SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012676469.1 NCBI__GCF_000021565.1:WP_012676469.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

8pnyA Crystal structure of d-amino acid aminotransferase from blastococcus saxobsidens complexed with phenylhydrazine and in its apo form (see paper)
25% identity, 98% coverage: 5:247/249 of query aligns to 13:260/278 of 8pnyA

query
sites
8pnyA

V

V

T

T

V

V

N

P

G

A

K

H

K

Q

L

P

I

V

D

V

T

T

R

A

F

F

L

D

R

L

S

G

V

L

M

G

Y

R

G

G

E

D

G

G

V

I

F
|
F

E

E

T
x
S

F

V

R

A

Y

V

-

V

R

A

G

G

R

R

L

T

P

P

K

-

H

H

I

L

D

A

Q

A

H

H

Y

L

R

T

R
|
R

L

L

I

T

K

R

G

S

A

A

E

A

L

L

K

G

I

L

P

P

K

A

I

P

T

G

K

D

E

Q

D

A

Y

W

I

M

F

E

Y

M

I

V

E

A

T

V

V

L

K

A

K

D

C

W

K

P

E

A

S

A

D

L

D

E

L

G

Y

V

V

C

K

R

T

L

V

F

L

L

-

T

-

R

-

G

L

L

S

G

E

D

G

G

N

T

I

P

Y

-

F

-

P

-

V

-

P

P

Y

T

K

A

S

L

H

A

L

L

V

A

I

P

V

V

K

P

P

A

Y

D

K

T

E

L

P

R

E

Q

K

R

K

A

E

E

-

G

I

I

S

S

L

V

A

A

V

T

-

L

-

G

-

L

-

G

-

V

-

P

A

A

P

D

F

F

R

R

V

A

H

G

S

A

S

P

D

W

P

L

M

L

L

G

K

G

I

A

K
|
K

S

T

T

L

N

S

Y
|
Y

S

A

R

V

N

N

I

M

I

A

A

A

K

Q

R

R

Y

H

A

A

L

H

E

D

K

L

G

G

Y

A

D

D

A

V

L

V

F

F

L

T

N

S

E

L

N

E

G

G

E

R

V

L

T

L

E
|
E

T
x
G

S
x
P

S
x
T

A
x
S

N
x
T

I

V

F

V

W

W

I

A

K

A

G

G

R

G

F

T

L

L

Y

H

T

T

P

P

S

P

V

V

D

E

C

T

G

G

L

I

L
|
L

P

P

G

G

V
x
T

T
|
T

R

Q

S

A

V

R

I

L

L

F

K

T

K

A

A

A

K

A

D

A

E

D

G

G

F

W

A

P

V

T

V

S

E

V

G

T

R

P

F

G

Y

T

L

V

E

D

D

D

V

L

R

H

K

A

A

A

D

D

Y

A

I

V

F

W

V

L

A

L

N
x
S

A
x
G

L

V

N

R

G

G

I

A

R

V

V

V

S

H

K

T

I

V

E

D

binding [6-methyl-5-oxidanyl-4-[(2-phenylhydrazinyl)methyl]pyridin-3-yl]methyl dihydrogen phosphate: F36 (= F28), S38 (≠ T30), R55 (= R47), K152 (= K139), Y156 (= Y143), E185 (= E172), G186 (≠ T173), P187 (≠ S174), T188 (≠ S175), S189 (≠ A176), T190 (≠ N177), L209 (= L196), T212 (≠ V199), T213 (= T200), S248 (≠ N235), G249 (≠ A236)

8pnxA Crystal structure of d-amino acid aminotransferase from blastococcus saxobsidens in pmp form
25% identity, 98% coverage: 5:247/249 of query aligns to 13:260/278 of 8pnxA

query
sites
8pnxA

V

V

T

T

V

V

N

P

G

A

K

H

K

Q

L

P

I

V

D

V

T

T

R

A

F

F

L

D

R

L

S

G

V

L

M

G

Y

R

G

G

E

D

G

G

V

I

F

F

E

E

T

S

F

V

R

A

Y

V

-

V

R

A

G

G

R

R

L

T

P

P

K

-

H

H

I

L

D

A

Q

A

H

H

Y

L

R

T

R
|
R

L

L

I

T

K

R

G

S

A

A

E

A

L

L

K

G

I

L

P

P

K

A

I

P

T

G

K

D

E

Q

D

A

Y

W

I

M

F

E

Y

M

I

V

E

A

T

V

V

L

K

A

K

D

C

W

K

P

E

A

S

A

D

L

D

E

L

G

Y

V

V

C

K

R

T

L

V

F

L

L

-

T

-

R

-

G

L

L

S

G

E

D

G

G

N

T

I

P

Y

-

F

-

P

-

V

-

P

P

Y

T

K

A

S

L

H

A

L

L

V

A

I

P

V

V

K

P

P

A

Y

D

K

T

E

L

P

R

E

Q

K

R

K

A

E

E

-

G

I

I

S

S

L

V

A

A

V

T

-

L

-

G

-

L

-

G

-

V

-

P

A

A

P

D

F

F

R

R

V

A

H

G

S

A

S

P

D

W

P

L

M

L

L

G

K

G

I

A

K
|
K

S

T

T

L

N

S

Y
|
Y

S

A

R

V

N

N

I

M

I

A

A

A

K

Q

R

R

Y

H

A

A

L

H

E

D

K

L

G

G

Y

A

D

D

A

V

L

V

F

F

L

T

N

S

E

L

N

E

G

G

E

R

V

L

T

L

E
|
E

T

G

S

P

S
x
T

A
x
S

N
x
T

I

V

F

V

W

W

I

A

K

A

G

G

R

G

F

T

L

L

Y

H

T

T

P

P

S

P

V

V

D

E

C

T

G

G

L

I

L
|
L

P

P

G

G

V
x
T

T
|
T

R

Q

S

A

V

R

I

L

L

F

K

T

K

A

A

A

K

A

D

A

E

D

G

G

F

W

A

P

V

T

V

S

E

V

G

T

R

P

F

G

Y

T

L

V

E

D

D

D

V

L

R

H

K

A

A

A

D

D

Y

A

I

V

F

W

V

L

A

L

N
x
S

A
x
G

L

V

N

R

G

G

I

A

R

V

V

V

S

H

K

T

I

V

E

D

binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R55 (= R47), K152 (= K139), Y156 (= Y143), E185 (= E172), T188 (≠ S175), S189 (≠ A176), T190 (≠ N177), L209 (= L196), T212 (≠ V199), T213 (= T200), S248 (≠ N235), G249 (≠ A236)

8pnwA Crystal structure of d-amino acid aminotransferase from blastococcus saxobsidens in holo form with plp (see paper)
25% identity, 98% coverage: 5:247/249 of query aligns to 13:260/278 of 8pnwA

query
sites
8pnwA

V

V

T

T

V

V

N

P

G

A

K

H

K

Q

L

P

I

V

D

V

T

T

R

A

F

F

L

D

R

L

S

G

V

L

M

G

Y

R

G

G

E

D

G

G

V

I

F

F

E

E

T

S

F

V

R

A

Y

V

-

V

R

A

G

G

R

R

L

T

P

P

K

-

H

H

I

L

D

A

Q

A

H

H

Y

L

R

T

R
|
R

L

L

I

T

K

R

G

S

A

A

E

A

L

L

K

G

I

L

P

P

K

A

I

P

T

G

K

D

E

Q

D

A

Y

W

I

M

F

E

Y

M

I

V

E

A

T

V

V

L

K

A

K

D

C

W

K

P

E

A

S

A

D

L

D

E

L

G

Y

V

V

C

K

R

T

L

V

F

L

L

-

T

-

R

-

G

L

L

S

G

E

D

G

G

N

T

I

P

Y

-

F

-

P

-

V

-

P

P

Y

T

K

A

S

L

H

A

L

L

V

A

I

P

V

V

K

P

P

A

Y

D

K

T

E

L

P

R

E

Q

K

R

K

A

E

E

-

G

I

I

S

S

L

V

A

A

V

T

-

L

-

G

-

L

-

G

-

V

-

P

A

A

P

D

F

F

R

R

V

A

H

G

S

A

S

P

D

W

P

L

M

L

L

G

K

G

I

A

K
|
K

S

T

T

L

N

S

Y
|
Y

S

A

R

V

N

N

I

M

I

A

A

A

K

Q

R

R

Y

H

A

A

L

H

E

D

K

L

G

G

Y

A

D

D

A

V

L

V

F

F

L

T

N

S

E

L

N

E

G

G

E

R

V

L

T

L

E
|
E

T

G

S
x
P

S
x
T

A
x
S

N

T

I

V

F

V

W

W

I

A

K

A

G

G

R

G

F

T

L

L

Y

H

T

T

P

P

S

P

V

V

D

E

C

T

G

G

L

I

L
|
L

P

P

G

G

V
x
T

T
|
T

R

Q

S

A

V

R

I

L

L

F

K

T

K

A

A

A

K

A

D

A

E

D

G

G

F

W

A

P

V

T

V

S

E

V

G

T

R

P

F

G

Y

T

L

V

E

D

D

D

V

L

R

H

K

A

A

A

D

D

Y

A

I

V

F

W

V

L

A

L

N

S

A
x
G

L

V

N

R

G

G

I

A

R

V

V

V

S

H

K

T

I

V

E

D

binding pyridoxal-5'-phosphate: R55 (= R47), K152 (= K139), Y156 (= Y143), E185 (= E172), P187 (≠ S174), T188 (≠ S175), S189 (≠ A176), L209 (= L196), T212 (≠ V199), T213 (= T200), G249 (≠ A236)

5mr0D Thermophilic archaeal branched-chain amino acid transaminases from geoglobus acetivorans and archaeoglobus fulgidus: biochemical and structural characterisation (see paper)
35% identity, 88% coverage: 17:234/249 of query aligns to 21:244/290 of 5mr0D

query
sites
5mr0D

F

F

L

D

R

H

S

G

V

F

M

L

Y

Y

G

G

E

D

G

G

V

V

F
|
F

E

E

T
x
G

F

I

R

R

-

A

Y

Y

R

N

G

G

R

R

-

V

-

F

-

R

L

L

P

K

K

E

H

H

I

I

D

D

Q
x
R

H

L

Y

Y

R

D

R

S

L

-

I

-

K

A

G

K

A

A

E

I

L

D

L

L

K

E

I

I

P

P

K

-

I

I

T

T

K

K

E

E

D

E

Y

F

I

M

F

E

Y

I

I

I

E

L

E

E

T

T

V

L

K

R

K

K

C

N

K

N

E

L

S

R

D

D

D

A

L

Y

Y

I

V

R

K

P

T

I

V

V

L

T

L

R

S

G

E

I

G

G

N

D

I

L

Y
x
G

F
x
L

-

D

P

P

V

R

V

K

P

C

Y

Q

K

N

S

P

H

S

L

I

V

V

I

I

V

T

K

K

P

P

-

W

-

G

-

K

-

L

Y

Y

K

G

E

D

P

L

E

Y

K

E

K

K

E

G

I

L

S

T

L

A

A

I

V

T

A

V

P

A

F

V

R

R

V

R

H

N

S

S

S

F

D

D

P

A

M

L

L

P

-

P

K

N

I

I

K
|
K

S

S

T

L

N

N

Y
|
Y

S

L

R

N

N

N

I

I

I

L

A

A

K

K

R

I

Y

E

A

A

L

N

E

A

K

K

G

G

Y

G

D

D

D

E

A

A

L

I

F

F

L

L

N

D

E

R

N

N

G

G

E

Y

V

V

T

S

E
|
E

T

G

S

S

S
x
G

A
x
D

N

N

I

I

F

F

W

V

I

V

K

K

G

N

R

G

F

A

L

I

Y

T

T

T

P

P

S

P

V

T

D

-

C

I

G

N

L

N

L
|
L

P

R

G

G

V
x
I

T
|
T

R

R

S

E

V

A

I

V

L

I

K

E

K

I

A

I

K

N

D

R

E

L

G

G

F

I

A

P

V

F

V

K

E

E

G

T

R

N

F

I

Y

G

L

L

E

Y

D

D

V

L

R

Y

K

T

A

A

D

D

Y

E

I

V

F

F

V

V

A

T

active site: F32 (= F28), G34 (≠ T30), K150 (= K139), E183 (= E172), L206 (= L196)
binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: R51 (≠ Q43), G100 (≠ Y95), L101 (≠ F96), K150 (= K139), Y154 (= Y143), E183 (= E172), G186 (≠ S175), D187 (≠ A176), L206 (= L196), I209 (≠ V199), T210 (= T200)

Sites not aligning to the query:

binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: 245, 246

5e25A Crystal structure of branched-chain aminotransferase from thermophilic archaea geoglobus acetivorans complexed with alpha-ketoglutarate (see paper)
33% identity, 93% coverage: 17:247/249 of query aligns to 22:258/290 of 5e25A

query
sites
5e25A

F

F

L

D

R

H

S

G

V

F

M

L

Y

Y

G

G

E

D

G

G

V

V

F
|
F

E

E

T
x
G

F

I

R

R

-

A

Y

Y

R

N

G

G

R

K

L

V

P

F

K

K

-

L

-

Y

-

E

H

H

I

I

D

D

Q
x
R

H

L

Y

Y

-

D

-

C

R

A

R

R

L

V

I

I

K

D

G

-

A

-

E

-

L

-

L

L

K

K

I

I

P

P

K

-

I

L

T

S

K

K

E

E

D

E

Y

F

I

A

F

E

Y

A

I

I

E

L

E

E

T

T

V

L

K

R

C

N

K

N

E

L

S

R

D

D

D

A

L
x
Y

Y

I

V

R

K

P

T

I

V

V

L

T

L

R

S

G

E

A

G

G

N

D

I

L

Y

G

F

L

-

D

P

P

V

R

V

K

P

C

Y

P

K

S

S

P

H

N

L

V

L

I

V

I

I

I

V

T

K

K

P

P

Y

W

K

G

-

K

-

L

-

Y

-

G

-

D

-

L

-

Y

E

E

P

K

E

G

K

L

K

K

E

A

I

I

S

T

L

V

A

A

V

I

A

R

P

R

F

N

R

A

V

I

H

D

S

S

S

L

D

P

P

P

M

-

L

-

K

N

I

I

K
|
K

S

S

T

L

N

N

Y
|
Y

S

L

R

N

N

N

I

I

I

L

A

A

K

K

R

I

Y

E

A

A

L

N

E

A

K

K

G

G

Y

G

D

D

D

E

A

A

L

I

F

F

L

L

N

D

E

H

N

N

G

G

E

Y

V

I

T

S

E
|
E

T

G

S

S

S
x
G

A
x
D

N

N

I

I

F

F

W

I

I

V

K

K

G

N

R

G

F

T

L

I

Y

T

T

T

P

P

S

P

V

T

D

-

C

L

G

N

L

N

L
|
L

P

K

G
|
G

V
x
I

T
|
T

R

R

S

Q

V

V

I

V

L

I

K

E

K

L

A

I

K

N

D

E

E

L

G

E

F

I

A

P

V

F

V

R

E

E

G

A

R

N

F

I

Y

G

L

L

E

F

D

D

V

L

R

Y

K

S

A

A

D

D

Y

E

I

I

F

F

V

V

A

T

N
x
G

A
x
T

L
x
A

N

A

G

E

I

I

I

A

R

P

V

V

S

T

K

Y

I

I

E

D

active site: F33 (= F28), G35 (≠ T30), K151 (= K139), E184 (= E172), L207 (= L196)
binding 2-oxoglutaric acid: Y88 (≠ L82), K151 (= K139), T247 (≠ A236), A248 (≠ L237)
binding pyridoxal-5'-phosphate: R52 (≠ Q43), K151 (= K139), Y155 (= Y143), E184 (= E172), G187 (≠ S175), D188 (≠ A176), L207 (= L196), G209 (= G198), I210 (≠ V199), T211 (= T200), G246 (≠ N235), T247 (≠ A236)

6thqB Crystal structure of branched-chain aminotransferase from thermophilic archaea thermoproteus uzoniensis with norvaline
32% identity, 86% coverage: 20:232/249 of query aligns to 29:250/301 of 6thqB

query
sites
6thqB

S

S

V

L

M

N

Y

Y

G

G

E

F

G

G

V

V

F
|
F

E

E

T

G

F

I

R

R

-

A

-

Y

-

W

-

N

-

G

-

E

-

N

-

L

Y

Y

R

V

G

F

R

R

L

L

P

R

K

D

H

H

I

M

D

E

Q

-

H

-

Y

-

R

-

R
|
R

L

L

I

L

K

R

G

S

A

A

E

K

L

I

-

I

-

G

L

L

K

D

I

V

P

P

K

-

I

Y

T

T

K

A

E

E

D

E

Y

L

I

S

F

K

Y

A

I

V

E

V

E

E

T

T

V

V

K

R

K

A

C

N

K

G

E

F

S

K

D

E

D

D

L

L

Y
|
Y

V

I

K

R

T

P

V

V

L

A

-

Y

L

I

S

S

E

K

G

P

N

Q

I

I

Y

S

F

L

P

D

V

V

V

R

P

G

Y

L

K

Q

S

A

H

S

L

V

L

A

V

I

I

A

V

A

K

I

P

P

Y

F

K

G

E

K

P

Y

E

L

K

K

K

V

E

E

-

G

I

V

S

R

L

A

A

A

V

V

A

V

P

S

F

W

R

R

-

R

V

V

H

H

S

T

S

S

D

M

P

M

M

P

L

V

K

M

I

A

K
|
K

S

A

T

T

N

G

-

I

Y
|
Y

S

L

R

N

N

S

I

I

I

M

A

A

K

A

R

V

Y

E

A

A

L

R

E

A

K

R

G

G

Y

Y

D

D

D

E

A

A

L

I

F

M

L

L

N

N

E

A

N

E

G

G

E

K

V

V

T

V

E
|
E

T

G

S

S

S
x
G

A
x
E

N
|
N

I

I

F

F

W

I

I

V

K

R

G

R

R

G

F

V

L

L

Y

M

T

T

P

P

S

P

V

L

D

E

C

D

G

G

L

I

L
|
L

P

E

G
|
G

V
x
I

T
|
T

R

R

S

E

V

T

I

V

L

I

K

S

K

I

A

A

K

G

D

D

E

L

G

G

F

I

A

P

V

L

E

E

G

K

R

S

F

I

Y

T

L

R

E

E

D

E

V

L

R

Y

K

A

A

A

D

D

Y

E

I

A

F

F

active site: F37 (= F28), K156 (= K139), E190 (= E172), L214 (= L196)
binding pyridoxal-5'-phosphate: R60 (= R47), K156 (= K139), Y161 (= Y143), E190 (= E172), N195 (= N177), L214 (= L196), G216 (= G198), I217 (≠ V199), T218 (= T200)
binding 2-[o-phosphonopyridoxyl]-amino-pentanoic acid: R60 (= R47), Y97 (= Y83), K156 (= K139), Y161 (= Y143), E190 (= E172), G193 (≠ S175), E194 (≠ A176), N195 (= N177), G216 (= G198), I217 (≠ V199), T218 (= T200)

Sites not aligning to the query:

1i2lA Deoxychorismate lyase from escherichia coli with inhibitor
26% identity, 92% coverage: 19:246/249 of query aligns to 17:247/269 of 1i2lA

query
sites
1i2lA

R

R

S

A

V

T

M

Q

Y

F

G

G

E

D

G

G

V

C

F
|
F

E

T

T
|
T

F

A

R

R

Y

V

R

I

G

D

R

G

L

K

P

V

K

S

H

L

I

L

D

S

Q

A

H

H

Y

I

R

Q

R
|
R

L

L

I

Q

K

D

G

A

A

C

E

Q

L

R

L

L

K

M

I

I

P

S

K

C

I

-

T

-

K

-

E

-

D

D

Y

F

I

W

F

P

Y

Q

I

L

E

E

E

Q

T

E

V

M

K

K

K

T

C

L

K

A

E

A

S

E

D

Q

L

N

Y

G

V

V

K

L

T

K

V

V

L

V

L

I

S

S

E

R

G

G

N

S

-

G

-

R

-

G

-

Y

I

S

Y

T

F

L

P

N

V

S

V

G

P

P

Y

A

K

T

S

R

H

I

L

L

L

S

V

V

I

T

V

A

K

Y

P

P

-

A

-

H

Y

Y

K

D

E

R

P

L

E

R

K

N

K

E

E

G

I

I

S

T

L

L

A

A

V

L

A

S

P

P

F

V

R

R

V

L

H

G

S

R

S

N

D

P

P

H

M

L

L

A

K

G

I
|
I

K
|
K

S

H

T

L

N

N

Y

R

S

L

R

E

N

Q

I

V

I

L

A

I

K

R

R

S

Y

H

A

L

L

E

E

Q

K

T

G

N

Y

A

D

D

D

E

A

A

L

L

F

V

L

L

N

D

E

S

N

E

G

G

E

W

V

V

T

T

E
|
E

T

C

S
x
C

S
x
A

A
|
A

N
|
N

I

L

F

F

W

W

I

R

K

K

G

G

R

N

F

V

L

V

Y

Y

T

T

P

P

S

R

V

L

D

D

C

Q

G

A

L

G

L
x
V

P

N

G
|
G

V
x
I

T
x
M

R

R

S

Q

V

F

I

C

L

I

K

R

K

L

A

L

K

A

D

Q

E

S

G

S

F

Y

A

Q

V

L

V

V

E

E

G

V

R

Q

F

A

Y

S

L

L

E

E

D

E

V

S

R

L

K

Q

A

A

D

D

Y

E

I

M

F

V

V

I

A

C

N
|
N

A
|
A

L

L

N

M

G

P

I

V

I

M

R

P

V

V

S

C

K

A

I

C

active site: F26 (= F28), T28 (= T30), I139 (= I138), K140 (= K139), E173 (= E172), V197 (≠ L196)
binding d-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-n,o-cycloserylamide: R45 (= R47), K140 (= K139), E173 (= E172), C175 (≠ S174), A176 (≠ S175), A177 (= A176), N178 (= N177), V197 (≠ L196), G199 (= G198), I200 (≠ V199), M201 (≠ T200), N236 (= N235), A237 (= A236)

1i2kA Aminodeoxychorismate lyase from escherichia coli
26% identity, 92% coverage: 19:246/249 of query aligns to 17:247/269 of 1i2kA

query
sites
1i2kA

R

R

S

A

V

T

M

Q

Y

F

G

G

E

D

G

G

V

C

F
|
F

E

T

T
|
T

F

A

R

R

Y

V

R

I

G

D

R

G

L

K

P

V

K

S

H

L

I

L

D

S

Q

A

H

H

Y

I

R

Q

R
|
R

L

L

I

Q

K

D

G

A

A

C

E

Q

L

R

L

L

K

M

I

I

P

S

K

C

I

-

T

-

K

-

E

-

D

D

Y

F

I

W

F

P

Y

Q

I

L

E

E

E

Q

T

E

V

M

K

K

K

T

C

L

K

A

E

A

S

E

D

Q

L

N

Y

G

V

V

K

L

T

K

V

V

L

V

L

I

S

S

E

R

G

G

N

S

-

G

-

R

-

G

-

Y

I

S

Y

T

F

L

P

N

V

S

V

G

P

P

Y

A

K

T

S

R

H

I

L

L

L

S

V

V

I

T

V

A

K

Y

P

P

-

A

-

H

Y

Y

K

D

E

R

P

L

E

R

K

N

K

E

E

G

I

I

S

T

L

L

A

A

V

L

A

S

P

P

F

V

R

R

V

L

H

G

S

R

S

N

D

P

P

H

M

L

L

A

K

G

I
|
I

K
|
K

S

H

T

L

N

N

Y

R

S

L

R

E

N

Q

I

V

I

L

A

I

K

R

R

S

Y

H

A

L

L

E

E

Q

K

T

G

N

Y

A

D

D

D

E

A

A

L

L

F

V

L

L

N

D

E

S

N

E

G

G

E

W

V

V

T

T

E
|
E

T

C

S

C

S
x
A

A
|
A

N

N

I

L

F

F

W

W

I

R

K

K

G

G

R

N

F

V

L

V

Y

Y

T

T

P

P

S

R

V

L

D

D

C

Q

G

A

L

G

L
x
V

P

N

G
|
G

V
x
I

T
x
M

R

R

S

Q

V

F

I

C

L

I

K

R

K

L

A

L

K

A

D

Q

E

S

G

S

F

Y

A

Q

V

L

V

V

E

E

G

V

R

Q

F

A

Y

S

L

L

E

E

D

E

V

S

R

L

K

Q

A

A

D

D

Y

E

I

M

F

V

V

I

A

C

N
|
N

A
|
A

L

L

N

M

G

P

I

V

I

M

R

P

V

V

S

C

K

A

I

C

active site: F26 (= F28), T28 (= T30), I139 (= I138), K140 (= K139), E173 (= E172), V197 (≠ L196)
binding pyridoxal-5'-phosphate: R45 (= R47), K140 (= K139), E173 (= E172), A176 (≠ S175), A177 (= A176), V197 (≠ L196), G199 (= G198), I200 (≠ V199), M201 (≠ T200), N236 (= N235), A237 (= A236)

7p3tB Transaminase of gamma-proteobacterium (see paper)
28% identity, 92% coverage: 19:246/249 of query aligns to 25:258/299 of 7p3tB

query
sites
7p3tB

R

Q

S

G

V

F

M

L

Y

L

G

G

E

D

G

G

V

V

F

F

E

D

T

V

F

V

R

S

-

A

Y

W

R

K

G

G

R

N

L

I

P

F

K

K

H

-

I

L

D

D

Q

A

H

H

Y

L

R

D

R
|
R

L

F

I

F

K

D

G

S

A

I

E

Q

L

A

K

R

I

L

P

N

K

H

-

D

I

M

T

S

K

R

E

D

D

A

Y

W

I

K

F

E

Y

A

I

I

E

I

E

E

T

T

V

T

K

R

C

-

K

N

E

G

S

L

D

D

L

A

Y

S

V

I

K

R

T

F

V

I

L

V

L

T

-

R

-

G

-

E

S

P

E

K

G

G

N

V

I

V

Y

A

F

D

P

P

V

R

V

-

P

D

Y

F

K

K

S

P

H

T

L

C

L

I

V

V

I

W

V

V

K

A

P

P

Y

Y

-

I

-

F

-

L

-

A

-

D

K

E

E

E

P

K

E

R

K

R

K

N

E

G

I

I

S

R

L

L

A

M

V

I

A

S

P

A

-

T

-

R

-

G

F

F

R

P

V

A

H

D

S

T

S

L

D

D

P

P

M

R

L

Y

K
|
K

I

C

K

L

S

D

T
x
R

N

L

Y

H

S

S

R

Q

N

L

I

I

I

-

A

-

K

R

R

L

Y

E

A

A

L

L

E

E

K

A

G

G

Y

Y

D

D

A

A

L

L

F

W

L

L

N

D

E

H

N

S

G

G

E

H

V

V

T

S

E
|
E

T
x
S

S
x
A

A
x
S

N

N

I

L

F

F

W

I

I

V

K

K

G

N

R

G

F

V

L

L

Y

Y

T

T

P

P

S

S

V

-

D

-

C

A

G

G

L

I

L

L

P

R

G
|
G

V
x
I

T
|
T

R

R

S

D

V

T

I

I

L

L

K

E

K

L

A

A

K

T

D

E

E

L

G

D

F

I

A

P

V

W

V

K

E

E

G

R

R

Q

F

L

Y

S

L

A

E

F

D

D

V

V

R

Y

K

I

A

A

D

D

Y

E

I

V

F

F

V

T

A

C

N

S

A
x
T

L

A

N

G

G

G

I

A

I

L

R

P

V

V

S

R

K

E

I

V

binding pyridoxal-5'-phosphate: R53 (= R47), K153 (= K137), R157 (≠ T141), E186 (= E172), S187 (≠ T173), A188 (≠ S174), A189 (≠ S175), S190 (≠ A176), G210 (= G198), I211 (≠ V199), T212 (= T200), T248 (≠ A236)

4whxA X-ray crystal structure of an amino acid aminotransferase from burkholderia pseudomallei bound to the co-factor pyridoxal phosphate
26% identity, 89% coverage: 11:232/249 of query aligns to 14:253/306 of 4whxA

query
sites
4whxA

K

K

L

L

I

I

D

E

T

W

R

R

-

D

-

A

-

K

-

I

-

H

-

V

F

L

L

T

R

H

S

T

V

L

M

H

Y

Y

G

G

E

M

G

G

V

V

F
|
F

E

E

T

G

F

V

R

R

-

A

Y

Y

R

K

-

T

-

A

-

D

-

G

G

G

R

T

L

A

P

I

K

F

H

R

I

L

D

K

Q

E

H

H

Y

T

R

K

R

R

L

L

I

L

K

N

G

S

A

A

E

K

L

I

L

F

K

Q

I

M

P

D

K

V

I

P

T

F

K

D

E

Q

D

E

Y

T

I

L

F

E

Y

A

I

A

E

Q

E

R

T

D

V

V

K

V

K

R

C

E

K

N

E

K

S

L

D

E

D

S

L

C

Y
|
Y

V

L

K

R

T

P

V

I

L

I

-

W

L

I

S

G

E

S

G

E

N

K

I

L

Y

G

F

V

P

S

V

A

V

K

P

G

Y

N

K

T

S

I

H

H

L

V

L

A

V

I

I

A

V

A

K

W

P

P

Y

W

-

G

-

A

-

Y

-

L

-

G

K

E

E

E

P

G

E

L

K

A

K

K

E

G

I

I

S

R

L

V

A

K

V

T

A

S

P

S

F

F

-

T

R

R

V

H

H

H

S

V

S

N

D

V

P

S

M

M

L

V

K

R

I

A

K
|
K

S

A

T

S

N

G

-

W

Y

Y

S

V

R

N

N

S

I

I

I

L

A

A

K

N

R

Q

Y

E

A

A

L

T

E

A

K

D

G

G

Y

Y

D

D

D

E

A

A

L

L

F

L

L

L

N

D

E

V

N

D

G

G

E

Y

V

V

T

S

E
|
E

T

G

S

S

S

G

A

E

N

N

I

F

F

F

W

L

I

V

K

N

G

R

R

G

F

K

L

L

Y

Y

T

T

P

P

S

D

V

L

D

-

C

A

G

S

L

C

L
|
L

P

D

G

G

V

I

T

T

R

R

S

D

V

T

I

V

L

I

K

T

K

L

A

A

K

K

D

E

E

A

G

G

F

I

A

E

V

V

V

I

E

E

G

K

R

R

F

I

Y

T

L

R

E

D

D

E

V

V

R

Y

K

T

A

A

D

D

Y

E

I

A

F

F

active site: F37 (= F28), K160 (= K139), E194 (= E172), L217 (= L196)
binding alanine: Y97 (= Y83), K160 (= K139)

Sites not aligning to the query:

binding alanine: 258

P19938 D-alanine aminotransferase; D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase; EC 2.6.1.21 from Bacillus sp. (strain YM-1) (see 5 papers)
30% identity, 92% coverage: 19:247/249 of query aligns to 23:253/283 of P19938

query
sites
P19938

R

R

S

G

V

Y

M

Q

Y

F

G

G

E

D

G

G

V

V

F
x
Y

E

E

T

V

F

V

R

K

-

V

Y

Y

R

N

G

G

R

E

L

M

P

F

K

T

H

-

I

V

D

N

Q

E

H

H

Y

I

R

D

R
|
R

L

L

I

Y

K

A

G

S

A

A

E

E

L

K

L

I

K

R

I

I

P

T

-

I

K

P

I

Y

T

T

K

K

E

D

D

K

Y

F

I

H

F

Q

Y

L

I

L

E

H

E

E

T

L

V

V

K

E

K

K

C

N

K

E

E

-

S

-

D

-

L

-

Y

-

V

-

K

-

T

-

V

-

L

-

L

L

S

N

E

T

G

G

N

H

I

I

Y

Y

F

F

P

Q

V

V

V

T

-

R

-

G

-

T

-

S

-

P

-
x
R

-

A

-
x
H

-

Q

-

F

P

P

Y

E

K

N

S

T

H

V

L

K

L

P

V

V

I

I

V

I

-

G

-

Y

-

T

-

K

-

E

-

N

-

P

K

R

P

P

Y

L

K

E

E

N

P

L

E

E

K

K

K

G

E

V

I

K

S

A

L

T

A

F

V

V

A

E

P

D

F

I

R

R

V

W

H

L

S

R

S

C

D

D

P

-

M

-

L

-

K

-

I

I

K
|
K

S

S

T

L

N

N

Y

L

S

L

R

G

N

A

I

V

I

L

A

A

K

K

R

Q

Y

E

A

A

L

H

E

E

K

K

G

G

Y

C

D

Y

D

E

A

A

L

I

F

-

L

L

N

H

E

R

N

N

G

N

E

T

V

V

T

T

E
|
E

T

G

S

S

A

S

N

N

I

V

F

F

W

G

I

I

K

K

G

D

R

G

F

I

L

L

Y

Y

T

T

P

H

S

P

V

A

D

N

C

N

G

M

L

I

L
|
L

P

K

G

G

V

I

T

T

R

R

S

D

V

V

I

V

L

I

K

A

K

C

A

A

K

N

D

E

E

I

G

N

F

M

A

P

V

V

V

K

E

E

G

I

R

P

F

F

Y

T

L

T

E

H

D

E

V

A

R

L

K

K

A

M

D

D

Y

E

I

L

F

F

V

V

A

T

N

S

A

T

L

T

N

S

G

E

I

I

I

T

R

P

V

V

S

I

K

E

I

I

E

D

Y32 (≠ F28) binding substrate
R51 (= R47) binding pyridoxal 5'-phosphate
R99 (vs. gap) binding substrate
H101 (vs. gap) binding substrate
K146 (= K139) active site, Proton acceptor; modified: N6-(pyridoxal phosphate)lysine
E178 (= E172) binding pyridoxal 5'-phosphate; mutation to K: Loss of transaminase activity and small gain in racemase activity.
L202 (= L196) mutation to A: Inactivates enzyme.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

3lqsA Complex structure of d-amino acid aminotransferase and 4-amino-4,5- dihydro-thiophenecarboxylic acid (adta) (see paper)
30% identity, 92% coverage: 19:247/249 of query aligns to 22:252/280 of 3lqsA

query
sites
3lqsA

R

R

S

G

V

Y

M

Q

Y

F

G

G

E

D

G

G

V

V

F
x
Y

E

E

T
x
V

F

V

R

K

-

V

Y

Y

R

N

G

G

R

E

L

M

P

F

K

T

H

-

I

V

D

N

Q

E

H

H

Y

I

R

D

R
|
R

L

L

I

Y

K

A

G

S

A

A

E

E

L

K

L

I

K

R

I

I

P

T

-

I

K

P

I

Y

T

T

K

K

E

D

D

K

Y

F

I

H

F

Q

Y

L

I

L

E

H

E

E

T

L

V

V

K

E

K

K

C

N

K

E

E

-

S

-

D

-

L

-

Y

-

V

-

K

-

T

-

V

-

L

-

L

L

S

N

E

T

G

G

N

H

I

I

Y

Y

F

F

P

Q

V

V

V

T

-

R

-

G

-

T

-

S

-

P

-

R

-

A

-

H

-

Q

-

F

P

P

Y

E

K

N

S

T

H

V

L

K

L

P

V

V

I

I

V

I

-

G

-

Y

-

T

-

K

-

E

-

N

-

P

K

R

P

P

Y

L

K

E

E

N

P

L

E

E

K

K

K

G

E

V

I

K

S

A

L

T

A

F

V

V

A

E

P

D

F

I

R

R

V

W

H

L

S

R

S

C

D

D

P

-

M

-

L

-

K

-

I

I

K
|
K

S

S

T

L

N

N

Y

L

S

L

R

G

N

A

I

V

I

L

A

A

K

K

R

Q

Y

E

A

A

L

H

E

E

K

K

G

G

Y

C

D

Y

D

E

A

A

L

I

F

-

L

L

N

H

E

R

N

N

G

N

E

T

V

V

T

T

E
|
E

T

G

S

S

S
|
S

A
x
S

N
|
N

I

V

F

F

W

G

I

I

K

K

G

D

R

G

F

I

L

L

Y

Y

T

T

P

H

S

P

V

A

D

N

C

N

G

M

L

I

L
|
L

P

K

G
|
G

V
x
I

T
|
T

R

R

S

D

V

V

I

V

L

I

K

A

K

C

A

A

K

N

D

E

E

I

G

N

F

M

A

P

V

V

V

K

E

E

G

I

R

P

F

F

Y

T

L

T

E

H

D

E

V

A

R

L

K

K

A

M

D

D

Y

E

I

L

F

F

V

V

A

T

N
x
S

A
x
T

L
x
T

N

S

G

E

I

I

I

T

R

P

V

V

S

I

K

E

I

I

E

D

active site: Y31 (≠ F28), V33 (≠ T30), K145 (= K139), E177 (= E172), L201 (= L196)
binding 4-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]thiophene-2-carboxylic acid: V33 (≠ T30), R50 (= R47), E177 (= E172), S180 (= S175), S181 (≠ A176), N182 (= N177), L201 (= L196), G203 (= G198), I204 (≠ V199), T205 (= T200), S240 (≠ N235), T241 (≠ A236), T242 (≠ L237)

3daaA Crystallographic structure of d-amino acid aminotransferase inactivated by pyridoxyl-d-alanine (see paper)
30% identity, 92% coverage: 19:247/249 of query aligns to 22:252/277 of 3daaA

query
sites
3daaA

R

R

S

G

V

Y

M

Q

Y

F

G

G

E

D

G

G

V

V

F
x
Y

E

E

T
x
V

F

V

R

K

-

V

Y

Y

R

N

G

G

R

E

L

M

P

F

K

T

H

-

I

V

D

N

Q

E

H

H

Y

I

R

D

R
|
R

L

L

I

Y

K

A

G

S

A

A

E

E

L

K

L

I

K

R

I

I

P

T

-

I

K

P

I

Y

T

T

K

K

E

D

D

K

Y

F

I

H

F

Q

Y

L

I

L

E

H

E

E

T

L

V

V

K

E

K

K

C

N

K

E

E

-

S

-

D

-

L

-

Y

-

V

-

K

-

T

-

V

-

L

-

L

L

S

N

E

T

G

G

N

H

I

I

Y

Y

F

F

P

Q

V

V

V

T

-

R

-

G

-

T

-

S

-

P

-

R

-

A

-

H

-

Q

-

F

P

P

Y

E

K

N

S

T

H

V

L

K

L

P

V

V

I

I

V

I

-

G

-

Y

-

T

-

K

-

E

-

N

-

P

K

R

P

P

Y

L

K

E

E

N

P

L

E

E

K

K

K

G

E

V

I

K

S

A

L

T

A

F

V

V

A

E

P

D

F

I

R

R

V

W

H

L

S

R

S

C

D

D

P

-

M

-

L

-

K

-

I

I

K
|
K

S

S

T

L

N

N

Y

L

S

L

R

G

N

A

I

V

I

L

A

A

K

K

R

Q

Y

E

A

A

L

H

E

E

K

K

G

G

Y

C

D

Y

D

E

A

A

L

I

F

-

L

L

N

H

E

R

N

N

G

N

E

T

V

V

T

T

E
|
E

T

G

S

S

S
|
S

A
x
S

N

N

I

V

F

F

W

G

I

I

K

K

G

D

R

G

F

I

L

L

Y

Y

T

T

P

H

S

P

V

A

D

N

C

N

G

M

L

I

L
|
L

P

K

G
|
G

V
x
I

T
|
T

R

R

S

D

V

V

I

V

L

I

K

A

K

C

A

A

K

N

D

E

E

I

G

N

F

M

A

P

V

V

V

K

E

E

G

I

R

P

F

F

Y

T

L

T

E

H

D

E

V

A

R

L

K

K

A

M

D

D

Y

E

I

L

F

F

V

V

A

T

N
x
S

A
x
T

L

T

N

S

G

E

I

I

I

T

R

P

V

V

S

I

K

E

I

I

E

D

active site: Y31 (≠ F28), V33 (≠ T30), K145 (= K139), E177 (= E172), L201 (= L196)
binding n-(5'-phosphopyridoxyl)-d-alanine: Y31 (≠ F28), R50 (= R47), K145 (= K139), E177 (= E172), S180 (= S175), S181 (≠ A176), L201 (= L196), G203 (= G198), I204 (≠ V199), T205 (= T200), S240 (≠ N235), T241 (≠ A236)

2daaA Crystallographic structure of d-amino acid aminotransferase inactivated by d-cycloserine
30% identity, 92% coverage: 19:247/249 of query aligns to 22:252/277 of 2daaA

query
sites
2daaA

R

R

S

G

V

Y

M

Q

Y

F

G

G

E

D

G

G

V

V

F
x
Y

E

E

T
x
V

F

V

R

K

-

V

Y

Y

R

N

G

G

R

E

L

M

P

F

K

T

H

-

I

V

D

N

Q

E

H

H

Y

I

R

D

R
|
R

L

L

I

Y

K

A

G

S

A

A

E

E

L

K

L

I

K

R

I

I

P

T

-

I

K

P

I

Y

T

T

K

K

E

D

D

K

Y

F

I

H

F

Q

Y

L

I

L

E

H

E

E

T

L

V

V

K

E

K

K

C

N

K

E

E

-

S

-

D

-

L

-

Y

-

V

-

K

-

T

-

V

-

L

-

L

L

S

N

E

T

G

G

N

H

I

I

Y

Y

F

F

P

Q

V

V

V

T

-

R

-

G

-

T

-

S

-

P

-
x
R

-

A

-
x
H

-

Q

-

F

P

P

Y

E

K

N

S

T

H

V

L

K

L

P

V

V

I

I

V

I

-

G

-

Y

-

T

-

K

-

E

-

N

-

P

K

R

P

P

Y

L

K

E

E

N

P

L

E

E

K

K

K

G

E

V

I

K

S

A

L

T

A

F

V

V

A

E

P

D

F

I

R

R

V

W

H

L

S

R

S

C

D

D

P

-

M

-

L

-

K

-

I

I

K
|
K

S

S

T

L

N

N

Y

L

S

L

R

G

N

A

I

V

I

L

A

A

K

K

R

Q

Y

E

A

A

L

H

E

E

K

K

G

G

Y

C

D

Y

D

E

A

A

L

I

F

-

L

L

N

H

E

R

N

N

G

N

E

T

V

V

T

T

E
|
E

T

G

S

S

S
|
S

A
x
S

N
|
N

I

V

F

F

W

G

I

I

K

K

G

D

R

G

F

I

L

L

Y

Y

T

T

P

H

S

P

V

A

D

N

C

N

G

M

L

I

L
|
L

P

K

G
|
G

V
x
I

T
|
T

R

R

S

D

V

V

I

V

L

I

K

A

K

C

A

A

K

N

D

E

E

I

G

N

F

M

A

P

V

V

V

K

E

E

G

I

R

P

F

F

Y

T

L

T

E

H

D

E

V

A

R

L

K

K

A

M

D

D

Y

E

I

L

F

F

V

V

A

T

N

S

A
x
T

L

T

N

S

G

E

I

I

I

T

R

P

V

V

S

I

K

E

I

I

E

D

active site: Y31 (≠ F28), V33 (≠ T30), K145 (= K139), E177 (= E172), L201 (= L196)
binding d-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-n,o-cycloserylamide: Y31 (≠ F28), V33 (≠ T30), R50 (= R47), R98 (vs. gap), H100 (vs. gap), K145 (= K139), E177 (= E172), S180 (= S175), S181 (≠ A176), N182 (= N177), L201 (= L196), G203 (= G198), I204 (≠ V199), T205 (= T200), T241 (≠ A236)

1daaA Crystallographic structure of d-amino acid aminotransferase complexed with pyridoxal-5'-phosphate (see paper)
30% identity, 92% coverage: 19:247/249 of query aligns to 22:252/277 of 1daaA

query
sites
1daaA

R

R

S

G

V

Y

M

Q

Y

F

G

G

E

D

G

G

V

V

F
x
Y

E

E

T
x
V

F

V

R

K

-

V

Y

Y

R

N

G

G

R

E

L

M

P

F

K

T

H

-

I

V

D

N

Q

E

H

H

Y

I

R

D

R
|
R

L

L

I

Y

K

A

G

S

A

A

E

E

L

K

L

I

K

R

I

I

P

T

-

I

K

P

I

Y

T

T

K

K

E

D

D

K

Y

F

I

H

F

Q

Y

L

I

L

E

H

E

E

T

L

V

V

K

E

K

K

C

N

K

E

E

-

S

-

D

-

L

-

Y

-

V

-

K

-

T

-

V

-

L

-

L

L

S

N

E

T

G

G

N

H

I

I

Y

Y

F

F

P

Q

V

V

V

T

-

R

-

G

-

T

-

S

-

P

-

R

-

A

-

H

-

Q

-

F

P

P

Y

E

K

N

S

T

H

V

L

K

L

P

V

V

I

I

V

I

-

G

-

Y

-

T

-

K

-

E

-

N

-

P

K

R

P

P

Y

L

K

E

E

N

P

L

E

E

K

K

K

G

E

V

I

K

S

A

L

T

A

F

V

V

A

E

P

D

F

I

R

R

V

W

H

L

S

R

S

C

D

D

P

-

M

-

L

-

K

-

I

I

K
|
K

S

S

T

L

N

N

Y

L

S

L

R

G

N

A

I

V

I

L

A

A

K

K

R

Q

Y

E

A

A

L

H

E

E

K

K

G

G

Y

C

D

Y

D

E

A

A

L

I

F

-

L

L

N

H

E

R

N

N

G

N

E

T

V

V

T

T

E
|
E

T

G

S

S

S
|
S

A
x
S

N

N

I

V

F

F

W

G

I

I

K

K

G

D

R

G

F

I

L

L

Y

Y

T

T

P

H

S

P

V

A

D

N

C

N

G

M

L

I

L
|
L

P

K

G
|
G

V
x
I

T
|
T

R

R

S

D

V

V

I

V

L

I

K

A

K

C

A

A

K

N

D

E

E

I

G

N

F

M

A

P

V

V

V

K

E

E

G

I

R

P

F

F

Y

T

L

T

E

H

D

E

V

A

R

L

K

K

A

M

D

D

Y

E

I

L

F

F

V

V

A

T

N
x
S

A
x
T

L

T

N

S

G

E

I

I

I

T

R

P

V

V

S

I

K

E

I

I

E

D

active site: Y31 (≠ F28), V33 (≠ T30), K145 (= K139), E177 (= E172), L201 (= L196)
binding pyridoxal-5'-phosphate: R50 (= R47), K145 (= K139), E177 (= E172), S180 (= S175), S181 (≠ A176), L201 (= L196), G203 (= G198), I204 (≠ V199), T205 (= T200), S240 (≠ N235), T241 (≠ A236)

3cswC Crystal structure of a putative branched-chain amino acid aminotransferase (tm0831) from thermotoga maritima at 2.15 a resolution
27% identity, 89% coverage: 26:246/249 of query aligns to 31:240/275 of 3cswC

query
sites
3cswC

G

A

V

V

F
x
Y

E

E

T

T

F

L

R

R

Y

T

R

Y

G

S

R

R

L

A

P

P

K

F

H

A

I

A

D

Y

Q

K

H

H

Y

Y

R

T

R
|
R

L

L

I

K

K

R

G

S

A

A

E

D

L

F

K

N

I

L

P

P

K

-

I

-

T

-

K

-

E

-

D

-

Y

L

I

S

F

L

Y

S

I

F

E

D

E

E

T

F

V

T

K

K

K

V

C

L

K

K

E

A

S

G

D

A

D

D

L

E

Y

F

V

K

K

Q

T

E

V

V

L

R

L

I

S

-

E

-

G

-

N

K

I

V

Y

Y

F

-

P

-

V

L

V

F

P

P

Y

D

K

S

S

G

H

E

L

V

L

L

V

F

I

V

V

F

K

S

P

P

Y

L

K

N

E

I

P

P

E

D

-

L

K

E

K

T

E

G

I

V

S

E

L

V

A

K

V

I

A

S

P

N

F

V

R

R

V

R

-

I

-

P

-

D

H

L

S

S

S

T

D

P

P

P

M

A

L

L

K
|
K

I

I

K

T

S

G

T

R

N

T

Y

-

S

-

R

-

N

D

I

I

I

V

A

L

K

A

R

R

Y

R

A

E

L

I

E

V

K

D

G

C

Y

Y

D

D

D

-

A

V

L

I

F

L

L

L

N

G

E

L

N

N

G

G

E

Q

V

V

T

C

E
|
E

T

G

S

S

S
x
F

A
x
S

N

N

I

V

F

F

W

L

I

V

K

K

G

E

R

G

F

K

L

L

Y

I

T

T

P

P

S

S

V

L

D

D

C

S

G

G

L

I

L
|
L

P

D

G
|
G

V
x
I

T
|
T

R

R

S

E

V

N

I

V

L

I

K

K

K

L

A

A

K

K

D

S

E

L

G

E

F

I

A

P

V

V

V

E

E

E

G

R

R

V

F

V

Y

W

L

V

E

W

D

E

V

L

R

F

K

E

A

A

D

D

Y

E

I

M

F

F

V

L

A

T

N

H

A
x
T

L

S

N

A

G

G

I

V

R

P

V

V

S

R

K

R

I

L

active site: Y33 (≠ F28), K135 (= K137), E166 (= E172), L190 (= L196)
binding pyridoxal-5'-phosphate: R52 (= R47), K135 (= K137), E166 (= E172), F169 (≠ S175), S170 (≠ A176), L190 (= L196), G192 (= G198), I193 (≠ V199), T194 (= T200), T230 (≠ A236)

7z79B Crystal structure of aminotransferase-like protein from variovorax paradoxus
26% identity, 98% coverage: 4:247/249 of query aligns to 17:273/306 of 7z79B

query
sites
7z79B

I

L

V

V

T

Y

V

V

N

N

G

G

K

Q

-

F

-

V

-

P

-

R

-

H

K

Q

L

A

I

V

D

V

T

S

R

V

F

F

L

D

R

A

S

G

V

Y

M

V

Y

C

G

G

E

D

G

G

V

V

F
x
W

E

E

T

G

F

V

R

R

Y

L

-

V

R

D

G

G

R

R

L

I

P

V

K

S

H

-

I

F

D

D

Q

A

H

H

Y

I

R

D

R
|
R

L

M

I

Y

K

E

G

G

A

A

E

K

L

S

L

I

K

A

I

L

P

D

-

I

K

G

I

M

T

T

K

R

E

A

D

Q

Y

T

I

K

F

Q

Y

V

I

V

E

V

E

D

T

T

V

F

K

L

K

R

C

N

K

G

E

M

S

R

D

D

D

G

L

A

Y

H

V

A

K

R

T

-

V

L

L

M

L

V

S

T

E

R

G

G

N

V

I

K

Y

K

F

T

P

P

-

N

-

Q

-

D

-

P

-

R

-

F

V

I

P

G

Y

G

K

A

S

T

H

V

L

V

L

C

V

V

I

A

V

E

K

H

P

K

Y

V

K

V

E

T

P

P

E

E

K

A

K

K

E

R

I

N

S

G

L

L

A

K

V

L

A

F

P

T

F

S

R

T

V

L

H

R

S

C

S

S

D

G

P

P

-

D

M

V

L

F

K

D

I

L

K

R

S

L

T
x
N

N

S

Y

H

S

S

R

R

-

L

N

N

I

L

I

I

-

Q

A

A

K

L

R

I

Y

Q

A

A

L

I

E

Q

K

A

G

G

Y

A

D

D

D

E

A

A

L

L

F

M

L

L

N

D

E

P

N

N

G

G

E

F

V

V

T

S

E

S

T

C

S

N

S
|
S

A
x
T

N

N

I

F

F

F

W

A

I

V

K

R

G

N

R

G

F

A

L

L

Y

W

T

T

P

S

S

S

V

G

D

R

-

Y

C

C

G

-

L

-

L
x
F

P

N

G
|
G

V
x
I

T
|
T

R

R

S

A

V

T

I

V

L

V

K

R

K

L

A

A

K

R

D

E

E

A

G

G

F

I

A

P

V

V

V

H

E

E

G

G

R

D

F

F

Y

T

L

L

E

A

D

E

V

V

R

Y

K

A

A

A

D

D

Y

E

I

A

F

F

V

V

A

T

N
x
G

A
x
T

L

L

N

A

G

G

I

L

I

T

R

P

V

V

S

S

K

S

I

V

E

D

binding pyridoxal-5'-phosphate: W46 (≠ F28), R65 (= R47), N166 (≠ T141), S202 (= S175), T203 (≠ A176), F222 (≠ L196), G224 (= G198), I225 (≠ V199), T226 (= T200), G261 (≠ N235), T262 (≠ A236)

1a0gB L201a mutant of d-amino acid aminotransferase complexed with pyridoxamine-5'-phosphate (see paper)
29% identity, 92% coverage: 19:247/249 of query aligns to 22:252/282 of 1a0gB

query
sites
1a0gB

R

R

S

G

V

Y

M

Q

Y

F

G

G

E

D

G

G

V

V

F
x
Y

E

E

T
x
V

F

V

R

K

-

V

Y

Y

R

N

G

G

R

E

L

M

P

F

K

T

H

-

I

V

D

N

Q

E

H

H

Y

I

R

D

R
|
R

L

L

I

Y

K

A

G

S

A

A

E

E

L

K

L

I

K

R

I

I

P

T

-

I

K

P

I

Y

T

T

K

K

E

D

D

K

Y

F

I

H

F

Q

Y

L

I

L

E

H

E

E

T

L

V

V

K

E

K

K

C

N

K

E

E

-

S

-

D

-

L

-

Y

-

V

-

K

-

T

-

V

-

L

-

L

L

S

N

E

T

G

G

N

H

I

I

Y

Y

F

F

P

Q

V

V

V

T

-

R

-

G

-

T

-

S

-

P

-

R

-

A

-

H

-

Q

-

F

P

P

Y

E

K

N

S

T

H

V

L

K

L

P

V

V

I

I

V

I

-

G

-

Y

-

T

-

K

-

E

-

N

-

P

K

R

P

P

Y

L

K

E

E

N

P

L

E

E

K

K

K

G

E

V

I

K

S

A

L

T

A

F

V

V

A

E

P

D

F

I

R

R

V

W

H

L

S

R

S

C

D

D

P

-

M

-

L

-

K

-

I

I

K
|
K

S

S

T

L

N

N

Y

L

S

L

R

G

N

A

I

V

I

L

A

A

K

K

R

Q

Y

E

A

A

L

H

E

E

K

K

G

G

Y

C

D

Y

D

E

A

A

L

I

F

-

L

L

N

H

E

R

N

N

G

N

E

T

V

V

T

T

E
|
E

T

G

S

S

S
|
S

A
x
S

N

N

I

V

F

F

W

G

I

I

K

K

G

D

R

G

F

I

L

L

Y

Y

T

T

P

H

S

P

V

A

D

N

C

N

G

M

L

I

L
x
A

P

K

G
|
G

V
x
I

T
|
T

R

R

S

D

V

V

I

V

L

I

K

A

K

C

A

A

K

N

D

E

E

I

G

N

F

M

A

P

V

V

V

K

E

E

G

I

R

P

F

F

Y

T

L

T

E

H

D

E

V

A

R

L

K

K

A

M

D

D

Y

E

I

L

F

F

V

V

A

T

N
x
S

A
x
T

L

T

N

S

G

E

I

I

I

T

R

P

V

V

S

I

K

E

I

I

E

D

active site: Y31 (≠ F28), V33 (≠ T30), K145 (= K139), E177 (= E172), A201 (≠ L196)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R50 (= R47), K145 (= K139), E177 (= E172), S180 (= S175), S181 (≠ A176), G203 (= G198), I204 (≠ V199), T205 (= T200), S240 (≠ N235), T241 (≠ A236)

1iyeA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
23% identity, 92% coverage: 19:247/249 of query aligns to 24:265/304 of 1iyeA

query
sites
1iyeA

R

H

S

A

V

L

M

H

Y

Y

G

G

E

T

G

S

V

V

F
|
F

E

E

T
x
G

F

I

R

R

-

C

-

Y

-

D

-

S

Y

H

R

K

G

G

R

P

L

V

P

V

K

F

H

R

I

H

D

R

Q

E

H

H

Y

M

R

Q

R
|
R

L

L

I

H

K

D

G

S

A

A

E

K

L

I

L

Y

K

R

I

F

P

P

K

V

I

S

T

Q

K

S

E

I

D

D

Y

E

I

L

F

M

Y

E

I

A

E

C

E

R

T

D

V

V

K

I

K

R

C

K

K

N

E

N

S

L

D

T

D

S

L

A

Y
|
Y

V

I

K

R

T

P

V

L

L

I

L

F

S

V

E

-

G

G

N

D

I

V

Y

G

F

M

P

G

V

V

V

N

P

P

-

P

-

A

-

G

Y

Y

K

S

S

T

H

D

L

V

L

I

V

I

I

A

V

A

K

F

P

P

Y

W

-

G

-

A

-
x
Y

-

L

-

G

K

A

E

E

P

A

E

L

K

E

K

Q

E

G

I

I

S

D

L

A

A

M

V

V

A

S

P

S

F

W

R

N

V

R

H

A

S

A

S

P

D

N

-

T

-

I

P

P

M

T

L

A

K

A

I
x
K

K
x
A

S

G

T

G

N

N

Y
|
Y

S

L

R

S

N

S

I

L

A

V

K

G

R

S

Y

E

A

A

L

R

E

R

K

H

G

G

Y

Y

D

Q

D

E

A

G

L

I

F

A

L

L

N

D

E

V

N

N

G

G

E

Y

V

I

T

S

E
|
E

T

G

S

A

S
x
G

A
x
E

N
|
N

I

L

F

F

W

E

I

V

K

K

G

D

R

G

F

V

L

L

Y

F

T

T

P

P

S

P

V

F

D

T

C

S

G

S

L

A

L
|
L

P

P

G
|
G

V
x
I

T
|
T

R

R

S

D

V

A

I

I

L

I

K

K

K

L

A

A

K

K

D

E

E

L

G

G

F

I

A

E

V

V

V

R

E

E

G

Q

R

V

F

L

Y

S

L

R

E

E

D

S

V

L

R

Y

K

L

A

A

D

D

Y

E

I

V

F

F

V

M

A

S

N
x
G

A
x
T

L
x
A

N

A

G

E

I

I

I

T

R

P

V

V

S

R

K

S

I

V

E

D

active site: F33 (= F28), G35 (≠ T30), K156 (≠ I138), A157 (≠ K139), E190 (= E172), L214 (= L196)
binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: R56 (= R47), Y92 (= Y83), Y126 (vs. gap), K156 (≠ I138), Y161 (= Y143), E190 (= E172), G193 (≠ S175), E194 (≠ A176), N195 (= N177), L214 (= L196), G216 (= G198), I217 (≠ V199), T218 (= T200), G253 (≠ N235), T254 (≠ A236), A255 (≠ L237)

1iydA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
23% identity, 92% coverage: 19:247/249 of query aligns to 24:265/304 of 1iydA

query
sites
1iydA

R

H

S

A

V

L

M

H

Y

Y

G

G

E

T

G

S

V

V

F
|
F

E

E

T
x
G

F

I

R

R

-

C

-

Y

-

D

-

S

Y

H

R

K

G

G

R

P

L

V

P

V

K

F

H

R

I

H

D

R

Q

E

H

H

Y

M

R

Q

R
|
R

L

L

I

H

K

D

G

S

A

A

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active site: F33 (= F28), G35 (≠ T30), K156 (≠ I138), A157 (≠ K139), E190 (= E172), L214 (= L196)
binding glutaric acid: Y92 (= Y83), Y126 (vs. gap), A255 (≠ L237)
binding pyridoxal-5'-phosphate: R56 (= R47), K156 (≠ I138), Y161 (= Y143), E190 (= E172), G193 (≠ S175), E194 (≠ A176), L214 (= L196), G216 (= G198), I217 (≠ V199), T218 (= T200), T254 (≠ A236)

Query Sequence

>WP_012676469.1 NCBI__GCF_000021565.1:WP_012676469.1
MDYIVTVNGKKLIDTRFLRSVMYGEGVFETFRYRGRLPKHIDQHYRRLIKGAELLKIPKI
TKEDYIFYIEETVKKCKESDDLYVKTVLLSEGNIYFPVVPYKSHLLVIVKPYKEPEKKEI
SLAVAPFRVHSSDPMLKIKSTNYSRNIIAKRYALEKGYDDALFLNENGEVTETSSANIFW
IKGRFLYTPSVDCGLLPGVTRSVILKKAKDEGFAVVEGRFYLEDVRKADYIFVANALNGI
IRVSKIEIL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory