= H43), H59 (= H60), D150 (= D173)
binding nicotinamide-adenine-dinucleotide: T38 (≠ G39), W110 (≠ Y128), D150 (= D173), T154 (= T177), G174 (= G197), V178 (= V201), D198 (= D221), Y199 (≠ R222), R200 (≠ V223), A242 (= A264), G243 (≠ V265), G244 (= G266)
binding zinc ion: C37 (= C38), H59 (= H60), D150 (= D173)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 266, 267, 340

6ie0B X-ray crystal structure of 2r,3r-butanediol dehydrogenase from bacillus subtilis
30% identity, 68% coverage: 1:267/395 of query aligns to 2:247/347 of 6ie0B

query
sites
6ie0B

M

M

K

K

A

A

L

A

C

R

W

W

H

H

G

N

K

Q

E

K

D

D

V

I

R

R

V

I

E

E

R

H

V

I

P

E

D

E

P

P

R

K

I

-

A

T

E

E

P

P

G

G

D

K

I

V

I

K

V

I

K

K

V

V

T

K

S

W

T

C

A

G

I

I

C
|
C

G

G

S
|
S

D

D

L

L

H
|
H

L

E

Y

Y

D

L

G

G

-

G

-

P

-

I

Y

F

I

I

P

P

T

V

M

D

R

K

Q

P

G

H

D

P

I

L

-

T

-

N

-

E

-

T

-

A

-

P

-

V

-

T

L

M

G

G

H
|
H

E

E

F

F

M

S

G

G

E

E

V

V

E

E

T

V

G

G

R

E

E

G

V

V

K

E

T

N

L

Y

K

K

V

V

G

G

D

D

R

R

V

V

I

V

V

V

P

E

F

P

T

I

I

F

A

A

C

T

-

H

-

G

-

H

G

Q

G

G

C

A

F

Y

F

N

C

L

N

D

R

E

Q

Q

L

M

W

G

S

F

L

L

C

G

D

L

N

A

S

G

-

G

-

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N

S

P

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N

Y

A

V

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V

A

D

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E

T

E

I

L

F

L

G

F

K

K

S

L

P

P

S

D

A

E

L

L

F

-

G

-

Y

-

S

S

F

Y

L

E

M

Q

G

G

G

A

F

L

A

V

G

E

G

P

Q

S

A

A

E

-

Y

-

V

-

R

-

V

-

P

-

Y

-

A

-

D

-

V

-

G

-

P

-

M

-

K

-

I

-

P

-

H

-

V

-

R

-

D

-

G

-

P

-

E

-

D

-

K

-

V

-

L

-

F

-

L

-

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-

D

-

I

-

F

-

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-

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V

G

A

W

L

M

Y

A

A

A

V

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R

N

S

C

S

A

K

I

L

Q

K

D

A

G

G

D

D

V

K

V

A

A

A

V

V

W

F

G

G

C

C

G

G

P

P

V

I

G

G

Q

L

F

L

A

V

I

I

R

E

S

A

A

L

Q

K

L

A

Q

A

G

G

A

A

A

T

R

D

V

I

V

Y

A

A

I

V

D

E

R

L

V

S

P

P

E

E

R

R

L

Q

R

Q

L

K

A

A

A

E

E

E

A

L

G

G

A

A

E

I

T

I

I

V

D

D

F

P

D

S

R

K

-

T

N

D

D

D

V

V

R

V

A

A

R

E

L

I

D

A

E

E

L

R

T

T

G

G

R

-

G

G

P

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C

A

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F

N

E

A

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T

G

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V

active site: C38 (= C38), S40 (= S40), H43 (= H43), H71 (= H60)
binding zinc ion: C38 (= C38), H71 (= H60)

8uasC Rhodococcus ruber alcohol dehydrogenase nadh biomimetic complex - compound 1a
28% identity, 96% coverage: 1:381/395 of query aligns to 4:337/348 of 8uasC

query
sites
8uasC

M

M

K

K

A

A

L

V

C

Q

W

Y

H

T

-

E

-

I

G

G

K

S

E

E

D

P

V

V

R

V

V

V

E

D

R

-

V

I

P

P

D

T

P

P

R

T

I

-

A

P

E

G

P

P

G

G

D

E

I

I

I

L

V

L

K

K

V

V

T

T

S

A

T

A

A

G

I

L

C
|
C

G

H

S
|
S

D

D

L

I

H

F

L

V

Y

M

D

D

G

-

Y

-

I

M

P

P

T

A

M

A

R

Q

Q

Y

G

A

D

Y

-

G

-

L

-

P

-

L

I

T

L

L

G

G

H
|
H

E
|
E

F

G

M

V

G

G

E

T

V

V

V

A

E

E

T

L

G

G

R

E

E

G

V

V

K

T

T

G

L

F

K

G

V

V

G

G

D

D

R

A

V

V

I

A

V

V

P

Y

F

G

T

P

I

W

A

G

C
|
C

G

G

G

A

C
|
C

F

H

F

A

C
|
C

N

A

R

R

Q

G

L

R

W

E

S

N

L

Y

C
|
C

D

T

N

R

S

A

N

A

P

D

N

-

A

-

G

-

V

-

A

-

E

-

T

-

I

-

F

L

G

G

K

I

S

T

P

P

S

P

A

G

L

L

F

-

G

-

Y

-

S

-

F

-

L

-

M

-

G

-

G

G

F

S

A

P

G

G

G

S

Q

M

A

A

E

E

Y

Y

V

M

-

I

-

V

-

D

-

S

-

A

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R

-

H

R

L

V

V

P

P

Y

I

A

G

D

D

V

L

G

D

P

P

M

V

K

A

I

A

P

A

H

P

V

L

R

T

D
|
D

G

A

G

G

P

-

E

-

D

-

K

-

V

-

L

-

F

-

L

-

T

-

D

-

I

L

F
x
T

P

P

T

Y

G

H

W

A

M

I

A

S

A

R

E

V

N

L

C

P

A

L

I

L

Q

G

D

P

G

G

D

S

V

T

V

A

A

V

V

V

W

I

G

G

C

V

G

G

P

G

V

L

G

G

Q

H

F

V

A

G

I

I

R

Q

S

I

A

L

Q

R

L

A

Q

V

G

S

A

A

R

R

V

V

V

I

A

A

I

V

D

D

R

L

V

D

P

D

E

D

R

R

L

L

R

A

L

L

A

A

A

R

E

E

A

V

G

G

A

A

E

D

T

A

I

A

D

V

F

K

D

S

R

G

N

A

D

G

V

A

R

A

A

D

R

A

L

I

D

R

E

E

L

L

T

T

G

G

R

Q

G

G

P

A

D

T

S

A

C

V

I

F

N

D

A

F

V

V

G

G

I

A

E

Q

A

-

H

-

G

-

T

-

G

-

S

-

V

-

D

-

A

S

V

T

L

I

D

D

R

T

A

A

K

Q

T

Q

A

V

V

V

G

-

L

-

A

A

T

V

D

D

R

-

G

-

H

-

V

-

L

-

R

-

Q

-

A

-

I

-

M

-

A

-

C

-

R

-

K

-

G

-

G

G

T

H

I

I

S

S

I

V

P
x
V

G

G

V

I

Y

H

A

A

G

G

V

A

L

H

D

A

K

K

V

V

-

G

-

F

-

M

-

I

P

P

F

F

G

G

A

A

A

S

F

V

A

V

K

T

G

-

L

-

N
x
P

F
x
Y

R
x
W

M

G

G

T

Q

R

T

S

H

E

M

L

M

M

R

E

Y

-

M

-

R

-

P

-

L

V

E

A

R

L

I

A

E

R

K

A

G

G

E

R

I

L

D

D

P

-

S

-

F

-

L

I

I

H

T

T

H

E

R

T

L

F

S

T

L

L

E

D

D

E

G

G

P

P

G

A

A

A

Y

Y

R

R

T

R

F

L

R

R

D

E

binding 1-[3-[~{tert}-butyl(dimethyl)silyl]oxypropyl]pyridine-3-carboxamide: S43 (= S40), D156 (= D160), T160 (≠ F175), V272 (≠ P312), P296 (≠ N333), Y297 (≠ F334), W298 (≠ R335)
binding zinc ion: C41 (= C38), H65 (= H60), E66 (= E61), C95 (= C90), C98 (= C93), C101 (= C96), C109 (= C104), D156 (= D160)

Query Sequence

>WP_012567805.1 NCBI__GCF_000016185.1:WP_012567805.1
MKALCWHGKEDVRVERVPDPRIAEPGDIIVKVTSTAICGSDLHLYDGYIPTMRQGDILGH
EFMGEVVETGREVKTLKVGDRVIVPFTIACGGCFFCNRQLWSLCDNSNPNAGVAETIFGK
SPSALFGYSFLMGGFAGGQAEYVRVPYADVGPMKIPHVRDGGPEDDKVLFLTDIFPTGWM
AAENCAIQDGDVVAVWGCGPVGQFAIRSAQLQGAARVVAIDRVPERLRLAAEAGAETIDF
DRNDVRARLDELTGGRGPDSCINAVGIEAHGTGSVDAVLDRAKTAVGLATDRGHVLRQAI
MACRKGGTISIPGVYAGVLDKVPFGAAFAKGLNFRMGQTHMMRYMRPLLERIEKGEIDPS
FLITHRLSLEDGPGAYRTFRDKQDGCIKVVMRPGG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory