SitesBLAST
Comparing WP_012591939.1 NCBI__GCF_000021745.1:WP_012591939.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3ijpB Crystal structure of dihydrodipicolinate reductase from bartonella henselae at 2.0a resolution (see paper)
53% identity, 98% coverage: 4:269/272 of query aligns to 2:267/267 of 3ijpB
3ijpA Crystal structure of dihydrodipicolinate reductase from bartonella henselae at 2.0a resolution (see paper)
53% identity, 97% coverage: 4:268/272 of query aligns to 2:266/266 of 3ijpA
- active site: H155 (= H157), K159 (= K161)
- binding sodium ion: I21 (≠ V23), Q22 (= Q24), R24 (≠ T26), V27 (≠ A29)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G10), N10 (≠ A12), G11 (= G13), R12 (= R14), M13 (= M15), R35 (= R37), F75 (= F77), S76 (≠ T78), Q77 (≠ S79), A80 (≠ S82), G98 (= G100), T100 (= T102), G123 (= G125), N124 (= N126), M125 (= M127), F239 (= F241)
4ywjA Crystal structure of 4-hydroxy-tetrahydrodipicolinate reductase (htpa reductase) from pseudomonas aeruginosa
51% identity, 97% coverage: 5:268/272 of query aligns to 3:267/268 of 4ywjA
- active site: H156 (= H157), K160 (= K161)
- binding nicotinamide-adenine-dinucleotide: G11 (= G13), R12 (= R14), M13 (= M15), D35 (≠ A36), R36 (= R37), F76 (= F77), T77 (= T78), V81 (≠ S82), G99 (= G100), T101 (= T102), A124 (≠ G125), N125 (= N126), F126 (≠ M127), R237 (= R238), F240 (= F241)
5tejB Structure of 4-hydroxy-tetrahydrodipicolinate reductase from vibrio vulnificus with 2,5 furan dicarboxylic and nadh (see paper)
46% identity, 98% coverage: 4:270/272 of query aligns to 2:268/269 of 5tejB
- active site: H155 (= H157), K159 (= K161)
- binding 2,5 Furan Dicarboxylic Acid: T100 (= T102), H156 (= H158), K159 (= K161), S164 (= S166), G165 (= G167), T166 (= T168)
- binding nicotinamide-adenine-dinucleotide: G8 (= G10), G11 (= G13), R12 (= R14), M13 (= M15), E34 (≠ A36), R35 (= R37), F75 (= F77), T76 (= T78), S80 (= S82), G98 (= G100), T100 (= T102), P123 (≠ G125), N124 (= N126), Y125 (≠ M127), F239 (= F241)
5tejA Structure of 4-hydroxy-tetrahydrodipicolinate reductase from vibrio vulnificus with 2,5 furan dicarboxylic and nadh (see paper)
46% identity, 98% coverage: 4:270/272 of query aligns to 2:268/269 of 5tejA
- active site: H155 (= H157), K159 (= K161)
- binding nicotinamide-adenine-dinucleotide: G8 (= G10), G11 (= G13), R12 (= R14), M13 (= M15), E34 (≠ A36), R35 (= R37), F75 (= F77), T76 (= T78), S80 (= S82), G98 (= G100), T100 (= T102), P123 (≠ G125)
5temA Structure of 4-hydroxy-tetrahydrodipicolinate reductase from vibrio vulnificus with 2,6 pyridine dicarboxylic and nadh (see paper)
46% identity, 97% coverage: 4:268/272 of query aligns to 2:266/266 of 5temA
- active site: H155 (= H157), K159 (= K161)
- binding nicotinamide-adenine-dinucleotide: G8 (= G10), G11 (= G13), R12 (= R14), M13 (= M15), E34 (≠ A36), R35 (= R37), F75 (= F77), T76 (= T78), S80 (= S82), G98 (= G100), T100 (= T102), P123 (≠ G125), N124 (= N126), Y125 (≠ M127), F239 (= F241)
- binding pyridine-2,6-dicarboxylic acid: T100 (= T102), P123 (≠ G125), H156 (= H158), K159 (= K161), S164 (= S166), G165 (= G167), T166 (= T168)
1arzB Escherichia coli dihydrodipicolinate reductase in complex with nadh and 2,6 pyridine dicarboxylate (see paper)
46% identity, 99% coverage: 3:270/272 of query aligns to 1:268/269 of 1arzB
- active site: H155 (= H157), K159 (= K161)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G8 (= G10), G10 (≠ A12), G11 (= G13), R12 (= R14), M13 (= M15), E34 (≠ A36), F75 (= F77), T76 (= T78), R77 (≠ S79), G80 (≠ S82), H84 (≠ L86), G98 (= G100), T100 (= T102), A123 (≠ G125), N124 (= N126), F125 (≠ M127), F239 (= F241)
- binding pyridine-2,6-dicarboxylic acid: T100 (= T102), H156 (= H158), K159 (= K161), S164 (= S166), G165 (= G167), T166 (= T168), F239 (= F241)
1drvA Escherichia coli dhpr/acnadh complex (see paper)
46% identity, 99% coverage: 3:270/272 of query aligns to 2:269/270 of 1drvA
- active site: H156 (= H157), K160 (= K161)
- binding 3-acetylpyridine adenine dinucleotide: G9 (= G10), G12 (= G13), R13 (= R14), M14 (= M15), E35 (≠ A36), F76 (= F77), T77 (= T78), R78 (≠ S79), G81 (≠ S82), G99 (= G100), A124 (≠ G125), F126 (≠ M127), R237 (= R238)
1druA Escherichia coli dhpr/nadh complex (see paper)
46% identity, 99% coverage: 3:270/272 of query aligns to 2:269/270 of 1druA
- active site: H156 (= H157), K160 (= K161)
- binding nicotinamide-adenine-dinucleotide: G9 (= G10), G12 (= G13), R13 (= R14), M14 (= M15), E35 (≠ A36), R36 (= R37), F76 (= F77), T77 (= T78), R78 (≠ S79), G81 (≠ S82), G99 (= G100), T100 (= T101), T101 (= T102), A124 (≠ G125), N125 (= N126), F126 (≠ M127), F240 (= F241)
1arzA Escherichia coli dihydrodipicolinate reductase in complex with nadh and 2,6 pyridine dicarboxylate (see paper)
46% identity, 99% coverage: 3:270/272 of query aligns to 2:269/270 of 1arzA
1drwA Escherichia coli dhpr/nhdh complex (see paper)
46% identity, 99% coverage: 3:270/272 of query aligns to 4:271/272 of 1drwA
- active site: H158 (= H157), K162 (= K161)
- binding nicotinamide purin-6-ol-dinucleotide: G11 (= G10), G14 (= G13), R15 (= R14), M16 (= M15), E37 (≠ A36), R38 (= R37), F78 (= F77), T79 (= T78), R80 (≠ S79), G101 (= G100), T102 (= T101), T103 (= T102), A126 (≠ G125), N127 (= N126), F128 (≠ M127), F242 (= F241)
1dihA Three-dimensional structure of e. Coli dihydrodipicolinate reductase (see paper)
46% identity, 99% coverage: 3:270/272 of query aligns to 4:271/272 of 1dihA
- active site: H158 (= H157), K162 (= K161)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (= G10), G14 (= G13), R15 (= R14), M16 (= M15), R38 (= R37), F78 (= F77), T79 (= T78), R80 (≠ S79), G83 (≠ S82), G101 (= G100), T103 (= T102), N127 (= N126), F128 (≠ M127), R239 (= R238), F242 (= F241)
P04036 4-hydroxy-tetrahydrodipicolinate reductase; HTPA reductase; EC 1.17.1.8 from Escherichia coli (strain K12) (see 3 papers)
46% identity, 99% coverage: 3:270/272 of query aligns to 5:272/273 of P04036
- G12 (= G10) binding
- GRM 15:17 (= GRM 13:15) binding
- RM 16:17 (= RM 14:15) binding
- E38 (≠ A36) binding
- R39 (= R37) binding
- TR 80:81 (≠ TS 78:79) binding
- GTT 102:104 (= GTT 100:102) binding ; binding
- AANF 126:129 (≠ SGNM 124:127) binding
- F129 (≠ M127) binding
- H159 (= H157) mutation H->A,Q: 135 to 200-fold reduction in catalytic activity.
- K163 (= K161) binding ; mutation K->A,C,Q: 625 to 830-fold reduction in catalytic activity.
- R240 (= R238) binding
- F243 (= F241) binding
Q9X1K8 4-hydroxy-tetrahydrodipicolinate reductase; HTPA reductase; EC 1.17.1.8 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
36% identity, 78% coverage: 55:266/272 of query aligns to 26:212/216 of Q9X1K8
Sites not aligning to the query:
1vm6B Crystal structure of dihydrodipicolinate reductase (tm1520) from thermotoga maritima at 2.27 a resolution
36% identity, 78% coverage: 55:266/272 of query aligns to 31:217/218 of 1vm6B
- active site: H132 (= H157), K136 (= K161)
- binding nicotinamide-adenine-dinucleotide: D37 (≠ S61), V38 (≠ T62), F53 (= F77), S54 (≠ T78), S55 (= S79), E57 (≠ A81), A58 (≠ S82), G76 (= G100), T78 (= T102), Y101 (≠ G125), N102 (= N126), F103 (≠ M127), F192 (= F241)
Sites not aligning to the query:
5z2fA NADPH/pda bound dihydrodipicolinate reductase from paenisporosarcina sp. Tg-14 (see paper)
27% identity, 97% coverage: 4:266/272 of query aligns to 3:265/265 of 5z2fA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: R11 (≠ A12), G12 (= G13), K13 (≠ R14), M14 (= M15), D35 (≠ A36), H36 (≠ R37), K37 (≠ A38), L76 (≠ F77), T77 (= T78), G99 (= G100), T100 (= T101), T101 (= T102), P126 (≠ G125), N127 (= N126), F128 (≠ M127)
- binding pyridine-2,6-dicarboxylic acid: P126 (≠ G125), H155 (= H157), H156 (= H158), K159 (= K161), S164 (= S166), G165 (= G167), T166 (= T168), A215 (≠ G216)
5z2eA Dipicolinate bound dihydrodipicolinate reductase from paenisporosarcina sp. Tg-14 (see paper)
27% identity, 97% coverage: 4:266/272 of query aligns to 3:265/265 of 5z2eA
5wolA Crystal structure of dihydrodipicolinate reductase dapb from coxiella burnetii
31% identity, 90% coverage: 4:249/272 of query aligns to 2:213/230 of 5wolA
- active site: H133 (= H157), K137 (= K161)
- binding pyridine-2-carboxylic acid: P104 (≠ G125), T144 (= T168), K147 (≠ L171)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: N7 (≠ A9), G11 (= G13), K12 (≠ R14), M13 (= M15), G34 (≠ A36), R35 (= R37), F54 (= F77), T55 (= T78), T56 (≠ S79), S59 (= S82), G77 (= G100), T78 (= T101), T79 (= T102), P104 (≠ G125), N105 (= N126), F106 (≠ M127)
1p9lA Structure of m. Tuberculosis dihydrodipicolinate reductase in complex with nadh and 2,6 pdc (see paper)
30% identity, 90% coverage: 4:248/272 of query aligns to 1:224/245 of 1p9lA
- active site: H132 (= H157), K136 (= K161)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G7 (= G10), G10 (= G13), K11 (≠ R14), V12 (≠ M15), D33 (= D46), A34 (= A47), F52 (= F77), T53 (= T78), V57 (≠ S82), G75 (= G100), T77 (= T102), P103 (≠ G125), N104 (= N126), F105 (≠ M127), F217 (= F241)
- binding pyridine-2,6-dicarboxylic acid: H133 (= H158), K136 (= K161), S141 (= S166), G142 (= G167), T143 (= T168), A192 (≠ G216)
1c3vA Dihydrodipicolinate reductase from mycobacterium tuberculosis complexed with NADPH and pdc (see paper)
30% identity, 90% coverage: 4:248/272 of query aligns to 1:224/245 of 1c3vA
- active site: H132 (= H157), K136 (= K161)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: K9 (≠ A12), G10 (= G13), K11 (≠ R14), V12 (≠ M15), D33 (= D46), A34 (= A47), F52 (= F77), T53 (= T78), V57 (≠ S82), G75 (= G100), T77 (= T102), P103 (≠ G125), N104 (= N126), F217 (= F241)
- binding pyridine-2,6-dicarboxylic acid: T77 (= T102), N104 (= N126), K136 (= K161), S141 (= S166), G142 (= G167), T143 (= T168), A192 (≠ G216)
Query Sequence
>WP_012591939.1 NCBI__GCF_000021745.1:WP_012591939.1
MGDMRLVVAGAAGRMGRVLVQIVQQTPGAMVSGALARADSPAIGQDAGVLAGCGEIGVKI
STDPLRAVADCDGVLDFTSPASTMVLATLAAQARVVHVIGTTGLSPEHLARLDAAARHAV
IVQSGNMSLGVNLLAALVEKAARTLGPEFDIEIAEMHHRAKVDAPSGTALLLGEAAAKGR
NIDLPSHSLRARDGHTGARPEGAIGFASLRGGGVVGEHKVYFAGAGERLELAHVAEDRSI
FARGAVKAALWGRGRKSGLYSMADVLGLGDKI
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory