SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012569196.1 NCBI__GCF_000016185.1:WP_012569196.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

3nvtA 1.95 angstrom crystal structure of a bifunctional 3-deoxy-7- phosphoheptulonate synthase/chorismate mutase (aroa) from listeria monocytogenes egd-e (see paper)
32% identity, 27% coverage: 10:87/285 of query aligns to 1:74/345 of 3nvtA

query
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3nvtA

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Sites not aligning to the query:

binding manganese (ii) ion: 114, 284, 310, 321

5j6fA Crystal structure of dah7ps-cm complex from geobacillus sp. With prephenate (see paper)
32% identity, 29% coverage: 5:87/285 of query aligns to 1:84/352 of 5j6fA

query
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5j6fA

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binding prephenic acid: R25 (= R29), I32 (= I36), K36 (= K40), Y44 (≠ L47), D45 (≠ R48), R48 (= R51), M52 (≠ I55)

Sites not aligning to the query:

binding manganese (ii) ion: 124, 294, 320, 331

3tfcA 1.95 angstrom crystal structure of a bifunctional 3-deoxy-7- phosphoheptulonate synthase/chorismate mutase (aroa) from listeria monocytogenes egd-e in complex with phosphoenolpyruvate (see paper)
30% identity, 27% coverage: 11:87/285 of query aligns to 1:73/343 of 3tfcA

query
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3tfcA

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Sites not aligning to the query:

binding manganese (ii) ion: 113, 283, 309, 320
binding phosphoenolpyruvate: 137, 193, 196, 218, 248, 283

Query Sequence

>WP_012569196.1 NCBI__GCF_000016185.1:WP_012569196.1
MVPVSQTLNELRSEIDEIDAALHDLVMRRTALVEQIGAVKPPGRPALRPAREAEILRNLM
DRHQGAFPPAALSRMWREMISALTRLQGPFAVAVYAPDDQRGFWDLARDHYGSATPMQAA
NSSLAVLRLVAEGSATVGVLPWPEDDDPDPWWRYLFTADAKSPRIIARLPFLARAGREEV
EAMAIAALPLEETGDDRTLLGIELREDMSRGRLKDSLEAAGLPPLQLRTFHLPGGAGSVH
LTEVEAFVGPDDPRLAALYQRLGEALVRLLPIGTYATPIGLARRG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory