SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012674346.1 NCBI__GCF_000021545.1:WP_012674346.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9JZG1 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13 from Neisseria meningitidis serogroup B (strain MC58) (see 2 papers)
54% identity, 97% coverage: 3:500/516 of query aligns to 6:501/517 of Q9JZG1

query
sites
Q9JZG1

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D16 (= D13) binding Mn(2+)
H204 (= H201) binding Mn(2+)
H206 (= H203) binding Mn(2+)
N240 (= N237) binding Mn(2+)

Sites not aligning to the query:

366:517 Required for the condensation reaction. Not required to bind substrate

3rmjB Crystal structure of truncated alpha-isopropylmalate synthase from neisseria meningitidis (see paper)
63% identity, 62% coverage: 3:320/516 of query aligns to 3:306/308 of 3rmjB

query
sites
3rmjB

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active site: Q16 (= Q16)
binding manganese (ii) ion: D13 (= D13), H201 (= H201), H203 (= H203), N237 (= N237)

6e1jA Crystal structure of methylthioalkylmalate synthase (bjumam1.1) from brassica juncea (see paper)
49% identity, 73% coverage: 6:380/516 of query aligns to 20:407/409 of 6e1jA

query
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6e1jA

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binding coenzyme a: Q30 (= Q16), F60 (= F46), S63 (≠ A49), I95 (≠ L72), R97 (= R74), F121 (= F98), K132 (= K109), L133 (= L110), S322 (≠ A297), G323 (= G298), I324 (= I299), D327 (≠ H302), K331 (≠ A306), L359 (≠ H331), R362 (= R334), H363 (= H335)
binding 4-(methylsulfanyl)-2-oxobutanoic acid: P192 (= P168), T194 (= T170), H225 (= H201), H227 (= H203)
binding manganese (ii) ion: D27 (= D13), V82 (vs. gap), E84 (vs. gap), H225 (= H201), H227 (= H203)

Q9FG67 Methylthioalkylmalate synthase 1, chloroplastic; 2-isopropylmalate synthase 3; EC 2.3.3.17 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
48% identity, 73% coverage: 6:380/516 of query aligns to 87:474/506 of Q9FG67

query
sites
Q9FG67

I

V

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F

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T

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E

A

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I

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K

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I

A

A

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N

N

-

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-

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-

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A

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Y

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N

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D

A

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x
A

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C

C

H

H

N

N

D

D

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L

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G

L

L

A

A

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A

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I

A

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A

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G

A

A

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A

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T

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V

I

N

N

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I

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G

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N

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Y

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A

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K

A

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I

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A

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C

F

F

A

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I

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I

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A

P

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E

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D

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-

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K

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G

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-

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E

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D

D

A

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F

F

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L

L

A

T

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F

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D

D

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E

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A

A

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L

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S102 (= S21) mutation to F: In gsm1-1; loss of conversion of C3 to C4 glucosinolates.
A290 (≠ S199) mutation to T: In gsm1-2; loss of conversion of C3 to C4 glucosinolates.

Q9FN52 Methylthioalkylmalate synthase 3, chloroplastic; 2-isopropylmalate synthase 2; Methylthioalkylmalate synthase-like; EC 2.3.3.17 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
48% identity, 73% coverage: 6:381/516 of query aligns to 87:475/503 of Q9FN52

query
sites
Q9FN52

I

V

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G263 (= G172) mutation to E: In gsm2-1; loss of activity and lack of C6, C7 and C8 aliphatic glucosinolates.

6ktqA Crystal structure of catalytic domain of homocitrate synthase from sulfolobus acidocaldarius (sahcs(dram)) in complex with alpha- ketoglutarate/zn2+/coa (see paper)
36% identity, 74% coverage: 3:383/516 of query aligns to 21:388/399 of 6ktqA

query
sites
6ktqA

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K

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D

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A

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H

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H
|
H

N

N

D

D

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L

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A

A

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A

A

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L

A

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A

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F

I

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N

A

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D

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K

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E

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E

binding 2-oxoglutaric acid: R30 (= R12), R154 (≠ E136), T156 (≠ S138), E158 (= E140), S184 (≠ N166), T188 (= T170), H216 (= H201), H218 (= H203)
binding coenzyme a: V67 (≠ A49), R96 (= R74), A97 (= A75), F116 (= F98), H128 (≠ L110), E158 (= E140)
binding zinc ion: E31 (≠ D13), H216 (= H201), H218 (= H203)

Q8F3Q1 (R)-citramalate synthase CimA; LiCMS; EC 2.3.3.21 from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) (see 2 papers)
31% identity, 90% coverage: 3:466/516 of query aligns to 7:472/516 of Q8F3Q1

query
sites
Q8F3Q1

K

R

L

L

I

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I

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F

L

D

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D

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F

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E

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E

W

V

A

K

A

N

T

A

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Q

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L

C

T

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A

I

R

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A

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L

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G

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x
F

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D

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K

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A

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L

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I

R16 (= R12) mutation R->K,Q: Loss of activity.
RD 16:17 (= RD 12:13) binding pyruvate
D17 (= D13) mutation to A: 34-fold increase in Km for pyruvate and 315-fold decrease in kcat.; mutation to N: 4.4-fold increase in Km for pyruvate and 480-fold decrease in kcat.
L81 (= L76) mutation to A: 4.7-fold increase in Km for pyruvate and 15.7-fold decrease in kcat.; mutation to V: 3.3-fold increase in Km for pyruvate and 10.1-fold decrease in kcat.
F83 (vs. gap) mutation to A: 5-fold increase in Km for acetyl-CoA and 120-fold decrease in kcat.
L104 (≠ F98) mutation to V: 1.8-fold increase in Km for pyruvate and 3.4-fold decrease in kcat.
Y144 (≠ S138) binding pyruvate; mutation to L: 259-fold increase in Km for pyruvate and 76-fold decrease in kcat.; mutation to V: 114-fold increase in Km for pyruvate and 5.3-fold decrease in kcat.
E146 (= E140) mutation E->D,Q: Minor effects on the binding of acetyl-CoA, but causes a strong decrease in kcat.
T179 (= T170) binding pyruvate; mutation to A: 16.4-fold increase in Km for pyruvate and 186-fold decrease in kcat.
H302 (= H300) mutation H->A,N: Loss of activity.
D304 (≠ H302) mutation to A: 5.2-fold increase in Km for acetyl-CoA and 16.6-fold decrease in kcat.
N310 (≠ R308) mutation to A: 2.2-fold increase in Km for acetyl-CoA and 1.7-fold decrease in kcat.
L311 (≠ E309) mutation to A: 8-fold increase in Km for acetyl-CoA and 6-fold decrease in kcat.
Y312 (≠ T310) mutation to A: Loss of activity.
Y430 (≠ I429) mutation to L: No change in Km for acetyl-CoA and 2.3-fold decrease in kcat. Severely impairs inhibition by isoleucine.
D431 (= D430) mutation to A: 1.8-fold decrease in Km for acetyl-CoA and 5-fold decrease in kcat.
L451 (= L446) mutation to V: 1.5-fold increase in Km for acetyl-CoA and 4.3 decrease in kcat.
Y454 (= Y449) mutation to A: 1.4 decrease in Km for acetyl-CoA and 17-fold decrease in kcat. Still inhibited by isoleucine and weakly inhibited by leucine.
I458 (≠ S453) mutation to A: 1.3-fold decrease in Km for acetyl-CoA and 14-fold decrease in kcat. Abolishes inhibition by isoleucine.
T464 (vs. gap) mutation to A: 1.8-fold decrease in Km for acetyl-CoA and 4.3-fold decrease in kcat.
V468 (≠ G462) mutation to A: No change in Km for acetyl-CoA and 2-fold decrease in kcat. Increases inhibition by isoleucine and leucine becomes an effective inhibitor.

Sites not aligning to the query:

493 P→A: 1.5-fold decrease in Km for acetyl-CoA and 2.6-fold decrease in kcat.
495 Q→A: 1.6-fold decrease in Km for acetyl-CoA and 2.8-fold decrease in kcat.

4ov4A Isopropylmalate synthase binding with ketoisovalerate (see paper)
33% identity, 72% coverage: 6:379/516 of query aligns to 6:377/379 of 4ov4A

query
sites
4ov4A

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active site: Q16 (= Q16)
binding 3-methyl-2-oxobutanoic acid: R12 (= R12), P174 (= P168), T176 (= T170), H205 (= H201), H207 (= H203)
binding zinc ion: D13 (= D13), H205 (= H201), H207 (= H203)

4ov9A Structure of isopropylmalate synthase binding with alpha- isopropylmalate (see paper)
32% identity, 72% coverage: 6:379/516 of query aligns to 6:379/380 of 4ov9A

query
sites
4ov9A

I

I

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H

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H

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D

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A

A

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A

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active site: Q16 (= Q16)
binding (2S)-2-hydroxy-2-(propan-2-yl)butanedioic acid: R12 (= R12), L73 (= L72), T176 (= T170), H205 (= H201), H207 (= H203)
binding zinc ion: D13 (= D13), H205 (= H201), H207 (= H203)

Q9Y823 Homocitrate synthase, mitochondrial; HCS; EC 2.3.3.14 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see 2 papers)
30% identity, 73% coverage: 6:382/516 of query aligns to 37:401/418 of Q9Y823

query
sites
Q9Y823

I

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H

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x
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x
Q

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R43 (= R12) binding 2-oxoglutarate; mutation R->A,K,Q: Abolishes the catalytic activity.
E44 (≠ D13) binding 2-oxoglutarate; binding L-lysine; binding Zn(2+)
Q47 (= Q16) mutation to A: Abolishes the catalytic activity.
E74 (= E43) mutation to A: Abolishes the catalytic activity.; mutation to Q: Results in a moderate decrease in the turnover number and a slight increase in the Km value for each substrate.
H103 (≠ A75) binding 2-oxoglutarate; mutation to A: Substantially impairs catalytic efficiency.
D123 (≠ H96) binding L-lysine; mutation to N: Does not affect the catalytic activity but impairs L-lysine inhibition.
R163 (≠ E136) binding 2-oxoglutarate; mutation R->A,Q: Abolishes the catalytic activity.; mutation to K: Severely diminishes affinity for 2-oxoglutarate and substantially impairs catalytic efficiency.
S165 (= S138) binding 2-oxoglutarate; mutation to A: Results in a moderate decrease in catalytic efficiency.
E167 (= E140) mutation E->A,Q: Abolishes the catalytic activity.
T197 (= T170) binding 2-oxoglutarate; binding L-lysine; mutation to A: Exhibits a 25-fold decrease in catalytic efficiency.; mutation to S: Results in a modest decrease in catalytic efficiency.; mutation to V: Abolishes the catalytic activity.
E222 (≠ S199) mutation to Q: Does not affect the catalytic activity but impairs L-lysine inhibition.
H224 (= H201) binding 2-oxoglutarate; binding Zn(2+)
H226 (= H203) binding 2-oxoglutarate; binding Zn(2+)
R288 (≠ K264) mutation to K: Does not affect the catalytic activity but impairs L-lysine inhibition.
Y332 (= Y311) mutation to A: Abolishes the catalytic activity.; mutation to F: Results in a decrease in catalytic efficiency.
Q364 (≠ E343) mutation to R: Does not affect the catalytic activity but impairs L-lysine inhibition.

3ivtB Homocitrate synthase lys4 bound to 2-og (see paper)
30% identity, 73% coverage: 6:382/516 of query aligns to 32:396/400 of 3ivtB

query
sites
3ivtB

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active site: Q42 (= Q16)
binding 2-oxoglutaric acid: R38 (= R12), H98 (≠ A75), R158 (≠ E136), S160 (= S138), T192 (= T170), H219 (= H201), H221 (= H203)
binding zinc ion: E39 (≠ D13), H219 (= H201), H221 (= H203)

3mi3A Homocitrate synthase lys4 bound to lysine (see paper)
31% identity, 72% coverage: 6:374/516 of query aligns to 14:360/370 of 3mi3A

query
sites
3mi3A

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x
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I

A

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D

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D

active site: Q24 (= Q16)
binding lysine: R20 (= R12), D100 (≠ H96), T163 (= T170), H190 (= H201), H192 (= H203)
binding zinc ion: E21 (≠ D13), H190 (= H201), H192 (= H203)

3bliA Crystal structure of the catalytic domain of licms in complexed with pyruvate and acetyl-coa (see paper)
32% identity, 59% coverage: 3:308/516 of query aligns to 1:306/311 of 3bliA

query
sites
3bliA

K

R

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L

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A

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K

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G

N

V

V

D

D

V

R

I

V

E

E

A

I

G

A

F

S

A

A

A
x
R

A
x
V

S

S

E

K

G

G

D

E

F

L

E

E

A

T

V

V

K

Q

R

K

V

I

A

M

E

E

E

W

V

A

K

A

N

T

A

E

I

Q

V

L

C

T

S

E

L

R

A

I

R

E

A

I

L

L

Q

G

-

F

S

V

D

D

I

G

D

N

K

K

A

T

G

V

E

D

A

W

L

I

L

K

A

D

A

S

E

G

N

A

R

K

R

V

I

L

H

N

T

L

F

L

I

T

A

K

T

G

S

S

P

L

I

H

H

H

M

L

Q

E

Y

K

K

Q

L

L

K

G

M

K

T

T

P

P

D

K

E

E

V

F

E

T

R

D

A

V

V

S

A

F

A

V

V

I

K

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Y

Y

A

A

L

I

K

K

Y

S

T

G

D

L

D

K

V

I

E

N

F

V

S
x
Y

A

L

E
|
E

D

D

-

W

-

S

-

N

A

G

F

F

R

R

S

N

E

S

R

P

E

D

F

Y

L

V

Y

K

R

S

V

L

F

V

E

E

A

H

V

L

I

S

K

K

A

E

G

H

A

I

K

E

T

R

I

I

N

F

V

L

P
|
P

D

D

T
|
T

V

L

G

G

Y

V

A

L

I

S

P

P

E

E

F

T

G

F

Q

Q

L

G

I

V

K

D

D

S

I

L

K

I

N

Q

N

K

V

Y

P

P

N

D

I

I

D

H

K

-

A

-

V

-

I

F

S

E

V

F

H
|
H

C

G

H
|
H

N

N

D

D

L

Y

G

D

L

L

A

S

V

V

A

A

N

N

S

S

L

L

A

Q

A

A

V

I

K

R

N

A

G

G

A

V

R

K

Q

G

V

L

H

H

A

A

T

S

V

I

N

N

G

G

I

L

G

G

E

E

R

R

A

A

G

G

N

N

A

T

A

P

V

L

E

E

E

A

V

L

V

V

M

T

A

T

I

I

K

-

V

-

R

-

H

H

D

D

Y

K

F

S

K

N

G

S

I

-

Y

K

T

T

T

N

I

I

N

N

T

E

K

I

E

A

I

I

Y

T

K

E

T

A

S

S

R

R

L

L

L

V

C

E

R

V

I

F

T

S

G

G

S

K

F

R

V

I

Q

S

P

A

N

N

K

R

A

P

I

I

V

V

G

G

D

E

N

D

A

V

F

F

A

T

H

Q

E

T

A

A

G

G

I

V

H

N

Q

L

H

Y

G

A

-

N

-

P

I

I

L

L

A

P

H

E

R

R

active site: Q14 (= Q16)
binding acetyl coenzyme *a: Q14 (= Q16), R47 (≠ A48), V48 (≠ A49), E140 (= E140)
binding pyruvic acid: R10 (= R12), Y138 (≠ S138), P171 (= P168), T173 (= T170)
binding zinc ion: D11 (= D13), H201 (= H201), H203 (= H203)

O87198 Homocitrate synthase; HCS; EC 2.3.3.14 from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see paper)
31% identity, 72% coverage: 1:374/516 of query aligns to 1:365/376 of O87198

query
sites
O87198

M

M

E

R

K

E

L

W

I

K

I

I

F

I

D

D

T

S

T

T

L

L

R
|
R

D
x
E

G

G

E

E

Q

Q

A

F

P

E

G

K

F

A

S

N

M

F

T

S

V

T

E

Q

E

D

K

K

V

V

K

E

M

I

A

A

L

K

Q

A

L

L

E

D

K

E

L

F

G

G

V

I

D

E

V

Y

I

I

E

E

A

V

G

T

F

T

A

P

A

V

A

A

S

S

E

P

G

Q

D

S

F

R

E

K

A

D

V

A

K

E

R

V

V

L

A

A

E

S

E

L

V

G

K

L

N

K

A

A

I

K

V

V

C

V

S

T

L

-

A

-

R

-

A
x
H

L

I

Q

Q

S

C

D

R

I

L

D

D

K

A

A

A

G

K

E

V

A

A

L

V

L

E

A

T

A

G

E

-

N

V

R

Q

R

G

I

I

H
x
D

T

L

F

L

I

F

A

G

T

T

S

S

P

K

I

Y

H

L

M

R

Q

A

Y

A

K

H

L

G

K

R

M

D

T

I

P

P

D

-

E

R

V

I

E

E

R

E

A

A

V

K

A

E

A

V

V

I

K

A

Y

Y

A

I

L

R

K

E

Y

A

T

A

D

P

D

H

V

V

E

E

-

V

-
x
R

F

F

S
|
S

A

A

E

E

D

D

A

T

F

F

R

R

S

S

E

E

R

E

E

Q

F

D

L

L

Y

L

R

A

V

V

F

Y

E

E

A

A

V

V

I

A

K

P

A

Y

G

-

A

V

K

D

T

R

I

V

N

G

V

L

P

A

D

D

T
|
T

V

V

G

G

Y

V

A

A

I

T

P

P

E

R

E

Q

F

V

G

Y

Q

A

L

L

I

V

K

R

D

E

I

V

K

R

N

R

N

V

V

V

-

G

P

P

N

R

I

V

D

D

K

-

A

-

V

-

I

I

S

E

V

F

H
|
H

C

G

H
|
H

N

N

D

D

L

T

G

G

L

C

A

A

V

I

A

A

N

N

S

A

L

Y

A

E

A

A

V

I

K

E

N

A

G

G

A

A

R

T

Q

H

V

V

H

D

A

T

T

T

V

I

N

L

G

G

I

I

G

G

E

E

R

R

A

N

G

G

N

I

A

T

A

P

V

L

E

G

V

F

V

L

M

A

A

R

I

M

-

Y

K

T

V

L

R

Q

H

P

D

E

Y

Y

F

V

K

R

G

R

I

K

Y

Y

T

K

T

L

I

E

N

M

T

L

K

P

E

E

I

L

Y

-

K

-

T

-

S

D

R

R

L

M

L

V

C

A

R

R

I

M

T

V

G

G

S

V

F

E

V

I

Q

P

P

F

N

N

K

N

A

Y

I

I

V

T

G

G

D

E

N

T

A

A

F

F

A

S

H

H

E

K

A

A

G

G

I

M

H

H

Q

L

H

K

G

A

I

I

L

Y

A

I

H

N

R

P

E

E

T

A

Y

Y

E

E

I

P

M

Y

R

P

A

P

E

E

D

V

V

F

G

G

V

V

P

K

K

R

S

K

K

L

I

I

V

I

L

A

G

S

K

R

H

L

S

T

G

G

R

R

H

H

A

A

F

I

K

K

T

A

R

R

L

A

Q

E

E

E

L

L

G

G

Y

-

T

L

N

H

L

Y

S

G

D

E

A

E

E

E

I

L

D

H

S

R

L

V

F

T

L

Q

K

H

F

I

K

K

K

A

L

L

A

A

D

D

K

R

K

G

K

Q

E

L

V

T

F

L

D

E

E

E

R12 (= R12) binding 2-oxoglutarate
E13 (≠ D13) binding Mg(2+)
H72 (≠ A75) binding 2-oxoglutarate; mutation to L: Significant decrease in sensitivity to lysine inhibition. Large decrease in affinity for 2-oxoglutarate. Almost no effect on affinity for acetyl-CoA and on turnover number.
D92 (≠ H96) binding L-lysine
R133 (vs. gap) binding 2-oxoglutarate
S135 (= S138) binding L-lysine
T166 (= T170) binding 2-oxoglutarate; binding L-lysine
H195 (= H201) binding Mg(2+)
H197 (= H203) binding Mg(2+)

3ivsA Homocitrate synthase lys4 (see paper)
30% identity, 72% coverage: 6:375/516 of query aligns to 14:356/364 of 3ivsA

query
sites
3ivsA

I

I

F

I

D

E

T

S

T

T

L

L

R

R

D
x
E

G

G

E

E

Q
|
Q

A

F

P

A

G

N

F

A

S

F

M

F

T

D

V

T

E

E

E

K

K

K

V

I

K

Q

M

I

A

A

L

K

Q

A

L

L

E

D

K

N

L

F

G

G

V

V

D

D

V

Y

I

I

E

E

A

L

G

T

F

S

A

P

A

V

A

A

S

S

E

E

G

Q

-

S

-

R

-

Q

D

D

F

C

E

E

A

A

V

I

K

-

R

-

V

-

A

-

E

-

V

C

K

K

N

L

A

G

I

L

V

K

C

C

S

K

L

I

A

L

R

T

A

H

L

I

Q

R

S

C

D

H

I

M

D

D

K

D

A

A

G

R

E

V

A

A

L

V

L

E

A

T

A

G

E

V

N

D

R

G

R

-

I

V

H

D

T

V

F

V

I

I

A

G

T

T

S

T

P

Y

I

I

H

-

M

-

Q

-

Y

-

K

-

L

-

K

-

M

-

T

-

P

-

D

-

E

-

V

-

V

I

E

D

R

S

A

A

V

T

A

E

A

V

V

I

K

N

Y

F

A

V

L

K

K

S

Y

K

T

G

D

I

D

E

V

V

E

R

F

F

S

S

A

S

E

E

D

D

A

S

F

F

R

R

S

S

E

D

R

L

E

V

F

D

L

L

Y

L

R

S

V

L

F

Y

E

K

A

A

V

V

I

D

K

K

A

I

G

G

A

V

K

N

T

R

I

V

N

G

V

I

P

A

D

D

T

T

V

V

G

G

Y

C

A

A

I

T

P

P

E

R

E

Q

F

V

G

Y

Q

D

L

L

I

I

K

R

D

T

I

L

K

R

N

G

N

V

V

V

P

-

N

-

I

-

D

-

K

S

A

C

V

D

I

I

S

E

V

C

H
|
H

C

F

H
|
H

N

N

D

D

L

T

G

G

L

M

A

A

V

I

A

A

N

N

S

A

L

Y

A

C

A

A

V

L

K

E

N

A

G

G

A

A

R

T

Q

H

V

I

H

D

A

T

T

S

V

I

N

L

G

G

I

I

G

G

E

E

R

R

A

N

G

G

N

I

A

T

A

P

V

L

E

G

E

A

V

L

M

A

A

R

I

M

K

Y

V

V

R

T

-

D

H

R

D

E

Y

Y

F

I

K

T

G

H

I

K

Y

Y

T

K

T

L

I

N

N

Q

T

L

K

R

E

E

I

L

Y

-

K

-

T

-

S

E

R

N

L

L

L

V

C

A

R

D

I

A

T

V

G

E

S

V

F

Q

V

I

Q

P

P

F

N

N

K

N

A

Y

I

I

V

T

G

G

D

M

N

C

A

A

F

F

A

T

H

H

E

K

A

A

G

G

I

I

H

H

Q

A

H

K

G

A

I

I

L

L

A

A

H

N

R

P

E

S

T

T

Y

Y

E

E

I

I

M

L

R

K

A

P

E

E

D

D

V

F

G

G

V

M

P

S

K

R

S

Y

K

V

I

H

V

V

L

G

G

S

K

R

H

L

S

T

G

G

R

W

H

N

A

A

F

I

K

K

T

S

R

R

L

A

Q

E

E

Q

L

L

G

-

Y

-

T

-

N

N

L

L

S

H

D

-

A

L

E

Q

I

A

D

K

S

E

L

L

F

T

L

V

K

R

F

I

K

K

L

L

A

A

D

V

K

R

K

T

K

L

E

A

V

M

F

D

D

D

E

V

D

D

active site: Q24 (= Q16)
binding cobalt (ii) ion: E21 (≠ D13), H188 (= H201), H190 (= H203)

2ztjA Crystal structure of homocitrate synthase from thermus thermophilus complexed with alpha-ketoglutarate (see paper)
30% identity, 63% coverage: 1:327/516 of query aligns to 1:311/312 of 2ztjA

query
sites
2ztjA

M

M

E

R

K

E

L

W

I

K

I

I

F

I

D

D

T

S

T

T

L

L

R
|
R

D
x
E

G

G

E

E

Q
|
Q

A

F

P

E

G

K

F

A

S

N

M

F

T

S

V

T

E

Q

E

D

K

K

V

V

K

E

M

I

A

A

L

K

Q

A

L

L

E

D

K

E

L

F

G

G

V

I

D

E

V

Y

I

I

E

E

A

V

G

T

F

T

A

P

A

V

A

A

S

S

-

P

-

Q

-

S

E

R

G

K

D

D

F

A

E

E

A

V

V

L

K

A

R

S

V

L

A

G

E

L

E

K

V

A

K

K

-

V

N

T

A
x
H

I

I

V

Q

C

C

S

R

L

L

-

D

A

A

R

A

A

K

L

V

Q

A

S

V

D

E

I

T

D

G

K

V

A

Q

G

G

E

I

A

D

L

L

L
|
L

A

F

A

G

E

T

N

G

R

R

R

D

I

I

H

P

T

R

F

I

I

I

A

-

T

-

S

-

P

-

I

-

H

-

M

-

Q

-

Y

-

K

-

L

-

K

-

M

-

T

-

P

-

D

-

E

E

V

E

V

A

E

K

R

E

A

V

V

I

A

A

A

Y

V

I

K

R

Y

E

A

A

L

A

K

P

Y

H

T

V

D

E

D

-

V

V

E
x
R

F

F

S

S

A

A

E

E

D

D

A

T

F

F

R

R

S

S

E

E

R

E

E

Q

F

D

L

L

Y

L

R

A

V

V

F

Y

E

E

A

A

V

V

I

A

K

P

A

Y

G

-

A

V

K

D

T

R

I

V

N

G

V

L

P
x
A

D

D

T
|
T

V

V

G

G

Y

V

A

A

I

T

P

P

E

R

E

Q

F

V

G

Y

Q

A

L

L

I

V

K

R

D

E

I

V

K

R

N

R

N

V

V

V

-

G

P

P

N

R

I

V

D

D

K

-

A

-

V

-

I

I

S

E

V

F

H
|
H

C

G

H
|
H

N

N

D

D

L

T

G

G

L

C

A

A

V

I

A

A

N

N

S

A

L

Y

A

E

A

A

V

I

K

E

N

A

G

G

A

A

R

T

Q

H

V

V

H

D

A

T

T

T

V

I

N

L

G

G

I

I

G

G

E

E

R

R

A

N

G

G

N

I

A

T

A

P

V

L

E

G

V

F

V

L

M

A

A

R

I

M

-

Y

K

T

V

L

R

Q

H

P

D

E

Y

Y

F

V

K

R

G

R

I

K

Y

Y

T

K

T

L

I

E

N

M

T

L

K

P

E

E

I

L

Y

-

K

-

T

-

S

D

R

R

L

M

L

V

C

A

R

R

I

M

T

V

G

G

S

V

F

E

V

I

Q

P

P

F

N

N

K

N

A

Y

I

I

V

T

G

G

D

E

N

T

A

A

F

F

A

S

H

H

E

K

A

A

G

G

I

M

H

H

Q

L

H

K

G

A

I

I

L

Y

A

I

H

N

R

P

E

E

T

A

Y

Y

E

E

I

P

M

Y

R

P

A

P

E

E

D

V

V

F

G

G

V

V

P

K

K

R

S

K

K

L

I

I

V

I

active site: Q16 (= Q16)
binding 2-oxoglutaric acid: R12 (= R12), H72 (≠ A67), L94 (= L88), R125 (≠ E136), A156 (≠ P168), T158 (= T170), H187 (= H201), H189 (= H203)
binding copper (ii) ion: E13 (≠ D13), H187 (= H201), H189 (= H203)

2zyfA Crystal structure of homocitrate synthase from thermus thermophilus complexed with magnesuim ion and alpha-ketoglutarate (see paper)
31% identity, 63% coverage: 1:327/516 of query aligns to 1:313/314 of 2zyfA

query
sites
2zyfA

M

M

E

R

K

E

L

W

I

K

I

I

F

I

D

D

T

S

T

T

L

L

R
|
R

D
x
E

G

G

E

E

Q
|
Q

A

F

P

E

G

K

F

A

S

N

M

F

T

S

V

T

E

Q

E

D

K

K

V

V

K

E

M

I

A

A

L

K

Q

A

L

L

E

D

K

E

L

F

G

G

V

I

D

E

V

Y

I

I

E

E

A

V

G

T

F

T

A

P

A

V

A

A

S

S

E

P

G

Q

D

S

F

R

E

K

A

D

V

A

K

E

R

V

V

L

A

A

E

S

E

L

V

G

K

L

N

K

A

A

I

K

V

V

C

V

S

T

L

-

A

-

R

-

A
x
H

L

I

Q

Q

S

C

D

R

I

L

D

D

K

A

A

A

G

K

E

V

A

A

L

V

L

E

A

T

A

G

E

-

N

V

R

Q

R

G

I

I

H

D

T

L

F
x
L

I

F

A

G

T

T

S

S

P

-

I

-

H

-

M

-

Q

-

Y

-

K

K

L

G

K

R

M

D

T

I

P

P

D

-

E

R

V

I

E

E

R

E

A

A

V

K

A

E

A

V

V

I

K

A

Y

Y

A

I

L

R

K

E

Y

A

T

A

D

P

D

H

V

V

E

E

-

V

-
x
R

F

F

S

S

A

A

E

E

D

D

A

T

F

F

R

R

S

S

E

E

R

E

E

Q

F

D

L

L

Y

L

R

A

V

V

F

Y

E

E

A

A

V

V

I

A

K

P

A

Y

G

-

A

V

K

D

T

R

I

V

N

G

V

L

P

A

D

D

T
|
T

V

V

G

G

Y

V

A

A

I

T

P

P

E

R

E

Q

F

V

G

Y

Q

A

L

L

I

V

K

R

D

E

I

V

K

R

N

R

N

V

V

V

-

G

P

P

N

R

I

V

D

D

K

-

A

-

V

-

I

I

S

E

V

F

H
|
H

C

G

H
|
H

N

N

D

D

L

T

G

G

L

C

A

A

V

I

A

A

N

N

S

A

L

Y

A

E

A

A

V

I

K

E

N

A

G

G

A

A

R

T

Q

H

V

V

H

D

A

T

T

T

V

I

N

L

G

G

I

I

G

G

E

E

R

R

A

N

G

G

N

I

A

T

A

P

V

L

E

G

V

F

V

L

M

A

A

R

I

M

-

Y

K

T

V

L

R

Q

H

P

D

E

Y

Y

F

V

K

R

G

R

I

K

Y

Y

T

K

T

L

I

E

N

M

T

L

K

P

E

E

I

L

Y

-

K

-

T

-

S

D

R

R

L

M

L

V

C

A

R

R

I

M

T

V

G

G

S

V

F

E

V

I

Q

P

P

F

N

N

K

N

A

Y

I

I

V

T

G

G

D

E

N

T

A

A

F

F

A

S

H

H

E

K

A

A

G

G

I

M

H

H

Q

L

H

K

G

A

I

I

L

Y

A

I

H

N

R

P

E

E

T

A

Y

Y

E

E

I

P

M

Y

R

P

A

P

E

E

D

V

V

F

G

G

V

V

P

K

K

R

S

K

K

L

I

I

V

I

active site: Q16 (= Q16)
binding 2-oxoglutaric acid: R12 (= R12), H72 (≠ A75), L94 (≠ F98), R127 (vs. gap), T160 (= T170), H189 (= H201)
binding magnesium ion: E13 (≠ D13), H189 (= H201), H191 (= H203)

3a9iA Crystal structure of homocitrate synthase from thermus thermophilus complexed with lys (see paper)
31% identity, 61% coverage: 6:321/516 of query aligns to 5:306/347 of 3a9iA

query
sites
3a9iA

I

I

F

I

D

D

T

S

T

T

L

L

R
|
R

D
x
E

G

G

E

E

Q

Q

A

F

P

E

G

K

F

A

S

N

M

F

T

S

V

T

E

Q

E

D

K

K

V

V

K

E

M

I

A

A

L

K

Q

A

L

L

E

D

K

E

L

F

G

G

V

I

D

E

V

Y

I

I

E

E

A

V

G

T

F

T

A

P

A

V

A

A

S

S

E

P

G

Q

D

S

F

R

E

K

A

D

V

A

K

E

R

V

V

L

A

A

E

S

E

L

V

G

K

L

N

K

A

A

I

K

V

V

C

V

S

T

L

-

A

-

R

-

A

H

L

I

Q

Q

S

C

D

R

I

L

D

D

K

A

A

A

G

K

E

V

A

A

L

V

L

E

A

T

A

G

E

-

N

V

R

Q

R

G

I

I

H
x
D

T

L

F

L

I

F

A

G

T

T

S

S

P

-

I

-

H

-

M

-

Q

-

Y

-

K

K

L

Y

K

L

M

D

T

I

P

P

D

-

E

R

V

I

E

E

R

E

A

A

V

K

A

E

A

V

V

I

K

A

Y

Y

A

I

L

R

K

E

Y

A

T

A

D

P

D

H

V

V

E

E

-

V

-

R

F

F

S
|
S

A

A

E

E

D

D

A

T

F

F

R

R

S

S

E

E

R

E

E

Q

F

D

L

L

Y

L

R

A

V

V

F

Y

E

E

A

A

V

V

I

A

K

P

A

Y

G

-

A

V

K

D

T

R

I

V

N

G

V

L

P

A

D

D

T
|
T

V

V

G

G

Y

V

A

A

I

T

P

P

E

R

E

Q

F

V

G

Y

Q

A

L

L

I

V

K

R

D

E

I

V

K

R

N

R

N

V

V

V

-

G

P

P

N

R

I

V

D

D

K

-

A

-

V

-

I

I

S

E

V

F

H
|
H

C

G

H
|
H

N

N

D

D

L

T

G

G

L

C

A

A

V

I

A

A

N

N

S

A

L

Y

A

E

A

A

V

I

K

E

N

A

G

G

A

A

R

T

Q

H

V

V

H

D

A

T

T

T

V

I

N

L

G

G

I

I

G

G

E

E

R

R

A

N

G

G

N

I

A

T

A

P

V

L

E

G

V

F

V

L

M

A

A

R

I

M

-

Y

K

T

V

L

R

Q

H

P

D

E

Y

Y

F

V

K

R

G

R

I

K

Y

Y

T

K

T

L

I

E

N

M

T

L

K

P

E

E

I

L

Y

-

K

-

T

-

S

D

R

R

L

M

L

V

C

A

R

R

I

M

T

V

G

G

S

V

F

E

V

I

Q

P

P

F

N

N

K

N

A

Y

I

I

V

T

G

G

D

E

N

T

A

A

F

F

A

S

H

H

E

K

A

A

G

G

I

M

H

H

Q

L

H

K

G

A

I

I

L

Y

A

I

H

N

R

P

E

E

T

A

Y

Y

E

E

I

P

M

Y

R

P

A

P

E

E

D

V

V

F

G

G

V

V

binding cobalt (ii) ion: E12 (≠ D13), H188 (= H201), H190 (= H203)
binding lysine: R11 (= R12), D91 (≠ H96), S128 (= S138), T159 (= T170), H188 (= H201), H190 (= H203)

3hpsA Crystal structure of mycobacterium tuberculosis leua complexed with ketoisocaproate (kic)
25% identity, 95% coverage: 11:500/516 of query aligns to 62:573/575 of 3hpsA

query
sites
3hpsA

L

L

R
|
R

D
|
D

G

G

E

N

Q

Q

A

A

P

L

G

I

F

D

S

P

M

M

T

S

V

P

E

A

E

R

K

K

V

R

K

R

M

M

A

F

L

D

Q

L

L

L

E

V

K

R

L

M

G

G

V

Y

D

K

V

E

I

I

E

E

A

V

G

G

F

F

A

P

A

S

A

A

S

S

E

Q

G

T

D

D

F

F

E

D

A

F

V

V

K

R

R

E

V

I

A

I

E

E

E

Q

-

G

-

A

-

I

V

P

K

D

N

D

A

V

I

T

V

I

C

Q

S

V

L

L

A

T

R

Q

A

C

L

R

Q

P

S

E

D

L

I

I

D

E

K

R

A

T

G

F

E

Q

A

A

L

C

L

S

A

G

A

A

E

P

N

R

R

A

R

I

I

V

H
|
H

T

F

F
x
Y

I

N

A

S

T

T

S

S

P

I

I

L

H

Q

M

R

Q

R

Y

V

K

V

L

F

K

R

M

A

T

N

P

R

D

A

E

E

V

V

-

Q

-

A

-

I

-

A

V

T

E

D

R

G

A

A

V

R

A

K

A

C

V

V

K

E

Y

Q

A

A

L

A

K

K

Y

Y

T

P

D

G

D

T

-

Q

-

W

-

R

V

F

E

E

F

Y

S

S

A

P

E

E

D

S

A

Y

F

T

R

G

S

T

E

E

R

L

E

E

F

Y

L

A

Y

K

R

Q

V

V

F

C

E

D

A

A

V

V

I

G

K

E

A

V

G

I

A

A

K

P

T

T

-

P

-

E

-

R

-

P

-

I

I

F

N

N

V

L

P
|
P

D

A

T
|
T

V

V

G

E

Y

M

A

T

I

T

P

P

E

N

E

V

F

Y

G

A

Q

D

L

S

I

I

K

E

D

W

I

M

K

S

N

R

N

N

V

L

P

A

N

N

I

R

D

E

K

S

A

V

V

I

I

L

S

S

V

L

H
|
H

C

P

H
|
H

N

N

D

D

L

R

G

G

L

T

A

A

V

V

A

A

N

A

S

A

L

E

A

L

A

G

V

F

K

A

N

A

G

G

A

A

R

D

Q

R

V

I

H

E

A

G

T

C

V

L

N

F

G

G

I

N

G

G

E

E

R

R

A

T

G

G

N

N

A

V

A

C

V

L

E

-

V

V

V

T

M

L

A

G

I

L

K

N

V

L

R

-

H

-

D

-

Y

F

F

S

K

R

G

G

I

V

Y

D

T

P

T

Q

I

I

N

D

T

F

K

S

E

N

I

I

Y

D

K

E

T

I

S

R

R

R

L

T

L

V

C

E

R

Y

I

C

T

N

G

Q

S

L

F

P

V

V

Q

H

P

E

N

R

K

H

A

P

I

Y

V

G

G

G

D

D

N

L

A

V

F

Y

A

T

H

A

E

F

A

S

G

G

I

S

H

H

Q

Q

H

D

G

A

I

I

-

N

-

K

-

G

-

L

-

D

-

A

-

M

-

K

-

L

-

D

-

A

-

D

-

A

-

D

-

C

-

D

-

V

-

D

L

M

A

L

H

W

R

Q

E

V

T

P

Y

Y

E

L

I

P

M

I

R

D

A

P

E

R

D

D

V

V

G

G

V

R

P

T

K

Y

S

E

K

A

I

V

V

I

L

K

G

G

K

G

H

-

S

-

G

-

R

-

H

-

A

-

F

-

K

-

T

-

R

-

L

-

Q

-

E

-

L

V

G

A

Y

Y

T

I

N

M

L

K

S

T

D

D

A

H

E

G

I

L

D

S

-

L

-

P

-

R

S

R

L

L

F

Q

L

I

K

E

F

F

K

S

K

Q

L

V

A

I

D

-

K

Q

K

K

K

I

E

E

V

V

F

S

D

P

E

K

D

E

I

M

E

W

A

D

L

A

I

F

L

A

E

E

F

Y

S

L

F

A

F

P

E

V

N

R

E

P

V

L

K

E

V

R

S

I

Y

R

F

Q

H

H

V

V

V

D

S

A

G

A

D

D

N

D

-

D

-

G

-

T

V

T

I

S

P

I

S

T

A

A

T

T

V

V

K

K

I

I

E

-

K

-

D

N

G

G

E

V

E

E

I

T

I

E

S

I

T

S

A

G

S

S

G

G

D

N

G
|
G

P
|
P

I
x
L

D
x
A

S

A

V

F

I

V

N

H

A

A

V

L

E

A

K

D

A

-

I

V

G

G

I

F

K

D

G

V

K

A

L

V

L

L

D

D

Y

Y

T

Y

I

E

R

H

S

A

L

M

S

S

S

A

G

G

K
x
D

D

D

A
|
A

M
x
Q

G

A

-

A

-

Y

-

V

E

E

V

A

R

S

V

V

L

T

V

I

K

A

F

T

D

S

D

K

T

T

D

V

Y

W

V

-

A

-

S

-

G

G

K

V

G

G

T

I

S

A

T
x
P

D

S

I
|
I

I

T

E

T

A

A

S

S

L

L

K

R

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binding 2-oxo-4-methylpentanoic acid: R63 (= R12), H150 (= H96), Y152 (≠ F98), P235 (= P168), T237 (= T170), H268 (= H201), H270 (= H203)
binding leucine: G500 (= G427), P501 (= P428), L502 (≠ I429), A503 (≠ D430), D530 (≠ K458), A532 (= A460), Q533 (≠ M461), P557 (≠ T484), I559 (= I486)
binding zinc ion: D64 (= D13), H268 (= H201), H270 (= H203)

3figB Crystal structure of leucine-bound leua from mycobacterium tuberculosis (see paper)
25% identity, 95% coverage: 11:500/516 of query aligns to 62:574/577 of 3figB

query
sites
3figB

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binding leucine: G501 (= G427), P502 (= P428), L503 (≠ I429), A504 (≠ D430), D531 (≠ K458), A533 (= A460), P558 (≠ T484), I560 (= I486)
binding zinc ion: D64 (= D13), H268 (= H201), H270 (= H203)

Query Sequence

>WP_012674346.1 NCBI__GCF_000021545.1:WP_012674346.1
MEKLIIFDTTLRDGEQAPGFSMTVEEKVKMALQLEKLGVDVIEAGFAAASEGDFEAVKRV
AEEVKNAIVCSLARALQSDIDKAGEALLAAENRRIHTFIATSPIHMQYKLKMTPDEVVER
AVAAVKYALKYTDDVEFSAEDAFRSEREFLYRVFEAVIKAGAKTINVPDTVGYAIPEEFG
QLIKDIKNNVPNIDKAVISVHCHNDLGLAVANSLAAVKNGARQVHATVNGIGERAGNAAV
EEVVMAIKVRHDYFKGIYTTINTKEIYKTSRLLCRITGSFVQPNKAIVGDNAFAHEAGIH
QHGILAHRETYEIMRAEDVGVPKSKIVLGKHSGRHAFKTRLQELGYTNLSDAEIDSLFLK
FKKLADKKKEVFDEDIEALILEEFSFFENEVKVSYFHVVSGDNVIPSATVKIEKDGEEII
STASGDGPIDSVINAVEKAIGIKGKLLDYTIRSLSSGKDAMGEVRVLVKFDDTDYVASGK
GTSTDIIEASLKAYVDAYNKYTARKAFLEKRISEGV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory