SitesBLAST
Comparing WP_012645265.1 NCBI__GCF_000022265.1:WP_012645265.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
42% identity, 99% coverage: 1:258/260 of query aligns to 1:257/259 of 5zaiC
- active site: A65 (= A65), F70 (≠ M70), S82 (= S82), R86 (≠ Q86), G110 (= G110), E113 (= E113), P132 (= P133), E133 (= E134), I138 (= I139), P140 (= P141), G141 (= G142), A226 (= A227), F236 (≠ S237)
- binding coenzyme a: K24 (≠ T24), L25 (vs. gap), A63 (= A63), G64 (= G64), A65 (= A65), D66 (= D66), I67 (= I67), P132 (= P133), R166 (≠ T167), F248 (= F249), K251 (= K252)
3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
40% identity, 99% coverage: 1:257/260 of query aligns to 1:254/255 of 3q0jC
- active site: A65 (= A65), M70 (= M70), T80 (≠ S82), F84 (≠ Q86), G108 (= G110), E111 (= E113), P130 (= P133), E131 (= E134), V136 (≠ I139), P138 (= P141), G139 (= G142), L224 (≠ A227), F234 (≠ S237)
- binding acetoacetyl-coenzyme a: Q23 (≠ P23), A24 (≠ T24), L25 (vs. gap), A27 (≠ P26), A63 (= A63), G64 (= G64), A65 (= A65), D66 (= D66), I67 (= I67), K68 (= K68), M70 (= M70), F84 (≠ Q86), G107 (= G109), G108 (= G110), E111 (= E113), P130 (= P133), E131 (= E134), P138 (= P141), G139 (= G142), M140 (≠ A143)
3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
40% identity, 99% coverage: 1:257/260 of query aligns to 1:254/255 of 3q0gC
- active site: A65 (= A65), M70 (= M70), T80 (≠ S82), F84 (≠ Q86), G108 (= G110), E111 (= E113), P130 (= P133), E131 (= E134), V136 (≠ I139), P138 (= P141), G139 (= G142), L224 (≠ A227), F234 (≠ S237)
- binding coenzyme a: L25 (vs. gap), A63 (= A63), I67 (= I67), K68 (= K68), Y104 (≠ H106), P130 (= P133), E131 (= E134), L134 (= L137)
3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
40% identity, 99% coverage: 2:259/260 of query aligns to 1:255/256 of 3h81A
- active site: A64 (= A65), M69 (= M70), T79 (≠ S82), F83 (≠ Q86), G107 (= G110), E110 (= E113), P129 (= P133), E130 (= E134), V135 (≠ I139), P137 (= P141), G138 (= G142), L223 (≠ A227), F233 (≠ S237)
- binding calcium ion: F233 (≠ S237), Q238 (≠ A242)
3q0gD Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
40% identity, 98% coverage: 2:257/260 of query aligns to 1:249/250 of 3q0gD
- active site: A64 (= A65), M69 (= M70), T75 (≠ E77), F79 (= F81), G103 (= G110), E106 (= E113), P125 (= P133), E126 (= E134), V131 (≠ I139), P133 (= P141), G134 (= G142), L219 (≠ A227), F229 (≠ S237)
- binding Butyryl Coenzyme A: F225 (≠ S233), F241 (= F249)
P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
40% identity, 97% coverage: 10:260/260 of query aligns to 43:290/290 of P14604
- E144 (= E113) mutation to D: Reduces activity 50-fold.; mutation to Q: Reduces activity 3300-fold.
- E164 (= E134) mutation to D: Reduces activity 1250-fold.; mutation to Q: Reduces activity 330000-fold.
Sites not aligning to the query:
- 1:29 modified: transit peptide, Mitochondrion
1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
40% identity, 97% coverage: 10:260/260 of query aligns to 13:260/260 of 1dubA
- active site: A68 (= A65), M73 (= M70), S83 (= S82), L87 (≠ Q86), G111 (= G110), E114 (= E113), P133 (= P133), E134 (= E134), T139 (≠ I139), P141 (= P141), G142 (= G142), K227 (≠ A227), F237 (≠ S237)
- binding acetoacetyl-coenzyme a: K26 (≠ P23), A27 (≠ T24), L28 (vs. gap), A30 (≠ P26), A66 (= A63), A68 (= A65), D69 (= D66), I70 (= I67), Y107 (≠ H106), G110 (= G109), G111 (= G110), E114 (= E113), P133 (= P133), E134 (= E134), L137 (= L137), G142 (= G142), F233 (≠ S233), F249 (= F249)
1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
40% identity, 97% coverage: 10:260/260 of query aligns to 11:258/258 of 1ey3A
- active site: A66 (= A65), M71 (= M70), S81 (= S82), L85 (≠ Q86), G109 (= G110), E112 (= E113), P131 (= P133), E132 (= E134), T137 (≠ I139), P139 (= P141), G140 (= G142), K225 (≠ A227), F235 (≠ S237)
- binding 4-(n,n-dimethylamino)cinnamoyl-coa: K24 (≠ P23), L26 (vs. gap), A28 (≠ P26), A64 (= A63), G65 (= G64), A66 (= A65), D67 (= D66), I68 (= I67), L85 (≠ Q86), W88 (≠ N89), G109 (= G110), P131 (= P133), L135 (= L137), G140 (= G142)
1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
40% identity, 97% coverage: 10:260/260 of query aligns to 13:258/258 of 1mj3A
- active site: A68 (= A65), M73 (= M70), S83 (= S82), L85 (= L84), G109 (= G110), E112 (= E113), P131 (= P133), E132 (= E134), T137 (≠ I139), P139 (= P141), G140 (= G142), K225 (≠ A227), F235 (≠ S237)
- binding hexanoyl-coenzyme a: K26 (≠ P23), A27 (≠ T24), L28 (vs. gap), A30 (≠ P26), A66 (= A63), G67 (= G64), A68 (= A65), D69 (= D66), I70 (= I67), G109 (= G110), P131 (= P133), E132 (= E134), L135 (= L137), G140 (= G142)
2dubA Enoyl-coa hydratase complexed with octanoyl-coa (see paper)
40% identity, 97% coverage: 10:260/260 of query aligns to 12:254/254 of 2dubA
- active site: A67 (= A65), M72 (= M70), S82 (= S82), G105 (= G110), E108 (= E113), P127 (= P133), E128 (= E134), T133 (≠ I139), P135 (= P141), G136 (= G142), K221 (≠ A227), F231 (≠ S237)
- binding octanoyl-coenzyme a: K25 (≠ P23), A26 (≠ T24), L27 (vs. gap), A29 (≠ P26), A65 (= A63), A67 (= A65), D68 (= D66), I69 (= I67), K70 (= K68), G105 (= G110), E108 (= E113), P127 (= P133), E128 (= E134), G136 (= G142), A137 (= A143)
2hw5C The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1
38% identity, 100% coverage: 2:260/260 of query aligns to 2:260/260 of 2hw5C
- active site: A68 (= A65), M73 (= M70), S83 (= S82), L87 (≠ Q86), G111 (= G110), E114 (= E113), P133 (= P133), E134 (= E134), T139 (≠ I139), P141 (= P141), G142 (= G142), K227 (≠ A227), F237 (≠ S237)
- binding crotonyl coenzyme a: K26 (≠ P23), A27 (≠ T24), L28 (vs. gap), A30 (≠ P26), K62 (= K59), I70 (= I67), F109 (≠ L108)
5jbxB Crystal structure of liuc in complex with coenzyme a and malonic acid (see paper)
41% identity, 90% coverage: 25:259/260 of query aligns to 27:260/261 of 5jbxB
- active site: A67 (= A65), R72 (≠ M70), L84 (≠ S82), R88 (≠ Q86), G112 (= G110), E115 (= E113), T134 (≠ P133), E135 (= E134), I140 (= I139), P142 (= P141), G143 (= G142), A228 (= A227), L238 (≠ S237)
- binding coenzyme a: A28 (≠ P26), A65 (= A63), D68 (= D66), L69 (≠ I67), K70 (= K68), L110 (= L108), G111 (= G109), T134 (≠ P133), E135 (= E134), L138 (= L137), R168 (≠ T167)
Sites not aligning to the query:
6slaAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
34% identity, 98% coverage: 5:258/260 of query aligns to 1:243/245 of 6slaAAA
- active site: Q61 (≠ A65), L68 (≠ A78), N72 (≠ S82), A96 (≠ G110), S99 (≠ E113), A118 (≠ P133), F119 (≠ E134), L124 (≠ I139), P126 (= P141), N127 (≠ G142), A212 (= A227), G222 (≠ S237)
- binding ~{S}-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] 2-(2,5-dihydrooxepin-7-yl)ethanethioate: L21 (≠ T24), A59 (= A63), Q61 (≠ A65), D62 (= D66), L63 (≠ I67), L68 (≠ A78), Y71 (≠ F81), A94 (≠ L108), G95 (= G109), A96 (≠ G110), F119 (≠ E134), I122 (≠ L137), L124 (≠ I139), N127 (≠ G142), F234 (= F249), K237 (= K252)
6slbAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
33% identity, 98% coverage: 5:258/260 of query aligns to 4:255/257 of 6slbAAA
- active site: Q64 (≠ A65), F69 (≠ M70), L80 (≠ A78), N84 (≠ S82), A108 (≠ G110), S111 (≠ E113), A130 (≠ P133), F131 (≠ E134), L136 (≠ I139), P138 (= P141), D139 (≠ G142), A224 (= A227), G234 (≠ S237)
- binding (~{E})-6-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-6-oxidanylidene-hex-3-enoic acid: R58 (≠ K59), A62 (= A63), Q64 (≠ A65), D65 (= D66), L66 (≠ I67), Y76 (vs. gap), A108 (≠ G110), F131 (≠ E134), D139 (≠ G142)
P40939 Trifunctional enzyme subunit alpha, mitochondrial; 78 kDa gastrin-binding protein; Monolysocardiolipin acyltransferase; TP-alpha; EC 2.3.1.-; EC 4.2.1.17; EC 1.1.1.211 from Homo sapiens (Human) (see 5 papers)
40% identity, 68% coverage: 8:185/260 of query aligns to 45:224/763 of P40939
Sites not aligning to the query:
- 282 V → D: in MTPD1; mild phenotype with slowly progressive myopathy and sensorimotor polyneuropathy; dbSNP:rs137852773
- 305 I → N: in MTPD1; mild phenotype with slowly progressive myopathy and sensorimotor polyneuropathy; dbSNP:rs137852774
- 342 L → P: in LCHAD deficiency; dbSNP:rs137852772
- 510 active site, For hydroxyacyl-coenzyme A dehydrogenase activity; E → Q: in AFLP and LCHAD deficiency; loss of long-chain-3-hydroxyacyl-CoA dehydrogenase activity; dbSNP:rs137852769
6tnmA E. Coli aerobic trifunctional enzyme subunit-alpha (see paper)
40% identity, 72% coverage: 1:188/260 of query aligns to 1:193/719 of 6tnmA
Sites not aligning to the query:
6eqoA Tri-functional propionyl-coa synthase of erythrobacter sp. Nap1 with bound NADP+ and phosphomethylphosphonic acid adenylate ester (see paper)
35% identity, 93% coverage: 3:244/260 of query aligns to 851:1086/1804 of 6eqoA