SitesBLAST
Comparing WP_012675267.1 NCBI__GCF_000021565.1:WP_012675267.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
5temA Structure of 4-hydroxy-tetrahydrodipicolinate reductase from vibrio vulnificus with 2,6 pyridine dicarboxylic and nadh (see paper)
44% identity, 100% coverage: 1:268/269 of query aligns to 1:266/266 of 5temA
- active site: H155 (= H159), K159 (= K163)
- binding nicotinamide-adenine-dinucleotide: G8 (= G8), G11 (= G11), R12 (= R12), M13 (= M13), E34 (= E34), R35 (≠ S35), F75 (= F75), T76 (≠ A76), S80 (≠ E80), G98 (= G101), T100 (= T103), P123 (= P126), N124 (= N127), Y125 (≠ M128), F239 (= F241)
- binding pyridine-2,6-dicarboxylic acid: T100 (= T103), P123 (= P126), H156 (= H160), K159 (= K163), S164 (= S168), G165 (= G169), T166 (= T170)
5tejB Structure of 4-hydroxy-tetrahydrodipicolinate reductase from vibrio vulnificus with 2,5 furan dicarboxylic and nadh (see paper)
44% identity, 100% coverage: 1:269/269 of query aligns to 1:267/269 of 5tejB
- active site: H155 (= H159), K159 (= K163)
- binding 2,5 Furan Dicarboxylic Acid: T100 (= T103), H156 (= H160), K159 (= K163), S164 (= S168), G165 (= G169), T166 (= T170)
- binding nicotinamide-adenine-dinucleotide: G8 (= G8), G11 (= G11), R12 (= R12), M13 (= M13), E34 (= E34), R35 (≠ S35), F75 (= F75), T76 (≠ A76), S80 (≠ E80), G98 (= G101), T100 (= T103), P123 (= P126), N124 (= N127), Y125 (≠ M128), F239 (= F241)
5tejA Structure of 4-hydroxy-tetrahydrodipicolinate reductase from vibrio vulnificus with 2,5 furan dicarboxylic and nadh (see paper)
44% identity, 100% coverage: 1:269/269 of query aligns to 1:267/269 of 5tejA
- active site: H155 (= H159), K159 (= K163)
- binding nicotinamide-adenine-dinucleotide: G8 (= G8), G11 (= G11), R12 (= R12), M13 (= M13), E34 (= E34), R35 (≠ S35), F75 (= F75), T76 (≠ A76), S80 (≠ E80), G98 (= G101), T100 (= T103), P123 (= P126)
4ywjA Crystal structure of 4-hydroxy-tetrahydrodipicolinate reductase (htpa reductase) from pseudomonas aeruginosa
42% identity, 100% coverage: 1:269/269 of query aligns to 1:268/268 of 4ywjA
- active site: H156 (= H159), K160 (= K163)
- binding nicotinamide-adenine-dinucleotide: G11 (= G11), R12 (= R12), M13 (= M13), D35 (≠ E34), R36 (≠ S35), F76 (= F75), T77 (= T79), V81 (≠ L83), G99 (= G101), T101 (= T103), A124 (≠ P126), N125 (= N127), F126 (≠ M128), R237 (= R238), F240 (= F241)
1drvA Escherichia coli dhpr/acnadh complex (see paper)
40% identity, 100% coverage: 2:269/269 of query aligns to 3:268/270 of 1drvA
- active site: H156 (= H159), K160 (= K163)
- binding 3-acetylpyridine adenine dinucleotide: G9 (= G8), G12 (= G11), R13 (= R12), M14 (= M13), E35 (= E34), F76 (= F75), T77 (≠ A76), R78 (≠ S78), G81 (≠ A81), G99 (= G101), A124 (≠ P126), F126 (≠ M128), R237 (= R238)
1druA Escherichia coli dhpr/nadh complex (see paper)
40% identity, 100% coverage: 2:269/269 of query aligns to 3:268/270 of 1druA
- active site: H156 (= H159), K160 (= K163)
- binding nicotinamide-adenine-dinucleotide: G9 (= G8), G12 (= G11), R13 (= R12), M14 (= M13), E35 (= E34), R36 (≠ S35), F76 (= F75), T77 (≠ A76), R78 (≠ S78), G81 (≠ A81), G99 (= G101), T100 (= T102), T101 (= T103), A124 (≠ P126), N125 (= N127), F126 (≠ M128), F240 (= F241)
1arzA Escherichia coli dihydrodipicolinate reductase in complex with nadh and 2,6 pyridine dicarboxylate (see paper)
40% identity, 100% coverage: 2:269/269 of query aligns to 3:268/270 of 1arzA
1drwA Escherichia coli dhpr/nhdh complex (see paper)
40% identity, 100% coverage: 2:269/269 of query aligns to 5:270/272 of 1drwA
- active site: H158 (= H159), K162 (= K163)
- binding nicotinamide purin-6-ol-dinucleotide: G11 (= G8), G14 (= G11), R15 (= R12), M16 (= M13), E37 (= E34), R38 (≠ S35), F78 (= F75), T79 (≠ A76), R80 (≠ S78), G101 (= G101), T102 (= T102), T103 (= T103), A126 (≠ P126), N127 (= N127), F128 (≠ M128), F242 (= F241)
1dihA Three-dimensional structure of e. Coli dihydrodipicolinate reductase (see paper)
40% identity, 100% coverage: 2:269/269 of query aligns to 5:270/272 of 1dihA
- active site: H158 (= H159), K162 (= K163)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (= G8), G14 (= G11), R15 (= R12), M16 (= M13), R38 (≠ S35), F78 (= F75), T79 (≠ A76), R80 (≠ S78), G83 (≠ A81), G101 (= G101), T103 (= T103), N127 (= N127), F128 (≠ M128), R239 (= R238), F242 (= F241)
1arzB Escherichia coli dihydrodipicolinate reductase in complex with nadh and 2,6 pyridine dicarboxylate (see paper)
40% identity, 100% coverage: 2:269/269 of query aligns to 2:267/269 of 1arzB
- active site: H155 (= H159), K159 (= K163)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G8 (= G8), G10 (≠ M10), G11 (= G11), R12 (= R12), M13 (= M13), E34 (= E34), F75 (= F75), T76 (≠ A76), R77 (≠ S78), G80 (≠ A81), H84 (= H85), G98 (= G101), T100 (= T103), A123 (≠ P126), N124 (= N127), F125 (≠ M128), F239 (= F241)
- binding pyridine-2,6-dicarboxylic acid: T100 (= T103), H156 (= H160), K159 (= K163), S164 (= S168), G165 (= G169), T166 (= T170), F239 (= F241)
P04036 4-hydroxy-tetrahydrodipicolinate reductase; HTPA reductase; EC 1.17.1.8 from Escherichia coli (strain K12) (see 3 papers)
40% identity, 100% coverage: 2:269/269 of query aligns to 6:271/273 of P04036
- G12 (= G8) binding NADP(+)
- GRM 15:17 (= GRM 11:13) binding NAD(+)
- RM 16:17 (= RM 12:13) binding NADP(+)
- E38 (= E34) binding NAD(+)
- R39 (≠ S35) binding NADP(+)
- T-R 80:81 (≠ AGS 76:78) binding NAD(+)
- GTT 102:104 (= GTT 101:103) binding NAD(+); binding NADP(+)
- AANF 126:129 (≠ APNM 125:128) binding NAD(+)
- F129 (≠ M128) binding NADP(+)
- H159 (= H159) mutation H->A,Q: 135 to 200-fold reduction in catalytic activity.
- K163 (= K163) binding NAD(+); mutation K->A,C,Q: 625 to 830-fold reduction in catalytic activity.
- R240 (= R238) binding NADP(+)
- F243 (= F241) binding NAD(+)
3ijpB Crystal structure of dihydrodipicolinate reductase from bartonella henselae at 2.0a resolution (see paper)
38% identity, 100% coverage: 2:269/269 of query aligns to 2:267/267 of 3ijpB
3ijpA Crystal structure of dihydrodipicolinate reductase from bartonella henselae at 2.0a resolution (see paper)
38% identity, 99% coverage: 2:268/269 of query aligns to 2:266/266 of 3ijpA
- active site: H155 (= H159), K159 (= K163)
- binding sodium ion: I21 (≠ A21), Q22 (≠ Y22), R24 (≠ D24), V27 (= V27)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G8), N10 (≠ M10), G11 (= G11), R12 (= R12), M13 (= M13), R35 (≠ S35), F75 (= F75), S76 (≠ A76), Q77 (≠ G77), A80 (≠ E80), G98 (= G101), T100 (= T103), G123 (≠ P126), N124 (= N127), M125 (= M128), F239 (= F241)
Q9X1K8 4-hydroxy-tetrahydrodipicolinate reductase; HTPA reductase; EC 1.17.1.8 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
36% identity, 99% coverage: 2:266/269 of query aligns to 1:212/216 of Q9X1K8
1vm6B Crystal structure of dihydrodipicolinate reductase (tm1520) from thermotoga maritima at 2.27 a resolution
36% identity, 99% coverage: 2:266/269 of query aligns to 6:217/218 of 1vm6B
- active site: H132 (= H159), K136 (= K163)
- binding nicotinamide-adenine-dinucleotide: G12 (= G8), S14 (≠ M10), G15 (= G11), R16 (= R12), M17 (= M13), D37 (= D47), V38 (≠ I48), F53 (= F75), S54 (≠ A76), S55 (= S78), E57 (= E80), A58 (= A81), G76 (= G101), T78 (= T103), Y101 (≠ P126), N102 (= N127), F103 (≠ M128), F192 (= F241)
5z2fA NADPH/pda bound dihydrodipicolinate reductase from paenisporosarcina sp. Tg-14 (see paper)
26% identity, 99% coverage: 1:266/269 of query aligns to 2:265/265 of 5z2fA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: R11 (≠ M10), G12 (= G11), K13 (≠ R12), M14 (= M13), D35 (≠ E34), H36 (= H42), K37 (≠ E43), L76 (≠ F75), T77 (≠ A76), G99 (= G101), T100 (= T102), T101 (= T103), P126 (= P126), N127 (= N127), F128 (≠ M128)
- binding pyridine-2,6-dicarboxylic acid: P126 (= P126), H155 (= H159), H156 (= H160), K159 (= K163), S164 (= S168), G165 (= G169), T166 (= T170), A215 (≠ G216)
5z2eA Dipicolinate bound dihydrodipicolinate reductase from paenisporosarcina sp. Tg-14 (see paper)
26% identity, 99% coverage: 1:266/269 of query aligns to 2:265/265 of 5z2eA
5wolA Crystal structure of dihydrodipicolinate reductase dapb from coxiella burnetii
26% identity, 99% coverage: 2:266/269 of query aligns to 2:230/230 of 5wolA
- active site: H133 (= H159), K137 (= K163)
- binding pyridine-2-carboxylic acid: P104 (= P126), T144 (= T170), K147 (= K173)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: N7 (≠ S7), G11 (= G11), K12 (≠ R12), M13 (= M13), G34 (≠ E34), R35 (≠ S35), F54 (= F75), T55 (≠ A76), T56 (≠ S78), S59 (≠ A81), G77 (= G101), T78 (= T102), T79 (= T103), P104 (= P126), N105 (= N127), F106 (≠ M128)
5eesA Crystal structure of dapb in complex with NADP+ from corynebacterium glutamicum (see paper)
27% identity, 99% coverage: 2:268/269 of query aligns to 2:247/247 of 5eesA
- active site: H133 (= H159), K137 (= K163)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G8), G11 (= G11), R12 (= R12), V13 (≠ M13), G34 (≠ E34), V35 (≠ S35), F53 (= F75), T54 (≠ A76), G76 (= G101), T78 (= T103), P104 (= P126), N105 (= N127), F106 (≠ M128), F220 (= F241)
- binding sulfate ion: H134 (= H160), K137 (= K163), K137 (= K163), G143 (= G169), T144 (= T170)
5eerA Crystal structure of dapb from corynebacterium glutamicum (see paper)
27% identity, 99% coverage: 2:268/269 of query aligns to 2:247/247 of 5eerA
Query Sequence
>WP_012675267.1 NCBI__GCF_000021565.1:WP_012675267.1
MVKVVISGIMGRMGRTIARLAYEDPEVQIAGGVESPDCVHFHENVKDILNKEDVDAPITA
DLSKIIERADVIIDFAGSTEAVLGHVRLAAADQNRKCMVIGTTGFSDEELEEIRQLSKDI
PIVLAPNMSIGVNLLFKLVEDAAKALKDKGFDIEVVEMHHRYKKDSPSGTAVKIVDILKE
ATGIQKVIYGREGIYENGRPSDEIAVFALRGGDVVGEHTVIFAGLGERIELTHRAGSRDI
FAKGAVEAAKWIKDKPPGLYDMMDVLGLK
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory