SitesBLAST
Comparing WP_012674632.1 NCBI__GCF_000021545.1:WP_012674632.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P14169 CDP-paratose 2-epimerase; CDP-tyvelose 2-epimerase; EC 5.1.3.10 from Salmonella typhi (see paper)
61% identity, 99% coverage: 1:338/342 of query aligns to 1:337/338 of P14169
- T124 (≠ S124) binding substrate
1orrA Crystal structure of cdp-tyvelose 2-epimerase complexed with NAD and cdp (see paper)
61% identity, 99% coverage: 2:338/342 of query aligns to 2:337/338 of 1orrA
- active site: T124 (≠ S124), N125 (= N125), K126 (= K126), Y164 (= Y164), K168 (= K168)
- binding cytidine-5'-diphosphate: K126 (= K126), S193 (= S193), Y203 (= Y203), W207 (= W207), W210 (= W210), F211 (= F211), S229 (≠ H230), Q234 (= Q235), R236 (= R237), R298 (= R299), D301 (= D302)
- binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), L12 (= L12), D31 (= D31), N32 (= N32), S34 (≠ Y34), R35 (= R35), D57 (= D57), I58 (≠ V58), L79 (= L79), V83 (= V83), S122 (= S122), Y164 (= Y164), K168 (= K168), H191 (= H191), M194 (= M194), Q205 (= Q205)
4zrnA Crystal structure of udp-glucose 4-epimerase (tm0509) with udp-glucose from hyperthermophilic eubacterium thermotoga maritima (see paper)
30% identity, 98% coverage: 1:335/342 of query aligns to 1:303/309 of 4zrnA
- active site: T117 (≠ S123), G119 (≠ N125), A120 (≠ K126), Y143 (= Y164), K147 (= K168), Y181 (≠ T202), G185 (= G206)
- binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (≠ L12), D31 (= D31), N32 (= N32), S34 (= S38), S35 (≠ Y39), G36 (≠ A40), S51 (≠ D57), I52 (≠ V58), L73 (= L79), A74 (= A80), A75 (≠ G81), T92 (≠ V98), S115 (≠ Y121), S116 (= S122), Y143 (= Y164), K147 (= K168), Y170 (≠ H191), V173 (≠ M194)
- binding uridine-5'-diphosphate-glucose: T117 (≠ S123), G119 (≠ N125), A120 (≠ K126), Y143 (= Y164), N172 (≠ S193), G185 (= G206), V186 (≠ W207), H201 (≠ T228), F203 (≠ H230), Y208 (≠ Q235), R210 (= R237), V244 (≠ L274), R267 (= R299), D270 (= D302)
1kewA The crystal structure of dtdp-d-glucose 4,6-dehydratase (rmlb) from salmonella enterica serovar typhimurium with thymidine diphosphate bound (see paper)
29% identity, 98% coverage: 1:335/342 of query aligns to 1:333/361 of 1kewA
- active site: T133 (≠ S124), D134 (≠ N125), E135 (≠ K126), L152 (≠ F148), L154 (≠ R151), F155 (= F152), T158 (≠ S155), Y167 (= Y164), K171 (= K168)
- binding nicotinamide-adenine-dinucleotide: G10 (= G10), F11 (= F11), I12 (≠ L12), D32 (= D31), K33 (≠ N32), L34 (= L33), T35 (≠ Y34), A37 (≠ V36), G38 (= G37), D58 (= D57), I59 (≠ V58), L80 (= L79), A81 (= A80), A82 (≠ G81), S84 (≠ V83), T99 (≠ V98), I131 (≠ S122), S132 (= S123), T133 (≠ S124), Y167 (= Y164), K171 (= K168), C194 (≠ H191), N196 (≠ S193), N197 (≠ M194)
- binding thymidine-5'-diphosphate: E135 (≠ K126), N196 (≠ S193), K206 (≠ G206), L207 (≠ W207), P222 (≠ T228), Y224 (≠ H230), R231 (= R237), N266 (vs. gap), R297 (= R299), H300 (≠ D302)
Sites not aligning to the query:
1keuA The crystal structure of dtdp-d-glucose 4,6-dehydratase (rmlb) from salmonella enterica serovar typhimurium with dtdp-d-glucose bound (see paper)
29% identity, 98% coverage: 1:335/342 of query aligns to 1:333/361 of 1keuA
- active site: T133 (≠ S124), D134 (≠ N125), E135 (≠ K126), L152 (≠ F148), L154 (≠ R151), F155 (= F152), T158 (≠ S155), Y167 (= Y164), K171 (= K168)
- binding 2'deoxy-thymidine-5'-diphospho-alpha-d-glucose: S84 (≠ V83), T133 (≠ S124), D134 (≠ N125), E135 (≠ K126), Y167 (= Y164), N196 (≠ S193), K206 (≠ G206), L207 (≠ W207), P222 (≠ T228), Y224 (≠ H230), R231 (= R237), N266 (vs. gap), R297 (= R299), H300 (≠ D302)
- binding nicotinamide-adenine-dinucleotide: G10 (= G10), F11 (= F11), I12 (≠ L12), D32 (= D31), K33 (≠ N32), L34 (= L33), T35 (≠ Y34), G38 (= G37), D58 (= D57), L80 (= L79), A81 (= A80), A82 (≠ G81), S84 (≠ V83), T99 (≠ V98), S132 (= S123), T133 (≠ S124), Y167 (= Y164), K171 (= K168), C194 (≠ H191), N196 (≠ S193), N197 (≠ M194)
Sites not aligning to the query:
P26391 dTDP-glucose 4,6-dehydratase; EC 4.2.1.46 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
29% identity, 98% coverage: 1:335/342 of query aligns to 1:333/361 of P26391
- FI 11:12 (≠ FL 11:12) binding NAD(+)
- DKLT 32:35 (≠ DNLY 31:34) binding NAD(+)
- DI 58:59 (≠ DV 57:58) binding NAD(+)
- LAAES 80:84 (≠ LAGQV 79:83) binding NAD(+)
- S84 (≠ V83) binding substrate
- T99 (≠ V98) binding NAD(+)
- T133 (≠ S124) binding substrate
- YSASK 167:171 (≠ YGCSK 164:168) binding NAD(+)
- N196 (≠ S193) binding substrate
- N197 (≠ M194) binding NAD(+)
- KL 206:207 (≠ GW 206:207) binding substrate
- PIY 222:224 (≠ TIH 228:230) binding substrate
- R231 (= R237) binding substrate
- N266 (vs. gap) binding substrate
- DRPGH 296:300 (≠ PRKSD 298:302) binding substrate
Sites not aligning to the query:
8du1A Crystal structure of NAD bound dtdp-glucose 4,6-dehydratase from elizabethkingia anophelis
29% identity, 97% coverage: 4:335/342 of query aligns to 7:336/361 of 8du1A
- binding nicotinamide-adenine-dinucleotide: G10 (= G7), G13 (= G10), F14 (= F11), I15 (≠ L12), D36 (= D31), A37 (≠ N32), L38 (= L33), T39 (≠ Y34), G42 (= G37), D62 (= D57), I63 (≠ V58), L84 (= L79), A85 (= A80), A86 (≠ G81), T103 (≠ V98), S143 (= S123), T144 (≠ S124), Y169 (= Y164), K173 (= K168), C196 (≠ H191)
6bwlA X-ray structure of pal from bacillus thuringiensis (see paper)
28% identity, 99% coverage: 1:338/342 of query aligns to 1:311/313 of 6bwlA
- active site: T122 (≠ S124), C123 (≠ N125), M124 (≠ K126), Y147 (= Y164), K151 (= K168)
- binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (≠ L12), D31 (= D31), N32 (= N32), L33 (= L33), N35 (≠ R35), S36 (≠ V36), D57 (= D57), I58 (≠ V58), L79 (= L79), A80 (= A80), A81 (≠ G81), I83 (≠ V83), M120 (≠ S122), K151 (= K168), N176 (≠ S193), T177 (≠ M194)
- binding uridine-5'-diphosphate: N176 (≠ S193), G189 (= G206), V190 (≠ W207), N205 (≠ T228), I206 (= I229), Y207 (≠ H230), Q212 (= Q235), R214 (= R237), I250 (≠ L276), E275 (≠ S301)
7ystA Crystal structure of udp-glucose 4-epimerase (rv3634c) in complex with both udp-glucose and udp-galactose in chain b from mycobacterium tuberculosis
27% identity, 98% coverage: 1:335/342 of query aligns to 1:307/312 of 7ystA
- binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (≠ L12), D31 (= D31), N32 (= N32), T35 (≠ V36), G36 (= G37), D56 (= D57), I57 (≠ V58), L77 (= L79), A78 (= A80), A79 (≠ G81), I81 (≠ V83), V96 (= V98), T119 (≠ Y121), Y146 (= Y164), K150 (= K168), P173 (≠ H191), A174 (≠ S192), N175 (≠ S193), V176 (≠ M194)
- binding uridine-5'-diphosphate-glucose: I81 (≠ V83), R84 (≠ T86), S121 (= S123), G123 (≠ N125), Y146 (= Y164), A174 (≠ S192), N175 (≠ S193), A187 (≠ Q205), G188 (= G206), V189 (≠ W207), F193 (= F211), R204 (≠ T228), V205 (≠ I229), F206 (≠ H230), R213 (= R237), D248 (≠ L274), R271 (= R299)
7ysmA Crystal structure of udp-glucose 4-epimerase (rv3634c) co-crystallized with udp-n-acetylglucosamine from mycobacterium tuberculosis
27% identity, 98% coverage: 1:335/342 of query aligns to 1:307/311 of 7ysmA
- binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (≠ L12), D31 (= D31), N32 (= N32), T35 (≠ V36), G36 (= G37), D56 (= D57), I57 (≠ V58), L77 (= L79), A78 (= A80), A79 (≠ G81), I81 (≠ V83), T119 (≠ Y121), Y146 (= Y164), K150 (= K168), P173 (≠ H191), N175 (≠ S193), V176 (≠ M194)
- binding uridine-diphosphate-n-acetylgalactosamine: I81 (≠ V83), R84 (≠ T86), S121 (= S123), G123 (≠ N125), S124 (≠ K126), Y146 (= Y164), A174 (≠ S192), N175 (≠ S193), G188 (= G206), V189 (≠ W207), F193 (= F211), R204 (≠ T228), V205 (≠ I229), F206 (≠ H230), N211 (≠ Q235), R213 (= R237), D248 (≠ L274), R271 (= R299)
7ys9A Crystal structure of udp-glucose 4-epimerase (rv3634c) in complex with both udp-glucose and udp-galactose in chaina from mycobacterium tuberculosis
27% identity, 98% coverage: 1:335/342 of query aligns to 1:307/310 of 7ys9A
- binding galactose-uridine-5'-diphosphate: I81 (≠ V83), R84 (≠ T86), S121 (= S123), G123 (≠ N125), Y146 (= Y164), A174 (≠ S192), N175 (≠ S193), A187 (≠ Q205), G188 (= G206), V189 (≠ W207), F193 (= F211), R204 (≠ T228), F206 (≠ H230), N211 (≠ Q235), R213 (= R237), D248 (≠ L274), R271 (= R299)
- binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (≠ L12), D31 (= D31), N32 (= N32), A34 (≠ R35), T35 (≠ V36), G36 (= G37), D56 (= D57), I57 (≠ V58), L77 (= L79), A78 (= A80), A79 (≠ G81), I81 (≠ V83), T119 (≠ Y121), Y146 (= Y164), K150 (= K168), P173 (≠ H191), A174 (≠ S192), V176 (≠ M194)
- binding uridine-5'-diphosphate-glucose: I81 (≠ V83), R84 (≠ T86), S121 (= S123), G123 (≠ N125), Y146 (= Y164), A174 (≠ S192), N175 (≠ S193), A187 (≠ Q205), G188 (= G206), V189 (≠ W207), F193 (= F211), R204 (≠ T228), F206 (≠ H230), N211 (≠ Q235), R213 (= R237), D248 (≠ L274), R271 (= R299)
P27830 dTDP-glucose 4,6-dehydratase 2; EC 4.2.1.46 from Escherichia coli (strain K12) (see 2 papers)
28% identity, 98% coverage: 2:335/342 of query aligns to 3:330/355 of P27830
- FI 12:13 (≠ FL 11:12) binding NAD(+)
- DKLT 33:36 (≠ DNLY 31:34) binding NAD(+)
- DI 59:60 (≠ DV 57:58) binding NAD(+)
- T100 (≠ V98) binding NAD(+)
- D135 (≠ N125) active site, Proton donor
- E136 (≠ K126) active site, Proton acceptor
- Y160 (= Y164) active site, Proton acceptor
- YSASK 160:164 (≠ YGCSK 164:168) binding NAD(+)
- N190 (≠ M194) binding NAD(+)
6bi4C 2.9 angstrom resolution crystal structure of dtdp-glucose 4,6- dehydratase (rfbb) from bacillus anthracis str. Ames in complex with NAD. (see paper)
29% identity, 99% coverage: 1:337/342 of query aligns to 2:301/311 of 6bi4C
- active site: T117 (≠ S124), D118 (≠ N125), E119 (≠ K126), Y142 (= Y164), K146 (= K168)
- binding beta-D-fructofuranose: Q62 (≠ R59), N63 (= N60), G64 (≠ R61), E65 (= E62)
- binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G10), F12 (= F11), I13 (≠ L12), D34 (= D31), A35 (≠ N32), L36 (= L33), T37 (≠ Y34), S39 (≠ V36), G40 (= G37), E60 (≠ D57), I61 (≠ V58), Q62 (≠ R59), F82 (≠ L79), A83 (= A80), A84 (≠ G81), T92 (≠ V98), V115 (≠ S122), T117 (≠ S124), Y142 (= Y164), K146 (= K168), C169 (≠ H191), S170 (= S192)
1bxkB Dtdp-glucose 4,6-dehydratase from e. Coli
28% identity, 98% coverage: 2:335/342 of query aligns to 3:324/344 of 1bxkB
- active site: S125 (= S117), T134 (≠ S124), D135 (≠ N125), E136 (≠ K126), S158 (≠ T162), Y160 (= Y164), S161 (≠ G165), K164 (= K168)
- binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G10), F12 (= F11), I13 (≠ L12), D33 (= D31), K34 (≠ N32), L35 (= L33), T36 (≠ Y34), A38 (≠ V36), G39 (= G37), D59 (= D57), I60 (≠ V58), L81 (= L79), A83 (≠ G81), T100 (≠ V98), I132 (vs. gap), S133 (= S123), T134 (≠ S124), K164 (= K168), C187 (≠ H191)
2p5uA Crystal structure of thermus thermophilus hb8 udp-glucose 4-epimerase complex with NAD
28% identity, 99% coverage: 1:339/342 of query aligns to 1:310/311 of 2p5uA
- active site: T117 (≠ S123), G119 (≠ N125), A120 (≠ K126), Y143 (= Y164), K147 (= K168), H181 (≠ T202), G185 (= G206)
- binding nicotinamide-adenine-dinucleotide: G10 (= G10), F11 (= F11), I12 (≠ L12), D31 (= D31), N32 (= N32), L33 (= L33), A34 (≠ Y34), T35 (≠ V36), G36 (= G37), D51 (= D57), L52 (≠ V58), Q73 (≠ L79), A74 (= A80), A75 (≠ G81), A77 (≠ V83), S116 (= S122), Y143 (= Y164), K147 (= K168), V173 (≠ M194)
6bi4B 2.9 angstrom resolution crystal structure of dtdp-glucose 4,6- dehydratase (rfbb) from bacillus anthracis str. Ames in complex with NAD. (see paper)
29% identity, 99% coverage: 1:337/342 of query aligns to 2:300/310 of 6bi4B
- active site: T117 (≠ S124), D118 (≠ N125), E119 (≠ K126), Y142 (= Y164), K146 (= K168)
- binding alpha-D-glucopyranose: Q62 (≠ R59), N63 (= N60)
- binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G10), F12 (= F11), I13 (≠ L12), D34 (= D31), A35 (≠ N32), L36 (= L33), T37 (≠ Y34), S39 (≠ V36), G40 (= G37), E60 (≠ D57), F82 (≠ L79), A83 (= A80), A84 (≠ G81), T92 (≠ V98), S116 (= S123), T117 (≠ S124), Y142 (= Y164), K146 (= K168), C169 (≠ H191)
2hunA Crystal structure of hypothetical protein ph0414 from pyrococcus horikoshii ot3
29% identity, 98% coverage: 1:335/342 of query aligns to 2:305/329 of 2hunA
- active site: T125 (≠ S124), D126 (≠ N125), E127 (≠ K126), Y149 (= Y164), K153 (= K168)
- binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G10), F12 (= F11), I13 (≠ L12), D34 (= D31), K35 (≠ N32), S40 (≠ G37), D60 (= D57), V61 (= V58), L80 (= L79), A81 (= A80), A82 (≠ G81), S99 (≠ V98), T125 (≠ S124), K153 (= K168), C176 (≠ H191), T177 (≠ S192), N178 (≠ S193), N179 (≠ M194)
6pmhA Structure of epimerase mth375 from the thermophilic pseudomurein- containing methanogen methanothermobacter thermautotrophicus
28% identity, 97% coverage: 4:335/342 of query aligns to 13:316/330 of 6pmhA
- active site: S127 (= S123), C129 (≠ N125), G130 (≠ K126), Y153 (= Y164), K157 (= K168)
- binding adenosine monophosphate: G16 (= G7), G19 (= G10), D40 (= D31), N41 (= N32), S43 (vs. gap), S44 (vs. gap), S45 (vs. gap), D62 (= D57), I63 (≠ V58), A84 (= A80), F87 (≠ V83), R194 (≠ Q205)
- binding uridine-5'-diphosphate: C129 (≠ N125), N182 (≠ S193), N195 (≠ G206), V196 (≠ W207), F200 (= F211), P211 (≠ T228), I212 (= I229), T213 (≠ H230), R220 (= R237), V256 (≠ L276), R279 (= R299)
6pnlA Structure of epimerase mth375 from the thermophilic pseudomurein- containing methanogen methanothermobacter thermautotrophicus
28% identity, 97% coverage: 4:335/342 of query aligns to 19:322/336 of 6pnlA
- active site: S133 (= S123), C135 (≠ N125), G136 (≠ K126), Y159 (= Y164), K163 (= K168)
- binding nicotinamide-adenine-dinucleotide: G22 (= G7), G25 (= G10), C26 (≠ F11), D46 (= D31), N47 (= N32), L48 (= L33), S49 (vs. gap), S50 (vs. gap), S51 (vs. gap), D68 (= D57), I69 (≠ V58), L89 (= L79), A91 (≠ G81), F93 (≠ V83), V108 (= V98), S131 (≠ Y121), S133 (= S123), Y159 (= Y164), K163 (= K168), F186 (≠ H191), N188 (≠ S193), V189 (≠ M194), R200 (≠ Q205)
- binding uridine-5'-diphosphate: N188 (≠ S193), N201 (≠ G206), V202 (≠ W207), F206 (= F211), P217 (≠ T228), I218 (= I229), T219 (≠ H230), R226 (= R237), V262 (≠ L276), R285 (= R299)
1r6dA Crystal structure of desiv double mutant (dtdp-glucose 4,6- dehydratase) from streptomyces venezuelae with NAD and dau bound (see paper)
26% identity, 98% coverage: 1:335/342 of query aligns to 1:310/322 of 1r6dA
- active site: T127 (≠ S124), N128 (= N125), Q129 (≠ K126), Y151 (= Y164), K155 (= K168)
- binding 2'deoxy-thymidine-5'-diphospho-alpha-d-glucose: S87 (≠ V83), H88 (≠ A84), T127 (≠ S124), N128 (= N125), Q129 (≠ K126), Y151 (= Y164), N180 (≠ S193), K190 (≠ Y203), L191 (= L213), P206 (≠ T228), Y208 (≠ H230), R215 (= R237), N250 (≠ L276), R274 (= R299), H277 (≠ D302), Y281 (≠ F306)
- binding nicotinamide-adenine-dinucleotide: G10 (= G10), F11 (= F11), I12 (≠ L12), D37 (= D31), S38 (≠ N32), L39 (= L33), T40 (≠ Y34), A42 (≠ V36), G43 (= G37), D63 (= D57), I64 (≠ V58), F83 (≠ L79), A84 (= A80), A85 (≠ G81), S87 (≠ V83), T102 (≠ V98), V125 (≠ S122), S126 (= S123), Y151 (= Y164), K155 (= K168), N181 (≠ M194)
Query Sequence
>WP_012674632.1 NCBI__GCF_000021545.1:WP_012674632.1
MKYLITGGCGFLGSNLAAEVLKRNEELIIFDNLYRVGSYANLEWLKTQGEFKFIHGDVRN
REDIEVLIKNEKPDVIFHLAGQVAMTTSLENPRLDFEVNAWGTFNLLDVVRKFSPESIVI
YSSSNKVYGDLEWVEYEETETRYIAVDFPNRFPESIPLDFRTPYGCSKGAADQYILDFYK
TYGIRTVVFRHSSMYGGRQFSTYDQGWIGWFCLKAIEIKNSKLKEPFTIHGNGKQVRDVL
HARDMVSLYFKTVENIEKVKGEAFNIGGGIENSLSLIELLDVLEKMLDIKMNYEKLFPRK
SDQKVFVADITKAKKLIGWQPKVSKEEGIKLMVEWIESAYSK
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory