SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012674358.1 NCBI__GCF_000021545.1:WP_012674358.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 1 hits to proteins with known functional sites (download)

3al0C Crystal structure of the glutamine transamidosome from thermotoga maritima in the glutamylation state. (see paper)
31% identity, 96% coverage: 2:92/95 of query aligns to 2:92/564 of 3al0C

query
sites
3al0C

I

V

D

T

K

K

D

D

T

L

V

V

V

L

K

H

V

L

A

E

Q

N

L

L

S

A

K

R

L

L

K

E

L

L

N

S

E

E

N

D

E

Q

V

R

N

E

L

S

F

L

S

M

K

K

Q

D

F

F

N

Q

D

E

I

I

I

L

S

D

F

Y

V

V

E

E

K

L

L

L

K

N

E

E

V

V

D

D

T

V

E

E

G

G

V

V

P

E

P

P

F

M

Y

Y

E

T

L

P

N

V

L

E

E

D

E

S

G

A

I

K

Y

L

R

R

E

K

D

G

E

D

P

P

H

R

Q

F

S

F

I

E

S

M

N

R

E

D

E

L

A

I

L

K

S

K

N

N

A

F

P

P

Q

E

K

E

E

K

G

D

G

G

F

H

F

I

I

K

V

V

P

P

R

G

V

I

Sites not aligning to the query:

active site: 110, 335
binding o5'-(l-glutamyl-sulfamoyl)-adenosine: 106, 108, 109, 118, 122, 142, 276, 294, 295, 297, 298, 324, 325, 333
binding : 144, 145, 148, 208, 213, 252, 255, 266, 269, 270, 276, 297, 298, 299, 300, 301, 304, 330, 332, 363, 364, 365, 370, 387, 389, 391, 392, 397, 400, 407, 446, 447, 453, 457, 509, 520, 524, 527, 535, 536, 538, 539

Query Sequence

>WP_012674358.1 NCBI__GCF_000021545.1:WP_012674358.1
MIDKDTVVKVAQLSKLKLNENEVNLFSKQFNDIISFVEKLKEVDTEGVPPFYELNLEEGI
YREDEPHQSISNEEALSNAPQKEGGFFIVPRVVGQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory