SitesBLAST
Comparing WP_012676275.1 NCBI__GCF_000021565.1:WP_012676275.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 16 hits to proteins with known functional sites (download)
6n2oD 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus with 2-oxoglutarate, coenzyme a and succinyl-coa bound (see paper)
43% identity, 98% coverage: 8:308/308 of query aligns to 9:291/291 of 6n2oD
- binding magnesium ion: D101 (= D100), N129 (= N128), I131 (≠ M130)
- binding succinyl-coenzyme a: I57 (= I56), R62 (= R61), L134 (= L133), K136 (= K135)
- binding iron/sulfur cluster: W24 (= W23), C25 (= C24), C28 (= C27), C59 (= C58), C208 (= C207), T210 (= T209), F211 (= F210)
- binding thiamine diphosphate: I57 (= I56), G58 (= G57), C59 (= C58), S60 (= S59), H76 (= H75), G102 (= G101), D103 (= D102), N129 (= N128), I131 (≠ M130), G133 (≠ A132), L134 (= L133), T135 (= T134)
6n2oB 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus with 2-oxoglutarate, coenzyme a and succinyl-coa bound (see paper)
43% identity, 98% coverage: 8:308/308 of query aligns to 9:291/291 of 6n2oB
- binding 2-oxoglutaric acid: R62 (= R61), L134 (= L133)
- binding coenzyme a: K136 (= K135), Y150 (≠ S149)
- binding magnesium ion: D101 (= D100), N129 (= N128), I131 (≠ M130)
- binding iron/sulfur cluster: W24 (= W23), C25 (= C24), C28 (= C27), C59 (= C58), C208 (= C207), T210 (= T209), F211 (= F210)
- binding thiamine diphosphate: I57 (= I56), G58 (= G57), C59 (= C58), S60 (= S59), H76 (= H75), G102 (= G101), D103 (= D102), N129 (= N128), I131 (≠ M130), Y132 (= Y131), G133 (≠ A132), L134 (= L133), T135 (= T134)
6n2nB Crystal structure of 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus (see paper)
43% identity, 98% coverage: 8:308/308 of query aligns to 9:291/291 of 6n2nB
- binding magnesium ion: D101 (= D100), N129 (= N128), I131 (≠ M130)
- binding iron/sulfur cluster: W24 (= W23), C25 (= C24), C28 (= C27), H30 (≠ D29), C59 (= C58), C208 (= C207), T210 (= T209), F211 (= F210)
- binding thiamine diphosphate: I57 (= I56), G58 (= G57), C59 (= C58), S60 (= S59), H76 (= H75), G100 (= G99), D101 (= D100), G102 (= G101), D103 (= D102), N129 (= N128), I131 (≠ M130), Y132 (= Y131), G133 (≠ A132), L134 (= L133), T135 (= T134)
Q96XT4 2-oxoacid:ferredoxin oxidoreductase 2, subunit beta; OFOR2; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
43% identity, 84% coverage: 23:282/308 of query aligns to 11:268/304 of Q96XT4
- C12 (= C24) binding [4Fe-4S] cluster
- C15 (= C27) binding [4Fe-4S] cluster
- IGCS 44:47 (= IGCS 56:59) binding thiamine diphosphate
- C46 (= C58) binding [4Fe-4S] cluster
- D90 (= D100) binding Mg(2+)
- GD 91:92 (= GD 101:102) binding thiamine diphosphate
- N118 (= N128) binding Mg(2+)
- V120 (≠ M130) binding Mg(2+)
- GL 122:123 (≠ AL 132:133) binding thiamine diphosphate
- C197 (= C207) binding [4Fe-4S] cluster
5b46B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - ligand free form (see paper)
43% identity, 84% coverage: 23:282/308 of query aligns to 8:265/301 of 5b46B
- binding magnesium ion: D87 (= D100), N115 (= N128), V117 (≠ M130)
- binding iron/sulfur cluster: W8 (= W23), C9 (= C24), C12 (= C27), C43 (= C58), C194 (= C207), T196 (= T209), Y197 (≠ F210)
- binding thiamine diphosphate: I41 (= I56), G42 (= G57), C43 (= C58), S44 (= S59), H62 (= H75), G86 (= G99), G88 (= G101), D89 (= D102), N115 (= N128), V117 (≠ M130), Y118 (= Y131), G119 (≠ A132), L120 (= L133), T121 (= T134)
Q96Y68 2-oxoacid:ferredoxin oxidoreductase 1, subunit beta; OFOR1; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 2 papers)
41% identity, 84% coverage: 23:282/308 of query aligns to 11:268/305 of Q96Y68
- C12 (= C24) binding [4Fe-4S] cluster
- C15 (= C27) binding [4Fe-4S] cluster
- C46 (= C58) binding [4Fe-4S] cluster
- K49 (≠ R61) mutation to I: Loss of oxidoreductase activity toward 2-oxoglutarate but retains its activity toward pyruvate.
- D90 (= D100) binding Mg(2+)
- GD 91:92 (= GD 101:102) binding thiamine diphosphate
- N118 (= N128) binding Mg(2+)
- V120 (≠ M130) binding Mg(2+)
- GL 122:123 (≠ AL 132:133) binding thiamine diphosphate
- K125 (= K135) Plays an important role in the binding of CoA; mutation to A: Shows a strong decrease of affinity for CoA and a poor inactivation by 4-fluoro-7-nitrobenzofurazan (NBD-F).
- K173 (= K183) mutation to A: Same oxidoreductase activity as the wild-type.
- C197 (= C207) binding [4Fe-4S] cluster
P72579 2-oxoacid:ferredoxin oxidoreductase subunit beta; OFOR; EC 1.2.7.11 from Sulfolobus sp. (see paper)
41% identity, 84% coverage: 23:282/308 of query aligns to 11:268/305 of P72579
- K49 (≠ R61) mutation to I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to R: Increase the oxidoreductase activity with pyruvate.; mutation to V: Slight decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.
- L123 (= L133) mutation L->A,I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to N: Strong decrease of the oxidoreductase activity with pyruvate and 2-oxobutyrate. However, this mutant shows almost the same activity with 2-oxoglutarate as the wild-type.
5b48B 2-oxoacid:ferredoxin oxidoreductase 1 from sulfolobus tokodai (see paper)
41% identity, 84% coverage: 23:282/308 of query aligns to 7:250/286 of 5b48B
- binding magnesium ion: D84 (= D100), N112 (= N128), V114 (≠ M130)
- binding iron/sulfur cluster: C8 (= C24), C11 (= C27), C42 (= C58), C183 (= C207), T185 (= T209)
- binding 2-[(2E)-3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-(1-oxidanylpropylidene)-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: I40 (= I56), G41 (= G57), C42 (= C58), S43 (= S59), H59 (= H75), G85 (= G101), D86 (= D102), N112 (= N128), V114 (≠ M130), Y115 (= Y131), G116 (≠ A132), L117 (= L133)
5b47B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - pyruvate complex (see paper)
40% identity, 84% coverage: 23:282/308 of query aligns to 4:239/275 of 5b47B
- binding magnesium ion: D83 (= D100), N111 (= N128)
- binding iron/sulfur cluster: C5 (= C24), C8 (= C27), C39 (= C58), C179 (= C207)
- binding thiamine diphosphate: I37 (= I56), G38 (= G57), C39 (= C58), S40 (= S59), H58 (= H75), G84 (= G101), D85 (= D102), N111 (= N128), V113 (≠ M130), Y114 (= Y131), L116 (= L133)
5exeC Crystal structure of oxalate oxidoreductase from moorella thermoacetica bound with carboxy-tpp adduct (see paper)
26% identity, 68% coverage: 1:208/308 of query aligns to 2:226/314 of 5exeC
- active site: N143 (≠ L133)
- binding [2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-carboxy-4-methyl-1,3-thiazol-3-ium-5-yl]ethoxy-oxidanyl-phosphoryl] hydrogen phosphate: T50 (vs. gap), G51 (vs. gap), C52 (vs. gap), I74 (vs. gap), G109 (= G99), D110 (= D100), G111 (= G101), Y136 (≠ M126), N138 (= N128), S140 (≠ M130), Y141 (= Y131), A142 (= A132), N143 (≠ L133), T144 (= T134)
- binding magnesium ion: D110 (= D100), D130 (= D120), N138 (= N128), S140 (≠ M130), L211 (≠ V193), Q213 (≠ H195)
- binding iron/sulfur cluster: C24 (= C24), C27 (= C27), P29 (≠ D29), C52 (vs. gap), C225 (= C207), P226 (= P208)
Sites not aligning to the query:
5exdF Crystal structure of oxalate oxidoreductase from moorella thermoacetica bound with carboxy-di-oxido-methyl-tpp (coom-tpp) intermediate (see paper)
25% identity, 68% coverage: 1:208/308 of query aligns to 2:226/310 of 5exdF
- active site: N143 (≠ L133)
- binding magnesium ion: D110 (= D100), N138 (= N128), S140 (≠ M130)
- binding [2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-[1,1,2-tris(oxidanyl)-2-oxidanylidene-ethyl]-1,3-thiazol-3-ium-5-yl]ethoxy-oxidanyl-phosphoryl] hydrogen phosphate: T50 (= T48), G51 (≠ A49), C52 (≠ I50), I74 (vs. gap), D110 (= D100), G111 (= G101), Y136 (≠ M126), N138 (= N128), S140 (≠ M130), Y141 (= Y131), A142 (= A132), N143 (≠ L133), T144 (= T134)
- binding iron/sulfur cluster: C24 (= C24), C27 (= C27), P29 (≠ D29), C52 (≠ I50), A142 (= A132), C225 (= C207)
Sites not aligning to the query:
5c4iC Structure of an oxalate oxidoreductase (see paper)
25% identity, 68% coverage: 1:208/308 of query aligns to 2:226/312 of 5c4iC
- active site: N143 (≠ L133)
- binding magnesium ion: D110 (= D100), N138 (= N128), S140 (≠ M130)
- binding iron/sulfur cluster: C24 (= C24), C27 (= C27), P29 (≠ D29), C52 (vs. gap), A142 (= A132), C225 (= C207), P226 (= P208)
- binding thiamine diphosphate: T50 (vs. gap), G51 (vs. gap), C52 (vs. gap), I74 (vs. gap), G109 (= G99), D110 (= D100), G111 (= G101), Y136 (≠ M126), N138 (= N128), S140 (≠ M130), Y141 (= Y131), A142 (= A132), N143 (≠ L133), T144 (= T134)
Sites not aligning to the query:
6lpiB Crystal structure of ahas holo-enzyme (see paper)
28% identity, 28% coverage: 65:151/308 of query aligns to 394:483/539 of 6lpiB
- active site: G401 (≠ H72), M403 (≠ A74), D428 (= D100), N455 (= N128), A457 (≠ M130), L458 (≠ Y131), L460 (= L133), V461 (≠ T134), Q464 (= Q137)
- binding flavin-adenine dinucleotide: G398 (≠ F69)
- binding magnesium ion: D428 (= D100), N455 (= N128)
- binding thiamine diphosphate: G401 (≠ H72), M403 (≠ A74), G427 (= G99), D428 (= D100), G429 (= G101), S430 (≠ D102), M433 (≠ I106), N455 (= N128), A457 (≠ M130), L458 (≠ Y131), G459 (≠ A132), L460 (= L133), V461 (≠ T134)
Sites not aligning to the query:
- active site: 27, 29, 30, 31, 32, 53, 76, 115, 116, 117, 165, 256, 283, 375
- binding flavin-adenine dinucleotide: 155, 212, 213, 214, 236, 237, 238, 254, 256, 257, 276, 277, 278, 280, 282, 283, 300, 301, 319, 320, 380
- binding thiamine diphosphate: 53, 76, 79, 376, 377, 378
Q2RMD6 Pyruvate:ferredoxin oxidoreductase; PFOR; Pyruvate synthase; EC 1.2.7.1 from Moorella thermoacetica (strain ATCC 39073 / JCM 9320) (see paper)
22% identity, 65% coverage: 96:296/308 of query aligns to 963:1149/1171 of Q2RMD6
- D967 (= D100) binding Mg(2+)
- DGW 967:969 (≠ DGD 100:102) binding thiamine diphosphate
- T995 (≠ N128) binding Mg(2+)
- TEVYSN 995:1000 (≠ NRMYAL 128:133) binding thiamine diphosphate
- V997 (≠ M130) binding Mg(2+)
- C1075 (= C207) binding [4Fe-4S] cluster
Sites not aligning to the query:
- 424:428 binding CoA
- 456 binding CoA
- 556 binding CoA
- 598 binding CoA
- 686 binding [4Fe-4S] cluster
- 689 binding [4Fe-4S] cluster
- 692 binding [4Fe-4S] cluster
- 696 binding [4Fe-4S] cluster
- 742 binding [4Fe-4S] cluster
- 745 binding [4Fe-4S] cluster
- 748 binding [4Fe-4S] cluster
- 752 binding [4Fe-4S] cluster
- 809 binding [4Fe-4S] cluster
- 812 binding [4Fe-4S] cluster
- 814 binding thiamine diphosphate
- 837 binding [4Fe-4S] cluster; binding thiamine diphosphate
6ciqA Pyruvate:ferredoxin oxidoreductase from moorella thermoacetica with coenzyme a bound (see paper)
22% identity, 65% coverage: 96:296/308 of query aligns to 962:1148/1169 of 6ciqA
- active site: N999 (≠ L133)
- binding coenzyme a: N999 (≠ L133)
- binding magnesium ion: D966 (= D100), T994 (≠ N128), V996 (≠ M130)
- binding iron/sulfur cluster: S998 (≠ A132), C1074 (= C207), I1075 (= I224)
- binding thiamine diphosphate: G965 (= G99), D966 (= D100), G967 (= G101), V996 (≠ M130), Y997 (= Y131), S998 (≠ A132), N999 (≠ L133), T1000 (= T134)
Sites not aligning to the query:
- active site: 28, 61, 111
- binding coenzyme a: 28, 423, 424, 427, 428, 553, 586
- binding iron/sulfur cluster: 678, 680, 685, 686, 688, 689, 691, 695, 696, 700, 734, 741, 742, 743, 744, 745, 747, 751, 757, 807, 808, 811, 813, 836
- binding thiamine diphosphate: 26, 27, 61, 85, 813, 835, 836, 865
6cinB Crystal structure of pyruvate:ferredoxin oxidoreductase from moorella thermoacetica (see paper)
22% identity, 65% coverage: 96:296/308 of query aligns to 962:1148/1169 of 6cinB
- active site: N999 (≠ L133)
- binding magnesium ion: D966 (= D100), T994 (≠ N128), V996 (≠ M130)
- binding iron/sulfur cluster: S998 (≠ A132), C1074 (= C207), I1075 (= I224)
- binding thiamine diphosphate: G965 (= G99), D966 (= D100), G967 (= G101), V996 (≠ M130), Y997 (= Y131), S998 (≠ A132), N999 (≠ L133), T1000 (= T134)
Sites not aligning to the query:
- active site: 28, 61, 111
- binding iron/sulfur cluster: 455, 678, 685, 686, 687, 688, 689, 691, 695, 700, 741, 742, 743, 744, 745, 746, 747, 751, 752, 757, 758, 807, 808, 811, 813, 836
- binding thiamine diphosphate: 26, 27, 61, 834, 835, 836, 865
Query Sequence
>WP_012676275.1 NCBI__GCF_000021565.1:WP_012676275.1
MEYIKLEEKLPPKEYRSDIEPTWCPGCGDFGVVTALTKAFSEERFDPTAITMTSGIGCSS
RLPLWMNAFGIHTAHGRALPAAVGVRLARPETPTIVTAGDGDIFSIGMEHFPHTARKNFD
ITLIVMDNRMYALTKNQTSPTSRHGYKGSLNPYGNIEDPFNVIKFAIASGATFVAQSYSG
NPKHLAETIQAAVEHKGFSFVNVLSPCPTFNKIDTFKYYKGRLIDINKELGHDPSDMKAA
LELASHVLDADYPELEDAEPFKGKRPIGIFYKVEKETFEERIAKLKEKFAPKEGEEPDWD
EILAKYRP
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory