SitesBLAST – Find functional sites

 

SitesBLAST

Comparing WP_012676497.1 NCBI__GCF_000021565.1:WP_012676497.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 6 hits to proteins with known functional sites (download)

Q58667 Methanogen homoaconitase small subunit; HACN; Homoaconitate hydratase; EC 4.2.1.114 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
55% identity, 98% coverage: 1:161/165 of query aligns to 1:159/170 of Q58667

query
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Q58667
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R

2pkpA Crystal structure of 3-isopropylmalate dehydratase (leud)from methhanocaldococcus jannaschii dsm2661 (mj1271) (see paper)
55% identity, 98% coverage: 1:161/165 of query aligns to 1:159/167 of 2pkpA

query
sites
2pkpA
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M
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R

O14289 3-isopropylmalate dehydratase; Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase; EC 4.2.1.33 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
41% identity, 57% coverage: 13:106/165 of query aligns to 554:658/758 of O14289

query
sites
O14289
D
 
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Sites not aligning to the query:

P9WK95 3-isopropylmalate dehydratase small subunit; Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase; EC 4.2.1.33 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
38% identity, 53% coverage: 13:100/165 of query aligns to 18:110/198 of P9WK95

query
sites
P9WK95
D
 
N
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V
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D
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T
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D
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Q
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I
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I
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P
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L

Sites not aligning to the query:

P09339 Aconitate hydratase A; ACN; Aconitase; Aconitate/2-methylaconitate hydratase; Iron-responsive protein-like; IRP-like; RNA-binding protein; EC 4.2.1.3; EC 4.2.1.- from Bacillus subtilis (strain 168) (see 2 papers)
31% identity, 47% coverage: 50:126/165 of query aligns to 777:857/909 of P09339

query
sites
P09339
K
 
K
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T
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V

Sites not aligning to the query:

D9X0I3 Aconitate hydratase A; ACN; Aconitase; EC 4.2.1.3 from Streptomyces viridochromogenes (strain DSM 40736 / JCM 4977 / BCRC 1201 / Tue 494) (see paper)
37% identity, 38% coverage: 54:115/165 of query aligns to 805:866/931 of D9X0I3

query
sites
D9X0I3
I
 
V
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A
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E
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S
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G

Sites not aligning to the query:

Query Sequence

>WP_012676497.1 NCBI__GCF_000021565.1:WP_012676497.1
MVIEGKVWKFGDDINTDEIIPARYLITTDPKELAKHVMEDADPEFPNKVKPGDIIVAGKN
FGCGSSREHAPLALKGAEIGAIIAESFARIFYRNAINLGLPIIESPEAARDAEEGDIIQI
DMDKGIIRNVTKGKEYTFKPLPESLRKVFEAGGLMEYAKDKLKGE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory