SitesBLAST
Comparing WP_012676469.1 NCBI__GCF_000021565.1:WP_012676469.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
8pnyA Crystal structure of d-amino acid aminotransferase from blastococcus saxobsidens complexed with phenylhydrazine and in its apo form (see paper)
25% identity, 98% coverage: 5:247/249 of query aligns to 13:260/278 of 8pnyA
- binding [6-methyl-5-oxidanyl-4-[(2-phenylhydrazinyl)methyl]pyridin-3-yl]methyl dihydrogen phosphate: F36 (= F28), S38 (≠ T30), R55 (= R47), K152 (= K139), Y156 (= Y143), E185 (= E172), G186 (≠ T173), P187 (≠ S174), T188 (≠ S175), S189 (≠ A176), T190 (≠ N177), L209 (= L196), T212 (≠ V199), T213 (= T200), S248 (≠ N235), G249 (≠ A236)
8pnxA Crystal structure of d-amino acid aminotransferase from blastococcus saxobsidens in pmp form
25% identity, 98% coverage: 5:247/249 of query aligns to 13:260/278 of 8pnxA
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R55 (= R47), K152 (= K139), Y156 (= Y143), E185 (= E172), T188 (≠ S175), S189 (≠ A176), T190 (≠ N177), L209 (= L196), T212 (≠ V199), T213 (= T200), S248 (≠ N235), G249 (≠ A236)
8pnwA Crystal structure of d-amino acid aminotransferase from blastococcus saxobsidens in holo form with plp (see paper)
25% identity, 98% coverage: 5:247/249 of query aligns to 13:260/278 of 8pnwA
- binding pyridoxal-5'-phosphate: R55 (= R47), K152 (= K139), Y156 (= Y143), E185 (= E172), P187 (≠ S174), T188 (≠ S175), S189 (≠ A176), L209 (= L196), T212 (≠ V199), T213 (= T200), G249 (≠ A236)
5mr0D Thermophilic archaeal branched-chain amino acid transaminases from geoglobus acetivorans and archaeoglobus fulgidus: biochemical and structural characterisation (see paper)
35% identity, 88% coverage: 17:234/249 of query aligns to 21:244/290 of 5mr0D
- active site: F32 (= F28), G34 (≠ T30), K150 (= K139), E183 (= E172), L206 (= L196)
- binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: R51 (≠ Q43), G100 (≠ Y95), L101 (≠ F96), K150 (= K139), Y154 (= Y143), E183 (= E172), G186 (≠ S175), D187 (≠ A176), L206 (= L196), I209 (≠ V199), T210 (= T200)
Sites not aligning to the query:
5e25A Crystal structure of branched-chain aminotransferase from thermophilic archaea geoglobus acetivorans complexed with alpha-ketoglutarate (see paper)
33% identity, 93% coverage: 17:247/249 of query aligns to 22:258/290 of 5e25A
- active site: F33 (= F28), G35 (≠ T30), K151 (= K139), E184 (= E172), L207 (= L196)
- binding 2-oxoglutaric acid: Y88 (≠ L82), K151 (= K139), T247 (≠ A236), A248 (≠ L237)
- binding pyridoxal-5'-phosphate: R52 (≠ Q43), K151 (= K139), Y155 (= Y143), E184 (= E172), G187 (≠ S175), D188 (≠ A176), L207 (= L196), G209 (= G198), I210 (≠ V199), T211 (= T200), G246 (≠ N235), T247 (≠ A236)
6thqB Crystal structure of branched-chain aminotransferase from thermophilic archaea thermoproteus uzoniensis with norvaline
32% identity, 86% coverage: 20:232/249 of query aligns to 29:250/301 of 6thqB
- active site: F37 (= F28), K156 (= K139), E190 (= E172), L214 (= L196)
- binding pyridoxal-5'-phosphate: R60 (= R47), K156 (= K139), Y161 (= Y143), E190 (= E172), N195 (= N177), L214 (= L196), G216 (= G198), I217 (≠ V199), T218 (= T200)
- binding 2-[o-phosphonopyridoxyl]-amino-pentanoic acid: R60 (= R47), Y97 (= Y83), K156 (= K139), Y161 (= Y143), E190 (= E172), G193 (≠ S175), E194 (≠ A176), N195 (= N177), G216 (= G198), I217 (≠ V199), T218 (= T200)
Sites not aligning to the query:
1i2lA Deoxychorismate lyase from escherichia coli with inhibitor
26% identity, 92% coverage: 19:246/249 of query aligns to 17:247/269 of 1i2lA
- active site: F26 (= F28), T28 (= T30), I139 (= I138), K140 (= K139), E173 (= E172), V197 (≠ L196)
- binding d-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-n,o-cycloserylamide: R45 (= R47), K140 (= K139), E173 (= E172), C175 (≠ S174), A176 (≠ S175), A177 (= A176), N178 (= N177), V197 (≠ L196), G199 (= G198), I200 (≠ V199), M201 (≠ T200), N236 (= N235), A237 (= A236)
1i2kA Aminodeoxychorismate lyase from escherichia coli
26% identity, 92% coverage: 19:246/249 of query aligns to 17:247/269 of 1i2kA
- active site: F26 (= F28), T28 (= T30), I139 (= I138), K140 (= K139), E173 (= E172), V197 (≠ L196)
- binding pyridoxal-5'-phosphate: R45 (= R47), K140 (= K139), E173 (= E172), A176 (≠ S175), A177 (= A176), V197 (≠ L196), G199 (= G198), I200 (≠ V199), M201 (≠ T200), N236 (= N235), A237 (= A236)
7p3tB Transaminase of gamma-proteobacterium (see paper)
28% identity, 92% coverage: 19:246/249 of query aligns to 25:258/299 of 7p3tB
- binding pyridoxal-5'-phosphate: R53 (= R47), K153 (= K137), R157 (≠ T141), E186 (= E172), S187 (≠ T173), A188 (≠ S174), A189 (≠ S175), S190 (≠ A176), G210 (= G198), I211 (≠ V199), T212 (= T200), T248 (≠ A236)
4whxA X-ray crystal structure of an amino acid aminotransferase from burkholderia pseudomallei bound to the co-factor pyridoxal phosphate
26% identity, 89% coverage: 11:232/249 of query aligns to 14:253/306 of 4whxA
Sites not aligning to the query:
P19938 D-alanine aminotransferase; D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase; EC 2.6.1.21 from Bacillus sp. (strain YM-1) (see 5 papers)
30% identity, 92% coverage: 19:247/249 of query aligns to 23:253/283 of P19938
- Y32 (≠ F28) binding substrate
- R51 (= R47) binding pyridoxal 5'-phosphate
- R99 (vs. gap) binding substrate
- H101 (vs. gap) binding substrate
- K146 (= K139) active site, Proton acceptor; modified: N6-(pyridoxal phosphate)lysine
- E178 (= E172) binding pyridoxal 5'-phosphate; mutation to K: Loss of transaminase activity and small gain in racemase activity.
- L202 (= L196) mutation to A: Inactivates enzyme.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
3lqsA Complex structure of d-amino acid aminotransferase and 4-amino-4,5- dihydro-thiophenecarboxylic acid (adta) (see paper)
30% identity, 92% coverage: 19:247/249 of query aligns to 22:252/280 of 3lqsA
- active site: Y31 (≠ F28), V33 (≠ T30), K145 (= K139), E177 (= E172), L201 (= L196)
- binding 4-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]thiophene-2-carboxylic acid: V33 (≠ T30), R50 (= R47), E177 (= E172), S180 (= S175), S181 (≠ A176), N182 (= N177), L201 (= L196), G203 (= G198), I204 (≠ V199), T205 (= T200), S240 (≠ N235), T241 (≠ A236), T242 (≠ L237)
3daaA Crystallographic structure of d-amino acid aminotransferase inactivated by pyridoxyl-d-alanine (see paper)
30% identity, 92% coverage: 19:247/249 of query aligns to 22:252/277 of 3daaA
- active site: Y31 (≠ F28), V33 (≠ T30), K145 (= K139), E177 (= E172), L201 (= L196)
- binding n-(5'-phosphopyridoxyl)-d-alanine: Y31 (≠ F28), R50 (= R47), K145 (= K139), E177 (= E172), S180 (= S175), S181 (≠ A176), L201 (= L196), G203 (= G198), I204 (≠ V199), T205 (= T200), S240 (≠ N235), T241 (≠ A236)
2daaA Crystallographic structure of d-amino acid aminotransferase inactivated by d-cycloserine
30% identity, 92% coverage: 19:247/249 of query aligns to 22:252/277 of 2daaA
- active site: Y31 (≠ F28), V33 (≠ T30), K145 (= K139), E177 (= E172), L201 (= L196)
- binding d-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-n,o-cycloserylamide: Y31 (≠ F28), V33 (≠ T30), R50 (= R47), R98 (vs. gap), H100 (vs. gap), K145 (= K139), E177 (= E172), S180 (= S175), S181 (≠ A176), N182 (= N177), L201 (= L196), G203 (= G198), I204 (≠ V199), T205 (= T200), T241 (≠ A236)
1daaA Crystallographic structure of d-amino acid aminotransferase complexed with pyridoxal-5'-phosphate (see paper)
30% identity, 92% coverage: 19:247/249 of query aligns to 22:252/277 of 1daaA
- active site: Y31 (≠ F28), V33 (≠ T30), K145 (= K139), E177 (= E172), L201 (= L196)
- binding pyridoxal-5'-phosphate: R50 (= R47), K145 (= K139), E177 (= E172), S180 (= S175), S181 (≠ A176), L201 (= L196), G203 (= G198), I204 (≠ V199), T205 (= T200), S240 (≠ N235), T241 (≠ A236)
3cswC Crystal structure of a putative branched-chain amino acid aminotransferase (tm0831) from thermotoga maritima at 2.15 a resolution
27% identity, 89% coverage: 26:246/249 of query aligns to 31:240/275 of 3cswC
- active site: Y33 (≠ F28), K135 (= K137), E166 (= E172), L190 (= L196)
- binding pyridoxal-5'-phosphate: R52 (= R47), K135 (= K137), E166 (= E172), F169 (≠ S175), S170 (≠ A176), L190 (= L196), G192 (= G198), I193 (≠ V199), T194 (= T200), T230 (≠ A236)
7z79B Crystal structure of aminotransferase-like protein from variovorax paradoxus
26% identity, 98% coverage: 4:247/249 of query aligns to 17:273/306 of 7z79B
- binding pyridoxal-5'-phosphate: W46 (≠ F28), R65 (= R47), N166 (≠ T141), S202 (= S175), T203 (≠ A176), F222 (≠ L196), G224 (= G198), I225 (≠ V199), T226 (= T200), G261 (≠ N235), T262 (≠ A236)
1a0gB L201a mutant of d-amino acid aminotransferase complexed with pyridoxamine-5'-phosphate (see paper)
29% identity, 92% coverage: 19:247/249 of query aligns to 22:252/282 of 1a0gB
- active site: Y31 (≠ F28), V33 (≠ T30), K145 (= K139), E177 (= E172), A201 (≠ L196)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R50 (= R47), K145 (= K139), E177 (= E172), S180 (= S175), S181 (≠ A176), G203 (= G198), I204 (≠ V199), T205 (= T200), S240 (≠ N235), T241 (≠ A236)
1iyeA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
23% identity, 92% coverage: 19:247/249 of query aligns to 24:265/304 of 1iyeA
- active site: F33 (= F28), G35 (≠ T30), K156 (≠ I138), A157 (≠ K139), E190 (= E172), L214 (= L196)
- binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: R56 (= R47), Y92 (= Y83), Y126 (vs. gap), K156 (≠ I138), Y161 (= Y143), E190 (= E172), G193 (≠ S175), E194 (≠ A176), N195 (= N177), L214 (= L196), G216 (= G198), I217 (≠ V199), T218 (= T200), G253 (≠ N235), T254 (≠ A236), A255 (≠ L237)
1iydA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
23% identity, 92% coverage: 19:247/249 of query aligns to 24:265/304 of 1iydA
- active site: F33 (= F28), G35 (≠ T30), K156 (≠ I138), A157 (≠ K139), E190 (= E172), L214 (= L196)
- binding glutaric acid: Y92 (= Y83), Y126 (vs. gap), A255 (≠ L237)
- binding pyridoxal-5'-phosphate: R56 (= R47), K156 (≠ I138), Y161 (= Y143), E190 (= E172), G193 (≠ S175), E194 (≠ A176), L214 (= L196), G216 (= G198), I217 (≠ V199), T218 (= T200), T254 (≠ A236)
Query Sequence
>WP_012676469.1 NCBI__GCF_000021565.1:WP_012676469.1
MDYIVTVNGKKLIDTRFLRSVMYGEGVFETFRYRGRLPKHIDQHYRRLIKGAELLKIPKI
TKEDYIFYIEETVKKCKESDDLYVKTVLLSEGNIYFPVVPYKSHLLVIVKPYKEPEKKEI
SLAVAPFRVHSSDPMLKIKSTNYSRNIIAKRYALEKGYDDALFLNENGEVTETSSANIFW
IKGRFLYTPSVDCGLLPGVTRSVILKKAKDEGFAVVEGRFYLEDVRKADYIFVANALNGI
IRVSKIEIL
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory