SitesBLAST

P37412 3-isopropylmalate dehydrogenase; 3-IPM-DH; Beta-IPM dehydrogenase; IMDH; EC 1.1.1.85 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
37% identity, 99% coverage: 1:354/359 of query aligns to 1:359/363 of P37412

query
sites
P37412

M

M

S

S

K

K

T

N

H

Y

K

H

I

I

A

A

I

V

V

L

G

P

G

G

D

D

G

G

T

I

G

G

P

P

E

E

V

V

V

M

A

A

E

Q

G

A

V

L

K

K

V

V

L

M

K

D

A

A

A

V

G

R

E

S

R

R

G

F

N

D

I

M

S

R

Y

I

E

T

F

T

V

S

E

H

F

Y

P

D

L

V

G

G

E

I

N

A

Y

I

M

D

K

N

T

H

G

G

D

H

L

P

L

L

T

P

Q

K

E

A

T

T

L

V

D

E

S

G

L

C

K

E

T

Q

M

A

D

D

A

A

I

I

Y

L

L

F

G

G

A

S

I

V

G

G

-

G

-

P

-

K

-

W

-

E

-

N

-

L

H

P

P

P

D

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V

S

K

Q

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P

G

-

I

-

L

-

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G

G

I

A

L

L

D

P

L

L

R

R

F

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H

L

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N

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R

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P

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A

K

K

L

L

Y

Y

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N

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L

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D

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A

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A

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P

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G

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M

L

F

Y

E

E

P

P

I

A

G

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S

A

A

P

P

K

D

Y

I

T

A

G

G

M

K

G

N

I

I

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A

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N

P

P

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A

A

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Y

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D

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A

A

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A

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A

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F

Y

V

V

D227 (= D225) binding Mn(2+)
D251 (= D249) binding Mn(2+)
D255 (= D253) binding Mn(2+)

3vkzA 3-isopropylmalate dehydrogenase from shewanella oneidensis mr-1 at atmospheric pressure (see paper)
38% identity, 98% coverage: 4:355/359 of query aligns to 2:361/364 of 3vkzA

query
sites
3vkzA

T

S

H

Y

K

Q

I

I

A

A

I

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V

L

G

A

G

G

D

D

G

G

T

I

G

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P

E

E

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M

A

A

E

E

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A

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K

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V

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L

K

K

A

A

A

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E

E

A

R

R

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F

N

G

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S

N

Y

I

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E

F

Y

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T

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E

F

Y

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D

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G

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D

K

N

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G

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A

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D

A

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I

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L

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G

A

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G

G

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G

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W

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G

-

I

-

L

-

E

E

K

R

G

G

I

A

L

L

D

P

L

L

R
|
R

F

G

S

H

L

F

D

E

Q

L

Y

F

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C

N

N

L

L

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R

P

P

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K

K

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P

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A

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F

D

D

F

V

V

L

V

C

V

V

R
|
R

E

E

N

L

T

T

E

G

G

G

L

I

Y
|
Y

A

F

G

G

A

K

G

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V

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L

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P

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K

G

G

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|
K

T

A

N

N

V

V

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F

C

A

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D

-

L

L

W

W

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Y

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V

A

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Y

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P

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D

I

V

E

E

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L

D

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Y

H

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D

A

N

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A

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T

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M

W

Q

M

L

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R

P

P

E

D

W

E

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D

D

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V

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C

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N

N

M

L

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G

D
|
D

I

I

I

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D

L

E

G

I

A

A

M

M

I

L

Q

T

G

G

G

S

M

M

G

G

I

L

A

L

A

S

G

S

G

A

N

S

I

M

N

N

P

S

E

T

G

G

V

F

S

G

M

L

F

F

E

E

P

P

I

A

G

G

S

S

A

A

P

P

K

D

Y

I

T

A

G

G

M

K

G

G

I

I

I

A

N

N

P

P

I

I

A

A

A

Q

I

I

G

L

A

S

G

A

Q

A

I

L

M

M

L

L

-

R

D

H

T

S

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G

K

E

Q

T

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A

A

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W

A

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R

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A

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I

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K

V

A

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L

R

N

D

S

-

G

-

Y

-

L

-

T

-

G

D

E

L

L

K

L

D

S

L

S

G

D

A

Q

G

R

K

H

M

K

G

A

F

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T

T

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E

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M

G

G

D

D

L

F

I

I

A

A

D

D

F

A

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V

K

K

active site: Y143 (= Y138), K193 (= K192), D225 (= D225), D249 (= D249), D253 (= D253)
binding calcium ion: D249 (= D249), D253 (= D253)
binding 3-isopropylmalic acid: R97 (= R95), R136 (= R131), Y143 (= Y138), D249 (= D249)

3vmkA 3-isopropylmalate dehydrogenase from shewanella benthica db21 mt-2 (see paper)
38% identity, 98% coverage: 4:354/359 of query aligns to 8:366/369 of 3vmkA

query
sites
3vmkA

T

S

H

Y

K

Q

I

I

A

A

I

V

V

L

G

A

G

G

D

D

G

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M

A

A

E

E

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A

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A

A

A

A

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K

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R

G

F

N

D

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Y

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E

E

F

Y

P

D

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E

A

N

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D

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N

T

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G

G

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E

E

A

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L

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L

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E

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A

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D

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Y

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G

P

P

-

K

-

W

-

E

D

H

V

L

K

P

P

P

G

N

-

D

I

Q

L

P

E

E

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R

G

G

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A

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D

P

L

L

R
|
R

F

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E

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C

N

N

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E

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-

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-

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D

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E

E

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F

D

D

F

I

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|
R

E

E

N

L

T

T

E

G

G

G

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Y
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Y

A

F

G

G

A

K

G

-

V

-

L

-

K

P

K

K

G

G

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L

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A

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F

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D

-

L

L

W

W

E

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R

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E

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A

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K

E

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Y

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P

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A

L

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A

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G

G

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M

G

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L

A

A

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G

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N

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G

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M

F

Y

E

E

P

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I

A

G

G

S

S

A

A

P

P

K

D

Y

I

T

A

G

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M

Q

G

G

I

I

I

A

N

N

P

P

I

V

A

A

A

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I

I

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A

S

G

A

Q

A

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M

L

L

L

-

R

D

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E

L

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D

A

A

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W

A

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A

G

A

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K

K

V

A

L

L

R

S

D

D

-

G

-

Y

-

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-

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E

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L

D

P

L

A

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G

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K

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D

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Y

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F

A

V

I

active site: Y149 (= Y138), K199 (= K192), D231 (= D225), D255 (= D249), D259 (= D253)
binding 3-isopropylmalic acid: R103 (= R95), R142 (= R131), Y149 (= Y138), K199 (= K192), N201 (= N194), D255 (= D249)

2d1cA Crystal structure of tt0538 protein from thermus thermophilus hb8
38% identity, 90% coverage: 7:329/359 of query aligns to 21:326/495 of 2d1cA

query
sites
2d1cA

I

I

A

T

I

V

V

I

G

P

G

G

D

D

G

G

T

I

G

G

P

P

E

E

V

C

V

V

A

E

E

A

G

T

V

L

K

K

V

V

L

L

K

E

A

A

G

-

E

-

R

K

G

A

N

P

I

L

S

A

Y

Y

E

E

F

V

V

R

E

E

F

-

P

-

L

-

G

A

G

G

E

A

N

S

Y

V

M

F

K

R

T

R

G

G

-

I

-

A

D

S

L

G

L

V

T

P

Q

Q

E

E

T

T

L

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D

E

S

S

L

I

K

R

T

K

M

T

D

R

A

V

I

V

Y

L

L

K

G

G

A
x
P

I
x
L

G
x
E

H
x
T

P

P

D

V

V

-

K

-

P

-

G

G

I

Y

L

G

E

E

K

K

G

S

I

A

L
x
N

L

V

D

T

L

L

R

R

F

K

S

L

L

F

D

E

Q

T

Y

Y

I

A

N

N

L

V

R

R

P

P

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K

R

L

E

Y

F

E

P

G

N

V

V

D

P

T

T

P

P

L

Y

K

A

D

G

K

R

G

G

P

-

E

-

D

-

I

I

D

D

F

L

V

V

R

R

E

E

N

N

T

V

E

E

G

D

L

L

Y
|
Y

A

A

G

G

A

-

G

-

V

-

L

I

K

E

K

H

G

M

T

Q

L

T

D

P

E

S

V

V

A

A

V

Q

Q

T

E

L

S

K

I

L

N

I

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K

K

G

G

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C

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Y

F

A

A

F

F

E

E

Y

L

C

A

Q

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K

A

R

E

N

G

N

R

K

K

G

-

K

-

V

V

T

H

L

C

C

A

G

T

K
|
K

T

S

N

N

V

I

L

M

T

K

F

L

A

A

F

E

D

G

L

T

W

L

E

K

R

R

V

A

F

F

Y

E

E

Q

V

V

A

A

K

Q

E

E

Y

Y

P

P

D

D

I

I

E

E

A

A

D

V

Y

H

A

I

H
x
I

V

V

D
|
D

A
x
N

T

A

C

A

M

H

W
x
Q

M

L

V

V

K

K

N

R

P

P

E

E

W

Q

F

F

D

E

V

V

I

I

V

V

T

T

D

T

N

N

M

M

F

N

G

G

D
|
D

I

I

I

L

T

S

D
|
D

L

L

G

T

A

S

M

G

I

L

Q

I

G

G

M
x
L

G
|
G

I

F

A

A

A

P

G

S

G

A

N

N

I

I

N

G

P

N

E

E

G

-

V

V

S

A

M

I

F

F

E

E

P

A

I

V

G
x
H

G
|
G

S
|
S

A
|
A

P

P

K
|
K

Y
|
Y

T

A

G

G

M

K

G

N

I

V

I
|
I

N
|
N

P

P

I

T

A

A

A

V

I

L

G

L

A

S

G

A

Q

V

I

M

M

M

L

L

D

R

T

Y

L

L

G

E

E

E

T

F

E

A

A

T

A

A

S

D

W

L

I

I

E

E

Q

N

G

A

I

L

K

Y

V

T

L

L

R

E

D

E

active site: Y143 (= Y138), K190 (= K192), D223 (= D225), D247 (= D249), D251 (= D253)
binding nadp nicotinamide-adenine-dinucleotide phosphate: P86 (≠ A75), L87 (≠ I76), E88 (≠ G77), T89 (≠ H78), N99 (≠ L91), I221 (≠ H223), N224 (≠ A226), Q228 (≠ W230), L260 (≠ M262), G261 (= G263), H279 (≠ G282), G280 (= G283), S281 (= S284), A282 (= A285), K284 (= K287), Y285 (= Y288), I291 (= I294), N292 (= N295)

Sites not aligning to the query:

binding nadp nicotinamide-adenine-dinucleotide phosphate: 333

P93832 3-isopropylmalate dehydrogenase 2, chloroplastic; 3-IPM-DH 2; AtIMDH2; AtIMDH3; IMDH 2; Beta-IPM dehydrogenase 2; Isopropylmalate dehydrogenase 2; AtIMD2; EC 1.1.1.85 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
37% identity, 92% coverage: 3:332/359 of query aligns to 41:372/405 of P93832

query
sites
P93832

K

K

T

R

H

Y

K

T

I

I

A

T

I

L

V

L

G

P

G

G

D

D

G

G

T

I

G

G

P

P

E

E

V

V

A

S

E

I

G

A

V

K

K

N

V

V

L

L

K

Q

A

Q

A

A

G

G

E

S

R

L

G

E

N

G

I

V

S

E

Y

F

E

N

F

F

V

R

E

E

F

M

P

P

L

I

G

G

E

A

N

A

Y

L

M

D

K

L

T

V

G

G

D

V

L

P

L

L

T

P

Q

E

E

E

T

T

L

I

D

S

S

A

L

A

K

K

T

E

M

S

D

D

A

A

I

V

Y

L

L

L

G

G

A

A

I
|
I

G
|
G

-
x
G

-
x
Y

-
x
K

-
x
W

-
x
D

-
x
N

H
x
E

P
x
K

D
x
H

V
x
L

K
x
R

P
|
P

G

-

I

-

L

-

E
|
E

K

K

G

G

I

-

L
|
L

D

Q

L

I

R
|
R

F

A

S

A

L

L

D

K

Q

V

Y

F

I

A

N

N

L

L

R
|
R

P

P

V

A

K

T

-

V

L

L

Y

P

E

Q

G

L

V

V

D

D

-

A

T

S

P

T

L

L

K

K

D

R

K

E

G

V

P

A

E

E

D

G

I

V

D

D

F

L

V

M

V

V

R
|
R

E

E

N

L

T

T

E

G

G

G

L

I

Y
|
Y

A

F

G

G

-

E

A

P

G

R

G

G

V

I

L

K

K

T

K

N

G

E

T

N

L

G

D

E

E

E

V

V

A

G

V

F

Q

N

E

T

S

E

I

V

N

Y

T

A

R

A

K

H

G

E

A

I

E

D

R

R

C

I

I

A

R

R

Y

V

A

A

F

F

E

E

Y

T

C

A

Q

R

K

K

R

R

N

R

N

G

K

K

K

-

G

-

K

-

V

-

T

-

L

L

C

C

G

S

-

V

-

D

K
|
K

T

A

N
|
N

V
|
V

L

L

T

E

F

A

A

S

F

I

D

-

L

L

W

W

E

R

R

K

V

R

F

V

Y

T

E

A

V

L

A

A

K

S

E

E

Y

Y

P

P

D

D

I

V

E

E

A

L

D

S

Y

H

A

M

H

Y

V

V

D
|
D

A
x
N

T

A

C

A

M

M

W

Q

M

L

V

V

K

R

N

D

P

P

E

K

W

Q

F

F

D

D

V

T

I

I

V

V

T

T

D

N

N

N

M

I

F

F

G

G

D
|
D

I

I

I

L

T

S

D
|
D

L

E

G

A

A

S

M

M

I

I

Q

T

G

G

G

S

M

I

G

G

I

M

A

L

A

P

G

S

G

A

N

S

I

L

N

S

P

D

E

S

G

G

V

P

S

G

M

L

F

F

E
|
E

P
|
P

I
|
I

G
x
H

G
|
G

S
|
S

A
|
A

P
|
P

K
x
D

Y
x
I

T
x
A

G
|
G

M
x
Q

G
x
D

I
x
K

I
x
A

N
|
N

P

P

I

L

A

A

A

T

I

I

G

L

A

S

G

A

Q

A

I

M

M

L

L

L

D

K

T

Y

-

G

L

L

G

G

E

E

T

E

E

K

A

A

S

K

W

R

I

I

E

E

Q

D

G

A

I

V

I

L

K

V

V

A

L

L

R

N

D

N

D

G

L

F

K

R

114:129 (vs. 76:87, 21% identical) binding NAD(+)
L132 (= L91) mutation to A: Reduced activity toward 3-isopropylmalate.
L133 (= L92) Confers substrate specificity; mutation to A: Reduced activity toward 3-isopropylmalate.; mutation to F: Enhanced activity toward 3-(2'-methylthio)-ethylmalate, but reduced catalytic efficiency with 3-isopropylmalate.
R136 (= R95) binding substrate; mutation to A: Loss of activity toward 3-isopropylmalate.; mutation to K: Reduced activity toward 3-isopropylmalate.
R146 (= R105) binding substrate; mutation to A: Reduced activity toward 3-isopropylmalate.; mutation to K: Reduced activity toward 3-isopropylmalate.
R174 (= R131) binding substrate; mutation to A: Loss of activity toward 3-isopropylmalate.; mutation to K: Reduced activity toward 3-isopropylmalate.
Y181 (= Y138) Important for catalysis; mutation Y->A,F,H: Reduced activity toward 3-isopropylmalate.
K232 (= K192) Important for catalysis; mutation to M: Loss of activity toward 3-isopropylmalate.
N234 (= N194) binding NAD(+); mutation N->A,D: Loss of activity toward 3-isopropylmalate.
V235 (= V195) mutation to A: Reduced activity toward 3-isopropylmalate.
D264 (= D225) binding Mg(2+); binding substrate; mutation to N: Loss of activity toward 3-isopropylmalate.
N265 (≠ A226) binding NAD(+)
D288 (= D249) binding Mg(2+); mutation to N: Loss of activity toward 3-isopropylmalate.
D292 (= D253) binding Mg(2+); mutation to N: Reduced activity toward 3-isopropylmalate.
318:334 (vs. 279:295, 53% identical) binding NAD(+)

2y41A Structure of isopropylmalate dehydrogenase from thermus thermophilus - complex with ipm and mn (see paper)
35% identity, 95% coverage: 4:344/359 of query aligns to 1:340/346 of 2y41A

query
sites
2y41A

T

S

H

M

K

K

I

V

A

A

I

V

V

L

G

P

G

G

D

D

G

G

T

I

G

G

P

P

E

E

V

V

V

T

A

E

E

A

G

A

V

L

K

K

V

V

L

L

K

R

A

A

A

L

G

D

E

E

R

A

G

E

N

G

I

L

S

G

Y

L

E

A

F

Y

V

E

E

V

F

F

P

P

L

F

G

G

E

A

N

A

Y

I

M

D

K

A

T

F

G

G

D

E

L

P

L

F

T

P

Q

E

E

P

T

T

L

R

D

K

S

G

L

V

K

E

T

E

M

A

D

E

A

A

I

V

Y

L

L

L

G

G

A

S

I

V

G

G

H

G

P

P

D

K

-

W

-

D

-

G

-

L

-

P

-

R

-

K

V

I

K

R

P

P

G

-

I

-

L

-

E

E

K

T

G

G

I

-

L

L

D

S

L

L

R
|
R

F

K

S

S

L

Q

D

D

Q

L

Y

F

I

A

N

N

L

L

R
|
R

P

P

V

A

K

K

L

V

Y

F

E

P

G

G

V

L

D

E

-

R

-

L

T

S

P

P

L

L

K

K

D

E

K

E

G

I

P

A

E

R

D

G

I

V

D

D

F

V

V

L

V

I

V

V

R
|
R

E

E

N

L

T

T

E

G

G

G

L

I

Y
|
Y

A

F

G

G

A

E

G

-

V

-

L

-

K

P

K

R

G

G

T

M

L

S

D

E

E

A

V

E

A

A

V

W

Q

N

E

T

S

E

I

R

N

Y

T

S

R

K

K

P

G

E

A

V

E

E

R

R

C

V

I

A

R

R

Y

V

A

A

F

F

E

E

Y

A

C

A

Q

R

K

K

R

R

N

R

N

-

K

-

G

-

K

K

K

H

V

V

T

V

L

S

C

V

G

D

K
|
K

T

A

N

N

V

V

L

L

T

E

F

V

A

G

F

-

D

E

L

F

W

W

E

R

R

K

V

T

F

V

Y

E

E

E

V

V

A

G

K

R

E

G

Y

Y

P

P

D

D

I

V

E

A

A

L

D

E

Y

H

A

Q

H

Y

V

V

D
|
D

A

A

T

M

C

A

M

M

W

H

M

L

V

V

K

R

N

S

P

P

E

A

W

R

F

F

D

D

V

V

I

V

V

V

T

T

D

G

N

N

M

I

F

F

G

G

D
|
D

I

I

I

L

T

S

D
|
D

L

L

G

A

A

S

M

V

I

L

Q

P

G

G

G

S

M

L

G

G

I

L

A

L

A

P

G

S

G

A

N

S

I

L

N

G

P

-

E

R

G

G

V

T

S

P

M

V

F

F

E

E

P

P

I

V

G

H

G

G

S

S

A

A

P

P

K

D

Y

I

T

A

G

G

M

K

G

G

I

I

I

A

N

N

P

P

I

T

A

A

I

I

G

L

A

S

G

A

Q

A

I

M

M

M

L

L

D

E

-

H

T

A

L

F

G

G

E

L

T

V

E

E

A

L

A

A

S

R

W

K

I

V

E

E

Q

D

G

A

I

V

I

A

K

K

-

A

V

L

L

L

R

E

D

T

D

P

L

P

K

P

D

D

L

L

G

G

-

G

-

S

A

A

G

G

K

T

M

E

G

A

F

F

S

T

T

A

S

T

active site: Y140 (= Y138), K186 (= K192), D218 (= D225), D242 (= D249), D246 (= D253)
binding 3-isopropylmalic acid: R95 (= R95), R105 (= R105), R133 (= R131), Y140 (= Y138), D242 (= D249)

2y42D Structure of isopropylmalate dehydrogenase from thermus thermophilus - complex with nadh and mn (see paper)
35% identity, 95% coverage: 4:344/359 of query aligns to 1:340/355 of 2y42D

query
sites
2y42D

T

S

H

M

K

K

I

V

A

A

I

V

V

L

G

P

G

G

D

D

G

G

T
x
I

G

G

P

P

E

E

V

V

V

T

A

E

E

A

G

A

V

L

K

K

V

V

L

L

K

R

A

A

A

L

G

D

E

E

R

A

G

E

N

G

I

L

S

G

Y

L

E

A

F

Y

V

E

E

V

F

F

P

P

L

F

G

G

E

A

N

A

Y

I

M

D

K

A

T

F

G

G

D

E

L

P

L

F

T

P

Q

E

E

P

T

T

L

R

D

K

S

G

L

V

K

E

T

E

M

A

D

E

A

A

I

V

Y

L

L

L

G

G

A

S

I

V

G

G

H

G

P

P

D

K

-

W

-
x
D

-

G

-

L

-

P

-

R

-

K

V

I

K

R

P

P

G

-

I

-

L

-

E

E

K

T

G

G

I

-

L

L

D

S

L

L

R

R

F

K

S

S

L

Q

D

D

Q

L

Y

F

I

A

N

N

L

L

R

R

P

P

V

A

K

K

L

V

Y

F

E

P

G

G

V

L

D

E

-

R

-

L

T

S

P

P

L

L

K

K

D

E

K

E

G

I

P

A

E

R

D

G

I

V

D

D

F

V

V

L

V

I

V

V

R

R

E

E

N

L

T

T

E

G

G

G

L

I

Y
|
Y

A

F

G

G

A

E

G

-

V

-

L

-

K

P

K

R

G

G

T

M

L

S

D

E

E

A

V

E

A

A

V

W

Q

N

E

T

S

E

I

R

N

Y

T

S

R

K

K

P

G

E

A

V

E

E

R

R

C

V

I

A

R

R

Y

V

A

A

F

F

E

E

Y

A

C

A

Q

R

K

K

R

R

N

R

N

-

K

-

G

-

K

K

K

H

V

V

T

V

L

S

C

V

G

D

K
|
K

T

A

N

N

V

V

L

L

T

E

F

V

A

G

F

-

D

E

L

F

W

W

E

R

R

K

V

T

F

V

Y

E

E

E

V

V

A

G

K

R

E

G

Y

Y

P

P

D

D

I

V

E

A

A

L

D

E

Y

H

A

Q

H

Y

V

V

D
|
D

A

A

T

M

C

A

M

M

W

H

M

L

V

V

K

R

N

S

P

P

E

A

W

R

F

F

D

D

V

V

I

V

V

V

T

T

D

G

N

N

M

I

F

F

G

G

D
|
D

I

I

I

L

T

S

D
|
D

L

L

G

A

A

S

M

V

I

L

Q

P

G

G

G

S

M

L

G

G

I

L

A

L

A

P

G

S

G

A

N

S

I

L

N

G

P

-

E

R

G

G

V

T

S

P

M

V

F

F

E

E

P

P

I

V

G
x
H

G
|
G

S

S

A
|
A

P

P

K
x
D

Y
x
I

T

A

G

G

M

K

G

G

I

I

I

A

N
|
N

P

P

I

T

A

A

I

I

G

L

A

S

G

A

Q

A

I

M

M

M

L

L

D

E

-

H

T

A

L

F

G

G

E

L

T

V

E

E

A

L

A

A

S

R

W

K

I

V

E

E

Q

D

G

A

I

V

I

A

K

K

-

A

V

L

L

L

R

E

D

T

D

P

L

P

K

P

D

D

L

L

G

G

-

G

-

S

A

A

G

G

K

T

M

E

G

A

F

F

S

T

T

A

S

T

active site: Y140 (= Y138), K186 (= K192), D218 (= D225), D242 (= D249), D246 (= D253)
binding manganese (ii) ion: D242 (= D249), D246 (= D253)
binding nicotinamide-adenine-dinucleotide: I12 (≠ T15), D79 (vs. gap), H274 (≠ G282), G275 (= G283), A277 (= A285), D279 (≠ K287), I280 (≠ Y288), N287 (= N295)

2ztwA Structure of 3-isopropylmalate dehydrogenase in complex with the inhibitor and NAD+ (see paper)
35% identity, 94% coverage: 6:344/359 of query aligns to 2:339/345 of 2ztwA

query
sites
2ztwA

K

K

I

V

A

A

I

V

V

L

G

P

G

G

D

D

G

G

T
x
I

G

G

P

P

E

E

V

V

V

T

A

E

E

A

G

A

V

L

K

K

V

V

L

L

K

R

A

A

A

L

G

D

E

E

R

A

G

E

N

G

I

L

S

G

Y

L

E

A

F

Y

V

E

E

V

F

F

P

P

L

F

G

G

E

A

N

A

Y

I

M

D

K

A

T

F

G

G

D

E

L

P

L

F

T

P

Q

E

E

P

T

T

L

R

D

K

S

G

L

V

K

E

T

E

M

A

D

E

A

A

I

V

Y

L

L

L

G

G

A

S

I

V

G

G

H

G

P

P

D

K

-

W

-

D

-

G

-

L

-

P

-

R

-

K

V

I

K

R

P

P

G

-

I

-

L

-

E

E

K

T

G

G

I

-

L

L

D

S

L

L

R

R

F

K

S

S

L

Q

D

D

Q

L

Y

F

I

A

N

N

L

L

R

R

P

P

V

A

K

K

L

V

Y

F

E

P

G

G

V

L

D

E

-

R

-

L

T

S

P

P

L

L

K

K

D

E

K

E

G

I

P

A

E

R

D

G

I

V

D

D

F

V

V

L

V

I

V

V

R

R

E

E

N

L

T

T

E

G

G

G

L

I

Y
|
Y

A

F

G

G

A

E

G

-

V

-

L

-

K

P

K

R

G

G

T

M

L

S

D

E

E

A

V

E

A

A

V

W

Q

N

E

T

S

E

I

R

N

Y

T

S

R

K

K

P

G

E

A

V

E

E

R

R

C

V

I

A

R

R

Y

V

A

A

F

F