SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012674810.1 NCBI__GCF_000021545.1:WP_012674810.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 13 hits to proteins with known functional sites (download)

P9WKJ5 Dihydroxy-acid dehydratase; DAD; EC 4.2.1.9 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
44% identity, 99% coverage: 2:551/557 of query aligns to 20:573/575 of P9WKJ5

query
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P9WKJ5

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C64 (≠ G46) binding [2Fe-2S] cluster
D96 (= D78) binding Mg(2+)
C137 (= C119) binding [2Fe-2S] cluster
D138 (= D120) binding Mg(2+)
K139 (= K121) binding via carbamate group; modified: N6-carboxylysine
C214 (= C192) binding [2Fe-2S] cluster
E465 (= E443) binding Mg(2+)
S491 (= S469) mutation to A: Loss of catalytic activity.

6ovtA Crystal structure of ilvd from mycobacterium tuberculosis (see paper)
44% identity, 99% coverage: 2:551/557 of query aligns to 7:560/562 of 6ovtA

query
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6ovtA

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binding fe2/s2 (inorganic) cluster: C51 (≠ G46), G84 (= G79), C124 (= C119), D125 (= D120), A200 (≠ S191), C201 (= C192)
binding magnesium ion: D83 (= D78), D125 (= D120), K126 (= K121), E452 (= E443)

Q9LIR4 Dihydroxy-acid dehydratase, chloroplastic; AthDHAD; DAD; EC 4.2.1.9 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
41% identity, 99% coverage: 3:555/557 of query aligns to 57:608/608 of Q9LIR4

query
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Q9LIR4

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G

G

H

H

T

F

Q

Q

T

D

G

-

K

K

P

T

I

Y

D

D

L

I

A

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S

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A

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F

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H

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C

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C

A

G

G

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A

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|
C

S

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G

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M

M

F

Y

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N

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M

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G

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N

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V

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L

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H

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L

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A

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M

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|
E

M

M

L

L

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P

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S

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I

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G

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G

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P

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I

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N

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K

K

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D

L

T

L

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D

R

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M

R

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K

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A

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A

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N

S

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W

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L

L

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R

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Y

Y

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K

K

F

N

V

V

T

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S

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A

D

S

K

D

G

G

A

C

I

V

L

-

S

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D

E

E

C100 (≠ G46) binding [2Fe-2S] cluster
C173 (= C119) binding [2Fe-2S] cluster
D174 (= D120) binding Mg(2+)
C245 (= C192) binding [2Fe-2S] cluster
E497 (= E443) binding Mg(2+)

8hs0A The mutant structure of dhad v178w
41% identity, 99% coverage: 3:555/557 of query aligns to 19:570/570 of 8hs0A

query
sites
8hs0A

S

S

D

S

E

R

I

I

K

T

K

E

G

P

I

K

E

S

R

Q

A

G

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G

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D

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L

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K

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P

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I

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G

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Y

W

I

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D

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I

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T

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x
C

H

N

V

M

H

H

L

L

Q

L

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K

F

L

A

S

K

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H

A

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V

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K

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I

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N

A

A

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G

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M

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V

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G

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F

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F

F

N

N

V

T

I

I

G

G

V

V

D

S

D
|
D

G
x
A

I

I

A

S

M

M

G

G

H

T

S

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G

G

M

M

F

C

Y

F

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S

L

L

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Q

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R

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D

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L

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I

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D

D

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G

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N

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L

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C
|
C

D
|
D

K
|
K

I

N

V

M

P

P

G

G

M

T

I

I

M

M

A

A

A

M

A

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R

R

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L

N

N

I

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P

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I

I

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Y

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K

K

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I

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L

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H

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C

P

P

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C

A

G

G

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A

C
|
C

S

G

G

G

M

M

F

Y

T

T

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A

N

N

S

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M

M

N

A

C

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G

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D

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K

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D

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N

A

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M

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V

L

S

D

V

I

M

A

A

M

L

G

G

G

G

S

S

S

T

N
|
N

T

A

V

V

L

L

H
|
H

L

L

L

I

A

A

I

I

A

A

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R

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G

G

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M

M

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I

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L

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G

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A

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-

F

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T

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V

V

V

V

V

I

I

R

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K

G

G

G

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G

G

M

M

R

P

E

E

M

M

L

L

A

T

P

P

T

T

S

S

T

A

I

I

M

M

G

G

M

A

G

G

L

L

G

G

D

K

K

E

V

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A

L

L

I

L

T

T

D

D

G

G

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R

F

F

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G

A

G

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S

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H

G

G

A

F

C

V

I

V

G

G

H

H

V

I

S

C

P

P

E

E

A

A

A

Q

A

E

G

G

G

G

P

P

I

I

G

G

I

L

I

I

Q

K

D

N

G

G

D

D

E

I

I

I

L

T

I

I

D

D

I

I

P

G

N

A

R

A

K

R

I

I

E

D

L

T

L

Q

I

V

P

S

Q

P

E

E

E

E

F

M

E

N

K

D

R

R

M

R

K

K

E

K

F

W

K

T

P

A

K

P

R

A

K

Y

E

K

I

V

P

N

S

R

P

G

W

V

L

L

R

Y

R

K

Y

Y

S

I

K

K

F

N

V

V

T

Q

S

S

A

A

D

S

K

D

G

G

A

C

I

V

L

-

S

T

D

D

E

E

binding fe2/s2 (inorganic) cluster: C62 (≠ G46), D94 (= D78), A95 (≠ G79), C135 (= C119), D136 (= D120), C207 (= C192)
binding magnesium ion: D136 (= D120), K137 (= K121), N285 (= N270), H289 (= H274)

8imuA Dihydroxyacid dehydratase (dhad) mutant-v497f
38% identity, 99% coverage: 3:555/557 of query aligns to 16:523/523 of 8imuA

query
sites
8imuA

S

S

D

S

E

R

I

I

K

T

K

E

G

P

I

K

E

S

R

Q

A

G

P

G

H

S

R

Q

S

A

L

I

L

L

R

H

A

G

C

V

G

G

L

L

K

S

D

D

E

D

D

D

F

L

G

L

K

K

P

P

F

Q

I

I

G

G

V

I

A

S

N

S

S

V

Y

W

I

Y

D

E

I

G

I
x
N

P

T

G
x
C

H

N

V

M

H

H

L

L

Q

L

E

K

F

L

A

S

K

E

H

A

V

V

K

K

Q

E

A

G

I

V

R

E

E

N

A

A

G

G

G

M

V

V

P

G

F

F

E

R

F

F

N

N

V

T

I

I

G

G

V

V

D

S

D

D

G

A

I

I

A

S

M

M

G

G

H

T

S

R

G

G

M

M

F

C

Y

F

S

S

L

L

P

Q

S

S

R

R

E

D

L

L

I

I

A

A

D

D

S

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I

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V

V

V

M

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A

A

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Q

K

W

L

Y

D

D

G

G

L

N

V

I

L

S

I

I

P

P

N

G

C
|
C

D
|
D

K

K

I

N

V

M

P

P

G

G

M

T

I

I

M

M

A

A

A

M

A

G

R

R

V

L

N

N

I

R

P

P

T

G

I

I

L

M

V

V

S

Y

G

G

G

G

P

-

M

-

A

-

A

-

G

-

H

-

T

-

Q

-

T

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G

-

K

-

P

-

I

-

D

-

L

-

A

-

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-

V

-

F

-

E

-

A

-

V

-

G

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S

T

I

I

K

K

K

P

G

G

L

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I

-

K

-

E

-

E

-

E

-

L

-

L

-

D

-

I

-

E

-

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-

H

-

A

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C

-

P

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T

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C

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G

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C

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G

G

M

M

F

Y

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A

A

N

N

S

T

M

M

N

A

C

S

L

A

A

I

E

E

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A

L

M

G

G

I

M

A

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L

L

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S

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I

L

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A

A

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A

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M

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L

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M

A

A

M

L

G

G

G

G

S

S

S

T

N

N

T

A

V

V

L

L

H

H

L

L

L

I

A

A

I

I

A

A

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G

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L

L

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D

L

L

A

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A

S

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Y

Y

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M

M

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D

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A

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G

G

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A

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V

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Q

T

T

L

L

G

A

E

Q

A

N

I

L

K

E

D

N

-

V

-

P

A

S

E

L

I

T

K

E

D

G

Y

Q

N

E

V

I

I

I

R

R

P

P

I

L

D

S

N

N

P

P

Y

I

S

K

E

E

T

T

G

G

G

H

L

I

A

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I

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L

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D

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C

S

V

V

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A

K

K

A

I

A

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A

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V

K

D

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G

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-

I

-

M

L

V

Y

H

F

K

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G

K

P

A

A

V

L

V

V

F

F

D

E

S

G

E

E

E

E

E

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A

M

I

L

E

A

G

A

I

I

T

S

N

A

G

D

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P

V

M

K

S

-

F

E

K

G

G

N

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V

V

V

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V

V

I

I

R

R

Y

G

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G

G

P

P

K

K

G

G

G

G

P

P

G

G

M

M

R

P

E

E

M

M

L

L

A

T

P

P

T

T

S

S

T

A

I

I

M

M

G

G

M

A

G

G

L

L

G

G

D

K

K

E

V

C

S

A

L

L

I

L

T

T

D

D

G

G

R

R

F

F

S

S

G

G

A

G

T

S

R

H

G

G

A

F

C

V

I

F

G

G

H

H

V

I

S

C

P

P

E

E

A

A

A

Q

A

E

G

G

G

G

P

P

I

I

G

G

I

L

I

I

Q

K

D

N

G

G

D

D

E

I

I

I

L

T

I

I

D

D

I

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G

N

A

R

A

K

R

I

I

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D

L

T

L

Q

I

V

P

S

Q

P

E

E

E

E

F

M

E

N

K

D

R

R

M

R

K

K

E

K

F

W

K

T

P

A

K

P

R

A

K

Y

E

K

I

V

P

N

S

R

P

G

W

V

L

L

R

Y

R

K

Y

Y

S

I

K

K

F

N

V

V

T

Q

S

S

A

A

D

S

K

D

G

G

A

C

I

V

L

-

S

T

D

D

E

E

binding fe2/s2 (inorganic) cluster: N57 (≠ I44), C59 (≠ G46), C132 (= C119), D133 (= D120)

B5ZZ34 L-arabinonate dehydratase; ArDHT; D-fuconate dehydratase; Galactonate dehydratase; L-arabonate dehydratase; EC 4.2.1.25; EC 4.2.1.67; EC 4.2.1.6 from Rhizobium leguminosarum bv. trifolii (strain WSM2304) (see 2 papers)
37% identity, 99% coverage: 1:552/557 of query aligns to 11:563/579 of B5ZZ34

query
sites
B5ZZ34

M

L

R

R

S

S

D

Q

E

E

I

W

K

Y

K

G

G

G

I

T

E

S

R

R

-

D

-

V

A

I

P

Y

H

H

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R

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G

L

W

L

L

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A

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C

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G

G

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Y

K

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D

H

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D

D

L

F

F

-

D

G

G

K

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P

P

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V

I

I

G

G

V

I

A

L

N

N

S

T

Y

W

I

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D

D

I

M

I

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P

P

G
x
C

H

N

V

G

H

H

L

L

Q

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F

L

A

A

K

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V

K

K

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G

G

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F

P

P

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L

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N

P

V

V

I

F

G

S

V

A

D

S

D
x
E

G

N

I

-

A

T

M

F

G

R

H

P

S

T

G

A

M

M

F

M

Y

Y

S

-

L

-

P

-

S

-

R

R

E

N

L

L

I

A

A

A

D

L

S

A

V

V

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A

V

I

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A

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K

P

L

M

D

D

G

G

L

C

V

V

L

L

I

L

P

V

N

G

C
|
C

D
|
D

K

K

I

T

V

T

P

P

G

S

M

L

I

L

M

M

A

G

A

A

A

A

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S

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C

N

D

I

L

P

P

T

S

I

I

L

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V

V

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G

G

G

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M

M

A

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G

G

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G

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I

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K

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M

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A

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L

D

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I

A

E

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S

A

H

S

A

M

C

S

P

R

T

S

C

S

G

G

S

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C
|
C

S

N

G

T

M

M

F

G

T

T

A

A

N

S

S

T

M

M

N

A

C

S

L

M

A

A

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A

L

L

G

G

I

M

A

A

L

L

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G

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N

G

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A

I

I

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A

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V

D

D

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R

R

R

I

K

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A

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Q

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D

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M

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Q

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N

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A

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I

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R

L

T

D

N

I

A

A

A

M

I

G

G

G

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S

S

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N

N

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V

L

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H

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L

L

L

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A

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I

A

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L

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M

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M

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V

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N

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K

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D

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N

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x
C

V

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V

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C

G

G

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P

K

K

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G

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P

P

G

G

M

M

R

A

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|
E

M

V

-

G

-

N

L

M

A

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M

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M

K

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G

L

I

G

L

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D

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M

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V

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I

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D

D

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R

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S
|
S

G

G

A

T

T

A

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Y

G

G

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C

V

I

V

G

L

H

H

V

T

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S

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P

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A

A

A

A

A

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G

G

G

G

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P

I

L

G

A

I

V

I

V

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K

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N

G

G

D

D

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M

I

I

L

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I

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D

I

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P

N

N

R

R

K

R

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L

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H

L

L

L

D

I

I

P

S

Q

D

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E

E

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F

L

E

A

K

R

R

R

M

L

K

A

E

E

F

W

K

Q

P

P

K

N

R

H

K

D

E

L

I

P

P

T

S

S

P

G

W

Y

L

A

R

F

R

L

Y

H

S

Q

K

Q

F

H

V

V

T

E

S

G

A

A

D

D

K

T

G

G

A

A

I

D

L

L

C59 (≠ G46) binding [2Fe-2S] cluster; mutation to S: Loss of activity. Does not bind iron-sulfur cluster.
E91 (≠ D78) binding Mg(2+)
C127 (= C119) binding [2Fe-2S] cluster; mutation to S: Loss of activity. Does not bind iron-sulfur cluster.
D128 (= D120) binding Mg(2+)
C200 (= C192) binding [2Fe-2S] cluster; mutation to S: Almost loss of activity. Does not bind iron-sulfur cluster.
C436 (≠ N426) mutation to S: No change in activity. Does not affect binding of iron-sulfur cluster.
C443 (≠ E433) mutation to S: Slight decrease in activity. Does not affect binding of iron-sulfur cluster.
E453 (= E443) binding Mg(2+)
S480 (= S469) mutation to A: Loss of activity.

5j85A Ser480ala mutant of l-arabinonate dehydratase (see paper)
37% identity, 99% coverage: 1:552/557 of query aligns to 8:560/576 of 5j85A

query
sites
5j85A

M

L

R

R

S

S

D

Q

E

E

I

W

K

Y

K

G

G

G

I

T

E

S

R

R

-

D

-

V

A

I

P

Y

H

H

R

R

S

G

L

W

L

L

R

K

A

N

C

Q

G

G

L

Y

K

P

D

H

E

D

D

L

F

F

-

D

G

G

K

R

P

P

F

V

I

I

G

G

V

I

A

L

N

N

S

T

Y

W

I

S

D

D

I

M

I

T

P

P

G
x
C

H

N

V

G

H

H

L

L

Q

R

E

E

F

L

A

A

K

E

H

K

V

V

K

K

Q

A

A

G

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W

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A

G

G

G

G

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P

P

F

L

E

E

F

V

N

P

V

V

I

F

G

S

V

A

D

S

D
x
E

G
x
N

I

-

A

T

M

F

G

R

H

P

S

T

G

A

M

M

F

M

Y

Y

S

-

L

-

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-

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-

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R

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N

L

L

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A

A

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V

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A

V

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Q

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M

D

D

G

G

L

C

V

V

L

L

I

L

P

V

N

G

C
|
C

D
|
D

K
|
K

I

T

V

T

P

P

G

S

M

L

I

L

M

M

A

G

A

A

A

A

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C

N

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I

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P

P

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I

I

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V

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G

G

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M

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M

I

V

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K

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A

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G

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M

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F

L

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I

A

E

E

S

A

H

S

A

M

C

S

P

R

T

S

C

S

G

G

S
x
T

C
|
C

S

N

G

T

M

M

F

G

T
|
T

A

A

N

S

S

T

M

M

N

A

C

S

L

M

A

A

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E

V

A

L

L

G

G

I

M

A

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N

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L

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A

A

M

I

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G

G

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S

S

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N

N

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A

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V

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H

L

L

L

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L

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M

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L

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K

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V

I

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L

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P

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K

P

A

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L

S

T

E

S

T

S

G

G

G

G

L

I

A

V

V

V

L

L

F

R

G

G

N

N

I

L

A

A

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P

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C

A

V

V

V

L

K

K

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A

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A

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H

I

L

M

L

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V

H

H

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K

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G

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V

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F

F

D

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S

D

E

I

E

D

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D

A

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K

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A

G

K

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D

-

D

N

N

G

L

K

D

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I

K

D

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N

N

C

V

I

V

M

V

V

I

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E

C

G

G

P

P

K

K

G

G

G

Y

P

P

G

G

M

M

R

A

E
|
E

M

V

-

G

-

N

L

M

A

G

P

L

T

P

S

P

T

K

I

V

M

L

G

K

M

K

G

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D

K

M

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I

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D

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A

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I

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G

D

D

E

M

I

I

L

E

I

L

D

D

I

V

P

P

N

N

R

R

K

R

I

L

E

H

L

L

L

D

I

I

P

S

Q

D

E

E

E

E

F

L

E

A

K

R

R

R

M

L

K

A

E

E

F

W

K

Q

P

P

K

N

R

H

K

D

E

L

I

P

P

T

S

S

P

G

W

Y

L

A

R

F

R

L

Y

H

S

Q

K

Q

F

H

V

V

T

E

S

G

A

A

D

D

K

T

G

G

A

A

I

D

L

L

binding fe2/s2 (inorganic) cluster: C56 (≠ G46), N89 (≠ G79), C124 (= C119), D125 (= D120), T196 (≠ S191), C197 (= C192)
binding magnesium ion: E88 (≠ D78), D125 (= D120), K126 (= K121), T202 (= T197), E450 (= E443)

8ej0A Crystal structure of fe-s cluster-dependent dehydratase from paralcaligenes ureilyticus in complex with mg
36% identity, 97% coverage: 15:552/557 of query aligns to 18:552/568 of 8ej0A

query
sites
8ej0A

H

Y

R

R

S

S

L

W

L

M

R

K

A

N

C

R

G

G

L

I

K

P

D

H

E

D

D

Q

F

F

-

D

G

G

K

R

P

P

F

V

I

I

G

G

V

I

A

C

N

N

S

T

Y

F

I

S

D

E

I

L

I

T

P

P

G
x
C

H

N

V

S

H

H

L

F

Q

R

E

T

F

L

A

A

K

E

H

Q

V

V

K

K

Q

I

A

G

I

V

R

W

E

E

A

S

G

G

G

G

V

F

P

P

F

L

E

E

F

F

N

P

V

V

I

M

G

S

V

L

D

G

D
x
E

G

T

I

M

A

L

M

R

G

P

H

T

S

A

G

M

M

L

F

F

Y

R

S

N

L

L

P

A

S

S

R

M

E

D

L

-

I

-

A

-

D

-

S

-

V

V

E

E

T

E

V

S

V

I

Q

R

A

G

H

N

K

P

L

L

D

D

G

G

L

V

V

V

L

L

I

L

P

M

N

G

C
|
C

D
|
D

K
|
K

I

T

V

T

P

P

G

S

M

L

I

M

M

M

A

G

A

A

A

A

R

S

V

C

N

D

I

L

P

P

T

T

I

I

L

G

V

V

S

S

G

G

G

G

P

P

M

M

A

L

A

S

G

G

H

K

T

F

Q

R

T

-

G

G

K

R

P

E

I

L

D

G

L

S

A

G

T

T

-

D

V

V

F
x
W

E

K

A

M

V

S

G

E

S

E

I

V

K

R

K

A

G

G

L

Q

I

M

K

S

E

Q

E

E

E

E

L

F

L

F

D

E

I

A

E

E

S

S

H

C

A

M

C

H

P

R

T

S

C

H

G

G

S

H

C
|
C

S

M

G

T

M

M

F

G

T
|
T

A

A

N

S

S

T

M

M

N

A

C

S

L

M

A

V

E

E

V

A

L

L

G

G

I

M

A

S

L

L

P

P

G

G

N

N

G

A

S

A

I

I

L

P

A

A

V

V

D

D

P

A

R

R

R

R

I

N

E

L

L

L

A

A

K

R

Q

A

A

S

G

G

R

R

Q

R

I

I

V

V

D

Q

L

M

V

V

K

K

A

D

N

D

I

L

K

V

F

M

R

S

D

K

I

I

V

L

N

T

Y

R

Q

Q

T

A

I

F

E

E

N

N

A

A

F

I

T

R

L

V

D

N

I

A

A

A

M

I

G

G

G

G

S

S

S

T

N

N

T

A

V

V

L

I

H

H

L

L

L

L

A

A

I

I

A

A

H

G

E

R

A

I

G

G

I

V

E

D

F

L

P

T

M

L

E

A

K

D

I

W

D

D

E

A

I

L

S

G

R

H

R

K

T

L

P

P

T

C

L

L

C

V

K

D

L

L

A

Q

P

P

A

S

S

G

Q

T

Y

H

H

L

M

M

E

E

D

D

L

F

D

Y

K

Y

A

A

G

G

G

G

I

V

Y

P

A

A

I

V

L

I

K

R

E

E

L

L

S

G

K

-

K

-

N

D

L

V

L

I

H

A

L

R

D

D

R

A

P

L

T

T

V

V

L

N

M

G

K

Q

T

T

L

L

G

W

E

D

A

N

I

C

K

K

D

D

A

A

E

P

I

N

K

W

D

N

Y

R

N

E

V

V

I

I

R

H

P

A

I

F

D

N

N

E

P

P

Y

F

S

K

E

T

T

E

G

A

G

G

L

I

A

A

V

V

L

L

F

R

G

G

N

N

I

L

A

C

P

P

N

D

G

G

C

A

V

V

V

I

K

K

A

P

A

S

A

A

V

A

D

T

P

P

K

A

I

L

M

L

V

K

H

H

K

K

G

G

K

R

A

A

V

V

V

V

F

F

D

E

S

N

E

S

E

E

E

H

A

M

I

H

E

E

G

R

I

M

-

D

-

D

T

E

N

N

G

L

K

D

V

V

K

D

E

E

G

N

N

C

V

V

V

L

V

V

I

L

R

K

Y

N

E

C

G

G

P

P

K

R

G

G

G

Y

P

P

G

G

M

M

R

A

E
|
E

M

A

-

G

-

N

-

M

-

P

L

L

A

P

P

P

T

-

S

-

T

K

I

I

M

L

G

R

M

K

G

G

L

I

G

T

D

D

K

M

V

V

S

R

L

-

I

V

T

S

D

D

G

A

R

R

F

M

S
|
S

G
|
G

A

T

T

A

R

Y

G

G

A

T

C

V

I

V

G

L

H

H

V

V

S

A

P

P

E

E

A

A

A

A

A

A

G

G

G

G

P

P

I

L

G

A

I

L

I

V

Q

Q

D

D

G

G

D

D

E

I

I

I

L

E

I

L

D

D

I

V

P

A

N

A

R

R

K

K

I

L

E

H

L

L

L

H

I

V

P

S

Q

D

E

E

E

E

F

L

E

A

K

R

R

R

M

R

K

E

E

A

F

W

K

Q

P

A

K

P

R

P

K

A

E

P

I

M

P

A

S

R

P

G

W

W

L

V

R

K

R

L

Y

Y

S

V

K

E

F

H

V

V

T

Q

S

Q

A

A

D

N

K

L

G

G

A

A

I

D

L

L

binding bicarbonate ion: D119 (= D120), W161 (≠ F162), T196 (= T197), E442 (= E443), S469 (= S469), S469 (= S469), G470 (= G470)
binding fe2/s2 (inorganic) cluster: C50 (≠ G46), E82 (≠ D78), C118 (= C119), D119 (= D120), C191 (= C192)
binding magnesium ion: E82 (≠ D78), D119 (= D120), K120 (= K121), E442 (= E443)

8epzA Crystal structure of fe-s cluster-dependent dehydratase from paralcaligenes ureilyticus in complex with mn
36% identity, 97% coverage: 15:552/557 of query aligns to 19:553/569 of 8epzA

query
sites
8epzA

H

Y

R

R

S

S

L

W

L

M

R

K

A

N

C

R

G

G

L

I

K

P

D

H

E

D

D

Q

F

F

-

D

G

G

K

R

P

P

F

V

I

I

G

G

V

I

A

C

N

N

S

T

Y

F

I

S

D

E

I

L

I

T

P

P

G
x
C

H

N

V

S

H

H

L

F

Q

R

E

T

F

L

A

A

K

E

H

Q

V

V

K

K

Q

I

A

G

I

V

R

W

E

E

A

S

G

G

G

G

V

F

P

P

F

L

E

E

F

F

N

P

V

V

I

M

G

S

V

L

D

G

D
x
E

G

T

I

M

A

L

M

R

G

P

H

T

S

A

G

M

M

L

F

F

Y

R

S

N

L

L

P

A

S

S

R

M

E

D

L

-

I

-

A

-

D

-

S

-

V

V

E

E

T

E

V

S

V

I

Q

R

A

G

H

N

K

P

L

L

D

D

G

G

L

V

V

V

L

L

I

L

P

M

N

G

C
|
C

D
|
D

K
|
K

I

T

V

T

P

P

G

S

M

L

I

M

M

M

A

G

A

A

A

A

R

S

V

C

N

D

I

L

P

P

T

T

I

I

L

G

V

V

S

S

G

G

G

G

P

P

M

M

A

L

A

S

G

G

H

K

T

F

Q

R

T

-

G

G

K

R

P

E

I

L

D

G

L

S

A

G

T

T

-

D

V

V

F

W

E

K

A

M

V

S

G

E

S

E

I

V

K

R

K

A

G

G

L

Q

I

M

K

S

E

Q

E

E

E

E

L

F

L

F

D

E

I

A

E

E

S

S

H

C

A

M

C

H

P

R

T

S

C

H

G

G

S

H

C
|
C

S

M

G

T

M

M

F

G

T

T

A

A

N

S

S

T

M

M

N

A

C

S

L

M

A

V

E

E

V

A

L

L

G

G

I

M

A

S

L

L

P

P

G

G

N

N

G

A

S

A

I

I

L

P

A

A

V

V

D

D

P

A

R

R

R

R

I

N

E

L

L

L

A

A

K

R

Q

A

A

S

G

G

R

R

Q

R

I

I

V

V

D

Q

L

M

V

V

K

K

A

D

N

D

I

L

K

V

F

M

R

S

D

K

I

I

V

L

N

T

Y

R

Q

Q

T

A

I

F

E

E

N

N

A

A

F

I

T

R

L

V

D

N

I

A

A

A

M

I

G

G

G

G

S

S

S

T

N

N

T

A

V

V

L

I

H

H

L

L

L

L

A

A

I

I

A

A

H

G

E

R

A

I

G

G

I

V

E

D

F

L

P

T

M

L

E

A

K

D

I

W

D

D

E

A

I

L

S

G

R

H

R

K

T

L

P

P

T

C

L

L

C

V

K

D

L

L

A

Q

P

P

A

S

S

G

Q

T

Y

H

H

L

M

M

E

E

D

D

L

F

D

Y

K

Y

A

A

G

G

G

G

I

V

Y

P

A

A

I

V

L

I

K

R

E

E

L

L

S

G

K

-

K

-

N

D

L

V

L

I

H

A

L

R

D

D

R

A

P

L

T

T

V

V

L

N

M

G

K

Q

T

T

L

L

G

W

E

D

A

N

I

C

K

K

D

D

A

A

E

P

I

N

K

W

D

N

Y

R

N

E

V

V

I

I

R

H

P

A

I

F

D

N

N

E

P

P

Y

F

S

K

E

T

T

E

G

A

G

G

L

I

A

A

V

V

L

L

F

R

G

G

N

N

I

L

A

C

P

P

N

D

G

G

C

A

V

V

V

I

K

K

A

P

A

S

A

A

V

A

D

T

P

P

K

A

I

L

M

L

V

K

H

H

K

K

G

G

K

R

A

A

V

V

V

V

F

F

D

E

S

N

E

S

E

E

E

H

A

M

I

H

E

E

G

R

I

M

-

D

-

D

T

E

N

N

G

L

K

D

V

V

K

D

E

E

G

N

N

C

V

V

V

L

V

V

I

L

R

K

Y

N

E

C

G

G

P

P

K

R

G

G

G

Y

P

P

G

G

M

M

R

A

E
|
E

M

A

-

G

-

N

-

M

-

P

L

L

A

P

P

P

T

-

S

-

T

K

I

I

M

L

G

R

M

K

G

G

L

I

G

T

D

D

K

M

V

V

S

R

L

-

I

V

T

S

D

D

G

A

R

R

F

M

S

S

G

G

A

T

T

A

R

Y

G

G

A

T

C

V

I

V

G

L

H

H

V

V

S

A

P

P

E

E

A

A

A

A

A

A

G

G

G

G

P

P

I

L

G

A

I

L

I

V

Q

Q

D

D

G

G

D

D

E

I

I

I

L

E

I

L

D

D

I

V

P

A

N

A

R

R

K

K

I

L

E

H

L

L

L

H

I

V

P

S

Q

D

E

E

E

E

F

L

E

A

K

R

R

R

M

R

K

E

E

A

F

W

K

Q

P

A

K

P

R

P

K

A

E

P

I

M

P

A

S

R

P

G

W

W

L

V

R

K

R

L

Y

Y

S

V

K

E

F

H

V

V

T

Q

S

Q

A

A

D

N

K

L

G

G

A

A

I

D

L

L

binding fe2/s2 (inorganic) cluster: C51 (≠ G46), C119 (= C119), D120 (= D120), C192 (= C192)
binding manganese (ii) ion: E83 (≠ D78), D120 (= D120), K121 (= K121), E443 (= E443)

Q1JUQ1 L-arabonate dehydratase; L-arabinonate dehydratase; EC 4.2.1.25 from Azospirillum brasilense (see paper)
37% identity, 92% coverage: 15:526/557 of query aligns to 24:535/583 of Q1JUQ1

query
sites
Q1JUQ1

H

Y

R

R

S

S

L

W

L

M

R

K

A

N

C

Q

G

G

L

I

K

P

D

D

E

H

D

E

F

F

-

D

G

G

K

R

P

P

F

I

I

I

G

G

V

I

A
x
C

N

N

S

T

Y

W

I

S

D

E

I

L

I

T

P

P

G
x
C

H

N

V

A

H

H

L

F

Q

R

E

K

F

L

A

A

K

E

H

H

V

V

K

K

Q

R

A

G

I

I

R

S

E

E

A

A

G

G

G

G

V

F

P

P

F

V

E

E

F

F

N

P

V

V

I

F

G

-

V

-

D

-

D

-

G

-

I

-

A

S

M

N

G

G

H

E

S

S

G

N

M

L

F

R

Y

P

S

S

-

A

L

M

P

L

S

T

R

R

E

N

L

L

I

A

A

S

D

M

S

D

V

V

E

E

T

E

V

A

V

I

Q

R

A

G

H

N

K

P

L

I

D

D

G

A

L

V

V

V

L

L

I

L

P

A

N

G

C
|
C

D

D

K

K

I

T

V

T

P

P

G

A

M

L

I

L

M

M

A

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A

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C

N

D

I

V

P

P

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A

I

I

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V

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S

G

G

G

G

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M

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G

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G

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|
C

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M

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C47 (≠ A37) mutation to A: 2-fold reduction in activity and still binds a 4Fe-4S cluster.
C56 (≠ G46) mutation to A: Loss of activity but seems to be able to bind a Fe-S cluster.
C124 (= C119) binding [4Fe-4S] cluster; mutation to A: Loss of activity and no 4Fe-4S cluster binding.
C197 (= C192) binding [4Fe-4S] cluster; mutation to A: Loss of activity and no 4Fe-4S cluster binding.
C441 (≠ E433) mutation to A: Increase in activity and still binds a 4Fe-4S cluster.

7m3kA Crystal structure of galactonate dehydratase from brucella melitensis biovar abortus 2308
34% identity, 96% coverage: 23:554/557 of query aligns to 28:573/587 of 7m3kA

query
sites
7m3kA

G

G

L

L

K

S

D

Q

E

E

D

E

F

L

-

Q

-

S

G

G

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I

I

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G

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A

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D

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L

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P

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x
C

H

N

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A

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K

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A

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V

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V

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C
|
C

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D

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K

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P

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C

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M

M

A

A

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N

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G

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K

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W

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N

binding zinc ion: C53 (≠ G46), C121 (= C119)

Q9A9Z2 D-xylonate dehydratase; XyDHT; Gluconate dehydratase; EC 4.2.1.82; EC 4.2.1.39 from Caulobacter vibrioides (strain ATCC 19089 / CIP 103742 / CB 15) (Caulobacter crescentus) (see 2 papers)
33% identity, 94% coverage: 30:552/557 of query aligns to 48:577/595 of Q9A9Z2

query
sites
Q9A9Z2

G

G

K

K

P

P

F

I

I

I

G

G

V

I

A

A

N

Q

S

T

Y

G

I

S

D

D

I

I

I

S

P

P

-
x
C

G

N

H

R

V

I

H

H

L

L

Q

-

E

D

F

L

A

V

K

Q

H

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V

V

K

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A

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I

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A

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G

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G

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P

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M

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E

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V

I

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G

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V

I

D

F

D
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E

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N

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C

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L

L

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D

D

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A

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V

V

L

L

I

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P

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G

C
|
C

D
|
D

K

K

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T

V

T

P

P

G

A

M

G

I

I

M

M

A

A

A

A

A

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V

V

N

N

I

I

P

P

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V

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S

G

G

G

G

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M

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A

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A

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G

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C
|
C

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M

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M

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N

E

P

P

Y

L

S

A

E

E

T

K

G

A

G

G

L

F

A

L

V

V

L

L

F

K

G

G

N

N

I

L

A

F

P

-

N

D

G

F

C

A

V

I

V

M

K

K

A

S

A

S

A

V

V

I

D

G

P

E

K

E

I

F

M

R

-

K

-

R

-

Y

-

L

-

S

-

Q

-

P

-

G

-

Q

-

E

-

G

V

V

H

F

K

E

G

A

K

R

A

A

V

I

V

V

F

F

D

D

S

G

E

S

E

D

E

D

A

Y

I

H

E

K

G

R

I

I

T

N

N

D

G

P

-

A

-

L

K

E

V

I

K

D

E

E

G

R

N
x
C

V

I

V

L

V

V

I

I

R

R

Y

G

E

A

G

G

P

P

K

I

G

G

G

W

P

P

G

G

M

S

R

A

E
|
E

M

V

-

V

-

N

L

M

A

Q

P

P

T

P

S

D

T

H

I

L

M

L

G

K

M

K

G

G

L

I

G

-

D

-

K

-

V

M

S

S

L

L

I

P

T

T

-

L

-

G

D

D

G

G

R

R

F

Q

S

S

G

G

A

T

T

A

R

D

G

S

A

P

C

S

I

I

G

L

H

N

V

A

S

S

P

P

E

E

A

S

A

A

A

I

G

G

G

G

P

G

I

L

G

S

I

W

I

L

Q

R

D

T

G

G

D

D

E

T

I

I

L

R

I

I

D

D

I

L

P

N

N

T

R

G

K

R

I

C

E

D

L

A

L

L

I

V

P

D

Q

E

E

A

E

T

F

I

E

A

K

A

R

R

M

K

K

Q

E

D

F

G

K

I

P

P

K

A

R

V

K

P

E

A

I

T

P

M

S

T

P

P

W

W

L

Q

R

E

R

I

Y

Y

S

R

K

A

F

H

V

A

T

S

S

Q

A

L

D

D

K

T

G

G

A

G

I

V

L

L

C64 (vs. gap) binding [2Fe-2S] cluster; mutation to S: Strong decrease in activity. Does not bind iron-sulfur cluster.
E96 (≠ D78) binding Mg(2+)
C132 (= C119) binding [2Fe-2S] cluster; mutation to S: Almost loss of activity. Does not bind iron-sulfur cluster.
D133 (= D120) binding Mg(2+)
C205 (= C192) binding [2Fe-2S] cluster; mutation to S: Strong decrease in activity. Does not bind iron-sulfur cluster.
C450 (≠ N426) mutation to S: Slight decrease in activity. Does not affect binding of iron-sulfur cluster.
E467 (= E443) binding Mg(2+)

5oynA Crystal structure of d-xylonate dehydratase in holo-form (see paper)
33% identity, 94% coverage: 30:552/557 of query aligns to 42:571/589 of 5oynA

query
sites
5oynA

G

G

K

K

P

P

F

I

I

I

G

G

V

I

A

A

N

Q

S

T

Y

G

I

S

D

D

I

I

I

S

P

P

-
x
C

G

N

H

R

V

I

H

H

L

L

Q

-

E

D

F

L

A

V

K

Q

H

R

V

V

K

R

Q

D

A

G

I

I

R

R

E

D

A

A

G

G

G

G

V

I

P

P

F

M

E

E

F

F

N

P

V

V

I

H

G

P

V

I

D

F

D
x
E

G
x
N

I

C

A

R

M

R

G

P

H

T

S

A

G

A

M

L

F

D

Y

R

S

N

L

L

P

S

S

Y

R

L

E

G

L

L

I

-

A

-

D

-

S

-

V

V

E

E

T

T

V

L

V

-

Q

H

A

G

H

Y

K

P

L

I

D

D

G

A

L

V

V

V

L

L

I

T

P

T

N

G

C
|
C

D
|
D

K
|
K

I

T

V

T

P

P

G

A

M

G

I

I

M

M

A

A

A

A

A

T

R

T

V

V

N

N

I

I

P

P

T

A

I

I

L

V

V

L

S

S

G

G

G

G

P

P

M

M

A

L

A

D

G

G

H

W

T

H

Q

E

T

N

G

E

K

L

P

V

I

G

D

S

L

G

A

T

T

V

V

I

F

W

E

R

A

S

V

R

G

R

S

K

I

L

K

A

K

A

G

G

L

E

I

I

K

T

E

E

E

E

E

E

L

F

L

I

D

D

I

R

E

A

S

A

H

S

A

S

C

A

P

P

T

S

C

A

G

G

S

H

C
|
C

S

N

G

T

M

M

F

G

T

T

A

A

N

S

S

T

M

M

N

N

C

A

L

V

A

A

E

E

V

A

L

L

G

G

I

L

A

S

L

L

P

T

G

G

N

C

G

A

S

A

I

I

L

P

A

A

V

P

D

Y

P

R

R

E

R

R

I

G

E

Q

L

M

A

A

K

Y

Q

K

A

T

G

G

R

Q

Q

R

I

I

V

V

D

D

L

L

V

A

K

Y

A

D

N

D

I

V

K

K

F

P

R

L

D

D

I

I

V

L

N

T

Y

K

Q

Q

T

A

I

F

E

E

N

N

A

A

F

I

T

A

L

L

D

V

I

A

A

A

M

A

G

G

G

G

S

S

S

T

N

N

T

A

V

Q

L

P

H

H

L

I

L

V

A

A

I

M

A

A

H

R

E

H

A

A

G

G

I

V

E

E

F

I

P

T

M

A

E

D

K

D

I

W

D

-

E

R

I

A

S

A

R

Y

R

D

T

I

P

P

T

L

L

I

C

V

K

N

L

M

A

Q

P

P

A

A

S

G

Q

K

Y

Y

H

L

M

G

E

E

D

R

L

F

D

H

K

R

A

A

G

G

G

G

I

A

Y

P

A

A

I

V

L

L

K

W

E

E

L

L

S

L

K

Q

K

Q

N

G

L

R

L

L

H

H

L

G

D

D

R

V

P

L

T

T

V

V

L

T

M

G

K

K

T

T

L

M

G

S

E

E

A

N

I

L

K

Q

D

G

A

R

E

E

I

T

K

S

D

D

Y

R

N

E

V

V

I

I

R

F

P

P

I

Y

D

H

N

E

P

P

Y

L

S

A

E

E

T

K

G

A

G

G

L

F

A

L

V

V

L

L

F

K

G

G

N

N

I

L

A

F

P

-

N

D

G

F

C

A

V

I

V

M

K

K

A

S

A

S

A

V

V

I

D

G

P

E

K

E

I

F

M

R

-

K

-

R

-

Y

-

L

-

S

-

Q

-

P

-

G

-

Q

-

E

-

G

V

V

H

F

K

E

G

A

K

R

A

A

V

I

V

V

F

F

D

D

S

G

E

S

E

D

E

D

A

Y

I

H

E

K

G

R

I

I

T

N

N

D

G

P

-

A

-

L

K

E

V

I

K

D

E

E

G

R

N

C

V

I

V

L

V

V

I

I

R

R

Y

G

E

A

G

G

P

P

K

I

G

G

G

W

P

P

G

G

M

S

R

A

E
|
E

M

V

-

V

-

N

L

M

A

Q

P

P

T

P

S

D

T

H

I

L

M

L

G

K

M

K

G

G

L

I

G

-

D

-

K

-

V

M

S

S

L

L

I

P

T

T

-

L

-

G

D

D

G

G

R

R

F

Q

S

S

G

G

A

T

T

A

R

D

G

S

A

P

C

S

I

I

G

L

H

N

V

A

S

S

P

P

E

E

A

S

A

A

A

I

G

G

G

G

P

G

I

L

G

S

I

W

I

L

Q

R

D

T

G

G

D

D

E

T

I

I

L

R

I

I

D

D

I

L

P

N

N

T

R

G

K

R

I

C

E

D

L

A

L

L

I

V

P

D

Q

E

E

A

E

T

F

I

E

A

K

A

R

R

M

K

K

Q

E

D

F

G

K

I

P

P

K

A

R

V

K

P

E

A

I

T

P

M

S

T

P

P

W

W

L

Q

R

E

R

I

Y

Y

S

R

K

A

F

H

V

A

T

S

S

Q

A

L

D

D

K

T

G

G

A

G

I

V

L

L

binding fe2/s2 (inorganic) cluster: C58 (vs. gap), N91 (≠ G79), C126 (= C119), D127 (= D120), C199 (= C192)
binding magnesium ion: E90 (≠ D78), D127 (= D120), K128 (= K121), E461 (= E443)

Query Sequence

>WP_012674810.1 NCBI__GCF_000021545.1:WP_012674810.1
MRSDEIKKGIERAPHRSLLRACGLKDEDFGKPFIGVANSYIDIIPGHVHLQEFAKHVKQA
IREAGGVPFEFNVIGVDDGIAMGHSGMFYSLPSRELIADSVETVVQAHKLDGLVLIPNCD
KIVPGMIMAAARVNIPTILVSGGPMAAGHTQTGKPIDLATVFEAVGSIKKGLIKEEELLD
IESHACPTCGSCSGMFTANSMNCLAEVLGIALPGNGSILAVDPRRIELAKQAGRQIVDLV
KANIKFRDIVNYQTIENAFTLDIAMGGSSNTVLHLLAIAHEAGIEFPMEKIDEISRRTPT
LCKLAPASQYHMEDLDKAGGIYAILKELSKKNLLHLDRPTVLMKTLGEAIKDAEIKDYNV
IRPIDNPYSETGGLAVLFGNIAPNGCVVKAAAVDPKIMVHKGKAVVFDSEEEAIEGITNG
KVKEGNVVVIRYEGPKGGPGMREMLAPTSTIMGMGLGDKVSLITDGRFSGATRGACIGHV
SPEAAAGGPIGIIQDGDEILIDIPNRKIELLIPQEEFEKRMKEFKPKRKEIPSPWLRRYS
KFVTSADKGAILSDECS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory