SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012568435.1 NCBI__GCF_000016185.1:WP_012568435.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P34047 Imidazoleglycerol-phosphate dehydratase 1, chloroplastic; IGPD 1; Protein HISTIDINE BIOSYNTHESIS 5A; EC 4.2.1.19 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
51% identity, 95% coverage: 11:208/209 of query aligns to 73:270/270 of P34047

query
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P34047

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H110 (= H48) binding Mn(2+)
H136 (= H74) binding Mn(2+)
H137 (= H75) binding Mn(2+)
E140 (= E78) binding Mn(2+)
H208 (≠ E146) binding Mn(2+)
H232 (= H170) binding Mn(2+)
H233 (= H171) binding Mn(2+)
E236 (= E174) binding Mn(2+)

O23346 Imidazoleglycerol-phosphate dehydratase 2, chloroplastic; IGPD 2; Protein HISTIDINE BIOSYNTHESIS 5B; EC 4.2.1.19 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
50% identity, 98% coverage: 4:208/209 of query aligns to 67:272/272 of O23346

query
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O23346

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E73 (vs. gap) mutation to Q: Loss of activity.
E86 (= E22) binding substrate
H112 (= H48) binding Mn(2+)
112:120 (vs. 48:56, 67% identical) binding substrate
H138 (= H74) binding Mn(2+)
HHTNE 138:142 (≠ HHTTE 74:78) binding substrate
H139 (= H75) binding Mn(2+)
E142 (= E78) binding Mn(2+)
R164 (= R100) binding substrate
R186 (= R122) binding substrate
H210 (≠ E146) binding Mn(2+)
H234 (= H170) binding Mn(2+)
234:242 (vs. 170:178, 56% identical) binding substrate
H235 (= H171) binding Mn(2+)
E238 (= E174) binding Mn(2+)
SSK 264:266 (≠ STK 200:202) binding substrate

5el9A A. Thaliana igpd2 in complex with the triazole-phosphonate inhibitor, (s)-c348, to 1.1a resolution (see paper)
51% identity, 95% coverage: 11:208/209 of query aligns to 2:199/199 of 5el9A

query
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5el9A

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binding [(2S)-2-hydroxy-3-(1H-1,2,4-triazol-1-yl)propyl]phosphonic acid: R91 (= R100), R113 (= R122), S191 (= S200), K193 (= K202)

6fwhL Acanthamoeba igpd in complex with r-c348 to 1.7a resolution (see paper)
53% identity, 93% coverage: 12:205/209 of query aligns to 2:194/195 of 6fwhL

query
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6fwhL

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binding [(2R)-2-hydroxy-3-(1H-1,2,4-triazol-1-yl)propyl]phosphonic acid: E12 (= E22), H38 (= H48), H64 (= H74), H65 (= H75), E68 (= E78), R90 (= R100), R112 (= R122), H160 (= H170), H161 (= H171), E164 (= E174), S189 (= S200), K191 (= K202)
binding magnesium ion: Q5 (≠ T15), Q80 (≠ K90)
binding manganese (ii) ion: H38 (= H48), H64 (= H74), E68 (= E78), H136 (≠ E146), H160 (= H170), E164 (= E174)

6fwhA Acanthamoeba igpd in complex with r-c348 to 1.7a resolution (see paper)
53% identity, 93% coverage: 12:205/209 of query aligns to 3:195/196 of 6fwhA

query
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6fwhA

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binding [(2R)-2-hydroxy-3-(1H-1,2,4-triazol-1-yl)propyl]phosphonic acid: E13 (= E22), H39 (= H48), H65 (= H74), H66 (= H75), E69 (= E78), R91 (= R100), R113 (= R122), H161 (= H170), H162 (= H171), E165 (= E174), S190 (= S200), K192 (= K202)
binding manganese (ii) ion: H39 (= H48), H65 (= H74), E69 (= E78), H137 (≠ E146), H161 (= H170), E165 (= E174)

5ekwA A. Thaliana igpd2 in complex with the racemate of the triazole- phosphonate inhibitor, c348 (see paper)
51% identity, 92% coverage: 11:203/209 of query aligns to 2:194/194 of 5ekwA

query
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5ekwA

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T

T

E

Q

L

L

F

V

K

E

E

H

W

F

F

F

Q

Q

A

S

F

L

A

V

Q

N

A

T

A

S

G

G

A

M

T

T

L

L

H

H

V

I

E

R

T

Q

L

L

Y

A

G

G

E

K

N

N

N

S

H

H

I

I

V

I

E

E

S

A

C

T

Y

F

K

K

A

A

L

F

A

A

R

R

A

A

L

L

R

R

A

Q

A

A

V

T

E

E

I

S

D

D

P

P

R

R

K

R

A

G

D

G

A

T

V

I

P

P

S
|
S

T

S

K
|
K

G

G

binding [(2S)-2-hydroxy-3-(1H-1,2,4-triazol-1-yl)propyl]phosphonic acid: R91 (= R100), R113 (= R122), S191 (= S200), K193 (= K202)
binding [(2R)-2-hydroxy-3-(1H-1,2,4-triazol-1-yl)propyl]phosphonic acid: R91 (= R100), R113 (= R122), S191 (= S200), K193 (= K202)

2f1dA X-ray structure of imidazoleglycerol-phosphate dehydratase (see paper)
51% identity, 88% coverage: 11:193/209 of query aligns to 1:183/183 of 2f1dA

query
sites
2f1dA

A

G

R

R

R

I

A

G

T

E

V

V

E

K

R

R

T

V

T

T

R

K

E

E

T

T

R

N

I

V

R

S

V

V

A

K

I

I

D

N

L

L

D

D

G

G

T

T

G

G

R

V

Y

A

A

D

V

S

S

S

T

S

G

G

I

I

G

P

F

F

L

L

D

D

H
|
H

M

M

L

L

E

D

Q

Q

L

L

S

A

R

S

H

H

S

G

L

L

I

F

D

D

L

V

E

H

V

V

A

R

A

A

E

T

G

G

D

D

L

V

H

H

I

I

D

D

F

D

H
|
H

H

H

T

T

T

N

E
|
E

D

D

T

I

G

A

I

L

A

A

I

I

G

G

E

T

A

A

V

L

A

L

K

K

A

A

L

L

G

G

E

E

R

R

R

K

G

G

I

I

Q

N

R

R

Y

F

G

G

H

D

A

F

Y

T

V

A

P

P

M

L

D

D

E

E

T

A

L

L

T

I

R

H

A

V

A

S

I

L

D

D

L

L

S

S

N

G

R

R

P

P

Y

Y

L

L

I

G

W

Y

K

N

V

L

A

E

F

I

S

P

R

T

D

Q

K

R

L

V

G

G

Q

T

M

Y

D

D

T

T

E

Q

L

L

F

V

K

E

E
x
H

W

F

F

F

Q

Q

A

S

F

L

A

V

Q

N

A

T

A

S

G

G

A

M

T

T

L

L

H

H

V

I

E

R

T

Q

L

L

Y

A

G

G

E

E

N

N

N

S

H
|
H

H

H

I

I

V

I

E
|
E

S

A

C

T

Y

F

K

K

A

A

L

F

A

A

R

R

A

A

L

L

R

R

A

Q

A

A

V

T

E

E

I

T

D

D

P

P

R

R

binding manganese (ii) ion: H38 (= H48), H64 (= H74), E68 (= E78), H136 (≠ E146), H160 (= H170), E164 (= E174)

4qnkA The structure of wt a. Thaliana igpd2 in complex with mn2+ and phosphate (see paper)
51% identity, 88% coverage: 11:194/209 of query aligns to 2:185/185 of 4qnkA

query
sites
4qnkA

A

A

R

R

R

I

A

G

T

E

V

V

E

K

R

R

T

E

T

T

R

K

E

E

T

T

R

N

I

V

R

S

V

V

A

K

I

I

D

N

L

L

D

D

G

G

T

H

G

G

R

V

Y

S

A

D

V

S

S

S

T

T

G

G

I

I

G

P

F

F

L

L

D

D

H
|
H

M

M

L

L

E

D

Q
|
Q

L

L

S

A

R

S

H
|
H

S

G

L

L

I

F

D

D

L

V

E

H

V

V

A

R

A

A

E

T

G

G

D

D

L

T

H

H

I

I

D

D

F

D

H
|
H

H

H

T

T

T

N

E
|
E

D

D

T

V

G

A

I

L

A

A

I

I

G

G

E

T

A

A

V

L

A

L

K

K

A

A

L

L

G

G

E

E

R

R

R

K

G

G

I

I

Q

N

R

R

Y

F

G

G

H

D

A

F

Y

T

V

A

P

P

M

L

D

D

E

E

T

A

L

L

T

I

R

H

A

V

A

S

I

L

D

D

L

L

S

S

N

G

R

R

P

P

Y

Y

L

L

I

G

W

Y

K

N

V

L

A

E

F

I

S

P

R

T

D

Q

K

R

L

V

G

G

Q

T

M

Y

D

D

T

T

E

Q

L

L

F

V

K

E

E
x
H

W

F

F

F

Q

Q

A

S

F

L

A

V

Q

N

A

T

A

S

G

G

A

M

T

T

L

L

H

H

V

I

E

R

T

Q

L

L

Y

A

G

G

E

K

N

N

N

S

H
|
H

H

H

I

I

V

I

E
|
E

S

A

C

T

Y

F

K
|
K

A

A

L

F

A

A

R

R

A

A

L

L

R

R

A

Q

A

A

V

T

E

E

I

S

D

D

P

P

R

R

K

R

binding manganese (ii) ion: H39 (= H48), H65 (= H74), E69 (= E78), H137 (≠ E146), H161 (= H170), E165 (= E174)
binding phosphate ion: Q43 (= Q52), H47 (= H56), K169 (= K178)

4mu1A The structure of wt a. Thaliana igpd2 in complex with mn2+, imidazole, and sulfate at 1.5 a resolution (see paper)
51% identity, 88% coverage: 11:194/209 of query aligns to 2:185/185 of 4mu1A

query
sites
4mu1A

A

A

R

R

R

I

A

G

T

E

V

V

E

K

R

R

T

E

T

T

R

K

E

E

T

T

R

N

I

V

R

S

V

V

A

K

I

I

D

N

L

L

D

D

G

G

T

H

G

G

R

V

Y

S

A

D

V

S

S

S

T

T

G

G

I

I

G

P

F

F

L

L

D

D

H

H

M

M

L

L

E

D

Q

Q

L

L

S

A

R

S

H

H

S

G

L

L

I

F

D

D

L

V

E

H

V

V

A

R

A

A

E

T

G

G

D

D

L

T

H

H

I

I

D

D

F

D

H
|
H

H

H

T

T

T

N

E
|
E

D

D

T

V

G

A

I

L

A

A

I

I

G

G

E

T

A

A

V

L

A

L

K

K

A

A

L

L

G

G

E

E

R

R

R

K

G

G

I

I

Q

N

R

R

Y

F

G

G

H

D

A

F

Y

T

V

A

P

P

M

L

D

D

E

E

T

A

L

L

T

I

R

H

A

V

A

S

I

L

D

D

L

L

S

S

N

G

R

R

P

P

Y

Y

L

L

I

G

W

Y

K

N

V

L

A

E

F

I

S

P

R

T

D

Q

K

R

L

V

G

G

Q

T

M

Y

D

D

T

T

E

Q

L

L

F

V

K

E

E
x
H

W

F

F

F

Q

Q

A

S

F

L

A

V

Q

N

A

T

A

S

G

G

A

M

T

T

L

L

H

H

V

I

E

R

T

Q

L

L

Y

A

G

G

E

K

N

N

N

S

H

H

I

I

V

I

E

E

S

A

C

T

Y

F

K

K

A

A

L

F

A

A

R

R

A

A

L

L

R

R

A

Q

A

A

V

T

E

E

I

S

D

D

P

P

R

R

K

R

binding imidazole: H65 (= H74), E69 (= E78)
binding manganese (ii) ion: H65 (= H74), E69 (= E78), H137 (≠ E146)

4mu0A The structure of wt a. Thaliana igpd2 in complex with mn2+ and 1,2,4- triazole at 1.3 a resolution (see paper)
51% identity, 88% coverage: 11:194/209 of query aligns to 2:185/185 of 4mu0A

query
sites
4mu0A

A

A

R

R

R

I

A

G

T

E

V

V

E

K

R

R

T

E

T

T

R

K

E

E

T

T

R

N

I

V

R

S

V

V

A

K

I

I

D

N

L

L

D

D

G

G

T

H

G

G

R

V

Y

S

A

D

V

S

S

S

T

T

G

G

I

I

G

P

F

F

L

L

D

D

H

H

M

M

L

L

E

D

Q

Q

L

L

S

A

R

S

H

H

S

G

L

L

I

F

D

D

L

V

E

H

V

V

A

R

A

A

E

T

G

G

D

D

L

T

H

H

I

I

D

D

F

D

H
|
H

T

T

T

N

E
|
E

D

D

T

V

G

A

I

L

A

A

I

I

G

G

E

T

A

A

V

L

A

L

K

K

A

A

L

L

G

G

E

E

R

R

R

K

G

G

I

I

Q

N

R

R

Y

F

G

G

H

D

A

F

Y

T

V

A

P

P

M

L

D

D

E

E

T

A

L

L

T

I

R

H

A

V

A

S

I

L

D

D

L

L

S

S

N

G

R

R

P

P

Y

Y

L

L

I

G

W

Y

K

N

V

L

A

E

F

I

S

P

R

T

D

Q

K

R

L

V

G

G

Q

T

M

Y

D

D

T

T

E

Q

L

L

F

V

K

E

E
x
H

W

F

F

F

Q

Q

A

S

F

L

A

V

Q

N

A

T

A

S

G

G

A

M

T

T

L

L

H

H

V

I

E

R

T

Q

L

L

Y

A

G

G

E

K

N

N

N

S

H

H

I

I

V

I

E

E

S

A

C

T

Y

F

K

K

A

A

L

F

A

A

R

R

A

A

L

L

R

R

A

Q

A

A

V

T

E

E

I

S

D

D

P

P

R

R

K

R

binding manganese (ii) ion: H65 (= H74), E69 (= E78), H137 (≠ E146)
binding 1,2,4-triazole: H65 (= H74), H66 (= H75)

4mu3A The form a structure of an e21q catalytic mutant of a. Thaliana igpd2 in complex with mn2+ and a mixture of its substrate, 2r3s-igp, and an inhibitor, 2s3s-igp, to 1.12 a resolution (see paper)
50% identity, 89% coverage: 11:195/209 of query aligns to 2:186/186 of 4mu3A

query
sites
4mu3A

A

A

R

R

R

I

A

G

T

E

V

V

E

K

R

R

T

E

T

T

R

K

E
x
Q

T

T

R

N

I

V

R

S

V

V

A

K

I

I

D

N

L

L

D

D

G

G

T

H

G

G

R

V

Y

S

A

D

V

S

S

S

T

T

G

G

I

I

G

P

F

F

L

L

D

D

H

H

M

M

L

L

E

D

Q

Q

L

L

S

A

R

S

H

H

S

G

L

L

I

F

D

D

L

V

E

H

V

V

A

R

A

A

E

T

G

G

D

D

L

T

H

H

I

I

D

D

F

D

H
|
H

T

T

T

N

E
|
E

D

D

T

V

G

A

I

L

A

A

I

I

G

G

E

T

A

A

V

L

A

L

K

K

A

A

L

L

G

G

E

E

R

R

R

K

G

G

I

I

Q

N

R

R

Y

F

G

G

H

D

A

F

Y

T

V

A

P

P

M

L

D

D

E

E

T

A

L

L

T

I

R

H

A

V

A

S

I

L

D

D

L

L

S

S

N

G

R

R

P

P

Y

Y

L

L

I

G

W

Y

K

N

V

L

A

E

F

I

S

P

R

T

D

Q

K

R

L

V

G

G

Q

T

M

Y

D

D

T

T

E

Q

L

L

F

V

K

E

E
x
H

W

F

F

F

Q

Q

A

S

F

L

A

V

Q

N

A

T

A

S

G

G

A

M

T

T

L

L

H

H

V

I

E

R

T

Q

L

L

Y

A

G

G

E

K

N

N

N

S

H

H

I

I

V

I

E

E

S

A

C

T

Y

F

K

K

A

A

L

F

A

A

R

R

A

A

L

L

R

R

A

Q

A

A

V

T

E

E

I

S

D

D

P

P

R

R

K

R

A

G

binding (2S,3S)-2,3-dihydroxy-3-(1H-imidazol-5-yl)propyl dihydrogen phosphate: H65 (= H74), H66 (= H75)
binding (2R,3S)-2,3-dihydroxy-3-(1H-imidazol-5-yl)propyl dihydrogen phosphate: Q13 (≠ E22), H65 (= H74), H66 (= H75)
binding manganese (ii) ion: H65 (= H74), E69 (= E78), H137 (≠ E146)

1rhyB Crystal structure of imidazole glycerol phosphate dehydratase (see paper)
51% identity, 85% coverage: 12:188/209 of query aligns to 3:177/180 of 1rhyB

query
sites
1rhyB

R

R

R

I

A

A

T

S

V

V

E

E

R

R

T

T

R

S

E

E

T

T

R

H

I

I

R

S

V

C

A

T

I

I

D

D

L

L

D

D

G

H

T

I

-

P

-

G

-

V

-

T

-

E

G

Q

R

K

Y

I

A

N

V

V

S

S

T

T

G

G

I

I

G

G

F

F

L

L

D

D

H

H

M

M

L

F

E

T

Q

A

L

L

S

A

R

K

H

H

S

G

L

G

I

M

D

S

L

L

E

Q

V

L

A

Q

A

C

E

K

G

G

D

D

L

-

H

-

I

-

D

-

F

-

H

-

T

T

T

A

E

E

D

D

T

C

G

A

I

L

A

A

I

L

G

G

E

E

A

A

V

F

A

K

K

K

A

A

L

L

G

G

E

E

R

R

R

K

G

G

I

I

Q

K

R

R

Y

Y

G

G

H

Y

A

A

Y

Y

V

A

P

P

M

L

D

D

E

E

T

S

L

L

T

S

R

R

A

A

A

V

I

I

D

D

L

I

S

S

N

S

R

R

P

P

Y

Y

L

F

I

M

W

C

K

H

V

L

A

P

F

F

S

T

R

R

D

E

K

K

L

V

G
|
G

Q

D

M

L

D

S

T

T

E

E

L

M

F

V

K

S

E

H

W

L

F

L

Q

Q

A

S

F

F

A

A

Q

F

A

A

G

G

A

V

T

T

L

L

H

H

V

I

E

D

T

S

L

I

Y

R

G

G

E

E

N

N

H
|
H

H

H

I

I

V

A

E

E

S

S

C

A

Y

F

K

K

A

A

L

L

A

A

R

L

A

A

L

I

R

R

A

M

A

A

V

I

binding glycerol: G126 (= G137), H159 (= H170)

8qavA Medicago truncatula hisn5 (igpd) in complex with mn and ig2 (see paper)
48% identity, 88% coverage: 11:194/209 of query aligns to 1:184/184 of 8qavA

query
sites
8qavA

A

A

R

R

R

I

A

G

T

E

V

M

E

K

R

R

T

V

T

T

R

K

E

E

T

T

R

N

I

V

R

S

V

V

A

K

I

I

D

N

L

L

D

D

G

G

T

T

G

G

R

V

Y

A

A

D

V

N

S

S

T

S

G

G

I

I

G

P

F

F

L

L

D

D

H
|
H

M

M

L

L

E

D

Q

Q

L

L

S

A

R

S

H

H

S

G

L

L

I

F

D

D

L

V

E

H

V

V

A

K

A

A

E

T

G

G

D

D

L

T

H

H

I

I

D

D

F

D

H
|
H

T

T

T

N

E
|
E

D

D

T

V

G

A

I

L

A

A

I

I

G

G

E

T

A

A

V

L

A

L

K

Q

A

A

L

L

G

G

E

D

R

R

R

K

G

G

I

I

Q

N

R

R

Y

F

G

G

H

N

A

F

Y

S

V

A

P

P

M

L

D

D

E

E

T

A

L

L

T

V

R

H

A

V

A

S

I

L

D

D

L

L

S

S

N

G

R

R

P

P

Y

H

L

L

I

G

W

Y

K

D

V

L

A

N

F

I

S

P

R

T

D

Q

K

R

L

V

G

G

Q

K

M

Y

D

D

T

T

E

Q

L

L

F

V

K

E

E
x
H

W

F

F

F

Q

Q

A

S

F

L

A

V

Q

N

A

T

A

S

G

G

A

M

T

T

L

L

H

H

V

I

E

R

T

Q

L

F

Y

S

G

G

E

T

N

N

N

S

H
|
H

H

H

I

I

V

I

E
|
E

S

A

C

T

Y

F

K

K

A

A

L

F

A

A

R

R

A

A

L

L

R

R

A

Q

A

A

V

T

E

E

I

Y

D

D

P

T

R

R

K

R

binding (2S,3S)-2,3-dihydroxy-3-(1H-imidazol-5-yl)propyl dihydrogen phosphate: H64 (= H74), H65 (= H75)
binding manganese (ii) ion: H38 (= H48), H64 (= H74), E68 (= E78), H136 (≠ E146), H160 (= H170), E164 (= E174)

8qawA Medicago truncatula hisn5 (igpd) in complex with mn, imd, edo, fmt, gol and trs (see paper)
48% identity, 88% coverage: 11:194/209 of query aligns to 2:185/185 of 8qawA

query
sites
8qawA

A

A

R

R

R

I

A

G

T

E

V

M

E

K

R

R

T

V

T

T

R

K

E
|
E

T

T

R

N

I

V

R

S

V

V

A

K

I

I

D

N

L

L

D

D

G

G

T

T

G

G

R

V

Y

A

A

D

V

N

S

S

T

S

G

G

I

I

G

P

F

F

L

L

D

D

H
|
H

M

M

L

L

E

D

Q
|
Q

L

L

S

A

R

S

H
|
H

S

G

L

L

I

F

D

D

L

V

E

H

V

V

A

K

A

A

E

T

G

G

D

D

L

T

H

H

I

I

D

D

F

D

H
|
H

T

T

T

N

E
|
E

D

D

T

V

G

A

I

L

A

A

I

I

G

G

E

T

A

A

V

L

A

L

K

Q

A

A

L

L

G

G

E

D

R

R

R

K

G

G

I

I

Q

N

R

R

Y

F

G

G

H

N

A

F

Y

S

V

A

P

P

M

L

D

D

E

E

T

A

L

L

T

V

R

H

A

V

A

S

I

L

D

D

L

L

S

S

N

G

R

R

P

P

Y

H

L

L

I

G

W

Y

K

D

V

L

A

N

F

I

S

P

R

T

D

Q

K

R

L

V

G

G

Q

K

M

Y

D

D

T

T

E

Q

L

L

F

V

K

E

E
x
H

W

F

F

F

Q

Q

A

S

F

L

A

V

Q

N

A

T

A

S

G

G

A

M

T

T

L

L

H

H

V

I

E

R

T

Q

L

F

Y

S

G

G

E

T

N

N

N

S

H
|
H

H

H

I

I

V

I

E
|
E

S

A

C

T

Y

F

K
|
K

A

A

L

F

A

A

R

R

A

A

L

L

R

R

A

Q

A

A

V

T

E

E

I

Y

D

D

P

T

R

R

K

R

binding glycerol: Q43 (= Q52), H47 (= H56), K169 (= K178)
binding imidazole: E13 (= E22), H66 (= H75), E69 (= E78)
binding manganese (ii) ion: H39 (= H48), H65 (= H74), E69 (= E78), H137 (≠ E146), H161 (= H170), E165 (= E174)

6ezmU Imidazoleglycerol-phosphate dehydratase from saccharomyces cerevisiae (see paper)
46% identity, 93% coverage: 12:205/209 of query aligns to 2:212/212 of 6ezmU

query
sites
6ezmU

R

Q

R

K

A

A

T

L

V

V

E

K

R

R

T

I

T

T

R

N

E

E

T

T

R

K

I

I

R

Q

V

I

A

A

I

I

D

S

L

L

D

K

G

G

-

G

-

P

-

L

-

A

-

I

-

E

-

H

-

S

-

I

-

F

-

P

-

E

-

K

-

A

-

E

-

Q

-

A

-

T

-

Q

T

S

G

Q

R

V

Y

I

A

N

V

V

S

H

T

T

G

G

I

I

G

G

F

F

L

L

D

D

H
|
H

M

M

L

I

E

H

Q

A

L

L

S

A

R

K

H

H

S

S

L

G

I

W

D

S

L

L

E

I

V

V

A

E

A

C

E

I

G

G

D

D

L

L

H

H

I

I

D

D

F

D

H
|
H

T

T

E
|
E

D

D

T

C

G

G

I

I

A

A

I

L

G

G

E

Q

A

A

V

F

A

K

K

E

A

A

L

L

G

G

E

A

R

V

R

R

G

G

I

V

Q

K

R

R

Y

F

G

G

H

S

A

G

Y

F

V

A

P

P

M

L

D

D

E

E

T

A

L

L

T

S

R

R

A

A

A

V

I

V

D

D

L

L

S

S

N

N

R

R

P

P

Y

Y

L

A

I

V

W

V

K

E

V

L

A

G

F

L

S

Q

R

R

D

E

K

K

L

V

G

G

Q

D

M

L

D

S

T

C

E

E

L

M

F

I

K

P

E
x
H

W

F

F

L

Q

E

A

S

F

F

A

A

Q

E

A

A

A

S

G

R

A

I

T

T

L

L

H

H

V

V

E

D

T

C

L

L

Y

R

G

G

E

K

N

N

N

D

H
|
H

I

R

V

S

E
|
E

S

S

C

A

Y

F

K
|
K

A

A

L

L

A

A

R

V

A

A

L

I

R

R

A

E

A

A

V

T

E

-

I

-

D

S

P

P

R

N

K

G

A

T

D

N

A

D

V

V

P

P

S
|
S

T

T

K
|
K

G

G

V

V

L

L

binding [(2R)-2-hydroxy-3-(1H-1,2,4-triazol-1-yl)propyl]phosphonic acid: H57 (= H48), H83 (= H74), H84 (= H75), E87 (= E78), H179 (= H170), H180 (= H171), E183 (= E174), K187 (= K178), S207 (= S200), K209 (= K202)
binding manganese (ii) ion: H57 (= H48), H83 (= H74), E87 (= E78), H155 (≠ E146), H179 (= H170), E183 (= E174)

P06633 Imidazoleglycerol-phosphate dehydratase; IGPD; EC 4.2.1.19 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
44% identity, 93% coverage: 12:205/209 of query aligns to 4:219/220 of P06633

query
sites
P06633

R

Q

R

K

A

A

T

L

V

V

E

K

R

R

T

I

T

T

R

N

E

E

T

T

R

K

I

I

R

Q

V

I

A

A

I

I

D

S

L

L

D

K

G

G

-

G

-

P

-

L

-

A

-

I

-

E

-

H

-

S

-

I

-

F

-

P

-

E

-

K

-

E

-

A

-

E

-

A

-

V

-

A

-

E

-

Q

-

A

-

T

-

Q

T

S

G

Q

R

V

Y

I

A

N

V

V

S

H

T

T

G

G

I

I

G

G

F

F

L

L

D

D

H
|
H

M
|
M

L
x
I

E
x
H

Q
x
A

L
|
L

S
x
A

R
x
K

H
|
H

S

S

L

G

I

W

D

S

L

L

E

I

V

V

A

E

A

C

E

I

G

G

D

D

L

L

H

H

I

I

D

D

F

D

H
|
H

T
|
T

E
|
E

D

D

T

C

G

G

I

I

A

A

I

L

G

G

E

Q

A

A

V

F

A

K

K

E

A

A

L

L

G

G

E

A

R

V

R

R

G

G

I

V

Q

K

R
|
R

Y

F

G

G

H

S

A

G

Y

F

V

A

P

P

M

L

D

D

E

E

T

A

L

L

T

S

R

R

A

A

A

V

I

V

D

D

L

L

S

S

N

N

R

R

P

P

Y

Y

L

A

I

V

W

V

K

E

V

L

A

G

F

L

S

Q

R

R

D

E

K

K

L

V

G

G

Q

D

M

L

D

S

T

C

E

E

L

M

F

I

K

P

E
x
H

W

F

F

L

Q

E

A

S

F

F

A

A

Q

E

A

A

A

S

G

R

A

I

T

T

L

L

H

H

V

V

E

D

T

C

L

L

Y

R

G

G

E

K

N

N

N

D

H
|
H

I
x
R

V
x
S

E
|
E

S
|
S

C
x
A

Y
x
F

K
|
K

A

A

L

L

A

A

R

V

A

A

L

I

R

R

A

E

A

A

V

T

E

-

I

-

D

S

P

P

R

N

K

G

A

T

D

N

A

D

V

V

P

P

S
|
S

T
|
T

K
|
K

G

G

V

V

L

L

H64 (= H48) binding Mn(2+)
64:72 (vs. 48:56, 44% identical) binding substrate
H90 (= H74) binding Mn(2+)
HHTTE 90:94 (= HHTTE 74:78) binding substrate
H91 (= H75) binding Mn(2+)
E94 (= E78) binding Mn(2+)
R116 (= R100) binding substrate
H162 (≠ E146) binding Mn(2+)
H186 (= H170) binding Mn(2+)
186:194 (vs. 170:178, 56% identical) binding substrate
H187 (= H171) binding Mn(2+)
E190 (= E174) binding Mn(2+)
STK 214:216 (= STK 200:202) binding substrate

Q9S5G5 Histidine biosynthesis bifunctional protein HisB; EC 3.1.3.15; EC 4.2.1.19 from Escherichia coli O157:H7 (see paper)
46% identity, 93% coverage: 12:205/209 of query aligns to 167:355/355 of Q9S5G5

query
sites
Q9S5G5

R

R

R

Y

A

A

T

H

V

V

E

V

R

R

T

N

T

T

R

K

E

E

T

T

R

Q

I

I

R

D

V

V

A

Q

I

V

D

W

L

L

D

D

G

R

T

E

G

G

R

G

Y

S

A

K

V

I

S

N

T

T

G

G

I

V

G

G

F

F

L

F

D

D

H

H

M

M

L

L

E

D

Q

Q

L

I

S

A

R

T

H

H

S

G

L

G

I

F

D

R

L

M

E

E

V

I

A

N

A

V

E

K

G

G

D

D

L

L

H

Y

I

I

D

D

F

D

H

H

T

T

T

V

E

E

D

D

T

T

G

G

I

L

A

A

I

L

G

G

E

E

A

A

V

L

A

K

K

I

A

A

L

L

G

G

E

D

R

K

R

R

G

G

I

I

Q

C

R

R

Y

F

G

G

H

F

A

V

Y

-

V

L

P

P

M

M

D

D

E

E

T

C

L

L

T

A

R

R

A

C

A

A

I

L

D

D

L

I

S

S

N

G

R

R

P

P

Y

H

L

L

I

E

W

Y

K

K

V

A

A

E

F

F

S

T

R

Y

D

Q

K

R

L

V

G

G

Q

D

M

L

D

S

T

T

E

E

L

M

F

I

K

E

E

H

W

F

F

F

Q

R

A

S

F

L

A

S

Q

Y

A

T

A

M

G

G

A

V

T

T

L

L

H

H

V

L

E

K

T

T

L

-

Y

K

G

G

E

K

N

N

N

D

H

H

I

R

V

V

E

E

S

S

C

L

Y

F

K

K

A

A

L

F

A

G

R

R

A

T

L

L

R

R

A

Q

A

A

V

I

E

R

I

V

D

E

P

-

R

-

K

-

A

G

D

D

A

T

V

L

P

P

S

S

T

S

K

K

G

G

V

V

L

L

Sites not aligning to the query:

1:166 Histidinol-phosphatase
9 binding Mg(2+)
11 binding Mg(2+)
17 E→A: Severe decrease in histidinol-phosphate phosphatase activity in presence of low magnesium concentration. At higher magnesium concentration (5mM), no effect on activity.
93 binding Zn(2+)
95 binding Zn(2+)
101 binding Zn(2+)
103 binding Zn(2+)
130 binding Mg(2+)

P40374 Imidazoleglycerol-phosphate dehydratase; IGPD; EC 4.2.1.19 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
46% identity, 93% coverage: 12:205/209 of query aligns to 2:216/216 of P40374

query
sites
P40374

R

R

A

A

T

F

V

V

E

E

R

R

T

N

T

T

R

N

E

E

T

T

R

K

I

I

R

S

V

V

A

A

I

I

D

A

L

L

D

D

G

K

-

A

-

P

-

L

-

P

-

E

-

S

-

N

-

F

-

I

-

D

-

E

-

L

-

I

T

T

G

S

R

K

Y

H

A

A

-

N

-

Q

-

K

-

G

-

E

-

Q

-

V

-

I

-

Q

V

V

S

D

T

T

G

G

I

I

G

G

F

F

L

L

D

D

H

H

M

M

L

Y

E

H

Q

A

L

L

S

A

R

K

H

H

S

A

L

G

I

W

D

S

L

L

E

R

V

L

A

Y

A

S

E

R

G

G

D

D

L

L

H

I

I

I

D

D

F

D

H

H

T

T

T

A

E

E

D

D

T

T

G

A

I

I

A

A

I

L

G

G

E

I

A

A

V

F

A

K

K

Q

A

A

L

M

G

G

E

N

R

F

R

A

G

G

I

V

Q

K

R

R

Y

F

G

G

H

H

A

A

Y

Y

V

C

P

P

M

L

D

D

E

E

T

A

L

L

T

S

R

R

A

S

A

V

I

V

D

D

L

L

S

S

N

G

R

R

P

P

Y

Y

L

A

I

V

W

I

K

D

V

L

A

G

F

L

S

K

R

R

D

E

K

K

L

V

G

G

Q

E

M

L

D

S

T

C

E

E

L

M

F

I

K

P

E

H

W

L

F

L

Q

Y

A

S

F

F

A

S

Q

V

A

A

G

G

A

I

T

T

L

L

H

H

V

V

E

T

T

C

L

L

Y

Y

G

G

E

S

N

N

N

D

H

H

I

R

V

A

E

E

S

S

C

A

Y

F

K

K

A

S

L

L

A

A

R

V

A

A

L

M

R

R

A

A

V

T

E

S

I

L

D

-

P

-

R

T

K

G

A

S

D

S

A

E

V

V

P

P

S
|
S

T

T

K

K

G

G

V

V

L

L

S211 (= S200) modified: Phosphoserine

P9WML9 Imidazoleglycerol-phosphate dehydratase; IGPD; EC 4.2.1.19 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
50% identity, 88% coverage: 22:205/209 of query aligns to 21:210/210 of P9WML9

query
sites
P9WML9

E

E

T

S

R

D

I

I

R

V

V

I

A

E

I

L

D

D

L

L

D

D

G

G

T

T

G

G

R

Q

Y

V

A

A

V

V

S

D

T

T

G

G

I

V

G

P

F

F

L

Y

D

D

H
|
H

M
|
M

L
|
L

E
x
T

Q
x
A

L
|
L

S
x
G

R
x
S

H
|
H

S

A

L

S

I

F

D

D

L

L

E

T

V

V

A

R

A

A

E

T

G

G

D

D

L

V

H

E

I

I

D

E

F

A

H
|
H

T
|
T

T
x
I

E
|
E

D

D

T

T

G

A

I

I

A

A

I

L

G

G

E

T

A

A

V

L

A

G

K

Q

A

A

L

L

G

G

E

D

R

K

R

R

G

G

I

I

Q

R

R

R

Y

F

G

G

H

D

A

A

Y

F

V

I

P

P

M

M

D

D

E

E

T

T

L

L

T

A

R

H

A

A

I

V

D

D

L

L

S

S

N

G

R

R

P

P

Y

Y

L

C

I

V

W

H

K

T

V

-

A

-

F

G

S

E

R

P

D

D

K

H

L

L

G

Q

Q

H

M

T

D

T

-

I

-

A

-

G

-

S

-

V

-

P

-

Y

-

H

T

T

E

V

L

I

F

N

K

R

E
x
H

W

V

F

F

Q

E

A

S

F

L

A

A

Q

A

A

N

A

A

G

R

A

I

T

A

L

L

H

H

V

V

E

R

T

V

L

L

Y

Y

G

G

E

R

N

D

N

P

H
|
H

I
|
I

V
x
T

E
|
E

S
x
A

C
x
Q

Y
|
Y

K
|
K

A

A

L

V

A

A

R

R

A

A

L

L

R

R

A

Q

A

A

V

V

E

E

I

P

D

D

P

P

R

R

K

-

A

V

D

S

A

G

V

V

P

P

S

S

T

T

K

K

G

G

V

A

L

L

H47 (= H48) binding Mn(2+)
47:55 (vs. 48:56, 56% identical) binding substrate
H73 (= H74) binding Mn(2+)
HHTIE 73:77 (≠ HHTTE 74:78) binding substrate
H74 (= H75) binding Mn(2+)
E77 (= E78) binding Mn(2+)
H152 (≠ E146) binding Mn(2+)
H176 (= H170) binding Mn(2+)
176:184 (vs. 170:178, 67% identical) binding substrate
H177 (= H171) binding Mn(2+)
E180 (= E174) binding Mn(2+)

5xdsA Crystal structure of mycobacterium tuberculosis hisb bound with an inhibitor (see paper)
49% identity, 82% coverage: 22:193/209 of query aligns to 12:190/191 of 5xdsA

query
sites
5xdsA

E

E

T

S

R

D

I

I

R

V

V

I

A

E

I

L

D

D

L

L

D

D

G

G

T

T

G

G

R

Q

Y

V

A

A

V

V

S

D

T

T

G

G

I

V

G

P

F

F

L

Y

D

D

H
|
H

M

M

L

L

E

T

Q

A

L

L

S

G

R

S

H

H

S

A

L

S

I

F

D

D

L

L

E

T

V

V

A

R

A

A

E

T

G

G

D

D

L

V

H

E

I

I

D

E

F

A

H
|
H

T

T

T

I

E
|
E

D

D

T

T

G

A

I

I

A

A

I

L

G

G

E

T

A

A

V

L

A

G

K

Q

A

A

L

L

G

G

E

D

R

K

R

R

G

G

I

I

Q

R

R

R

Y

F

G

G

H

D

A

A

Y

F

V

I

P

P

M

M

D

D

E

E

T

T

L

L

T

A

R

H

A

A

I

V

D

D

L

L

S

S

N

G

R

R

P

P

Y

Y

L

C

I

V

W

H

K

T

V

-

A

-

F

G

S

E

R

P

D

D

K

H

L

L

G

Q

Q

H

-

T

-

I

-

A

-

G

-

S

-

V

-

P

M

Y

D

H

T

T

E

V

L

I

F

N

K

R

E
x
H

W

V

F

F

Q

E

A

S

F

L

A

A

Q

A

A

N

A

A

G

R

A

I

T

A

L

L

H

H

V

V

E

R

T

V

L

L

Y

Y

G

G

E

R

N

D

N

P

H
|
H

H

H

I

I

V

T

E
|
E

S

A

C

Q

Y

Y

K

K

A

A

L

V

A

A

R

R

A

A

L

L

R

R

A

Q

A

A

V

V

E

E

I

P

D

D

P

P

R

R

binding (2S)-2-azanyl-3-(4H-1,2,4-triazol-3-yl)propanoic acid: H64 (= H74), H65 (= H75), E68 (= E78)
binding manganese (ii) ion: H38 (= H48), H64 (= H74), E68 (= E78), H143 (≠ E146), H167 (= H170), E171 (= E174)

Query Sequence

>WP_012568435.1 NCBI__GCF_000016185.1:WP_012568435.1
MDSSTPAPIPARRATVERTTRETRIRVAIDLDGTGRYAVSTGIGFLDHMLEQLSRHSLID
LEVAAEGDLHIDFHHTTEDTGIAIGEAVAKALGERRGIQRYGHAYVPMDETLTRAAIDLS
NRPYLIWKVAFSRDKLGQMDTELFKEWFQAFAQAAGATLHVETLYGENNHHIVESCYKAL
ARALRAAVEIDPRKADAVPSTKGVLGGSL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory