SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012592214.1 NCBI__GCF_000021745.1:WP_012592214.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

A0R5R7 Putative aminotransferase MSMEG_6286/MSMEI_6121 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
53% identity, 94% coverage: 7:413/431 of query aligns to 6:415/428 of A0R5R7

query
sites
A0R5R7

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K339 (≠ G337) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

O69689 Aspartate aminotransferase; AspAT; EC 2.6.1.1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
51% identity, 99% coverage: 4:431/431 of query aligns to 3:431/435 of O69689

query
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O69689

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Y69 (= Y71) binding pyridoxal 5'-phosphate
SL 100:101 (= SL 102:103) binding pyridoxal 5'-phosphate
YDR 139:141 (= YDR 140:142) binding substrate
N189 (= N189) binding pyridoxal 5'-phosphate
Y221 (= Y221) binding pyridoxal 5'-phosphate
STS 254:256 (= STS 254:256) binding pyridoxal 5'-phosphate
R392 (= R392) binding substrate

6u7aD Rv3722c in complex with kynurenine (see paper)
51% identity, 99% coverage: 4:430/431 of query aligns to 2:429/429 of 6u7aD

query
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6u7aD

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S

binding 4-hydroxyquinoline-2-carboxylic acid: G36 (= G38), Y138 (= Y140), R140 (= R142), K256 (= K257), F348 (= F349)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: S98 (= S101), S99 (= S102), L100 (= L103), Y138 (= Y140), D217 (= D218), A219 (= A220), Y220 (= Y221), S253 (= S254), S255 (= S256), K256 (= K257)

5c6uA Rv3722c aminotransferase from mycobacterium tuberculosis
52% identity, 98% coverage: 4:426/431 of query aligns to 2:425/427 of 5c6uA

query
sites
5c6uA

I

F

D

D

Q

S

L

L

S

S

K

P

A

Q

E

E

L

L

A

A

L

L

R

H

A

A

S

R

V

H

Q

Q

A

Q

N

D

Y

Y

D

A

A

A

F

L

R

Q

A

G

Q

M

G

K

L

L

K

A

L

L

D

D

M

L

T

T

R

R

G

G

K

K

P

P

A

S

P

A

D

E

Q

Q

L

L

D

D

L

L

A

S

N

N

A

Q

M

L

L

L

S

S

L

L

M

P

G

G

N

D

A

-

D

D

Y

Y

R

R

S

D

E

P

A

E

G

G

E

T

D

D

A

T

R

R

N

N

Y

Y

G

G

N

G

L

Q

Q

H

G

G

L

L

A

P

E

G

A

L

R

R

A

A

L

I

I

F

A

A

P

E

I

L

L

L

G

G

A

I

P

A

P

V

E

P

Q

N

I

L

V

I

V

A

G

G

D

N

N

N

S

S

S
|
S

L
|
L

A

E

L

L

M

M

H

H

D

D

S

I

L

V

V

A

Y

F

A

S

L

M

T

L

K

Y

G

G

V

G

P

V

G

D

S

S

A

P

R

R

P

P

W

W

A

I

K

Q

E

E

E

Q

S

D

-

G

V

I

A

K

F

F

I

L

C

C

P

P

A

V

P

P

G

G

Y
|
Y

D

D

R

R

H

H

F

F

A

A

I

I

C

T

E

E

E

T

Y

M

G

G

V

I

R

E

M

M

L

I

P

P

V

I

R

P

M

M

T

L

G

Q

E

D

G

G

P

P

D

D

M

V

E

D

E

L

V

I

E

E

R

E

L

L

A

V

A

A

-

V

D

D

P

P

A

A

V

I

K

K

G

G

I

M

W

W

A

T

V

V

P

P

K

V

Y

F

S

G

N

N

P

P

T

S

G

G

D

V

I

T

Y

Y

S

S

D

W

A

E

T

T

V

V

A

R

R

R

L

L

A

V

A

Q

M

M

R

R

T

T

G

A

A

A

R

P

D

D

F

F

R

R

I

L

F

F

W

W

D
|
D

D

N

A
|
A

Y
|
Y

C

A

V

V

H

H

H

T

L

L

T

T

A

L

Q

D

R

F

N

P

A

R

V

Q

A

V

N

D

I

V

L

L

E

G

E

L

C

A

A

A

R

K

A

A

G

G

N

N

Q

P

D

N

R

R

A

P

F

Y

I

V

F

F

A

A

S
|
S

T

T

S
|
S

K
|
K

I

I

T

T

L

F

A

A

G

G

A

G

G

G

L

V

A

S

F

F

A

G

S

G

S

S

P

L

A

G

N

N

V

I

K

A

W

W

Y

Y

L

L

S

Q

S

Y

A

A

S

G

K

K

R

K

S

S

I

I

G

G

P

P

D

D

K

K

L

V

N

N

Q

Q

L

L

R

R

H

H

V

L

R

R

F

F

L

F

R

G

D

D

A

A

E

D

G

G

I

V

N

R

A

L

H

H

M

M

D

L

A

R

H

H

R

Q

A

Q

L

I

I

L

A

A

P

P

K

K

F

F

A

A

A

L

V

V

I

A

E

E

A

V

L

L

D

D

R

Q

H

R

L

L

A

S

G

E

T

S

G

K

A

I

A

A

N

S

W

W

S

T

V

E

P

P

Q

K

G

G

Y

Y

F

F

I

I

S

S

V

L

E

D

A

V

S

L

A

P

G

G

T

T

A

A

R

R

V

T

V

V

E

A

L

L

A

A

K

K

A

D

A

V

G

G

V

I

A

A

L

V

T

T

P

E

A

A

G

G

A

A

T

S

W

F

P

P

Y

Y

G

R

K

K

D

D

P

P

D

D

S

K

N

N

L

I

R

R

L

I

A

A

P

P

T

S

F

F

P

P

T

S

L

V

A

P

E

D

V

L

R

R

A

N

A

A

S

V

E

D

G

G

I

L

A

A

L

T

C

C

I

A

L

L

A

A

L

T

E

E

A

T

R

L

T

L

A

N

Q

Q

A

G

binding pyridoxal-5'-phosphate: S99 (= S102), L100 (= L103), Y138 (= Y140), D217 (= D218), A219 (= A220), Y220 (= Y221), S253 (= S254), S255 (= S256), K256 (= K257)

6u78B Rv3722c in complex with glutamic acid (see paper)
52% identity, 97% coverage: 4:421/431 of query aligns to 2:420/421 of 6u78B

query
sites
6u78B

I

F

D

D

Q

S

L

L

S

S

K

P

A

Q

E

E

L

L

A

A

L

L

R

H

A

A

S

R

V

H

Q

Q

A

Q

N

D

Y

Y

D

A

A

A

F

L

R

Q

A

G

Q

M

G

K

L

L

K

A

L

L

D

D

M

L

T

T

R

R

G
|
G

K

K

P

P

A

S

P

A

D

E

Q

Q

L

L

D

D

L

L

A

S

N

N

A

Q

M

L

L

L

S

S

L

L

M

P

G

G

N

D

A

-

D

D

Y

Y

R

R

S

D

E

P

A

E

G

G

E

T

D

D

A

T

R

R

N

N

Y

Y

G

G

N

G

L

Q

Q

H

G

G

L

L

A

P

E

G

A

L

R

R

A

A

L

I

I

F

A

A

P

E

I

L

L

L

G

G

A

I

P

A

P

V

E

P

Q

N

I

L

V

I

V

A

G

G

D

N

N

N

S
|
S

L
|
L

A

E

L

L

M

M

H

H

D

D

S

I

L

V

V

A

Y

F

A

S

L

M

T

L

K

Y

G

G

V

G

P

V

G

D

S

S

A

P

R

R

P

P

W

W

A

I

K

Q

E

E

E

Q

S

D

-

G

V

I

A

K

F

F

I

L

C

C

P

P

A

V

P

P

G

G

Y
|
Y

D

D

R

R

H

H

F

F

A

A

I

I

C

T

E

E

E

T

Y

M

G

G

V

I

R

E

M

M

L

I

P

P

V

I

R

P

M

M

T

L

G

Q

E

D

G

G

P

P

D

D

M

V

E

D

E

L

V

I

E

E

R

E

L

L

A

V

A

A

-

V

D

D

P

P

A

A

V

I

K

K

G

G

I

M

W

W

A

T

V

V

P

P

K

V

Y

F

S

G

N

N

P

P

T

S

G

G

D

V

I

T

Y

Y

S

S

D

W

A

E

T

T

V

V

A

R

R

R

L

L

A

V

A

Q

M

M

R

R

T

T

G

A

A

A

R

P

D

D

F

F

R

R

I

L

F

F

W

W

D
|
D

D

N

A
|
A

Y
|
Y

C

A

V

V

H

H

H

T

L

L

T

T

A

L

Q

D

R

F

N

P

A

R

V

Q

A

V

N

D

I

V

L

L

E

G

E

L

C

A

A

A

R

K

A

A

G

G

N

N

Q

P

D

N

R

R

A

P

F

Y

I

V

F

F

A

A

S
|
S

T

T

S
|
S

K

K

I

I

T

T

L

F

A

A

G

G

A

G

G

G

L

V

A

S

F

F

A

G

S

G

S

S

P

L

A

G

N

N

V

I

K

A

W

W

Y

Y

L

L

S

Q

S

Y

A

A

S

G

K

K

R

K

S

S

I

I

G

G

P

P

D

D

K

K

L

V

N

N

Q

Q

L

L

R

R

H

H

V

L

R

R

F

F

L

F

R

G

D

D

A

A

E

D

G

G

I

V

N

R

A

L

H

H

M

M

D

L

A

R

H

H

R

Q

A

Q

L

I

I

L

A

A

P

P

K

K

F

F

A

A

A

L

V

V

I

A

E

E

A

V

L

L

D

D

R

Q

H

R

L

L

A

S

G

E

T

S

G

K

A

I

A

A

N

S

W

W

S

T

V

E

P

P

Q

K

G

G

Y

Y

F

F

I

I

S

S

V

L

E

D

A

V

S

L

A

P

G

G

T

T

A

A

R

R

V

T

V

V

E

A

L

L

A

A

K

K

A

D

A

V

G

G

V

I

A

A

L

V

T

T

P

E

A

A

G

G

A

A

T

S

W

F

P

P

Y

Y

G

R

K

K

D

D

P

P

D

D

S

K

N

N

L

I

R
|
R

L

I

A

A

P

P

T

S

F

F

P

P

T

S

L

V

A

P

E

D

V

L

R

R

A

N

A

A

S

V

E

D

G

G

I

L

A

A

L

T

C

C

I

A

L

L

A

A

L

T

E

E

A

T

binding glutamic acid: G36 (= G38), R391 (= R392)
binding pyridoxal-5'-phosphate: S98 (= S101), S99 (= S102), L100 (= L103), Y138 (= Y140), D217 (= D218), A219 (= A220), Y220 (= Y221), S253 (= S254), S255 (= S256)

6u7aA Rv3722c in complex with kynurenine (see paper)
52% identity, 97% coverage: 4:421/431 of query aligns to 2:420/423 of 6u7aA

query
sites
6u7aA

I

F

D

D

Q

S

L

L

S

S

K

P

A

Q

E

E

L

L

A

A

L

L

R

H

A

A

S

R

V

H

Q

Q

A

Q

N

D

Y

Y

D

A

A

A

F

L

R

Q

A

G

Q

M

G

K

L

L

K

A

L

L

D

D

M

L

T

T

R
|
R

G
|
G

K
|
K

P

P

A

S

P

A

D

E

Q

Q

L

L

D

D

L

L

A

S

N

N

A

Q

M

L

L

L

S

S

L

L

M

P

G

G

N

D

A

-

D

D

Y

Y

R
|
R

S

D

E

P

A

E

G

G

E

T

D
|
D

A

T

R

R

N

N

Y

Y

G

G

N

G

L

Q

Q

H

G

G

L

L

A

P

E

G

A

L

R

R

A

A

L

I

I

F

A

A

P

E

I

L

L

L

G

G

A

I

P

A

P

V

E

P

Q

N

I

L

V

I

V

A

G

G

D

N

N

N

S
|
S

L
|
L

A

E

L

L

M

M

H

H

D

D

S

I

L

V

V

A

Y

F

A

S

L

M

T

L

K

Y

G

G

V

G

P

V

G

D

S

S

A

P

R

R

P

P

W

W

A

I

K

Q

E

E

E

Q

S

D

-

G

V

I

A

K

F

F

I

L

C

C

P

P

A

V

P

P

G

G

Y
|
Y

D

D

R
|
R

H

H

F

F

A

A

I

I

C

T

E

E

E

T

Y

M

G

G

V

I

R

E

M

M

L

I

P

P

V

I

R

P

M

M

T

L

G

Q

E

D

G

G

P

P

D

D

M

V

E

D

E

L

V

I

E

E

R

E

L

L

A

V

A

A

-

V

D

D

P

P

A

A

V

I

K

K

G

G

I

M

W

W

A

T

V

V

P

P

K

V

Y

F

S

G

N
|
N

P

P

T

S

G

G

D

V

I

T

Y

Y

S

S

D

W

A

E

T

T

V

V

A

R

R

R

L

L

A

V

A

Q

M

M

R

R

T

T

G

A

A

A

R

P

D

D

F

F

R

R

I

L

F

F

W

W

D
|
D

D

N

A
|
A

Y
|
Y

C

A

V

V

H

H

H

T

L

L

T

T

A

L

Q

D

R

F

N

P

A

R

V

Q

A

V

N

D

I

V

L

L

E

G

E

L

C

A

A

A

R

K

A

A

G

G

N

N

Q

P

D

N

R

R

A

P

F

Y

I

V

F

F

A

A

S
|
S

T

T

S
|
S

K
|
K

I

I

T

T

L

F

A

A

G

G

A

G

G

G

L

V

A

S

F

F

A

G

S

G

S

S

P

L

A

G

N

N

V

I

K

A

W

W

Y

Y

L

L

S

Q

S

Y

A

A

S

G

K

K

R

K

S

S

I

I

G

G

P

P

D

D

K

K

L

V

N

N

Q

Q

L

L

R

R

H

H

V

L

R

R

F

F

L

F

R

G

D

D

A

A

E

D

G

G

I

V

N

R

A

L

H

H

M

M

D

L

A

R

H

H

R

Q

A

Q

L

I

I

L

A

A

P

P

K

K

F

F

A

A

A

L

V

V

I

A

E

E

A

V

L

L

D

D

R

Q

H

R

L

L

A

S

G

E

T

S

G

K

A

I

A

A

N

S

W

W

S

T

V

E

P

P

Q

K

G

G

Y

Y

F
|
F

I

I

S

S

V

L

E

D

A

V

S

L

A

P

G

G

T

T

A

A

R

R

V

T

V

V

E

A

L

L

A

A

K

K

A

D

A

V

G

G

V

I

A

A

L

V

T

T

P

E

A

A

G

G

A

A

T

S

W

F

P

P

Y

Y

G

R

K

K

D

D

P

P

D

D

S

K

N

N

L

I

R
|
R

L

I

A

A

P

P

T

S

F

F

P

P

T

S

L

V

A

P

E

D

V

L

R

R

A

N

A

A

S

V

E

D

G

G

I

L

A

A

L

T

C

C

I

A

L

L

A

A

L

T

E

E

A

T

binding phosphate ion: R58 (= R61), D64 (= D67)
binding (2S)-4-(2-aminophenyl)-2-[(E)-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)amino]-4-oxobutanoic acid: R35 (= R37), G36 (= G38), K37 (= K39), S98 (= S101), S99 (= S102), L100 (= L103), Y138 (= Y140), R140 (= R142), N188 (= N189), D217 (= D218), A219 (= A220), Y220 (= Y221), S253 (= S254), S255 (= S256), K256 (= K257), F348 (= F349), R391 (= R392)

Q8NTR2 Aspartate aminotransferase; AspAT; EC 2.6.1.1 from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / BCRC 11384 / CCUG 27702 / LMG 3730 / NBRC 12168 / NCIMB 10025 / NRRL B-2784 / 534) (see paper)
49% identity, 95% coverage: 12:420/431 of query aligns to 20:428/432 of Q8NTR2

query
sites
Q8NTR2

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R45 (= R37) mutation to A: Loss of activity.
RG 45:46 (= RG 37:38) binding substrate
K47 (= K39) mutation to A: 40% decrease in activity.
Y79 (= Y71) mutation to A: Loss of activity.
S109 (= S101) mutation to A: 30% decrease in activity.
SSL 109:111 (= SSL 101:103) binding pyridoxal 5'-phosphate
S110 (= S102) mutation to A: 30% decrease in activity.
Y148 (= Y140) mutation to A: Loss of activity.
YDR 148:150 (= YDR 140:142) binding substrate
R150 (= R142) mutation to A: 80% decrease in activity.
N197 (= N189) binding pyridoxal 5'-phosphate; mutation to A: Loss of activity.
D226 (= D218) mutation to A: Loss of activity.
Y229 (= Y221) binding pyridoxal 5'-phosphate; mutation to A: Loss of activity.
STSK 262:265 (= STSK 254:257) binding pyridoxal 5'-phosphate
S264 (= S256) mutation to A: 30% decrease in activity.
K265 (= K257) mutation to A: Loss of activity.
R400 (= R392) binding substrate; mutation to A: Loss of activity.

5hxxA Crystal structure of aspat from corynebacterium glutamicum
49% identity, 95% coverage: 12:420/431 of query aligns to 11:419/424 of 5hxxA

query
sites
5hxxA

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binding 2-oxoglutaric acid: G37 (= G38), Y139 (= Y140), R141 (= R142), K256 (= K257)
binding glutamic acid: Y70 (= Y71), I286 (= I287)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: S100 (= S101), S101 (= S102), L102 (= L103), Y139 (= Y140), V183 (= V184), D217 (= D218), A219 (= A220), Y220 (= Y221), S253 (= S254), S255 (= S256), K256 (= K257)

3pplA Crystal structure of an aspartate transaminase (ncgl0237, cgl0240) from corynebacterium glutamicum atcc 13032 kitasato at 1.25 a resolution
49% identity, 95% coverage: 12:420/431 of query aligns to 13:421/424 of 3pplA

query
sites
3pplA

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A

E

E

E

V

L

R

E

A

V

A

A

S

M

E

D

G

G

I

V

A

A

L

T

C

C

I

V

L

L

A

A

L

A

E

E

binding pyridoxal-5'-phosphate: S102 (= S101), S103 (= S102), L104 (= L103), Y141 (= Y140), D219 (= D218), A221 (= A220), Y222 (= Y221), S255 (= S254), S257 (= S256), K258 (= K257)

5iwqB Crystal structure of aspartate aminotransferase (aspat) from corynebacterium glutamicum atcc 13032 (see paper)
49% identity, 95% coverage: 12:420/431 of query aligns to 11:419/424 of 5iwqB

query
sites
5iwqB

L

I

A

G

A

L

L

F

R

H

A

E

S

D

V

I

Q

K

A

R

N

K

Y

F

D

D

A

E

F

L

R

K

A

S

Q

K

G

N

L

L

K

K

L

L

D

D

M

L

T

T

R
|
R

G
|
G

K

K

P

P

A

S

P

S

D

E

Q

Q

L

L

D

D

L

F

A

A

N

D

A

E

M

L

L

L

S

A

L

L

M

P

G

G

N

K

A

G

D

D

Y

F

R

K

S

A

E

A

A

D

G

G

E

T

D

D

A

V

R

R

N

N

Y

Y

G

G

N

G

L

L

Q

D

G

G

L

I

A

V

E

D

A

I

R

R

A

Q

L

I

I

W

A

A

P

D

I

L

L

L

G

G

A

V

P

P

P

V

E

E

Q

Q

I

V

V

L

V

A

G

G

D

D

N

A

S
|
S

L
|
L

A

N

L

I

M

M

H

F

D

D

S

V

L

I

V

S

Y

W

A

S

L

Y

T

I

K

F

G

G

V

N

P

N

G

D

S

S

A

V

R

Q

P

P

W

W

A

S

K

K

E

E

S

T

V

V

A

K

F

W

I

I

C

C

P

P

A

V

P

P

G

G

Y
|
Y

D

D

R
|
R

H

H

F

F

A

S

I

I

C

T

E

E

E

R

Y

F

G

G

V

F

R

E

M

M

L

I

P

S

V

V

R

P

M

M

T

N

G

E

E

D

G

G

P

P

D

D

M

M

E

D

E

A

V

V

E

E

R

E

L

L

A

V

A

K

D

N

P

P

A

Q

V

V

K

K

G

G

I

M

W

W

A

V

V

V

P

P

K

V

Y

F

S

S

N

N

P

P

T

T

G

G

D

F

I

T

Y

V

S

T

D

E

A

D

T

V

V

A

A

K

R

R

L

L

A

S

A

A

M

M

R

E

T

T

G

A

A

A

R

P

D

D

F

F

R

R

I

V

F

V

W

W

D
|
D

D

N

A
|
A

Y
|
Y

C

A

V

V

H

H

H

T

L

L

T

T

A

D

Q

E

R

F

N

P

A

E

V

V

A

I

N

D

I

I

L

V

E

G

E

L

C

G

A

E

R

A

A

A

G

G

N

N

Q

P

D

N

R

R

A

F

F

W

I

A

F

F

A

T

S
|
S

T

T

S
|
S

K
|
K

I

I

T

T

L

L

A

A

G

G

A

A

G

G

L

V

A

S

F

F

A

L

S

T

S

S

P

A

A

E

N

N

V

R

K

K

W

W

Y

Y

L

T

S

G

S

H

A

A

S

G

K

I

R

R

S

G

I

I

G

G

P

P

D

N

K

K

L

V

N

N

Q

Q

L

L

R

A

H

H

V

A

R

R

F

Y

L

F

R

G

D

D

A

A

E

E

G

G

I

V

N

R

A

A

H

V

M

M

D

R

A

K

H

H

R

A

A

A

L

S

I

L

A

A

P

P

K

K

F

F

A

N

A

K

V

V

I

L

E

E

A

I

L

L

D

D

R

S

H

R

L

L

A

A

G

E

T

Y

G

G

A

V

A

A

N

Q

W

W

S

T

V

V

P

P

Q

A

G

G

Y

Y

F

F

I

I

S

S

V

L

E

D

A

V

S

V

A

P

G

G

T

T

A

A

R

S

R

R

V

V

V

A

E

E

L

L

A

A

K

K

A

E

A

A

G

G

V

I

A

A

L

L

T

T

P

G

A

A

G

G

A

S

T

S

W

Y

P

P

Y

L

G

R

K

Q

D

D

P

P

D

E

D

N

S

K

N

N

L

L

R

R

L

L

A

A

P

P

T

S

F

L

P

P

T

P

L

V

A

E

E

E

V

L

R

E

A

V

A

A

S

M

E

D

G

G

I

V

A

A

L

T

C

C

I

V

L

L

A

A

L

A

E

E

binding citrate anion: R36 (= R37), G37 (= G38), Y139 (= Y140), R141 (= R142), K256 (= K257)
binding pyridoxal-5'-phosphate: S100 (= S101), S101 (= S102), L102 (= L103), Y139 (= Y140), D217 (= D218), A219 (= A220), Y220 (= Y221), S253 (= S254), S255 (= S256), K256 (= K257)

3cbfA Crystal structure of lysn, alpha-aminoadipate aminotransferase, from thermus thermophilus hb27 (see paper)
26% identity, 77% coverage: 63:394/431 of query aligns to 58:366/392 of 3cbfA

query
sites
3cbfA

E

E

A

K

G

G

E

E

D

V

A

A

R

L

N

Q

Y

Y

G

S

N

P

L

T

Q

E

G

G

L

Y

A

A

E

P

A

L

R

R

A

A

L

F

I

V

A

A

P

E

I

W

L

I

G

G

A

V

P

R

P

P

E

E

Q

E

I

V

V

L

V

I

G

T

D

T

N
x
G

S
|
S

S
x
Q

L

Q

A

A

L

L

M

D

H

L

D

V

S

G

L

K

V

V

Y

F

A

-

L

L

T

D

K

E

G

G

V

S

P

P

G

-

S

-

A

-

R

-

P

-

W

-

A

-

K

-

E

-

S

-

V

-

A

-

F

V

I

L

C

L

P

E

A

A

P

P

G

S

Y
|
Y

D

M

R

G

H

A

F

I

A

Q

I

A

C

F

E

R

E

L

Y

Q

G

G

V

P

R

R

M

F

L

L

P

T

V

V

R

P

M

A

T

G

G

E

E

E

G

G

P

P

D

D

M

L

E

D

E

A

V

L

E

E

R

E

L

V

A

L

A

K

D

R

P

E

A

R

V

P

K

R

G

F

I

L

W

Y

A

L

V

I

P

P

K

S

Y

F

S

Q

N
|
N

P

P

T

T

G

G

D

G

I

L

Y

T

S

P

D

L

A

P

T

A

V

R

A

K

R

R

L

L

A

L

A

Q

M

M

R

-

T

V

G

M

A

E

R

R

D

G

F

L

R

V

I

V

F

V

W

E

D
|
D

D

D

A

A

Y
|
Y

C

R

V

E

H

L

H

Y

L

F

T

G

A

E

Q

A

R

R

N

-

A

-

V

-

A

L

N

P

I

S

L

L

E

F

E

E

C

L

A

A

R

R

A

E

G

A

N

G

Q

Y

D

P

R

G

A

V

F

I

I

Y

F

L

A

G

S
|
S

T

F

S
|
S

K
|
K

I

V

T

L

L

S

A

P

G

G

A

L

G
x
R

L

V

A

A

F

F

F

A

A

V

S

A

S

H

P

P

A

E

N

A

V

L

K

Q

W

K

Y

L

L

V

S

Q

S

A

A

K

S

Q

K

G

R

A

S

D

I

L

G

H

P

T

D

P

K

M

L

L

N

N

Q

Q

L

M

R

L

H

V

V

H

R

E

F

L

L

L

R

K

D

-

A

-

E

E

G

G

I

F

N

S

A

E

H

R

M

L

D

E

A

R

H

V

R

R

A

R

L

V

I

Y

A

R

P

E

K

K

F

A

A

Q

A

A

V

M

I

L

E

H

A

A

L

L

D

D

R

R

H

E

L

V

A

P

G

K

T

E

G

-

A

-

A

V

N

R

W

Y

S

T

V

R

P

P

Q

K

G

G

Y

M

F

F

I

V

S

W

V

M

E

E

A

L

S

P

A

K

G

G

-

L

T

S

A

A

R

E

R

G

V

L

V

F

E

R

L

R

A

A

K

L

A

E

G

N

V

V

A

A

L

F

T

V

P

P

A

G

G

G

A

P

T

F

W

F

P

A

Y

N

G

G

K

G

D

-

P

-

D

G

D

E

S

N

N

T

L

L

R
|
R

L

L

A

S

binding (2S)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]hexanedioic acid: G95 (≠ N100), S96 (= S101), Q97 (≠ S102), Y121 (= Y140), N170 (= N189), D198 (= D218), Y201 (= Y221), S231 (= S254), S233 (= S256), K234 (= K257), R241 (≠ G264), R364 (= R392)

Sites not aligning to the query:

binding (2S)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]hexanedioic acid: 18, 19, 35, 36

2egyA Crystal structure of lysn, alpha-aminoadipate aminotransferase (substrate free form), from thermus thermophilus hb27
26% identity, 77% coverage: 63:394/431 of query aligns to 58:366/392 of 2egyA

query
sites
2egyA

E

E

A

K

G

G

E

E

D

V

A

A

R

L

N

Q

Y

Y

G

S

N

P

L

T

Q

E

G

G

L

Y

A

A

E

P

A

L

R

R

A

A

L

F

I

V

A

A

P

E

I

W

L

I

G

G

A

V

P

R

P

P

E

E

Q

E

I

V

V

L

V

I

G

T

D

T

N
x
G

S
|
S

S
x
Q

L

Q

A

A

L

L

M

D

H

L

D

V

S

G

L

K

V

V

Y

F

A

-

L

L

T

D

K

E

G

G

V

S

P

P

G

-

S

-

A

-

R

-

P

-

W

-

A

-

K

-

E

-

S

-

V

-

A

-

F

V

I

L

C

L

P

E

A

A

P

P

G

S

Y
|
Y

D

M

R

G

H

A

F

I

A

Q

I

A

C

F

E

R

E

L

Y

Q

G

G

V

P

R

R

M

F

L

L

P

T

V

V

R

P

M

A

T

G

G

E

E

E

G

G

P

P

D

D

M

L

E

D

E

A

V

L

E

E

R

E

L

V

A

L

A

K

D

R

P

E

A

R

V

P

K

R

G

F

I

L

W

Y

A

L

V

I

P

P

K

S

Y

F

S

Q

N
|
N

P

P

T

T

G

G

D

G

I

L

Y

T

S

P

D

L

A

P

T

A

V

R

A

K

R

R

L

L

A

L

A

Q

M

M

R

-

T

V

G

M

A

E

R

R

D

G

F

L

R

V

I

V

F

V

W

E

D
|
D

D

D

A
|
A

Y
|
Y

C

R

V

E

H

L

H

Y

L

F

T

G

A

E

Q

A

R

R

N

-

A

-

V

-

A

L

N

P

I

S

L

L

E

F

E

E

C

L

A

A

R

R

A

E

G

A

N

G

Q

Y

D

P

R

G

A

V

F

I

I

Y

F

L

A

G

S
|
S

T

F

S
|
S

K
|
K

I

V

T

L

L

S

A

P

G

G

A

L

G
x
R

L

V

A

A

F

F

F

A

A

V

S

A

S

H

P

P

A

E

N

A

V

L

K

Q

W

K

Y

L

L

V

S

Q

S

A

A

K

S

Q

K

G

R

A

S

D

I

L

G

H

P

T

D

P

K

M

L

L

N

N

Q

Q

L

M

R

L

H

V

V

H

R

E

F

L

L

L

R

K

D

-

A

-

E

E

G

G

I

F

N

S

A

E

H

R

M

L

D

E

A

R

H

V

R

R

A

R

L

V

I

Y

A

R

P

E

K

K

F

A

A

Q

A

A

V

M

I

L

E

H

A

A

L

L

D

D

R

R

H

E

L

V

A

P

G

K

T

E

G

-

A

-

A

V

N

R

W

Y

S

T

V

R

P

P

Q

K

G

G

Y

M

F

F

I

V

S

W

V

M

E

E

A

L

S

P

A

K

G

G

-

L

T

S

A

A

R

E

R

G

V

L

V

F

E

R

L

R

A

A

K

L

A

E

G

N

V

V

A

A

L

F

T

V

P

P

A

G

G

G

A

P

T

F

W

F

P

A

Y

N

G

G

K

G

D

-

P

-

D

G

D

E

S

N

N

T

L

L

R

R

L

L

A

S

binding pyridoxal-5'-phosphate: G95 (≠ N100), S96 (= S101), Q97 (≠ S102), Y121 (= Y140), N170 (= N189), D198 (= D218), A200 (= A220), Y201 (= Y221), S231 (= S254), S233 (= S256), K234 (= K257), R241 (≠ G264)

2zyjA Crystal structure of lysn, alpha-aminoadipate aminotransferase (complexed with n-(5'-phosphopyridoxyl)-l-glutamate), from thermus thermophilus hb27 (see paper)
26% identity, 77% coverage: 63:394/431 of query aligns to 62:370/397 of 2zyjA

query
sites
2zyjA

E

E

A

K

G

G

E

E

D

V

A

A

R

L

N

Q

Y

Y

G

S

N

P

L

T

Q

E

G

G

L

Y

A

A

E

P

A

L

R

R

A

A

L

F

I

V

A

A

P

E

I

W

L

I

G

G

A

V

P

R

P

P

E

E

Q

E

I

V

V

L

V

I

G

T

D

T

N
x
G

S
|
S

S
x
Q

L

Q

A

A

L

L

M

D

H

L

D

V

S

G

L

K

V

V

Y

F

A

-

L

L

T

D

K

E

G

G

V

S

P

P

G

-

S

-

A

-

R

-

P

-

W

-

A

-

K

-

E

-

S

-

V

-

A

-

F

V

I

L

C

L

P

E

A

A

P

P

G

S

Y
|
Y

D

M

R

G

H

A

F

I

A

Q

I

A

C

F

E

R

E

L

Y

Q

G

G

V

P

R

R

M

F

L

L

P

T

V

V

R

P

M

A

T

G

G

E

E

E

G

G

P

P

D

D

M

L

E

D

E

A

V

L

E

E

R

E

L

V

A

L

A

K

D

R

P

E

A

R

V

P

K

R

G

F

I

L

W

Y

A

L

V

I

P

P

K

S

Y

F

S

Q

N
|
N

P

P

T

T

G

G

D

G

I

L

Y

T

S

P

D

L

A

P

T

A

V

R

A

K

R

R

L

L

A

L

A

Q

M

M

R

-

T

V

G

M

A

E

R

R

D

G

F

L

R

V

I

V

F

V

W

E

D
|
D

D

D

A

A

Y
|
Y

C

R

V

E

H

L

H

Y

L

F

T

G

A

E

Q

A

R

R

N

-

A

-

V

-

A

L

N

P

I

S

L

L

E

F

E

E

C

L

A

A

R

R

A

E

G

A

N

G

Q

Y

D

P

R

G

A

V

F

I

I

Y

F

L

A

G

S
|
S

T

F

S
|
S

K
|
K

I

V

T

L

L

S

A

P

G

G

A

L

G
x
R

L

V

A

A

F

F

F

A

A

V

S

A

S

H

P

P

A

E

N

A

V

L

K

Q

W

K

Y

L

L

V

S

Q

S

A

A

K

S

Q

K

G

R

A

S

D

I

L

G

H

P

T

D

P

K

M

L

L

N

N

Q

Q

L

M

R

L

H

V

V

H

R

E

F

L

L

L

R

K

D

-

A

-

E

E

G

G

I

F

N

S

A

E

H

R

M

L

D

E

A

R

H

V

R

R

A

R

L

V

I

Y

A

R

P

E

K

K

F

A

A

Q

A

A

V

M

I

L

E

H

A

A

L

L

D

D

R

R

H

E

L

V

A

P

G

K

T

E

G

-

A

-

A

V

N

R

W

Y

S

T

V

R

P

P

Q

K

G

G

Y

M

F

F

I

V

S

W

V

M

E

E

A

L

S

P

A

K

G

G

-

L

T

S

A

A

R

E

R

G

V

L

V

F

E

R

L

R

A

A

K

L

A

E

G

N

V

V

A

A

L

F

T

V

P

P

A

G

G

G

A

P

T

F

W

F

P

A

Y

N

G

G

K

G

D

-

P

-

D

G

D

E

S

N

N

T

L

L

R
|
R

L

L

A

S

binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: G99 (≠ N100), S100 (= S101), Q101 (≠ S102), Y125 (= Y140), N174 (= N189), D202 (= D218), Y205 (= Y221), S235 (= S254), S237 (= S256), K238 (= K257), R245 (≠ G264), R368 (= R392)

Sites not aligning to the query:

binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: 22, 23, 39, 40

Q72LL6 2-aminoadipate transaminase; 2-aminoadipate aminotransferase; Alpha-aminoadipate aminotransferase; AAA-AT; AadAT; EC 2.6.1.39 from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see 2 papers)
26% identity, 77% coverage: 63:394/431 of query aligns to 62:370/397 of Q72LL6

query
sites
Q72LL6

E

E

A

K

G

G

E

E

D

V

A

A

R

L

N

Q

Y
|
Y

G

S

N

P

L

T

Q

E

G

G

L

Y

A

A

E

P

A

L

R

R

A

A

L

F

I

V

A

A

P

E

I

W

L

I

G

G

A

V

P

R

P

P

E

E

Q

E

I

V

V

L

V

I

G

T

D

T

N

G

S

S

S

Q

L

Q

A

A

L

L

M

D

H

L

D

V

S

G

L

K

V

V

Y

F

A

-

L

L

T

D

K

E

G

G

V

S

P

P

G

-

S

-

A

-

R

-

P

-

W

-

A

-

K

-

E

-

S

-

V

-

A

-

F

V

I

L

C

L

P

E

A

A

P

P

G

S

Y

Y

D

M

R

G

H

A

F

I

A

Q

I

A

C

F

E

R

E

L

Y

Q

G

G

V

P

R

R

M

F

L

L

P

T

V

V

R

P

M

A

T

G

G

E

E

E

G

G

P

P

D

D

M

L

E

D

E

A

V

L

E

E

R

E

L

V

A

L

A

K

D

R

P

E

A

R

V

P

K

R

G

F

I

L

W

Y

A

L

V

I

P

P

K

S

Y

F

S

Q

N
|
N

P

P

T

T

G

G

D

G

I

L

Y

T

S

P

D

L

A

P

T

A

V

R

A

K

R

R

L

L

A

L

A

Q

M

M

R

-

T

V

G

M

A

E

R

R

D

G

F

L

R

V

I

V

F

V

W

E

D

D

A

A

Y

Y

C

R

V

E

H

L

H

Y

L

F

T

G

A

E

Q

A

R

R

N

-

A

-

V

-

A

L

N

P

I

S

L

L

E

F

E

E

C

L

A

A

R

R

A

E

G

A

N

G

Q

Y

D

P

R

G

A

V

F

I

I

Y

F

L

A

G

S

S

T

F

S

S

K

K

I

V

T

L

L

S

A

P

G

G

A

L

G
x
R

L

V

A

A

F

F

F

A

A

V

S

A

S

H

P

P

A

E

N

A

V

L

K

Q

W

K

Y

L

L

V

S

Q

S

A

A

K

S

Q

K

G

R

A

S

D

I

L

G

H

P

T

D

P

K

M

L

L

N

N

Q

Q

L

M

R

L

H

V

V

H

R

E

F

L

L

L

R

K

D

-

A

-

E

E

G

G

I

F

N

S

A

E

H

R

M

L

D

E

A

R

H

V

R

R

A

R

L

V

I

Y

A

R

P

E

K

K

F

A

A

Q

A

A

V

M

I

L

E

H

A

A

L

L

D

D

R

R

H

E

L

V

A

P

G

K

T

E

G

-

A

-

A

V

N

R

W

Y

S

T

V

R

P

P

Q

K

G

G

Y

M

F

F

I

V

S

W

V

M

E

E

A

L

S

P

A

K

G

G

-

L

T

S

A

A

R

E

R

G

V

L

V

F

E

R

L

R

A

A

K

L

A

E

G

N

V

V

A

A

L

F

T

V

P

P

A

G

G

G

A

P

T

F

W

F

P

A

Y

N

G

G

K

G

D

-

P

-

D

G

D

E

S

N

N

T

L

L

R
|
R

L

L

A

S

Y70 (= Y71) binding pyridoxal 5'-phosphate
N174 (= N189) binding pyridoxal 5'-phosphate; binding substrate
R245 (≠ G264) binding pyridoxal 5'-phosphate
R368 (= R392) binding substrate

Sites not aligning to the query:

20 S→E: Strongly decreases the affinity for AAA and Glu. A mild decrease of affinity is observed for 2-oxoglutarate. Increases the affinity for leucine and 2-oxoisocaproate.
23 R→A: Strongly decreases the affinity for AAA and Glu. A mild decrease of affinity is observed for 2-oxoglutarate which has the same chain length as Glu, but differs by the presence of a 2-oxo group which is not recognized by R-23. Increases the affinity for leucine and 2-oxoisocaproate due to the absence of gamma-carboxyl group.; R→Q: Strongly decreases the affinity for AAA and Glu. A mild decrease of affinity is observed for 2-oxoglutarate which has the same chain length as Glu, but differs by the presence of a 2-oxo group which is not recognized by R-23. Increases the affinity for leucine and 2-oxoisocaproate due to the absence of gamma-carboxyl group.
40 binding substrate

2z1yA Crystal structure of lysn, alpha-aminoadipate aminotransferase (complexed with n-(5'-phosphopyridoxyl)-l-leucine), from thermus thermophilus hb27
26% identity, 77% coverage: 63:394/431 of query aligns to 54:362/389 of 2z1yA

query
sites
2z1yA

E

E

A

K

G

G

E

E

D

V

A

A

R

L

N

Q

Y

Y

G

S

N

P

L

T

Q

E

G

G

L

Y

A

A

E

P

A

L

R

R

A

A

L

F

I

V

A

A

P

E

I

W

L

I

G

G

A

V

P

R

P

P

E

E

Q

E

I

V

V

L

V

I

G

T

D

T

N
x
G

S
|
S

S
x
Q

L

Q

A

A

L

L

M

D

H

L

D

V

S

G

L

K

V

V

Y

F

A

-

L

L

T

D

K

E

G

G

V

S

P

P

G

-

S

-

A

-

R

-

P

-

W

-

A

-

K

-

E

-

S

-

V

-

A

-

F

V

I

L

C

L

P

E

A

A

P

P

G

S

Y
|
Y

D

M

R

G

H

A

F

I

A

Q

I

A

C

F

E

R

E

L

Y

Q

G

G

V

P

R

R

M

F

L

L

P

T

V

V

R

P

M

A

T

G

G

E

E

E

G

G

P

P

D

D

M

L

E

D

E

A

V

L

E

E

R

E

L

V

A

L

A

K

D

R

P

E

A

R

V

P

K

R

G

F

I

L

W

Y

A

L

V

I

P

P

K

S

Y

F

S

Q

N
|
N

P

P

T

T

G

G

D

G

I

L

Y

T

S

P

D

L

A

P

T

A

V

R

A

K

R

R

L

L

A

L

A

Q

M

M

R

-

T

V

G

M

A

E

R

R

D

G

F

L

R

V

I

V

F

V

W

E

D
|
D

D

D

A

A

Y
|
Y

C

R

V

E

H

L

H

Y

L

F

T

G

A

E

Q

A

R

R

N

-

A

-

V

-

A

L

N

P

I

S

L

L

E

F

E

E

C

L

A

A

R

R

A

E

G

A

N

G

Q

Y

D

P

R

G

A

V

F

I

I

Y

F

L

A

G

S
|
S

T

F

S
|
S

K
|
K

I

V

T

L

L

S

A

P

G

G

A

L

G
x
R

L

V

A

A

F

F

F

A

A

V

S

A

S

H

P

P

A

E

N

A

V

L

K

Q

W

K

Y

L

L

V

S

Q

S

A

A

K

S

Q

K

G

R

A

S

D

I

L

G

H

P

T

D

P

K

M

L

L

N

N

Q

Q

L

M

R

L

H

V

V

H

R

E

F

L

L

L

R

K

D

-

A

-

E

E

G

G

I

F

N

S

A

E

H

R

M

L

D

E

A

R

H

V

R

R

A

R

L

V

I

Y

A

R

P

E

K

K

F

A

A

Q

A

A

V

M

I

L

E

H

A

A

L

L

D

D

R

R

H

E

L

V

A

P

G

K

T

E

G

-

A

-

A

V

N

R

W

Y

S

T

V

R

P

P

Q

K

G

G

Y

M

F

F

I

V

S

W

V

M

E

E

A

L

S

P

A

K

G

G

-

L

T

S

A

A

R

E

R

G

V

L

V

F

E

R

L

R

A

A

K

L

A

E

G

N

V

V

A

A

L

F

T

V

P

P

A

G

G

G

A

P

T

F

W

F

P

A

Y

N

G

G

K

G

D

-

P

-

D

G

D

E

S

N

N

T

L

L

R
|
R

L

L

A

S

binding leucine: Y117 (= Y140), R360 (= R392)
binding pyridoxal-5'-phosphate: G91 (≠ N100), S92 (= S101), Q93 (≠ S102), Y117 (= Y140), N166 (= N189), D194 (= D218), Y197 (= Y221), S227 (= S254), S229 (= S256), K230 (= K257), R237 (≠ G264)

Sites not aligning to the query:

binding leucine: 32

4ge4A Kynurenine aminotransferase ii inhibitors (see paper)
27% identity, 47% coverage: 179:380/431 of query aligns to 186:382/422 of 4ge4A

query
sites
4ge4A

K

K

G

F

I

L

W

Y

A

T

V

V

P

P

K

N

Y

G

S

N

N
|
N

P

P

T

T

G

G

D

N

I

-

Y

-

S

-

D

S

A

L

T

T

V

S

A

E

R

R

L

K

A

K

A

E

M

I

R

Y

T

E

G

L

A

A

R

R

-

K

-

Y

D

D

F

F

R

L

I

I

F

I

W

E

D
|
D

D

D

A
x
P

Y
|
Y

C

Y

V

F

H

L

H

Q

L

F

T

N

A

S

Q

G

R

R

N

V

A

P

V

T

A

F

N

L

I

S

L

M

E

D

E

V

C

D

A

G

R

R

A

V

G

I

N

R

Q

A

D

D

R

-

A

-

F

-

I

-

F

-

A

-

S
|
S

T

F

S
|
S

K
|
K

I

I

T

I

L

S

A

S

G

G

A

L

G
x
R

L

I

A

G

F

F

F

L

A

T

S

G

S

P

P

K

A

P

N

L

V

I

K

E

W

R

Y

V

L

I

S

L

S

H

A

I

S

Q

K

V

R

S

S

T

I

L

G

H

P

P

D

S

K

T

L

F

N

N

Q

Q

L

L

R

M

H

I

V

S

R

Q

F

L

L

L

R

H

D

E

-

W

-

G

A

E

E

E

G

G

I

F

N

M

A

A

H

H

M

V

D

D

A

R

H

V

R

I

A

D

L

F

I

Y

A

S

P

N

K

Q

F

K

A

D

A

A

V

I

I

L

E

A

A

A

L

A

D

D

R

K

H

W

L

L

A

-

G

-

T

T

G

G

A

L

A

A

N

E

W

W

S

H

V

V

P

P

Q

A

G

A

G

G

Y

M

F

F

-

L

-

W

I

I

S

K

V

V

E

K

A

G

S

I

A

N

G

D

T

V

A

K

R

E

R

L

V

I

V

E

E

E

L

K

A

A

K

V

A

K

A

M

G

G

V

V

A

L

L

M

T

L

P

P

A

G

G

N

A

A

T

F

W

Y

binding (5-hydroxy-4-{[(1-hydroxy-7-methoxy-2-oxo-1,2-dihydroquinolin-3-yl)amino]methyl}-6-methylpyridin-3-yl)methyl dihydrogen phosphate: N196 (= N189), D224 (= D218), P226 (≠ A220), Y227 (= Y221), S254 (= S254), S256 (= S256), K257 (= K257), R264 (≠ G264)

Sites not aligning to the query:

binding (5-hydroxy-4-{[(1-hydroxy-7-methoxy-2-oxo-1,2-dihydroquinolin-3-yl)amino]methyl}-6-methylpyridin-3-yl)methyl dihydrogen phosphate: 19, 34, 35, 36, 68, 110, 111, 112, 136, 393

4gebA Kynurenine aminotransferase ii inhibitors (see paper)
27% identity, 47% coverage: 179:380/431 of query aligns to 192:388/428 of 4gebA

query
sites
4gebA

K

K

G

F

I

L

W

Y

A

T

V

V

P

P

K

N

Y

G

S

N

N
|
N

P

P

T

T

G

G

D

N

I

-

Y

-

S

-

D

S

A

L

T

T

V

S

A

E

R

R

L

K

A

K

A

E

M

I

R

Y

T

E

G

L

A

A

R

R

-

K

-

Y

D

D

F

F

R

L

I

I

F

I

W

E

D
|
D

D

D

A
x
P

Y
|
Y

C

Y

V

F

H

L

H

Q

L

F

T

N

A

S

Q

G

R

R

N

V

A

P

V

T

A

F

N

L

I

S

L

M

E

D

E

V

C

D

A

G

R

R

A

V

G

I

N

R

Q

A

D

D

R

-

A

-

F

-

I

-

F

-

A

-

S
|
S

T

F

S
|
S

K
|
K

I

I

T

I

L

S

A

S

G

G

A

L

G
x
R

L

I

A

G

F

F

F

L

A

T

S

G

S

P

P

K

A

P

N

L

V

I

K

E

W

R

Y

V

L

I

S

L

S

H

A

I

S

Q

K

V

R

S

S

T

I

L

G

H

P

P

D

S

K

T

L

F

N

N

Q

Q

L

L

R

M

H

I

V

S

R

Q

F

L

L

L

R

H

D

E

-

W

-

G

A

E

E

E

G

G

I

F

N

M

A

A

H

H

M

V

D

D

A

R

H

V

R

I

A

D

L

F

I

Y

A

S

P

N

K

Q

F

K

A

D

A

A

V

I

I

L

E

A

A

A

L

A

D

D

R

K

H

W

L

L

A

-

G

-

T

T

G

G

A

L

A

A

N

E

W

W

S

H

V

V

P

P

Q

A

G

A

G

G

Y

M

F

F

-

L

-

W

I

I

S

K

V

V

E

K

A

G

S

I

A

N

G

D

T

V

A

K

R

E

R

L

V

I

V

E

E

E

L

K

A

A

K

V

A

K

A

M

G

G

V

V

A

L

L

M

T

L

P

P

A

G

G

N

A

A

T

F

W

Y

binding (5-hydroxy-4-{[(7-hydroxy-6-oxo-2-phenyl-6,7-dihydro-2H-pyrazolo[3,4-b]pyridin-5-yl)amino]methyl}-6-methylpyridin-3-yl)methyl dihydrogen phosphate: N202 (= N189), D230 (= D218), P232 (≠ A220), Y233 (= Y221), S260 (= S254), S262 (= S256), K263 (= K257), R270 (≠ G264)

Sites not aligning to the query:

binding (5-hydroxy-4-{[(7-hydroxy-6-oxo-2-phenyl-6,7-dihydro-2H-pyrazolo[3,4-b]pyridin-5-yl)amino]methyl}-6-methylpyridin-3-yl)methyl dihydrogen phosphate: 39, 40, 74, 117, 118, 142, 399

4ge9A Kynurenine aminotransferase ii inhibitors (see paper)
27% identity, 47% coverage: 179:380/431 of query aligns to 192:388/428 of 4ge9A

query
sites
4ge9A

K

K

G

F

I

L

W

Y

A

T

V

V

P

P

K

N

Y

G

S

N

N
|
N

P

P

T

T

G

G

D

N

I

-

Y

-

S

-

D

S

A

L

T

T

V

S

A

E

R

R

L

K

A

K

A

E

M

I

R

Y

T

E

G

L

A

A

R

R

-

K

-

Y

D

D

F

F

R

L

I

I

F

I

W

E

D
|
D

D

D

A
x
P

Y
|
Y

C

Y

V

F

H

L

H

Q

L

F

T

N

A

S

Q

G

R

R

N

V

A

P

V

T

A

F

N

L

I

S

L

M

E

D

E

V

C

D

A

G

R

R

A

V

G

I

N

R

Q

A

D

D

R

-

A

-

F

-

I

-

F

-

A

-

S
|
S

T

F

S
|
S

K

K

I

I

T

I

L

S

A

S

G

G

A

L

G
x
R

L

I

A

G

F

F

F

L

A

T

S

G

S

P

P

K

A

P

N

L

V

I

K

E

W

R

Y

V

L

I

S

L

S

H

A

I

S

Q

K

V

R

S

S

T

I
x
L

G

H

P

P

D

S

K

T

L

F

N

N

Q

Q

L

L

R

M

H

I

V

S

R

Q

F

L

L

L

R

H

D

E

-

W

-

G

A

E

E

E

G

G

I

F

N

M

A

A

H

H

M

V

D

D

A

R

H

V

R

I

A

D

L

F

I

Y

A

S

P

N

K

Q

F

K

A

D

A

A

V

I

I

L

E

A

A

A

L

A

D

D

R

K

H

W

L

L

A

-

G

-

T

T

G

G

A

L

A

A

N

E

W

W

S

H

V

V

P

P

Q

A

G

A

G

G

Y

M

F

F

-

L

-

W

I

I

S

K

V

V

E

K

A

G

S

I

A

N

G

D

T

V

A

K

R

E

R

L

V

I

V

E

E

E

L

K

A

A

K

V

A

K

A

M

G

G

V

V

A

L

L

M

T

L

P

P

A

G

G

N

A

A

T

F

W

Y

binding (4-{[(6-benzyl-1-hydroxy-7-methoxy-2-oxo-1,2-dihydroquinolin-3-yl)amino]methyl}-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate: N202 (= N189), D230 (= D218), P232 (≠ A220), Y233 (= Y221), S260 (= S254), S262 (= S256), R270 (≠ G264), L293 (≠ I287)

Sites not aligning to the query:

binding (4-{[(6-benzyl-1-hydroxy-7-methoxy-2-oxo-1,2-dihydroquinolin-3-yl)amino]methyl}-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate: 19, 20, 23, 39, 40, 74, 75, 77, 117, 118, 142, 399

4ge7A Kynurenine aminotransferase ii inhibitors (see paper)
27% identity, 47% coverage: 179:380/431 of query aligns to 192:388/428 of 4ge7A

query
sites
4ge7A

K

K

G

F

I

L

W

Y

A

T

V

V

P

P

K

N

Y

G

S

N

N
|
N

P

P

T

T

G

G

D

N

I

-

Y

-

S

-

D

S

A

L

T

T

V

S

A

E

R

R

L

K

A

K

A

E

M

I

R

Y

T

E

G

L

A

A

R

R

-

K

-

Y

D

D

F

F

R

L

I

I

F

I

W

E

D
|
D

D

D

A
x
P

Y
|
Y

C

Y

V

F

H

L

H

Q

L

F

T

N

A

S

Q

G

R

R

N

V

A

P

V

T

A

F

N

L

I

S

L

M

E

D

E

V

C

D

A

G

R

R

A

V

G

I

N

R

Q

A

D

D

R

-

A

-

F

-

I

-

F

-

A

-

S
|
S

T

F

S
|
S

K

K

I

I

T

I

L

S

A

S

G

G

A

L

G
x
R

L

I

A

G

F

F

F

L

A

T

S

G

S

P

P

K

A

P

N

L

V

I

K

E

W

R

Y

V

L

I

S

L

S

H

A

I

S

Q

K

V

R

S

S

T

I
x
L

G

H

P

P

D

S

K

T

L

F

N

N

Q

Q

L

L

R

M

H

I

V

S

R

Q

F

L

L

L

R

H

D

E

-

W

-

G

A

E

E

E

G

G

I

F

N

M

A

A

H

H

M

V

D

D

A

R

H

V

R

I

A

D

L

F

I

Y

A

S

P

N

K

Q

F

K

A

D

A

A

V

I

I

L

E

A

A

A

L

A

D

D

R

K

H

W

L

L

A

-

G

-

T

T

G

G

A

L

A

A

N

E

W

W

S

H

V

V

P

P

Q

A

G

A

G

G

Y

M

F

F

-

L

-

W

I

I

S

K

V

V

E

K

A

G

S

I

A

N

G

D

T

V

A

K

R

E

R

L

V

I

V

E

E

E

L

K

A

A

K

V

A

K

A

M

G

G

V

V

A

L

L

M

T

L

P

P

A

G

G

N

A

A

T

F

W

Y

binding (5-hydroxy-4-{[(1-hydroxy-2-oxo-6-phenoxy-1,2-dihydroquinolin-3-yl)amino]methyl}-6-methylpyridin-3-yl)methyl dihydrogen phosphate: N202 (= N189), D230 (= D218), P232 (≠ A220), Y233 (= Y221), S260 (= S254), S262 (= S256), R270 (≠ G264), L293 (≠ I287)

Sites not aligning to the query:

binding (5-hydroxy-4-{[(1-hydroxy-2-oxo-6-phenoxy-1,2-dihydroquinolin-3-yl)amino]methyl}-6-methylpyridin-3-yl)methyl dihydrogen phosphate: 19, 39, 74, 75, 77, 117, 118, 142, 399

4gdyB Kynurenine aminotransferase ii inhibitors
27% identity, 47% coverage: 179:380/431 of query aligns to 192:388/428 of 4gdyB

query
sites
4gdyB

K

K

G

F

I

L

W

Y

A

T

V

V

P

P

K

N

Y

G

S

N

N
|
N

P

P

T

T

G

G

D

N

I

-

Y

-

S

-

D

S

A

L

T

T

V

S

A

E

R

R

L

K

A

K

A

E

M

I

R

Y

T

E

G

L

A

A

R

R

-

K

-

Y

D

D

F

F

R

L

I

I

F

I

W

E

D
|
D

D

D

A
x
P

Y

Y

C

Y

V

F

H

L

H

Q

L

F

T

N

A

S

Q

G

R

R

N

V

A

P

V

T

A

F

N

L

I

S

L

M

E

D

E

V

C

D

A

G

R

R

A

V

G

I

N

R

Q

A

D

D

R

-

A

-

F

-

I

-

F

-

A

-

S
|
S

T

F

S
|
S

K

K

I

I

T

I

L

S

A

S

G

G

A

L

G
x
R

L

I

A

G

F

F

F

L

A

T

S

G

S

P

P

K

A

P

N

L

V

I

K

E

W

R

Y

V

L

I

S

L

S

H

A

I

S

Q

K

V

R

S

S

T

I

L

G

H

P

P

D

S

K

T

L

F

N

N

Q

Q

L

L

R

M

H

I

V

S

R

Q

F

L

L

L

R

H

D

E

-

W

-

G

A

E

E

E

G

G

I

F

N

M

A

A

H

H

M

V

D

D

A

R

H

V

R

I

A

D

L

F

I

Y

A

S

P

N

K

Q

F

K

A

D

A

A

V

I

I

L

E

A

A

A

L

A

D

D

R

K

H

W

L

L

A

-

G

-

T

T

G

G

A

L

A

A

N

E

W

W

S

H

V

V

P

P

Q

A

G

A

G

G

Y

M

F

F

-

L

-

W

I

I

S

K

V

V

E

K

A

G

S

I

A

N

G

D

T

V

A

K

R

E

R

L

V

I

V

E

E

E

L

K

A

A

K

V

A

K

A

M

G

G

V

V

A

L

L

M

T

L

P

P

A

G

G

N

A

A

T

F

W

Y

binding (5-hydroxy-6-methyl-4-{[(1-oxo-7-phenoxy-1,2-dihydro[1,2,4]triazolo[4,3-a]quinolin-4-yl)amino]methyl}pyridin-3-yl)methyl dihydrogen phosphate: N202 (= N189), D230 (= D218), P232 (≠ A220), S260 (= S254), S262 (= S256), R270 (≠ G264)

Sites not aligning to the query:

binding (5-hydroxy-6-methyl-4-{[(1-oxo-7-phenoxy-1,2-dihydro[1,2,4]triazolo[4,3-a]quinolin-4-yl)amino]methyl}pyridin-3-yl)methyl dihydrogen phosphate: 19, 33, 39, 74, 75, 116, 117, 118, 142, 399

Query Sequence

>WP_012592214.1 NCBI__GCF_000021745.1:WP_012592214.1
MTPIDQLSKAELAALRASVQANYDAFRAQGLKLDMTRGKPAPDQLDLANAMLSLMGNADY
RSEAGEDARNYGNLQGLAEARALIAPILGAPPEQIVVGDNSSLALMHDSLVYALTKGVPG
SARPWAKEESVAFICPAPGYDRHFAICEEYGVRMLPVRMTGEGPDMEEVERLAADPAVKG
IWAVPKYSNPTGDIYSDATVARLAAMRTGARDFRIFWDDAYCVHHLTAQRNAVANILEEC
ARAGNQDRAFIFASTSKITLAGAGLAFFASSPANVKWYLSSASKRSIGPDKLNQLRHVRF
LRDAEGINAHMDAHRALIAPKFAAVIEALDRHLAGTGAANWSVPQGGYFISVEASAGTAR
RVVELAKAAGVALTPAGATWPYGKDPDDSNLRLAPTFPTLAEVRAASEGIALCILLAALE
ARTAQAAESAA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory