SitesBLAST
Comparing WP_012675348.1 NCBI__GCF_000021565.1:WP_012675348.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3al0C Crystal structure of the glutamine transamidosome from thermotoga maritima in the glutamylation state. (see paper)
44% identity, 100% coverage: 1:477/478 of query aligns to 102:562/564 of 3al0C
- active site: S110 (= S9), K335 (= K252)
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R106 (= R5), A108 (= A7), P109 (= P8), G118 (= G17), T122 (= T21), E142 (= E41), Y276 (= Y193), R294 (= R211), G295 (= G212), D297 (= D214), H298 (= H215), L324 (≠ I241), I325 (= I242), L333 (= L250)
- binding : T144 (= T43), D145 (= D44), R148 (= R47), Y208 (≠ C107), P213 (≠ L112), K252 (= K169), M255 (≠ V172), I266 (≠ V183), K269 (≠ R186), S270 (= S187), Y276 (= Y193), D297 (= D214), H298 (= H215), L299 (= L216), S300 (= S217), N301 (= N218), K304 (= K221), R330 (= R247), P332 (≠ K249), G363 (= G280), W364 (= W281), R365 (≠ H282), E370 (= E288), S387 (≠ H305), K389 (≠ A307), V391 (≠ A309), I392 (≠ V310), K397 (= K315), W400 (= W318), R407 (= R325), E446 (≠ D362), K447 (≠ S363), Q453 (≠ D369), I457 (≠ R373), R509 (≠ K424), K520 (= K435), Q524 (≠ M439), R527 (= R442), V535 (≠ S450), T536 (≠ G451), G538 (≠ D453), L539 (≠ M454)
Q8DLI5 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1) (see paper)
40% identity, 96% coverage: 2:461/478 of query aligns to 3:464/485 of Q8DLI5
- R6 (= R5) binding L-glutamate
- Y192 (= Y193) binding L-glutamate
2cfoA Non-discriminating glutamyl-tRNA synthetase from thermosynechococcus elongatus in complex with glu (see paper)
40% identity, 96% coverage: 2:461/478 of query aligns to 2:463/484 of 2cfoA
2cv2A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu) and an enzyme inhibitor, glu-ams (see paper)
39% identity, 99% coverage: 1:474/478 of query aligns to 1:467/468 of 2cv2A
- active site: K246 (= K252)
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R5 (= R5), A7 (= A7), S9 (= S9), G17 (= G17), I21 (≠ T21), E41 (= E41), Y187 (= Y193), R205 (= R211), A206 (≠ G212), E208 (≠ D214), W209 (≠ H215), L235 (≠ I241), L236 (≠ I242)
- binding : S9 (= S9), T43 (= T43), D44 (= D44), R47 (= R47), V145 (= V151), R163 (≠ K169), Y168 (≠ I174), E172 (= E178), V177 (= V183), K180 (≠ R186), S181 (= S187), Y187 (= Y193), E207 (= E213), E208 (≠ D214), W209 (≠ H215), V211 (≠ S217), R237 (≠ L243), K241 (≠ R247), L272 (= L278), M273 (≠ L279), G274 (= G280), E282 (= E288), S299 (≠ H305), P303 (≠ A309), V304 (= V310), K309 (= K315), W312 (= W318), R319 (= R325), P357 (≠ D362), R358 (≠ S363), R417 (≠ K424), Q432 (≠ M439), R435 (= R442), L442 (≠ T449), E443 (≠ S450), T444 (≠ G451), G446 (≠ D453), L447 (≠ M454), F448 (≠ A455)
2cv1A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu), atp, and an analog of l-glutamate: a quaternary complex
39% identity, 99% coverage: 1:474/478 of query aligns to 1:467/468 of 2cv1A
- active site: K246 (= K252)
- binding adenosine-5'-triphosphate: P8 (= P8), S9 (= S9), G17 (= G17), T18 (≠ N18), I21 (≠ T21), R47 (= R47), A206 (≠ G212), W209 (≠ H215), L235 (≠ I241), L236 (≠ I242)
- binding (4s)-4-amino-5-hydroxypentanoic acid: R5 (= R5), A7 (= A7), E41 (= E41), Y187 (= Y193), R205 (= R211), W209 (≠ H215)
- binding : S9 (= S9), E41 (= E41), T43 (= T43), D44 (= D44), R47 (= R47), V145 (= V151), R163 (≠ K169), V166 (= V172), E172 (= E178), V177 (= V183), K180 (≠ R186), S181 (= S187), Y187 (= Y193), E207 (= E213), E208 (≠ D214), W209 (≠ H215), V211 (≠ S217), R237 (≠ L243), K241 (≠ R247), K243 (= K249), M273 (≠ L279), G274 (= G280), S276 (≠ H282), E282 (= E288), S299 (≠ H305), P303 (≠ A309), V304 (= V310), K309 (= K315), W312 (= W318), R319 (= R325), P357 (≠ D362), R358 (≠ S363), R417 (≠ K424), L427 (≠ G434), Q432 (≠ M439), R435 (= R442), L442 (≠ T449), E443 (≠ S450), T444 (≠ G451), G446 (≠ D453), L447 (≠ M454), F448 (≠ A455)
2cuzA Glutamyl-tRNA synthetase from thermus thermophilus in complex with l- glutamate (see paper)
39% identity, 99% coverage: 1:474/478 of query aligns to 1:467/468 of 2cuzA
1n78A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with tRNA(glu) and glutamol-amp. (see paper)
39% identity, 99% coverage: 1:474/478 of query aligns to 1:467/468 of 1n78A
- active site: K246 (= K252)
- binding glutamol-amp: R5 (= R5), A7 (= A7), P8 (= P8), S9 (= S9), G17 (= G17), T18 (≠ N18), I21 (≠ T21), E41 (= E41), Y187 (= Y193), N191 (≠ V197), R205 (= R211), A206 (≠ G212), E208 (≠ D214), W209 (≠ H215), L235 (≠ I241), L236 (≠ I242)
- binding : S9 (= S9), T43 (= T43), D44 (= D44), R47 (= R47), V145 (= V151), R163 (≠ K169), V166 (= V172), Y168 (≠ I174), E172 (= E178), V177 (= V183), K180 (≠ R186), S181 (= S187), Y187 (= Y193), E207 (= E213), E208 (≠ D214), W209 (≠ H215), L210 (= L216), V211 (≠ S217), R237 (≠ L243), K241 (≠ R247), M273 (≠ L279), G274 (= G280), E282 (= E288), R297 (≠ D303), P303 (≠ A309), V304 (= V310), K309 (= K315), W312 (= W318), R319 (= R325), P357 (≠ D362), R358 (≠ S363), R417 (≠ K424), L427 (≠ G434), Q432 (≠ M439), R435 (= R442), L442 (≠ T449), E443 (≠ S450), T444 (≠ G451), G446 (≠ D453), L447 (≠ M454), F448 (≠ A455)
1j09A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with atp and glu (see paper)
39% identity, 99% coverage: 1:474/478 of query aligns to 1:467/468 of 1j09A
- active site: K246 (= K252)
- binding adenosine-5'-triphosphate: H15 (= H15), E208 (≠ D214), L235 (≠ I241), L236 (≠ I242), K243 (= K249), I244 (≠ L250), S245 (= S251), K246 (= K252), R247 (= R253)
- binding glutamic acid: R5 (= R5), A7 (= A7), S9 (= S9), E41 (= E41), Y187 (= Y193), N191 (≠ V197), R205 (= R211), W209 (≠ H215)
P27000 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
39% identity, 99% coverage: 1:474/478 of query aligns to 1:467/468 of P27000
- R358 (≠ S363) mutation to Q: Reduces affinity for tRNA and abolishes the ability to discriminate between tRNA(Glu) and tRNA(Gln).
1g59A Glutamyl-tRNA synthetase complexed with tRNA(glu). (see paper)
38% identity, 99% coverage: 1:474/478 of query aligns to 1:467/468 of 1g59A
- binding : D44 (= D44), R45 (= R45), A46 (≠ E46), R47 (= R47), P109 (= P109), V145 (= V151), R163 (≠ K169), V166 (= V172), E172 (= E178), V177 (= V183), K180 (≠ R186), S181 (= S187), D182 (= D188), E207 (= E213), E208 (≠ D214), R237 (≠ L243), K241 (≠ R247), T242 (≠ S248), K243 (= K249), M273 (≠ L279), G274 (= G280), E282 (= E288), S299 (≠ H305), L300 (≠ N306), P303 (≠ A309), V304 (= V310), K309 (= K315), W312 (= W318), R319 (= R325), P357 (≠ D362), R358 (≠ S363), R417 (≠ K424), K426 (= K433), L427 (≠ G434), Q432 (≠ M439), R435 (= R442), L442 (≠ T449), E443 (≠ S450), T444 (≠ G451), P445 (≠ V452), G446 (≠ D453), L447 (≠ M454), F448 (≠ A455)
P04805 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Escherichia coli (strain K12) (see 4 papers)
38% identity, 100% coverage: 2:478/478 of query aligns to 3:465/471 of P04805
- C98 (= C105) mutation to S: 10-fold decrease in activity. Strong decrease in zinc content.
- C100 (= C107) mutation to S: Loss of activity. Strong decrease in zinc content.; mutation to Y: Does not prevent zinc binding. Reduces only 2-fold the binding affinity for tRNA(Glu), but reduces more than 10-fold the affinity for glutamate in the presence of tRNA(Glu).
- C125 (= C132) mutation to S: Loss of activity. Strong decrease in zinc content.
- H127 (≠ N134) mutation to Q: 10-fold decrease in activity. Strong decrease in zinc content.
- H129 (≠ T136) mutation to Q: No change in activity or in zinc content.
- H131 (≠ D138) mutation to Q: No change in activity or in zinc content.
- H132 (≠ Q139) mutation to Q: No change in activity or in zinc content.
- C138 (≠ Y150) mutation to S: No change in activity or in zinc content.
- S239 (= S251) modified: Phosphoserine; mutation to D: Does not aminoacylate tRNA(Glu), not phosphorylated by HipA.
8i9iA Glutamyl-tRNA synthetase from escherichia coli bound to glutamate and zinc
38% identity, 100% coverage: 2:478/478 of query aligns to 3:465/468 of 8i9iA
4griB Crystal structure of a glutamyl-tRNA synthetase glurs from borrelia burgdorferi bound to glutamic acid and zinc (see paper)
39% identity, 100% coverage: 2:477/478 of query aligns to 2:482/485 of 4griB
- active site: S9 (= S9), K253 (= K252)
- binding glutamic acid: R5 (= R5), A7 (= A7), S9 (= S9), E41 (= E41), Y194 (= Y193), R212 (= R211), W216 (≠ H215)
- binding zinc ion: C105 (= C105), C107 (= C107), Y128 (= Y128), C132 (= C132)
8vc5A Crystal structure of glutamyl-tRNA synthetase glurs from pseudomonas aeruginosa (zinc bound)
37% identity, 100% coverage: 2:478/478 of query aligns to 4:470/488 of 8vc5A
6brlA Crystal structure of a glutamate tRNA ligase from elizabethkingia meningosepticum ccug26117 in complex with its amino acid (see paper)
36% identity, 99% coverage: 2:475/478 of query aligns to 3:499/502 of 6brlA
4g6zA Crystal structure of a glutamyl-tRNA synthetase glurs from burkholderia thailandensis bound to l-glutamate (see paper)
41% identity, 80% coverage: 2:383/478 of query aligns to 3:358/380 of 4g6zA
3aiiA Archaeal non-discriminating glutamyl-tRNA synthetase from methanothermobacter thermautotrophicus (see paper)
35% identity, 50% coverage: 2:238/478 of query aligns to 11:228/455 of 3aiiA
Sites not aligning to the query:
P27305 Glutamyl-Q tRNA(Asp) synthetase; Glu-Q-RSs; EC 6.1.1.- from Escherichia coli (strain K12) (see paper)
32% identity, 62% coverage: 5:298/478 of query aligns to 19:283/308 of P27305
- E55 (= E41) binding L-glutamate
- Y182 (= Y193) binding L-glutamate
- R200 (= R211) binding L-glutamate
4a91A Crystal structure of the glutamyl-queuosine trnaasp synthetase from e. Coli complexed with l-glutamate (see paper)
32% identity, 62% coverage: 5:298/478 of query aligns to 7:269/290 of 4a91A
- active site: S11 (= S9), K229 (= K252)
- binding glutamic acid: R7 (= R5), A9 (= A7), S11 (= S9), E43 (= E41), Y170 (= Y193), R188 (= R211), L192 (≠ H215)
- binding zinc ion: C99 (= C105), C101 (= C107), Y113 (= Y128), C117 (= C132)
P46655 Glutamate--tRNA ligase, cytoplasmic; Glutamyl-tRNA synthetase; (c)ERS; GluRS; P85; EC 6.1.1.17 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
27% identity, 53% coverage: 2:253/478 of query aligns to 202:441/708 of P46655
Sites not aligning to the query:
- 148 R→A: Abolishes interaction with ARC1.
Query Sequence
>WP_012675348.1 NCBI__GCF_000021565.1:WP_012675348.1
MVRVRFAPSPTGYLHLGNARTALFNYIYARHTGGKLILRIEDTDRERSKKEYEDLLIDDL
KWLGIEWDEGPDIGGDYGPYRQSERTDIYNQYVEKLKESGHIYKCFCSPEELEEERKKAL
SEGRPPRYSGKCRNLTEDQIKKLESEGKPYVWRFRVPDGEFIIFEDVIKGTVEINVDEFG
DFVIVRSDGSPVYNFVVVVDDALMKITDVIRGEDHLSNTPKQILIYRALGFEEPRFAHLP
IILGEDRSKLSKRHGAVSVRAFRDDGYVSEAMFNGLSLLGWHPKGDNEVLSKEEIIREFD
IKDIHNAPAVFDRAKLRWLNGVYIREKIDLDDLTERAVPFFEGFGYKADFDYYRKVMEAI
RDSLETLMDIEQRAKPFFVDDFHYEEDAQQFLKDETGYRVVQLFYEKVKDMDEIKKEDFK
RITKEIQKELGVKGKNLFMPIRVALTGVTSGVDMAVLVEVIGVERVKHRLQRALEYFG
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory