SitesBLAST
Comparing WP_012675835.1 NCBI__GCF_000021565.1:WP_012675835.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
3kb6B Crystal structure of d-lactate dehydrogenase from aquifex aeolicus complexed with NAD and lactic acid (see paper)
44% identity, 84% coverage: 45:328/338 of query aligns to 41:318/334 of 3kb6B
- active site: S97 (≠ G101), R231 (= R235), D255 (= D260), E260 (= E265), H294 (= H304)
- binding lactic acid: F49 (= F53), S72 (= S76), V73 (≠ A77), G74 (= G78), Y96 (= Y100), R231 (= R235), H294 (= H304)
- binding nicotinamide-adenine-dinucleotide: V73 (≠ A77), Y96 (= Y100), V101 (= V105), G150 (= G153), R151 (= R154), I152 (= I155), D171 (= D174), V172 (≠ R175), P203 (= P207), T229 (= T233), A230 (≠ S234), R231 (= R235), H294 (= H304), A296 (= A306), Y297 (= Y307)
5z20F The ternary structure of d-lactate dehydrogenase from pseudomonas aeruginosa with nadh and oxamate (see paper)
37% identity, 99% coverage: 1:335/338 of query aligns to 8:332/336 of 5z20F
- active site: S108 (≠ G101), R241 (= R235), D265 (= D260), E270 (= E265), H302 (= H304)
- binding 1,4-dihydronicotinamide adenine dinucleotide: Y107 (= Y100), G160 (= G153), Q161 (≠ R154), I162 (= I155), Y180 (= Y173), D181 (= D174), P182 (≠ R175), C212 (≠ L206), P213 (= P207), T218 (= T212), T239 (= T233), G240 (≠ S234), R241 (= R235), H302 (= H304), A304 (= A306)
5z21B The ternary structure of d-lactate dehydrogenase from fusobacterium nucleatum with nadh and oxamate (see paper)
36% identity, 99% coverage: 2:334/338 of query aligns to 3:325/331 of 5z21B
- active site: S101 (≠ G101), R235 (= R235), D259 (= D260), E264 (vs. gap), H296 (= H304)
- binding 1,4-dihydronicotinamide adenine dinucleotide: Y100 (= Y100), I105 (≠ V105), G153 (= G153), K154 (≠ R154), I155 (= I155), D174 (= D174), L175 (≠ R175), P207 (= P207), T212 (= T212), T233 (= T233), G234 (≠ S234), R235 (= R235), H296 (= H304), Y299 (= Y307)
4zgsA Identification of the pyruvate reductase of chlamydomonas reinhardtii (see paper)
35% identity, 89% coverage: 28:328/338 of query aligns to 36:335/346 of 4zgsA
- active site: S111 (≠ G101), R244 (= R235), D268 (= D260), E273 (= E265), H311 (= H304)
- binding nicotinamide-adenine-dinucleotide: Y110 (= Y100), G163 (= G153), A164 (≠ R154), I165 (= I155), D184 (= D174), C215 (≠ L206), P216 (= P207), L218 (≠ T209), S220 (= S211), T221 (= T212), S243 (= S234), H311 (= H304), F314 (≠ Y307)
5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
37% identity, 84% coverage: 45:327/338 of query aligns to 44:311/334 of 5aovA
- active site: L100 (≠ G101), R241 (= R235), D265 (= D260), E270 (= E265), H288 (= H304)
- binding glyoxylic acid: M52 (≠ F53), L53 (≠ I54), L53 (≠ I54), Y74 (≠ R75), A75 (≠ S76), V76 (≠ A77), G77 (= G78), R241 (= R235), H288 (= H304)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (≠ A77), T104 (≠ V105), F158 (≠ T152), G159 (= G153), R160 (= R154), I161 (= I155), S180 (≠ D174), R181 (= R175), A211 (≠ H205), V212 (≠ L206), P213 (= P207), T218 (= T212), I239 (≠ T233), A240 (≠ S234), R241 (= R235), H288 (= H304), G290 (≠ A306)
1wwkA Crystal structure of phosphoglycerate dehydrogenase from pyrococcus horikoshii ot3
43% identity, 88% coverage: 20:315/338 of query aligns to 17:289/304 of 1wwkA
- active site: S96 (≠ G101), R230 (= R235), D254 (= D260), E259 (= E272), H278 (= H304)
- binding nicotinamide-adenine-dinucleotide: V100 (= V105), G146 (= G151), F147 (≠ T152), G148 (= G153), R149 (= R154), I150 (= I155), Y168 (= Y173), D169 (= D174), P170 (≠ R175), V201 (≠ L206), P202 (= P207), T207 (= T212), T228 (= T233), S229 (= S234), D254 (= D260), H278 (= H304), G280 (≠ A306)
4cukA Structure of salmonella d-lactate dehydrogenase in complex with nadh
30% identity, 92% coverage: 1:312/338 of query aligns to 1:303/330 of 4cukA
- active site: S101 (≠ G101), R234 (= R235), D258 (= D260), E263 (= E265), H295 (= H304)
- binding 1,4-dihydronicotinamide adenine dinucleotide: Y100 (= Y100), G153 (= G153), K154 (≠ R154), I155 (= I155), F173 (≠ Y173), D174 (= D174), P175 (≠ R175), H204 (= H205), C205 (≠ L206), P206 (= P207), N211 (≠ T212), T232 (= T233), Y260 (≠ F262), H295 (= H304), A297 (= A306)
4xkjA A novel d-lactate dehydrogenase from sporolactobacillus sp
35% identity, 98% coverage: 1:331/338 of query aligns to 1:322/332 of 4xkjA
- active site: S102 (≠ G101), R234 (= R235), D258 (= D260), E263 (= E265), H295 (= H304)
- binding nicotinamide-adenine-dinucleotide: Y101 (= Y100), V106 (= V105), G152 (= G151), G154 (= G153), R155 (= R154), I156 (= I155), D175 (= D174), I176 (≠ R175), R179 (≠ K178), H204 (= H205), V205 (≠ L206), P206 (= P207), T211 (= T212), A232 (≠ T233), R234 (= R235), H295 (= H304), G297 (≠ A306), F298 (≠ Y307)
8grvA Dictyostelium discoideum lactate dehydrogenase (dicldha)with NAD
31% identity, 89% coverage: 28:327/338 of query aligns to 27:321/336 of 8grvA
- binding nicotinamide-adenine-dinucleotide: V106 (= V105), G154 (= G153), N155 (≠ R154), I156 (= I155), D176 (= D174), I177 (≠ R175), I178 (≠ T176), T208 (≠ L206), P209 (= P207), T214 (= T212), V235 (≠ T233), H298 (= H304), A300 (= A306), W301 (≠ Y307)
6biiA Crystal structure of pyrococcus yayanosii glyoxylate hydroxypyruvate reductase in complex with NADP and malonate (re-refinement of 5aow) (see paper)
36% identity, 84% coverage: 45:327/338 of query aligns to 43:310/332 of 6biiA
- active site: L99 (≠ G101), R240 (= R235), D264 (= D260), E269 (= E265), H287 (= H304)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V75 (≠ A77), T103 (≠ V105), G156 (= G151), F157 (≠ T152), G158 (= G153), R159 (= R154), I160 (= I155), A179 (≠ D174), R180 (= R175), S181 (≠ T176), K183 (= K178), V211 (≠ L206), P212 (= P207), E216 (≠ S211), T217 (= T212), V238 (≠ T233), A239 (≠ S234), R240 (= R235), D264 (= D260), H287 (= H304), G289 (≠ A306)
2eklA Structure of st1218 protein from sulfolobus tokodaii
32% identity, 93% coverage: 18:330/338 of query aligns to 19:308/312 of 2eklA
- active site: S100 (≠ G101), R232 (= R235), D256 (= D260), E261 (= E265), H282 (= H304)
- binding nicotinamide-adenine-dinucleotide: I76 (≠ A77), S100 (≠ G101), G148 (= G151), G150 (= G153), R151 (= R154), I152 (= I155), Y170 (= Y173), D171 (= D174), I172 (≠ R175), L173 (≠ T176), H202 (= H205), V203 (≠ L206), T204 (≠ P207), I212 (≠ L215), T230 (= T233), S231 (= S234), D256 (= D260), G284 (≠ A306)
6plfA Crystal structure of human phgdh complexed with compound 1 (see paper)
34% identity, 80% coverage: 45:316/338 of query aligns to 42:291/305 of 6plfA
7dkmA Phgdh covalently linked to oridonin (see paper)
34% identity, 80% coverage: 45:316/338 of query aligns to 42:291/306 of 7dkmA
- binding nicotinamide-adenine-dinucleotide: T74 (≠ A77), A102 (≠ V105), G148 (= G151), R151 (= R154), I152 (= I155), Y170 (= Y173), D171 (= D174), P172 (≠ R175), I173 (≠ T176), H202 (= H205), T203 (≠ L206), P204 (= P207), T209 (= T212), C230 (≠ T233), A231 (≠ S234), R232 (= R235), H279 (= H304), G281 (≠ A306)
Sites not aligning to the query:
- binding (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14R,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one: 14, 17, 18, 293
6cwaA Crystal structure phgdh in complex with nadh and 3-phosphoglycerate at 1.77 a resolution (see paper)
34% identity, 80% coverage: 45:316/338 of query aligns to 40:289/299 of 6cwaA
- binding 1,4-dihydronicotinamide adenine dinucleotide: N96 (≠ G101), A100 (≠ V105), R149 (= R154), I150 (= I155), Y168 (= Y173), D169 (= D174), P170 (≠ R175), I171 (≠ T176), H200 (= H205), T201 (≠ L206), P202 (= P207), T207 (= T212), C228 (≠ T233), A229 (≠ S234), R230 (= R235), H277 (= H304), G279 (≠ A306)
6rj2A Crystal structure of phgdh in complex with compound 40 (see paper)
34% identity, 80% coverage: 45:316/338 of query aligns to 38:287/299 of 6rj2A
- binding ~{N}-[(1~{R})-1-[4-(ethanoylsulfamoyl)phenyl]ethyl]-2-methyl-5-phenyl-pyrazole-3-carboxamide: G146 (= G153), I148 (= I155), Y166 (= Y173), D167 (= D174), P168 (≠ R175), I169 (≠ T176), I170 (≠ K177), H198 (= H205), T199 (≠ L206), L208 (= L215), R228 (= R235)
6plgA Crystal structure of human phgdh complexed with compound 15 (see paper)
34% identity, 80% coverage: 45:316/338 of query aligns to 41:290/303 of 6plgA
7ewhA Crystal structure of human phgdh in complex with homoharringtonine (see paper)
34% identity, 80% coverage: 45:316/338 of query aligns to 41:290/302 of 7ewhA
- binding (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine: L146 (≠ I150), G147 (= G151), L148 (≠ T152), G149 (= G153), R150 (= R154), I151 (= I155), G152 (= G156), D170 (= D174), H201 (= H205), T202 (≠ L206), P203 (= P207)
6rihA Crystal structure of phgdh in complex with compound 9 (see paper)
34% identity, 80% coverage: 45:316/338 of query aligns to 41:290/302 of 6rihA
6rj3A Crystal structure of phgdh in complex with compound 15 (see paper)
34% identity, 80% coverage: 45:316/338 of query aligns to 40:289/297 of 6rj3A
6rj5A Crystal structure of phgdh in complex with compound 39 (see paper)
34% identity, 80% coverage: 45:316/338 of query aligns to 41:290/301 of 6rj5A
Query Sequence
>WP_012675835.1 NCBI__GCF_000021565.1:WP_012675835.1
MKIYFFGIEDWEKLHIERKLKEAGIEGDIKFTKEPLSIDNVDLYKDADIISVFIYSKVTK
EVIDRMENLRLIVTRSAGYDHIDVSYAKEKGIYVAYIPGYGNNTVAEYTFALILALSRKF
KPMIDRTARGVFSREGLMGIDLLGKTIGIIGTGRIGCYVARLAHGFGMKILAYDRTKKKE
IIEKYGVEYVGLEELLMRSDIVTIHLPYTKSTHYLINRFNIKLMKLNAMLINTSRGAVVE
IDAVVEALKEGRLAGGVALDTFEAEEVLIEEEFLRRDDIPAIKLKKAMEAYYVLHEDNVI
VSPHLAYYTKDASERILDIAVDEIKHFLTEGKPVNIAQ
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory