SitesBLAST
Comparing WP_012674805.1 NCBI__GCF_000021545.1:WP_012674805.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 8 hits to proteins with known functional sites (download)
6cyzA Mycobacterial homoserine kinase thrb in complex with amppnp
38% identity, 85% coverage: 8:266/303 of query aligns to 12:268/295 of 6cyzA
P00547 Homoserine kinase; HK; HSK; EC 2.7.1.39 from Escherichia coli (strain K12) (see paper)
30% identity, 85% coverage: 5:262/303 of query aligns to 1:270/310 of P00547
- H139 (= H130) mutation to L: 35-fold decrease in kinase activity.
- H203 (≠ N195) mutation to L: 2-fold decrease in kinase activity but nearly no change in substrates affinity.
- H206 (≠ R198) mutation to Q: 3500-fold decrease in kinase activity.
- R235 (= R227) mutation to H: 250-fold decrease in kinase activity but no change in substrates affinity.; mutation to L: 26200-fold decrease in catalytic efficiency.
1h74B Crystal structure of homoserine kinase complexed with ile (see paper)
29% identity, 86% coverage: 6:266/303 of query aligns to 3:269/296 of 1h74B
- active site: E126 (≠ K122), T179 (= T176)
- binding phosphothiophosphoric acid-adenylate ester: N58 (= N59), V59 (≠ L60), K83 (≠ R91), A87 (≠ S95), G88 (≠ S96), G90 (≠ T98), G92 (≠ I100), S93 (≠ V101), S94 (≠ A102), S97 (vs. gap), S129 (≠ Y125)
- binding isoleucine: A12 (= A15), N13 (= N16), D19 (= D22), H134 (= H130), D136 (= D132), N137 (= N133), R183 (= R180), R231 (= R227), G256 (= G253)
1h74A Crystal structure of homoserine kinase complexed with ile (see paper)
29% identity, 86% coverage: 6:266/303 of query aligns to 3:269/296 of 1h74A
- active site: E126 (≠ K122), T179 (= T176)
- binding adenosine-5'-diphosphate: I51 (≠ F52), N58 (= N59), V59 (≠ L60), K83 (≠ R91), A87 (≠ S95), G88 (≠ S96), S93 (≠ V101), S94 (≠ A102), S97 (vs. gap), T179 (= T176)
- binding isoleucine: N13 (= N16), F18 (= F21), D19 (= D22), H134 (= H130), T179 (= T176), R183 (= R180), R231 (= R227)
1h73A Crystal structure of homoserine kinase complexed with threonine (see paper)
29% identity, 86% coverage: 6:266/303 of query aligns to 3:269/296 of 1h73A
- active site: E126 (≠ K122), T179 (= T176)
- binding phosphoaminophosphonic acid-adenylate ester: I51 (≠ F52), N58 (= N59), V59 (≠ L60), K83 (≠ R91), A87 (≠ S95), G88 (≠ S96), G90 (≠ T98), L91 (≠ A99), G92 (≠ I100), S93 (≠ V101), S94 (≠ A102), S97 (vs. gap)
- binding threonine: N13 (= N16), D19 (= D22), R183 (= R180), R231 (= R227)
1h72C Crystal structure of homoserine kinase complexed with hse (see paper)
29% identity, 86% coverage: 6:266/303 of query aligns to 3:269/296 of 1h72C
- active site: E126 (≠ K122), T179 (= T176)
- binding phosphoaminophosphonic acid-adenylate ester: I51 (≠ F52), N58 (= N59), V59 (≠ L60), K83 (≠ R91), A87 (≠ S95), G88 (≠ S96), G90 (≠ T98), L91 (≠ A99), G92 (≠ I100), S93 (≠ V101), S94 (≠ A102), S97 (vs. gap), S129 (≠ Y125), T179 (= T176), S257 (≠ A254)
- binding l-homoserine: N13 (= N16), F18 (= F21), D19 (= D22), D136 (= D132), N137 (= N133), R183 (= R180), R231 (= R227)
1fwkA Crystal structure of homoserine kinase complexed with adp (see paper)
29% identity, 86% coverage: 6:266/303 of query aligns to 3:269/296 of 1fwkA
Q8L7R2 Homoserine kinase; Protein DOWNY MILDEW RESISTANT 1; EC 2.7.1.39 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
31% identity, 96% coverage: 6:296/303 of query aligns to 54:362/370 of Q8L7R2
- G118 (vs. gap) mutation to R: In dmr1-6; loss of function.
- G180 (≠ A124) mutation to D: In dmr1-5; loss of function.
- G202 (= G141) mutation to R: In dmr1-4; loss of function.
- M241 (≠ A179) mutation to K: In dmr1-3; loss of function.
- A267 (= A205) mutation to V: In dmr1-1; loss of function.
Sites not aligning to the query:
- 46 E→K: In dmr1-2; loss of function.
Query Sequence
>WP_012674805.1 NCBI__GCF_000021545.1:WP_012674805.1
MSKKVVKVRVPATTANLGPGFDTFGLALTLYNEFEVEEEDGVYIESYPKNEFIENPENNL
FIKVIKYLCEAEGKTFHGARLKQTVNIPVARGLGSSATAIVAGILTGFAVHKKPLTDEEF
FKVAYLFEPHPDNLLPAWKGGFITALKTEDKTYYSKMDFPKEIKAVVVIPDFELSTEKAR
LVLPSQVPLKDAIFNLQRSALFIRALEERRYDLLKVVMEDRLHQPYRKNLIPNFDKVIQS
AYENGALGACLSGAGSTILALATENFDKIGLSMVEAFKEVSINAEYKVLEVDTQGATVEI
FEK
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory