SitesBLAST
Comparing WP_012568193.1 NCBI__GCF_000016185.1:WP_012568193.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4lmaA Crystal structure analysis of o-acetylserine sulfhydrylase cysk1 from microcystis aeruginosa 7806 (see paper)
64% identity, 93% coverage: 23:333/333 of query aligns to 2:312/318 of 4lmaA
4lmbA Crystal structure analysis of o-acetylserine sulfhydrylase cysk2 complexed with cystine from microcystis aeruginosa 7806 (see paper)
65% identity, 92% coverage: 24:329/333 of query aligns to 3:308/310 of 4lmbA
- active site: K46 (= K67), S269 (= S290)
- binding cysteine: K46 (= K67), T74 (= T95), S75 (= S96), N77 (= N98), T78 (= T99), M101 (= M122), M125 (= M146), M125 (= M146), Q147 (= Q168), F148 (= F169), Q224 (= Q245), G225 (= G246), G225 (= G246), I226 (= I247), A228 (= A249)
- binding pyridoxal-5'-phosphate: K46 (= K67), N77 (= N98), V180 (= V201), G181 (= G202), T182 (= T203), G183 (= G204), T185 (= T206), G225 (= G246), S269 (= S290), P296 (= P317)
2q3dA 2.2 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) from mycobacterium tuberculosis in complex with the reaction intermediate alpha-aminoacrylate (see paper)
63% identity, 92% coverage: 24:329/333 of query aligns to 3:305/306 of 2q3dA
- active site: K44 (= K67), S266 (= S290), P293 (= P317)
- binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: K44 (= K67), T71 (= T95), S72 (= S96), N74 (= N98), T75 (= T99), Q144 (= Q168), V177 (= V201), G178 (= G202), T179 (= T203), G180 (= G204), T182 (= T206), G222 (= G246), I223 (= I247), S266 (= S290), P293 (= P317), D294 (≠ S318)
P9WP55 O-acetylserine sulfhydrylase; OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase; EC 2.5.1.47 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
63% identity, 92% coverage: 24:329/333 of query aligns to 3:305/310 of P9WP55
- K44 (= K67) modified: N6-(pyridoxal phosphate)lysine
- N74 (= N98) binding pyridoxal 5'-phosphate
- GTGGT 178:182 (= GTGGT 202:206) binding pyridoxal 5'-phosphate
- S266 (= S290) binding pyridoxal 5'-phosphate
3zeiA Structure of the mycobacterium tuberculosis o-acetylserine sulfhydrylase (oass) cysk1 in complex with a small molecule inhibitor (see paper)
62% identity, 90% coverage: 24:324/333 of query aligns to 3:300/300 of 3zeiA
- active site: K44 (= K67), S266 (= S290), P293 (= P317)
- binding 3-[(Z)-[(5Z)-5-[[2-(2-hydroxy-2-oxoethyloxy)phenyl]methylidene]-3-methyl-4-oxidanylidene-1,3-thiazolidin-2-ylidene]amino]benzoic acid: T71 (= T95), S72 (= S96), I126 (= I150), Q144 (= Q168), F145 (= F169), K215 (≠ P239), G222 (= G246), A225 (= A249), F227 (= F251)
- binding pyridoxal-5'-phosphate: K44 (= K67), N74 (= N98), V177 (= V201), G178 (= G202), T179 (= T203), G180 (= G204), T182 (= T206), G222 (= G246), S266 (= S290), P293 (= P317), D294 (≠ S318)
2q3cA 2.1 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) holoenzyme from mycobacterium tuberculosis in complex with the inhibitory peptide dfsi (see paper)
62% identity, 90% coverage: 24:324/333 of query aligns to 3:300/300 of 2q3cA
- active site: K44 (= K67), S266 (= S290), P293 (= P317)
- binding : T71 (= T95), S72 (= S96), G73 (= G97), T75 (= T99), M122 (= M146), Q144 (= Q168), K215 (≠ P239), G222 (= G246), A225 (= A249)
4aecA Crystal structure of the arabidopsis thaliana o-acetyl-serine-(thiol)- lyasE C (see paper)
58% identity, 93% coverage: 23:332/333 of query aligns to 12:319/323 of 4aecA
- active site: K54 (= K67), S277 (= S290)
- binding pyridoxal-5'-phosphate: K54 (= K67), N85 (= N98), I188 (≠ V201), G189 (= G202), T190 (= T203), G191 (= G204), G192 (= G205), T193 (= T206), G233 (= G246), S277 (= S290), P304 (= P317)
P47999 Cysteine synthase, chloroplastic/chromoplastic; At.OAS.7-4; Beta-substituted Ala synthase 2;1; ARAth-Bsas2;1; CSase B; AtCS-B; CS-B; O-acetylserine (thiol)-lyase; O-acetylserine sulfhydrylase; OAS-TL B; cpACS1; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
58% identity, 93% coverage: 23:332/333 of query aligns to 74:381/392 of P47999
Sites not aligning to the query:
- 61 modified: N-acetylalanine
P47998 Cysteine synthase 1; At.OAS.5-8; Beta-substituted Ala synthase 1;1; ARAth-Bsas1;1; CSase A; AtCS-A; Cys-3A; O-acetylserine (thiol)-lyase 1; OAS-TL A; O-acetylserine sulfhydrylase; Protein ONSET OF LEAF DEATH 3; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
58% identity, 92% coverage: 23:330/333 of query aligns to 4:309/322 of P47998
- K46 (= K67) modified: N6-(pyridoxal phosphate)lysine; mutation to A: No cysteine synthase activity.
- T74 (= T95) mutation to A: Strong reduction of cysteine synthase activity.; mutation to S: Reduction of cysteine synthase activity.
- S75 (= S96) mutation S->A,N,T: Strong reduction of cysteine synthase activity.
- N77 (= N98) binding pyridoxal 5'-phosphate; mutation to A: Reduction of cysteine synthase activity.; mutation to D: Strong reduction of cysteine synthase activity.
- T78 (= T99) mutation T->A,S: Reduction of cysteine synthase activity.
- Q147 (= Q168) mutation Q->A,E: Strong reduction of cysteine synthase activity.
- H157 (= H178) mutation H->Q,N: Slight reduction of cysteine synthase activity.
- G162 (≠ A183) mutation to E: In old3-1; displays a early leaf death phenotype. Abolishes cysteine synthase activity.
- GTGGT 181:185 (= GTGGT 202:206) binding pyridoxal 5'-phosphate
- T182 (= T203) mutation T->A,S: Slight reduction of cysteine synthase activity.
- T185 (= T206) mutation T->A,S: Strong reduction of cysteine synthase activity.
- K217 (≠ L238) mutation to A: Impaired interaction with SAT1.
- H221 (= H242) mutation to A: Impaired interaction with SAT1.
- K222 (= K243) mutation to A: Impaired interaction with SAT1.
- S269 (= S290) binding pyridoxal 5'-phosphate; mutation to A: Strong reduction of cysteine synthase activity.; mutation to T: Reduction of cysteine synthase activity.
2isqA Crystal structure of o-acetylserine sulfhydrylase from arabidopsis thaliana in complex with c-terminal peptide from arabidopsis serine acetyltransferase (see paper)
58% identity, 92% coverage: 23:330/333 of query aligns to 2:307/320 of 2isqA
- active site: K44 (= K67), S267 (= S290)
- binding pyridoxal-5'-phosphate: K44 (= K67), N75 (= N98), G177 (= G200), G179 (= G202), T180 (= T203), G181 (= G204), T183 (= T206), G223 (= G246), S267 (= S290), P294 (= P317)
- binding : T72 (= T95), S73 (= S96), G74 (= G97), T76 (= T99), G122 (= G145), M123 (= M146), K124 (= K147), G217 (= G240), P218 (= P241), H219 (= H242), Q222 (= Q245), G223 (= G246)
1z7yA Crystal structure of the arabidopsis thaliana o-acetylserine sulfhydrylase k46a mutant (see paper)
58% identity, 92% coverage: 23:330/333 of query aligns to 2:307/320 of 1z7yA
- active site: A44 (≠ K67), S267 (= S290)
- binding n-[(3-hydroxy-2-methyl-5-{[(trihydroxyphosphoranyl)oxy]methyl}pyridin-4-yl)methylene]methionine: G74 (= G97), N75 (= N98), T76 (= T99), Q145 (= Q168), I178 (≠ V201), G179 (= G202), T180 (= T203), G181 (= G204), T183 (= T206), G223 (= G246), S267 (= S290), P294 (= P317), S295 (= S318)
7n2tA O-acetylserine sulfhydrylase from citrullus vulgaris in the internal aldimine state, with citrate bound (see paper)
58% identity, 92% coverage: 24:330/333 of query aligns to 3:307/309 of 7n2tA
5xoqA Crystal structure of o-acetylserine sulfhydrylase with bound transcription factor peptide inhibitor from planctomyces limnophilus
58% identity, 93% coverage: 24:332/333 of query aligns to 4:309/310 of 5xoqA
- binding : T72 (= T95), S73 (= S96), G74 (= G97), T76 (= T99), M123 (= M146), Q144 (= Q168), R218 (≠ P241), H219 (= H242), Q222 (= Q245), G223 (= G246), A226 (= A249)
6z4nAAA structure of oass complexed with upar inhibitor (see paper)
56% identity, 92% coverage: 23:329/333 of query aligns to 4:313/321 of 6z4nAAA
- binding pyridoxal-5'-phosphate: K43 (= K67), N73 (= N98), V177 (= V201), G178 (= G202), T179 (= T203), G180 (= G204), T182 (= T206), G230 (= G246), S274 (= S290), P301 (= P317)
- binding (1~{S},2~{S})-1-[(4-methylphenyl)methyl]-2-phenyl-cyclopropane-1-carboxylic acid: K43 (= K67), T70 (= T95), G72 (= G97), N73 (= N98), T74 (= T99), Q144 (= Q168), F145 (= F169), Q229 (= Q245), G230 (= G246), I231 (= I247), A233 (= A249)
P0ABK5 Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; S-carboxymethylcysteine synthase; Sulfate starvation-induced protein 5; SSI5; EC 2.5.1.47; EC 4.5.1.5 from Escherichia coli (strain K12) (see 5 papers)
56% identity, 93% coverage: 23:332/333 of query aligns to 3:315/323 of P0ABK5
- K42 (= K67) modified: N6-(pyridoxal phosphate)lysine; mutation to A: Still stimulates tRNase activity of CdiA-CT in vitro and in vivo.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
P0A1E3 Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; EC 2.5.1.47 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
56% identity, 92% coverage: 23:329/333 of query aligns to 3:312/323 of P0A1E3
- N72 (= N98) binding pyridoxal 5'-phosphate
- S273 (= S290) binding pyridoxal 5'-phosphate
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
3vbeC Crystal structure of beta-cyanoalanine synthase in soybean (see paper)
49% identity, 94% coverage: 20:332/333 of query aligns to 3:317/329 of 3vbeC
- active site: K52 (= K67), S81 (= S96), E212 (= E227), S216 (= S231), S275 (= S290), P302 (= P317)
- binding pyridoxal-5'-phosphate: K52 (= K67), N83 (= N98), M184 (≠ A199), G187 (= G202), S188 (≠ T203), G189 (= G204), T191 (= T206), G231 (= G246), S275 (= S290), P302 (= P317)
8b9wA Cysteine synthase from trypanosoma theileri with plp bound (see paper)
52% identity, 89% coverage: 32:329/333 of query aligns to 17:311/329 of 8b9wA
1d6sA Crystal structure of the k41a mutant of o-acetylserine sulfhydrylase complexed in external aldimine linkage with methionine (see paper)
56% identity, 92% coverage: 23:329/333 of query aligns to 2:311/322 of 1d6sA
- active site: A41 (≠ K67), G228 (= G246)
- binding methionine: T68 (= T95), N69 (≠ S96), N71 (= N98), T72 (= T99), Q142 (= Q168), F143 (= F169), G176 (= G202), G228 (= G246)
- binding pyridoxal-5'-phosphate: N71 (= N98), G176 (= G202), T177 (= T203), G178 (= G204), T180 (= T206), G228 (= G246), S272 (= S290), P299 (= P317)
3vc3A Crystal structure of beta-cyanoalanine synthase k95a mutant in soybean (see paper)
49% identity, 93% coverage: 23:332/333 of query aligns to 3:310/322 of 3vc3A
- active site: A45 (≠ K67), S268 (= S290), P295 (= P317)
- binding n-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-l-cysteine: T73 (= T95), S74 (= S96), N76 (= N98), M77 (≠ T99), Q146 (= Q168), M177 (≠ A199), G180 (= G202), S181 (≠ T203), G182 (= G204), T184 (= T206), G224 (= G246), S268 (= S290), P295 (= P317)
Query Sequence
>WP_012568193.1 NCBI__GCF_000016185.1:WP_012568193.1
MPALDAPTAHAPDSDGTAEFRGRIYDSILDTVGATPLVRLSRLAAKHGVVADVVAKCEFF
NPLSSVKDRIGKAMIEAAEADGLIAPDRTVLVEPTSGNTGIALAFVAAAKGYRLILTMPE
SMSVERRKMLKLLGAELVLTPAPNGMKGAIARAEEIVASTPDAYMLQQFKNPANPAVHRR
TTAEEIWRDTAGRADVLVAGVGTGGTLTGVAEVLKARRPDFRVVAVEPEDSPVLSGGLPG
PHKIQGIGAGFVPDVLARDRIDEVLRISNSRAFELARDAAATEGLAVGISSGAALAAAFE
VGARPEMKDRLIVVILPSFAERYLSTALFDGLD
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory