SitesBLAST
Comparing WP_012674125.1 NCBI__GCF_000021545.1:WP_012674125.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P80874 Aldo-keto reductase YhdN; AKR11B; General stress protein 69; GSP69; EC 1.1.1.- from Bacillus subtilis (strain 168) (see paper)
61% identity, 99% coverage: 2:331/332 of query aligns to 1:328/331 of P80874
- TW 20:21 (= TW 21:22) binding NADP(+)
- D52 (= D53) binding NADP(+)
- Q175 (= Q178) binding NADP(+)
- YGSLCR 203:208 (≠ YGAICR 206:211) binding NADP(+)
- K214 (= K217) binding NADP(+)
- R227 (= R230) binding NADP(+)
- GAR 280:282 (≠ GVR 283:285) binding NADP(+)
- Q286 (≠ H289) binding NADP(+)
1pz1A Structure of NADPH-dependent family 11 aldo-keto reductase akr11b(holo) (see paper)
60% identity, 99% coverage: 2:331/332 of query aligns to 1:328/333 of 1pz1A
- active site: D52 (= D53), Y57 (= Y58), K90 (= K93), Q93 (≠ K96), H125 (= H128)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G19 (= G20), W21 (= W22), Q175 (= Q178), Y203 (= Y206), G204 (= G207), L206 (≠ I209), R208 (= R211), K214 (= K217), G280 (= G283), R282 (= R285), Q286 (≠ H289)
1pz0A Structure of NADPH-dependent family 11 aldo-keto reductase akr11a(holo) (see paper)
35% identity, 93% coverage: 6:313/332 of query aligns to 4:308/311 of 1pz0A
- active site: D52 (= D53), Y57 (= Y58), N91 (≠ H94), H124 (= H128)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: H124 (= H128), Q174 (= Q178), Y202 (= Y206), F203 (≠ G207), P204 (≠ A208), L205 (≠ I209), S207 (≠ R211), G208 (= G212), A211 (≠ S215), K213 (= K217)
P46336 Aldo-keto reductase IolS; AKR11A; Vegetative protein 147; VEG147; EC 1.1.1.- from Bacillus subtilis (strain 168) (see paper)
35% identity, 92% coverage: 6:312/332 of query aligns to 5:308/310 of P46336
P77256 NADH-specific methylglyoxal reductase; AKR11B2; EC 1.1.1.- from Escherichia coli (strain K12) (see paper)
36% identity, 92% coverage: 6:309/332 of query aligns to 3:317/326 of P77256
- D232 (≠ N221) mutation D->A,E: Converts the protein into an enzyme with dual specificity, i.e. that is able to use both NADPH and NADH as cosubstrates.
6kiyA Crystal structure of a thermostable aldo-keto reductase tm1743 in complex with inhibitor epalrestat (see paper)
31% identity, 93% coverage: 4:312/332 of query aligns to 4:274/275 of 6kiyA
- binding {5-[(2E)-2-methyl-3-phenylprop-2-en-1-ylidene]-4-oxo-2-thioxo-1,3-thiazolidin-3-yl}acetic acid: W22 (= W22), Y59 (= Y58), W87 (= W98), H118 (= H128), R204 (= R211)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G20 (= G20), T21 (= T21), W22 (= W22), Y59 (= Y58), H118 (= H128), N149 (= N159), Q170 (= Q178), Y199 (= Y206), S200 (≠ G207), P201 (≠ A208), L202 (≠ I209), R204 (= R211), T205 (≠ G212), Y227 (≠ I264), I243 (≠ L281), P244 (≠ W282), K245 (≠ G283), G247 (≠ R285), R248 (≠ K286), H251 (= H289), E254 (≠ D292), N255 (≠ I293)
6kikA Crystal structure of a thermostable aldo-keto reductase tm1743 in complex with inhibitor tolrestat (see paper)
31% identity, 93% coverage: 4:312/332 of query aligns to 4:274/275 of 6kikA
5danA Crystal structure of a novel aldo keto reductase tm1743 from thermotoga maritima in complex with NADP+
31% identity, 90% coverage: 13:312/332 of query aligns to 12:273/274 of 5danA
- active site: D53 (= D53), Y58 (= Y58), K84 (= K96), H117 (= H128)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G19 (= G20), T20 (= T21), W21 (= W22), D53 (= D53), Y58 (= Y58), H117 (= H128), Q169 (= Q178), Y198 (= Y206), S199 (≠ G207), P200 (≠ A208), L201 (≠ I209), R203 (= R211), Y226 (≠ I264), I242 (≠ L281), P243 (≠ W282), K244 (≠ G283), G246 (≠ R285), R247 (≠ K286), H250 (= H289), E253 (≠ D292), N254 (≠ I293)
Q3L181 Perakine reductase; EC 1.1.1.317 from Rauvolfia serpentina (Serpentine wood) (Ophioxylon serpentinum) (see paper)
31% identity, 92% coverage: 9:313/332 of query aligns to 7:309/337 of Q3L181
- D52 (= D53) mutation to A: 99% loss of activity.
- Y57 (= Y58) mutation to A: 99% loss of activity.
- K84 (= K85) mutation to A: Total loss of activity.
- H126 (= H128) mutation to A: 98% loss of activity.
4wghA Crystal structure of aldo/keto reductase from klebsiella pneumoniae in complex with NADP and acetate at 1.8 a resolution
36% identity, 67% coverage: 9:229/332 of query aligns to 8:211/283 of 4wghA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G19 (= G20), T20 (= T21), W21 (= W22), D49 (= D53), Y54 (= Y58), N142 (= N159), Q164 (= Q178), Y192 (= Y206), C193 (= C210), P194 (≠ R211), L195 (≠ G212), Q197 (≠ L214), A198 (≠ S215), R200 (≠ K217)
Sites not aligning to the query:
6ciaA Crystal structure of aldo-keto reductase from klebsiella pneumoniae in complex with NADPH.
36% identity, 67% coverage: 9:229/332 of query aligns to 9:212/284 of 6ciaA
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G20 (= G20), T21 (= T21), W22 (= W22), D50 (= D53), Y55 (= Y58), H113 (= H128), N143 (= N159), Q165 (= Q178), Y193 (= Y206), C194 (= C210), P195 (≠ R211), L196 (≠ G212), Q198 (≠ L214), A199 (≠ S215)
Sites not aligning to the query:
1ynqB Aldo-keto reductase akr11c1 from bacillus halodurans (holo form) (see paper)
37% identity, 62% coverage: 10:215/332 of query aligns to 10:206/298 of 1ynqB
- active site: D47 (= D53), Y52 (= Y58), K86 (≠ H94), H120 (= H128)
- binding beta-D-fructofuranose: N44 (= N50), D71 (≠ K79)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G20 (= G20), C21 (≠ T21), M22 (≠ W22), S23 (≠ A23), D47 (= D53), Y52 (= Y58), K77 (= K85), S150 (= S158), M170 (≠ Q178), R197 (≠ Y206), G198 (= G207), P199 (≠ A208), V200 (≠ I209), R202 (= R211), S206 (= S215)
Sites not aligning to the query:
1ynpB Aldo-keto reductase akr11c1 from bacillus halodurans (apo form) (see paper)
37% identity, 62% coverage: 10:215/332 of query aligns to 10:206/298 of 1ynpB
Sites not aligning to the query:
8hw0A The structure of akr6d1
28% identity, 97% coverage: 2:324/332 of query aligns to 1:327/329 of 8hw0A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G19 (= G20), W21 (= W22), Q27 (≠ M28), D49 (= D53), Y54 (= Y58), R123 (≠ W129), S152 (= S158), Q178 (= Q178), W207 (≠ Y206), S208 (≠ G207), P209 (≠ A208), L210 (≠ I209), S212 (≠ R211), K218 (= K217), S227 (≠ T222), R228 (≠ K223), I285 (≠ L281), G287 (= G283), S289 (≠ R285), Q293 (≠ H289), D296 (= D292), N297 (≠ I293)
8jwmB Crystal structure of akrtyl-NADP-tylosin complex (see paper)
31% identity, 94% coverage: 2:313/332 of query aligns to 1:319/331 of 8jwmB
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G19 (= G20), M21 (≠ W22), D48 (= D53), Y53 (= Y58), N161 (= N159), Q186 (= Q178), W214 (≠ Y206), S215 (≠ G207), P216 (≠ A208), L217 (≠ I209), G219 (≠ R211), G220 (= G212), R235 (≠ L229), R236 (= R230), A237 (≠ K231), I287 (≠ L281), G289 (= G283), R291 (= R285), Q295 (≠ H289), S298 (≠ D292)
- binding tylosin: Y53 (= Y58), W55 (≠ F60), H130 (= H128), C192 (≠ F184), E193 (= E185), R195 (≠ Q187), G240 (≠ P234), R241 (≠ K235), Q250 (≠ R240), E253 (= E243), Q254 (≠ Y244), R257 (≠ A247), E317 (≠ K311), I318 (= I312)
Sites not aligning to the query:
3v0sA Crystal structure of perakine reductase, founder member of a novel akr subfamily with unique conformational changes during NADPH binding (see paper)
30% identity, 92% coverage: 9:313/332 of query aligns to 7:277/287 of 3v0sA
- active site: D45 (= D53), Y50 (= Y58), G87 (≠ K96), H119 (= H128)
- binding 2'-monophosphoadenosine-5'-diphosphate: S198 (≠ G207), P199 (≠ A208), I200 (= I209), G201 (≠ C210), L204 (= L213), P246 (≠ W282), G247 (= G283), T248 (≠ V284), T249 (≠ R285), N253 (≠ H289), N256 (≠ D292), N257 (≠ I293)
8jwkD The second purified state crystal structure of akrtyl (see paper)
30% identity, 94% coverage: 2:313/332 of query aligns to 1:302/314 of 8jwkD
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G19 (= G20), M21 (≠ W22), D48 (= D53), Y53 (= Y58), H130 (= H128), Q186 (= Q178), W214 (≠ Y206), S215 (≠ G207), P216 (≠ A208), L217 (≠ I209), I270 (≠ L281), G272 (= G283), R274 (= R285), Q278 (≠ H289), S281 (≠ D292)
8hnqA The structure of a alcohol dehydrogenase akr13b2 with NADP
29% identity, 91% coverage: 9:310/332 of query aligns to 20:286/286 of 8hnqA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G31 (= G20), M33 (≠ W22), R34 (≠ A23), Y71 (= Y58), R138 (≠ W129), Q187 (= Q178), W214 (≠ Y206), A215 (≠ G207), P216 (≠ A208), L217 (≠ I209), S219 (≠ R211), G220 (= G212), I257 (≠ L281), P258 (≠ W282), G259 (= G283), T260 (≠ V284), K262 (= K286), H265 (= H289), E268 (≠ D292), N269 (≠ I293)
- binding 1,4,7,10,13,16-hexaoxacyclooctadecane: L98 (= L88), W106 (≠ K96), R138 (≠ W129)
8jwoL Crystal structure of akrtyl-tylosin complex (see paper)
30% identity, 94% coverage: 2:313/332 of query aligns to 1:299/311 of 8jwoL
Sites not aligning to the query:
1ynpA Aldo-keto reductase akr11c1 from bacillus halodurans (apo form) (see paper)
38% identity, 57% coverage: 26:215/332 of query aligns to 20:191/283 of 1ynpA
Sites not aligning to the query:
Query Sequence
>WP_012674125.1 NCBI__GCF_000021545.1:WP_012674125.1
MVEYLKIPGTEIEVSRIAIGTWAIGGWMWGGTDEQKAIEAILNGLDKGLNLIDTAPVYGF
GLSEEIVGKALKEYGSRDKVVIATKVGLEWEDKHSKVWRNSTKERIFKEVEDSLKRLQTD
YIDIYQVHWPDTKTPFEETAEAMYKLYKEGKIRAIGVSNYSPEQMEEFRKVAPIYTNQPP
YNLFERQIEKDVILYCEKNNIALLFYGAICRGLLSGKINKNTKFEGDDLRKVDPKFQEPR
FSEYLSAVEELDKYAQERFGKRVIHLAVRWMLDKSPLGVALWGVRKKEHLNDIPEVFGWH
LSEEDFKNMDKIINENVKHPVGPEFMSPPERP
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory