SitesBLAST
Comparing WP_012675810.1 NCBI__GCF_000021565.1:WP_012675810.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5xzaA Crystal structure of phosphofructokinase from staphylococcus aureus in complex with adp (see paper)
48% identity, 100% coverage: 1:324/324 of query aligns to 1:322/322 of 5xzaA
Q2FXM8 ATP-dependent 6-phosphofructokinase; ATP-PFK; Phosphofructokinase; Phosphohexokinase; EC 2.7.1.11 from Staphylococcus aureus (strain NCTC 8325 / PS 47) (see paper)
48% identity, 100% coverage: 1:324/324 of query aligns to 1:322/322 of Q2FXM8
- RC 72:73 (≠ RE 72:73) binding ATP
- GDGS 102:105 (≠ GNGS 102:105) binding ATP
- TID 127:129 (= TID 126:128) binding in other chain
- G150 (≠ D149) mutation to D: Exhibits higher affinity for fructose 6-phosphate and higher catalytic activity with a loss of dimer conversion; in association with A-151.
- L151 (≠ A150) mutation to A: Exhibits higher affinity for fructose 6-phosphate and higher catalytic activity with a loss of tetramer-dimer conversion; in association with D-150.
- R164 (= R163) mutation to A: Complete loss of fructose 6-phosphate binding.
- MGR 171:173 (= MGR 170:172) binding in other chain
- E224 (= E223) binding in other chain
- R245 (= R248) mutation to A: Complete loss of fructose 6-phosphate binding.
- HVQR 251:254 (≠ YIQR 254:257) binding in other chain
5xz9A Crystal structure of phosphofructokinase from staphylococcus aureus in complex with adenylylimidodiphosphate, the atp analogue (see paper)
48% identity, 100% coverage: 1:323/324 of query aligns to 1:321/321 of 5xz9A
- binding adenosine-5'-triphosphate: S9 (= S9), Y41 (≠ F41), C73 (≠ E73), F76 (= F76), K77 (≠ L77), G102 (= G102), D103 (≠ N103), G104 (= G104), S105 (= S105), R107 (≠ Q107), G108 (= G108)
5xz7A Crystal structure of phosphofructokinase from staphylococcus aureus in complex with adenylylimidodiphosphate, the atp analogue (see paper)
48% identity, 100% coverage: 1:324/324 of query aligns to 1:318/322 of 5xz7A
5xz6A Crystal structure of phosphofructokinase from staphylococcus aureus in complex with adenylylimidodiphosphate, the atp analogue (see paper)
48% identity, 100% coverage: 1:324/324 of query aligns to 1:318/318 of 5xz6A
6pfkA Phosphofructokinase, inhibited t-state (see paper)
45% identity, 100% coverage: 1:324/324 of query aligns to 1:319/319 of 6pfkA
- active site: G11 (= G11), R72 (= R72), C73 (≠ E73), D103 (≠ N103), G104 (= G104), G124 (≠ K125), T125 (= T126), D127 (= D128), D129 (= D130), R171 (= R172)
- binding 2-phosphoglycolic acid: R21 (= R21), R25 (= R25), G58 (= G58), D59 (≠ G59), R154 (= R155), R211 (= R212), K213 (= K214)
P00512 ATP-dependent 6-phosphofructokinase; ATP-PFK; Phosphofructokinase; Phosphohexokinase; EC 2.7.1.11 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see 2 papers)
45% identity, 100% coverage: 1:324/324 of query aligns to 1:319/319 of P00512
- RSVVR 21:25 (≠ RAVVR 21:25) binding ADP
- D59 (≠ G59) binding ADP
- RC 72:73 (≠ RE 72:73) binding ATP
- D103 (≠ N103) binding Mg(2+)
- TID 125:127 (= TID 126:128) binding in other chain
- R154 (= R155) binding in other chain
- R162 (= R163) binding substrate
- MGR 169:171 (= MGR 170:172) binding in other chain
- GAE 185:187 (≠ GAD 186:188) binding in other chain
- R211 (= R212) binding in other chain
- KKH 213:215 (≠ KKF 214:216) binding in other chain
- E222 (= E223) binding in other chain
- R243 (= R248) binding substrate
- HVQR 249:252 (≠ YIQR 254:257) binding in other chain
4pfkA Phosphofructokinase. Structure and control (see paper)
45% identity, 100% coverage: 1:324/324 of query aligns to 1:319/319 of 4pfkA
- active site: G11 (= G11), R72 (= R72), C73 (≠ E73), D103 (≠ N103), G104 (= G104), G124 (≠ K125), T125 (= T126), D127 (= D128), D129 (= D130), R171 (= R172)
- binding adenosine-5'-diphosphate: S9 (= S9), Y41 (≠ F41), R72 (= R72), C73 (≠ E73), F76 (= F76), K77 (≠ L77), G104 (= G104), G108 (= G108), R154 (= R155), G185 (= G186), R211 (= R212), G212 (= G213), K213 (= K214), H215 (≠ F216)
- binding 6-O-phosphono-beta-D-fructofuranose: D127 (= D128), M169 (= M170), E222 (= E223), H249 (≠ Y254), R252 (= R257)
- binding magnesium ion: G185 (= G186), E187 (≠ D188)
3pfkA Phosphofructokinase. Structure and control (see paper)
45% identity, 100% coverage: 1:324/324 of query aligns to 1:319/319 of 3pfkA
- active site: G11 (= G11), R72 (= R72), C73 (≠ E73), D103 (≠ N103), G104 (= G104), G124 (≠ K125), T125 (= T126), D127 (= D128), D129 (= D130), R171 (= R172)
- binding phosphate ion: R154 (= R155), K213 (= K214), H249 (≠ Y254), R252 (= R257)
1mtoA Crystal structure of a phosphofructokinase mutant from bacillus stearothermophilus bound with fructose-6-phosphate (see paper)
45% identity, 100% coverage: 1:324/324 of query aligns to 1:319/319 of 1mtoA
- active site: G11 (= G11), R72 (= R72), C73 (≠ E73), D103 (≠ N103), G104 (= G104), G124 (≠ K125), T125 (= T126), D127 (= D128), D129 (= D130), R171 (= R172)
- binding 6-O-phosphono-beta-D-fructofuranose: D127 (= D128), R162 (= R163), M169 (= M170), R171 (= R172), E222 (= E223), R243 (= R248), H249 (≠ Y254), R252 (= R257)
4i4iA Crystal structure of bacillus stearothermophilus phosphofructokinase mutant t156a bound to pep (see paper)
45% identity, 100% coverage: 1:324/324 of query aligns to 1:319/319 of 4i4iA
- active site: G11 (= G11), R72 (= R72), C73 (≠ E73), D103 (≠ N103), G104 (= G104), G124 (≠ K125), T125 (= T126), D127 (= D128), D129 (= D130), R171 (= R172)
- binding phosphoenolpyruvate: R21 (= R21), R25 (= R25), G58 (= G58), R154 (= R155), R211 (= R212), K213 (= K214), H215 (≠ F216)
1pfkA Crystal structure of the complex of phosphofructokinase from escherichia coli with its reaction products (see paper)
44% identity, 98% coverage: 1:316/324 of query aligns to 2:312/320 of 1pfkA
- active site: G12 (= G11), R73 (= R72), F74 (≠ E73), D104 (≠ N103), G105 (= G104), G125 (≠ K125), T126 (= T126), D128 (= D128), D130 (= D130), R172 (= R172)
- binding adenosine-5'-diphosphate: G11 (= G10), R22 (= R21), R26 (= R25), Y56 (≠ R55), S59 (≠ G58), D60 (≠ G59), R73 (= R72), F74 (≠ E73), F77 (= F76), R78 (≠ L77), G103 (= G102), D104 (≠ N103), G105 (= G104), S106 (= S105), M108 (≠ Q107), G109 (= G108), R155 (= R155), G213 (= G213), K214 (= K214), H216 (≠ F216)
- binding 1,6-di-O-phosphono-beta-D-fructofuranose: G12 (= G11), R73 (= R72), T126 (= T126), D128 (= D128), M170 (= M170), E223 (= E223), H250 (≠ Y254), R253 (= R257)
- binding magnesium ion: G186 (= G186), E188 (≠ D188)
P0A796 ATP-dependent 6-phosphofructokinase isozyme 1; ATP-PFK 1; Phosphofructokinase 1; 6-phosphofructokinase isozyme I; Phosphohexokinase 1; Sedoheptulose-7-phosphate kinase; EC 2.7.1.11 from Escherichia coli (strain K12) (see 2 papers)
44% identity, 98% coverage: 1:316/324 of query aligns to 2:312/320 of P0A796
- G12 (= G11) binding ATP
- RGVVR 22:26 (≠ RAVVR 21:25) binding ADP
- RYSVSD 55:60 (≠ PRDVGG 54:59) binding ADP
- RF 73:74 (≠ RE 72:73) binding ATP
- GDGS 103:106 (≠ GNGS 102:105) binding ATP
- D104 (≠ N103) binding Mg(2+)
- TID 126:128 (= TID 126:128) binding in other chain
- D128 (= D128) active site, Proton acceptor; mutation to S: 18000-fold reduction of catalytic rate.
- R155 (= R155) binding in other chain
- R163 (= R163) binding substrate
- R172 (= R172) mutation to S: 3.4-fold reduction in turnover numbers.
- GCE 186:188 (≠ GAD 186:188) binding in other chain
- KKH 214:216 (≠ KKF 214:216) binding in other chain
- E223 (= E223) binding in other chain
- R244 (= R248) binding substrate
- HIQR 250:253 (≠ YIQR 254:257) binding in other chain
P08237 ATP-dependent 6-phosphofructokinase, muscle type; ATP-PFK; PFK-M; 6-phosphofructokinase type A; Phosphofructo-1-kinase isozyme A; PFK-A; Phosphohexokinase; EC 2.7.1.11 from Homo sapiens (Human) (see 6 papers)
37% identity, 98% coverage: 2:317/324 of query aligns to 16:368/780 of P08237