SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012676481.1 NCBI__GCF_000021565.1:WP_012676481.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 1 hits to proteins with known functional sites (download)

3al0C Crystal structure of the glutamine transamidosome from thermotoga maritima in the glutamylation state. (see paper)
32% identity, 95% coverage: 2:92/96 of query aligns to 2:92/564 of 3al0C

query
sites
3al0C

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Sites not aligning to the query:

active site: 110, 335
binding o5'-(l-glutamyl-sulfamoyl)-adenosine: 106, 108, 109, 118, 122, 142, 276, 294, 295, 297, 298, 324, 325, 333
binding : 144, 145, 148, 208, 213, 252, 255, 266, 269, 270, 276, 297, 298, 299, 300, 301, 304, 330, 332, 363, 364, 365, 370, 387, 389, 391, 392, 397, 400, 407, 446, 447, 453, 457, 509, 520, 524, 527, 535, 536, 538, 539

Query Sequence

>WP_012676481.1 NCBI__GCF_000021565.1:WP_012676481.1
MIDRQTVEKVAQLAKLRLTEEEVELFSKQLSDILGFIEKLEELDTENILPFYEMIDQEAP
LREDIPEEGLTNDDALKNAPQSEDGFFVVPRVVSAE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory