SitesBLAST

Q96XT4 2-oxoacid:ferredoxin oxidoreductase 2, subunit beta; OFOR2; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
43% identity, 84% coverage: 23:282/308 of query aligns to 11:268/304 of Q96XT4

query
sites
Q96XT4

W

W

C
|
C

P

P

G

G

C
|
C

G

G

D

D

F

F

G

G

V

I

V

L

T

R

A

A

L

E

T

E

K

M

A

A

F

I

S

R

E

E

E

L

R

G

F

I

D

N

P

P

T

K

A

S

I

V

T

V

M

I

T

V

S

S

G

G

I
|
I

G
|
G

C
|
C

S
|
S

S

G

R

K

L

I

P

P

L

H

W

F

M

M

N

N

-

L

-

P

A

I

F

S

G

G

I

V

H

H

T

T

A

L

H

H

G

G

R

R

A

S

L

I

P

A

A

F

A

A

V

T

G

G

V

I

R

K

L

L

A

S

R

N

P

P

E

S

T

L

P

E

T

V

I

I

V

V

T

N

A

V

G

G

D
|
D

G
|
G

D
|
D

I

G

F

L

S

G

I

I

G

G

M

M

E

G

H

H

F

F

P

V

H

H

T

L

A

G

R

R

K

R

N

N

F

I

D

D

I

I

T

A

L

V

I

L

V

V

M

H

D

N

N
|
N

R

G

M
x
V

Y

Y

A
x
G

L
|
L

T

T

K

K

N

G

Q

Q

T

A

S

S

P

P

T

T

S

L

R

H

H

R

G

G

Y

E

K

K

G

T

S

K

L

S

N

L

P

P

Y

K

G

P

N

N

I

I

E

M

D

D

P

A

F

V

N

N

V

P

I

L

K

A

F

V

A

A

I

L

A

A

S

A

G

G

A

Y

T

T

F

F

V

V

A

A

Q

R

S

G

Y

Y

S

A

G

Y

N

D

P

V

K

M

H

H

L

L

A

K

E

E

T

L

I

I

Q

K

A

K

A

A

V

I

E

L

H

H

K

K

G

G

F

S

S

A

F

L

V

V

N

D

V

I

L

L

S

Q

P

P

C
|
C

P

P

T

T

F

Y

N

N

K

D

I

I

D

N

T

T

F

K

K

E

Y

W

Y

Y

K

D

G

K

R

R

L

V

I

Y

D

K

I

L

N

D

K

N

E

V

L

P

G

G

H

W

D

D

P

P

S

V

D

V

M

R

K

K

A

E

L

-

E

E

L

A

A

Q

S

K

H

K

V

F

L

E

D

Q

A

A

D

I

Y

M

P

K

E

S

L

Y

E

E

D

W

A

G

E

E

P

-

F

-

K

-

G

-

K

K

R

I

P

P

I

I

G

G

I

I

F

F

Y

Y

K

Q

V

N

E

E

-

L

K

V

E

P

T

T

F

F

E

E

E

D

R

R

I

L

C12 (= C24) binding [4Fe-4S] cluster
C15 (= C27) binding [4Fe-4S] cluster
IGCS 44:47 (= IGCS 56:59) binding thiamine diphosphate
C46 (= C58) binding [4Fe-4S] cluster
D90 (= D100) binding Mg(2+)
GD 91:92 (= GD 101:102) binding thiamine diphosphate
N118 (= N128) binding Mg(2+)
V120 (≠ M130) binding Mg(2+)
GL 122:123 (≠ AL 132:133) binding thiamine diphosphate
C197 (= C207) binding [4Fe-4S] cluster

5b46B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - ligand free form (see paper)
43% identity, 84% coverage: 23:282/308 of query aligns to 8:265/301 of 5b46B

query
sites
5b46B

W
|
W

C
|
C

P

P

G

G

C
|
C

G

G

D

D

F

F

G

G

V

I

V

L

T

R

A

A

L

E

T

E

K

M

A

A

F

I

S

R

E

E

E

L

R

G

F

I

D

N

P

P

T

K

A

S

I

V

T

V

M

I

T

V

S

S

G

G

I
|
I

G
|
G

C
|
C

S
|
S

S

G

R

K

L

I

P

P

L

H

W

F

M

M

N

N

-

L

-

P

A

I

F

S

G

G

I

V

H

H

T

T

A

L

H
|
H

G

G

R

R

A

S

L

I

P

A

A

F

A

A

V

T

G

G

V

I

R

K

L

L

A

S

R

N

P

P

E

S

T

L

P

E

T

V

I

I

V

V

T

N

A

V

G
|
G

D
|
D

G
|
G

D
|
D

I

G

F

L

S

G

I

I

G

G

M

M

E

G

H

H

F

F

P

V

H

H

T

L

A

G

R

R

K

R

N

N

F

I

D

D

I

I

T

A

L

V

I

L

V

V

M

H

D

N

N
|
N

R

G

M
x
V

Y
|
Y

A
x
G

L
|
L

T
|
T

K

K

N

G

Q

Q

T

A

S

S

P

P

T

T

S

L

R

H

H

R

G

G

Y

E

K

K

G

T

S

K

L

S

N

L

P

P

Y

K

G

P

N

N

I

I

E

M

D

D

P

A

F

V

N

N

V

P

I

L

K

A

F

V

A

A

I

L

A

A

S

A

G

G

A

Y

T

T

F

F

V

V

A

A

Q

R

S

G

Y

Y

S

A

G

Y

N

D

P

V

K

M

H

H

L

L

A

K

E

E

T

L

I

I

Q

K

A

K

A

A

V

I

E

L

H

H

K

K

G

G

F

S

S

A

F

L

V

V

N

D

V

I

L

L

S

Q

P

P

C
|
C

P

P

T
|
T

F
x
Y

N

N

K

D

I

I

D

N

T

T

F

K

K

E

Y

W

Y

Y

K

D

G

K

R

R

L

V

I

Y

D

K

I

L

N

D

K

N

E

V

L

P

G

G

H

W

D

D

P

P

S

V

D

V

M

R

K

K

A

E

L

-

E

E

L

A

A

Q

S

K

H

K

V

F

L

E

D

Q

A

A

D

I

Y

M

P

K

E

S

L

Y

E

E

D

W

A

G

E

E

P

-

F

-

K

-

G

-

K

K

R

I

P

P

I

I

G

G

I

I

F

F

Y

Y

K

Q

V

N

E

E

-

L

K

V

E

P

T

T

F

F

E

E

E

D

R

R

I

L

binding magnesium ion: D87 (= D100), N115 (= N128), V117 (≠ M130)
binding iron/sulfur cluster: W8 (= W23), C9 (= C24), C12 (= C27), C43 (= C58), C194 (= C207), T196 (= T209), Y197 (≠ F210)
binding thiamine diphosphate: I41 (= I56), G42 (= G57), C43 (= C58), S44 (= S59), H62 (= H75), G86 (= G99), G88 (= G101), D89 (= D102), N115 (= N128), V117 (≠ M130), Y118 (= Y131), G119 (≠ A132), L120 (= L133), T121 (= T134)

Q96Y68 2-oxoacid:ferredoxin oxidoreductase 1, subunit beta; OFOR1; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 2 papers)
41% identity, 84% coverage: 23:282/308 of query aligns to 11:268/305 of Q96Y68

query
sites
Q96Y68

W

W

C
|
C

P

P

G

G

C
|
C

G

G

D

N

F

F

G

G

V

I

V

L

T

N

A

A

L

E

T

Q

K

Q

A

A

F

I

S

V

E

E

E

L

R

G

F

V

D

D

P

T

T

K

A

N

I

V

T

V

M

V

T

V

S

S

G

G

I

I

G

G

C
|
C

S

S

S

G

R
x
K

L

I

P

P

L

H

W

F

M

F

N

R

A

T

-

P

-

I

F

S

G

G

I

V

H

H

T

T

A

L

H

H

G

G

R

R

A

A

L

I

P

A

A

F

A

A

V

T

G

G

V

I

R

K

L

L

A

S

R

N

P

P

E

D

T

L

P

V

T

V

I

I

V

V

T

N

A

G

G

G

D
|
D

G
|
G

D
|
D

I

L

F

L

S

G

I

I

G

G

M

A

E

G

H

H

F

F

P

V

H

A

T

A

A

G

R

R

K

R

N

N

F

V

D

D

I

M

T

V

L

V

I

I

V

L

M

H

D

D

N
|
N

R

G

M
x
V

Y

Y

A
x
G

L
|
L

T

T

K
|
K

N

G

Q

Q

T

A

S

S

P

P

T

T

S

L

R

K

H

R

G

G

Y

E

K

K

G

P

S

K

L

S

N

L

P

P

Y

R

G

P

N

N

I

I

E

N

D

D

P

A

F

V

N

N

V

P

I

I

K

A

F

L

A

A

I

I

A

S

S

S

G

G

A

Y

T

T

F

F

V

V

A

A

Q

R

S

G

Y

Y

S

A

G

Y

N

D

P

V

K
|
K

H

H

L

L

A

K

E

E

T

L

I

I

Q

K

A

S

A

A

V

I

E

K

H

H

K

K

G

G

F

L

S

A

F

L

V

I

N

D

V

V

L

L

S

Q

P

P

C
|
C

P

P

T

T

F

Y

N

N

K

D

I

I

D

N

T

T

F

K

K

E

Y

W

Y

Y

K

D

G

K

R

R

L

I

I

Y

D

K

I

L

N

D

K

T

E

L

L

P

G

D

H

W

D

D

P

P

S

V

D

-

M

V

K

K

A

K

A

P

L

E

E

E

L

V

A

N

S

E

H

K

V

I

L

K

D

R

A

A

D

I

Y

D

P

K

E

S

L

L

E

E

D

-

A

-

E

-

P

-

F

W

K

G

G

D

K

R

R

I

P

P

I

I

G

G

I

I

F

F

Y

Y

K

Q

V

N

E

E

-

L

K

V

E

P

T

S

F

Y

E

E

R

R

I

I

C12 (= C24) binding [4Fe-4S] cluster
C15 (= C27) binding [4Fe-4S] cluster
C46 (= C58) binding [4Fe-4S] cluster
K49 (≠ R61) mutation to I: Loss of oxidoreductase activity toward 2-oxoglutarate but retains its activity toward pyruvate.
D90 (= D100) binding Mg(2+)
GD 91:92 (= GD 101:102) binding thiamine diphosphate
N118 (= N128) binding Mg(2+)
V120 (≠ M130) binding Mg(2+)
GL 122:123 (≠ AL 132:133) binding thiamine diphosphate
K125 (= K135) Plays an important role in the binding of CoA; mutation to A: Shows a strong decrease of affinity for CoA and a poor inactivation by 4-fluoro-7-nitrobenzofurazan (NBD-F).
K173 (= K183) mutation to A: Same oxidoreductase activity as the wild-type.
C197 (= C207) binding [4Fe-4S] cluster

P72579 2-oxoacid:ferredoxin oxidoreductase subunit beta; OFOR; EC 1.2.7.11 from Sulfolobus sp. (see paper)
41% identity, 84% coverage: 23:282/308 of query aligns to 11:268/305 of P72579

query
sites
P72579

W

W

C

C

P

P

G

G

C

C

G

G

D

N

F

F

G

G

V

I

V

L

T

N

A

A

L

E

T

Q

K

Q

A

A

F

I

S

V

E

E

E

L

R

G

F

V

D

D

P

T

T

K

A

N

I

V

T

V

M

V

T

V

S

S

G

G

I

I

G

G

C

C

S

S

S

G

R
x
K

L

I

P

P

L

H

W

F

M

F

N

R

A

T

-

P

-

I

F

S

G

G

I

V

H

H

T

T

A

L

H

H

G

G

R

R

A

A

L

I

P

A

A

F

A

A

V

T

G

G

V

I

R

K

L

L

A

S

R

N

P

P

E

D

T

L

P

V

T

V

I

I

V

V

T

N

A

G

G

G

D

D

G

G

D

D

I

L

F

L

S

G

I

I

G

G

M

A

E

G

H

H

F

F

P

V

H

A

T

A

A

G

R

R

K

R

N

N

F

V

D

D

I

M

T

V

L

V

I

I

V

L

M

H

D

D

N

N

R

G

M

V

Y

Y

A

G

L
|
L

T

T

K

K

N

G

Q

Q

T

A

S

S

P

P

T

T

S

L

R

K

H

R

G

G

Y

E

K

K

G

P

S

K

L

S

N

L

P

P

Y

R

G

P

N

N

I

I

E

N

D

D

P

A

F

V

N

N

V

P

I

I

K

A

F

L

A

A

I

I

A

S

S

S

G

G

A

Y

T

T

F

F

V

V

A

A

Q

R

S

G

Y

Y

S

A

G

Y

N

D

P

V

K

K

H

H

L

L

A

K

E

E

T

L

I

I

Q

K

A

S

A

A

V

I

E

K

H

H

K

K

G

G

F

L

S

A

F

L

V

I

N

D

V

V

L

L

S

Q

P

P

C

C

P

P

T

T

F

Y

N

N

K

D

I

I

D

N

T

T

F

K

K

E

Y

W

Y

Y

K

D

G

K

R

R

L

I

I

Y

D

K

I

L

N

D

K

T

E

L

L

P

G

D

H

W

D

D

P

P

S

V

D

-

M

V

K

K

A

K

A

P

L

E

E

E

L

V

A

N

S

E

H

K

V

I

L

K

D

R

A

A

D

I

Y

D

P

K

E

S

L

L

E

E

D

-

A

-

E

-

P

-

F

W

K

G

G

D

K

R

R

I

P

P

I

I

G

G

I

I

F

F

Y

Y

K

Q

V

N

E

E

-

L

K

V

E

P

T

S

F

Y

E

E

R

R

I

I

K49 (≠ R61) mutation to I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to R: Increase the oxidoreductase activity with pyruvate.; mutation to V: Slight decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.
L123 (= L133) mutation L->A,I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to N: Strong decrease of the oxidoreductase activity with pyruvate and 2-oxobutyrate. However, this mutant shows almost the same activity with 2-oxoglutarate as the wild-type.

5b48B 2-oxoacid:ferredoxin oxidoreductase 1 from sulfolobus tokodai (see paper)
41% identity, 84% coverage: 23:282/308 of query aligns to 7:250/286 of 5b48B

query
sites
5b48B

W

W

C
|
C

P

P

G

G

C
|
C

G

G

D

N

F

F

G

G

V

I

V

L

T

N

A

A

L

E

T

Q

K

Q

A

A

F

I

S

V

E

E

E

L

R

G

F

V

D

D

P

T

T

K

A

N

I

V

T

V

M

V

T

V

S

S

G

G

I
|
I

G
|
G

C
|
C

S
|
S

S

G

R

K

L

I

P

P

L

H

W

F

M

T

N

P

A

I

F

S

G

G

I

V

H

H

T

T

A

L

H
|
H

G

G

R

R

A

A

L

I

P

A

A

F

A

A

V

T

G

G

V

I

R

K

L

L

A

S

R

N

P

P

E

D

T

L

P

V

T

V

I

I

V

V

T

N

A

G

G

G

D
|
D

G
|
G

D
|
D

I

L

F

L

S

G

I

I

G

G

M

A

E

G

H

H

F

F

P

V

H

A

T

A

A

G

R

R

K

R

N

N

F

V

D

D

I

M

T

V

L

V

I

I

V

L

M

H

D

D

N
|
N

R

G

M
x
V

Y
|
Y

A
x
G

L
|
L

T

T

K

K

N

G

Q

Q

T

A

S

S

P

P

T

T

S

L

R

K

H

R

G

G

Y

E

K

-

G

-

S

-

L

-

N

-

P

-

Y

-

G

-

N

N

I

I

E

N

D

D

P

A

F

V

N

N

V

P

I

I

K

A

F

L

A

A

I

I

A

S

S

S

G

G

A

Y

T

T

F

F

V

V

A

A

Q

R

S

G

Y

Y

S

A

G

Y

N

D

P

V

K

K

H

H

L

L

A

K

E

E

T

L

I

I

Q

K

A

S

A

A

V

I

E

K

H

H

K

K

G

G

F

L

S

A

F

L

V

I

N

D

V

V

L

L

S

Q

P

P

C
|
C

P

P

T
|
T

F

Y

N

N

K

D

I

I

D

N

T

T

F

K

K

E

Y

W

Y

-

K

-

G

-

R

R

L

I

I

Y

D

K

I

L

N

D

K

T

E

L

L

P

G

D

H

W

D

D

P

P

S

V

D

-

M

V

K

K

A

K

A

P

L

E

E

E

L

V

A

N

S

E

H

K

V

I

L

K

D

R

A

A

D

I

Y

D

P

K

E

S

L

L

E

E

D

-

A

-

E

-

P

-

F

-

K

W

G

G

K

D

R

I

P

P

I

I

G

G

I

I

F

F

Y

Y

K

Q

V

N

E

E

-

L

K

V

E

P

T

S

F

Y

E

E

R

R

I

I

binding magnesium ion: D84 (= D100), N112 (= N128), V114 (≠ M130)
binding iron/sulfur cluster: C8 (= C24), C11 (= C27), C42 (= C58), C183 (= C207), T185 (= T209)
binding 2-[(2E)-3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-(1-oxidanylpropylidene)-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: I40 (= I56), G41 (= G57), C42 (= C58), S43 (= S59), H59 (= H75), G85 (= G101), D86 (= D102), N112 (= N128), V114 (≠ M130), Y115 (= Y131), G116 (≠ A132), L117 (= L133)

5b47B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - pyruvate complex (see paper)
40% identity, 84% coverage: 23:282/308 of query aligns to 4:239/275 of 5b47B

query
sites
5b47B

W

W

C
|
C

P

P

G

G

C
|
C

G

G

D

D

F

F

G

G

V

I

V

L

T

R

A

A

L

E

T

E

K

M

A

A

F

I

S

R

E

E

E

L

R

G

F

I

D

N

P

P

T

K

A

S

I

V

T

V

M

I

T

V

S

S

G

G

I
|
I

G
|
G

C
|
C

S
|
S

S

G

R

K

L

I

P

P

L

H

W

F

M

M

N

N

-

L

-

P

A

I

F

S

G

G

I

V

H

H

T

T

A

L

H
|
H

G

G

R

R

A

S

L

I

P

A

A

F

A

A

V

T

G

G

V

I

R

K

L

L

A

S

R

N

P

P

E

S

T

L

P

E

T

V

I

I

V

V

T

N

A

V

G

G

D
|
D

G
|
G

D
|
D

I

G

F

L

S

G

I

I

G

G

M

M

E

G

H

H

F

F

P

V

H

H

T

L

A

G

R

R

K

R

N

N

F

I

D

D

I

I

T

A

L

V

I

L

V

V

M

H

D

N

N
|
N

R

G

M
x
V

Y
|
Y

A

G

L
|
L

T

-

K

-

N

-

Q

Q

T

A

S

S

P

P

T

T

S

L

R

H

H

R

G

G

Y

E

K

-

G

-

S

-

L

-

N

-

P

-

Y

-

G

-

N

N

I

I

E

M

D

D

P

A

F

V

N

N

V

P

I

L

K

A

F

V

A

A

I

L

A

A

S

A

G

G

A

Y

T

T

F

F

V

V

A

A

Q

R

S

G

Y

Y

S

A

G

Y

N

D

P

V

K

M

H

H

L

L

A

K

E

E

T

L

I

I

Q

K

A

K

A

A

V

I

E

L

H

H

K

K

G

G

F

S

S

A

F

L

V

V

N

D

V

I

L

L

S

Q

P

P

C
|
C

P

P

T

T

F

Y

N

-

K

-

I

-

D

-

T

-

F

-

K

-

Y

-

K

-

G

-

R

R

L

V

I

Y

D

K

I

L

N

D

K

N

E

V

L

P

G

G

H

W

D

D

P

P

S

V

D

V

M

R

K

K

A

E

L

-

E

E

L

A

A

Q

S

K

H

K

V

F

L

E

D

Q

A

A

D

I

Y

M

P

K

E

S

L

Y

E

E

D

W

A

G

E

E

P

-

F

-

K

-

G

-

K

K

R

I

P

P

I

I

G

G

I

I

F

F

Y

Y

K

Q

V

N

E

E

-

L

K

V

E

P

T

T

F

F

E

E

E

D

R

R

I

L

binding magnesium ion: D83 (= D100), N111 (= N128)
binding iron/sulfur cluster: C5 (= C24), C8 (= C27), C39 (= C58), C179 (= C207)
binding thiamine diphosphate: I37 (= I56), G38 (= G57), C39 (= C58), S40 (= S59), H58 (= H75), G84 (= G101), D85 (= D102), N111 (= N128), V113 (≠ M130), Y114 (= Y131), L116 (= L133)

5exeC Crystal structure of oxalate oxidoreductase from moorella thermoacetica bound with carboxy-tpp adduct (see paper)
26% identity, 68% coverage: 1:208/308 of query aligns to 2:226/314 of 5exeC

query
sites
5exeC

M

L

E

D

Y

R

I

I

K

A

L

S

E

I

E

K

K

K

L

A

P

P

P

D

K

E

E

E

Y

Y

R

Y

S

V

D

P

I

G

E

H

P

R

T

T

W

-

C
|
C

P

A

G

G

C
|
C

G

G

D
x
P

F

-

G

-

V

-

T

-

A

A

L

L

T

T

K

Y

A

R

F

L

S

V

E

A

E

K

R

A

F

A

D

G

P

P

T

N

A

T

I

I

-

F

-

I

-

G

-

P

-
x
T

-
x
G

-
x
C

-

M

-

Y

T

V

M

A

T

N

S

T

G

S

I

Y

G

G

C

C

S

G

S

P

R

W

L

R

P

V

L

P

W

W

M

I

-

H

-

A

-

Q

-
x
I

-

T

N

N

A

G

F

G

G

A

I

V

H

A

T

S

A

G

H

I

G

E

R

A

A

A

L

Y

P

K

A

A

A

M

V

I

G

R

V

K

R

K

L

K

A

T

R

D

P

A

E

E

T

F

P

P

T

N

I

I

-

I

V

V

T

M

A

A

G
|
G

D
|
D

G
|
G

D

G

I

A

F

V

S

D

I

I

G

G

M

L

E

Q

H

A

F

L

P

S

H

A

T

M

A

L

R

Y

K

R

N

G

F

H

D
|
D

I

V

T

L

L

F

I

I

V

C

M
x
Y

D

D

N
|
N

R

E

M
x
S

Y
|
Y

A
|
A

L
x
N

T
|
T

K

G

N

I

Q

Q

T

T

S

S

P

P

T

T

S

T

R

P

H

Y

G

G

Y

A

K

N

G

T

S

T

L

F

N

T

P

P

Y

P

G

G

N

E

I

V

E

V

D

-

P

P

F

E

N

G

V

K

I

K

K

L

F

F

-

P

-

K

-

D

-

N

-

P

-

K

A

V

I

I

A

A

S

H

G

G

-

H

-

P

-

E

A

L

T

K

F

Y

V

V

A

A

Q

T

S

A

Y

S

S

I

G

G

N

W

P

P

K

V

H

D

L

L

A

M

E

N

T

K

I

V

Q

R

A

K

A

G

V
x
L

E

N

H
x
Q

K

E

G

G

F

P

S

A

F

Y

V

I

N

H

V

I

L

H

S

A

P

P

C
|
C

P
|
P

active site: N143 (≠ L133)
binding [2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-carboxy-4-methyl-1,3-thiazol-3-ium-5-yl]ethoxy-oxidanyl-phosphoryl] hydrogen phosphate: T50 (vs. gap), G51 (vs. gap), C52 (vs. gap), I74 (vs. gap), G109 (= G99), D110 (= D100), G111 (= G101), Y136 (≠ M126), N138 (= N128), S140 (≠ M130), Y141 (= Y131), A142 (= A132), N143 (≠ L133), T144 (= T134)
binding magnesium ion: D110 (= D100), D130 (= D120), N138 (= N128), S140 (≠ M130), L211 (≠ V193), Q213 (≠ H195)
binding iron/sulfur cluster: C24 (= C24), C27 (= C27), P29 (≠ D29), C52 (vs. gap), C225 (= C207), P226 (= P208)

Sites not aligning to the query:

binding iron/sulfur cluster: 227

5exdF Crystal structure of oxalate oxidoreductase from moorella thermoacetica bound with carboxy-di-oxido-methyl-tpp (coom-tpp) intermediate (see paper)
25% identity, 68% coverage: 1:208/308 of query aligns to 2:226/310 of 5exdF

query
sites
5exdF

M

L

E

D

Y

R

I

I

K

A

L

S

E

I

E

K

K

K

L

A

P

P

P

D

K

E

E

E

Y

Y

R

Y

S

V

D

P

I

G

E

H

P

R

T

T

W

-

C
|
C

P

A

G

G

C
|
C

G

G

D
x
P

F

A

G

L

V

T

V

Y

T

R

A

L

L

V

T

A

K

K

A

A

F

A

S

G

E

P

E

N

R

T

F

I

-

F

-

I

D

G

P

P

T
|
T

A
x
G

I
x
C

-

M

-

Y

T

V

M

A

T

N

S

T

G

S

I

Y

G

G

C

C

S

G

S

P

R

W

L

R

P

V

L

P

W

W

M

I

-

H

-

A

-

Q

-
x
I

-

T

N

N

A

G

F

G

G

A

I

V

H

A

T

S

A

G

H

I

G

E

R

A

A

A

L

Y

P

K

A

A

A

M

V

I

G

R

V

K

R

K

L

K

A

T

R

D

P

A

E

E

T

F

P

P

T

N

I

I

-

I

V

V

T

M

A

A

G

G

D
|
D

G
|
G

D

G

I

A

F

V

S

D

I

I

G

G

M

L

E

Q

H

A

F

L

P

S

H

A

T

M

A

L

R

Y

K

R

N

G

F

H

D

D

I

V

T

L

L

F

I

I

V

C

M
x
Y

D

D

N
|
N

R

E

M
x
S

Y
|
Y

A
|
A

L
x
N

T
|
T

K

G

N

I

Q

Q

T

T

S

S

P

P

T

T

S

T

R

P

H

Y

G

G

Y

A

K

N

G

T

S

T

L

F

N

T

P

P

Y

P

G

G

N

E

I

V

-

V

-

P

-

E

-

G

-

K

-

L

-

F

-

P

E

K

D

D

P

N

F

P

N

K

V

V

I

I

K

A

F

H

A

G

I

H

A

P

S

E

G

-

A

L

T

K

F

Y

V

V

A

A

Q

T

S

A

Y

S

S

I

G

G

N

W

P

P

K

V

H

D

L

L

A

M

E

N

T

K

I

V

Q

R

A

K

A

G

V

L

E

N

H

Q

K

E

G

G

F

P

S

A

F

Y

V

I

N

H

V

I

L

H

S

A

P

P

C
|
C

P

P

active site: N143 (≠ L133)
binding magnesium ion: D110 (= D100), N138 (= N128), S140 (≠ M130)
binding [2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-[1,1,2-tris(oxidanyl)-2-oxidanylidene-ethyl]-1,3-thiazol-3-ium-5-yl]ethoxy-oxidanyl-phosphoryl] hydrogen phosphate: T50 (= T48), G51 (≠ A49), C52 (≠ I50), I74 (vs. gap), D110 (= D100), G111 (= G101), Y136 (≠ M126), N138 (= N128), S140 (≠ M130), Y141 (= Y131), A142 (= A132), N143 (≠ L133), T144 (= T134)
binding iron/sulfur cluster: C24 (= C24), C27 (= C27), P29 (≠ D29), C52 (≠ I50), A142 (= A132), C225 (= C207)

Sites not aligning to the query:

binding iron/sulfur cluster: 227

5c4iC Structure of an oxalate oxidoreductase (see paper)
25% identity, 68% coverage: 1:208/308 of query aligns to 2:226/312 of 5c4iC

query
sites
5c4iC

M

L

E

D

Y

R

I

I

K

A

L

S

E

I

E

K

K

K

L

A

P

P

P

D

K

E

E

E

Y

Y

R

Y

S

V

D

P

I

G

E

H

P

R

T

T

W

-

C
|
C

P

A

G

G

C
|
C

G

G

D
x
P

F

-

G

-

V

-

T

-

A

A

L

L

T

T

K

Y

A

R

F

L

S

V

E

A

E

K

R

A

F

A

D

G

P

P

T

N

A

T

I

I

-

F

-

I

-

G

-

P

-
x
T

-
x
G

-
x
C

-

M

-

Y

T

V

M

A

T

N

S

T

G

S

I

Y

G

G

C

C

S

G

S

P

R

W

L

R

P

V

L

P

W

W

M

I

-

H

-

A

-

Q

-
x
I

-

T

N

N

A

G

F

G

G

A

I

V

H

A

T

S

A

G

H

I

G

E

R

A

A

A

L

Y

P

K

A

A

A

M

V

I

G

R

V

K

R

K

L

K

A

T

R

D

P

A

E

E

T

F

P

P

T

N

I

I

-

I

V

V

T

M

A

A

G
|
G

D
|
D

G
|
G

D

G

I

A

F

V

S

D

I

I

G

G

M

L

E

Q

H

A

F

L

P

S

H

A

T

M

A

L

R

Y

K

R

N

G

F

H

D

D

I

V

T

L

L

F

I

I

V

C

M
x
Y

D

D

N
|
N

R

E

M
x
S

Y
|
Y

A
|
A

L
x
N

T
|
T

K

G

N

I

Q

Q

T

T

S

S

P

P

T

T

S

T

R

P

H

Y

G

G

Y

A

K

N

G

T

S

T

L

F

N

T

P

P

Y

P

G

G

N

E

I

V

-

V

-

P

-

E

-

G

-

K

-

L

-

F

-

P

E

K

D

D

P

N

F

P

N

K

V

V

I

I

K

A

F

H

A

G

I

H

A

P

S

E

G

-

A

L

T

K

F

Y

V

V

A

A

Q

T

S

A

Y

S

S

I

G

G

N

W

P

P

K

V

H

D

L

L

A

M

E

N

T

K

I

V

Q

R

A

K

A

G

V

L

E

N

H

Q

K

E

G

G

F

P

S

A

F

Y

V

I

N

H

V

I

L

H

S

A

P

P

C
|
C

P
|
P

active site: N143 (≠ L133)
binding magnesium ion: D110 (= D100), N138 (= N128), S140 (≠ M130)
binding iron/sulfur cluster: C24 (= C24), C27 (= C27), P29 (≠ D29), C52 (vs. gap), A142 (= A132), C225 (= C207), P226 (= P208)
binding thiamine diphosphate: T50 (vs. gap), G51 (vs. gap), C52 (vs. gap), I74 (vs. gap), G109 (= G99), D110 (= D100), G111 (= G101), Y136 (≠ M126), N138 (= N128), S140 (≠ M130), Y141 (= Y131), A142 (= A132), N143 (≠ L133), T144 (= T134)

Sites not aligning to the query:

binding iron/sulfur cluster: 227

6lpiB Crystal structure of ahas holo-enzyme (see paper)
28% identity, 28% coverage: 65:151/308 of query aligns to 394:483/539 of 6lpiB

query
sites
6lpiB

W

W

M

L

N

T

A

S

F
x
G

G

G

I

L

H
x
G

T

T

A
x
M

H

-

G

G

R

F

A

G

L

L

P

P

A

A

V

I

G

G

V

A

R

A

L

L

A

A

R

N

P

P

E

D

T

R

P

K

T

V

I

L

V

C

T

F

A

S

G
|
G

D
|
D

G
|
G

D
x
S

I

L

F

M

S

-

I
x
M

G

N

M

I

E

Q

H

E

F

M

P

A

H

T

T

A

A

S

R

E

K

N

N

Q

F

L

D

D

I

V

T

K

L

I

I

I

V

L

M

M

D

N

N
|
N

R

E

M
x
A

Y
x
L

A
x
G

L
|
L

T
x
V

K

H

N

Q

Q
|
Q

T

Q

S

S

P

L

T

F

S

Y

R

E

H

Q

G

G

-

V

-

F

-

A

-

T

Y

Y

K

P

G

G

S

K

L

I

N

N

active site: G401 (≠ H72), M403 (≠ A74), D428 (= D100), N455 (= N128), A457 (≠ M130), L458 (≠ Y131), L460 (= L133), V461 (≠ T134), Q464 (= Q137)
binding flavin-adenine dinucleotide: G398 (≠ F69)
binding magnesium ion: D428 (= D100), N455 (= N128)
binding thiamine diphosphate: G401 (≠ H72), M403 (≠ A74), G427 (= G99), D428 (= D100), G429 (= G101), S430 (≠ D102), M433 (≠ I106), N455 (= N128), A457 (≠ M130), L458 (≠ Y131), G459 (≠ A132), L460 (= L133), V461 (≠ T134)

Sites not aligning to the query:

active site: 27, 29, 30, 31, 32, 53, 76, 115, 116, 117, 165, 256, 283, 375
binding flavin-adenine dinucleotide: 155, 212, 213, 214, 236, 237, 238, 254, 256, 257, 276, 277, 278, 280, 282, 283, 300, 301, 319, 320, 380
binding thiamine diphosphate: 53, 76, 79, 376, 377, 378

Q2RMD6 Pyruvate:ferredoxin oxidoreductase; PFOR; Pyruvate synthase; EC 1.2.7.1 from Moorella thermoacetica (strain ATCC 39073 / JCM 9320) (see paper)
22% identity, 65% coverage: 96:296/308 of query aligns to 963:1149/1171 of Q2RMD6

query
sites
Q2RMD6

V

I

T

I

A

G

G

G

D
|
D

G
|
G

D
x
W

I

A

F

Y

S

D

I

I

G

G

M

Y

E

G

H

G

F

L

P

D

H

H

T

V

A

L

R

A

K

S

N

G

F

A

D

N

I

V

T

N

L

V

I

L

V

V

M

L

D

D

N
x
T

R
x
E

M
x
V

Y
|
Y

A
x
S

L
x
N

T

T

K

G

N

G

Q

Q

T

S

S

S

P

K

T

A

S

T

R

Q

H

T

G

G

Y

A

K

V

G

A

S

R

L

F

N

A

P

A

Y

G

G

G

N

K

I

F

E

T

D

K

P

K

F

K

N

D

V

L

I

G

K

L

F

M

A

A

I

M

A

S

S

Y

G

G

A

Y

T

V

F

Y

V

V

A

A

Q

S

S

V

Y

A

S

M

G

G

-

A

N

S

P

H

K

S

H

Q

L

L

A

M

E

K

T

A

I

L

Q

I

A

E

A

A

V

E

E

K

H

Y

K

D

G

G

F

P

S

S

F

L

V

I

N

I

V

A

L

Y

S

A

P

P

C
|
C

P

-

T

-

F

-

N

-

K

-

I

-

D

-

T

-

F

-

K

-

Y

-

K

-

G

-

R

-

L

-

I

I

D

N

I

H

N

G

K

I

E

N

L

M

G

T

H

Y

D

S

P

Q

S

R

D

E

M

A

K

K

A

K

A

A

L

V

E

E

L

A

A

G

S

Y

H

W

V

P

L

L

D

Y

A

R

D

Y

Y

N

P

P

E

Q

L

L

E

A

D

Q

A

-

E

-

P

-

F

-

K

E

G

G

K

K

R

N

P

P

I

F

G

I

I

L

F

D

Y

Y

K

K

V

T

E

P

K

T

E

A

T

S

F

F

-

R

-

D

-

F

-

L

-

M

-

G

E

E

E

I

R

R

I

Y

A

T

K

S

L

L

K

K

E

K

K

Q

F

F

A

-

P

P

K

E

E

K

G

A

E

E

E

Q

D967 (= D100) binding Mg(2+)
DGW 967:969 (≠ DGD 100:102) binding thiamine diphosphate
T995 (≠ N128) binding Mg(2+)
TEVYSN 995:1000 (≠ NRMYAL 128:133) binding thiamine diphosphate
V997 (≠ M130) binding Mg(2+)
C1075 (= C207) binding [4Fe-4S] cluster

Sites not aligning to the query:

424:428 binding CoA
456 binding CoA
556 binding CoA
598 binding CoA
686 binding [4Fe-4S] cluster
689 binding [4Fe-4S] cluster
692 binding [4Fe-4S] cluster
696 binding [4Fe-4S] cluster
742 binding [4Fe-4S] cluster
745 binding [4Fe-4S] cluster
748 binding [4Fe-4S] cluster
752 binding [4Fe-4S] cluster
809 binding [4Fe-4S] cluster
812 binding [4Fe-4S] cluster
814 binding thiamine diphosphate
837 binding [4Fe-4S] cluster; binding thiamine diphosphate

6ciqA Pyruvate:ferredoxin oxidoreductase from moorella thermoacetica with coenzyme a bound (see paper)
22% identity, 65% coverage: 96:296/308 of query aligns to 962:1148/1169 of 6ciqA

query
sites
6ciqA

V

I

T

I

A

G

G
|
G

D
|
D

G
|
G

D

W

I

A

F

Y

S

D

I

I

G

G

M

Y

E

G

H

G

F

L

P

D

H

H

T

V

A

L

R

A

K

S

N

G

F

A

D

N

I

V

T

N

L

V

I

L

V

V

M

L

D

D

N
x
T

R

E

M
x
V

Y
|
Y

A
x
S

L
x
N

T
|
T

K

G

N

G

Q

Q

T

S

S

S

P

K

T

A

S

T

R

Q

H

T

G

G

Y

A

K

V

G

A

S

R

L

F

N

A

P

A

Y

G

G

G

N

K

I

F

E

T

D

K

P

K

F

K

N

D

V

L

I

G

K

L

F

M

A

A

I

M

A

S

S

Y

G

G

A

Y

T

V

F

Y

V

V

A

A

Q

S

S

V

Y

A

S

M

G

G

-

A

N

S

P

H

K

S

H

Q

L

L

A

M

E

K

T

A

I

L

Q

I

A

E

A

A

V

E

E

K

H

Y

K

D

G

G

F

P

S

S

F

L

V

I

N

I

V

A

L

Y

S

A

P

P

C
|
C

P

-

T

-

F

-

N

-

K

-

I

-

D

-

T

-

F

-

K

-

Y

-

K

-

G

-

R

-

L

-

I
|
I

D

N

I

H

N

G

K

I

E

N

L

M

G

T

H

Y

D

S

P

Q

S

R

D

E

M

A

K

K

A

K

A

A

L

V

E

E

L

A

A

G

S

Y

H

W

V

P

L

L

D

Y

A

R

D

Y

Y

N

P

P

E

Q

L

L

E

A

D

Q

A

-

E

-

P

-

F

-

K

E

G

G

K

K

R

N

P

P

I

F

G

I

I

L

F

D

Y

Y

K

K

V

T

E

P

K

T

E

A

T

S

F

F

-

R

-

D

-

F

-

L

-

M

-

G

E

E

E

I

R

R

I

Y

A

T

K

S

L

L

K

K

E

K

K

Q

F

F

A

-

P

P

K

E

E

K

G

A

E

E

E

Q

active site: N999 (≠ L133)
binding coenzyme a: N999 (≠ L133)
binding magnesium ion: D966 (= D100), T994 (≠ N128), V996 (≠ M130)
binding iron/sulfur cluster: S998 (≠ A132), C1074 (= C207), I1075 (= I224)
binding thiamine diphosphate: G965 (= G99), D966 (= D100), G967 (= G101), V996 (≠ M130), Y997 (= Y131), S998 (≠ A132), N999 (≠ L133), T1000 (= T134)

Sites not aligning to the query:

active site: 28, 61, 111
binding coenzyme a: 28, 423, 424, 427, 428, 553, 586
binding iron/sulfur cluster: 678, 680, 685, 686, 688, 689, 691, 695, 696, 700, 734, 741, 742, 743, 744, 745, 747, 751, 757, 807, 808, 811, 813, 836
binding thiamine diphosphate: 26, 27, 61, 85, 813, 835, 836, 865

6cinB Crystal structure of pyruvate:ferredoxin oxidoreductase from moorella thermoacetica (see paper)
22% identity, 65% coverage: 96:296/308 of query aligns to 962:1148/1169 of 6cinB

query
sites
6cinB

V

I

T

I

A

G

G
|
G

D
|
D

G
|
G

D

W

I

A

F

Y

S

D

I

I

G

G

M

Y

E

G

H

G

F

L

P

D

H

H

T

V

A

L

R

A

K

S

N

G

F

A

D

N

I

V

T

N

L

V

I

L

V

V

M

L

D

D

N
x
T

R

E

M
x
V

Y
|
Y

A
x
S

L
x
N

T
|
T

K

G

N

G

Q

Q

T

S

S

S

P

K

T

A

S

T

R

Q

H

T

G

G

Y

A

K

V

G

A

S

R

L

F

N

A

P

A

Y

G

G

G

N

K

I

F

E

T

D

K

P

K

F

K

N

D

V

L

I

G

K

L

F

M

A

A

I

M

A

S

S

Y

G

G

A

Y

T

V

F

Y

V

V

A

A

Q

S

S

V

Y

A

S

M

G

G

-

A

N

S

P

H

K

S

H

Q

L

L

A

M

E

K

T

A

I

L

Q

I

A

E

A

A

V

E

E

K

H

Y

K

D

G

G

F

P

S

S

F

L

V

I

N

I

V

A

L

Y

S

A

P

P

C
|
C

P

-

T

-

F

-

N

-

K

-

I

-

D

-

T

-

F

-

K

-

Y

-

K

-

G

-

R

-

L

-

I
|
I

D

N

I

H

N

G

K

I

E

N

L

M

G

T

H

Y

D

S

P

Q

S

R

D

E

M

A

K

K

A

K

A

A

L

V

E

E

L

A

A

G

S

Y

H

W

V

P

L

L

D

Y

A

R

D

Y

Y

N

P

P

E

Q

L

L

E

A

D

Q

A

-

E

-

P

-

F

-

K

E

G

G

K

K

R

N

P

P

I

F

G

I

I

L

F

D

Y

Y

K

K

V

T

E

P

K

T

E

A

T

S

F

F

-

R

-

D

-

F

-

L

-

M

-

G

E

E

E

I

R

R

I

Y

A

T

K

S

L

L

K

K

E

K

K

Q

F

F

A

-

P

P

K

E

E

K

G

A

E

E

E

Q

active site: N999 (≠ L133)
binding magnesium ion: D966 (= D100), T994 (≠ N128), V996 (≠ M130)
binding iron/sulfur cluster: S998 (≠ A132), C1074 (= C207), I1075 (= I224)
binding thiamine diphosphate: G965 (= G99), D966 (= D100), G967 (= G101), V996 (≠ M130), Y997 (= Y131), S998 (≠ A132), N999 (≠ L133), T1000 (= T134)

Sites not aligning to the query:

active site: 28, 61, 111
binding iron/sulfur cluster: 455, 678, 685, 686, 687, 688, 689, 691, 695, 700, 741, 742, 743, 744, 745, 746, 747, 751, 752, 757, 758, 807, 808, 811, 813, 836
binding thiamine diphosphate: 26, 27, 61, 834, 835, 836, 865

Query Sequence

>WP_012676275.1 NCBI__GCF_000021565.1:WP_012676275.1
MEYIKLEEKLPPKEYRSDIEPTWCPGCGDFGVVTALTKAFSEERFDPTAITMTSGIGCSS
RLPLWMNAFGIHTAHGRALPAAVGVRLARPETPTIVTAGDGDIFSIGMEHFPHTARKNFD
ITLIVMDNRMYALTKNQTSPTSRHGYKGSLNPYGNIEDPFNVIKFAIASGATFVAQSYSG
NPKHLAETIQAAVEHKGFSFVNVLSPCPTFNKIDTFKYYKGRLIDINKELGHDPSDMKAA
LELASHVLDADYPELEDAEPFKGKRPIGIFYKVEKETFEERIAKLKEKFAPKEGEEPDWD
EILAKYRP

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory