SitesBLAST
Comparing WP_012592341.1 NCBI__GCF_000021745.1:WP_012592341.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
4bmsF Short chain alcohol dehydrogenase from ralstonia sp. Dsm 6428 in complex with NADPH
55% identity, 100% coverage: 1:244/244 of query aligns to 1:249/249 of 4bmsF
- active site: S137 (= S137), H147 (≠ F147), Y150 (= Y150), K154 (= K154), Q195 (≠ E190)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G13), N15 (≠ A15), S16 (≠ T16), I18 (= I18), R38 (= R38), R39 (= R39), A59 (≠ G59), D60 (≠ S60), V61 (= V61), N87 (= N87), S88 (≠ A88), G89 (= G89), V110 (= V110), S137 (= S137), Y150 (= Y150), K154 (= K154), G181 (= G181), I183 (≠ T183), T185 (= T185), I187 (vs. gap)
6ihhA Crystal structure of rasadh f12 from ralstonia.Sp in complex with NADPH and a6o
54% identity, 100% coverage: 1:244/244 of query aligns to 1:249/249 of 6ihhA
- binding (2R,3S)-2-ethyl-2-[(2E)-2-(6-methoxy-3,4-dihydro-2H-naphthalen-1-ylidene)ethyl]-3-oxidanyl-cyclopentan-1-one: S137 (= S137), H147 (≠ F147), Y150 (= Y150), L188 (vs. gap), L246 (= L241)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G13), N15 (≠ A15), S16 (≠ T16), G17 (= G17), I18 (= I18), R38 (= R38), R39 (= R39), D60 (≠ S60), V61 (= V61), N87 (= N87), S88 (≠ A88), G89 (= G89), V110 (= V110), T135 (= T135), S137 (= S137), Y150 (= Y150), K154 (= K154), P180 (= P180), G181 (= G181), A182 (= A182), I183 (≠ T183), T185 (= T185), S187 (vs. gap)
8ijgC Crystal structure of alcohol dehydrogenase m5 from burkholderia gladioli with NADP
54% identity, 99% coverage: 3:244/244 of query aligns to 3:250/250 of 8ijgC
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G13), T15 (≠ A15), S16 (≠ T16), I18 (= I18), R38 (= R38), R39 (= R39), D60 (≠ S60), V61 (= V61), N87 (= N87), G89 (= G89), R110 (≠ V110), N135 (≠ T135), A137 (≠ S137), Y150 (= Y150), K154 (= K154), P180 (= P180), G181 (= G181), T183 (= T183), T185 (= T185), G187 (vs. gap)
4esoB Crystal structure of a putative oxidoreductase protein from sinorhizobium meliloti 1021 in complex with NADP
37% identity, 98% coverage: 5:243/244 of query aligns to 4:246/251 of 4esoB
- active site: G16 (= G17), S136 (= S137), M146 (≠ F147), Y149 (= Y150), K153 (= K154)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G13), T14 (≠ A15), H15 (≠ T16), M17 (≠ I18), R37 (= R38), N38 (≠ R39), N41 (≠ A42), S58 (≠ G59), D59 (≠ S60), I60 (≠ V61), N86 (= N87), A87 (= A88), G88 (= G89), T134 (= T135), S136 (= S137), Y149 (= Y150), P179 (= P180), G180 (= G181), I182 (≠ T183), T184 (= T185), T186 (≠ L187), K187 (≠ A188), G188 (≠ K189)
4nbuB Crystal structure of fabg from bacillus sp (see paper)
36% identity, 99% coverage: 1:241/244 of query aligns to 2:242/244 of 4nbuB
- active site: G18 (= G17), N111 (= N111), S139 (= S137), Q149 (≠ F147), Y152 (= Y150), K156 (= K154)
- binding acetoacetyl-coenzyme a: D93 (≠ P93), K98 (≠ E98), S139 (= S137), N146 (≠ A144), V147 (≠ P145), Q149 (≠ F147), Y152 (= Y150), F184 (≠ A182), M189 (≠ L187), K200 (≠ A199)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G13), N17 (≠ T16), G18 (= G17), I19 (= I18), D38 (≠ G37), F39 (≠ R38), V59 (≠ G59), D60 (≠ S60), V61 (= V61), N87 (= N87), A88 (= A88), G89 (= G89), I90 (≠ V90), T137 (= T135), S139 (= S137), Y152 (= Y150), K156 (= K154), P182 (= P180), F184 (≠ A182), T185 (= T183), T187 (= T185), M189 (≠ L187)
1gegE Cryatal structure analysis of meso-2,3-butanediol dehydrogenase (see paper)
38% identity, 96% coverage: 7:241/244 of query aligns to 3:253/256 of 1gegE
- active site: G13 (= G17), S139 (= S137), Y152 (= Y150), K156 (= K154), V197 (≠ A188)
- binding alpha-D-glucopyranose: R63 (≠ L67), D64 (= D68), F67 (≠ Y71), E123 (≠ P124)
- binding nicotinamide-adenine-dinucleotide: G9 (= G13), Q12 (≠ T16), I14 (= I18), D33 (≠ R38), Y34 (≠ R39), V58 (≠ G59), D59 (≠ S60), V60 (= V61), N86 (= N87), A87 (= A88), I109 (≠ V110), S139 (= S137), Y152 (= Y150), K156 (= K154), P182 (= P180), V185 (≠ T183), T187 (= T185), M189 (≠ L187)
Q6WVP7 NADP-dependent (R)-specific alcohol dehydrogenase; (R)-specific ADH; Ketoreductase; KRED; EC 1.1.1.- from Lentilactobacillus kefiri (Lactobacillus kefiri) (see paper)
36% identity, 98% coverage: 3:241/244 of query aligns to 4:249/252 of Q6WVP7
Sites not aligning to the query:
Q48436 Diacetyl reductase [(S)-acetoin forming]; Acetoin(diacetyl) reductase; AR; Meso-2,3-butanediol dehydrogenase; EC 1.1.1.304 from Klebsiella pneumoniae (see paper)
38% identity, 96% coverage: 7:241/244 of query aligns to 3:253/256 of Q48436
- 6:33 (vs. 10:38, 34% identical) binding NAD(+)
- D59 (≠ S60) binding NAD(+)
- K156 (= K154) binding NAD(+)
1zk4A Structure of r-specific alcohol dehydrogenase (wildtype) from lactobacillus brevis in complex with acetophenone and NADP (see paper)
36% identity, 98% coverage: 2:241/244 of query aligns to 2:248/251 of 1zk4A
- active site: G17 (= G17), S142 (= S137), Y155 (= Y150), K159 (= K154)
- binding 1-phenylethanone: A93 (≠ G91), Y155 (= Y150), Y189 (≠ A182)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G13), T15 (≠ A15), L16 (≠ T16), I18 (= I18), T36 (≠ F36), G37 (= G37), R38 (= R38), H61 (≠ G59), D62 (≠ S60), N89 (= N87), A90 (= A88), G91 (= G89), I92 (≠ V90), Y155 (= Y150), G188 (= G181), I190 (≠ T183), T192 (= T185), L194 (= L187)
7ejhA Crystal structure of kred mutant-f147l/l153q/y190p/l199a/m205f/m206f and 2-hydroxyisoindoline-1,3-dione complex
36% identity, 98% coverage: 3:241/244 of query aligns to 5:250/253 of 7ejhA
- binding 2-oxidanylisoindole-1,3-dione: S144 (= S137), I145 (≠ S138), E146 (≠ A139), Y157 (= Y150), V197 (≠ A188), F207 (≠ K198)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G13), T17 (≠ A15), I20 (= I18), R40 (= R38), H41 (≠ R39), D64 (≠ S60), A65 (≠ V61), N91 (= N87), A92 (= A88), V114 (= V110), M142 (≠ T135), S144 (= S137), Y157 (= Y150), K161 (= K154), P189 (= P180), G190 (= G181), P191 (≠ A182), I192 (≠ T183), T194 (= T185), P195 (≠ E186), L196 (= L187)
7ejiB Crystal structure of kred f147l/l153q/y190p/l199a/m205f/m206f variant and methyl methacrylate complex
36% identity, 98% coverage: 3:241/244 of query aligns to 3:248/251 of 7ejiB
- binding methyl 2-methylprop-2-enoate: S142 (= S137), I143 (≠ S138), Y155 (= Y150), F205 (≠ K198)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G13), T15 (≠ A15), L16 (≠ T16), G17 (= G17), I18 (= I18), R38 (= R38), H39 (≠ R39), D62 (≠ S60), A63 (≠ V61), N89 (= N87), A90 (= A88), V112 (= V110), M140 (≠ T135), S142 (= S137), Y155 (= Y150), K159 (= K154), P187 (= P180), P189 (≠ A182), I190 (≠ T183), T192 (= T185), P193 (≠ E186), L194 (= L187)
6y0sAAA R-specific alcohol dehydrogenase (see paper)
36% identity, 98% coverage: 2:241/244 of query aligns to 2:248/251 of 6y0sAAA
1zk1A Structure of r-specific alcohol dehydrogenase (mutant g37d) from lactobacillus brevis in complex with phenylethanol and NAD (see paper)
36% identity, 98% coverage: 2:241/244 of query aligns to 2:248/251 of 1zk1A
- active site: G17 (= G17), S142 (= S137), Y155 (= Y150), K159 (= K154)
- binding 1-phenylethanone: A93 (≠ G91), N95 (≠ P93), Y155 (= Y150), Y189 (≠ A182)
- binding nicotinamide-adenine-dinucleotide: G13 (= G13), L16 (≠ T16), I18 (= I18), D37 (≠ G37), H61 (≠ G59), D62 (≠ S60), S63 (≠ V61), N89 (= N87), A90 (= A88), I92 (≠ V90), M140 (≠ T135), Y155 (= Y150), G188 (= G181), I190 (≠ T183), L194 (= L187)
1zjzA Structure of r-specific alcohol dehydrogenase (mutant g37d) from lactobacillus brevis in complex with phenylethanol and NAD (see paper)
36% identity, 98% coverage: 2:241/244 of query aligns to 2:248/251 of 1zjzA
- active site: G17 (= G17), S142 (= S137), Y155 (= Y150), K159 (= K154)
- binding nicotinamide-adenine-dinucleotide: G13 (= G13), L16 (≠ T16), I18 (= I18), D37 (≠ G37), D62 (≠ S60), N89 (= N87), A90 (= A88), G91 (= G89), I92 (≠ V90), Y155 (= Y150), G188 (= G181), I190 (≠ T183), L194 (= L187)
- binding (1r)-1-phenylethanol: A93 (≠ G91), N95 (≠ P93), L152 (≠ F147), Y155 (= Y150)
1zjyA Structure of r-specific alcohol dehydrogenase (mutant g37d) from lactobacillus brevis in complex with phenylethanol and nadh (see paper)
36% identity, 98% coverage: 2:241/244 of query aligns to 2:248/251 of 1zjyA
- active site: G17 (= G17), S142 (= S137), Y155 (= Y150), K159 (= K154)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G13 (= G13), L16 (≠ T16), G17 (= G17), I18 (= I18), D37 (≠ G37), D62 (≠ S60), N89 (= N87), A90 (= A88), G91 (= G89), I92 (≠ V90), Y155 (= Y150), G188 (= G181), I190 (≠ T183), L194 (= L187)
- binding (1r)-1-phenylethanol: A93 (≠ G91), N95 (≠ P93), L152 (≠ F147), Y155 (= Y150), Y189 (≠ A182)
3ay6B Crystal structure of bacillus megaterium glucose dehydrogenase 4 a258f mutant in complex with nadh and d-glucose (see paper)
36% identity, 99% coverage: 2:243/244 of query aligns to 9:258/267 of 3ay6B
- active site: G24 (= G17), S151 (= S137), Y164 (= Y150), K168 (= K154)
- binding beta-D-glucopyranose: E102 (≠ G91), S151 (= S137), H153 (≠ A139), W158 (≠ A144), Y164 (= Y150), N202 (≠ A188), K205 (≠ A191)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G20 (= G13), T23 (= T16), G24 (= G17), L25 (≠ I18), Y45 (≠ F36), D71 (≠ S60), V72 (= V61), N98 (= N87), A99 (= A88), G100 (= G89), V101 (= V90), M149 (≠ T135), S151 (= S137), Y164 (= Y150), K168 (= K154), P194 (= P180), G195 (= G181), M197 (≠ T183), T199 (= T185), P200 (≠ E186), I201 (≠ L187), N202 (≠ A188)
8cxaA Crystal structure of 3-oxoacyl-[acyl-carrier-protein] reductase from mycobacterium smegmatis with bound NAD
37% identity, 98% coverage: 3:241/244 of query aligns to 2:248/251 of 8cxaA
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), Q15 (≠ T16), G16 (= G17), I17 (= I18), D36 (vs. gap), V63 (= V61), N89 (= N87), A91 (≠ G89), S94 (= S92), I142 (≠ T135), S143 (≠ G136), S144 (= S137), Y157 (= Y150), K161 (= K154), P187 (= P180), H188 (≠ G181), I190 (≠ T183), I194 (≠ L187)
6ci9D Rmm microcompartment-associated aminopropanol dehydrogenase NADP + aminoacetone holo-structure (see paper)
37% identity, 98% coverage: 1:240/244 of query aligns to 4:248/259 of 6ci9D
- active site: G20 (= G17), S145 (= S137), Y159 (= Y150)
- binding 1-aminopropan-2-one: F97 (≠ G91), S145 (= S137), T147 (≠ A139), W156 (≠ F147), Y159 (= Y150), G190 (= G181)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G16 (= G13), S18 (≠ A15), G20 (= G17), I21 (= I18), G40 (= G37), R41 (= R38), N42 (≠ R39), D66 (≠ S60), V67 (= V61), N93 (= N87), G95 (= G89), T143 (= T135), S145 (= S137), Y159 (= Y150), K163 (= K154), P189 (= P180), N191 (≠ A182), I192 (≠ T183), T194 (= T185), G196 (≠ L187), L197 (≠ A188)
7v0hG Crystal structure of putative glucose 1-dehydrogenase from burkholderia cenocepacia in complex with NADP and a potential reaction product
36% identity, 99% coverage: 1:241/244 of query aligns to 6:252/253 of 7v0hG
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G18 (= G13), S20 (≠ A15), K21 (≠ T16), G22 (= G17), I23 (= I18), A43 (≠ G37), S44 (≠ R38), S45 (≠ R39), G68 (= G59), D69 (≠ S60), V70 (= V61), N96 (= N87), S97 (≠ A88), G98 (= G89), Y100 (≠ G91), I144 (≠ T135), S146 (= S137), Y159 (= Y150), K163 (= K154), P189 (= P180), G190 (= G181), M191 (≠ A182), I192 (≠ T183), T194 (= T185), G196 (≠ L187), T197 (≠ A188)
- binding (2R)-2-(hydroxymethyl)pentanedioic acid: S146 (= S137), Y159 (= Y150), M191 (≠ A182), I202 (vs. gap)
Q9BTZ2 Dehydrogenase/reductase SDR family member 4; NADPH-dependent carbonyl reductase; CR; NADPH-dependent retinol dehydrogenase/reductase; NRDR; humNRDR; Peroxisomal short-chain alcohol dehydrogenase; PSCD; SCAD-SRL; Short chain dehydrogenase/reductase family 25C member 2; Protein SDR25C2; Short-chain dehydrogenase/reductase family member 4; EC 1.1.1.184 from Homo sapiens (Human) (see 2 papers)
33% identity, 98% coverage: 1:240/244 of query aligns to 24:273/278 of Q9BTZ2
- S176 (≠ A144) Responsible for the stereoselective reduction of 3-ketosteroids into 3beta-hydroxysteroids and benzil into R-benzoin; mutation to F: Decreased reduction activity for benzil, isatin and retinal and increased activity for 5beta-Pregnane-3,20-dione and 5beta-Dihydrotestosterone. No change of stereoselectivity in 3-ketosteroids reduction and no change in 3beta-hydroxysteroid oxidation. Decreased reduction activity for isatin and increased activity for 5beta-Pregnane-3,20-dione, 5beta-Dihydrotestosterone, benzil and retinal; when associated with L-179. Change in stereoselective activity by the reduction of 5beta-Pregnane-3,20-dione predominantly to the 3alpha-hydroxysteroid; when associated with L-179. Switch from 3beta-hydroxysteroid to 3alpha-hydroxysteroid oxidation; when associated with L-179. Loss of cold catalytic inactivation; when associated with L-179 and N-195. Increased reduction activity for renital and oxidation activity for retinol; when associated with L-179 and N-195.
- F179 (= F147) Responsible for the stereoselective reduction of 3-ketosteroids into 3beta-hydroxysteroids and benzil into R-benzoin; mutation to L: Decreased reduction activity for isatin and increased activity for 5beta-Pregnane-3,20-dione, 5beta-Dihydrotestosterone, benzil and retinal; when associated with F-176. Change in stereoselective activity by the reduction of 5beta-Pregnane-3,20-dione predominantly to the 3alpha-hydroxysteroid; when associated with F-176. Switch from 3beta-hydroxysteroid to 3alpha-hydroxysteroid oxidation; when associated with F-176. Loss of cold catalytic inactivation; when associated with F-176 and N-195. Increased reduction activity for renital and oxidation activity for retinol; when associated with F-176 and N-195.
- T195 (= T163) mutation to N: Loss of cold catalytic inactivation. Loss of cold catalytic inactivation; when associated with F-176 and L-179. Switch in stereoselective activity from 3beta-hydroxysteroid to 3alpha-hydroxysteroid oxidation; when associated with F-176 and L-179. Increased reduction activity for renital and oxidation activity for retinol; when associated with F-176 and L-179.
Query Sequence
>WP_012592341.1 NCBI__GCF_000021745.1:WP_012592341.1
MTRLNGKTAVITGGATGIGLAAATRFIEEGAFVFIFGRRQEALDTAVAELGPNARAVKGS
VSNEADLDRLYAAVKAERGTLDIVFANAGVGSPLKLGEITAEHIDETFDVNVKGTIFTVQ
KALPLMGMGGSIILTGSSAGATGAPGFTAYSASKAAVRNLARTWAEDLKGTGIRVNVLSP
GATATELAKEALGEEGQKAFAVMTPLQRMADPSEIGAVAAFLASSDSSFMTASEVAVDGG
LAQI
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory