SitesBLAST
Comparing WP_012674308.1 NCBI__GCF_000021545.1:WP_012674308.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
1i2lA Deoxychorismate lyase from escherichia coli with inhibitor
25% identity, 99% coverage: 4:249/249 of query aligns to 2:253/269 of 1i2lA
- active site: F26 (= F27), T28 (= T29), I139 (= I135), K140 (= K136), E173 (= E169), V197 (≠ L193)
- binding d-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-n,o-cycloserylamide: R45 (= R46), K140 (= K136), E173 (= E169), C175 (≠ S171), A176 (≠ S172), A177 (= A173), N178 (= N174), V197 (≠ L193), G199 (= G195), I200 (= I196), M201 (≠ S197), N236 (= N232), A237 (= A233)
1i2kA Aminodeoxychorismate lyase from escherichia coli
25% identity, 99% coverage: 4:249/249 of query aligns to 2:253/269 of 1i2kA
- active site: F26 (= F27), T28 (= T29), I139 (= I135), K140 (= K136), E173 (= E169), V197 (≠ L193)
- binding pyridoxal-5'-phosphate: R45 (= R46), K140 (= K136), E173 (= E169), A176 (≠ S172), A177 (= A173), V197 (≠ L193), G199 (= G195), I200 (= I196), M201 (≠ S197), N236 (= N232), A237 (= A233)
5mr0D Thermophilic archaeal branched-chain amino acid transaminases from geoglobus acetivorans and archaeoglobus fulgidus: biochemical and structural characterisation (see paper)
30% identity, 90% coverage: 20:244/249 of query aligns to 25:257/290 of 5mr0D
- active site: F32 (= F27), G34 (≠ T29), K150 (= K136), E183 (= E169), L206 (= L193)
- binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: R51 (= R46), G100 (≠ P95), L101 (= L96), K150 (= K136), Y154 (= Y140), E183 (= E169), G186 (≠ S172), D187 (≠ A173), L206 (= L193), I209 (= I196), T210 (≠ S197), G245 (≠ N232), T246 (≠ A233)
5e25A Crystal structure of branched-chain aminotransferase from thermophilic archaea geoglobus acetivorans complexed with alpha-ketoglutarate (see paper)
32% identity, 90% coverage: 20:244/249 of query aligns to 26:258/290 of 5e25A
- active site: F33 (= F27), G35 (≠ T29), K151 (= K136), E184 (= E169), L207 (= L193)
- binding 2-oxoglutaric acid: Y88 (= Y81), K151 (= K136), T247 (≠ A233), A248 (≠ L234)
- binding pyridoxal-5'-phosphate: R52 (= R46), K151 (= K136), Y155 (= Y140), E184 (= E169), G187 (≠ S172), D188 (≠ A173), L207 (= L193), G209 (= G195), I210 (= I196), T211 (≠ S197), G246 (≠ N232), T247 (≠ A233)
6thqB Crystal structure of branched-chain aminotransferase from thermophilic archaea thermoproteus uzoniensis with norvaline
26% identity, 99% coverage: 3:249/249 of query aligns to 8:270/301 of 6thqB
- active site: F37 (= F27), K156 (≠ I135), E190 (= E169), L214 (= L193)
- binding pyridoxal-5'-phosphate: R60 (= R46), K156 (≠ I135), Y161 (= Y140), E190 (= E169), N195 (= N174), L214 (= L193), G216 (= G195), I217 (= I196), T218 (≠ S197), T254 (≠ A233)
- binding 2-[o-phosphonopyridoxyl]-amino-pentanoic acid: R60 (= R46), Y97 (= Y81), K156 (≠ I135), Y161 (= Y140), E190 (= E169), G193 (≠ S172), E194 (≠ A173), N195 (= N174), G216 (= G195), I217 (= I196), T218 (≠ S197), G253 (≠ N232), T254 (≠ A233), A255 (≠ L234)
8yrvA Crystal structure of d-amino acid transaminase from haliscomenobacter hydrossis complexed with 3-aminooxypropionic acid
25% identity, 95% coverage: 12:248/249 of query aligns to 24:252/279 of 8yrvA
- binding 3-[(~{E})-[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]oxypropanoic acid: Y37 (≠ T29), R54 (= R46), R135 (≠ V126), K141 (= K136), Y145 (= Y140), E174 (= E169), A176 (≠ S171), R177 (≠ S172), S178 (≠ A173), N179 (= N174), L197 (= L193), G199 (= G195), I200 (= I196), T201 (≠ S197), T237 (≠ A233), I238 (≠ L234)
8ahuA Crystal structure of d-amino acid aminotrensferase from haliscomenobacter hydrossis complexed with d-cycloserine (see paper)
24% identity, 95% coverage: 12:248/249 of query aligns to 24:256/283 of 8ahuA
- binding [5-hydroxy-6-methyl-4-({[(4E)-3-oxo-1,2-oxazolidin-4-ylidene]amino}methyl)pyridin-3-yl]methyl dihydrogen phosphate: F35 (= F27), R54 (= R46), R139 (≠ V126), K145 (= K136), Y149 (= Y140), E178 (= E169), A180 (≠ S171), R181 (≠ S172), S182 (≠ A173), L201 (= L193), G203 (= G195), I204 (= I196), T205 (≠ S197), S240 (≠ N232), T241 (≠ A233)
7p7xA Crystal structure of d-amino acid transaminase from haliscomenobacter hydrossis (holo form). (see paper)
24% identity, 95% coverage: 12:248/249 of query aligns to 23:255/282 of 7p7xA
- binding pyridoxal-5'-phosphate: R53 (= R46), R138 (≠ V126), K144 (= K136), Y148 (= Y140), E177 (= E169), A179 (≠ S171), R180 (≠ S172), S181 (≠ A173), L200 (= L193), G202 (= G195), I203 (= I196), T204 (≠ S197), T240 (≠ A233)
- binding phosphate ion: L47 (≠ I40), D48 (= D41), R73 (≠ L65)
Sites not aligning to the query:
8raiA Crystal structure of d-amino acid transaminase from haliscomenobacter hydrossis point mutant r90i complexed with phenylhydrazine (see paper)
24% identity, 95% coverage: 12:248/249 of query aligns to 23:255/282 of 8raiA
- binding [6-methyl-5-oxidanyl-4-[(2-phenylhydrazinyl)methyl]pyridin-3-yl]methyl dihydrogen phosphate: F34 (= F27), R53 (= R46), R138 (≠ V126), K144 (= K136), Y148 (= Y140), E177 (= E169), A179 (≠ S171), R180 (≠ S172), S181 (≠ A173), N182 (= N174), L200 (= L193), G202 (= G195), I203 (= I196), T204 (≠ S197), T240 (≠ A233)
7dbeB Structure of a novel transaminase
27% identity, 81% coverage: 43:244/249 of query aligns to 85:296/332 of 7dbeB
- binding pyridoxal-5'-phosphate: R88 (= R46), K190 (= K134), E223 (= E169), G226 (≠ S172), F227 (≠ A173), N228 (= N174), L245 (= L193), G247 (= G195), I248 (= I196), T249 (≠ S197), T285 (≠ A233)
8pnyA Crystal structure of d-amino acid aminotransferase from blastococcus saxobsidens complexed with phenylhydrazine and in its apo form (see paper)
20% identity, 93% coverage: 13:244/249 of query aligns to 26:260/278 of 8pnyA
- binding [6-methyl-5-oxidanyl-4-[(2-phenylhydrazinyl)methyl]pyridin-3-yl]methyl dihydrogen phosphate: F36 (= F27), S38 (≠ T29), R55 (= R46), K152 (= K136), Y156 (= Y140), E185 (= E169), G186 (≠ T170), P187 (≠ S171), T188 (≠ S172), S189 (≠ A173), T190 (≠ N174), L209 (= L193), T212 (≠ I196), T213 (≠ S197), S248 (≠ N232), G249 (≠ A233)
8pnxA Crystal structure of d-amino acid aminotransferase from blastococcus saxobsidens in pmp form
20% identity, 93% coverage: 13:244/249 of query aligns to 26:260/278 of 8pnxA
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R55 (= R46), K152 (= K136), Y156 (= Y140), E185 (= E169), T188 (≠ S172), S189 (≠ A173), T190 (≠ N174), L209 (= L193), T212 (≠ I196), T213 (≠ S197), S248 (≠ N232), G249 (≠ A233)
8pnwA Crystal structure of d-amino acid aminotransferase from blastococcus saxobsidens in holo form with plp (see paper)
20% identity, 93% coverage: 13:244/249 of query aligns to 26:260/278 of 8pnwA
- binding pyridoxal-5'-phosphate: R55 (= R46), K152 (= K136), Y156 (= Y140), E185 (= E169), P187 (≠ S171), T188 (≠ S172), S189 (≠ A173), L209 (= L193), T212 (≠ I196), T213 (≠ S197), G249 (≠ A233)
1iyeA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
25% identity, 92% coverage: 16:244/249 of query aligns to 22:265/304 of 1iyeA
- active site: F33 (= F27), G35 (≠ T29), K156 (≠ I135), A157 (≠ K136), E190 (= E169), L214 (= L193)
- binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: R56 (= R46), Y92 (= Y81), Y126 (vs. gap), K156 (≠ I135), Y161 (= Y140), E190 (= E169), G193 (≠ S172), E194 (≠ A173), N195 (= N174), L214 (= L193), G216 (= G195), I217 (= I196), T218 (≠ S197), G253 (≠ N232), T254 (≠ A233), A255 (≠ L234)
1iydA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
25% identity, 92% coverage: 16:244/249 of query aligns to 22:265/304 of 1iydA
- active site: F33 (= F27), G35 (≠ T29), K156 (≠ I135), A157 (≠ K136), E190 (= E169), L214 (= L193)
- binding glutaric acid: Y92 (= Y81), Y126 (vs. gap), A255 (≠ L234)
- binding pyridoxal-5'-phosphate: R56 (= R46), K156 (≠ I135), Y161 (= Y140), E190 (= E169), G193 (≠ S172), E194 (≠ A173), L214 (= L193), G216 (= G195), I217 (= I196), T218 (≠ S197), T254 (≠ A233)
1i1mA Crystal structure of escherichia coli branched-chain amino acid aminotransferase. (see paper)
25% identity, 92% coverage: 16:244/249 of query aligns to 22:265/304 of 1i1mA
- active site: K156 (≠ I135)
- binding 4-methyl valeric acid: Y92 (= Y81), K156 (≠ I135), T254 (≠ A233), A255 (≠ L234)
- binding pyridoxal-5'-phosphate: R56 (= R46), K156 (≠ I135), Y161 (= Y140), E190 (= E169), G193 (≠ S172), E194 (≠ A173), L214 (= L193), G216 (= G195), I217 (= I196), T218 (≠ S197), G253 (≠ N232), T254 (≠ A233)
1i1lA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase. (see paper)
25% identity, 92% coverage: 16:244/249 of query aligns to 22:265/304 of 1i1lA
- active site: K156 (≠ I135)
- binding 2-methylleucine: Y92 (= Y81), K156 (≠ I135), T254 (≠ A233), A255 (≠ L234)
- binding pyridoxal-5'-phosphate: R56 (= R46), K156 (≠ I135), Y161 (= Y140), E190 (= E169), G193 (≠ S172), G216 (= G195), I217 (= I196), T218 (≠ S197), T254 (≠ A233)
3cswC Crystal structure of a putative branched-chain amino acid aminotransferase (tm0831) from thermotoga maritima at 2.15 a resolution
27% identity, 95% coverage: 13:248/249 of query aligns to 20:245/275 of 3cswC
- active site: Y33 (≠ F27), K135 (= K134), E166 (= E169), L190 (= L193)
- binding pyridoxal-5'-phosphate: R52 (= R46), K135 (= K134), E166 (= E169), F169 (≠ S172), S170 (≠ A173), L190 (= L193), G192 (= G195), I193 (= I196), T194 (≠ S197), T230 (≠ A233)
6snlD (R)-selective amine transaminase from exophiala sideris (see paper)
24% identity, 82% coverage: 40:244/249 of query aligns to 70:284/320 of 6snlD
- active site: K178 (= K134), E211 (= E169), L233 (= L193)
- binding pyridoxal-5'-phosphate: R76 (= R46), K178 (= K134), E211 (= E169), G214 (≠ S172), F215 (≠ A173), L233 (= L193), G235 (= G195), V236 (≠ I196), T237 (≠ S197), T273 (≠ A233)
Sites not aligning to the query:
7p3tB Transaminase of gamma-proteobacterium (see paper)
25% identity, 90% coverage: 20:243/249 of query aligns to 27:258/299 of 7p3tB
- binding pyridoxal-5'-phosphate: R53 (= R46), K153 (= K136), R157 (≠ Y140), E186 (= E169), S187 (≠ T170), A188 (≠ S171), A189 (≠ S172), S190 (≠ A173), G210 (= G195), I211 (= I196), T212 (≠ S197), T248 (≠ A233)
Query Sequence
>WP_012674308.1 NCBI__GCF_000021545.1:WP_012674308.1
MEKFFLNGQPFNDLSLLRPLMYGEGVFETFRYKEKLPKYIDYHYKRLIKGASFLKIPPIT
KEDYLYYINQAVNSIEDKDLYIKTILLSEGNSYYPLQPYKSNLLVVVKPYKAINTPITLT
ISPYKVHSKDPLLKIKSTNYLRNILVKRYAQEKGFFDAIILNEDDCITETSSANIFWIKG
RYLYTPSLECGVLEGISRKKILEEGKNQGFVVIEGEFNLKDLKGANLIFLSNALHGIMKV
ESIDPEAFK
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory