SitesBLAST

M1 (= M1) modified: Initiator methionine, Removed
C2 (= C2) mutation C->A,S: Loss of glutamine-dependent activity but no effect on ammonia-dependent asparagine synthetase activity.
H30 (= H29) mutation to A: 4,5-fold decrease in glutamine affinity.
D34 (= D33) mutation D->N,E: Little effect on the kinetic properties.
H81 (≠ F82) mutation to A: 5-fold decrease in glutamine affinity.
A105 (≠ H107) mutation to H: Little effect on the kinetic properties.
E349 (= E367) mutation E->A,Q: Loss of glutamine- and ammonia-dependent synthetase activity, but still exhibits glutaminase activity.; mutation to D: 5-fold increase in affinity for aspartate when assaying both the glutamine- and ammonia-dependent synthetase reactions, and 2-fold decrease in kcat for these reactions. Modifies the product glutamate/asparagine stoichiometry.

P78753 Probable asparagine synthetase [glutamine-hydrolyzing]; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
31% identity, 58% coverage: 1:379/654 of query aligns to 1:368/557 of P78753

query
sites
P78753

M

M

C

C

G

G

F

I

L

L

A

A

V

V

F

H

H

H

G

V

G

A

P

E

R

D

P

I

L

E

A

A

P

F

S

K

T

P

I

K

L

A

A

L

G

H

L

L

S

S

A

K

-

Q

I

L

R

R

H

H

R

R

G

G

P

P

D

D

E

W

R

S

D

G

I

K

W

A

L

I

D

R

P

N

E

Q

A

-

G

-

V

T

A

I

L

L

G

C

H

H

T

E

R

R

L

L

S

A

I

I

I

V

G

G

L

V

S

E

N

S

G

G

R

A

Q

Q

P

P

I

L

A

V

S

S

Q

D

D

D

G

G

A

K

L

L

Q

V

L

L

I

T

V

V

N

N

G

G

E

E

F

I

Y

Y

D

N

F

H

E

L

R

K

I

L

R

R

A

E

E

N

L

L

Q

K

A

G

R

-

G

N

C

Y

V

K

F

F

R

K

T

T

Q

Y

S

S

D

D

S

C

E

E

I

V

A

I

L

L

H

Y

L

L

Y

Y

R

R

E

E

F

H

G

G

T

P

A

A

G

C

L

A

G

N

R

M

L

L

R

D

G

G

E

M

F

F

A

S

L

W

V

V

L

L

Y

Y

D

D

A

Q

V

D

E

K

R

D

Q

K

L

V

V

V

V

A

M

A

R

R

D

D

R

P

F

I

G

G

I

I

K

T

P

T

L

L

-

Y

-

Q

-

G

F

F

V

S

A

S

E

D

H

S

D

P

G

D

A

T

L

A

W

Y

V

F

G

A

S

S

E

E

I

L

K

K

A

A

L

L

-

H

-

P

V

V

A

C

A

D

G

K

V

I

P

I

A

A

F

F

W

-

D

-

L

-

D

-

A

-

Y

-

V

-

S

P

R

P

G

G

F

H

Y

Y

L

Y

G

D

N

S

R

E

T

T

L

K

F

-

Q

Q

G

T

I

V

R

R

S

Y

L

F

A

K

P

P

G

-

H

-

L

-

I

-

A

-

S

-

P

-

G

-

H

-

I

-

R

-

E

-

R

-

A

S

Y

W

W

W

D

D

L

E

D

N

F

K

P

I

A

P

A

S

A

N

A

P

L

V

D

D

S

-

A

-

I

-

D

-

E

-

A

-

E

-

A

-

V

Y

E

K

G

L

L

L

R

R

A

E

E

T

I

L

L

E

E

A

A

S

V

V

G

R

L

K

R

R

L

L

R

M

A

A

D

E

V

V

P

P

I

Y

A

G

V

V

Y

L

L

L

S

S

G

G

I

L

D

D

S

S

A

L

M

I

L

A

G

S

C

I

A

A

T

A

R

R

-

E

-

T

-

E

-

K

-

L

-

A

-

N

-

S

-

T

-

S

-

Q

-

S

-

E

-

A

-

R

-

T

L

I

R

T

G

A

T

W

P

P

-

K

L

L

D

H

A

S

F

F

T

A

L

I

S

G

F

L

T

P

D

G

D

S

G

P

D

D

Y

L

D

L

E

A

R

A

R

R

F

-

A

-

E

K

A

V

A

A

S

D

F

F

N

L

G

H

A

T

R

F

F

H

H

H

P

E

I

H

P

T

V

F

T

T

Q

I

D

D

D

E

L

G

A

L

D

D

D

A

F

L

E

R

E

D

A

V

L

I

W

Y

H

H

N

L

E

E

V

T

P

Y

F

D

F

V

N

T

A

T

-

I

-

R

H

A

G

S

V

T

A

P

K

M

Y

Y

R

L

L

L

S

S

R

R

V

K

V

I

A

K

Q

A

A

Q

R

G

F

V

K

K

V

M

V

V

I

L

T

S

G

G

E

E

G

G

A

S

D

D

E

E

I

I

F

F

A

G

G

G

Y

Y

P

L

H

Y

F

F

Sites not aligning to the query:

391 modified: Phosphoserine
489 modified: Phosphoserine

P08243 Asparagine synthetase [glutamine-hydrolyzing]; Cell cycle control protein TS11; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Homo sapiens (Human) (see 7 papers)
29% identity, 58% coverage: 1:381/654 of query aligns to 1:379/561 of P08243

query
sites
P08243

M

M

C
|
C

G

G

F

I

L

W

A
|
A

V

L

F

F

H

-

G

G

G

S

P

D

R

D

P

C

L

L

A

S

P

V

S

Q

T

C

I

L

L

S

A

A

G

-

L

-

S

M

A

K

I

I

R

A

H

H

R

R

G

G

P

P

D

D

E

A

-

F

R

R

D

F

I

E

W

N

L

V

D

N

P

G

E

Y

A

T

G

N

V

C

A

C

L

F

G

G

H

F

T

H

R

R

L

L

S

A

I

V

G

D

L

P

S

L

N

F

G

G

R

M

Q

Q

P

P

I

I

-

R

A

V

S

K

Q

K

D

Y

G

P

A

Y

L

L

Q

W

L

L

I

C

V

Y

N

N

G

G

E

E

F

I

Y

Y

D

N

F

H

E

K

R

K

I

M

R

Q

A

Q

E

H

L

F

Q

E

A

F

R

E

G

-

C

-

V

-

F

Y

R

Q

T

T

Q

K

S

V

D

D

S

G

E

E

I

I

A

I

L

L

H

H

L

L

Y

Y

R

D

E

K

F

G

G

G

T

I

-

E

A

Q

G

T

L

I

G

C

R

M

L

L

R

D

G

G

E

V

F

F

A

A

L

F

V

V

L

L

Y

L

D

D

A

T

V

A

E

N

R

K

Q

K

L

V

V

F

V

L

M

G

R

R

D

D

R

T

F

Y

G

G

I

V

K

R

P

P

L

L

F

F

V

K

A

A

-

M

E

T

H

E

D

D

G

G

A

F

L

L

W

A

V

V

G

C

S

S

E

E

I

A

K

K

A

G

L

L

V

V

A

T

A

L

G

K

V

H

P

S

A

A

F

-

W

-

D

-

L

-

D

-

A

-

Y

-

V

-

S

-

R

-

G

-

F

-

Y

-

L

-

G

-

N

-

R

-

T

T

L

P

F

F

Q

L

G

K

I

V

R

E

S

P

L

F

A

L

P

P

G

G

H

H

-

Y

-

E

-

V

L

L

L

D

I

L

A

K

S

P

P

N

G

G

H

K

I

V

R

A

E

S

-

V

-

E

-

M

-

V

R

K

A

Y

Y

H

W

H

D

C

L

R

D

D

F
x
V

P

P

A

L

A

H

A

A

A

L

L

Y

D

D

S

N

A

V

A

E

I

K

-

L

-

F

-

P

-

G

D

F

E

E

A

I

E

E

A

T

V

V

E

K

-

N

G

N

L

L

R

R

A

I

E

L

I

F

L

N

E

N

A

A

V

V

G

K

L

K

R

R

L

L

R

M

A

T

D

D

V

R

P

R

I

I

A

G

V

C

Y

L

L

L

S

S

G

G

I

L

D

D

S

S

A

-

M

-

L

L

G

V

C

A

A

A

T

T

R

L

L

L

R

K

G

Q

T

L

-

K

-

E

-

A

-

Q

-

V

-

Q

-

Y

P

P

L

L

D

Q

A

T

F

F

T

A

L

I

S

G

F

M

T

E

D

D

D

S

G

P

D

D

Y

L

D

L

E

A

R

A

R

R

F

-

A

-

E

K

A

V

A

A

S

D

F

H

N

I

G

G

A

S

R

E

F

H

H

Y

P

E

I

V

P

L

V

F

T

N

Q

S

D

E

L

G

A

I

D

Q

D

A

F

L

E

D

E

E

A

V

L

I

W

F

H

S

N

L

E

E

V

T

P

Y

F

D

F

I

N

T

A

T

-

V

-

R

H

A

G

S

V

V

A

G

K

M

Y

Y

R

L

L

I

S

S

R

K

V

Y

V

I

A

R

Q

K

A

N

R

T

F

D

K

S

V

V

I

I

-
x
F

T

S

G

G

E

E

G

G

A

S

D

D

E

E

I

L

F

T

A

Q

G

G

Y

Y

P

I

H

Y

F

F

R

H

R

K

C2 (= C2) active site, For GATase activity; mutation to A: Loss of the glutamine-dependent asparagine synthetase activity, while the ammonia-dependent activity remained unaffected.
A6 (= A6) to E: in ASNSD; dramatic reduction in protein abundance; dbSNP:rs398122975
V210 (≠ F222) to E: in dbSNP:rs1049674
F362 (vs. gap) to V: in ASNSD; dramatic reduction in protein abundance; dbSNP:rs398122973

Sites not aligning to the query:

550 R → C: in ASNSD; increases level of protein abundance; dbSNP:rs398122974

6gq3A Human asparagine synthetase (asns) in complex with 6-diazo-5-oxo-l- norleucine (don) at 1.85 a resolution (see paper)
29% identity, 58% coverage: 2:381/654 of query aligns to 1:366/509 of 6gq3A

query
sites
6gq3A

C
|
C

G

G

F

I

L
x
W

A

A

V

L

F

F

H

-

G

G

G

S

P

D

R

D

P

C

L

L

A

S

P

V

S

Q

T

C

I

L

L

S

A

A

G

-

L

-

S

M

A

K

I

I

R

A

H

H

R

R

G

G

P

P

D

D

E

A

-

F

R

R

D

F

I

E

W

N

L

V

D

N

P

G

E

Y

A

T

G

N

V

C

A

C

L

F

G

G

H

F

T

H

R
|
R

L
|
L

S

A

I
x
V

G

D

L

P

S

L

N

F

G

G

R

M

Q

Q

P

P

I

I

-

R

A

V

S

K

Q

K

D

Y

G

P

A

Y

L

L

Q

W

L

L

I

C

V
x
Y

N
|
N

G
|
G

E
|
E

F

I

Y

Y

D

N

F

H

E

K

R

K

I

M

R

Q

A

Q

E

H

L

F

Q

E

A

F

R

E

G

-

C

-

V

-

F

Y

R

Q

T

T

Q

K

S
x
V

D
|
D

S

G

E

E

I

I

A

I

L

L

H

H

L

L

Y

Y

R

D

E

K

F

G

G

G

T

I

-

E

A

Q

G

T

L

I

G

C

R

M

L

L

R

D

G

G

E

V

F

F

A

A

L

F

V

V

L

L

Y

L

D

D

A

T

V

A

E

N

R

K

Q

K

L

V

V

F

V

L

M

G

R

R

D

D

R

T

F

Y

G

G

I

V

K

R

P

P

L

L

F

F

V

K

A

A

-

M

E

T

H

E

D

D

G

G

A

F

L

L

W

A

V

V

G

C

S

S

E

E

I

A

K

K

A

G

L

L

V

V

A

T

A

L

G

K

V

H

P

S

A

A

F

-

W

-

D

-

L

-

D

-

A

-

Y

-

V

-

S

-

R

-

G

-

F

-

Y

-

L

-

G

-

N

-

R

-

T

T

L

P

F

F

Q

L

G

K

I

V

R

E

S

P

L

F

A

L

P

P

G

G

H

H

L

Y

-

E

-

V

-

L

-

D

-

L

-

K

-

P

-

N

-

G

L

K

I

V

A

A

S

S

P

V

G

E

H

M

I

V

R

K

E

Y

R

H

A

H

Y

C

W

R

D

D

L

V

D

-

F

F

P

P

A

G

A

F

A

-

L

-

D

-

S

-

A

-

I

-

D

-

E

E

A

I

E

E

A

T

V

V

E

K

-

N

G

N

L

L

R

R

A

I

E

L

I

F

L

N

E

N

A

A

V

V

G

K

L

K

R

R

L

L

R

M

A

T

D

D

V

R

P

R

I

I

A

G

V

C

Y

L

L

L

S

S

G

G

I

L

D

D

S

S

A

-

M

-

L

L

G

V

C

A

A

A

T

T

R

L

L

L

R

K

G

Q

T

L

-

K

-

E

-

A

-

Q

-

V

-

Q

-

Y

P

P

L

L

D

Q

A

T

F

F

T

A

L

I

S

G

F

M

T

E

D

D

D

S

G

P

D

D

Y

L

D

L

E

A

R

A

R

R

F

-

A

-

E

K

A

V

A

A

S

D

F

H

N

I

G

G

A

S

R

E

F

H

H

Y

P

E

I

V

P

L

V

F

T

N

Q

S

D

E

L

G

A

I

D

Q

D

A

F

L

E

D

E

E

A

V

L

I

W

F

H

S

N

L

E

E

V

T

P

Y

F

D

F

I

N
x
T

A

T

-

V

-
x
R

H

A

G

S

V

V

A

G

K

M

Y

Y

R

L

L

I

S

S

R

K

V

Y

V

I

A

R

Q

K

A

N

R

T

F

D

K

S

V

V

I

I

-

F

T

S

G

G

E

E

G

G

A

S

D

D

E

E

I

L

F

T

A

Q

G

G

Y

Y

P

I

H

Y

F

F

R

H

R

K

active site: W4 (≠ L5), L49 (= L52), N74 (= N76), G75 (= G77), T324 (≠ N342), R327 (vs. gap)
binding 5-oxo-l-norleucine: C1 (= C2), R48 (= R51), V51 (≠ I54), V52 (≠ I55), Y73 (≠ V75), N74 (= N76), G75 (= G77), E76 (= E78), V95 (≠ S100), D96 (= D101)

Q9STG9 Amidophosphoribosyltransferase 2, chloroplastic; AtATase2; AtPURF2; PRPP2; Glutamine phosphoribosylpyrophosphate amidotransferase 2; AtGPRAT2; Protein CHLOROPLAST IMPORT APPARATUS 1; Protein DIFFERENTIAL DEVELOPMENT OF VASCULAR ASSOCIATED CELLS; EC 2.4.2.14 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
32% identity, 19% coverage: 45:166/654 of query aligns to 153:285/561 of Q9STG9

query
sites
Q9STG9

V

I

A

A

L

I

G

G

H

H

T

V

R

R

L

Y

S

S

I

T

I

A

G

G

L

S

S

S

-

M

-

L

N

K

G

N

R

V

Q

Q

P

P

I

F

A

V

S

A

-

G

-

Y

Q

R

D

F

G

G

A

S

L

V

Q

G

L

V

I

A

V
x
H

N

N

G

G

E

N

F

L

Y

V

D

N

F

Y

E

T

R

K

I

L

R

R

A

A

E

D

L

L

Q

E

A

E

R

N

G

G

C

S

V

I

F

F

R

N

T

T

Q

S

S

S

D

D

S

T

E

E

I

V

A

V

L

L

H

H

L

L

Y

I

-

A

-

I

-

S

-

K

-

A

R

R

E

P

F

F

G

F

-

M

-

R

-

I

T

V

A

D

G

A

L

C

G

E

R

K

L

L

R

Q

G

G

E

A

F

Y

A

S

L

M

V

V

L

F

Y

-

D

-

A

V

V

T

E

E

R

D

Q

K

L

L

V

V

V

A

M

V

R

R

D

D

R

P

F

H

G

G

I

F

K
x
R

P
|
P

L

L

F

V

V

M

A

G

-

R

E

R

H

S

D

N

G

G

A

A

L

V

W

V

V

F

G

A

S

S

E

E

I

T

K

C

A

A

L

L

H187 (≠ V75) mutation to T: In cia1-2; small plants with white leaves showing an irregular mosaic of green sectors.
R264 (≠ K146) mutation to K: Strong resistance to the bleaching herbicides DAS073 and DAS734.
P265 (= P147) mutation to S: Low resistance to the bleaching herbicides DAS073 and DAS734; when associated with F-494.

Sites not aligning to the query:

371 G→S: Low resistance to the bleaching herbicides DAS073 and DAS734.
476 P→S: Resistance to the bleaching herbicides DAS073 and DAS734.
494 Y→F: Low resistance to the bleaching herbicides DAS073 and DAS734; when associated with S-265.

6lbpA Structure of the glutamine phosphoribosylpyrophosphate amidotransferase from arabidopsis thaliana (see paper)
32% identity, 19% coverage: 45:166/654 of query aligns to 67:199/460 of 6lbpA

query
sites
6lbpA

V

I

A

A

L

I

G

G

H

H

T

V

R

R

L

Y

S

S

I

T

I

A

G

G

L

S

S

S

-

M

-

L

N

K

G

N

R

V

Q

Q

P

P

I

F

A

V

S

A

-

G

-

Y

Q

R

D

F

G

G

A

S

L

V

Q

G

L

V

I

A

V

H

N
|
N

G
|
G

E

N

F

L

Y

V

D

N

F

Y

E

T

R

K

I

L

R

R

A

A

E

D

L

L

Q

E

A

E

R

N

G

G

C

S

V

I

F

F

R

N

T

T

Q

S

S

S

D

D

S

T

E

E

I

V

A

V

L

L

H

H

L

L

Y

I

-

A

-

I

-

S

-

K

-

A

R

R

E

P

F

F

G

F

-

M

-

R

-

I

T

V

A

D

G

A

L

C

G

E

R

K

L

L

R

Q

G

G

E

A

F

Y

A

S

L

M

V

V

L

F

Y

-

D

-

A

V

V

T

E

E

R

D

Q

K

L

L

V

V

V

A

M

V

R

R

D

D

R

P

F

H

G

G

I

F

K

R

P

P

L

L

F

V

V

M

A

G

-

R

E

R

H

S

D

N

G

G

A

A

L

V

W

V

V

F

G

A

S

S

E

E

I

T

K

C

A

A

L

L

active site: N102 (= N76), G103 (= G77)

Sites not aligning to the query:

active site: 1, 27, 243, 301, 306, 316, 424
binding iron/sulfur cluster: 237, 239, 383, 385, 434, 436, 437

1ao0A Glutamine phosphoribosylpyrophosphate (prpp) amidotransferase from b. Subtilis complexed with adp and gmp (see paper)
27% identity, 20% coverage: 46:178/654 of query aligns to 67:206/455 of 1ao0A

query
sites
1ao0A

A

A

L

I

G

G

H

H

T

V

R

R

L

Y

S

A

I

T

I

-

G

G

L

Y

S

E

N

N

G

-

R

V

Q

Q

P

P

I

L

-

L

-

F

-

R

A

S

S

Q

Q

N

D

N

G

G

A

S

L

L

Q

A

L

L

I

A

V

H

N
|
N

G
|
G

E

N

F

L

Y

V

D

N

F

A

E

T

R

Q

I

L

R

K

A

Q

E

Q

L

L

Q

E

A

N

R

Q

G

G

C

S

V

I

F

F

R

Q

T

T

Q

S

S

S

D

D

S

T

E

E

I

V

A

L

L

A

H

H

L

L

Y

I

R

K

E

R

F

S

G

G

-

H

-

F

-

T

-

L

-

K

-

D

-

Q

-

I

T

K

A

N

G

S

L

L

G

S

R

M

L

L

R

K

G

G

E

A

F

Y

A

A

L

F

V

L

L

I

Y

M

D

-

A

-

V

T

E

E

R

T

Q

E

L

M

V

I

V

V

M

A

R

L

D

D

R

P

F

N

G

G

I

L

K

R

P

P

L

L

F

S

V

I

A

G

E

M

H

M

D

G

G

D

A

A

L

Y

W

V

V

V

G

A

S

S

E

E

I

T

K

C

A

A

L

F

V

D

A

V

G

G

V

A

P

T

A

Y

F

L

W

R

D

E

L

V

D

E

active site: N98 (= N76), G99 (= G77)

Sites not aligning to the query:

active site: 1, 27, 238, 296, 301, 311, 419
binding guanosine-5'-monophosphate: 234, 238, 279, 341, 342, 343, 345, 346, 347, 348, 349
binding adenosine-5'-diphosphate: 25, 238, 240, 241, 242, 277, 278, 279, 300, 301, 301, 303
binding magnesium ion: 279, 341, 342
binding iron/sulfur cluster: 232, 233, 234, 378, 380, 433, 436

1jgtB Crystal structure of beta-lactam synthetase (see paper)
24% identity, 40% coverage: 74:337/654 of query aligns to 71:314/500 of 1jgtB

query
sites
1jgtB

I

V

V

L

N
x
A

G
|
G

E

E

F

I

Y

Y

D

N

F

R

E

D

R

E

I

L

R

L

A

S

E

V

L

L

Q

P

A

A

R

G

G

P

C

A

V

-

F

-

R

-

T

P

Q

E

S

G

D

D

S

A

E

E

I

L

A

V

L

L

H

R

L

L

Y

L

R

E

E

R

F

Y

G

D

T

L

A

H

G

A

L

F

G

R

R

L

L

V

R

N

G

G

E

R

F

F

A

A

L

T

V

V

L

V

Y

R

D

T

A

G

V

-

E

-

R

D

Q

R

L

V

V

L

M

A

R

T

D

D

R

H

F

A

G

G

I

S

K

V

P

P

L

L

F

Y

V

T

A

C

E

V

H

A

D

P

G

G

A

E

L

V

W

R

V

A

G

S

S

T

E

E

I

A

K

K

A

A

L

L

V

A

A

A

A

H

G

R

V

D

P

P

A

-

F

-

W

-

D

-

L

-

D

-

A

-

Y

-

V

-

S

-

R

K

G

G

F

F

Y

P

L

L

G

A

N

D

R

A

T

R

L

R

F

V

Q

A

G

G

I

L

R

T

S

G

L

V

A

Y

P

-

G

-

H

-

L

-

I

-

A

-

S

-

P

-

G

-

H

Q

I

V

R

P

E

A

R

G

A

A

Y

V

W

M

D

D

L

I

D

D

F

L

P

G

A

S

A

G

A

T

A

A

-

V

-

T

-

H

-

R

-

T

-

W

-

T

-

P

-

G

L

L

D

S

S

R

A

R

A

I

I

L

D

P

E

E

A

G

E

E

A

A

V

V

E

A

G

A

L

V

R

R

A

A

E

A

I

L

L

E

E

K

A

A

V

V

G

A

L

Q

R

R

L

V

R

T

-

P

A

G

D

D

V

T

P

P

I

L

A

-

V

V

Y
x
V

L
|
L

S
|
S

G

G

G
|
G

I
|
I

D
|
D

S
|
S

S

S

A

G

M

V

L

A

G

A

C

C

A

A

T

H

R

R

L

A

R

A

G

G

T

E

P

-

L

L

D

D

A

T

F

V

T
x
S

L
x
M

S

G

F

T

T

D

D

T

D

S

G

N

D

E

Y

F

D

R

E

E

R

A

R

R

F

A

A

V

E

D

A

H

A

L

S

R

F

-

N

-

G

-

A

T

R

R

F

H

H

R

P

E

I

I

P

T

V

I

T

P

Q

T

D

T

D

E

L

L

A

L

D

A

D

Q

F

L

E

P

E

Y

A

A

L

V

W

W

H

A

N

S

E

E

active site: A73 (≠ N76), G74 (= G77)
binding diphosphomethylphosphonic acid adenosyl ester: V244 (≠ Y263), L245 (= L264), S246 (= S265), G248 (= G267), I249 (= I268), D250 (= D269), S251 (= S270), S269 (≠ T289), M270 (≠ L290)
binding magnesium ion: D250 (= D269)

Sites not aligning to the query:

active site: 319, 345, 379, 440
binding diphosphomethylphosphonic acid adenosyl ester: 327, 344, 345, 348, 420, 440
binding n2-(carboxyethyl)-l-arginine: 323, 345, 346, 348, 349, 354, 370, 379
binding magnesium ion: 348

1gph1 Structure of the allosteric regulatory enzyme of purine biosynthesis (see paper)
26% identity, 20% coverage: 46:178/654 of query aligns to 67:210/465 of 1gph1

query
sites
1gph1

A

A

L

I

G

G

H

H

T

V

R

R

L

Y

S

A

I

T

I

A

G

G

L

G

S

G

-

G

-

Y

N

E

G

N

R

V

Q

Q

P

P

I

L

-

L

-

F

-

R

A

S

S

Q

Q

N

D

N

G

G

A

S

L

L

Q

A

L

L

I

A

V

H

N

N

G

G

E

N

F

L

Y

V

D

N

F

A

E

T

R

Q

I

L

R

K

A

Q

E

Q

L

L

Q

E

A

N

R

Q

G

G

C

S

V

I

F

F

R

Q

T

T

Q

S

S

S

D

D

S

T

E

E

I

V

A

L

L

A

H

H

L

L

Y

I

R

K

E

R

F

S

G

G

-

H

-

F

-

T

-

L

-

K

-

D

-

Q

-

I

T

K

A

N

G

S

L

L

G

S

R

M

L

L

R

K

G

G

E

A

F

Y

A

A

L

F

V

L

L

I

Y

M

D

-

A

-

V

T

E

E

R

T

Q

E

L

M

V

I

V

V

M

A

R

L

D

D

R

P

F

N

G

G

I

L

K

R

P

P

L

L

F

S

V

I

A

G

E

M

H

M

D

G

G

D

A

A

L

Y

W

V

V

V

G

A

S

S

E

E

I

T

K

C

A

A

L

F

V

D

A

V

G

G

V

A

P

T

A

Y

F

L

W

R

D

E

L

V

D

E

Sites not aligning to the query:

active site: 300, 305, 315, 423
binding adenosine monophosphate: 242, 242, 244, 245, 246, 282, 283, 283, 304, 305, 307, 345, 346, 347, 349, 350, 353, 388
binding iron/sulfur cluster: 236, 237, 382, 384, 388, 437, 440

P00497 Amidophosphoribosyltransferase; ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase; EC 2.4.2.14 from Bacillus subtilis (strain 168) (see 5 papers)
26% identity, 20% coverage: 46:178/654 of query aligns to 78:221/476 of P00497

query
sites
P00497

A

A

L

I

G

G

H

H

T

V

R

R

L

Y

S

A

I

T

I

A

G

G

L

G

S

G

-

G

-

Y

N

E

G

N

R

V

Q

Q

P

P

I

L

-

L

-

F

-

R

A

S

S

Q

Q

N

D

N

G

G

A

S

L

L

Q

A

L

L

I

A

V

H

N

N

G

G

E

N

F

L

Y

V

D

N

F

A

E

T

R

Q

I

L

R

K

A

Q

E

Q

L

L

Q

E

A

N

R

Q

G

G

C

S

V

I

F

F

R

Q

T

T

Q

S

S

S

D

D

S

T

E

E

I

V

A

L

L

A

H

H

L

L

Y

I

R

K

E

R

F

S

G

G

-

H

-

F

-

T

-

L

-

K

-

D

-

Q

-

I

T

K

A

N

G

S

L

L

G

S

R

M

L

L

R

K

G

G

E

A

F

Y

A

A

L

F

V

L

L

I

Y

M

D

-

A

-

V

T

E

E

R

T

Q

E

L

M

V

I

V

V

M

A

R

L

D

D

R

P

F

N

G

G

I

L

K

R

P

P

L

L

F

S

V

I

A

G

E

M

H

M

D

G

G

D

A

A

L

Y

W

V

V

V

G

A

S

S

E

E

I

T

K

C

A

A

L

F

V

D

A

V

G

G

V

A

P

T

A

Y

F

L

W

R

D

E

L

V

D

E

Sites not aligning to the query:

1:11 modified: propeptide
12 active site, Nucleophile; C→F: Loss of enzyme activity and N-terminal processing.
247 binding [4Fe-4S] cluster
294 binding Mg(2+)
356 binding Mg(2+)
357 binding Mg(2+)
393 binding [4Fe-4S] cluster
394 F→V: Partial loss of activity.
442 D→S: Partial loss of activity.
448 binding [4Fe-4S] cluster; C→S: Loss of activity.
451 binding [4Fe-4S] cluster; C→S: Loss of activity.
452 F→C: Lethal.

1mbzA Beta-lactam synthetase with trapped intermediate (see paper)
25% identity, 40% coverage: 74:337/654 of query aligns to 67:306/496 of 1mbzA

query
sites
1mbzA

I

V

V

L

N
x
A

G
|
G

E

E

F

I

Y

Y

D

N

F

R

E

D

R

E

I

L

R

L

A

S

E

V

L

L

Q

P

A

A

R

G

G

P

C

A

V

-

F

-

R

-

T

P

Q

E

S

G

D

D

S

A

E

E

I

L

A

V

L

L

H

R

L

L

Y

L

R

E

E

R

F

Y

G

D

T

L

A

H

G

A

L

F

G

R

R

L

L

V

R

N

G

G

E

R

F

F

A

A

L

T

V

V

L

V

Y

R

D

T

A

G

V

-

E

-

R

D

Q

R

L

V

V

L

M

A

R

T

D

D

R

H

F

A

G

G

I

S

K

V

P

P

L

L

F

Y

V

T

A

C

E

V

H

A

D

P

G

G

A

E

L

V

W

R

V

A

G

S

S

T

E

E

I

A

K

K

A

A

L

L

V

A

A

A

A

H

G

G

V

F

P

P

A

L

F

A

W

-

D

-

L

-

D

D

A

A

Y

R

V

R

S

V

R

A

G

G

F

L

Y

-

L

-

G

-

N

-

R

-

T

-

L

-

F

-

Q

T

G

G

I

V

R

Y

S

Q

L

V

A

P

P

A

G

G

H

A

L

V

L

M

-

D

-

I

-

D

I

L

A

G

S

S

P

G

G

T

H

A

I

V

R

T

E

H

R

R

A

T

Y

W

W

-

D

-

L

-

D

-

F

-

P

-

A

-

A

T

A

P

A

G

L

L

D

S

S

R

A

R

A

I

I

L

D

P

E

E

A

G

E

E

A

A

V

V

E

A

G

A

L

V

R

R

A

A

E

A

I

L

L

E

E

K

A

A

V

V

G

A

L

Q

R

R

L

V

R

T

-

P

A

G

D

D

V

T

P

P

I

L

A

-

V

V

Y
x
V

L
|
L

S
|
S

G

G

G
|
G

I

I

D
|
D

S
|
S

S

S

A

G

M

V

L

A

G

A

C

C

A

A

T

H

R

R

L

A

R

A

G

G

T

E

P

-

L

L

D

D

A

T

F

V

T
x
S

L
x
M

S

G

F

T

T

D

D

T

D

S

G

N

D

E

Y

F

D

R

E

E

R

A

R

R

F

A

A

V

E

D

A

H

A

L

S

R

F

-

N

-

G

-

A

T

R

R

F

H

H

R

P

E

I

I

P

T

V

I

T

P

Q

T

D

T

D

E

L

L

A

L

D

A

D

Q

F

L

E

P

E

Y

A

A

L

V

W

W

H

A

N

S

E

E

active site: A69 (≠ N76), G70 (= G77)
binding arginine-n-methylcarbonyl phosphoric acid 5'-adenosine ester: V236 (≠ Y263), L237 (= L264), S238 (= S265), S243 (= S270), S261 (≠ T289), M262 (≠ L290)
binding magnesium ion: D242 (= D269)
binding pyrophosphate 2-: S238 (= S265), G240 (= G267), D242 (= D269), S243 (= S270)

Sites not aligning to the query:

active site: 311, 337, 371, 432
binding arginine-n-methylcarbonyl phosphoric acid 5'-adenosine ester: 315, 319, 336, 337, 338, 340, 341, 362, 371, 432, 434, 435
binding magnesium ion: 340
binding pyrophosphate 2-: 340, 412, 432, 433

1mb9A Beta-lactam synthetase complexed with atp (see paper)
24% identity, 40% coverage: 74:337/654 of query aligns to 68:305/485 of 1mb9A

query
sites
1mb9A

I

V

V

L

N
x
A

G
|
G

E

E

F

I

Y

Y

D

N

F

R

E

D

R

E

I

L

R

L

A

S

E

V

L

L

Q

P

A

A

R

G

G

P

C

A

V

-

F

-

R

-

T

P

Q

E

S

G

D

D

S

A

E

E

I

L

A

V

L

L

H

R

L

L

Y

L

R

E

E

R

F

Y

G

D

T

L

A

H

G

A

L

F

G

R

R

L

L

V

R

N

G

G

E

R

F

F

A

A

L

T

V

V

L

V

Y

R

D

T

A

G

V

-

E

-

R

D

Q

R

L

V

V

L

M

A

R

T

D

D

R

H

F

A

G

G

I

S

K

V

P

P

L

L

F

Y

V

T

A

C

E

V

H

A

D

P

G

G

A

E

L

V

W

R

V

A

G

S

S

T

E

E

I

A

K

K

A

A

L

L

V

A

A

A

-

F

-

P

-

L

-

A

-

D

-

A

-

R

-

V

A

A

G

G

V

L

P

T

A

G

F

V

W

Y

D

Q

L

V

D

P

A

A

Y

G

V

A

S

V

R

M

G

D

F

I

Y

D

L

L

G

G

N

S

R

G

T

T

L

A

F

V

Q

T

G

H

I

-

R

R

S

T

L

W

A

T

P

P

G

G

H

-

L

-

I

-

A

-

S

-

P

-

G

-

H

-

I

-

R

-

E

-

R

-

A

-

Y

-

W

-

D

-

L

-

D

-

F

-

P

-

A

-

L

L

D

S

S

R

A

R

A

I

I

L

D

P

E

E

A

G

E

E

A

A

V

V

E

A

G

A

L

V

R

R

A

A

E

A

I

L

L

E

E

K

A

A

V

V

G

A

L

Q

R

R

L

V

R

T

-

P

A

G

D

D

V

T

P

P

I

L

A

-

V

V

Y
x
V

L
|
L

S
|
S

G

G

G
|
G

I

I

D
|
D

S
|
S

S

S

A

G

M

V

L

A

G

A

C

C

A

A

T

H

R

R

L

A

R

A

G

G

T

E

P

-

L

L

D

D

A

T

F

V

T
x
S

L
x
M

S

G

F

T

T

D

D

T

D

S

G

N

D

E

Y

F

D

R

E

E

R

A

R

R

F

A

A

V

E

D

A

H

A

L

S

R

F

-

N

-

G

-

A

T

R

R

F

H

H

R

P

E

I

I

P

T

V

I

T

P

Q

T

D

T

D

E

L

L

A

L

D

A

D

Q

F

L

E

P

E

Y

A

A

L

V

W

W

H

A

N

S

E

E

active site: A70 (≠ N76), G71 (= G77)
binding adenosine monophosphate: V235 (≠ Y263), L236 (= L264), S242 (= S270), S260 (≠ T289), M261 (≠ L290)
binding adenosine-5'-triphosphate: V235 (≠ Y263), L236 (= L264), S237 (= S265), G239 (= G267), D241 (= D269), S242 (= S270), S260 (≠ T289), M261 (≠ L290)
binding magnesium ion: D241 (= D269)
binding pyrophosphate 2-: S237 (= S265), G239 (= G267), D241 (= D269), S242 (= S270)

Sites not aligning to the query:

active site: 310, 336, 370, 431
binding adenosine monophosphate: 314, 318, 335, 336
binding adenosine-5'-triphosphate: 318, 335, 339, 411, 431
binding magnesium ion: 339
binding pyrophosphate 2-: 339, 411, 431

1mc1A Beta-lactam synthetase with product (dgpc), amp and ppi (see paper)
24% identity, 40% coverage: 74:337/654 of query aligns to 63:301/491 of 1mc1A

query
sites
1mc1A

I

V

V

L

N
x
A

G
|
G

E

E

F

I

Y

Y

D

N

F

R

E

D

R

E

I

L

R

L

A

S

E

V

L

L

Q

P

A

A

R

G

G

P

C

A

V

-

F

-

R

-

T

P

Q

E

S

G

D

D

S

A

E

E

I

L

A

V

L

L

H

R

L

L

Y

L

R

E

E

R

F

Y

G

D

T

L

A

H

G

A

L

F

G

R

R

L

L

V

R

N

G

G

E

R

F

F

A

A

L

T

V

V

L

V

Y

R

D

T

A

G

V

-

E

-

R

D

Q

R

L

V

V

L

M

A

R

T

D

D

R

H

F

A

G

G

I

S

K

V

P

P

L

L

F

Y

V

T

A

C

E

V

H

A

D

P

G

G

A

E

L

V

W

R

V

A

G

S

S

T

E

E

I

A

K

K

A

A

L

L

V

A

A

A

A

-

G

-

V

-

P

-

A

-

F

-

W

-

D

-

L

-

D

-

A

-

Y

-

V

-

S

-

R

-

G

G

F

F

Y

P

L

L

G

A

N

D

R

A

T

R

L

R

F

V

Q

A

G

G

I

L

R

T

S

G

L

V

A

Y

P

-

G

-

H

-

L

-

I

-

A

-

S

-

P

-

G

-

H

Q

I

V

R

P

E

A

R

G

A

A

Y

V

W

M

D

D

L

I

D

D

F

L

P

G

A

S

A

G

A

T

A

A

-

V

-

T

-

H

-

R

-

T

-

W

-

T

-

P

-

G

L

L

D

S

S

R

A

R

A

I

I

L

D

P

E

E

A

G

E

E

A

A

V

V

E

A

G

A

L

V

R

R

A

A

E

A

I

L

L

E

E

K

A

A

V

V

G

A

L

Q

R

R

L

V

R

T

-

P

A

G

D

D

V

T

P

P

I

L

A

-

V

V

Y
x
V

L

L

S
|
S

G

G

G
|
G

I

I

D
|
D

S
|
S

S

S

A

G

M

V

L

A

G

A

C

C

A

A

T

H

R

R

L

A

R

A

G

G

T

E

P

-

L

L

D

D

A

T

F

V

T
x
S

L
x
M

S

G

F

T

T

D

D

T

D

S

G

N

D

E

Y

F

D

R

E

E

R

A

R

R

F

A

A

V

E

D

A

H

A

L

S

R

F

-

N

-

G

-

A

T

R

R

F

H

H

R

P

E

I

I

P

T

V

I

T

P

Q

T

D

T

D

E

L

L

A

L

D

A

D

Q

F

L

E

P

E

Y

A

A

L

V

W

W

H

A

N

S

E

E

active site: A65 (≠ N76), G66 (= G77)
binding adenosine monophosphate: V231 (≠ Y263), S233 (= S265), S238 (= S270), S256 (≠ T289), M257 (≠ L290)
binding magnesium ion: D237 (= D269)
binding pyrophosphate 2-: S233 (= S265), G235 (= G267), D237 (= D269), S238 (= S270)

Sites not aligning to the query:

active site: 306, 332, 366, 427
binding adenosine monophosphate: 331, 427, 430
binding magnesium ion: 335
binding deoxyguanidinoproclavaminic acid: 310, 332, 333, 336, 357, 366, 427
binding pyrophosphate 2-: 335, 407, 427, 428

Query Sequence

>WP_012631017.1 NCBI__GCF_000022085.1:WP_012631017.1
MCGFLAVFHGGPRPLAPSTILAGLSAIRHRGPDERDIWLDPEAGVALGHTRLSIIGLSNG
RQPIASQDGALQLIVNGEFYDFERIRAELQARGCVFRTQSDSEIALHLYREFGTAGLGRL
RGEFALVLYDAVERQLVVMRDRFGIKPLFVAEHDGALWVGSEIKALVAAGVPAFWDLDAY
VSRGFYLGNRTLFQGIRSLAPGHLLIASPGHIRERAYWDLDFPAAAALDSAAIDEAEAVE
GLRAEILEAVGLRLRADVPIAVYLSGGIDSSAMLGCATRLRGTPLDAFTLSFTDDGDYDE
RRFAAEAASFNGARFHPIPVTQDDLADDFEEALWHNEVPFFNAHGVAKYRLSRVVAQARF
KVVITGEGADEIFAGYPHFRRDMLLYNAERQDPGLVADLRARLARSEGGYGGTTLPADAA
WMTDRLGHGVSWVGNQSAWFDALRALYDGPTRRRSAGIGAYRRFYNGLDHARMAGRDPLH
RSMYLWAKSFLPNFVLTTLGDRMEMANSIEGRVPLLDHHVAEYAARLPVWLKIRGATEKH
VFREAMRPFLPEALYRRKKHYFRAPPAMSAQRNRLAQLVADTLASRHLEDLPFFDARAVR
KAHAEAAALPPERQALLDPMFTEITSLCLMQRRFGLATHPATLSPEASVREAAA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory