SitesBLAST
Comparing WP_012590207.1 NCBI__GCF_000021745.1:WP_012590207.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
5u4qA 1.5 angstrom resolution crystal structure of NAD-dependent epimerase from klebsiella pneumoniae in complex with NAD.
51% identity, 99% coverage: 1:333/337 of query aligns to 2:318/321 of 5u4qA
- binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G10), F12 (= F11), I13 (= I12), D32 (= D31), N33 (≠ G32), N35 (≠ T34), Y38 (= Y37), K43 (= K42), D61 (≠ L61), L62 (= L62), L83 (≠ F82), A84 (= A83), A85 (= A84), A118 (= A124), Y145 (= Y151), K149 (= K155), F172 (= F178), F173 (= F179), T174 (= T180), V175 (= V181), R181 (= R187)
5u4qB 1.5 angstrom resolution crystal structure of NAD-dependent epimerase from klebsiella pneumoniae in complex with NAD.
49% identity, 99% coverage: 1:333/337 of query aligns to 2:302/304 of 5u4qB
- binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G10), F12 (= F11), I13 (= I12), D32 (= D31), N33 (≠ G32), N35 (≠ T34), Y38 (= Y37), K43 (= K42), D61 (≠ L61), L62 (= L62), L83 (≠ F82), A84 (= A83), A85 (= A84), A123 (= A124), Y150 (= Y151), K154 (= K155), F177 (= F178), V180 (= V181), R186 (= R187), M189 (= M190)
6zldA Crystal structure of udp-glucuronic acid 4-epimerase from bacillus cereus in complex with udp-glucuronic acid and NAD (see paper)
32% identity, 99% coverage: 1:332/337 of query aligns to 1:312/314 of 6zldA
- active site: T126 (= T126), S127 (= S127), S128 (= S128), Y149 (= Y151), K153 (= K155)
- binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (= I12), D32 (= D31), H33 (≠ G32), F34 (= F33), I35 (≠ T34), K43 (= K42), D62 (≠ A59), I63 (= I60), L81 (≠ F82), A82 (= A83), A83 (= A84), I124 (≠ A124), T126 (= T126), K153 (= K155), Y176 (≠ F178), T178 (= T180), R185 (= R187), M188 (= M190)
- binding uridine-5'-diphosphate-glucuronic acid: P85 (≠ A86), R88 (= R89), T126 (= T126), S127 (= S127), S128 (= S128), Y149 (= Y151), F177 (= F179), T178 (= T180), R185 (= R187), M188 (= M190), A189 (= A191), R192 (≠ K194), T204 (≠ D206), F206 (= F208), Q211 (≠ M213), R213 (= R215), I250 (≠ L270), E276 (≠ D296)
6zl6A Crystal structure of udp-glucuronic acid 4-epimerase from bacillus cereus in complex with udp and NAD (see paper)
32% identity, 99% coverage: 1:332/337 of query aligns to 1:312/314 of 6zl6A
- active site: T126 (= T126), S127 (= S127), S128 (= S128), Y149 (= Y151), K153 (= K155)
- binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (= I12), D32 (= D31), H33 (≠ G32), F34 (= F33), I35 (≠ T34), K43 (= K42), D62 (≠ A59), I63 (= I60), L81 (≠ F82), A82 (= A83), A83 (= A84), I124 (≠ A124), T126 (= T126), K153 (= K155), Y176 (≠ F178), T178 (= T180), V179 (= V181), R185 (= R187), M188 (= M190)
- binding uridine-5'-diphosphate: T178 (= T180), A189 (= A191), R192 (≠ K194), T204 (≠ D206), F206 (= F208), Q211 (≠ M213), R213 (= R215), I250 (≠ L270), E276 (≠ D296)
6zllA Crystal structure of udp-glucuronic acid 4-epimerase from bacillus cereus in complex with udp-galacturonic acid and NAD (see paper)
32% identity, 99% coverage: 1:332/337 of query aligns to 1:312/321 of 6zllA
- active site: T126 (= T126), S127 (= S127), S128 (= S128), Y149 (= Y151), K153 (= K155)
- binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (= I12), D32 (= D31), H33 (≠ G32), F34 (= F33), I35 (≠ T34), K43 (= K42), D62 (≠ A59), I63 (= I60), L81 (≠ F82), A82 (= A83), A83 (= A84), I124 (≠ A124), T126 (= T126), Y149 (= Y151), K153 (= K155), Y176 (≠ F178), V179 (= V181), R185 (= R187), M188 (= M190)
- binding (2S,3R,4S,5R,6R)-6-[[[(2R,3S,4R,5R)-5-(2,4-dioxopyrimidin-1-yl)-3,4-dihydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl]oxy-hydroxy-phosphoryl]oxy-3,4,5-trihydroxy-oxane-2-carboxylic acid: P85 (≠ A86), V87 (= V88), R88 (= R89), T126 (= T126), S127 (= S127), Y149 (= Y151), T178 (= T180), R185 (= R187), A189 (= A191), R192 (≠ K194), T204 (≠ D206), F206 (= F208), Q211 (≠ M213), R213 (= R215), I250 (≠ L270), E276 (≠ D296)
6zljA Crystal structure of udp-glucuronic acid 4-epimerase y149f mutant from bacillus cereus in complex with udp-4-deoxy-4-fluoro-glucuronic acid and NAD (see paper)
32% identity, 99% coverage: 1:332/337 of query aligns to 1:312/314 of 6zljA
- active site: T126 (= T126), S127 (= S127), S128 (= S128), F149 (≠ Y151), K153 (= K155)
- binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (= I12), D32 (= D31), H33 (≠ G32), F34 (= F33), I35 (≠ T34), K43 (= K42), D62 (≠ A59), I63 (= I60), L81 (≠ F82), A82 (= A83), A83 (= A84), I124 (≠ A124), T126 (= T126), K153 (= K155), Y176 (≠ F178), T178 (= T180), V179 (= V181), R185 (= R187), M188 (= M190)
- binding (2~{R},3~{S},4~{R},5~{R},6~{R})-6-[[[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3-fluoranyl-4,5-bis(oxidanyl)oxane-2-carboxylic acid: P85 (≠ A86), R88 (= R89), T126 (= T126), S127 (= S127), S128 (= S128), F149 (≠ Y151), F177 (= F179), T178 (= T180), R185 (= R187), M188 (= M190), A189 (= A191), R192 (≠ K194), T204 (≠ D206), F206 (= F208), Q211 (≠ M213), R213 (= R215), I250 (≠ L270), E276 (≠ D296)
6kv9A Moee5 in complex with udp-glucuronic acid and NAD (see paper)
32% identity, 99% coverage: 3:334/337 of query aligns to 3:299/299 of 6kv9A
- active site: S110 (≠ T126), S111 (= S127), S112 (= S128), Y133 (= Y151), K137 (= K155)
- binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (= I12), D31 (= D31), R32 (≠ G32), R33 (≠ F33), D47 (≠ A47), L48 (≠ I48), L66 (≠ F82), A67 (= A83), A68 (= A84), P70 (≠ A86), C85 (≠ A101), A108 (= A124), S109 (= S125), K137 (= K155), F162 (= F178), T164 (= T180), V165 (= V181), R171 (= R187), M174 (= M190)
- binding uridine-5'-diphosphate-glucuronic acid: P70 (≠ A86), R73 (= R89), S110 (≠ T126), S111 (= S127), Y133 (= Y151), T164 (= T180), R171 (= R187), M174 (= M190), F175 (≠ A191), R178 (≠ K194), E190 (≠ D206), I191 (≠ V207), Y192 (≠ F208), Q197 (≠ M213), R199 (= R215), V235 (≠ L270), D261 (= D296)
4zrnA Crystal structure of udp-glucose 4-epimerase (tm0509) with udp-glucose from hyperthermophilic eubacterium thermotoga maritima (see paper)
29% identity, 99% coverage: 1:334/337 of query aligns to 1:308/309 of 4zrnA
- active site: T117 (= T126), G119 (≠ S127), A120 (≠ S128), Y143 (= Y151), K147 (= K155), Y181 (≠ W185), G185 (≠ D189)
- binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (= I12), D31 (= D31), N32 (≠ G32), S34 (≠ K42), S35 (≠ R43), G36 (≠ R44), S51 (≠ A59), I52 (= I60), L73 (≠ F82), A74 (= A83), A75 (= A84), T92 (≠ A101), S115 (≠ A124), S116 (= S125), Y143 (= Y151), K147 (= K155), Y170 (≠ F178), V173 (= V181)
- binding uridine-5'-diphosphate-glucose: T117 (= T126), G119 (≠ S127), A120 (≠ S128), Y143 (= Y151), N172 (≠ T180), G185 (≠ D189), V186 (≠ M190), H201 (≠ D206), F203 (= F208), Y208 (≠ M213), R210 (= R215), V244 (≠ L270), R267 (≠ Q293), D270 (= D296)
6kvcA Moee5 in complex with udp-glucose and NAD (see paper)
32% identity, 99% coverage: 3:335/337 of query aligns to 3:299/299 of 6kvcA
- active site: S109 (≠ T126), S110 (= S127), S111 (= S128), Y132 (= Y151), K136 (= K155)
- binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (= I12), D31 (= D31), R32 (≠ F33), R33 (≠ T34), D46 (≠ A47), L47 (≠ I48), L65 (≠ F82), A66 (= A83), A67 (= A84), P69 (≠ A86), A107 (= A124), S109 (≠ T126), K136 (= K155), F161 (= F178), T163 (= T180), V164 (= V181), R170 (= R187), M173 (= M190)
- binding uridine-5'-diphosphate-glucose: P69 (≠ A86), R72 (= R89), S109 (≠ T126), S110 (= S127), Y132 (= Y151), T163 (= T180), M173 (= M190), F174 (≠ A191), R177 (≠ K194), E189 (≠ D206), I190 (≠ V207), Y191 (≠ F208), Q196 (≠ M213), R198 (= R215), D260 (= D296)
6wj9B Udp-glcnac c4-epimerase mutant s121a/y146f from pseudomonas protegens in complex with udp-glcnac (see paper)
30% identity, 96% coverage: 2:324/337 of query aligns to 3:302/308 of 6wj9B
- active site: A119 (≠ T126), A120 (≠ S127), A121 (≠ S128), F144 (≠ Y151), K148 (= K155)
- binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G10), F12 (= F11), I13 (= I12), D32 (= D31), D33 (≠ E40), S35 (≠ K42), T36 (≠ R43), G37 (≠ R44), D55 (≠ L62), A56 (≠ E63), L75 (≠ F82), A76 (= A83), A77 (= A84), S94 (≠ A101), A117 (= A124), A119 (≠ T126), F144 (≠ Y151), K148 (= K155), F171 (= F178), F172 (= F179), I174 (≠ V181)
- binding uridine-diphosphate-n-acetylglucosamine: V81 (= V88), N173 (≠ T180), G187 (≠ K194), V188 (≠ F195), F192 (≠ M199), T203 (≠ D206), L204 (≠ V207), F205 (= F208), R212 (= R215), L248 (= L270), R271 (≠ Q293), D274 (= D296)
6wjaA Udp-glcnac c4-epimerase mutant s121a/y146f from pseudomonas protegens in complex with udp-galnac (see paper)
30% identity, 96% coverage: 2:324/337 of query aligns to 2:301/307 of 6wjaA
- active site: A118 (≠ T126), A119 (≠ S127), A120 (≠ S128), F143 (≠ Y151), K147 (= K155)
- binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (= I12), D31 (= D31), D32 (≠ E40), S34 (≠ K42), T35 (≠ R43), G36 (≠ R44), A55 (≠ E63), L74 (≠ F82), A75 (= A83), A76 (= A84), S93 (≠ A101), F143 (≠ Y151), K147 (= K155), F170 (= F178), F171 (= F179), I173 (≠ V181)
- binding uridine-diphosphate-n-acetylgalactosamine: V80 (= V88), A120 (≠ S128), N172 (≠ T180), G186 (≠ K194), V187 (≠ F195), F191 (≠ M199), T202 (≠ D206), F204 (= F208), R211 (= R215), L247 (= L270), R270 (≠ Q293), D273 (= D296)
1hzjA Human udp-galactose 4-epimerase: accommodation of udp-n- acetylglucosamine within the active site (see paper)
28% identity, 98% coverage: 2:332/337 of query aligns to 3:338/345 of 1hzjA
- active site: S131 (≠ T126), A132 (≠ S127), T133 (≠ S128), Y156 (= Y151), K160 (= K155)
- binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G10), Y12 (≠ F11), I13 (= I12), D32 (= D31), N33 (≠ G32), H35 (≠ T34), N36 (≠ H35), D65 (≠ A59), I66 (= I60), F87 (= F82), A88 (= A83), G89 (≠ A84), K91 (≠ A86), S129 (≠ A124), S131 (≠ T126), Y156 (= Y151), K160 (= K155), Y184 (≠ F178), P187 (≠ V181)
- binding uridine-diphosphate-n-acetylglucosamine: N186 (≠ T180), N206 (≠ A191), L207 (= L192), N223 (≠ D206), V224 (= V207), F225 (= F208), R238 (= R215), Y240 (≠ F217), V276 (≠ L270), R299 (≠ Q293), D302 (= D296)
2b69A Crystal structure of human udp-glucoronic acid decarboxylase
27% identity, 100% coverage: 2:337/337 of query aligns to 3:311/312 of 2b69A
- active site: T115 (= T126), S116 (= S127), E117 (≠ S128), Y144 (= Y151), K148 (= K155), R185 (vs. gap)
- binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G10), F12 (= F11), V13 (≠ I12), D32 (= D31), N33 (≠ G32), T36 (≠ L41), G37 (≠ K42), D57 (≠ A59), V58 (≠ I60), L72 (≠ F82), A73 (= A83), S74 (≠ A84), A76 (= A86), T91 (≠ A101), T115 (= T126), Y144 (= Y151), K148 (= K155), I171 (≠ F178), N173 (≠ T180), R185 (vs. gap)
- binding uridine-5'-diphosphate: P61 (≠ R70), L62 (≠ V71), Y63 (= Y72), P78 (≠ V88), N98 (= N108), G101 (≠ E111), L102 (= L112), K104 (≠ R114), R105 (≠ E115), Y158 (= Y165), N173 (≠ T180), R185 (vs. gap), V186 (≠ A191), N189 (≠ K194), T201 (≠ D206), Y203 (≠ F208), Q208 (≠ M213), R210 (= R215), I244 (≠ L270), D270 (= D296)
Q8NBZ7 UDP-glucuronic acid decarboxylase 1; UDP-glucuronate decarboxylase 1; UGD; UXS-1; hUXS; hUXS1; EC 4.1.1.35 from Homo sapiens (Human) (see 4 papers)
27% identity, 100% coverage: 2:337/337 of query aligns to 90:398/420 of Q8NBZ7
- G98 (= G10) binding NAD(+)
- F99 (= F11) binding NAD(+)
- V100 (≠ I12) binding NAD(+)
- D119 (= D31) binding NAD(+)
- N120 (≠ G32) binding NAD(+)
- F122 (≠ T34) binding NAD(+)
- T123 (≠ L41) binding NAD(+)
- G124 (≠ K42) binding NAD(+)
- D144 (≠ A59) binding NAD(+)
- V145 (≠ I60) binding NAD(+)
- L149 (≠ V71) binding UDP-alpha-D-glucuronate
- Y150 (= Y72) binding UDP-alpha-D-glucuronate
- L159 (≠ F82) binding NAD(+)
- S161 (≠ A84) binding NAD(+)
- K177 (≠ D100) binding UDP-alpha-D-glucuronate
- T178 (≠ A101) binding NAD(+)
- N185 (= N108) binding UDP-alpha-D-glucuronate
- G188 (≠ E111) binding UDP-alpha-D-glucuronate
- K191 (≠ R114) binding UDP-alpha-D-glucuronate
- R192 (≠ E115) binding UDP-alpha-D-glucuronate
- A200 (= A124) binding NAD(+)
- E204 (≠ S128) mutation to A: Reduced UDP-glucuronic acid decarboxylase activity.
- Y231 (= Y151) active site, Proton acceptor; binding NAD(+); mutation to F: Abolished UDP-glucuronic acid decarboxylase activity.
- K235 (= K155) binding NAD(+)
- R236 (≠ K156) mutation to H: Strongly reduced UDP-glucuronic acid decarboxylase activity, caused by a local unfolding of the active site that allows for a rotation of the dimer interface, leading to the formation of a homohexamer.
- Y245 (= Y165) binding UDP-alpha-D-glucuronate
- Q248 (≠ L168) binding UDP-alpha-D-glucuronate
- E249 (≠ F169) binding UDP-alpha-D-glucuronate
- T261 (≠ V181) binding NAD(+)
- H267 (vs. gap) binding NAD(+)
- R272 (= R187) binding NAD(+)
- R361 (≠ T300) mutation to Q: Strongly reduced UDP-glucuronic acid decarboxylase activity.
Sites not aligning to the query:
- 1 modified: N-acetylmethionine
8wovB Crystal structure of arabidopsis thaliana udp-glucose 4-epimerase 2 (atuge2) complexed with udp, g233a mutant (see paper)
27% identity, 97% coverage: 3:329/337 of query aligns to 5:333/341 of 8wovB
- binding nicotinamide-adenine-dinucleotide: G9 (= G7), G12 (= G10), Y13 (≠ F11), I14 (= I12), D33 (= D31), N34 (≠ G32), D36 (≠ T34), N37 (≠ H35), S38 (≠ Y36), D63 (≠ L61), L64 (= L62), F85 (= F82), A86 (= A83), G87 (≠ A84), K89 (≠ A86), N104 (≠ A101), S127 (≠ A124), S129 (≠ T126), Y153 (= Y151), K157 (= K155), Y181 (≠ F178), P184 (≠ V181)
- binding uridine-5'-diphosphate: N183 (≠ T180), N203 (≠ A191), L204 (= L192), L219 (≠ I205), T220 (≠ D206), F222 (= F208), R235 (≠ G221), Y237 (≠ L223), R297 (≠ Q293), D300 (= D296)
1i3lA Molecular basis for severe epimerase-deficiency galactosemia: x-ray structure of the human v94m-substituted udp-galactose 4-epimerase (see paper)
28% identity, 98% coverage: 2:332/337 of query aligns to 3:338/347 of 1i3lA
- active site: S131 (≠ T126), A132 (≠ S127), T133 (≠ S128), Y156 (= Y151), K160 (= K155)
- binding galactose-uridine-5'-diphosphate: N186 (≠ T180), N206 (≠ A191), L207 (= L192), N223 (≠ D206), V224 (= V207), F225 (= F208), R238 (= R215), Y240 (≠ F217), V276 (≠ L270), R299 (≠ Q293), D302 (= D296)
- binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G10), Y12 (≠ F11), I13 (= I12), D32 (= D31), N33 (≠ G32), H35 (≠ T34), N36 (≠ H35), D65 (≠ A59), I66 (= I60), F87 (= F82), A88 (= A83), G89 (≠ A84), K91 (≠ A86), S129 (≠ A124), S130 (= S125), S131 (≠ T126), Y156 (= Y151), K160 (= K155), Y184 (≠ F178), P187 (≠ V181)
1i3kA Molecular basis for severe epimerase-deficiency galactosemia: x-ray structure of the human v94m-substituted udp-galactose 4-epimerase (see paper)
28% identity, 98% coverage: 2:332/337 of query aligns to 3:338/347 of 1i3kA
- active site: S131 (≠ T126), A132 (≠ S127), T133 (≠ S128), Y156 (= Y151), K160 (= K155)
- binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G10), Y12 (≠ F11), I13 (= I12), D32 (= D31), N33 (≠ G32), H35 (≠ T34), N36 (≠ H35), D65 (≠ A59), I66 (= I60), F87 (= F82), A88 (= A83), G89 (≠ A84), K91 (≠ A86), S129 (≠ A124), S131 (≠ T126), Y156 (= Y151), K160 (= K155), Y184 (≠ F178), P187 (≠ V181)
- binding uridine-5'-diphosphate-glucose: F185 (= F179), N186 (≠ T180), N206 (≠ A191), L207 (= L192), N223 (≠ D206), V224 (= V207), F225 (= F208), R238 (= R215), Y240 (≠ F217), V276 (≠ L270), R299 (≠ Q293), D302 (= D296)
1ek6A Structure of human udp-galactose 4-epimerase complexed with nadh and udp-glucose (see paper)
28% identity, 98% coverage: 2:332/337 of query aligns to 4:339/346 of 1ek6A
- active site: S132 (≠ T126), A133 (≠ S127), T134 (≠ S128), Y157 (= Y151), K161 (= K155)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G9 (= G7), G12 (= G10), Y13 (≠ F11), I14 (= I12), D33 (= D31), N34 (≠ G32), H36 (≠ T34), N37 (≠ H35), D66 (≠ A59), I67 (= I60), F88 (= F82), A89 (= A83), G90 (≠ A84), K92 (≠ A86), S130 (≠ A124), S131 (= S125), S132 (≠ T126), Y157 (= Y151), K161 (= K155), Y185 (≠ F178), P188 (≠ V181)
- binding uridine-5'-diphosphate-glucose: S132 (≠ T126), Y157 (= Y151), F186 (= F179), N187 (≠ T180), N207 (≠ A191), L208 (= L192), N224 (≠ D206), V225 (= V207), F226 (= F208), R239 (= R215), Y241 (≠ F217), V277 (≠ L270), R300 (≠ Q293), D303 (= D296)
Q14376 UDP-glucose 4-epimerase; Galactowaldenase; UDP-N-acetylgalactosamine 4-epimerase; UDP-GalNAc 4-epimerase; UDP-N-acetylglucosamine 4-epimerase; UDP-GlcNAc 4-epimerase; UDP-galactose 4-epimerase; EC 5.1.3.2; EC 5.1.3.7 from Homo sapiens (Human) (see 13 papers)
28% identity, 98% coverage: 2:332/337 of query aligns to 4:339/348 of Q14376
- GYI 12:14 (≠ GFI 10:12) binding NAD(+)
- DNFHN 33:37 (≠ DGFTH 31:35) binding NAD(+)
- N34 (≠ G32) to S: in GALAC3; peripheral; nearly normal activity towards UDP-galactose; dbSNP:rs121908046
- DI 66:67 (≠ AI 59:60) binding NAD(+)
- F88 (= F82) binding NAD(+)
- G90 (≠ A84) to E: in GALAC3; 800-fold decrease in UDP-galactose epimerization activity; dbSNP:rs28940882
- K92 (≠ A86) binding NAD(+)
- V94 (= V88) to M: in GALAC3; generalized; 30-fold decrease in UDP-galactose epimerization activity; 2-fold decrease in affinity for UDP-galactose; 24% of normal activity with respect to UDP-N-acetylgalactosamine; dbSNP:rs121908047
- D103 (≠ A97) to G: in GALAC3; 7-fold decrease in UDP-galactose epimerization activity; very mild decrease in activity towards UDP-N-acetylgalactosamine; dbSNP:rs28940883
- S132 (≠ T126) mutation to A: Loss of activity.
- SAT 132:134 (≠ TSS 126:128) binding substrate
- Y157 (= Y151) active site, Proton acceptor; mutation to F: Loss of activity.
- K161 (= K155) binding NAD(+)
- A180 (≠ V173) to V: in dbSNP:rs3204468
- L183 (= L176) to P: in GALAC3; peripheral; 3-fold decrease in UDP-galactose epimerization activity; dbSNP:rs121908045
- Y185 (≠ F178) binding NAD(+)
- YFN 185:187 (≠ FFT 178:180) binding substrate
- N-NL 206:208 (≠ DMAL 189:192) binding substrate
- NVF 224:226 (≠ DVF 206:208) binding substrate
- R239 (= R215) binding substrate
- K257 (= K233) to R: in GALAC3; 7-fold decrease in UDP-galactose epimerization activity; does not affect affinity for UDP-galactose; dbSNP:rs28940884
- REGD 300:303 (≠ QKGD 293:296) binding substrate
- C307 (≠ T300) mutation to Y: No effect on activity towards UDP-galactose. Loss of activity towards UDP-N-acetylgalactosamine.
- L313 (= L306) to M: in GALAC3; 6-fold decrease in UDP-galactose epimerization activity; very mild decrease in activity towards UDP-N-acetylgalactosamine; dbSNP:rs3180383
- G319 (= G312) to E: in GALAC3; nearly normal activity towards UDP-galactose; mild impairment under conditions of substrate limitation; dbSNP:rs28940885
- R335 (≠ D328) to H: in GALAC3; 2-fold decrease in UDP-galactose epimerization activity; dbSNP:rs368637540
6bwlA X-ray structure of pal from bacillus thuringiensis (see paper)
28% identity, 99% coverage: 1:332/337 of query aligns to 1:311/313 of 6bwlA
- active site: T122 (= T126), C123 (≠ S127), M124 (≠ S128), Y147 (= Y151), K151 (= K155)
- binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (= I12), D31 (= D31), N32 (≠ G32), L33 (≠ F33), N35 (≠ H35), S36 (≠ Y36), D57 (≠ I48), I58 (≠ L49), L79 (≠ F82), A80 (= A83), A81 (= A84), I83 (≠ A86), M120 (= M123), K151 (= K155), N176 (≠ T180), T177 (≠ V181)
- binding uridine-5'-diphosphate: N176 (≠ T180), G189 (≠ M190), V190 (≠ A191), N205 (≠ D206), I206 (≠ V207), Y207 (≠ F208), Q212 (≠ M213), R214 (= R215), I250 (≠ L270), E275 (= E291)
Query Sequence
>WP_012590207.1 NCBI__GCF_000021745.1:WP_012590207.1
MRILVTGSAGFIGFHMAARLLADGHEVVGVDGFTHYYDPELKRRRNAILSQNPYFTSHAI
LLEDASALKRVYDAGFDAVYHFAAQAGVRYSLENPRAYVDANLTGAFNLLELMREAPPKH
ALMASTSSVYGANTKIPFHETDRADHPLTFYAATKKANEEMAHSYAHLFKIPVTMLRFFT
VYGPWGRPDMALFKFVDAMVEGRPIDVFNHGKMKRDFTFVGDLVEAMALLIDKAPPAPDS
RTSPTPDYDSLSPVAPWRIVNIGTERPVGLMDFIEAIETATGLKAERNYLEMQKGDVPLT
FASTRLLFELTGYRPATTLAEGVKAFVDWRRSYLTQQ
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory