SitesBLAST

P30825 High affinity cationic amino acid transporter 1; CAT-1; CAT1; Ecotropic retroviral leukemia receptor homolog; Ecotropic retrovirus receptor homolog; Solute carrier family 7 member 1; System Y+ basic amino acid transporter from Homo sapiens (Human) (see paper)
37% identity, 83% coverage: 25:432/494 of query aligns to 24:436/629 of P30825

query
sites
P30825

E

E

T

E

P

T

N

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L

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C

L

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S

N

L

T

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A

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Y

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H

A

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N

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P

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F

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Y

Y

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Q

N226 (= N231) modified: carbohydrate, N-linked (GlcNAc...) asparagine

P76037 Putrescine importer PuuP from Escherichia coli (strain K12) (see paper)
26% identity, 94% coverage: 28:489/494 of query aligns to 17:448/461 of P76037

query
sites
P76037

N

R

L

L

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R

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K

T

S

L

L

S

K

L

L

A

W

S

Q

L

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M

L

M

G

G

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Y

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G

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I

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A

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A

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Y

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A

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G

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P

-

A

H

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L

A

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Y

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L

A

A

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L

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A

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Y

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A

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A

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Y
|
Y

A

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F

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A

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F

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G

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A

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W

W

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A

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N

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Y110 (= Y122) mutation to X: The uptake activity is reduced to one-eighth of that of wild-type.

Q7YQK4 Large neutral amino acids transporter small subunit 1; 4F2 light chain; 4F2 LC; 4F2LC; L-type amino acid transporter 1; LAT1; Solute carrier family 7 member 5 from Oryctolagus cuniculus (Rabbit) (see 2 papers)
26% identity, 95% coverage: 12:480/494 of query aligns to 25:469/503 of Q7YQK4

query
sites
Q7YQK4

S

K

L

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A

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A

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C

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Y

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V

M

L

M

F

W

L

L

R

R

Y

Y

T

K

H

K

P

P

Q

E

I

L

H

E

R

R

P

P

F

I

R

K

A

V

-

N

-

L

-

A

-

L

P

P

L

V

I

F

A

F

I

I

V

L

A

A

P
x
C

L

L

G

F

A

L

A

I

A

A

A

-

V

-

V

V

L

S

M

F

L

W

G

K

L

T

P

P

R

V

D

E

T
x
C

W

G

I

I

R

G

F

F

A

T

I

I

W

I

L

L

A

S

I

-

G

G

L

L

I

P

L

V

Y

Y

F

F

C88 (≠ A74) mutation to S: No significant effect on inhibition by HgCl(2). Decreased KM and Vmax for Phe. Similar affect on KM and Vmax for Phe; when associated with S-183.
C98 (= C84) mutation to S: No significant effect on inhibition by HgCl(2). Slightly decreased KM and Vmax for Phe. Slightly less decreased KM and Vmax for Phe; when associated with S-183.
C160 (≠ W166) mutation to S: No change to KM or Vmax for Phe.
C172 (≠ V178) mutation to S: No change to KM or Vmax for Phe.
C174 (≠ I180) mutation to S: No change to KM or Vmax for Phe.
C183 (≠ V189) mutation to S: No significant effect on inhibition by HgCl(2). Slightly decreased KM and Vmax for Phe. Similar affect on KM and Vmax for Phe; when associated with S-88. Slightly less decreased KM and Vmax for Phe; when associated with S-98.
G219 (≠ N225) mutation to D: Decreased KM and Vmax for Trp. Increased KM and Vmax for Phe; when associated with L-234.
W234 (≠ A241) mutation to L: Decreased KM and Vmax for Trp. Increased KM but decreased Vmax for Phe. Increased KM and Vmax for Phe; when associated with D-219.
C331 (≠ L343) mutation to S: No significant effect on inhibition by HgCl(2). Increased KM and Vmax for Phe.
C377 (≠ G389) mutation to S: No significant effect on inhibition by HgCl(2).
C403 (≠ A415) mutation to S: No significant effect on inhibition by HgCl(2).
C439 (≠ P447) mutation to S: Prevents insertion into the plasma membrane and possibly protein folding.
C454 (≠ T464) mutation to S: No significant effect on inhibition by HgCl(2). Slightly increased KM but slightly decreased Vmax for Phe.

Sites not aligning to the query:

492 C→S: No significant effect on inhibition by HgCl(2). Slightly decreased KM and Vmax for Phe.

8xxiA Structure of y+lat1 bound with leu
24% identity, 94% coverage: 25:488/494 of query aligns to 1:440/465 of 8xxiA

query
sites
8xxiA

E

E

T

Q

P

V

N

K

L

L

R

K

T

E

L

I

S

S

L

L

A

L

S

N

L

G

I

V

S

C

L

L

G

I

I

V

G

G

C
x
N

I
x
M

I
|
I

G
|
G

A
x
S

G
|
G

I

I

F

F

V

V

L

S

T

P

G

K

H

G

A

V

A

L

A

I

A

Y

Y

S

A

A

G

S

P

F

A

G

I

L

S

S

L

L

S

V

-

I

F

W

V

A

L

V

A

G

G

G

L

L

V

F

C

S

A

V

L

F

A

G

G

A

L

L

C

C

Y

Y

A

A

E

E

M

L

A

G

S

T

T

T

V

I

P

K

V

K

A

S

G

G

S

A

A

S

Y

Y

T

A

Y

Y

A

I

Y

L

A

E

T

A

L

F

G

G

E

G

F

F

I

L

A

A

W

F

I

I

I

R

G

L

W

W

-

T

D

S

L

L

L

I

E

I

Y

E

A

P

F

T

G

S

A

Q

T

A

T

I

V

I

A

A

I

I

G

T

W

F

S

A

G

N

Y

Y

V

M

V

V

S

Q

F

P

L

L

R

-

D

-

F

-

H

-

I

-

G

-

I

-

P

-

A

-

L

-

A

-

G

-

A

F

P

P

F

S

A

C

F

F

D

A

P

P

A

Y

S

A

G

A

A

S

W

-

T

-

H

-

T

-

G

-

A

-

L

-

V

-

N

R

A

L

P

L

A

A

V

A

A

A

I

C

V

I

L

C

A

L

L

L

T

T

A

F

L

I

L

N

V

C

V

A

G

Y

V

V

N

K

E

W

S

G

A

T

K

L

V

V

N

Q

N

D

I

I

I

F

V

T

A

Y

I

A

K

K

L

V

A

L

I

A

I

L

V

I

V

A

F

V

I

I

L

V

A

A

G

G

L

I

S

V

S

R

V

L

S

G

T

Q

A

G

N

-

W

-

V

-

T

A

S

S

A

T

N

H

P

F

D

E

G

N

A

S

F

F

I

E

P

G

P

S

N

S

A

F

G

A

P

V

G

G

E

D

Y

-

G

-

W

-

S

-

G

-

I

I

L

A

R

L

G

A

A

L

A

Y

V

S

V

A

F

L

F

F

A
x
S

Y

Y

I
x
S

G
|
G

F

W

D

D

A

T

V

L

S

N

T

Y

A

V

A

T

Q

E

E

E

A

I

K

K

N

N

P

P

Q

E

R

R

D

N

M

L

P

P

L

L

G

S

I

I

L

G

G

I

S

S

L

M

V

P

I

I

C

V

T

T

V

I

L

I

Y

Y

V

I

L

L

V

T

S

N

I

V

V

A

I

Y

T

Y

G

T

I

V

V

L

P

D

F

M

D

R

R

D

L

I

S

L

V

A

P

S

D

D

P

A

I

V

A

A

L

V

G

T

V

F

-

A

-

D

D

Q

V

I

I

F

G

G

L

I

-

F

R

N

W

W

L

I

S

I

T

P

V

L

-

S

V

V

K

A

L

L

G

S

A

C

I

F

L

G

G

G

L

L

S

N

S

A

V

S

V

I

L

-

V

-

L

-

L

V

G

A

Q

A

T

S

R

R

V

L

L

F

Y

F

S

V

I

G

A

S

R

R

D

E

G

G

L

H

L

L

P

P

P

D

I

A

A

I

A

C

K

M

V

I

H

H

P

V

R

E

F

R

R

F

T

T

P

P

Y

V

L

P

T

S

T

L

I

L

G

F

T

N

G

G

L

I

I

M

V

A

A

L

V

I

M

Y

A

L

G

C

V

V

L

E

P

D

I

I

G

F

L

Q

V

L

G

I

E

N

L

Y

V

Y

S

S

I

F

G

S

T

Y

L

W

F

F

A

F

F

V

A

G

I

L

V

S

C

I

A

V

G

G

V

Q

L

L

F

Y

L

L

R

R

Y

W

T

K

H

E

P

P

Q

D

I

R

H

P

R

R

P

P

F

L

R

K

A

-

P

-

L

-

I

L

A

S

I

V

V

F

A

F

P

P

L

I

G

V

A

F

A

C

A

L

A

C

V

T

V

I

L

F

M

L

L

V

G

A

L

V

P

P

-

L

-

Y

R

S

D

D

T

T

W

-

I

I

R

N

F

S

A

L

I

I

W

G

L

I

A

A

I

I

-

A

-

L

-

S

G

G

L

L

I

P

L

F

Y

Y

F

F

T

L

Y

I

G

I

R

R

-

V

-

P

R

E

H

H

S

K

R

R

binding leucine: N21 (≠ C45), M22 (≠ I46), I23 (= I47), G24 (= G48), S25 (≠ A49), G26 (= G50), S210 (≠ A261), S212 (≠ I263), G213 (= G264)

8xyjA Structure of y+lat1 bound with lys
24% identity, 94% coverage: 25:488/494 of query aligns to 1:440/459 of 8xyjA

query
sites
8xyjA

E

E

T

Q

P

V

N

K

L

L

R

K

T

E

L

I

S

S

L

L

A

L

S

N

L

G

I

V

S

C

L

L

G

I

I

V

G

G

C
x
N

I
x
M

I

I

G
|
G

A

S

G

G

I

I

F

F

V

V

L

S

T

P

G

K

H

G

A

V

A

L

A

I

A

Y

Y

S

A

A

G

S

P

F

A

G

I

L

S

S

L

L

S

V

-

I

F

W

V

A

L

V

A

G

G

G

L

L

V

F

C

S

A

V

L

F

A

G

G

A

L

L

C

C

Y

Y

A

A

E

E

M

L

A

G

S

T

T

T

V

I

P

K

V

K

A

S

G

G

S

A

A

S

Y

Y

T

A

Y

Y

A

I

Y

L

A

E

T

A

L

F

G

G

E

G

F

F

I

L

A

A

W

F

I

I

I

R

G

L

W

W

-

T

D

S

L

L

L

I

E

I

Y

E

A

P

F

T

G

S

A

Q

T

A

T

I

V

I

A

A

I

I

G

T

W

F

S

A

G

N

Y

Y

V

M

V

V

S

Q

F

P

L

L

R

-

D

-

F

-

H

-

I

-

G

-

I

-

P

-

A

-

L

-

A

-

G

-

A

F

P

P

F

S

A

C

F

F

D

A

P

P

A

Y

S

A

G

A

A

S

W

-

T

-

H

-

T

-

G

-

A

-

L

-

V

-

N

R

A

L

P

L

A

A

V

A

A

A

I

C

V

I

L

C

A

L

L

L

T

T

A

F

L

I

L

N

V

C

V

A

G

Y

V

V

N

K

E

W

S

G

A

T

K

L

V

V

N

Q

N

D

I

I

I

F

V

T

A

Y

I

A

K

K

L

V

A

L

I

A

I

L

V

I

V

A

F

V

I

I

L

V

A

A

G

G

L

I

S

V

S

R

V

L

S

G

T

Q

A

G

N

-

W

-

V

-

T

A

S

S

A

T

N

H

P

F

D

E

G

N

A

S

F

F

I

E

P

G

P

S

N

S

A

F

G

A

P

V

G

G

E

D

Y

-

G

-

W

-

S

-

G

-

I

I

L

A

R

L

G

A

A

L

A

Y

V

S

V

A

F

L

F

F

A

S

Y

Y

I
x
S

G
|
G

F

W

D

D

A

T

V

L

S

N

T

Y

A

V

A

T

Q

E

E

E

A

I

K

K

N

N

P

P

Q

E

R

R

D

N

M

L

P

P

L

L

G

S

I

I

L

G

G

I

S

S

L

M

V

P

I

I

C

V

T

T

V

I

L

I

Y

Y

V

I

L

L

V

T

S

N

I

V

V

A

I

Y

T

Y

G

T

I

V

V

L

P

D

F

M

D

R

R

D

L

I

S

L

V

A

P

S

D

D

P

A

I

V

A

A

L

V

G

T

V

F

-

A

-

D

D

Q

V

I

I

F

G

G

L

I

-

F

R

N

W

W

L

I

S

I

T

P

V

L

-

S

V

V

K

A

L

L

G

S

A

C

I

F

L

G

G

G

L

L

S

N

S

A

V

S

V

I

L

-

V

-

L

-

L

V

G

A

Q

A

T

S

R

R

V

L

L

F

Y

F

S

V

I

G

A

S

R

R

D

E

G

G

L

H

L

L

P

P

P

D

I

A

A

I

A

C

K

M

V

I

H

H

P

V

R

E

F

R

R

F

T

T

P

P

Y

V

L

P

T

S

T

L

I

L

G

F

T

N

G

G

L

I

I

M

V

A

A

L

V

I

M

Y

A

L

G

C

V

V

L

E

P

D

I

I

G

F

L

Q

V

L

G

I

E

N

L

Y

V

Y

S

S

I

F

G

S

T

Y

L

W

F

F

A

F

F

V

A

G

I

L

V

S

C

I

A

V

G

G

V

Q

L

L

F

Y

L

L

R

R

Y

W

T

K

H

E

P

P

Q

D

I

R

H

P

R

R

P

P

F

L

R

K

A

-

P

-

L

-

I

L

A

S

I

V

V

F

A

F

P

P

L

I

G

V

A

F

A

C

A

L

A

C

V

T

V

I

L

F

M

L

L

V

G

A

L

V

P

P

-

L

-

Y

R

S

D

D

T

T

W

-

I

I

R

N

F

S

A

L

I

I

W

G

L

I

A

A

I

I

-

A

-

L

-

S

G

G

L

L

I

P

L

F

Y

Y

F

F

T

L

Y

I

G

I

R

R

-

V

-

P

R

E

H

H

S

K

R

R

binding lysine: N21 (≠ C45), M22 (≠ I46), G24 (= G48), S212 (≠ I263), G213 (= G264)

Q9QXW9 Large neutral amino acids transporter small subunit 2; L-type amino acid transporter 2; mLAT2; Solute carrier family 7 member 8 from Mus musculus (Mouse) (see paper)
26% identity, 86% coverage: 19:442/494 of query aligns to 25:424/531 of Q9QXW9

query
sites
Q9QXW9

E

E

A

A

E

S

F

S

G

G

S

G

E

G

T

G

P

V

N

A

L

L

R

K

T

E

L

I

S

G

L

L

A

V

S

S

L

A

I

C

S

G

L

I

G

I

I

V

G

G

C

N

I

I

G

G

A

S

G

G

I

I

F

F

V

V

L

S

T

P

G

K

H

G

A

V

A

L

A

E

A

N

Y

A

A

G

G

S

P

V

A

G

I

L

S

A

L

L

-

I

S

V

F

W

V

I

L

V

A

T

G

G

L

I

V

I

C

T

A

A

L

V

A

G

G

A

L

L

C

C

Y

Y

A

A

E

E

M

L

A

G

S

V

T

T

V

I

P

P

V

K

A

S

G

G

S

G

A

D

Y

Y

T

S

Y

Y

A

V

Y

K

A

D

T

I

L

F

G

G

E

G

F

L

I

A

A

G

W

F

I

L

I

R

G

L

W

W

-

I

D

A

L

V

L

L

L

V

E

I

Y
|
Y

A

P

F

T

G
x
N

A

Q

T

A

T

V

V

I

A

A

I

L

G

T

W

F

S

S

G

N

Y

Y

V

V

V

L

S

Q

F

P

L

L

R

-

D

-

F

-

H

-

I

-

G

-

I

-

P

-

A

-

L

-

A

-

G

-

A

F

P

P

F

T

A

C

F

F

D

P

P

P

A

E

S

S

G

G

A

L

W

-

T

-

H

-

T

-

G

-

A

-

L

-

V

-

N

R

A

L

P

L

A

A

V

A

A

I

I

C

V

L

L

L

A

L

L

L

T

T

A

W

L

V

L

N

V

C

V

S

G

S

V

V

N

R

E

W

S

A

A

T

K

R

V

V

N

Q

N

D

I

I

I

F

V

T

A

A

I

G

K

K

L

L

A

L

I

A

I

L

V

A

V

L

F

I

I

I

L

I

A

M

G

G

L

I

S

V

S

Q

V

I

S

C

T

K

A

G

N

E

-

F

W

W

V

L

T

E

S

P

A

K

N

N

P

A

D

F

G

E

A

N

F

F

I

Q

P

E

P

P

N

D

A

I

G

G

P

L

G

V

E

A

Y

L

G

A

W

F

S

-

G

-

I

-

L

L

R

Q

G

G

A

S

A

-

V

-

F

-

F
|
F

A

A

Y

Y

I

G

G

G

F

W

D

N

A

F

V

L

S

N

T

Y

A

V

A

T

Q

E

E

E

A

L

K

V

N

D

P

P

Q

Y

R

K

D

N

M

L

P

P

L

R

G

A

I

I

L

F

G

I

S

S

L

I

V

P

I

L

C

V

T

T

V

F

L

V

Y

Y

V

V

L

F

V

A

S

N

I

I

V

A

-

Y

I

V

T

T

G

A

I

M

V

S

P

P

F

Q

D

E

R

L

L

L

-

A

S

S

V

N

P

A

D

V

P

A

I

V

A

T

L

F

G

G

V

E

D

K

V

L

I

L

G

G

-

V

L

M

R

A

W

W

L

I

S

M

T

P

V

I

-

S

V

V

K

A

L

L

G

S

A

T

I

F

L

G

G

G

L

V

S

N

S

G

V

S

V

-

L

-

V

-

L

-

L

L

L

F

G

T

Q

S

T

S

R

R

V

L

L

F

Y

F

S

A

I

G

A

A

R

R

D

E

G

G

L

H

L

L

P

P

P

S

I

V

A

L

A

A

K

M

V

I

H

H

P

V

R

K

F

R

R

C

T

T

P

P

-

I

-

P

-

A

-

L

Y

L

L

F

T

T

T

C

I

L

G

S

T

T

G

L

L

L

I

M

V

L

A

V

V

T

M

S

A

D

G

M

V

Y

L

T

P

L

I

I

G

N

L

Y

V

V

G

G

E

-

L

-

V

-

S

F

I

I

G

N

T

Y

L

L

F

F

A

-

F

Y

A

G

I

V

V

T

C

V

A

A

G

G

V

Q

L

I

F

V

L

L

R

R

Y

W

T

K

H

K

P

P

Q

D

I

I

H

P

R

R

P

P

F

I

R

K

A

V

P

S

L

L

I

L

Y130 (= Y122) mutation to A: Increases T2 import. Increases T3 and enables T4 import. Does not affect L-leucine and L-phenylalanine uptake.
N133 (≠ G125) mutation to S: Increases T2 import. Does not affect T3 import. Does not affect L-leucine and L-phenylalanine uptake. Increases the export of both L-leucine and L-phenylalanine.
F242 (= F260) mutation to W: Increases T2 import. Does not affect T3 import. Does not affect L-leucine and L-phenylalanine uptake.

P82251 b(0,+)-type amino acid transporter 1; b(0,+)AT1; Glycoprotein-associated amino acid transporter b0,+AT1; Solute carrier family 7 member 9 from Homo sapiens (Human) (see 11 papers)
23% identity, 95% coverage: 14:482/494 of query aligns to 8:454/487 of P82251

query
sites
P82251

K

K

A

R

E

R

A

E

H

D

E

E

A

K

E

S

F

I

G

Q

S

S

E

Q

T

E

P

P

N

K

-

T

-

S

L

L

R

Q

R

K

T

E

L

L

S

G

L

L

A

I

S

S

L

G

I

I

S

S

L

I

G

I

I
x
V

G

G

C

T

I
|
I

G
|
G

A
x
S

G
|
G

I

I

F

F

V

V

L
x
S

T
x
P

G

K

H

S

-

V

-

L

A

S

A

N

A

T

A

E

Y

A

A

V

G

G

P

P

A

C

I

L

S

I

L

I

S

-

F

W

V
x
A

L

A

A

C

G

G

L

V

V

L

C

A

A

T

L

L

A

G

G

A

L

L

C

C

Y

F

A

A

E

E

M

L

A

G

S

T

T

M

V

I

P

T

V

K

A

S

G

G

S

G

A

E

Y

Y

T

P

Y
|
Y

A

L

Y

M

A

E

T

A

L

Y

G
|
G

E

P

F

I

I

P

A

A

W

Y

I

L

I

F

G

S

W
|
W

-

A

D

S

L

L

L

I

L

V

E
x
I

Y

K

A

P

F
x
T

G

S

A

F

T

A

T

I

V

I

A

C

I

L

G

S

W

F

S

S

G

E

Y

Y

V

V

V

C

S

A

F

-

L

-

R

-

D

P

F

F

H

Y

I
x
V

G

G

I
x
C

P

-

A

-

L

-

A

-

G

-

A

-

P

-

F

-

A

-

F

-

D

K

P

P

A

P

S

Q

G

-

A

-

W

-

T

-

H

-

T

-

G

-

A

I

L

V

V

V

N

K

A

C

P

L

A

A

V

A

A

A

I

A

V

I

L

L

A

F

L

I

T

S

A

T

L

V

L

N

V

S

V

L

G

S

V
|
V

N

R

E

L

S

G

A

S

K

Y

V

V

N

Q

N

N

I

I

I

F

V

T

A
|
A

I

A

K

K

L

L

A

V

I

I

I

V

V

A

V

I

F

I

I

I

L

I

A

S

G
|
G

L

L

S

V

S

L

V

L

S

A

T

Q

A

G

N

N

W

-

V

-

T

-

S

T

A

K

N

N

P

F

D

D

G

N

A

S

F

F

I

E

P

G

P

A

N

Q

A

L

G

S

P

V

G

G

E

A

Y

I

G

S

-
x
L

-
x
A

-

F

W

Y

S
x
N

G

G

I

L

L

-

R

-

G

-

A

-

V

-

F

-

F
x
W

A

A

Y

Y

I
x
D

G

G

F
x
W

D

N

A
x
Q

V

L

S

N

T

Y

A

I

A

T

Q

E

E

E

A

L

K

R

N

N

P

P

Q

Y

R

R

D

N

M

L

P

P

L

L

G

A

I

I

L

I

G

I

S
x
G

L

I

V
x
P

I

L

C

V

T

T

V

A

L

C

Y

Y

V

I

L

L

V

M

S

N

I

V

V

S

I

Y

T

F

G

T

I

V

V

M

P

T

F

A

D

T

R

E

L

L

S

L

V

Q

P
x
S

D

Q

P

A

I

V

A

A

L

V

G

T

V

F

D

G

V

D

I

R

G

V

L

L

R

Y

W

P

L

A

S

S

T

W

V

I

V

V

K

P

L

L

G

F

A

V

I

A

L

F

G

S

L

T

S

I

S

G

V

A

L

N

V

G

L

T

L
x
C

L

F

G

T

Q
x
A

T

G

R

R

V

L

L

I

Y

Y

S
x
V

I
x
A

A

G

R
|
R

D

E

G

G

L

H

L

M

P

L

P

K

I

V

A

L

A

S

K

Y

V

I

H

S

P

V

R

R

F

R

R

L

T

T

P

P

Y

A

L

P

T
x
A

T

I

I

I

G

F

T

Y

G

G

L

I

I

I

V

A

A

T

V

I

M

Y

A

-

G

-

V

I

L

I

P

P

I

-

G

-

L

-

V

-

G

G

E

D

L

I

V

N

S

S

I

L

G

V

T

N

L

Y

F

F

A
x
S

F

F

A

A

-
x
A

-
x
W

-

L

-

F

-
x
Y

-

G

I

L

V

T

C

I

A

L

G

G

V

L

L

I

F

V

L

M

R

R

Y

F

T

T

H

R

P
x
K

Q

E

I

L

H

E

R

R

P

P

F

I

R

K

A

V

P

P

L

V

I

V

A

I

I

P

V

V

A

L

P

M

L

T

G

L

A

I

A

S

A

V

A

F

V

L

V

V

L
|
L

M

A

L

P

G

I

L

I

P

S

R

K

D

P

T

T

W

W

-

E

-

Y

I

L

R

Y

F

C

A

V

I

L

W

F

L

I

A

L

I

S

G

G

L

L

I

L

L

F

Y

Y

F

F

T

L

Y

F

V40 (≠ I43) to M: in CSNU; uncertain significance
IIGSG 43:47 (≠ IIGAG 46:50) binding L-arginine
I44 (= I47) to T: in CSNU; type I; dbSNP:rs121908485
S51 (≠ L54) to F: in CSNU; uncertain significance
P52 (≠ T55) to L: in CSNU; impairs protein stability and dimer formation; dbSNP:rs1198613438
A70 (≠ V72) to V: in CSNU; partial loss of amino acid transport activity; dbSNP:rs769448665
Y99 (= Y101) to H: in CSNU; uncertain significance
G105 (= G107) to R: in CSNU; type III; severe loss of amino acid transport activity; dbSNP:rs121908480
W114 (= W116) to R: in CSNU; uncertain significance
I120 (≠ E121) to L: in CSNU; uncertain significance
T123 (≠ F124) to M: in CSNU; partial loss of amino acid transport activity; dbSNP:rs79987078
V142 (≠ I146) to A: no effect on amino acid transport activity; dbSNP:rs12150889
C144 (≠ I148) modified: Interchain (with C-114 in SLC3A1)
V170 (= V192) to M: in CSNU; type III; severe loss of amino acid transport activity; dbSNP:rs121908479
A182 (= A204) to T: in CSNU; type III; partial loss of amino acid transport activity; dbSNP:rs79389353
G195 (= G217) to R: in CSNU; type III; decreased amino acid transport activity; dbSNP:rs121908482
L223 (vs. gap) to M: slightly decreased amino acid transport activity; dbSNP:rs1007160
A224 (vs. gap) to V: in CSNU; non-classic type I; dbSNP:rs140873167
N227 (≠ S249) to D: in CSNU; decreased amino acid transport activity
W230 (≠ F260) to R: in CSNU; complete loss of amino acid transport activity; mutation to A: Abolishes amino acid transport activity.
D233 (≠ I263) binding L-arginine; mutation to A: Complete loss of amino acid transport activity.
W235 (≠ F265) mutation to A: Complete loss of amino acid transport activity.
Q237 (≠ A267) mutation to A: Reduces amino acid transport activity.
G259 (≠ S289) to R: in CSNU; type III; impairs protein stability and dimer formation; dbSNP:rs121908483
P261 (≠ V291) to L: in CSNU; types I and III; dbSNP:rs121908486
S286 (≠ P316) to F: in CSNU; uncertain significance; dbSNP:rs755135545
C321 (≠ L351) mutation to S: Does not affect amino acid transport activity.
A324 (≠ Q354) to E: in CSNU; uncertain significance
V330 (≠ S360) to M: in CSNU; type III; dbSNP:rs201618022
A331 (≠ I361) to V: in CSNU; non-classic type I; dbSNP:rs768466784
R333 (= R363) to Q: in CSNU; decreased amino acid transport activity; dbSNP:rs769576205; to W: in CSNU; severe loss of amino acid transport activity; dbSNP:rs121908484
A354 (≠ T384) to T: in CSNU; type III; severe loss of amino acid transport activity; dbSNP:rs939028046
S379 (≠ A415) mutation to A: Markedly reduces amino acid transport activity.
A382 (vs. gap) to T: in CSNU; severe loss of amino acid transport activity; dbSNP:rs774878350
W383 (vs. gap) mutation to A: Complete loss of amino acid transport activity.
Y386 (vs. gap) mutation to A: Loss of amino acid transport activity.
K401 (≠ P431) to E: in CSNU; uncertain significance; dbSNP:rs760264924
L426 (= L456) to P: in CSNU; uncertain significance

Sites not aligning to the query:

482 P → L: in CSNU; severe loss of amino acid transport activity; no effect on localization to the apical membrane; dbSNP:rs146815072; mutation P->A,G,S,V: No effect on amino acid transport activity.; mutation P->F,I,M,W: Decreased amino acid transport activity.

Q9UHI5 Large neutral amino acids transporter small subunit 2; L-type amino acid transporter 2; hLAT2; Solute carrier family 7 member 8 from Homo sapiens (Human) (see 3 papers)
25% identity, 84% coverage: 29:442/494 of query aligns to 36:425/535 of Q9UHI5

query
sites
Q9UHI5

L

L

R

K

T

E

L

I

S

G

L

L

A

V

S

S

L

A

I

C

S

G

L

I

G

I

I

V

G

G

C

N

I
|
I

I

I

G

G

A

S

G

G

I

I

F

F

V

V

L

S

T

P

G

K

H

G

A

V

A

L

A

E

A

N

Y

A

A

G

G

S

P

V

A

G

I

L

S

A

L

L

-

I

S

V

F

W

V

I

L

V

A

T

G

G

L

F

V

I

C

T

A

V

L

V

A

G

G

A

L

L

C

C

Y
|
Y

A

A

E

E

M

L

A

G

S

V

T

T

V

I

P

P

V

K

A

S

G

G

S

G

A

D

Y

Y

T

S

Y

Y

A

V

Y

K

A

D

T

I

L

F

G

G

E

G

F

L

I

A

A

G

W

F

I

L

I

R

G

L

W

W

-

I

D

A

L

V

L

L

L

V

E

I

Y

Y

A

P

F

T

G
x
N

A

Q

T

A

T

V

V

I

A

A

I

L

G

T

W

F

S

S

G

N

Y

Y

V

V

V

L

S

Q

F

P

L

L

R

-

D

-

F

-

H

-

I

-

G

-

I

-

P

-

A

-

L

-

A

-

G

-

A

F

P

P

F

T

A
x
C

F

F

D

P

P

P

A

E

S

S

G

G

A

L

W

-

T

-

H

-

T

-

G

-

A

-

L

-

V

-

N

R

A

L

P

L

A

A

V

A

A

I

I

C

V

L

L

L

A

L

L

L

T

T

A
x
W

L

V

L

N

V

C

V

S

G

S

V

V

N

R

E

W

S

A

A

T

K

R

V

V

N

Q

N

D

I

I

I

F

V

T

A

A

I

G

K

K

L

L

A

L

I

A

I

L

V

A

V

L

F

I

I

I

L

I

A

M

G

G

L

I

S

V

S

Q

V

I

S

C

T

K

A

G

N

E

-

Y

-

F

W

W

V

L

T

E

S

P

A

K

N

N

P

A

D

F

G

E

A

N

F

F

I

Q

P

E

P

P

N

D

A

I

G

G

P

L

G

V

E

A

Y

L

G

A

W

F

S

-

G

-

I

-

L

L

R

Q

G

G

A

S

A

-

V

-

F

-

F
|
F

A

A

Y

Y

I
x
G

G

G

F

W

D

N

A

F

V

L

S

N

T

Y

A

V

A

T

Q

E

E

E

A

L

K

V

N

D

P

P

Q

Y

R

K

D

N

M

L

P

P

L

R

G

A

I

I

L

F

G

I

S

S

L

I

V

P

I

L

C

V

T

T

V

F

L

V

Y

Y

V

V

L

F

V

A

S

N

I

V

V

A

-

Y

I

V

T

T

G

A

I

M

V

S

P

P

F

Q

D

E

R

L

L

L

-

A

S

S

V

N

P

A

D
x
V

P

A

I

V

A

T

L

F

G

G

V

E

D

K

V

L

I

L

G

G

-

V

L

M

R

A

W

W

L

I

S

M

T

P

V

I

-

S

V

V

K

A

L

L

G

S

A

T

I

F

L

G

G

G

L

V

S

N

S

G

V

S

V

-

L

-

V

-

L

-

L

L

L

F

G

T

Q

S

T

S

R

R

V

L

L

F

Y

F

S

A

I

G

A

A

R

R

D

E

G

G

L

H

L

L

P

P

P

S

I

V

A

L

A

A

K

M

V

I

H

H

P

V

R

K

F

R

R

C

T

T

P

P

-

I

-

P

-

A

-

L

Y

L

L

F

T

T

T

C

I

I

G

S

T

T

G

L

L

L

I

M

V

L

A

V

V

T

M

S

A

D

G

M

V

Y

L

T

P

L

I

I

G

N

L

Y

V

V

G

G

E

-

L

-

V

-

S

F

I

I

G
x
N

T
x
Y

L

L

F

F

A

-

F

Y

A

G

I

V

V
x
T

C

V

A

A

G

G

V

Q

L

I

F

V

L

L

R

R

Y

W

T

K

H

K

P

P

Q

D

I

I

H

P

R
|
R

P

P

F

I

R

K

A

I

P

N

L

L

I

L

I53 (= I46) binding L-leucine
Y93 (= Y85) mutation to A: Nearly complete reduction of glycine, L-alanine, and L-glutamine uptake. Minimal effect on the transport of L-isoleucine, L-histidine and L-tryptophan.
N134 (≠ G125) Important for substrate specificity; binding L-tryptophan; mutation to Q: Reduces L-leucine uptake activity. Abolishes L-tryptophan uptake.; mutation to S: The substrate specificity changed dramatically reducing L-glutamine, glycine and L-alanine uptake activity thus mimicking the selectivity of SLC7A5.
C154 (≠ A158) modified: Interchain (with C-210 in SLC3A2)
W174 (≠ A186) mutation to A: Does not affect protein expression, plasma membrane localization, or L-alanine uptake.
F243 (= F260) mutation to A: Abolishes leucine and tryptophan transport activities.
G246 (≠ I263) Important for substrate specificity; binding L-leucine; mutation to S: Strong decrease in the uptake of large substrates L-tryptophan, L-glutamine, and L-histidine but increases the uptake of small neutral amino acids glycine and L-alanine.
V302 (≠ D317) to I: found in a patient with age-related hearing loss; does not affect L-alanine transport activity. Decreases L-tyrosine transport activity; dbSNP:rs142951280
N395 (≠ G411) binding L-tryptophan; mutation to Q: Strongly reduces L-leucine uptake activity. Strongly reduces L-tryptophan uptake activity.
Y396 (≠ T412) mutation to A: Strongly reduces L-leucine uptake activity.
T402 (≠ V419) to M: found in a patient with age-related hearing loss; strongly decreased L-alanine transport activity. Decreases L-tyrosine transport activity; dbSNP:rs758342760
R418 (= R435) to C: found in a patient with age-related hearing loss; decreases L-alanine transport activity. Decreases L-tyrosine transport activity; dbSNP:rs146946494

Sites not aligning to the query:

460 V → E: found in a patient with age-related hearing loss; strongly decreases L-alanine transport activity. Decreases L-tyrosine transport activity. Decreases cell membrane localization; dbSNP:rs2048595742

Q01650 Large neutral amino acids transporter small subunit 1; 4F2 light chain; 4F2 LC; 4F2LC; CD98 light chain; Integral membrane protein E16; E16; L-type amino acid transporter 1; hLAT1; Solute carrier family 7 member 5; y+ system cationic amino acid transporter from Homo sapiens (Human) (see 3 papers)
25% identity, 92% coverage: 25:480/494 of query aligns to 42:473/507 of Q01650

query
sites
Q01650

E

E

T

G

P

V

N

T

L

L

R

Q

R

R

T

N

L

I

S

T

L

L

A

L

S

N

L

G

I

V

S

A

L

I

G

I

I

V

G

G

C

T

I

I

G

G

A

S

G

G

I

I

F

F

V

V

L

T

T

P

G

T

H

G

A

V

A

L

A

K

A

E

Y

A

A

G

G

S

P

P

A

G

I

L

S

A

L

L

S

V

-

V

F

W

V

A

L

A

A

C

G

G

L

V

V

F

C

S

A

I

L

V

A

G

G

A

L

L

C

C

Y

Y

A

A

E

E

M

L

A

G

S

T

T

T

V

I

P

S

V

K

A

S

G

G

S

G

A

D

Y
|
Y

T

A

Y

Y

A

M

Y

L

A

E

T

V

L

Y

G

G

E

S

F

L

I

P

A

A

W

F

I

L

I

K

G

L

W

W

-

I

D

E

L

L

L

I

E

I

Y

R

A

P

F

S

G

S

A

Q

T

Y

T

I

V

V

A

A

I

L

G

V

W

F

S

A

G

T

Y

Y

V

L

V

-

S

-

F

-

L

L

R

K

D

P

F

L

H

F

I

P

G

T

I
x
C

P

P

A

V

A

-

L

-

A

-

G

-

A

-

P

-

F

-

A

-

F

-

D

-

P

P

A

E

S

E

G

A

A

A

W

-

T

-

H

-

T

-

G

-

A

-

L

-

V

-

N

K

A

L

P

V

A

A

V

C

A

L

I

C

V

V

L

L

A

L

L

L

T

T

A

A

L

V

L

N

V

C

V

Y

G

S

V

V

N

K

E

A

S

A

A

T

K

R

V

V

N

Q

N

D

I

A

I

F

V

A

A

A

I

A

K

K

L

L

A

L

I

A

I

L

V

A

V

L

F

I

I

I

L

L

A

L

G

G

L

F

S

V

S

Q

V

I

S

G

T

K

A

G

N
x
D

W

-

V

V

T

S

S

N

A

L

N

D

P

P

D
x
N

G

F

A

S

F

F

I

E

P

G

P

T

N

K

A

L

G

D

P

V

G

G

E

N

Y

-

G

-

W

-

S

-

G

-

I

I

L

V

R

L

G
x
A

A

L

A

Y

V

S

V

G

F

L

F
|
F

A

A

Y

Y

I

G

G

G

F
x
W

D
x
N

A
x
Y

V

L

S

N

T

F

A

V

A

T

Q

E

E

E

A

M

K

I

N

N

P

P

Q

Y

R

R

D

N

M

L

P

P

L

L

G

A

I

I

L

I

G

I

S

S

L

L

V

P

I

I

C

V

T

T

V

L

L

V

Y

Y

V

V

L

L

V

T

S

N

I

L

V

A

I

Y

T

F

G

T

I

T

V

L

P

S

F

T

D
x
E

R

Q

L

M

S

L

V

S

P

S

D

E

P

A

I

V

A

A

L

V

G

D

V

F

D

G

V

N

I

Y

G

H

L

L

R

G

W

V

L

M

S

S

T

W

V

I

K

P

L

V

G

F

A

V

I

G

L

L

G

S

L

C

S

F

S

G

V

S

V

V

L

N

V

G

L

S

L

L

L

F

G

T

Q

S

T

S

R

R

V

L

L

F

Y

F

S

V

I

G

A

S

R

R

D

E

G

G

L

H

L

L

P

P

P

S

I

I

A

L

A

S

K

M

V

I

H

H

P

P

R

Q

F

L

R

L

T

T

P

P

Y

V

L
x
P

T

S

T

L

I

V

G

F

T

T

G

C

L

V

I

M

V

T

A

L

V

L

M

Y

A

A

G

F

V

S

L

K

P

D

I

I

G

F

L

S

V

V

G

I

E

N

L

F

V

F

S

S

I

F

G

F

T

N

L

W

F

L

A

C

F

V

A

A

I

L

V

A

C

I

A

I

G

G

V

M

L

I

F

W

L

L

R

R

Y

H

T

R

H

K

P

P

Q

E

I

L

H

E

R

R

P

P

F

I

R

K

A

V

-

N

-

L

-

A

-

L

P

P

L

V

I

F

A

F

I

I

V

L

A

A

P

C

L

L

G

F

A

L

A

I

A

A

A

-

V

-

V

V

L

S

M

F

L

W

G

K

L

T

P

P

R

V

D

E

T

C

W

G

I

I

R

G

F

F

A

T

I

I

W

I

L

L

A

S

I

-

G

G

L

L

I

P

L

V

Y

Y

F

F

Y117 (= Y99) mutation to A: Strongly decreased leucine transport activity.
C164 (≠ I148) modified: Interchain (with C-210 in SLC3A2)
D223 (≠ N225) to V: in dbSNP:rs17853937
N230 (≠ D233) to K: in dbSNP:rs1060250
A246 (≠ G254) mutation to V: Nearly abolishes leucine transport activity.
F252 (= F260) mutation to A: Nearly abolishes leucine transport activity.
W257 (≠ F265) mutation to A: Nearly abolishes leucine transport activity.
N258 (≠ D266) mutation to A: Decreased leucine transport activity.; mutation to D: Nearly abolishes leucine transport activity.
Y259 (≠ A267) mutation to A: Strongly decreased leucine transport activity.
E303 (≠ D311) mutation to K: Decreased leucine transport activity.
P375 (≠ L383) mutation to L: Nearly abolishes leucine transport activity.

Sites not aligning to the query:

483:507 mutation Missing: Nearly abolishes leucine transport activity.

8j8mB Overall structure of the lat1-4f2hc bound with tryptophan
25% identity, 91% coverage: 29:480/494 of query aligns to 3:430/463 of 8j8mB

query
sites
8j8mB

L

L

R

Q

R

R

T

N

L

I

S

T

L

L

A

L

S

N

L

G

I

V

S

A

L

I

G

I

I

V

G

G

C

T

I
|
I

I

I

G
|
G

A
x
S

G

G

I

I

F

F

V

V

L

T

T

P

G

T

H

G

A

V

A

L

A

K

A

E

Y

A

A

G

G

S

P

P

A

G

I

L

S

A

L

L

S

V

-

V

F

W

V

A

L

A

A

C

G

G

L

V

V

F

C

S

A

I

L

V

A

G

G

A

L

L

C

C

Y

Y

A

A

E

E

M

L

A

G

S

T

T

T

V

I

P

S

V

K

A

S

G

G

S

G

A

D

Y

Y

T

A

Y

Y

A

M

Y

L

A

E

T

V

L

Y

G

G

E

S

F

L

I

P

A

A

W

F

I

L

I

K

G

L

W

W

-

I

D

E

L

L

L

I

E

I

Y

R

A

P

F

S

G

S

A

Q

T

Y

T

I

V

V

A

A

I

L

G

V

W

F

S

A

G

T

Y

Y

V

L

V

-

S

-

F

-

L

L

R

K

D

P

F

L

H

F

I

P

G

T

I

C

P

P

A

V

A

-

L

-

A

-

G

-

A

-

P

-

F

-

A

-

F

-

D

-

P

P

A

E

S

E

G

A

A

A

W

-

T

-

H

-

T

-

G

-

A

-

L

-

V

-

N

K

A

L

P

V

A

A

V

C

A

L

I

C

V

V

L

L

A

L

L

L

T

T

A

A

L

V

L

N

V

C

V

Y

G

S

V

V

N

K

E

A

S

A

A

T

K

R

V

V

N

Q

N

D

I

A

I

F

V

A

A

A

I

A

K

K

L

L

A

L

I

A

I

L

V

A

V

L

F

I

I

I

L

L

A

L

G

G

L

F

S

V

S

Q

V

I

S

G

T

K

A

G

N

D

W

-

V

V

T

S

S

N

A

L

N

D

P

P

D

N

G

F

A

S

F

F

I

E

P

G

P

T

N

K

A

L

G

D

P

V

G

G

E

N

Y

-

G

-

W

-

S

-

G

-

I

I

L

V

R

L

G

A

A

L

A

Y

V

S

V

G

F

L

F
|
F

A

A

Y

Y

I

G

G

G

F

W

D

N

A

Y

V

L

S

N

T

F

A

V

A

T

Q

E

E

E

A

M

K

I

N

N

P

P

Q

Y

R

R

D

N

M

L

P

P

L

L

G

A

I

I

L

I

G

I

S

S

L

L

V

P

I

I

C

V

T

T

V

L

L

V

Y

Y

V

V

L

L

V

T

S

N

I

L

V

A

I

Y

T

F

G

T

I

T

V

L

P

S

F

T

D

E

R

Q

L

M

S

L

V

S

P

S

D

E

P

A

I

V

A

A

L

V

G

D

V

F

D

G

V

N

I

Y

G

H

L

L

R

G

W

V

L

M

S

S

T

W

V

I

K

P

L

V

G

F

A

V

I

G

L

L

G

S

L

C

S

F

S

G

V

S

V

V

L

N

V

G

L

S

L

L

L

F

G

T

Q

S

T

S

R

R

V

L

L

F

Y

F

S

V

I

G

A

S

R

R

D

E

G

G

L

H

L

L

P

P

P

S

I

I

A

L

A

S

K

M

V

I

H

H

P

P

R

Q

F

L

R

L

T

T

P

P

Y

V

L

P

T

S

T

L

I

V

G

F

T

T

G

C

L

V

I

M

V

T

A

L

V

L

M

Y

A

A

G

F

V

S

L

K

P

D

I

I

G

F

L

S

V

V

G

I

E

N

L

F

V
x
F

S

S

I

F

G

F

T

N

L

W

F

L

A

C

F

V

A

A

I

L

V

A

C

I

A

I

G

G

V

M

L

I

F

W

L

L

R

R

Y

H

T

R

H

K

P

P

Q

E

I

L

H

E

R

R

P

P

F

I

R

K

A

V

-

N

-

L

-

A

-

L

P

P

L

V

I

F

A

F

I

I

V

L

A

A

P

C

L

L

G

F

A

L

A

I

A

A

A

-

V

-

V

V

L

S

M

F

L

W

G

K

L

T

P

P

R

V

D

E

T

C

W

G

I

I

R

G

F

F

A

T

I

I

W

I

L

L

A

S

I

-

G

G

L

L

I

P

L

V

Y

Y

F

F

binding tryptophan: I20 (= I46), G22 (= G48), S23 (≠ A49), F209 (= F260), F357 (≠ V408)

8j8lB Overall structure of the lat1-4f2hc bound with l-dopa
25% identity, 91% coverage: 29:480/494 of query aligns to 3:430/463 of 8j8lB

query
sites
8j8lB

L

L

R

Q

R

R

T

N

L

I

S

T

L

L

A

L

S

N

L

G

I

V

S

A

L

I

G

I

I

V

G

G

C

T

I
|
I

I

I

G
|
G

A
x
S

G
|
G

I

I

F

F

V

V

L

T

T

P

G

T

H

G

A

V

A

L

A

K

A

E

Y

A

A

G

G

S

P

P

A

G

I

L

S

A

L

L

S

V

-

V

F

W

V

A

L

A

A

C

G

G

L

V

V

F

C

S

A

I

L

V

A

G

G

A

L

L

C

C

Y

Y

A

A

E

E

M

L

A

G

S

T

T

T

V

I

P

S

V

K

A

S

G

G

S

G

A

D

Y

Y

T

A

Y

Y

A

M

Y

L

A

E

T

V

L

Y

G

G

E

S

F

L

I

P

A

A

W

F

I

L

I

K

G

L

W

W

-

I

D

E

L

L

L

I

E

I

Y

R

A

P

F

S

G

S

A

Q

T

Y

T

I

V

V

A

A

I

L

G

V

W

F

S

A

G

T

Y

Y

V

L

V

-

S

-

F

-

L

L

R

K

D

P

F

L

H

F

I

P

G

T

I

C

P

P

A

V

A

-

L

-

A

-

G

-

A

-

P

-

F

-

A

-

F

-

D

-

P

P

A

E

S

E

G

A

A

A

W

-

T

-

H

-

T

-

G

-

A

-

L

-

V

-

N

K

A

L

P

V

A

A

V

C

A

L

I

C

V

V

L

L

A

L

L

L

T

T

A

A

L

V

L

N

V

C

V

Y

G

S

V

V

N

K

E

A

S

A

A

T

K

R

V

V

N

Q

N

D

I

A

I

F

V

A

A

A

I

A

K

K

L

L

A

L

I

A

I

L

V

A

V

L

F

I

I

I

L

L

A

L

G

G

L

F

S

V

S

Q

V

I

S

G

T

K

A

G

N

D

W

-

V

V

T

S

S

N

A

L

N

D

P

P

D

N

G

F

A

S

F

F

I

E

P

G

P

T

N

K

A

L

G

D

P

V

G

G

E

N

Y

-

G

-

W

-

S

-

G

-

I

I

L

V

R

L

G

A

A

L

A

Y

V

S

V

G

F

L

F
|
F

A

A

Y

Y

I
x
G

G
|
G

F

W

D

N

A

Y

V

L

S

N

T

F

A

V

A

T

Q

E

E

E

A

M

K

I

N

N

P

P

Q

Y

R

R

D

N

M

L

P

P

L

L

G

A

I

I

L

I

G

I

S

S

L

L

V

P

I

I

C

V

T

T

V

L

L

V

Y

Y

V

V

L

L

V

T

S

N

I

L

V

A

I

Y

T

F

G

T

I

T

V

L

P

S

F

T

D

E

R

Q

L

M

S

L

V

S

P

S

D

E

P

A

I

V

A

A

L

V

G

D

V

F

D

G

V

N

I

Y

G

H

L

L

R

G

W

V

L

M

S

S

T

W

V

I

K

P

L

V

G

F

A

V

I

G

L

L

G

S

L

C

S

F

S

G

V

S

V

V

L

N

V

G

L

S

L

L

L

F

G

T

Q

S

T

S

R

R

V

L

L

F

Y

F

S

V

I

G

A

S

R

R

D

E

G

G

L

H

L

L

P

P

P

S

I

I

A

L

A

S

K

M

V

I

H

H

P

P

R

Q

F

L

R

L

T

T

P

P

Y

V

L

P

T

S

T

L

I

V

G

F

T

T

G

C

L

V

I

M

V

T

A

L

V

L

M

Y

A

A

G

F

V

S

L

K

P

D

I

I

G

F

L

S

V

V

G

I

E

N

L

F

V
x
F

S

S

I

F

G

F

T

N

L

W

F

L

A

C

F

V

A

A

I

L

V

A

C

I

A

I

G

G

V

M

L

I

F

W

L

L

R

R

Y

H

T

R

H

K

P

P

Q

E

I

L

H

E

R

R

P

P

F

I

R

K

A

V

-

N

-

L

-

A

-

L

P

P

L

V

I

F

A

F

I

I

V

L

A

A

P

C

L

L

G

F

A

L

A

I

A

A

A

-

V

-

V

V

L

S

M

F

L

W

G

K

L

T

P

P

R

V

D

E

T

C

W

G

I

I

R

G

F

F

A

T

I

I

W

I

L

L

A

S

I

-

G

G

L

L

I

P

L

V

Y

Y

F

F

binding 3,4-dihydroxyphenylalanine: I20 (= I46), G22 (= G48), S23 (≠ A49), G24 (= G50), F209 (= F260), G212 (≠ I263), G213 (= G264), F357 (≠ V408)

8x0wB Overall structure of the lat1-4f2hc bound with leu
25% identity, 91% coverage: 29:480/494 of query aligns to 3:430/464 of 8x0wB

query
sites
8x0wB

L

L

R

Q

R

R

T

N

L

I

S

T

L

L

A

L

S

N

L

G

I

V

S

A

L

I

G

I

I

V

G

G

C
x
T

I
|
I

I

I

G
|
G

A
x
S

G

G

I

I

F

F

V

V

L

T

T

P

G

T

H

G

A

V

A

L

A

K

A

E

Y

A

A

G

G

S

P

P

A

G

I

L

S

A

L

L

S

V

-

V

F

W

V

A

L

A

A

C

G

G

L

V

V

F

C

S

A

I

L

V

A

G

G

A

L

L

C

C

Y

Y

A

A

E

E

M

L

A

G

S

T

T

T

V

I

P

S

V

K

A

S

G

G

S

G

A

D

Y

Y

T

A

Y

Y

A

M

Y

L

A

E

T

V

L

Y

G

G

E

S

F

L

I

P

A

A

W

F

I

L

I

K

G

L

W

W

-

I

D

E

L

L

L

I

E

I

Y

R

A

P

F

S

G

S

A

Q

T

Y

T

I

V

V

A

A

I

L

G

V

W

F

S

A

G

T

Y

Y

V

L

V

-

S

-

F

-

L

L

R

K

D

P

F

L

H

F

I

P

G

T

I

C

P

P

A

V

A

-

L

-

A

-

G

-

A

-

P

-

F

-

A

-

F

-

D

-

P

P

A

E

S

E

G

A

A

A

W

-

T

-

H

-

T

-

G

-

A

-

L

-

V

-

N

K

A

L

P

V

A

A

V

C

A

L

I

C

V

V

L

L

A

L

L

L

T

T

A

A

L

V

L

N

V

C

V

Y

G

S

V

V

N

K

E

A

S

A

A

T

K

R

V

V

N

Q

N

D

I

A

I

F

V

A

A

A

I

A

K

K

L

L

A

L

I

A

I

L

V

A

V

L

F

I

I

I

L

L

A

L

G

G

L

F

S

V

S

Q

V

I

S

G

T

K

A

G

N

D

W

-

V

V

T

S

S

N

A

L

N

D

P

P

D

N

G

F

A

S

F

F

I

E

P

G

P

T

N

K

A

L

G

D

P

V

G

G

E

N

Y

-

G

-

W

-

S

-

G

-

I

I

L

V

R

L

G

A

A

L

A

Y

V

S

V

G

F

L

F

F

A

A

Y

Y

I
x
G

G

G

F

W

D

N

A

Y

V

L

S

N

T

F

A

V

A

T

Q

E

E

E

A

M

K

I

N

N

P

P

Q

Y

R

R

D

N

M

L

P

P

L

L

G

A

I

I

L

I

G

I

S

S

L

L

V

P

I

I

C

V

T

T

V

L

L

V

Y

Y

V

V

L

L

V

T

S

N

I

L

V

A

I

Y

T

F

G

T

I

T

V

L

P

S

F

T

D

E

R

Q

L

M

S

L

V

S

P

S

D

E

P

A

I

V

A

A

L

V

G

D

V

F

D

G

V

N

I

Y

G

H

L

L

R

G

W

V

L

M

S

S

T

W

V

I

K

P

L

V

G

F

A

V

I

G

L

L

G

S

L

C

S

F

S

G

V

S

V

V

L

N

V

G

L

S

L

L

L

F

G

T

Q

S

T

S

R

R

V

L

L

F

Y

F

S

V

I

G

A

S

R

R

D

E

G

G

L

H

L

L

P

P

P

S

I

I

A

L

A

S

K

M

V

I

H

H

P

P

R

Q

F

L

R

L

T

T

P

P

Y

V

L

P

T

S

T

L

I

V

G

F

T

T

G

C

L

V

I

M

V

T

A

L

V

L

M

Y

A

A

G

F

V

S

L

K

P

D

I

I

G

F

L

S

V

V

G

I

E

N

L

F

V

F

S

S

I

F

G

F

T

N

L

W

F

L

A

C

F

V

A

A

I

L

V

A

C

I

A

I

G

G

V

M

L

I

F

W

L

L

R

R

Y

H

T

R

H

K

P

P

Q

E

I

L

H

E

R

R

P

P

F

I

R

K

A

V

-

N

-

L

-

A

-

L

P

P

L

V

I

F

A

F

I

I

V

L

A

A

P

C

L

L

G

F

A

L

A

I

A

A

A

-

V

-

V

V

L

S

M

F

L

W

G

K

L

T

P

P

R

V

D

E

T

C

W

G

I

I

R

G

F

F

A

T

I

I

W

I

L

L

A

S

I

-

G

G

L

L

I

P

L

V

Y

Y

F

F

binding leucine: T19 (≠ C45), I20 (= I46), G22 (= G48), S23 (≠ A49), G212 (≠ I263)

8idaB Overall structure of the lat1-4f2hc bound with tyrosine
25% identity, 91% coverage: 29:480/494 of query aligns to 3:430/464 of 8idaB

query
sites
8idaB

L

L

R

Q

R

R

T

N

L

I

S

T

L

L

A

L

S

N

L

G

I

V

S

A

L

I

G

I

I

V

G

G

C

T

I
|
I

I

I

G
|
G

A

S

G
|
G

I

I

F

F

V

V

L

T

T

P

G

T

H

G

A

V

A

L

A

K

A

E

Y

A

A

G

G

S

P

P

A

G

I

L

S

A

L

L

S

V

-

V

F

W

V

A

L

A

A

C

G

G

L

V

V

F

C

S

A

I

L

V

A

G

G

A

L

L

C

C

Y

Y

A

A

E

E

M

L

A

G

S

T

T

T

V

I

P

S

V

K

A

S

G

G

S

G

A

D

Y

Y

T

A

Y

Y

A

M

Y

L

A

E

T

V

L

Y

G

G

E

S

F

L

I

P

A

A

W

F

I

L

I

K

G

L

W

W

-

I

D

E

L

L

L

I

E

I

Y

R

A

P

F

S

G

S

A

Q

T

Y

T

I

V

V

A

A

I

L

G

V

W

F

S

A

G

T

Y

Y

V

L

V

-

S

-

F

-

L

L

R

K

D

P

F

L

H

F

I

P

G

T

I

C

P

P

A

V

A

-

L

-

A

-

G

-

A

-

P

-

F

-

A

-

F

-

D

-

P

P

A

E

S

E

G

A

A

A

W

-

T

-

H

-

T

-

G

-

A

-

L

-

V

-

N

K

A

L

P

V

A

A

V

C

A

L

I

C

V

V

L

L

A

L

L

L

T

T

A

A

L

V

L

N

V

C

V

Y

G

S

V

V

N

K

E

A

S

A

A

T

K

R

V

V

N

Q

N

D

I

A

I

F

V

A

A

A

I

A

K

K

L

L

A

L

I

A

I

L

V

A

V

L

F

I

I

I

L

L

A

L

G

G

L

F

S

V

S

Q

V

I

S

G

T

K

A

G

N

D

W

-

V

V

T

S

S

N

A

L

N

D

P

P

D

N

G

F

A

S

F

F

I

E

P

G

P

T

N

K

A

L

G

D

P

V

G

G

E

N

Y

-

G

-

W

-

S

-

G

-

I

I

L

V

R

L

G

A

A

L

A

Y

V

S

V

G

F

L

F

F

A

A

Y

Y

I
x
G

G

G

F

W

D

N

A
x
Y

V

L

S

N

T

F

A

V

A

T

Q

E

E

E

A

M

K

I

N

N

P

P

Q

Y

R

R

D

N

M

L

P

P

L

L

G

A

I

I

L

I

G

I

S

S

L

L

V

P

I

I

C

V

T

T

V

L

L

V

Y

Y

V

V

L

L

V

T

S

N

I

L

V

A

I

Y

T

F

G

T

I

T

V

L

P

S

F

T

D

E

R

Q

L

M

S

L

V

S

P

S

D

E

P

A

I

V

A

A

L

V

G

D

V

F

D

G

V

N

I

Y

G

H

L

L

R

G

W

V

L

M

S

S

T

W

V

I

K

P

L

V

G

F

A

V

I

G

L

L

G

S

L

C

S

F

S

G

V

S

V

V

L

N

V

G

L

S

L

L

L

F

G

T

Q

S

T

S

R

R

V

L

L

F

Y

F

S

V

I

G

A

S

R

R

D

E

G

G

L

H

L

L

P

P

P

S

I

I

A

L

A

S

K

M

V

I

H

H

P

P

R

Q

F

L

R

L

T

T

P

P

Y

V

L

P

T

S

T

L

I

V

G

F

T

T

G

C

L

V

I

M

V

T

A

L

V

L

M

Y

A

A

G

F

V

S

L

K

P

D

I

I

G

F

L

S

V

V

G

I

E

N

L

F

V

F

S

S

I

F

G

F

T

N

L

W

F

L

A

C

F

V

A

A

I

L

V

A

C

I

A

I

G

G

V

M

L

I

F

W

L

L

R

R

Y

H

T

R

H

K

P

P

Q

E

I

L

H

E

R

R

P

P

F

I

R

K

A

V

-

N

-

L

-

A

-

L

P

P

L

V

I

F

A

F

I

I

V

L

A

A

P

C

L

L

G

F

A

L

A

I

A

A

A

-

V

-

V

V

L

S

M

F

L

W

G

K

L

T

P

P

R

V

D

E

T

C

W

G

I

I

R

G

F

F

A

T

I

I

W

I

L

L

A

S

I

-

G

G

L

L

I

P

L

V

Y

Y

F

F

binding tyrosine: I20 (= I46), G22 (= G48), G24 (= G50), G212 (≠ I263), Y216 (≠ A267)

7dsqB Overall structure of the lat1-4f2hc bound with 3,5-diiodo-l-tyrosine (see paper)
25% identity, 91% coverage: 29:480/494 of query aligns to 3:430/464 of 7dsqB

query
sites
7dsqB

L

L

R

Q

R

R

T

N

L

I

S

T

L

L

A

L

S

N

L

G

I

V

S

A

L

I

G

I

I

V

G

G

C
x
T

I
|
I

I

I

G
|
G

A

S

G

G

I

I

F

F

V

V

L

T

T

P

G

T

H

G

A

V

A

L

A

K

A

E

Y

A

A

G

G

S

P

P

A

G

I

L

S

A

L

L

S

V

-

V

F

W

V

A

L

A

A

C

G

G

L

V

V

F

C

S

A

I

L

V

A

G

G

A

L

L

C

C

Y

Y

A

A

E

E

M

L

A

G

S

T

T

T

V

I

P

S

V

K

A

S

G

G

S

G

A

D

Y

Y

T

A

Y

Y

A

M

Y

L

A

E

T

V

L

Y

G

G

E

S

F

L

I

P

A

A

W

F

I

L

I

K

G

L

W

W

-

I

D

E

L

L

L

I

E

I

Y

R

A

P

F

S

G

S

A

Q

T

Y

T

I

V

V

A

A

I

L

G

V

W

F

S

A

G

T

Y

Y

V

L

V

-

S

-

F

-

L

L

R

K

D

P

F

L

H

F

I

P

G

T

I

C

P

P

A

V

A

-

L

-

A

-

G

-

A

-

P

-

F

-

A

-

F

-

D

-

P

P

A

E

S

E

G

A

A

A

W

-

T

-

H

-

T

-

G

-

A

-

L

-

V

-

N

K

A

L

P

V

A

A

V

C

A

L

I

C

V

V

L

L

A

L

L

L

T

T

A

A

L

V

L

N

V

C

V

Y

G

S

V

V

N
x
K

E

A

S

A

A

T

K

R

V
|
V

N

Q

N

D

I

A

I

F

V

A

A

A

I

A

K

K

L

L

A

L

I

A

I

L

V

A

V

L

F

I

I

I

L

L

A

L

G

G

L

F

S

V

S

Q

V

I

S

G

T

K

A

G

N

D

W

-

V

V

T

S

S

N

A

L

N

D

P

P

D

N

G

F

A

S

F

F

I

E

P

G

P

T

N

K

A

L

G

D

P

V

G

G

E

N

Y

-

G

-

W

-

S

-

G

-

I

I

L

V

R

L

G

A

A

L

A

Y

V

S

V

G

F

L

F

F

A

A

Y

Y

I
x
G

G
|
G

F

W

D

N

A
x
Y

V

L

S

N

T

F

A

V

A

T

Q

E

E

E

A

M

K

I

N

N

P

P

Q

Y

R

R

D

N

M

L

P

P

L

L

G

A

I

I

L

I

G

I

S

S

L

L

V

P

I

I

C

V

T

T

V

L

L

V

Y

Y

V

V

L

L

V

T

S

N

I

L

V

A

I

Y

T

F

G

T

I

T

V

L

P

S

F

T

D

E

R

Q

L

M

S

L

V

S

P

S

D

E

P

A

I

V

A

A

L

V

G

D

V

F

D

G

V

N

I

Y

G

H

L

L

R

G

W

V

L

M

S

S

T

W

V

I

K

P

L

V

G

F

A

V

I

G

L

L

G

S

L

C

S

F

S

G

V

S

V

V

L

N

V

G

L

S

L

L

L

F

G

T

Q

S

T

S

R

R

V

L

L

F

Y

F

S

V

I

G

A

S

R

R

D

E

G

G

L

H

L

L

P

P

P

S

I

I

A

L

A

S

K

M

V

I

H

H

P

P

R

Q

F

L

R

L

T

T

P

P

Y

V

L

P

T

S

T

L

I

V

G

F

T

T

G

C

L

V

I

M

V

T

A

L

V

L

M

Y

A

A

G

F

V

S

L

K

P

D

I

I

G

F

L

S

V

V

G

I

E

N

L

F

V
x
F

S

S

I

F

G

F

T
x
N

L

W

F

L

A

C

F

V

A

A

I

L

V

A

C

I

A

I

G

G

V

M

L

I

F

W

L

L

R

R

Y

H

T

R

H

K

P

P

Q

E

I

L

H

E

R

R

P

P

F

I

R

K

A

V

-

N

-

L

-

A

-

L

P

P

L

V

I

F

A

F

I

I

V

L

A

A

P

C

L

L

G

F

A

L

A

I

A

A

A

-

V

-

V

V

L

S

M

F

L

W

G

K

L

T

P

P

R

V

D

E

T

C

W

G

I

I

R

G

F

F

A

T

I

I

W

I

L

L

A

S

I

-

G

G

L

L

I

P

L

V

Y

Y

F

F

binding 3,5-diiodotyrosine: T19 (≠ C45), I20 (= I46), G22 (= G48), G212 (≠ I263), G213 (= G264), Y216 (≠ A267), F357 (≠ V408), N361 (≠ T412)
binding cholesterol hemisuccinate: K148 (≠ N193), V153 (= V198)

7dsnB Overall structure of the lat1-4f2hc bound with jx-119 (see paper)
25% identity, 91% coverage: 29:480/494 of query aligns to 3:430/464 of 7dsnB

query
sites
7dsnB

L

L

R

Q

R

R

T

N

L

I

S

T

L

L

A

L

S

N

L

G

I

V

S

A

L

I

G

I

I

V

G

G

C
x
T

I
|
I

I

I

G
|
G

A
x
S

G
|
G

I

I

F

F

V

V

L

T

T

P

G

T

H

G

A

V

A

L

A

K

A

E

Y

A

A

G

G

S

P

P

A

G

I

L

S

A

L

L

S

V

-

V

F

W

V

A

L

A

A

C

G

G

L

V

V

F

C

S

A

I

L

V

A

G

G

A

L

L

C

C

Y

Y

A

A

E

E

M

L

A

G

S

T

T

T

V

I

P

S

V

K

A

S

G

G

S

G

A

D

Y

Y

T

A

Y

Y

A

M

Y

L

A

E

T

V

L

Y

G

G

E

S

F

L

I

P

A

A

W

F

I

L

I

K

G

L

W

W

-

I

D

E

L

L

L

I

E
x
I

Y

R

A

P

F

S

G

S

A

Q

T

Y

T
x
I

V

V

A

A

I

L

G

V

W
x
F

S

A

G

T

Y

Y

V

L

V

-

S

-

F

-

L

L

R

K

D

P

F

L

H

F

I

P

G

T

I

C

P

P

A

V

A

-

L

-

A

-

G

-

A

-

P

-

F

-

A

-

F

-

D

-

P

P

A

E

S

E

G

A

A

A

W

-

T

-

H

-

T

-

G

-

A

-

L

-

V

-

N

K

A

L

P

V

A

A

V

C

A

L

I

C

V

V

L

L

A

L

L

L

T

T

A

A

L

V

L

N

V

C

V
x
Y

G

S

V

V

N
x
K

E

A

S

A

A

T

K

R

V
|
V

N

Q

N

D

I

A

I

F

V

A

A

A

I

A

K

K

L

L

A

L

I

A

I

L

V

A

V

L

F

I

I

I

L

L

A

L

G

G

L

F

S

V

S

Q

V

I

S

G

T

K

A

G

N

D

W

-

V

V

T

S

S

N

A

L

N

D

P

P

D

N

G

F

A

S

F

F

I

E

P

G

P

T

N

K

A

L

G

D

P

V

G

G

E

N

Y

-

G

-

W

-

S

-

G

-

I

I

L

V

R

L

G

A

A

L

A

Y

V

S

V

G

F

L

F
|
F

A
|
A

Y

Y

I
x
G

G

G

F

W

D

N

A

Y

V

L

S

N

T

F

A

V

A

T

Q

E

E

E

A

M

K

I

N

N

P

P

Q

Y

R

R

D

N

M

L

P

P

L

L

G

A

I

I

L

I

G

I

S

S

L

L

V

P

I

I

C

V

T

T

V

L

L

V

Y

Y

V

V

L

L

V

T

S

N

I

L

V

A

I

Y

T

F

G

T

I

T

V

L

P

S

F

T

D

E

R

Q

L

M

S

L

V

S

P

S

D

E

P

A

I

V

A

A

L

V

G

D

V

F

D

G

V

N

I

Y

G

H

L

L

R

G

W

V

L

M

S

S

T

W

V

I

K

P

L

V

G

F

A

V

I

G

L

L

G

S

L

C

S

F

S

G

V

S

V

V

L

N

V

G

L

S

L

L

L

F

G

T

Q

S

T

S

R

R

V

L

L

F

Y

F

S

V

I

G

A

S

R

R

D

E

G

G

L

H

L

L

P

P

P

S

I

I

A

L

A

S

K

M

V

I

H

H

P

P

R
x
Q

F

L

R

L

T

T

P

P

Y

V

L

P

T

S

T

L

I

V

G

F

T

T

G

C

L

V

I

M

V

T

A

L

V

L

M

Y

A

A

G

F

V

S

L

K

P

D

I

I

G

F

L

S

V

V

G
x
I

E

N

L

F

V

F

S

S

I

F

G

F

T
x
N

L

W

F

L

A

C

F

V

A

A

I

L

V

A

C

I

A

I

G

G

V

M

L

I

F

W

L

L

R

R

Y

H

T

R

H

K

P

P

Q

E

I

L

H

E

R

R

P

P

F

I

R

K

A

V

-

N

-

L

-

A

-

L

P

P

L

V

I

F

A

F

I

I

V

L

A

A

P

C

L

L

G

F

A

L

A

I

A

A

A

-

V

-

V

V

L

S

M

F

L

W

G

K

L

T

P

P

R

V

D

E

T

C

W

G

I

I

R

G

F

F

A

T

I

I

W

I

L

L

A

S

I

-

G

G

L

L

I

P

L

V

Y

Y

F

F

binding (2~{S})-2-azanyl-7-[[2-(1,3-benzoxazol-2-yl)phenyl]methoxy]-3,4-dihydro-1~{H}-naphthalene-2-carboxylic acid: T19 (≠ C45), I20 (= I46), G22 (= G48), S23 (≠ A49), G24 (= G50), I97 (≠ E121), I104 (≠ T128), F209 (= F260), A210 (= A261), G212 (≠ I263), I354 (≠ G405), N361 (≠ T412)
binding cholesterol hemisuccinate: F109 (≠ W133), Y145 (≠ V190), K148 (≠ N193), V153 (= V198), Q326 (≠ R377)

Sites not aligning to the query:

binding cholesterol hemisuccinate: 457

7dslB Overall structure of the lat1-4f2hc bound with jx-078 (see paper)
25% identity, 91% coverage: 29:480/494 of query aligns to 3:430/464 of 7dslB

query
sites
7dslB

L

L

R

Q

R

R

T

N

L

I

S

T

L

L

A

L

S

N

L

G

I

V

S

A

L

I

G

I

I

V

G

G

C
x
T

I

I

G

G

A
x
S

G
|
G

I

I

F

F

V

V

L

T

T

P

G

T

H

G

A

V

A

L

A

K

A

E

Y

A

A

G

G

S

P

P

A

G

I

L

S

A

L

L

S

V

-

V

F

W

V

A

L

A

A

C

G

G

L

V

V

F

C

S

A

I

L

V

A

G

G

A

L

L

C

C

Y

Y

A

A

E

E

M

L

A

G

S

T

T

T

V

I

P

S

V

K

A

S

G

G

S

G

A

D

Y

Y

T

A

Y

Y

A

M

Y

L

A

E

T

V

L

Y

G

G

E

S

F

L

I

P

A

A

W

F

I

L

I

K

G

L

W

W

-

I

D

E

L

L

L

I

E

I

Y

R

A

P

F

S

G

S

A

Q

T

Y

T

I

V

V

A

A

I

L

G

V

W

F

S

A

G

T

Y

Y

V

L

V

-

S

-

F

-

L

L

R

K

D

P

F

L

H

F

I

P

G

T

I

C

P

P

A

V

A

-

L

-

A

-

G

-

A

-

P

-

F

-

A

-

F

-

D

-

P

P

A

E

S

E

G

A

A

A

W

-

T

-

H

-

T

-

G

-

A

-

L

-

V

-

N

K

A

L

P

V

A

A

V

C

A

L

I

C

V

V

L

L

A

L

L

L

T

T

A

A

L

V

L

N

V

C

V

Y

G

S

V

V

N

K

E

A

S

A

A

T

K

R

V

V

N

Q

N

D

I

A

I

F

V

A

A

A

I

A

K

K

L

L

A

L

I

A

I

L

V

A

V

L

F

I

I

I

L

L

A

L

G

G

L

F

S

V

S

Q

V

I

S

G

T

K

A

G

N

D

W

-

V

V

T

S

S

N

A

L

N

D

P

P

D

N

G

F

A

S

F

F

I

E

P

G

P

T

N

K

A

L

G

D

P

V

G

G

E

N

Y

-

G

-

W

-

S

-

G

-

I

I

L

V

R

L

G

A

A

L

A

Y

V

S

V

G

F

L

F
|
F

A

A

Y

Y

I
x
G

G

G

F

W

D

N

A
x
Y

V

L

S

N

T

F

A

V

A

T

Q

E

E

E

A

M

K

I

N

N

P

P

Q

Y

R

R

D

N

M

L

P

P

L

L

G

A

I

I

L

I

G

I

S

S

L

L

V

P

I

I

C

V

T

T

V

L

L

V

Y

Y

V

V

L

L

V

T

S

N

I

L

V

A

I

Y

T

F

G

T

I

T

V

L

P

S

F

T

D

E

R

Q

L

M

S

L

V

S

P

S

D

E

P

A

I

V

A

A

L

V

G

D

V

F

D

G

V

N

I

Y

G

H

L

L

R

G

W

V

L

M

S

S

T

W

V

I

K

P

L

V

G

F

A

V

I

G

L

L

G

S

L

C

S

F

S

G

V

S

V

V

L

N

V

G

L

S

L

L

L

F

G

T

Q

S

T

S

R

R

V

L

L

F

Y

F

S

V

I

G

A

S

R

R

D

E

G

G

L

H

L

L

P

P

P

S

I

I

A

L

A

S

K

M

V

I

H

H

P

P

R

Q

F

L

R

L

T

T

P

P

Y

V

L

P

T

S

T

L

I

V

G

F

T

T

G

C

L

V

I

M

V

T

A

L

V

L

M

Y

A

A

G

F

V

S

L

K

P

D

I

I

G

F

L

S

V

V

G
x
I

E

N

L

F

V

F

S

S

I

F

G

F

T

N

L

W

F

L

A

C

F

V

A

A

I

L

V

A

C

I

A

I

G

G

V

M

L

I

F

W

L

L

R

R

Y

H

T

R

H

K

P

P

Q

E

I

L

H

E

R

R

P

P

F

I

R

K

A

V

-

N

-

L

-

A

-

L

P

P

L

V

I

F

A

F

I

I

V

L

A

A

P

C

L

L

G

F

A

L

A

I

A

A

A

-

V

-

V

V

L

S

M

F

L

W

G

K

L

T

P

P

R

V

D

E

T

C

W

G

I

I

R

G

F

F

A

T

I

I

W

I

L

L

A

S

I

-

G

G

L

L

I

P

L

V

Y

Y

F

F

binding (2~{S})-2-azanyl-7-[(2-phenylphenyl)methoxy]-3,4-dihydro-1~{H}-naphthalene-2-carboxylic acid: T19 (≠ C45), S23 (≠ A49), G24 (= G50), F209 (= F260), G212 (≠ I263), Y216 (≠ A267), I354 (≠ G405)

7dskB Overall structure of the lat1-4f2hc bound with jx-075 (see paper)
25% identity, 91% coverage: 29:480/494 of query aligns to 3:430/464 of 7dskB

query
sites
7dskB

L

L

R

Q

R

R

T

N

L

I

S

T

L

L

A

L

S

N

L

G

I

V

S

A

L

I

G

I

I

V

G

G

C
x
T

I
|
I

I

I

G

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F

binding (2~{S})-2-azanyl-7-(naphthalen-1-ylmethoxy)-3,4-dihydro-1~{H}-naphthalene-2-carboxylic acid: T19 (≠ C45), I20 (= I46), S23 (≠ A49), G24 (= G50), I97 (≠ E121), S100 (≠ F124), S101 (≠ G125), F209 (= F260), G212 (≠ I263), Y216 (≠ A267), V353 (= V404), I354 (≠ G405), N361 (≠ T412)
binding cholesterol hemisuccinate: K148 (≠ N193), A149 (≠ E194), V153 (= V198), F157 (≠ I202), H324 (= H375)

Sites not aligning to the query:

binding cholesterol hemisuccinate: 457

7b00A Human lat2-4f2hc complex in the apo-state (see paper)
25% identity, 83% coverage: 35:442/494 of query aligns to 2:385/457 of 7b00A

query
sites
7b00A

L

L

A

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M

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binding Digitonin: M338 (≠ I391), Y350 (≠ L403)

Sites not aligning to the query:

binding Digitonin: 408, 411

Query Sequence

>WP_012589784.1 NCBI__GCF_000021745.1:WP_012589784.1
MTSIWSTKSIASLKAEAHEAEFGSETPNLRRTLSLASLISLGIGCIIGAGIFVLTGHAAA
AYAGPAISLSFVLAGLVCALAGLCYAEMASTVPVAGSAYTYAYATLGEFIAWIIGWDLLL
EYAFGATTVAIGWSGYVVSFLRDFHIGIPAALAGAPFAFDPASGAWTHTGALVNAPAVAI
VLALTALLVVGVNESAKVNNIIVAIKLAIIVVFILAGLSSVSTANWVTSANPDGAFIPPN
AGPGEYGWSGILRGAAVVFFAYIGFDAVSTAAQEAKNPQRDMPLGILGSLVICTVLYVLV
SIVITGIVPFDRLSVPDPIALGVDVIGLRWLSTVVKLGAILGLSSVVLVLLLGQTRVLYS
IARDGLLPPIAAKVHPRFRTPYLTTIGTGLIVAVMAGVLPIGLVGELVSIGTLFAFAIVC
AGVLFLRYTHPQIHRPFRAPLIAIVAPLGAAAAVVLMLGLPRDTWIRFAIWLAIGLILYF
TYGRRHSRLARAAE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory