SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012676796.1 NCBI__GCF_000021565.1:WP_012676796.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P09342 Acetolactate synthase 1, chloroplastic; ALS I; Acetohydroxy-acid synthase I; Acetolactate synthase I; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see 2 papers)
48% identity, 99% coverage: 4:578/581 of query aligns to 94:667/667 of P09342

query
sites
P09342

K

R

R

K

G

G

A

S

D

D

I

V

V

L

I

V

D

E

V

A

L

L

L

E

E

R

E

E

G

G

V

V

D

T

T

D

V

V

F

F

G

A

L

Y

P

P

G

G

A

A

I

S

M

M

E

E

V

I

Y

H

D

Q

A

A

L

L

F

T

D

R

A

S

P

S

L

I

-

I

R

R

N

N

I

V

L

L

A

P

R

R

H

H

E

E

Q

Q

A

G

A

V

H

F

M

A

A

A

D

E

G

G

Y

Y

A

A

R

R

A

A

T

T

G

G

K

F

V

P

G

G

V

V

V
x
C

L

I

A

A

T

T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

G

G

I

L

A

A

T

D

A

A

Y

L

M

L

D

D

S

S

V

V

P

P

L

I

V

V

A

A

I

I

T

T

G

G

Q

Q

V

V

P
|
P

T

R

S

R

Y

M

I

I

G

G

T

T

D

D

A

A

F

F

Q

Q

E

E

A

T

D

P

V

I

V

V

G

E

I

V

T

T

R

R

P

S

I

I

T

T

K

K

H

H

N

N

F

Y

L

L

V

V

T

M

D

D

I

V

K

E

D

D

L

I

A

P

L

R

I

V

L

V

R

R

E

E

A

A

F

F

Y

F

L

L

A

A

R

R

T

S

G

G

R

R

P

P

G

G

P

P

V

I

L

L

V

I

D

D

I

V

P

P

K

K

D

D

I

I

T

Q

Q

Q

E

L

Y

V

-

I

-

P

D

D

Y

W

K

D

M

Q

P

P

T

M

E

R

K

-

D

-

V

-

L

-

D

-

A

-

L

L

P

P

G

G

Y

Y

K

M

P

S

H

R

Y

L

E

P

G

K

N

L

P

P

F

N

Q

E

-

M

-

L

I

L

K

E

K

Q

A

I

A

V

E

R

L

L

I

I

R

S

K

E

A

S

K

K

R

K

P

P

V

V

L

L

Y

Y

V

V

G

G

T
x
C

V

-

I

-

S

S

G

Q

A

S

S

S

E

E

E

D

L

L

R

R

E

R

L

F

A

V

E

E

L

L

T

T

R

G

I

I

P

P

V

V

T

A

T

S

T

T

N

L

M

M

A

G

K

L

G

G

A

A

F

F

D

P

E

T

T

G

H

D

P

E

L

L

A

S

L

L

H

S

M

M

L

L

G

G

M

M

H

H

G

G

T

T

Y

V

Y

Y

A

A

N

N

M

Y

A

A

V

V

Y

D

H

S

S

S

D

D

L

L

I

L

A

A

V

F

G

G

A

V

R

R

F

F

D

D

R

R

V

V

T

T

G

G

K

K

I

L

Q

E

E

A

F

F

A

A

P

S

E

R

A

A

K

K

I

I

I

V

H

H

I

I

D

D

I

I

D

D

P

S

A

A

S

E

I

I

S

G

K

K

N

N

I

K

T

Q

V

P

D

H

V

V

P

S

I

I

V

C

G

A

D

D

V

I

K

K

V

L

V

A

L

L

G

Q

K

G

L

L

L

N

K

S

E

I

L

L

K

E

K

S

K

K

P

E

I

G

E

K

W

L

V

K

K

L

A

D

R

F

E

S

S

A

W

W

L

R

K

Q

Q

E

I

L

E

T

K

E

W

Q

K

K

E

V

K

K

H

H

P

P

L

L

T

N

Y

F

D

K

R

T

T

F

S

G

K

D

I

A

I

I

K

P

P

P

Q

Q

Y

Y

V

A

I

I

E

Q

E

V

I

L

Y

D

K

E

I

L

T

T

D

N

G

G

E

N

A

A

L

I

I

I

T

S

A

T

G

G

V

V

G

G

Q

Q

H

H

Q

Q

M

M

W

W

A

A

M

Q

F

Y

Y

Y

K

K

Y

Y

R

R

Y

K

P

P

R

R

Q

Q

F

W

L

L

N

T

S

S

G

G

L

L

G

G

T

A

M

M

G

G

Y

F

G

G

F

L

P

P

A

A

V

I

G

G

A

A

K

A

I

V

G

G

N

R

P

P

D

D

K

E

T

V

V

V

F

V

A

D

I

I

E

D

G

G

D

D

G

G

S

S

F

F

V

I

M

M

N

N

M

V

Q

Q

D

E

V

L

I

A

T

T

A

I

V

K

Q

V

Y

E

R

N

I

L

P

P

V

V

K

K

I

I

A

M

I

L

N

N

E

Q

F

H

L

L

G

G

M

M

V

V

R

V

Q

Q

W

W

Q

E

Q

D

L

R

F

F

Y

Y

D

K

S

A

R

N

Y

R

S

A

S

H

V

T

C

Y

L

L

A

G

-

N

-

P

-

S

-

N

-

E

-

A

-

E

V

I

H

F

P

P

D

N

F

M

V

L

K

K

L

F

A

A

E

E

S

A

M

C

G

G

A

V

V

P

G

A

L

A

R

R

A

V

T

T

K

H

P

R

E

D

V

L

R

R

P

A

V

A

L

I

E

Q

K

K

A

M

M

L

K

D

I

-

N

T

D

P

R

G

P

P

V

Y

I

L

M

L

D

D

F

V

V

I

V

V

D

P

R

H

E

Q

E

E

N

H

V

V

L

L

P

P

M

M

V

I

P

P

A

S

G

G

K

G

S

A

Y

F

R

K

E

D

M

V

I

I

V

T

S

-

P

-

K

-

Q

-

K

E

G

G

E

D

A

G

E

R

T

S

M

S

Y

Y

C161 (≠ V70) modified: Disulfide link with 307
P194 (= P103) mutation to Q: In C3; highly resistant to sulfonylurea herbicides.
C307 (≠ T218) modified: Disulfide link with 161

3ea4A Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron-ester (see paper)
49% identity, 97% coverage: 4:565/581 of query aligns to 11:575/582 of 3ea4A

query
sites
3ea4A

K

R

R

K

G

G

A

A

D

D

I

I

V

L

I

V

D

E

V

A

L

L

L

E

E

R

E

Q

G

G

V

V

D

E

T

T

V

V

F

F

G

A

L
x
Y

P

P

G
|
G

A
|
A

I
x
S

M

M

E

E

V

I

Y

H

D

Q

A

A

L

L

F

T

-

R

D

S

A

S

P

S

L

I

R

R

N

N

I

V

L

L

A

P

R

R

H

H

E
|
E

Q

Q

A

G

A

V

H

F

M

A

A

A

D

E

G

G

Y

Y

A

A

R

R

A

S

T

S

G

G

K

K

V

P

G

G

V

I

V

C

L

I

A

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

G

G

I

L

A

A

T

D

A

A

Y

L

M

L

D

D

S

S

V

V

P

P

L

L

V

V

A

A

I

I

T

T

G

G

Q

Q

V

V

P

P

T

R

S

R

Y

M

I

I

G

G

T

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

A

T

D

P

V

I

V

V

G

E

I

V

T

T

R

R

P

S

I

I

T

T

K

K

H

H

N

N

F

Y

L

L

V

V

T

M

D

D

I

V

K

E

D

D

L

I

A

P

L

R

I

I

L

I

R

E

E

E

A

A

F

F

Y

F

L

L

A

A

R

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

V

V

D

D

I

V

P

P

K
|
K

D

D

I

I

T

Q

Q

Q

E

-

Y

-

D

-

Y

L

K

A

M

I

P

P

T

N

E

W

K

E

D

Q

V

A

L

M

D

-

A

R

L

L

P

P

G

G

Y

Y

K

M

P

S

H

R

Y

M

E

P

G

K

N

P

P

P

-

E

-

D

F

S

Q

H

I

L

K

E

K

Q

A

I

A

V

E

R

L

L

I

I

R

S

K

E

A

S

K

K

R

K

P

P

V

V

L

L

Y

Y

V

V

G
|
G

T

C

V

L

I

N

S

S

G

-

A

-

S

S

E

D

E

E

L

L

R

G

E

R

L

F

A

V

E

E

L

L

T

T

R

G

I

I

P

P

V

V

T

A

T

T

T
|
T

N
x
L

M
|
M

A

G

K

L

G

G

A

S

F

Y

D

P

E

C

T

D

H

D

P

E

L

L

A

S

L

L

H

H

M

M

L
|
L

G
|
G

M
|
M

H
|
H

G

G

T

T

Y

V

Y

Y

A

A

N

N

M

Y

A

A

V

V

Y

E

H

H

S

S

D

D

L

L

I

L

A

A

V

F

G
|
G

A

V

R
|
R

F

F

D
|
D

R
|
R

V
|
V

T

T

G

G

K

K

I

L

Q

E

E

A

F

F

A

A

P

S

E

R

A

A

K

K

I

I

I

V

H

H

I

I

D
|
D

I
|
I

D

D

P

S

A

A

S

E

I

I

S

G

K

K

N

N

I

K

T

T

V

P

D

H

V

V

P

S

I

V

V

C

G
|
G

D
|
D

V
|
V

K

K

V

L

V

A

L

L

G

Q

K

G

L

M

L

N

K

K

E

V

L

L

K

E

K

N

K

R

P

A

I

E

E

E

W

L

V

K

K

L

A

D

R

F

E

G

S

V

W

W

L

R

K

N

Q

E

I

L

E

N

K

V

W

Q

K

K

E

Q

K

K

H

F

P

P

L

L

T

S

Y

F

D

K

R

T

T

F

S

G

K

E

I

A

I

I

K

P

P

P

Q

Q

Y

Y

V

A

I

I

E

K

E

V

I

L

Y

D

K

E

I

L

T

T

D

D

G

G

E

K

A

A

L

I

I

I

T

S

A

T

G

G

V
|
V

G
|
G

Q
|
Q

H
|
H

Q

Q

M
|
M

W

W

A

A

M

Q

F

F

Y

Y

K

N

Y

Y

R

K

Y

K

P

P

R

R

Q

Q

F

W

L

L

N

S

S

S

G
|
G

G

G

L

L

G
|
G

T

A

M
|
M

G

G

Y

F

G

G

F

L

P

P

A

A

V

I

G

G

A

A

K

S

I

V

G

A

N

N

P

P

D

D

K

A

T

I

V

V

F

V

A

D

I

I

E

D

G
|
G

D
|
D

G
|
G

S
|
S

F

F

V

I

M

M

N

N

M

V

Q

Q

D

E

V

L

I

A

T

T

A

I

V

R

Q

V

Y

E

R

N

I

L

P

P

V

V

K

K

I

V

A

L

I

L

N

N

N
|
N

E

Q

F
x
H

L
|
L

G
|
G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

Q

D

L

R

F

F

Y

Y

D

K

S

A

R

N

Y

R

S

A

S

H

V

T

C

F

L

L

A

G

-

D

-

P

-

A

-

Q

-

E

-

D

-

E

V

I

H

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

S

A

M

C

G

G

A

I

V

P

G

A

L

A

R

R

A

V

T

T

K

K

P

K

E

A

E

D

V

L

R

R

P

E

V

A

L

I

E

Q

K

-

A

T

M

M

K

L

I

D

N

T

D

P

R

G

P

P

V

Y

I

L

M

L

D

D

F

V

V

I

V

C

D

P

R
x
H

E

Q

E

E

N

H

V

V

L

L

P

P

M

M

V

I

P

P

A
x
S

G

G

K

G

S

T

Y

F

R

N

E

D

M

V

I

I

active site: Y32 (≠ L25), G34 (= G27), G35 (= G28), A36 (= A29), S37 (≠ I30), E58 (= E50), T81 (= T73), F120 (= F112), Q121 (= Q113), E122 (= E114), K170 (= K162), M265 (= M261), V292 (= V288), V399 (= V395), G425 (= G421), M427 (= M423), D452 (= D448), N479 (= N475), H481 (≠ F477), L482 (= L478), M484 (= M480), V485 (= V481), W488 (= W484), H557 (≠ R547)
binding methyl 2-{[(4-methylpyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: D290 (= D286), R291 (= R287), W488 (= W484), S567 (≠ A557)
binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (= R152), G221 (= G215), G222 (= G216), G223 (= G217), T245 (= T241), L246 (≠ N242), M247 (= M243), L263 (= L259), G264 (= G260), M265 (= M261), H266 (= H262), G285 (= G281), R287 (= R283), D289 (= D285), R291 (= R287), D309 (= D305), I310 (= I306), G327 (= G323), D328 (= D324), V329 (= V325), M404 (= M400), G422 (= G418)
binding magnesium ion: D452 (= D448), N479 (= N475), H481 (≠ F477)
binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (= V395), G400 (= G396), Q401 (= Q397), H402 (= H398), M427 (= M423), G451 (= G447), D452 (= D448), G453 (= G449), S454 (= S450), N479 (= N475), H481 (≠ F477), L482 (= L478), G483 (= G479), M484 (= M480), V485 (= V481)

3e9yA Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron (see paper)
49% identity, 97% coverage: 4:565/581 of query aligns to 11:575/582 of 3e9yA

query
sites
3e9yA

K

R

R

K

G

G

A

A

D

D

I

I

V

L

I

V

D

E

V

A

L

L

L

E

E

R

E

Q

G

G

V

V

D

E

T

T

V

V

F

F

G

A

L
x
Y

P

P

G
|
G

A
|
A

I
x
S

M

M

E

E

V

I

Y

H

D

Q

A

A

L

L

F

T

-

R

D

S

A

S

P

S

L

I

R

R

N

N

I

V

L

L

A

P

R

R

H

H

E
|
E

Q

Q

A

G

A

V

H

F

M

A

A

A

D

E

G

G

Y

Y

A

A

R

R

A

S

T

S

G

G

K

K

V

P

G

G

V

I

V

C

L

I

A

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

G

G

I

L

A

A

T

D

A

A

Y

L

M

L

D

D

S

S

V

V

P

P

L

L

V

V

A

A

I

I

T

T

G

G

Q

Q

V

V

P

P

T

R

S

R

Y

M

I

I

G

G

T

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

A

T

D

P

V

I

V

V

G

E

I

V

T

T

R

R

P

S

I

I

T

T

K

K

H

H

N

N

F

Y

L

L

V

V

T

M

D

D

I

V

K

E

D

D

L

I

A

P

L

R

I

I

L

I

R

E

E

E

A

A

F

F

Y

F

L

L

A

A

R

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

V

V

D

D

I

V

P

P

K
|
K

D

D

I

I

T

Q

Q

Q

E

-

Y

-

D

-

Y

L

K

A

M

I

P

P

T

N

E

W

K

E

D

Q

V

A

L

M

D

-

A

R

L

L

P

P

G

G

Y

Y

K

M

P

S

H

R

Y

M

E

P

G

K

N

P

P

P

-

E

-

D

F

S

Q

H

I

L

K

E

K

Q

A

I

A

V

E

R

L

L

I

I

R

S

K

E

A

S

K

K

R

K

P

P

V

V

L

L

Y

Y

V

V

G
|
G

T

C

V

L

I

N

S

S

G

-

A

-

S

S

E

D

E

E

L

L

R

G

E

R

L

F

A

V

E

E

L

L

T

T

R

G

I

I

P

P

V

V

T

A

T

T

T
|
T

N
x
L

M
|
M

A

G

K

L

G

G

A

S

F

Y

D

P

E

C

T

D

H

D

P

E

L

L

A

S

L

L

H

H

M

M

L
|
L

G

G

M
|
M

H

H

G

G

T

T

Y

V

Y

Y

A

A

N

N

M

Y

A

A

V

V

Y

E

H

H

S

S

D

D

L

L

I

L

A

A

V

F

G
|
G

A

V

R
|
R

F

F

D
|
D

R
|
R

V
|
V

T

T

G

G

K

K

I

L

Q

E

E

A

F

F

A

A

P

S

E

R

A

A

K

K

I

I

I

V

H

H

I

I

D
|
D

I
|
I

D

D

P

S

A

A

S

E

I

I

S

G

K

K

N

N

I

K

T

T

V

P

D

H

V

V

P

S

I

V

V

C

G
|
G

D
|
D

V
|
V

K

K

V

L

V

A

L

L

G

Q

K

G

L

M

L

N

K

K

E

V

L

L

K

E

K

N

K

R

P

A

I

E

E

E

W

L

V

K

K

L

A

D

R

F

E

G

S

V

W

W

L

R

K

N

Q

E

I

L

E

N

K

V

W

Q

K

K

E

Q

K

K

H

F

P

P

L

L

T

S

Y

F

D

K

R

T

T

F

S

G

K

E

I

A

I

I

K

P

P

P

Q

Q

Y

Y

V

A

I

I

E

K

E

V

I

L

Y

D

K

E

I

L

T

T

D

D

G

G

E

K

A

A

L

I

I

I

T

S

A

T

G

G

V
|
V

G
|
G

Q
|
Q

H
|
H

Q

Q

M
|
M

W

W

A

A

M

Q

F

F

Y

Y

K

N

Y

Y

R

K

Y

K

P

P

R

R

Q

Q

F

W

L

L

N

S

S

S

G
|
G

G

G

L

L

G
|
G

T

A

M
|
M

G

G

Y

F

G

G

F

L

P

P

A

A

V

I

G

G

A

A

K

S

I

V

G

A

N

N

P

P

D

D

K

A

T

I

V

V

F

V

A

D

I

I

E

D

G
|
G

D
|
D

G
|
G

S
|
S

F

F

V

I

M

M

N

N

M

V

Q

Q

D

E

V

L

I

A

T

T

A

I

V

R

Q

V

Y

E

R

N

I

L

P

P

V

V

K

K

I

V

A

L

I

L

N

N

N
|
N

E

Q

F
x
H

L
|
L

G
|
G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

Q

D

L

R

F

F

Y

Y

D

K

S

A

R

N

Y

R

S

A

S

H

V

T

C

F

L

L

A

G

-

D

-

P

-

A

-

Q

-

E

-

D

-

E

V

I

H

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

S

A

M

C

G

G

A

I

V

P

G

A

L

A

R

R

A

V

T

T

K

K

P

K

E

A

E

D

V

L

R

R

P

E

V

A

L

I

E

Q

K

-

A

T

M

M

K

L

I

D

N

T

D

P

R

G

P

P

V

Y

I

L

M

L

D

D

F

V

V

I

V

C

D

P

R
x
H

E

Q

E

E

N

H

V

V

L

L

P

P

M

M

V

I

P

P

A
x
S

G

G

K

G

S

T

Y

F

R

N

E

D

M

V

I

I

active site: Y32 (≠ L25), G34 (= G27), G35 (= G28), A36 (= A29), S37 (≠ I30), E58 (= E50), T81 (= T73), F120 (= F112), Q121 (= Q113), E122 (= E114), K170 (= K162), M265 (= M261), V292 (= V288), V399 (= V395), G425 (= G421), M427 (= M423), D452 (= D448), N479 (= N475), H481 (≠ F477), L482 (= L478), M484 (= M480), V485 (= V481), W488 (= W484), H557 (≠ R547)
binding N-[(4-methylpyrimidin-2-yl)carbamoyl]-2-nitrobenzenesulfonamide: D290 (= D286), R291 (= R287), W488 (= W484), S567 (≠ A557)
binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (= R152), G221 (= G215), G222 (= G216), G223 (= G217), T245 (= T241), L246 (≠ N242), M247 (= M243), L263 (= L259), G285 (= G281), R287 (= R283), D289 (= D285), R291 (= R287), D309 (= D305), I310 (= I306), G327 (= G323), D328 (= D324), V329 (= V325), M404 (= M400), G422 (= G418)
binding magnesium ion: D452 (= D448), N479 (= N475), H481 (≠ F477)
binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (= V395), G400 (= G396), Q401 (= Q397), H402 (= H398), M427 (= M423), G451 (= G447), G453 (= G449), S454 (= S450), N479 (= N475), H481 (≠ F477), L482 (= L478), G483 (= G479), M484 (= M480), V485 (= V481)

P17597 Acetolactate synthase, chloroplastic; AtALS; Acetohydroxy-acid synthase; Protein CHLORSULFURON RESISTANT 1; EC 2.2.1.6 from Arabidopsis thaliana (Mouse-ear cress) (see 8 papers)
48% identity, 97% coverage: 4:565/581 of query aligns to 97:661/670 of P17597

query
sites
P17597

K

R

R

K

G

G

A

A

D

D

I

I

V

L

I

V

D

E

V

A

L

L

L

E

E

R

E

Q

G

G

V

V

D

E

T

T

V

V

F

F

G

A

L

Y

P

P

G

G

A
|
A

I

S

M
|
M

E

E

V

I

Y

H

D

Q

A

A

L

L

F

T

-

R

D

S

A

S

P

S

L

I

R

R

N

N

I

V

L

L

A

P

R

R

H

H

E
|
E

Q

Q

A

G

A

V

H

F

M

A

A

A

D

E

G

G

Y

Y

A

A

R

R

A

S

T

S

G

G

K

K

V

P

G

G

V

I

V

C

L

I

A

A

T

T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

G

G

I

L

A

A

T

D

A

A

Y

L

M

L

D

D

S
|
S

V

V

P

P

L

L

V

V

A

A

I

I

T

T

G

G

Q

Q

V

V

P
|
P

T

R

S
x
R

Y

M

I

I

G

G

T

T

D

D

A

A

F

F

Q
|
Q

E

E

A

T

D

P

V

I

V

V

G

E

I

V

T

T

R

R

P

S

I

I

T

T

K
|
K

H

H

N

N

F

Y

L

L

V

V

T

M

D

D

I

V

K

E

D

D

L

I

A

P

L

R

I

I

L

I

R

E

E

E

A

A

F

F

Y

F

L

L

A

A

R

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

V

V

D

D

I

V

P

P

K
|
K

D

D

I

I

T

Q

Q

Q

E

-

Y

-

D

-

Y

L

K

A

M

I

P

P

T

N

E

W

K

E

D

Q

V

A

L

M

D

-

A

R

L

L

P

P

G

G

Y

Y

K

M

P

S

H

R

Y

M

E

P

G

K

N

P

P

P

-

E

-

D

F

S

Q

H

I

L

K

E

K

Q

A

I

A

V

E

R

L

L

I

I

R

S

K

E

A

S

K

K

R

K

P

P

V

V

L

L

Y

Y

V

V

G

G

G
|
G

G

G

T

C

V

L

I

N

S

S

G

-

A

-

S

S

E

D

E

E

L

L

R

G

E

R

L

F

A

V

E

E

L

L

T

T

R

G

I

I

P

P

V

V

T

A

T

S

T
|
T

N
x
L

M

M

A

G

K

L

G

G

A

S

F

Y

D

P

E
x
C

T

D

H

D

P

E

L

L

A

S

L

L

H

H

M

M

L
|
L

G
|
G

M
|
M

H
|
H

G

G

T

T

Y

V

Y

Y

A

A

N

N

M

Y

A

A

V

V

Y

E

H

H

S

S

D

D

L

L

I

L

A

A

V

F

G
|
G

A
x
V

R
|
R

F
|
F

D
|
D

R
|
R

V

V

T

T

G

G

K

K

I

L

Q

E

E

A

F

F

A

A

P

S

E

R

A

A

K

K

I

I

I

V

H

H

I

I

D
|
D

I
|
I

D

D

P

S

A

A

S

E

I

I

S

G

K

K

N

N

I

K

T

T

V

P

D

H

V

V

P

S

I

V

V

C

G

G

D
|
D

V
|
V

K

K

V

L

V

A

L

L

G

Q

K

G

L

M

L

N

K

K

E

V

L

L

K

E

K

N

K

R

P

A

I

E

E

E

W

L

V

K

K

L

A

D

R

F

E

G

S

V

W

W

L

R

K

N

Q

E

I

L

E

N

K

V

W

Q

K

K

E

Q

K

K

H

F

P

P

L

L

T

S

Y

F

D

K

R

T

T

F

S

G

K

E

I

A

I

I

K

P

P

P

Q

Q

Y

Y

V

A

I

I

E

K

E

V

I

L

Y

D

K

E

I

L

T

T

D

D

G

G

E

K

A

A

L

I

I

I

T

S

A

T

G

G

V

V

G

G

Q
|
Q

H
|
H

Q

Q

M

M

W

W

A

A

M

Q

F

F

Y

Y

K

N

Y

Y

R

K

Y

K

P

P

R

R

Q

Q

F

W

L

L

N

S

S

S

G
|
G

L

L

G
|
G

T
x
A

M
|
M

G

G

Y

F

G

G

F

L

P

P

A

A

V

I

G

G

A

A

K

S

I

V

G

A

N

N

P

P

D

D

K

A

T

I

V

V

F

V

A

D

I

I

E

D

G

G

D
|
D

G
|
G

S
|
S

F

F

V

I

M

M

N

N

M

V

Q

Q

D

E

V

L

I

A

T

T

A

I

V

R

Q

V

Y

E

R

N

I

L

P

P

V

V

K

K

I

V

A

L

I

L

N

N

N
|
N

E
x
Q

F
x
H

L
|
L

G
|
G

M
|
M

V

V

R

M

Q

Q

W
|
W

Q

E

Q

D

L

R

F

F

Y

Y

D

K

S

A

R

N

Y

R

S

A

S

H

V

T

C

F

L

L

A

G

-

D

-

P

-

A

-

Q

-

E

-

D

-

E

V

I

H

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

S

A

M

C

G

G

A

I

V

P

G

A

L

A

R

R

A

V

T

T

K

K

P

K

E

A

E

D

V

L

R

R

P

E

V

A

L

I

E

Q

K

-

A

T

M

M

K

L

I

D

N

T

D

P

R

G

P

P

V

Y

I

L

M

L

D

D

F

V

V

I

V

C

D

P

R

H

E

Q

E

E

N

H

V

V

L

L

P

P

M

M

V

I

P

P

A
x
S

G

G

K

G

S

T

Y

F

R

N

E

D

M

V

I

I

A122 (= A29) mutation to V: Reduced catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
M124 (= M31) mutation to E: Reduced catalytic activity. Resistant to imidazolinone herbicides and reduced sensitivity to sulfonylurea herbicides.; mutation to I: No effect on catalytic activity. Increased resistance to imidazolinone herbicides.
E144 (= E50) binding thiamine diphosphate
S186 (= S92) binding FAD
P197 (= P103) mutation to S: In csr1-1/GH50; resistant to sulfonylurea but not to imidazolinone herbicides.
R199 (≠ S105) mutation R->A,E: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
Q207 (= Q113) binding thiamine diphosphate
K220 (= K126) binding (R)-imazaquin
R246 (= R152) binding (R)-imazaquin; binding FAD
K256 (= K162) binding chlorimuron-ethyl
G308 (= G216) binding FAD
TL 331:332 (≠ TN 241:242) binding FAD
C340 (≠ E250) modified: Cysteine sulfinic acid (-SO2H)
LGMH 349:352 (= LGMH 259:262) binding FAD
GVRFD 371:375 (≠ GARFD 281:285) binding FAD
DR 376:377 (= DR 286:287) binding chlorimuron-ethyl
DI 395:396 (= DI 305:306) binding FAD
DV 414:415 (= DV 324:325) binding FAD
QH 487:488 (= QH 397:398) binding thiamine diphosphate
GG 508:509 (= GG 418:419) binding FAD
GAM 511:513 (≠ GTM 421:423) binding thiamine diphosphate
D538 (= D448) binding Mg(2+)
DGS 538:540 (= DGS 448:450) binding thiamine diphosphate
N565 (= N475) binding Mg(2+)
NQHLGM 565:570 (≠ NEFLGM 475:480) binding thiamine diphosphate
H567 (≠ F477) binding Mg(2+)
W574 (= W484) binding chlorimuron-ethyl; mutation to L: Increased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.; mutation to S: Slightly decreased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.
S653 (≠ A557) binding chlorimuron-ethyl; mutation to A: No effect on catalytic activity or sensitivity to herbicides.; mutation to F: No effect on catalytic activity. Resistant to imidazolinone herbicides and also slightly sulfonylurea-resistant.; mutation to N: In csr1-2/GH90; no effect on catalytic activity. Resistant to imidazolinone but not to sulfonylurea herbicides.; mutation to T: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.

5k3sA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, bispyribac-sodium (see paper)
48% identity, 97% coverage: 4:565/581 of query aligns to 12:576/583 of 5k3sA

query
sites
5k3sA

K

R

R

K

G

G

A

A

D

D

I

I

V

L

I

V

D

E

V

A

L

L

L

E

E

R

E

Q

G

G

V

V

D

E

T

T

V

V

F

F

G

A

L
x
Y

P

P

G
|
G

A
|
A

I
x
S

M

M

E

E

V

I

Y

H

D

Q

A

A

L

L

F

T

-

R

D

S

A

S

P

S

L

I

R

R

N

N

I

V

L

L

A

P

R

R

H

H

E
|
E

Q

Q

A

G

A

V

H

F

M

A

A

A

D

E

G

G

Y

Y

A

A

R

R

A

S

T

S

G

G

K

K

V

P

G

G

V

I

V

C

L

I

A

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

G

G

I

L

A

A

T

D

A

A

Y

L

M

L

D

D

S

S

V

V

P

P

L

L

V

V

A

A

I

I

T

T

G

G

Q

Q

V

V

P

P

T

R

S

R

Y

M

I

I

G

G

T

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

A

T

D

P

V

I

V

V

G

E

I

V

T

T

R

R

P

S

I

I

T

T

K

K

H

H

N

N

F

Y

L

L

V

V

T

M

D

D

I

V

K

E

D

D

L

I

A

P

L

R

I

I

L

I

R

E

E

E

A

A

F

F

Y

F

L

L

A

A

R

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

V

V

D

D

I

V

P

P

K
|
K

D

D

I

I

T

Q

Q

Q

E

-

Y

-

D

-

Y

L

K

A

M

I

P

P

T

N

E

W

K

E

D

Q

V

A

L

M

D

-

A

R

L

L

P

P

G

G

Y

Y

K

M

P

S

H

R

Y

M

E

P

G

K

N

P

P

P

-

E

-

D

F

S

Q

H

I

L

K

E

K

Q

A

I

A

V

E

R

L

L

I

I

R

S

K

E

A

S

K

K

R

K

P

P

V

V

L

L

Y

Y

V

V

G
|
G

T

C

V

L

I

N

S

S

G

-

A

-

S

S

E

D

E

E

L

L

R

G

E

R

L

F

A

V

E

E

L

L

T

T

R

G

I

I

P

P

V

V

T

A

T

S

T
|
T

N
x
L

M
|
M

A

G

K

L

G

G

A

S

F

Y

D

P

E

C

T

D

H

D

P

E

L

L

A

S

L

L

H

H

M

M

L
|
L

G

G

M
|
M

H

H

G

G

T

T

Y

V

Y

Y

A

A

N

N

M

Y

A

A

V

V

Y

E

H

H

S

S

D

D

L

L

I

L

A

A

V

F

G
|
G

A

V

R
|
R

F

F

D
|
D

D

D

R
|
R

V
|
V

T

T

G

G

K

K

I

L

Q

E

E

A

F

F

A

A

P

S

E

R

A

A

K

K

I

I

I

V

H

H

I

I

D
|
D

I
|
I

D

D

P

S

A

A

S

E

I

I

S

G

K

K

N

N

I

K

T

T

V

P

D

H

V

V

P

S

I

V

V

C

G

G

D
|
D

V
|
V

K

K

V

L

V

A

L

L

G

Q

K

G

L

M

L

N

K

K

E

V

L

L

K

E

K

N

K

R

P

A

I

E

E

E

W

L

V

K

K

L

A

D

R

F

E

G

S

V

W

W

L

R

K

N

Q

E

I

L

E

N

K

V

W

Q

K

K

E

Q

K

K

H

F

P

P

L

L

T

S

Y

F

D

K

R

T

T

F

S

G

K

E

I

A

I

I

K

P

P

P

Q

Q

Y

Y

V

A

I

I

E

K

E

V

I

L

Y

D

K

E

I

L

T

T

D

D

G

G

E

K

A

A

L

I

I

I

T

S

A

T

G

G

V
|
V

G
|
G

Q
|
Q

H
|
H

Q

Q

M
|
M

W

W

A

A

M

Q

F

F

Y

Y

K

N

Y

Y

R

K

Y

K

P

P

R

R

Q

Q

F

W

L

L

N

S

S

S

G
|
G

G

G

L

L

G
|
G

T

A

M
|
M

G

G

Y

F

G

G

F

L

P

P

A

A

V

I

G

G

A

A

K

S

I

V

G

A

N

N

P

P

D

D

K

A

T

I

V

V

F

V

A

D

I

I

E

D

G

G

D
|
D

G
|
G

S
|
S

F

F

V

I

M

M

N

N

M

V

Q

Q

D

E

V

L

I

A

T

T

A

I

V

R

Q

V

Y

E

R

N

I

L

P

P

V

V

K

K

I

V

A

L

I

L

N

N

N
|
N

E

Q

F
x
H

L
|
L

G
|
G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

Q

D

L

R

F

F

Y

Y

D

K

S

A

R

N

Y

R

S

A

S

H

V

T

C

F

L

L

A

G

-

D

-

P

-

A

-

Q

-

E

-

D

-

E

V

I

H

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

S

A

M

C

G

G

A

I

V

P

G

A

L

A

R

R

A

V

T

T

K

K

P

K

E

A

E

D

V

L

R

R

P

E

V

A

L

I

E

Q

K

-

A

T

M

M

K

L

I

D

N

T

D

P

R

G

P

P

V

Y

I

L

M

L

D

D

F

V

V

I

V

C

D

P

R
x
H

E

Q

E

E

N

H

V

V

L

L

P

P

M

M

V

I

P

P

A

S

G
|
G

K

G

S

T

Y

F

R

N

E

D

M

V

I

I

active site: Y33 (≠ L25), G35 (= G27), G36 (= G28), A37 (= A29), S38 (≠ I30), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), M266 (= M261), V293 (= V288), V400 (= V395), G426 (= G421), M428 (= M423), D453 (= D448), N480 (= N475), H482 (≠ F477), L483 (= L478), M485 (= M480), V486 (= V481), W489 (= W484), H558 (≠ R547)
binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: R292 (= R287), M485 (= M480), W489 (= W484), G569 (= G558)
binding flavin-adenine dinucleotide: R161 (= R152), G222 (= G215), G223 (= G216), G224 (= G217), T246 (= T241), L247 (≠ N242), M248 (= M243), L264 (= L259), M266 (= M261), G286 (= G281), R288 (= R283), D290 (= D285), V293 (= V288), D310 (= D305), I311 (= I306), D329 (= D324), V330 (= V325), M405 (= M400), G423 (= G418)
binding magnesium ion: D453 (= D448), N480 (= N475), H482 (≠ F477)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V395), G401 (= G396), Q402 (= Q397), H403 (= H398), G426 (= G421), M428 (= M423), D453 (= D448), G454 (= G449), S455 (= S450), N480 (= N475), H482 (≠ F477), L483 (= L478), G484 (= G479), M485 (= M480), V486 (= V481)

P09114 Acetolactate synthase 2, chloroplastic; ALS II; Acetohydroxy-acid synthase II; Acetolactate synthase II; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see paper)
48% identity, 99% coverage: 4:578/581 of query aligns to 91:664/664 of P09114

query
sites
P09114

K

R

R

K

G

G

A

S

D

D

I

V

V

L

I

V

D

E

V

A

L

L

L

E

E

R

E

E

G

G

V

V

D

T

T

D

V

V

F

F

G

A

L

Y

P

P

G

G

A

A

I

S

M

M

E

E

V

I

Y

H

D

Q

A

A

L

L

F

T

D

R

A

S

P

S

L

I

-

I

R

R

N

N

I

V

L

L

A

P

R

R

H

H

E

E

Q

Q

A

G

A

V

H

F

M

A

A

A

D

E

G

G

Y

Y

A

A

R

R

A

A

T

T

G

G

K

F

V

P

G

G

V

V

V

C

L

I

A

A

T

T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

G

G

I

L

A

A

T

D

A

A

Y

L

M

L

D

D

S

S

V

V

P

P

L

I

V

V

A

A

I

I

T

T

G

G

Q

Q

V

V

P
|
P

T

R

S

R

Y

M

I

I

G

G

T

T

D

D

A

A

F

F

Q

Q

E

E

A

T

D

P

V

I

V

V

G

E

I

V

T

T

R

R

P

S

I

I

T

T

K

K

H

H

N

N

F

Y

L

L

V

V

T

M

D

D

I

V

K

E

D

D

L

I

A

P

L

R

I

V

L

V

R

R

E

E

A

A

F

F

Y

F

L

L

A

A

R

R

T

S

G

G

R

R

P

P

G

G

P

P

V

V

L

L

V

I

D

D

I

V

P

P

K

K

D

D

I

I

T

Q

Q

Q

E

L

Y

V

-

I

-

P

D

D

Y

W

K

D

M

Q

P

P

T

M

E

R

K

-

D

-

V

-

L

-

D

-

A

-

L

L

P

P

G

G

Y

Y

K

M

P

S

H

R

Y

L

E

P

G

K

N

L

P

P

F

N

Q

E

-

M

-

L

I

L

K

E

K

Q

A

I

A

V

E

R

L

L

I

I

R

S

K

E

A

S

K

K

R

K

P

P

V

V

L

L

Y

Y

V

V

G

G

T

C

V

-

I

-

S

S

G

Q

A

S

S

S

E

E

L

L

R

R

E

R

L

F

A

V

E

E

L

L

T

T

R

G

I

I

P

P

V

V

T

A

T

S

T

T

N

L

M

M

A

G

K

L

G

G

A

A

F

F

D

P

E

T

T

G

H

D

P

E

L

L

A

S

L

L

H

S

M

M

L

L

G

G

M

M

H

H

G

G

T

T

Y

V

Y

Y

A

A

N

N

M

Y

A

A

V

V

Y

D

H

S

S

S

D

D

L

L

I

L

A

A

V

F

G

G

A

V

R

R

F

F

D

D

R

R

V

V

T

T

G

G

K

K

I

L

Q

E

E

A

F

F

A

A

P

S

E

R

A

A

K

K

I

I

I

V

H

H

I

I

D

D

I

I

D

D

P

S

A

A

S

E

I

I

S

G

K

K

N

N

I

K

T

Q

V

P

D

H

V

V

P

S

I

I

V

C

G

A

D

D

V

I

K

K

V

L

V

A

L

L

G

Q

K

G

L

L

L

N

K

S

E

I

L

L

K

E

K

S

K

K

P

E

I

G

E

K

W

L

V

K

K

L

A

D

R

F

E

S

S

A

W

W

L

R

K

Q

Q

E

I

L

E

T

K

V

W

Q

K

K

E

V

K

K

H

Y

P

P

L

L

T

N

Y

F

D

K

R

T

T

F

S

G

K

D

I

A

I

I

K

P

P

P

Q

Q

Y

Y

V

A

I

I

E

Q

E

V

I

L

Y

D

K

E

I

L

T

T

D

N

G

G

E

S

A

A

L

I

I

I

T

S

A

T

G

G

V

V

G

G

Q

Q

H

H

Q

Q

M

M

W

W

A

A

M

Q

F

Y

Y

Y

K

K

Y

Y

R

R

Y

K

P

P

R

R

Q

Q

F

W

L

L

N

T

S

S

G

G

L

L

G

G

T

A

M

M

G

G

Y

F

G

G

F

L

P

P

A

A

V

I

G

G

A

A

K

A

I

V

G

G

N

R

P

P

D

D

K

E

T

V

V

V

F

V

A

D

I

I

E

D

G

G

D

D

G

G

S

S

F

F

V

I

M

M

N

N

M

V

Q

Q

D

E

V

L

I

A

T

T

A

I

V

K

Q

V

Y

E

R

N

I

L

P

P

V

V

K

K

I

I

A

M

I

L

N

N

E

Q

F

H

L

L

G

G

M

M

V

V

R

V

Q

Q

W
|
W

Q

E

Q

D

L

R

F

F

Y

Y

D

K

S

A

R

N

Y

R

S

A

S

H

V

T

C

Y

L

L

A

G

-

N

-

P

-

S

-

N

-

E

-

A

-

E

V

I

H

F

P

P

D

N

F

M

V

L

K

K

L

F

A

A

E

E

S

A

M

C

G

G

A

V

V

P

G

A

L

A

R

R

A

V

T

T

K

H

P

R

E

D

V

L

R

R

P

A

V

A

L

I

E

Q

K

K

A

M

M

L

K

D

I

-

N

T

D

P

R

G

P

P

V

Y

I

L

M

L

D

D

F

V

V

I

V

V

D

P

R

H

E

Q

E

E

N

H

V

V

L

L

P

P

M

M

V

I

P

P

A

S

G

G

K

G

S

A

Y

F

R

K

E

D

M

V

I

I

V

T

S

-

P

-

K

-

Q

-

K

E

G

G

E

D

A

G

E

R

T

S

M

S

Y

Y

P191 (= P103) mutation to A: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with L-568.
W568 (= W484) mutation to L: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with A-191.

5k2oA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, pyrithiobac (see paper)
48% identity, 97% coverage: 4:565/581 of query aligns to 12:576/585 of 5k2oA

query
sites
5k2oA

K

R

R

K

G

G

A

A

D

D

I

I

V

L

I

V

D

E

V

A

L

L

L

E

E

R

E

Q

G

G

V

V

D

E

T

T

V

V

F

F

G

A

L
x
Y

P

P

G
|
G

A
|
A

I
x
S

M

M

E

E

V

I

Y

H

D

Q

A

A

L

L

F

T

-

R

D

S

A

S

P

S

L

I

R

R

N

N

I

V

L

L

A

P

R

R

H

H

E
|
E

Q

Q

A

G

A

V

H

F

M

A

A

A

D

E

G

G

Y

Y

A

A

R

R

A

S

T

S

G

G

K

K

V

P

G

G

V

I

V

C

L

I

A

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

G

G

I

L

A

A

T

D

A

A

Y

L

M

L

D

D

S

S

V

V

P

P

L

L

V

V

A

A

I

I

T

T

G

G

Q

Q

V

V

P

P

T

R

S

R

Y

M

I

I

G

G

T

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

A

T

D

P

V

I

V

V

G

E

I

V

T

T

R

R

P

S

I

I

T

T

K

K

H

H

N

N

F

Y

L

L

V

V

T

M

D

D

I

V

K

E

D

D

L

I

A

P

L

R

I

I

L

I

R

E

E

E

A

A

F

F

Y

F

L

L

A

A

R

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

V

V

D

D

I

V

P

P

K
|
K

D

D

I

I

T

Q

Q

Q

E

-

Y

-

D

-

Y

L

K

A

M

I

P

P

T

N

E

W

K

E

D

Q

V

A

L

M

D

-

A

R

L

L

P

P

G

G

Y

Y

K

M

P

S

H

R

Y

M

E

P

G

K

N

P

P

P

-

E

-

D

F

S

Q

H

I

L

K

E

K

Q

A

I

A

V

E

R

L

L

I

I

R

S

K

E

A

S

K

K

R

K

P

P

V

V

L

L

Y

Y

V

V

G
|
G

T

C

V

L

I

N

S

S

G

-

A

-

S

S

E

D

E

E

L

L

R

G

E

R

L

F

A

V

E

E

L

L

T

T

R

G

I

I

P

P

V

V

T

A

T

S

T
|
T

N
x
L

M
|
M

A

G

K

L

G

G

A

S

F

Y

D

P

E

C

T

D

H

D

P

E

L

L

A

S

L

L

H

H

M

M

L
|
L

G

G

M
|
M

H

H

G

G

T

T

Y

V

Y

Y

A

A

N

N

M

Y

A

A

V

V

Y

E

H

H

S

S

D

D

L

L

I

L

A

A

V

F

G
|
G

A

V

R
|
R

F

F

D
|
D

D

D

R
|
R

V
|
V

T

T

G

G

K

K

I

L

Q

E

E

A

F

F

A

A

P

S

E

R

A

A

K

K

I

I

I

V

H

H

I

I

D
|
D

I
|
I

D

D

P

S

A

A

S

E

I

I

S

G

K

K

N

N

I

K

T

T

V

P

D

H

V

V

P

S

I

V

V

C

G

G

D
|
D

V
|
V

K

K

V

L

V

A

L

L

G

Q

K

G

L

M

L

N

K

K

E

V

L

L

K

E

K

N

K

R

P

A

I

E

E

E

W

L

V

K

K

L

A

D

R

F

E

G

S

V

W

W

L

R

K

N

Q

E

I

L

E

N

K

V

W

Q

K

K

E

Q

K

K

H

F

P

P

L

L

T

S

Y

F

D

K

R

T

T

F

S

G

K

E

I

A

I

I

K

P

P

P

Q

Q

Y

Y

V

A

I

I

E

K

E

V

I

L

Y

D

K

E

I

L

T

T

D

D

G

G

E

K

A

A

L

I

I

I

T

S

A

T

G

G

V
|
V

G
|
G

Q
|
Q

H
|
H

Q
|
Q

M
|
M

W

W

A

A

M

Q

F

F

Y

Y

K

N

Y

Y

R

K

Y

K

P

P

R

R

Q

Q

F

W

L

L

N

S

S

S

G
|
G

G

G

L

L

G
|
G

T

A

M
|
M

G

G

Y

F

G

G

F

L

P

P

A

A

V

I

G

G

A

A

K

S

I

V

G

A

N

N

P

P

D

D

K

A

T

I

V

V

F

V

A

D

I

I

E

D

G

G

D
|
D

G
|
G

S
|
S

F

F

V

I

M

M

N

N

M

V

Q

Q

D

E

V

L

I

A

T

T

A

I

V

R

Q

V

Y

E

R

N

I

L

P

P

V

V

K

K

I

V

A

L

I

L

N

N

N
|
N

E

Q

F
x
H

L
|
L

G
|
G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

Q

D

L

R

F

F

Y

Y

D

K

S

A

R

N

Y

R

S

A

S

H

V

T

C

F

L

L

A

G

-

D

-

P

-

A

-

Q

-

E

-

D

-

E

V

I

H

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

S

A

M

C

G

G

A

I

V

P

G

A

L

A

R

R

A

V

T

T

K

K

P

K

E

A

E

D

V

L

R

R

P

E

V

A

L

I

E

Q

K

-

A

T

M

M

K

L

I

D

N

T

D

P

R

G

P

P

V

Y

I

L

M

L

D

D

F

V

V

I

V

C

D

P

R
x
H

E

Q

E

E

N

H

V

V

L

L

P

P

M

M

V

I

P

P

A
x
S

G

G

K

G

S

T

Y

F

R

N

E

D

M

V

I

I

active site: Y33 (≠ L25), G35 (= G27), G36 (= G28), A37 (= A29), S38 (≠ I30), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), M266 (= M261), V293 (= V288), V400 (= V395), G426 (= G421), M428 (= M423), D453 (= D448), N480 (= N475), H482 (≠ F477), L483 (= L478), M485 (= M480), V486 (= V481), W489 (= W484), H558 (≠ R547)
binding 2-chloranyl-6-(4,6-dimethoxypyrimidin-2-yl)sulfanyl-benzoic acid: M266 (= M261), R292 (= R287), W489 (= W484), S568 (≠ A557)
binding flavin-adenine dinucleotide: R161 (= R152), G222 (= G215), G223 (= G216), G224 (= G217), T246 (= T241), L247 (≠ N242), M248 (= M243), L264 (= L259), G286 (= G281), R288 (= R283), D290 (= D285), V293 (= V288), D310 (= D305), I311 (= I306), D329 (= D324), V330 (= V325), Q404 (= Q399), M405 (= M400), G423 (= G418)
binding magnesium ion: D453 (= D448), N480 (= N475), H482 (≠ F477)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V395), G401 (= G396), Q402 (= Q397), H403 (= H398), M428 (= M423), D453 (= D448), G454 (= G449), S455 (= S450), N480 (= N475), H482 (≠ F477), L483 (= L478), G484 (= G479), M485 (= M480), V486 (= V481)

8et4A Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with amidosulfuron (see paper)
48% identity, 97% coverage: 4:565/581 of query aligns to 12:576/582 of 8et4A

query
sites
8et4A

K

R

R

K

G

G

A

A

D

D

I

I

V

L

I

V

D

E

V

A

L

L

L

E

E

R

E

Q

G

G

V

V

D

E

T

T

V

V

F

F

G

A

L

Y

P

P

G

G

A

A

I

S

M

M

E

E

V

I

Y

H

D

Q

A

A

L

L

F

T

-

R

D

S

A

S

P

S

L

I

R

R

N

N

I

V

L

L

A

P

R

R

H

H

E

E

Q

Q

A

G

A

V

H

F

M

A

A

A

D

E

G

G

Y

Y

A

A

R

R

A

S

T

S

G

G

K

K

V

P

G

G

V

I

V

C

L

I

A

A

T

T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

G

G

I

L

A

A

T

D

A

A

Y

L

M

L

D

D

S

S

V

V

P

P

L

L

V

V

A

A

I

I

T

T

G

G

Q

Q

V

V

P

P

T

R

S

R

Y

M

I

I

G

G

T

T

D

D

A

A

F

F

Q

Q

E

E

A

T

D

P

V

I

V

V

G

E

I

V

T

T

R

R

P

S

I

I

T

T

K

K

H

H

N

N

F

Y

L

L

V

V

T

M

D

D

I

V

K

E

D

D

L

I

A

P

L

R

I

I

L

I

R

E

E

E

A

A

F

F

Y

F

L

L

A

A

R

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

V

V

D

D

I

V

P

P

K

K

D

D

I

I

T

Q

Q

Q

E

-

Y

-

D

-

Y

L

K

A

M

I

P

P

T

N

E

W

K

E

D

Q

V

A

L

M

D

-

A

R

L

L

P

P

G

G

Y

Y

K

M

P

S

H

R

Y

M

E

P

G

K

N

P

P

P

-

E

-

D

F

S

Q

H

I

L

K

E

K

Q

A

I

A

V

E

R

L

L

I

I

R

S

K

E

A

S

K

K

R

K

P

P

V

V

L

L

Y

Y

V

V

G
|
G

T

C

V

L

I

N

S

S

G

-

A

-

S

S

E

D

E

E

L

L

R

G

E

R

L

F

A

V

E

E

L

L

T

T

R

G

I

I

P

P

V

V

T

A

T

S

T
|
T

N
x
L

M
|
M

A

G

K

L

G

G

A

S

F

Y

D

P

E

C

T

D

H

D

P

E

L

L

A

S

L

L

H

H

M

M

L
|
L

G

G

M
|
M

H
|
H

G

G

T

T

Y

V

Y

Y

A

A

N

N

M

Y

A

A

V

V

Y

E

H

H

S

S

D

D

L

L

I

L

A

A

V

F

G
|
G

A
x
V

R
|
R

F

F

D
|
D

D

D

R
|
R

V
|
V

T

T

G

G

K

K

I

L

Q

E

E

A

F

F

A

A

P

S

E

R

A

A

K

K

I

I

I

V

H

H

I

I

D
|
D

I
|
I

D

D

P

S

A

A

S

E

I

I

S

G

K

K

N

N

I

K

T

T

V

P

D

H

V

V

P

S

I

V

V

C

G

G

D
|
D

V
|
V

K

K

V

L

V

A

L

L

G

Q

K

G

L

M

L

N

K

K

E

V

L

L

K

E

K

N

K

R

P

A

I

E

E

E

W

L

V

K

K

L

A

D

R

F

E

G

S

V

W

W

L

R

K

N

Q

E

I

L

E

N

K

V

W

Q

K

K

E

Q

K

K

H

F

P

P

L

L

T

S

Y
x
F

D

K

R

T

T

F

S

G

K

E

I

A

I

I

K

P

P

P

Q

Q

Y

Y

V

A

I

I

E

K

E

V

I

L

Y

D

K

E

I

L

T

T

D

D

G

G

E

K

A

A

L

I

I

I

T

S

A

T

G

G

V
|
V

G
|
G

Q
|
Q

H
|
H

Q

Q

M
|
M

W

W

A

A

M

Q

F

F

Y

Y

K

N

Y

Y

R

K

Y

K

P

P

R

R

Q

Q

F

W

L

L

N

S

S

S

G
|
G

G

G

L

L

G
|
G

T

A

M
|
M

G

G

Y

F

G

G

F

L

P

P

A

A

V

I

G

G

A

A

K

S

I

V

G

A

N

N

P

P

D

D

K

A

T

I

V

V

F

V

A

D

I

I

E

D

G
|
G

D
|
D

G
|
G

S
|
S

F

F

V

I

M
|
M

N

N

M

V

Q
|
Q

D

E

V

L

I

A

T

T

A

I

V

R

Q

V

Y

E

R

N

I

L

P

P

V

V

K

K

I

V

A

L

I

L

N

N

N
|
N

E

Q

F
x
H

L
|
L

G
|
G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

Q

D

L

R

F

F

Y

Y

D

K

S

A

R

N

Y

R

S

A

S

H

V

T

C

F

L

L

A

G

-

D

-

P

-

A

-

Q

-

E

-

D

-

E

V

I

H

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

S

A

M

C

G

G

A

I

V

P

G

A

L

A

R

R

A

V

T

T

K

K

P
x
K

E

A

E

D

V

L

R

R

P

E

V

A

L

I

E

Q

K

-

A

T

M

M

K

L

I

D

N

T

D

P

R

G

P

P

V

Y

I

L

M

L

D

D

F

V

V

I

V

C

D

P

R

H

E

Q

E

E

N

H

V

V

L

L

P

P

M

M

V

I

P

P

A
x
S

G

G

K

G

S

T

Y

F

R

N

E

D

M

V

I

I

binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V395), G401 (= G396), Q402 (= Q397), H403 (= H398), G426 (= G421), M428 (= M423), G452 (= G447), D453 (= D448), G454 (= G449), S455 (= S450), M458 (= M453), N480 (= N475), H482 (≠ F477), L483 (= L478), G484 (= G479), M485 (= M480), V486 (= V481)
binding flavin-adenine dinucleotide: R161 (= R152), G222 (= G215), G223 (= G216), G224 (= G217), T246 (= T241), L247 (≠ N242), M248 (= M243), L264 (= L259), M266 (= M261), H267 (= H262), G286 (= G281), V287 (≠ A282), R288 (= R283), D290 (= D285), R292 (= R287), V293 (= V288), D310 (= D305), I311 (= I306), D329 (= D324), V330 (= V325), M405 (= M400), G423 (= G418)
binding magnesium ion: F370 (≠ Y365), D453 (= D448), M458 (= M453), Q461 (= Q456), N480 (= N475), H482 (≠ F477), K533 (≠ P521)
binding N-{[(4,6-dimethoxypyrimidin-2-yl)carbamoyl]sulfamoyl}-N-methylmethanesulfonamide: M266 (= M261), R292 (= R287), M485 (= M480), W489 (= W484), S568 (≠ A557)

5wj1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a triazolopyrimidine herbicide, penoxsulam (see paper)
48% identity, 97% coverage: 4:565/581 of query aligns to 12:576/582 of 5wj1A

query
sites
5wj1A

K

R

R

K

G

G

A

A

D

D

I

I

V

L

I

V

D

E

V

A

L

L

L

E

E

R

E

Q

G

G

V

V

D

E

T

T

V

V

F

F

G

A

L
x
Y

P

P

G
|
G

A
|
A

I
x
S

M

M

E

E

V

I

Y

H

D

Q

A

A

L

L

F

T

-

R

D

S

A

S

P

S

L

I

R

R

N

N

I

V

L

L

A

P

R

R

H

H

E
|
E

Q

Q

A

G

A

V

H

F

M

A

A

A

D

E

G

G

Y

Y

A

A

R

R

A

S

T

S

G

G

K

K

V

P

G

G

V

I

V

C

L

I

A

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

G

G

I

L

A

A

T

D

A

A

Y

L

M

L

D

D

S

S

V

V

P

P

L

L

V

V

A

A

I

I

T

T

G

G

Q

Q

V

V

P

P

T

R

S

R

Y

M

I

I

G

G

T

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

A

T

D

P

V

I

V

V

G

E

I

V

T

T

R

R

P

S

I

I

T

T

K

K

H

H

N

N

F

Y

L

L

V

V

T

M

D

D

I

V

K

E

D

D

L

I

A

P

L

R

I

I

L

I

R

E

E

E

A

A

F

F

Y

F

L

L

A

A

R

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

V

V

D

D

I

V

P

P

K
|
K

D

D

I

I

T

Q

Q

Q

E

-

Y

-

D

-

Y

L

K

A

M

I

P

P

T

N

E

W

K

E

D

Q

V

A

L

M

D

-

A

R

L

L

P

P

G

G

Y

Y

K

M

P

S

H

R

Y

M

E

P

G

K

N

P

P

P

-

E

-

D

F

S

Q

H

I

L

K

E

K

Q

A

I

A

V

E

R

L

L

I

I

R

S

K

E

A

S

K

K

R

K

P

P

V

V

L

L

Y

Y

V

V

G
|
G

T

C

V

L

I

N

S

S

G

-

A

-

S

S

E

D

E

E

L

L

R

G

E

R

L

F

A

V

E

E

L

L

T

T

R

G

I

I

P

P

V

V

T

A

T

S

T
|
T

N
x
L

M
|
M

A

G

K

L

G

G

A

S

F

Y

D

P

E

C

T

D

H

D

P

E

L

L

A

S

L

L

H

H

M
|
M

L
|
L

G

G

M
|
M

H

H

G

G

T

T

Y

V

Y

Y

A

A

N

N

M

Y

A

A

V

V

Y

E

H

H

S

S

D

D

L

L

I

L

A

A

V

F

G
|
G

A

V

R
|
R

F

F

D

D

D
|
D

R
|
R

V
|
V

T

T

G

G

K

K

I

L

Q

E

E

A

F

F

A

A

P

S

E

R

A

A

K

K

I

I

I

V

H

H

I

I

D
|
D

I
|
I

D

D

P

S

A

A

S

E

I

I

S

G

K

K

N

N

I

K

T

T

V

P

D

H

V

V

P

S

I

V

V

C

G

G

D
|
D

V
|
V

K

K

V

L

V

A

L

L

G

Q

K

G

L

M

L

N

K

K

E

V

L

L

K

E

K

N

K

R

P

A

I

E

E

E

W

L

V

K

K

L

A

D

R

F

E

G

S

V

W

W

L

R

K

N

Q

E

I

L

E

N

K

V

W

Q

K

K

E

Q

K

K

H

F

P

P

L

L

T

S

Y

F

D

K

R

T

T

F

S

G

K

E

I

A

I

I

K

P

P

P

Q

Q

Y

Y

V

A

I

I

E

K

E

V

I

L

Y

D

K

E

I

L

T

T

D

D

G

G

E

K

A

A

L

I

I

I

T

S

A

T

G

G

V
|
V

G
|
G

Q
|
Q

H
|
H

Q

Q

M
|
M

W

W

A

A

M

Q

F

F

Y

Y

K

N

Y

Y

R

K

Y

K

P

P

R

R

Q

Q

F

W

L

L

N

S

S

S

G
|
G

L

L

G
|
G

T

A

M
|
M

G

G

Y

F

G

G

F

L

P

P

A

A

V

I

G

G

A

A

K

S

I

V

G

A

N

N

P

P

D

D

K

A

T

I

V

V

F

V

A

D

I

I

E

D

G

G

D
|
D

G
|
G

S
|
S

F

F

V

I

M
|
M

N

N

M

V

Q

Q

D

E

V

L

I

A

T

T

A

I

V

R

Q

V

Y

E

R

N

I

L

P

P

V

V

K

K

I

V

A

L

I

L

N

N

N
|
N

E

Q

F
x
H

L
|
L

G
|
G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

Q

D

L

R

F

F

Y

Y

D

K

S

A

R

N

Y

R

S

A

S

H

V

T

C

F

L

L

A

G

-

D

-

P

-

A

-

Q

-

E

-

D

-

E

V

I

H

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

S

A

M

C

G

G

A

I

V

P

G

A

L

A

R

R

A

V

T

T

K

K

P

K

E

A

E

D

V

L

R

R

P

E

V

A

L

I

E

Q

K

-

A

T

M

M

K

L

I

D

N

T

D

P

R

G

P

P

V

Y

I

L

M

L

D

D

F

V

V

I

V

C

D

P

R
x
H

E

Q

E

E

N

H

V

V

L

L

P

P

M

M

V

I

P

P

A
x
S

G

G

K

G

S

T

Y

F

R

N

E

D

M

V

I

I

active site: Y33 (≠ L25), G35 (= G27), G36 (= G28), A37 (= A29), S38 (≠ I30), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), M266 (= M261), V293 (= V288), V400 (= V395), G426 (= G421), M428 (= M423), D453 (= D448), N480 (= N475), H482 (≠ F477), L483 (= L478), M485 (= M480), V486 (= V481), W489 (= W484), H558 (≠ R547)
binding flavin-adenine dinucleotide: R161 (= R152), G222 (= G215), G223 (= G216), G224 (= G217), T246 (= T241), L247 (≠ N242), M248 (= M243), M263 (= M258), L264 (= L259), G286 (= G281), R288 (= R283), V293 (= V288), D310 (= D305), I311 (= I306), D329 (= D324), V330 (= V325), M405 (= M400), G423 (= G418), G424 (= G419)
binding magnesium ion: D453 (= D448), N480 (= N475), H482 (≠ F477)
binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: M266 (= M261), D291 (= D286), R292 (= R287), M485 (= M480), W489 (= W484), S568 (≠ A557)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V395), G401 (= G396), Q402 (= Q397), H403 (= H398), M428 (= M423), D453 (= D448), G454 (= G449), S455 (= S450), M458 (= M453), N480 (= N475), H482 (≠ F477), L483 (= L478), G484 (= G479), M485 (= M480), V486 (= V481)

5k6tA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, propoxycarbazone-sodium (see paper)
48% identity, 97% coverage: 4:565/581 of query aligns to 12:576/582 of 5k6tA

query
sites
5k6tA

K

R

R

K

G

G

A

A

D

D

I

I

V

L

I

V

D

E

V

A

L

L

L

E

E

R

E

Q

G

G

V

V

D

E

T

T

V

V

F

F

G

A

L
x
Y

P

P

G
|
G

A
|
A

I
x
S

M

M

E

E

V

I

Y

H

D

Q

A

A

L

L

F

T

-

R

D

S

A

S

P

S

L

I

R

R

N

N

I

V

L

L

A

P

R

R

H

H

E
|
E

Q

Q

A

G

A

V

H

F

M

A

A

A

D

E

G

G

Y

Y

A

A

R

R

A

S

T

S

G

G

K

K

V

P

G

G

V

I

V

C

L

I

A

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

G

G

I

L

A

A

T

D

A

A

Y

L

M

L

D

D

S

S

V

V

P

P

L

L

V

V

A

A

I

I

T

T

G

G

Q

Q

V

V

P

P

T

R

S

R

Y

M

I

I

G

G

T

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

A

T

D

P

V

I

V

V

G

E

I

V

T

T

R

R

P

S

I

I

T

T

K

K

H

H

N

N

F

Y

L

L

V

V

T

M

D

D

I

V

K

E

D

D

L

I

A

P

L

R

I

I

L

I

R

E

E

E

A

A

F

F

Y

F

L

L

A

A

R

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

V

V

D

D

I

V

P

P

K
|
K

D

D

I

I

T

Q

Q

Q

E

-

Y

-

D

-

Y

L

K

A

M

I

P

P

T

N

E

W

K

E

D

Q

V

A

L

M

D

-

A

R

L

L

P

P

G

G

Y

Y

K

M

P

S

H

R

Y

M

E

P

G

K

N

P

P

P

-

E

-

D

F

S

Q

H

I

L

K

E

K

Q

A

I

A

V

E

R

L

L

I

I

R

S

K

E

A

S

K

K

R

K

P

P

V

V

L

L

Y

Y

V

V

G
|
G

T

C

V

L

I

N

S

S

G

-

A

-

S

S

E

D

E

E

L

L

R

G

E

R

L

F

A

V

E

E

L

L

T

T

R

G

I

I

P

P

V

V

T

A

T

S

T
|
T

N
x
L

M
|
M

A

G

K

L

G

G

A

S

F

Y

D

P

E

C

T

D

H

D

P

E

L

L

A

S

L

L

H

H

M

M

L
|
L

G

G

M
|
M

H
|
H

G

G

T

T

Y

V

Y

Y

A

A

N

N

M

Y

A

A

V

V

Y

E

H

H

S

S

D

D

L

L

I

L

A

A

V

F

G
|
G

A

V

R
|
R

F

F

D
|
D

D

D

R
|
R

V
|
V

T

T

G

G

K

K

I

L

Q

E

E

A

F

F

A

A

P

S

E

R

A

A

K

K

I

I

I

V

H

H

I

I

D
|
D

I
|
I

D

D

P

S

A

A

S

E

I

I

S

G

K

K

N

N

I

K

T

T

V

P

D

H

V

V

P

S

I

V

V

C

G

G

D
|
D

V
|
V

K

K

V

L

V

A

L

L

G

Q

K

G

L

M

L

N

K

K

E

V

L

L

K

E

K

N

K

R

P

A

I

E

E

E

W

L

V

K

K

L

A

D

R

F

E

G

S

V

W

W

L

R

K

N

Q

E

I

L

E

N

K

V

W

Q

K

K

E

Q

K

K

H

F

P

P

L

L

T

S

Y

F

D

K

R

T

T

F

S

G

K

E

I

A

I

I

K

P

P

P

Q

Q

Y

Y

V

A

I

I

E

K

E

V

I

L

Y

D

K

E

I

L

T

T

D

D

G

G

E

K

A

A

L

I

I

I

T

S

A

T

G

G

V
|
V

G
|
G

Q
|
Q

H
|
H

Q
|
Q

M
|
M

W

W

A

A

M

Q

F

F

Y

Y

K

N

Y

Y

R

K

Y

K

P

P

R

R

Q

Q

F

W

L

L

N

S

S

S

G
|
G

G

G

L

L

G
|
G

T

A

M
|
M

G

G

Y

F

G

G

F

L

P

P

A

A

V

I

G

G

A

A

K

S

I

V

G

A

N

N

P

P

D

D

K

A

T

I

V

V

F

V

A

D

I

I

E

D

G
|
G

D
|
D

G
|
G

S
|
S

F

F

V

I

M

M

N

N

M

V

Q

Q

D

E

V

L

I

A

T

T

A

I

V

R

Q

V

Y

E

R

N

I

L

P

P

V

V

K

K

I

V

A

L

I

L

N

N

N
|
N

E

Q

F
x
H

L
|
L

G
|
G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

Q

D

L

R

F

F

Y

Y

D

K

S

A

R

N

Y

R

S

A

S

H

V

T

C

F

L

L

A

G

-

D

-

P

-

A

-

Q

-

E

-

D

-

E

V

I

H

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

S

A

M

C

G

G

A

I

V

P

G

A

L

A

R

R

A

V

T

T

K

K

P

K

E

A

E

D

V

L

R

R

P

E

V

A

L

I

E

Q

K

-

A

T

M

M

K

L

I

D

N

T

D

P

R

G

P

P

V

Y

I

L

M

L

D

D

F

V

V

I

V

C

D

P

R
x
H

E

Q

E

E

N

H

V

V

L

L

P

P

M

M

V

I

P

P

A
x
S

G

G

K

G

S

T

Y

F

R

N

E

D

M

V

I

I

active site: Y33 (≠ L25), G35 (= G27), G36 (= G28), A37 (= A29), S38 (≠ I30), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), M266 (= M261), V293 (= V288), V400 (= V395), G426 (= G421), M428 (= M423), D453 (= D448), N480 (= N475), H482 (≠ F477), L483 (= L478), M485 (= M480), V486 (= V481), W489 (= W484), H558 (≠ R547)
binding methyl 2-[(4-methyl-5-oxidanylidene-3-propoxy-1,2,4-triazol-1-yl)carbonylsulfamoyl]benzoate: H267 (= H262), R292 (= R287), M485 (= M480), W489 (= W484), S568 (≠ A557)
binding flavin-adenine dinucleotide: R161 (= R152), G222 (= G215), G223 (= G216), G224 (= G217), T246 (= T241), L247 (≠ N242), M248 (= M243), L264 (= L259), G286 (= G281), R288 (= R283), D290 (= D285), R292 (= R287), V293 (= V288), D310 (= D305), I311 (= I306), D329 (= D324), V330 (= V325), Q404 (= Q399), M405 (= M400), G423 (= G418)
binding magnesium ion: D453 (= D448), N480 (= N475), H482 (≠ F477)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (= V395), G401 (= G396), Q402 (= Q397), H403 (= H398), G426 (= G421), M428 (= M423), G452 (= G447), G454 (= G449), S455 (= S450), N480 (= N475), H482 (≠ F477), L483 (= L478), G484 (= G479)

5k6rA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, thiencarbazone-methyl (see paper)
48% identity, 97% coverage: 4:565/581 of query aligns to 12:576/582 of 5k6rA

query
sites
5k6rA

K

R

R

K

G

G

A

A

D

D

I

I

V

L

I

V

D

E

V

A

L

L

L

E

E

R

E

Q

G

G

V

V

D

E

T

T

V

V

F

F

G

A

L
x
Y

P

P

G
|
G

A
|
A

I
x
S

M

M

E

E

V

I

Y

H

D

Q

A

A

L

L

F

T

-

R

D

S

A

S

P

S

L

I

R

R

N

N

I

V

L

L

A

P

R

R

H

H

E
|
E

Q

Q

A

G

A

V

H

F

M

A

A

A

D

E

G

G

Y

Y

A

A

R

R

A

S

T

S

G

G

K

K

V

P

G

G

V

I

V

C

L

I

A

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

G

G

I

L

A

A

T

D

A

A

Y

L

M

L

D

D

S

S

V

V

P

P

L

L

V

V

A

A

I

I

T

T

G

G

Q

Q

V

V

P

P

T

R

S

R

Y

M

I

I

G

G

T

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

A

T

D

P

V

I

V

V

G

E

I

V

T

T

R

R

P

S

I

I

T

T

K

K

H

H

N

N

F

Y

L

L

V

V

T

M

D

D

I

V

K

E

D

D

L

I

A

P

L

R

I

I

L

I

R

E

E

E

A

A

F

F

Y

F

L

L

A

A

R

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

V

V

D

D

I

V

P

P

K
|
K

D

D

I

I

T

Q

Q

Q

E

-

Y

-

D

-

Y

L

K

A

M

I

P

P

T

N

E

W

K

E

D

Q

V

A

L

M

D

-

A

R

L

L

P

P

G

G

Y

Y

K

M

P

S

H

R

Y

M

E

P

G

K

N

P

P

P

-

E

-

D

F

S

Q

H

I

L

K

E

K

Q

A

I

A

V

E

R

L

L

I

I

R

S

K

E

A

S

K

K

R

K

P

P

V

V

L

L

Y

Y

V

V

G
|
G

T

C

V

L

I

N

S

S

G

-

A

-

S

S

E

D

E

E

L

L

R

G

E

R

L

F

A

V

E

E

L

L

T

T

R

G

I

I

P

P

V

V

T

A

T

S

T
|
T

N
x
L

M
|
M

A

G

K

L

G

G

A

S

F

Y

D

P

E

C

T

D

H

D

P

E

L

L

A

S

L

L

H

H

M

M

L
|
L

G

G

M
|
M

H

H

G

G

T

T

Y

V

Y

Y

A

A

N

N

M

Y

A

A

V

V

Y

E

H

H

S

S

D

D

L

L

I

L

A

A

V

F

G
|
G

A

V

R
|
R

F

F

D

D

R
|
R

V
|
V

T

T

G

G

K

K

I

L

Q

E

E

A

F

F

A

A

P

S

E

R

A

A

K

K

I

I

I

V

H

H

I

I

D
|
D

I
|
I

D

D

P

S

A

A

S

E

I

I

S

G

K

K

N

N

I

K

T

T

V

P

D

H

V

V

P

S

I

V

V

C

G
|
G

D
|
D

V
|
V

K

K

V

L

V

A

L

L

G

Q

K

G

L

M

L

N

K

K

E

V

L

L

K

E

K

N

K

R

P

A

I

E

E

E

W

L

V

K

K

L

A

D

R

F

E

G

S

V

W

W

L

R

K

N

Q

E

I

L

E

N

K

V

W

Q

K

K

E

Q

K

K

H

F

P

P

L

L

T

S

Y

F

D

K

R

T

T

F

S

G

K

E

I

A

I

I

K

P

P

P

Q

Q

Y

Y

V

A

I

I

E

K

E

V

I

L

Y

D

K

E

I

L

T

T

D

D

G

G

E

K

A

A

L

I

I

I

T

S

A

T

G

G

V
|
V

G
|
G

Q
|
Q

H
|
H

Q

Q

M
|
M

W

W

A

A

M

Q

F

F

Y

Y

K

N

Y

Y

R

K

Y

K

P

P

R

R

Q

Q

F

W

L

L

N

S

S

S

G
|
G

G

G

L

L

G
|
G

T

A

M
|
M

G

G

Y

F

G

G

F

L

P

P

A

A

V

I

G

G

A

A

K

S

I

V

G

A

N

N

P

P

D

D

K

A

T

I

V

V

F

V

A

D

I

I

E

D

G

G

D
|
D

G
|
G

S
|
S

F

F

V

I

M
|
M

N

N

M

V

Q

Q

D

E

V

L

I

A

T

T

A

I

V

R

Q

V

Y

E

R

N

I

L

P

P

V

V

K

K

I

V

A

L

I

L

N

N

N
|
N

E

Q

F
x
H

L
|
L

G
|
G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

Q

D

L

R

F

F

Y

Y

D

K

S

A

R

N

Y

R

S

A

S

H

V

T

C

F

L

L

A

G

-

D

-

P

-

A

-

Q

-

E

-

D

-

E

V

I

H

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

S

A

M

C

G

G

A

I

V

P

G

A

L

A

R

R

A

V

T

T

K

K

P

K

E

A

E

D

V

L

R

R

P

E

V

A

L

I

E

Q

K

-

A

T

M

M

K

L

I

D

N

T

D

P

R

G

P

P

V

Y

I

L

M

L

D

D

F

V

V

I

V

C

D

P

R
x
H

E

Q

E

E

N

H

V

V

L

L

P

P

M

M

V

I

P

P

A
x
S

G

G

K

G

S

T

Y

F

R

N

E

D

M

V

I

I

active site: Y33 (≠ L25), G35 (= G27), G36 (= G28), A37 (= A29), S38 (≠ I30), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), M266 (= M261), V293 (= V288), V400 (= V395), G426 (= G421), M428 (= M423), D453 (= D448), N480 (= N475), H482 (≠ F477), L483 (= L478), M485 (= M480), V486 (= V481), W489 (= W484), H558 (≠ R547)
binding methyl 4-[(3-methoxy-4-methyl-5-oxidanylidene-1,2,4-triazol-1-yl)carbonylsulfamoyl]-5-methyl-thiophene-3-carboxylate: R292 (= R287), W489 (= W484), S568 (≠ A557)
binding flavin-adenine dinucleotide: R161 (= R152), G222 (= G215), G223 (= G216), G224 (= G217), T246 (= T241), L247 (≠ N242), M248 (= M243), L264 (= L259), M266 (= M261), G286 (= G281), R288 (= R283), R292 (= R287), V293 (= V288), D310 (= D305), I311 (= I306), G328 (= G323), D329 (= D324), V330 (= V325), M405 (= M400), G423 (= G418)
binding magnesium ion: D453 (= D448), N480 (= N475), H482 (≠ F477)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (= V395), G401 (= G396), Q402 (= Q397), H403 (= H398), G426 (= G421), M428 (= M423), D453 (= D448), G454 (= G449), S455 (= S450), M458 (= M453), N480 (= N475), H482 (≠ F477), L483 (= L478), G484 (= G479), M485 (= M480), V486 (= V481)

1z8nA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with an imidazolinone herbicide, imazaquin (see paper)
48% identity, 97% coverage: 4:565/581 of query aligns to 12:576/582 of 1z8nA

query
sites
1z8nA

K

R

R

K

G

G

A

A

D

D

I

I

V

L

I

V

D

E

V

A

L

L

L

E

E

R

E

Q

G

G

V

V

D

E

T

T

V

V

F

F

G

A

L
x
Y

P

P

G
|
G

A
|
A

I
x
S

M

M

E

E

V

I

Y

H

D

Q

A

A

L

L

F

T

-

R

D

S

A

S

P

S

L

I

R

R

N

N

I

V

L

L

A

P

R

R

H

H

E
|
E

Q

Q

A

G

A

V

H

F

M

A

A

A

D

E

G

G

Y

Y

A

A

R

R

A

S

T

S

G

G

K

K

V

P

G

G

V

I

V

C

L

I

A

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

G

G

I

L

A

A

T

D

A

A

Y

L

M

L

D

D

S

S

V

V

P

P

L

L

V

V

A

A

I

I

T

T

G

G

Q

Q

V

V

P

P

T

R

S

R

Y

M

I

I

G

G

T

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

A

T

D

P

V

I

V

V

G

E

I

V

T

T

R

R

P

S

I

I

T

T

K
|
K

H

H

N

N

F

Y

L

L

V

V

T

M

D

D

I

V

K

E

D

D

L

I

A

P

L

R

I

I

L

I

R

E

E

E

A

A

F

F

Y

F

L

L

A

A

R

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

V

V

D

D

I

V

P

P

K
|
K

D

D

I

I

T

Q

Q

Q

E

-

Y

-

D

-

Y

L

K

A

M

I

P

P

T

N

E

W

K

E

D

Q

V

A

L

M

D

-

A

R

L

L

P

P

G

G

Y
|
Y

K

M

P

S

H
x
R

Y

M

E

P

G

K

N

P

P

P

-

E

-

D

F

S

Q

H

I

L

K

E

K

Q

A

I

A

V

E

R

L

L

I

I

R

S

K

E

A

S

K

K

R

K

P

P

V

V

L

L

Y

Y

V

V

G
|
G

G

G

G
|
G

T

C

V

L

I

N

S

S

G

-

A

-

S

S

E

D

E

E

L

L

R

G

E

R

L

F

A

V

E

E

L

L

T

T

R

G

I

I

P

P

V

V

T

A

T

S

T
|
T

N
x
L

M
|
M

A

G

K

L

G

G

A

S

F

Y

D

P

E

C

T

D

H

D

P

E

L

L

A

S

L

L

H

H

M

M

L
|
L

G
|
G

M
|
M

H
|
H

G

G

T

T

Y

V

Y

Y

A

A

N

N

M

Y

A

A

V

V

Y

E

H

H

S

S

D

D

L

L

I

L

A

A

V

F

G
|
G

A
x
V

R
|
R

F

F

D
|
D

R
|
R

V
|
V

T

T

G

G

K

K

I

L

Q

E

E

A

F

F

A

A

P

S

E

R

A

A

K

K

I

I

I

V

H

H

I

I

D
|
D

I
|
I

D
|
D

P

S

A

A

S

E

I

I

S

G

K

K

N

N

I

K

T

T

V

P

D

H

V

V

P

S

I

V

V

C

G

G

D
|
D

V
|
V

K

K

V

L

V

A

L

L

G

Q

K

G

L

M

L

N

K

K

E

V

L

L

K

E

K

N

K

R

P

A

I

E

E

E

W

L

V

K

K

L

A

D

R

F

E

G

S

V

W

W

L

R

K

N

Q

E

I

L

E

N

K

V

W

Q

K

K

E

Q

K

K

H

F

P

P

L

L

T

S

Y

F

D

K

R

T

T

F

S

G

K

E

I

A

I

I

K

P

P

P

Q

Q

Y

Y

V

A

I

I

E

K

E

V

I

L

Y

D

K

E

I

L

T

T

D

D

G

G

E

K

A

A

L

I

I

I

T

S

A

T

G

G

V
|
V

G
|
G

Q
|
Q

H
|
H

Q

Q

M
|
M

W

W

A

A

M

Q

F

F

Y

Y

K

N

Y

Y

R

K

Y

K

P

P

R

R

Q

Q

F

W

L

L

N

S

S

S

G
|
G

L

L

G
|
G

T

A

M
|
M

G

G

Y

F

G

G

F

L

P

P

A

A

V

I

G

G

A

A

K

S

I

V

G

A

N

N

P

P

D

D

K

A

T

I

V

V

F

V

A

D

I

I

E

D

G
|
G

D
|
D

G
|
G

S
|
S

F

F

V

I

M

M

N

N

M

V

Q

Q

D

E

V

L

I

A

T

T

A

I

V

R

Q

V

Y

E

R

N

I

L

P

P

V

V

K

K

I

V

A

L

I

L

N

N

N
|
N

E

Q

F
x
H

L
|
L

G
|
G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

Q

D

L

R

F

F

Y

Y

D

K

S

A

R

N

Y

R

S

A

S

H

V

T

C

F

L

L

A

G

-

D

-

P

-

A

-

Q

-

E

-

D

-

E

V

I

H

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

S

A

M

C

G

G

A

I

V

P

G

A

L

A

R

R

A

V

T

T

K

K

P

K

E

A

E

D

V

L

R

R

P

E

V

A

L

I

E

Q

K

-

A

T

M

M

K

L

I

D

N

T

D

P

R

G

P

P

V

Y

I

L

M

L

D

D

F

V

V

I

V

C

D

P

R
x
H

E

Q

E

E

N

H

V

V

L

L

P

P

M

M

V

I

P

P

A

S

G
|
G

K

G

S

T

Y

F

R

N

E

D

M

V

I

I

active site: Y33 (≠ L25), G35 (= G27), G36 (= G28), A37 (= A29), S38 (≠ I30), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), M266 (= M261), V293 (= V288), V400 (= V395), G426 (= G421), M428 (= M423), D453 (= D448), N480 (= N475), H482 (≠ F477), L483 (= L478), M485 (= M480), V486 (= V481), W489 (= W484), H558 (≠ R547)
binding 2-(4-isopropyl-4-methyl-5-oxo-4,5-dihydro-1h-imidazol-2-yl)quinoline-3-carboxylic acid: K135 (= K126), R161 (= R152), Y191 (= Y186), R194 (≠ H189), D291 (= D286), R292 (= R287), D312 (= D307), W489 (= W484), G569 (= G558)
binding flavin-adenine dinucleotide: R161 (= R152), G222 (= G215), G224 (= G217), T246 (= T241), L247 (≠ N242), M248 (= M243), L264 (= L259), G265 (= G260), M266 (= M261), H267 (= H262), G286 (= G281), V287 (≠ A282), R288 (= R283), D290 (= D285), R292 (= R287), V293 (= V288), D310 (= D305), I311 (= I306), D329 (= D324), V330 (= V325), M405 (= M400), G423 (= G418), G424 (= G419)
binding magnesium ion: D453 (= D448), N480 (= N475)
binding thiamine diphosphate: V400 (= V395), G401 (= G396), Q402 (= Q397), H403 (= H398), G426 (= G421), M428 (= M423), G452 (= G447), G454 (= G449), S455 (= S450), N480 (= N475), H482 (≠ F477), L483 (= L478), G484 (= G479), M485 (= M480), V486 (= V481)

1yi1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, tribenuron methyl (see paper)
48% identity, 97% coverage: 4:565/581 of query aligns to 12:576/582 of 1yi1A

query
sites
1yi1A

K

R

R

K

G

G

A

A

D

D

I

I

V

L

I

V

D

E

V

A

L

L

L

E

E

R

E

Q

G

G

V

V

D

E

T

T

V

V

F

F

G

A

L
x
Y

P

P

G
|
G

A
|
A

I
x
S

M

M

E

E

V

I

Y

H

D

Q

A

A

L

L

F

T

-

R

D

S

A

S

P

S

L

I

R

R

N

N

I

V

L

L

A

P

R

R

H

H

E
|
E

Q

Q

A

G

A

V

H

F

M

A

A

A

D

E

G

G

Y

Y

A

A

R

R

A

S

T

S

G

G

K

K

V

P

G

G

V

I

V

C

L

I

A

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

G

G

I

L

A

A

T

D

A

A

Y

L

M

L

D

D

S

S

V

V

P

P

L

L

V

V

A

A

I

I

T

T

G

G

Q

Q

V

V

P

P

T

R

S

R

Y

M

I

I

G

G

T

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

A

T

D

P

V

I

V

V

G

E

I

V

T

T

R

R

P

S

I

I

T

T

K

K

H

H

N

N

F

Y

L

L

V

V

T

M

D

D

I

V

K

E

D

D

L

I

A

P

L

R

I

I

L

I

R

E

E

E

A

A

F

F

Y

F

L

L

A

A

R

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

V

V

D

D

I

V

P

P

K
|
K

D

D

I

I

T

Q

Q

Q

E

-

Y

-

D

-

Y

L

K

A

M

I

P

P

T

N

E

W

K

E

D

Q

V

A

L

M

D

-

A

R

L

L

P

P

G

G

Y

Y

K

M

P

S

H

R

Y

M

E

P

G

K

N

P

P

P

-

E

-

D

F

S

Q

H

I

L

K

E

K

Q

A

I

A

V

E

R

L

L

I

I

R

S

K

E

A

S

K

K

R

K

P

P

V

V

L

L

Y

Y

V

V

G

G

G
|
G

T

C

V

L

I

N

S

S

G

-

A

-

S

S

E

D

E

E

L

L

R

G

E

R

L

F

A

V

E

E

L

L

T

T

R

G

I

I

P

P

V

V

T

A

T

S

T
|
T

N
x
L

M
|
M

A

G

K

L

G

G

A

S

F

Y

D

P

E

C

T

D

H

D

P

E

L

L

A

S

L

L

H

H

M
|
M

L
|
L

G
|
G

M
|
M

H
|
H

G

G

T

T

Y

V

Y

Y

A

A

N

N

M

Y

A

A

V

V

Y

E

H

H

S

S

D

D

L

L

I

L

A

A

V

F

G
|
G

A
x
V

R
|
R

F

F

D
|
D

R
|
R

V
|
V

T

T

G

G

K

K

I

L

Q

E

E

A

F

F

A

A

P

S

E

R

A

A

K

K

I

I

I

V

H

H

I

I

D
|
D

I
|
I

D

D

P

S

A

A

S

E

I

I

S

G

K

K

N

N

I

K

T

T

V

P

D

H

V

V

P

S

I

V

V

C

G

G

D
|
D

V
|
V

K

K

V

L

V

A

L

L

G

Q

K

G

L

M

L

N

K

K

E

V

L

L

K

E

K

N

K

R

P

A

I

E

E

E

W

L

V

K

K

L

A

D

R

F

E

G

S

V

W

W

L

R

K

N

Q

E

I

L

E

N

K

V

W

Q

K

K

E

Q

K

K

H

F

P

P

L

L

T

S

Y

F

D

K

R

T

T

F

S

G

K

E

I

A

I

I

K

P

P

P

Q

Q

Y

Y

V

A

I

I

E

K

E

V

I

L

Y

D

K

E

I

L

T

T

D

D

G

G

E

K

A

A

L

I

I

I

T

S

A

T

G

G

V
|
V

G

G

Q

Q

H

H

Q

Q

M
|
M

W

W

A

A

M

Q

F

F

Y

Y

K

N

Y

Y

R

K

Y

K

P

P

R

R

Q

Q

F

W

L

L

N

S

S

S

G
|
G

L

L

G
|
G

T

A

M
|
M

G

G

Y

F

G

G

F

L

P

P

A

A

V

I

G

G

A

A

K

S

I

V

G

A

N

N

P

P

D

D

K

A

T

I

V

V

F

V

A

D

I

I

E

D

G

G

D
|
D

G

G

S

S

F

F

V

I

M

M

N

N

M

V

Q

Q

D

E

V

L

I

A

T

T

A

I

V

R

Q

V

Y

E

R

N

I

L

P

P

V

V

K

K

I

V

A

L

I

L

N

N

N
|
N

E

Q

F
x
H

L
|
L

G

G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

Q

D

L

R

F

F

Y

Y

D

K

S

A

R

N

Y

R

S

A

S

H

V

T

C

F

L

L

A

G

-

D

-

P

-

A

-

Q

-

E

-

D

-

E

V

I

H

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

S

A

M

C

G

G

A

I

V

P

G

A

L

A

R

R

A

V

T

T

K

K

P

K

E

A

E

D

V

L

R

R

P

E

V

A

L

I

E

Q

K

-

A

T

M

M

K

L

I

D

N

T

D

P

R

G

P

P

V

Y

I

L

M

L

D

D

F

V

V

I

V

C

D

P

R
x
H

E

Q

E

E

N

H

V

V

L

L

P

P

M

M

V

I

P

P

A
x
S

G

G

K

G

S

T

Y

F

R

N

E

D

M

V

I

I

active site: Y33 (≠ L25), G35 (= G27), G36 (= G28), A37 (= A29), S38 (≠ I30), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), M266 (= M261), V293 (= V288), V400 (= V395), G426 (= G421), M428 (= M423), D453 (= D448), N480 (= N475), H482 (≠ F477), L483 (= L478), M485 (= M480), V486 (= V481), W489 (= W484), H558 (≠ R547)
binding methyl 2-[4-methoxy-6-methyl-1,3,5-trazin-2-yl(methyl)carbamoylsulfamoyl]benzoate: D291 (= D286), R292 (= R287), W489 (= W484), S568 (≠ A557)
binding flavin-adenine dinucleotide: R161 (= R152), G223 (= G216), G224 (= G217), T246 (= T241), L247 (≠ N242), M248 (= M243), M263 (= M258), L264 (= L259), G265 (= G260), M266 (= M261), H267 (= H262), G286 (= G281), V287 (≠ A282), R288 (= R283), D290 (= D285), V293 (= V288), D310 (= D305), I311 (= I306), D329 (= D324), V330 (= V325), M405 (= M400), G423 (= G418), G424 (= G419)
binding magnesium ion: D453 (= D448), N480 (= N475), H482 (≠ F477)

1yi0A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, sulfometuron methyl (see paper)
48% identity, 97% coverage: 4:565/581 of query aligns to 12:576/582 of 1yi0A

query
sites
1yi0A

K

R

R

K

G

G

A

A

D

D

I

I

V

L

I

V

D

E

V

A

L

L

L

E

E

R

E

Q

G

G

V

V

D

E

T

T

V

V

F

F

G

A

L
x
Y

P

P

G
|
G

A
|
A

I
x
S

M

M

E

E

V

I

Y

H

D

Q

A

A

L

L

F

T

-

R

D

S

A

S

P

S

L

I

R

R

N

N

I

V

L

L

A

P

R

R

H

H

E
|
E

Q

Q

A

G

A

V

H

F

M

A

A

A

D

E

G

G

Y

Y

A

A

R

R

A

S

T

S

G

G

K

K

V

P

G

G

V

I

V

C

L

I

A

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

G

G

I

L

A

A

T

D

A

A

Y

L

M

L

D

D

S

S

V

V

P

P

L

L

V

V

A

A

I

I

T

T

G

G

Q

Q

V

V

P

P

T

R

S

R

Y

M

I

I

G

G

T

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

A

T

D

P

V

I

V

V

G

E

I

V

T

T

R

R

P

S

I

I

T

T

K

K

H

H

N

N

F

Y

L

L

V

V

T

M

D

D

I

V

K

E

D

D

L

I

A

P

L

R

I

I

L

I

R

E

E

E

A

A

F

F

Y

F

L

L

A

A

R

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

V

V

D

D

I

V

P

P

K
|
K

D

D

I

I

T

Q

Q

Q

E

-

Y

-

D

-

Y

L

K

A

M

I

P

P

T

N

E

W

K

E

D

Q

V

A

L

M

D

-

A

R

L

L

P

P

G

G

Y

Y

K

M

P

S

H

R

Y

M

E

P

G

K

N

P

P

P

-

E

-

D

F

S

Q

H

I

L

K

E

K

Q

A

I

A

V

E

R

L

L

I

I

R

S

K

E

A

S

K

K

R

K

P

P

V

V

L

L

Y

Y

V

V

G
|
G

T

C

V

L

I

N

S

S

G

-

A

-

S

S

E

D

E

E

L

L

R

G

E

R

L

F

A

V

E

E

L

L

T

T

R

G

I

I

P

P

V

V

T

A

T

S

T
|
T

N
x
L

M
|
M

A

G

K

L

G

G

A

S

F

Y

D

P

E

C

T

D

H

D

P

E

L

L

A

S

L

L

H

H

M

M

L
|
L

G
|
G

M
|
M

H
|
H

G

G

T

T

Y

V

Y

Y

A

A

N

N

M

Y

A

A

V

V

Y

E

H

H

S

S

D

D

L

L

I

L

A

A

V

F

G
|
G

A
x
V

R
|
R

F

F

D
|
D

R
|
R

V
|
V

T

T

G

G

K

K

I

L

Q

E

E

A

F

F

A

A

P

S

E

R

A

A

K

K

I

I

I

V

H

H

I

I

D
|
D

I
|
I

D

D

P

S

A

A

S

E

I

I

S

G

K

K

N

N

I

K

T

T

V

P

D

H

V

V

P

S

I

V

V

C

G
|
G

D
|
D

V
|
V

K

K

V

L

V

A

L

L

G

Q

K

G

L

M

L

N

K

K

E

V

L

L

K

E

K

N

K

R

P

A

I

E

E

E

W

L

V

K

K

L

A

D

R

F

E

G

S

V

W

W

L

R

K

N

Q

E

I

L

E

N

K

V

W

Q

K

K

E

Q

K

K

H

F

P

P

L

L

T

S

Y

F

D

K

R

T

T

F

S

G

K

E

I

A

I

I

K

P

P

P

Q

Q

Y

Y

V

A

I

I

E

K

E

V

I

L

Y

D

K

E

I

L

T

T

D

D

G

G

E

K

A

A

L

I

I

I

T

S

A

T

G

G

V
|
V

G

G

Q

Q

H

H

Q

Q

M
|
M

W

W

A

A

M

Q

F

F

Y

Y

K

N

Y

Y

R

K

Y

K

P

P

R

R

Q

Q

F

W

L

L

N

S

S

S

G
|
G

L

L

G
|
G

T

A

M
|
M

G

G

Y

F

G

G

F

L

P

P

A

A

V

I

G

G

A

A

K

S

I

V

G

A

N

N

P

P

D

D

K

A

T

I

V

V

F

V

A

D

I

I

E

D

G

G

D
|
D

G

G

S

S

F

F

V

I

M

M

N

N

M

V

Q

Q

D

E

V

L

I

A

T

T

A

I

V

R

Q

V

Y

E

R

N

I

L

P

P

V

V

K

K

I

V

A

L

I

L

N

N

N
|
N

E

Q

F
x
H

L
|
L

G

G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

Q

D

L

R

F

F

Y

Y

D

K

S

A

R

N

Y

R

S

A

S

H

V

T

C

F

L

L

A

G

-

D

-

P

-

A

-

Q

-

E

-

D

-

E

V

I

H

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

S

A

M

C

G

G

A

I

V

P

G

A

L

A

R

R

A

V

T

T

K

K

P

K

E

A

E

D

V

L

R

R

P

E

V

A

L

I

E

Q

K

-

A

T

M

M

K

L

I

D

N

T

D

P

R

G

P

P

V

Y

I

L

M

L

D

D

F

V

V

I

V

C

D

P

R
x
H

E

Q

E

E

N

H

V

V

L

L

P

P

M

M

V

I

P

P

A
x
S

G

G

K

G

S

T

Y

F

R

N

E

D

M

V

I

I

active site: Y33 (≠ L25), G35 (= G27), G36 (= G28), A37 (= A29), S38 (≠ I30), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), M266 (= M261), V293 (= V288), V400 (= V395), G426 (= G421), M428 (= M423), D453 (= D448), N480 (= N475), H482 (≠ F477), L483 (= L478), M485 (= M480), V486 (= V481), W489 (= W484), H558 (≠ R547)
binding methyl 2-[({[(4,6-dimethylpyrimidin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D291 (= D286), R292 (= R287), W489 (= W484), S568 (≠ A557)
binding flavin-adenine dinucleotide: R161 (= R152), G222 (= G215), G223 (= G216), G224 (= G217), T246 (= T241), L247 (≠ N242), M248 (= M243), L264 (= L259), G265 (= G260), M266 (= M261), H267 (= H262), G286 (= G281), V287 (≠ A282), R288 (= R283), D290 (= D285), R292 (= R287), V293 (= V288), D310 (= D305), I311 (= I306), G328 (= G323), D329 (= D324), V330 (= V325), M405 (= M400), G423 (= G418), G424 (= G419)
binding magnesium ion: D453 (= D448), N480 (= N475), H482 (≠ F477)

1yhzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorsulfuron (see paper)
48% identity, 97% coverage: 4:565/581 of query aligns to 12:576/582 of 1yhzA

query
sites
1yhzA

K

R

R

K

G

G

A

A

D

D

I

I

V

L

I

V

D

E

V

A

L

L

L

E

E

R

E

Q

G

G

V

V

D

E

T

T

V

V

F

F

G

A

L
x
Y

P

P

G
|
G

A
|
A

I
x
S

M

M

E

E

V

I

Y

H

D

Q

A

A

L

L

F

T

-

R

D

S

A

S

P

S

L

I

R

R

N

N

I

V

L

L

A

P

R

R

H

H

E
|
E

Q

Q

A

G

A

V

H

F

M

A

A

A

D

E

G

G

Y

Y

A

A

R

R

A

S

T

S

G

G

K

K

V

P

G

G

V

I

V

C

L

I

A

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

G

G

I

L

A

A

T

D

A

A

Y

L

M

L

D

D

S

S

V

V

P

P

L

L

V

V

A

A

I

I

T

T

G

G

Q

Q

V

V

P

P

T

R

S

R

Y

M

I

I

G

G

T

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

A

T

D

P

V

I

V

V

G

E

I

V

T

T

R

R

P

S

I

I

T

T

K

K

H

H

N

N

F

Y

L

L

V

V

T

M

D

D

I

V

K

E

D

D

L

I

A

P

L

R

I

I

L

I

R

E

E

E

A

A

F

F

Y

F

L

L

A

A

R

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

V

V

D

D

I

V

P

P

K
|
K

D

D

I

I

T

Q

Q

Q

E

-

Y

-

D

-

Y

L

K

A

M

I

P

P

T

N

E

W

K

E

D

Q

V

A

L

M

D

-

A

R

L

L

P

P

G

G

Y

Y

K

M

P

S

H

R

Y

M

E

P

G

K

N

P

P

P

-

E

-

D

F

S

Q

H

I

L

K

E

K

Q

A

I

A

V

E

R

L

L

I

I

R

S

K

E

A

S

K

K

R

K

P

P

V

V

L

L

Y

Y

V

V

G

G

G
|
G

T

C

V

L

I

N

S

S

G

-

A

-

S

S

E

D

E

E

L

L

R

G

E

R

L

F

A

V

E

E

L

L

T

T

R

G

I

I

P

P

V

V

T

A

T

S

T
|
T

N
x
L

M
|
M

A

G

K

L

G

G

A

S

F

Y

D

P

E

C

T

D

H

D

P

E

L

L

A

S

L

L

H

H

M

M

L
|
L

G

G

M
|
M

H
|
H

G

G

T

T

Y

V

Y

Y

A

A

N

N

M

Y

A

A

V

V

Y

E

H

H

S

S

D

D

L

L

I

L

A

A

V

F

G
|
G

A
x
V

R
|
R

F

F

D
|
D

R
|
R

V
|
V

T

T

G

G

K

K

I

L

Q

E

E

A

F

F

A

A

P

S

E

R

A

A

K

K

I

I

I

V

H

H

I

I

D
|
D

I
|
I

D

D

P

S

A

A

S

E

I

I

S

G

K

K

N

N

I

K

T

T

V

P

D

H

V

V

P

S

I

V

V

C

G

G

D
|
D

V
|
V

K

K

V

L

V

A

L

L

G

Q

K

G

L

M

L

N

K

K

E

V

L

L

K

E

K

N

K

R

P

A

I

E

E

E

W

L

V

K

K

L

A

D

R

F

E

G

S

V

W

W

L

R

K

N

Q

E

I

L

E

N

K

V

W

Q

K

K

E

Q

K

K

H

F

P

P

L

L

T

S

Y

F

D

K

R

T

T

F

S

G

K

E

I

A

I

I

K

P

P

P

Q

Q

Y

Y

V

A

I

I

E

K

E

V

I

L

Y

D

K

E

I

L

T

T

D

D

G

G

E

K

A

A

L

I

I

I

T

S

A

T

G

G

V
|
V

G

G

Q

Q

H

H

Q
|
Q

M
|
M

W

W

A

A

M

Q

F

F

Y

Y

K

N

Y

Y

R

K

Y

K

P

P

R

R

Q

Q

F

W

L

L

N

S

S

S

G
|
G

L

L

G
|
G

T

A

M
|
M

G

G

Y

F

G

G

F

L

P

P

A

A

V

I

G

G

A

A

K

S

I

V

G

A

N

N

P

P

D

D

K

A

T

I

V

V

F

V

A

D

I

I

E

D

G

G

D
|
D

G

G

S

S

F

F

V

I

M

M

N

N

M

V

Q

Q

D

E

V

L

I

A

T

T

A

I

V

R

Q

V

Y

E

R

N

I

L

P

P

V

V

K

K

I

V

A

L

I

L

N

N

N
|
N

E

Q

F
x
H

L
|
L

G

G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

Q

D

L

R

F

F

Y

Y

D

K

S

A

R

N

Y

R

S

A

S

H

V

T

C

F

L

L

A

G

-

D

-

P

-

A

-

Q

-

E

-

D

-

E

V

I

H

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

S

A

M

C

G

G

A

I

V

P

G

A

L

A

R

R

A

V

T

T

K

K

P

K

E

A

E

D

V

L

R

R

P

E

V

A

L

I

E

Q

K

-

A

T

M

M

K

L

I

D

N

T

D

P

R

G

P

P

V

Y

I

L

M

L

D

D

F

V

V

I

V

C

D

P

R
x
H

E

Q

E

E

N

H

V

V

L

L

P

P

M

M

V

I

P

P

A
x
S

G

G

K

G

S

T

Y

F

R

N

E

D

M

V

I

I

active site: Y33 (≠ L25), G35 (= G27), G36 (= G28), A37 (= A29), S38 (≠ I30), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), M266 (= M261), V293 (= V288), V400 (= V395), G426 (= G421), M428 (= M423), D453 (= D448), N480 (= N475), H482 (≠ F477), L483 (= L478), M485 (= M480), V486 (= V481), W489 (= W484), H558 (≠ R547)
binding 1-(2-chlorophenylsulfonyl)-3-(4-methoxy-6-methyl-l,3,5-triazin-2-yl)urea: D291 (= D286), R292 (= R287), M485 (= M480), W489 (= W484), S568 (≠ A557)
binding flavin-adenine dinucleotide: R161 (= R152), G223 (= G216), G224 (= G217), T246 (= T241), L247 (≠ N242), M248 (= M243), L264 (= L259), M266 (= M261), H267 (= H262), G286 (= G281), V287 (≠ A282), R288 (= R283), D290 (= D285), V293 (= V288), D310 (= D305), I311 (= I306), D329 (= D324), V330 (= V325), Q404 (= Q399), M405 (= M400), G423 (= G418), G424 (= G419)
binding magnesium ion: D453 (= D448), N480 (= N475), H482 (≠ F477)

1yhyA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, metsulfuron methyl (see paper)
48% identity, 97% coverage: 4:565/581 of query aligns to 12:576/582 of 1yhyA

query
sites
1yhyA

K

R

R

K

G

G

A

A

D

D

I

I

V

L

I

V

D

E

V

A

L

L

L

E

E

R

E

Q

G

G

V

V

D

E

T

T

V

V

F

F

G

A

L
x
Y

P

P

G
|
G

A
|
A

I
x
S

M

M

E

E

V

I

Y

H

D

Q

A

A

L

L

F

T

-

R

D

S

A

S

P

S

L

I

R

R

N

N

I

V

L

L

A

P

R

R

H

H

E
|
E

Q

Q

A

G

A

V

H

F

M

A

A

A

D

E

G

G

Y

Y

A

A

R

R

A

S

T

S

G

G

K

K

V

P

G

G

V

I

V

C

L

I

A

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

G

G

I

L

A

A

T

D

A

A

Y

L

M

L

D

D

S

S

V

V

P

P

L

L

V

V

A

A

I

I

T

T

G

G

Q

Q

V

V

P

P

T

R

S

R

Y

M

I

I

G

G

T

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

A

T

D

P

V

I

V

V

G

E

I

V

T

T

R

R

P

S

I

I

T

T

K

K

H

H

N

N

F

Y

L

L

V

V

T

M

D

D

I

V

K

E

D

D

L

I

A

P

L

R

I

I

L

I

R

E

E

E

A

A

F

F

Y

F

L

L

A

A

R

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

V

V

D

D

I

V

P

P

K
|
K

D

D

I

I

T

Q

Q

Q

E

-

Y

-

D

-

Y

L

K

A

M

I

P

P

T

N

E

W

K

E

D

Q

V

A

L

M

D

-

A

R

L

L

P

P

G

G

Y

Y

K

M

P

S

H

R

Y

M

E

P

G

K

N

P

P

P

-

E

-

D

F

S

Q

H

I

L

K

E

K

Q

A

I

A

V

E

R

L

L

I

I

R

S

K

E

A

S

K

K

R

K

P

P

V

V

L

L

Y

Y

V

V

G
|
G

T

C

V

L

I

N

S

S

G

-

A

-

S

S

E

D

E

E

L

L

R

G

E

R

L

F

A

V

E

E

L

L

T

T

R

G

I

I

P

P

V

V

T

A

T

S

T
|
T

N
x
L

M
|
M

A

G

K

L

G

G

A

S

F

Y

D

P

E

C

T

D

H

D

P

E

L

L

A

S

L

L

H

H

M

M

L
|
L

G
|
G

M
|
M

H
|
H

G

G

T

T

Y

V

Y

Y

A

A

N

N

M

Y

A

A

V

V

Y

E

H

H

S

S

D

D

L

L

I

L

A

A

V

F

G
|
G

A
x
V

R
|
R

F

F

D
|
D

R
|
R

V
|
V

T

T

G

G

K

K

I

L

Q

E

E

A

F

F

A

A

P

S

E

R

A

A

K

K

I

I

I

V

H

H

I

I

D
|
D

I
|
I

D

D

P

S

A

A

S

E

I

I

S

G

K

K

N

N

I

K

T

T

V

P

D

H

V

V

P

S

I

V

V

C

G

G

D
|
D

V
|
V

K

K

V

L

V

A

L

L

G

Q

K

G

L

M

L

N

K

K

E

V

L

L

K

E

K

N

K

R

P

A

I

E

E

E

W

L

V

K

K

L

A

D

R

F

E

G

S

V

W

W

L

R

K

N

Q

E

I

L

E

N

K

V

W

Q

K

K

E

Q

K

K

H

F

P

P

L

L

T

S

Y

F

D

K

R

T

T

F

S

G

K

E

I

A

I

I

K

P

P

P

Q

Q

Y

Y

V

A

I

I

E

K

E

V

I

L

Y

D

K

E

I

L

T

T

D

D

G

G

E

K

A

A

L

I

I

I

T

S

A

T

G

G

V
|
V

G

G

Q

Q

H

H

Q
|
Q

M
|
M

W

W

A

A

M

Q

F

F

Y

Y

K

N

Y

Y

R

K

Y

K

P

P

R

R

Q

Q

F

W

L

L

N

S

S

S

G
|
G

L

L

G
|
G

T

A

M
|
M

G

G

Y

F

G

G

F

L

P

P

A

A

V

I

G

G

A

A

K

S

I

V

G

A

N

N

P

P

D

D

K

A

T

I

V

V

F

V

A

D

I

I

E

D

G

G

D
|
D

G

G

S

S

F

F

V

I

M

M

N

N

M

V

Q

Q

D

E

V

L

I

A

T

T

A

I

V

R

Q

V

Y

E

R

N

I

L

P

P

V

V

K

K

I

V

A

L

I

L

N

N

N
|
N

E

Q

F
x
H

L
|
L

G

G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

Q

D

L

R

F

F

Y

Y

D

K

S

A

R

N

Y

R

S

A

S

H

V

T

C

F

L

L

A

G

-

D

-

P

-

A

-

Q

-

E

-

D

-

E

V

I

H

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

S

A

M

C

G

G

A

I

V

P

G

A

L

A

R

R

A

V

T

T

K

K

P

K

E

A

E

D

V

L

R

R

P

E

V

A

L

I

E

Q

K

-

A

T

M

M

K

L

I

D

N

T

D

P

R

G

P

P

V

Y

I

L

M

L

D

D

F

V

V

I

V

C

D

P

R
x
H

E

Q

E

E

N

H

V

V

L

L

P

P

M

M

V

I

P

P

A
x
S

G

G

K

G

S

T

Y

F

R

N

E

D

M

V

I

I

active site: Y33 (≠ L25), G35 (= G27), G36 (= G28), A37 (= A29), S38 (≠ I30), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), M266 (= M261), V293 (= V288), V400 (= V395), G426 (= G421), M428 (= M423), D453 (= D448), N480 (= N475), H482 (≠ F477), L483 (= L478), M485 (= M480), V486 (= V481), W489 (= W484), H558 (≠ R547)
binding methyl 2-[({[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D291 (= D286), R292 (= R287), V486 (= V481), W489 (= W484), S568 (≠ A557)
binding flavin-adenine dinucleotide: R161 (= R152), G222 (= G215), G223 (= G216), G224 (= G217), T246 (= T241), L247 (≠ N242), M248 (= M243), L264 (= L259), G265 (= G260), M266 (= M261), H267 (= H262), G286 (= G281), V287 (≠ A282), R288 (= R283), D290 (= D285), V293 (= V288), D310 (= D305), I311 (= I306), D329 (= D324), V330 (= V325), Q404 (= Q399), M405 (= M400), G423 (= G418), G424 (= G419)
binding magnesium ion: D453 (= D448), N480 (= N475), H482 (≠ F477)

1ybhA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide chlorimuron ethyl (see paper)
48% identity, 97% coverage: 4:565/581 of query aligns to 12:576/582 of 1ybhA

query
sites
1ybhA

K

R

R

K

G

G

A

A

D

D

I

I

V

L

I

V

D

E

V

A

L

L

L

E

E

R

E

Q

G

G

V

V

D

E

T

T

V

V

F

F

G

A

L
x
Y

P

P

G
|
G

A
|
A

I
x
S

M

M

E

E

V

I

Y

H

D

Q

A

A

L

L

F

T

-

R

D

S

A

S

P

S

L

I

R

R

N

N

I

V

L

L

A

P

R

R

H

H

E
|
E

Q

Q

A

G

A

V

H

F

M

A

A

A

D

E

G

G

Y

Y

A

A

R

R

A

S

T

S

G

G

K

K

V

P

G

G

V

I

V

C

L

I

A

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

G

G

I

L

A

A

T

D

A

A

Y

L

M

L

D

D

S

S

V

V

P

P

L

L

V

V

A

A

I

I

T

T

G

G

Q

Q

V

V

P

P

T

R

S

R

Y

M

I

I

G

G

T

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

A

T

D

P

V

I

V

V

G

E

I

V

T

T

R

R

P

S

I

I

T

T

K

K

H

H

N

N

F

Y

L

L

V

V

T

M

D

D

I

V

K

E

D

D

L

I

A

P

L

R

I

I

L

I

R

E

E

E

A

A

F

F

Y

F

L

L

A

A

R

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

V

V

D

D

I

V

P

P

K
|
K

D

D

I

I

T

Q

Q

Q

E

-

Y

-

D

-

Y

L

K

A

M

I

P

P

T

N

E

W

K

E

D

Q

V

A

L

M

D

-

A

R

L

L

P

P

G

G

Y

Y

K

M

P

S

H

R

Y

M

E

P

G

K

N

P

P

P

-

E

-

D

F

S

Q

H

I

L

K

E

K

Q

A

I

A

V

E

R

L

L

I

I

R

S

K

E

A

S

K

K

R

K

P

P

V

V

L

L

Y

Y

V

V

G

G

G
|
G

T

C

V

L

I

N

S

S

G

-

A

-

S

S

E

D

E

E

L

L

R

G

E

R

L

F

A

V

E

E

L

L

T

T

R

G

I

I

P

P

V

V

T

A

T

S

T
|
T

N
x
L

M
|
M

A

G

K

L

G

G

A

S

F

Y

D

P

E

C

T

D

H

D

P

E

L

L

A

S

L

L

H

H

M

M

L
|
L

G

G

M
|
M

H
|
H

G

G

T

T

Y

V

Y

Y

A

A

N

N

M

Y

A

A

V

V

Y

E

H

H

S

S

D

D

L

L

I

L

A

A

V

F

G
|
G

A
x
V

R
|
R

F

F

D
|
D

R
|
R

V
|
V

T

T

G

G

K

K

I

L

Q

E

E

A

F

F

A

A

P

S

E

R

A

A

K

K

I

I

I

V

H

H

I

I

D
|
D

I
|
I

D

D

P

S

A

A

S

E

I

I

S

G

K

K

N

N

I

K

T

T

V

P

D

H

V

V

P

S

I

V

V

C

G

G

D
|
D

V
|
V

K

K

V

L

V

A

L

L

G

Q

K

G

L

M

L

N

K

K

E

V

L

L

K

E

K

N

K

R

P

A

I

E

E

E

W

L

V

K

K

L

A

D

R

F

E

G

S

V

W

W

L

R

K

N

Q

E

I

L

E

N

K

V

W

Q

K

K

E

Q

K

K

H

F

P

P

L

L

T

S

Y

F

D

K

R

T

T

F

S

G

K

E

I

A

I

I

K

P

P

P

Q

Q

Y

Y

V

A

I

I

E

K

E

V

I

L

Y

D

K

E

I

L

T

T

D

D

G

G

E

K

A

A

L

I

I

I

T

S

A

T

G

G

V
|
V

G

G

Q

Q

H

H

Q
|
Q

M
|
M

W

W

A

A

M

Q

F

F

Y

Y

K

N

Y

Y

R

K

Y

K

P

P

R

R

Q

Q

F

W

L

L

N

S

S

S

G
|
G

L

L

G
|
G

T

A

M
|
M

G

G

Y

F

G

G

F

L

P

P

A

A

V

I

G

G

A

A

K

S

I

V

G

A

N

N

P

P

D

D

K

A

T

I

V

V

F

V

A

D

I

I

E

D

G

G

D
|
D

G

G

S

S

F

F

V

I

M

M

N

N

M

V

Q

Q

D

E

V

L

I

A

T

T

A

I

V

R

Q

V

Y

E

R

N

I

L

P

P

V

V

K

K

I

V

A

L

I

L

N

N

N
|
N

E

Q

F
x
H

L
|
L

G

G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

Q

D

L

R

F

F

Y

Y

D

K

S

A

R

N

Y

R

S

A

S

H

V

T

C

F

L

L

A

G

-

D

-

P

-

A

-

Q

-

E

-

D

-

E

V

I

H

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

S

A

M

C

G

G

A

I

V

P

G

A

L

A

R

R

A

V

T

T

K

K

P

K

E

A

E

D

V

L

R

R

P

E

V

A

L

I

E

Q

K

-

A

T

M

M

K

L

I

D

N

T

D

P

R

G

P

P

V

Y

I

L

M

L

D

D

F

V

V

I

V

C

D

P

R
x
H

E

Q

E

E

N

H

V

V

L

L

P

P

M

M

V

I

P

P

A
x
S

G

G

K

G

S

T

Y

F

R

N

E

D

M

V

I

I

active site: Y33 (≠ L25), G35 (= G27), G36 (= G28), A37 (= A29), S38 (≠ I30), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), M266 (= M261), V293 (= V288), V400 (= V395), G426 (= G421), M428 (= M423), D453 (= D448), N480 (= N475), H482 (≠ F477), L483 (= L478), M485 (= M480), V486 (= V481), W489 (= W484), H558 (≠ R547)
binding 2-[[[[(4-chloro-6-methoxy-2-pyrimidinyl)amino]carbonyl]amino]sulfonyl]benzoic acid ethyl ester: M266 (= M261), D291 (= D286), R292 (= R287), M485 (= M480), W489 (= W484), S568 (≠ A557)
binding flavin-adenine dinucleotide: R161 (= R152), G223 (= G216), G224 (= G217), T246 (= T241), L247 (≠ N242), M248 (= M243), L264 (= L259), M266 (= M261), H267 (= H262), G286 (= G281), V287 (≠ A282), R288 (= R283), D290 (= D285), V293 (= V288), D310 (= D305), I311 (= I306), D329 (= D324), V330 (= V325), Q404 (= Q399), M405 (= M400), G423 (= G418), G424 (= G419)
binding magnesium ion: D453 (= D448), N480 (= N475), H482 (≠ F477)

7tzzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase p197t mutant in complex with bispyribac-sodium (see paper)
48% identity, 97% coverage: 4:565/581 of query aligns to 12:576/582 of 7tzzA

query
sites
7tzzA

K

R

R

K

G

G

A

A

D

D

I

I

V

L

I

V

D

E

V

A

L

L

L

E

E

R

E

Q

G

G

V

V

D

E

T

T

V

V

F

F

G

A

L

Y

P

P

G

G

A
|
A

I

S

M

M

E

E

V

I

Y

H

D

Q

A

A

L

L

F

T

-

R

D

S

A

S

P

S

L

I

R

R

N

N

I

V

L

L

A

P

R

R

H

H

E

E

Q

Q

A

G

A

V

H

F

M

A

A

A

D

E

G

G

Y

Y

A

A

R

R

A

S

T

S

G

G

K

K

V

P

G

G

V

I

V

C

L

I

A

A

T
|
T

S
|
S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

G

G

I

L

A

A

T

D

A

A

Y

L

M

L

D

D

S

S

V

V

P

P

L

L

V

V

A

A

I

I

T

T

G

G

Q

Q

V

V

P

T

T

R

S

R

Y

M

I

I

G

G

T

T

D

D

A

A

F

F

Q
|
Q

E

E

A

T

D

P

V

I

V

V

G

E

I

V

T

T

R

R

P

S

I

I

T

T

K

K

H

H

N

N

F

Y

L

L

V

V

T

M

D

D

I

V

K

E

D

D

L

I

A

P

L

R

I

I

L

I

R

E

E

E

A

A

F

F

Y

F

L

L

A

A

R

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

V

V

D

D

I

V

P

P

K

K

D

D

I

I

T

Q

Q

Q

E

-

Y

-

D

-

Y

L

K

A

M

I

P

P

T

N

E

W

K

E

D

Q

V

A

L

M

D

-

A

R

L

L

P

P

G

G

Y

Y

K

M

P

S

H

R

Y

M

E

P

G

K

N

P

P

P

-

E

-

D

F

S

Q

H

I

L

K

E

K

Q

A

I

A

V

E

R

L

L

I

I

R

S

K

E

A

S

K

K

R

K

P

P

V

V

L

L

Y

Y

V

V

G
|
G

T

C

V

L

I

N

S

S

G

-

A

-

S

S

E

D

E

E

L

L

R

G

E

R

L

F

A

V

E

E

L

L

T

T

R

G

I

I

P

P

V

V

T

A

T

S

T
|
T

N
x
L

M
|
M

A

G

K

L

G

G

A

S

F

Y

D

P

E

C

T

D

H

D

P

E

L

L

A

S

L

L

H

H

M
|
M

L
|
L

G

G

M
|
M

H
|
H

G

G

T

T

Y

V

Y

Y

A

A

N

N

M

Y

A

A

V

V

Y

E

H

H

S

S

D

D

L

L

I

L

A

A

V

F

G
|
G

A

V

R
|
R

F

F

D

D

R
|
R

V
|
V

T

T

G

G

K

K

I

L

Q

E

E

A

F

F

A

A

P

S

E

R

A

A

K

K

I

I

I

V

H

H

I

I

D
|
D

I
|
I

D

D

P

S

A

A

S

E

I

I

S

G

K

K

N

N

I

K

T

T

V

P

D

H

V

V

P

S

I

V

V

C

G

G

D
|
D

V
|
V

K

K

V

L

V

A

L

L

G

Q

K

G

L

M

L

N

K

K

E

V

L

L

K

E

K

N

K

R

P

A

I

E

E

E

W

L

V

K

K

L

A

D

R

F

E

G

S

V

W

W

L

R

K

N

Q

E

I

L

E

N

K

V

W

Q

K

K

E

Q

K

K

H

F

P

P

L

L

T

S

Y

F

D

K

R

T

T

F

S

G

K

E

I

A

I

I

K

P

P

P

Q

Q

Y
|
Y

V

A

I

I

E

K

E

V

I

L

Y

D

K

E

I

L

T

T

D

D

G

G

E

K

A

A

L

I

I

I

T

S

A

T

G

G

V
|
V

G
|
G

Q
|
Q

H
|
H

Q

Q

M
|
M

W

W

A

A

M

Q

F

F

Y

Y

K

N

Y

Y

R

K

Y

K

P

P

R

R

Q

Q

F

W

L

L

N

S

S

S

G
|
G

G

G

L

L

G
|
G

T

A

M
|
M

G

G

Y

F

G

G

F

L

P

P

A

A

V

I

G

G

A

A

K

S

I

V

G

A

N

N

P

P

D

D

K

A

T

I

V

V

F

V

A

D

I

I

E

D

G
|
G

D
|
D

G
|
G

S
|
S

F

F

V

I

M
|
M

N

N

M

V

Q
|
Q

D

E

V

L

I

A

T

T

A

I

V

R

Q

V

Y

E

R

N

I

L

P

P

V

V

K

K

I

V

A

L

I

L

N

N

N
|
N

E

Q

F
x
H

L
|
L

G
|
G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

Q

D

L

R

F

F

Y

Y

D

K

S

A

R

N

Y

R

S

A

S

H

V

T

C

F

L

L

A

G

-

D

-

P

-

A

-

Q

-

E

-

D

-

E

V

I

H

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

S

A

M

C

G

G

A

I

V

P

G

A

L

A

R

R

A

V

T

T

K

K

P
x
K

E

A

E

D

V

L

R

R

P

E

V

A

L

I

E

Q

K

-

A

T

M

M

K

L

I

D

N

T

D

P

R

G

P

P

V

Y

I

L

M

L

D

D

F

V

V

I

V

C

D

P

R

H

E

Q

E

E

N

H

V

V

L

L

P

P

M

M

V

I

P

P

A
x
S

G

G

K

G

S

T

Y

F

R

N

E

D

M

V

I

I

binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: M266 (= M261), R292 (= R287), W489 (= W484), S568 (≠ A557)
binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V395), G401 (= G396), Q402 (= Q397), H403 (= H398), G426 (= G421), M428 (= M423), G452 (= G447), D453 (= D448), G454 (= G449), S455 (= S450), L483 (= L478), G484 (= G479), M485 (= M480), V486 (= V481)
binding flavin-adenine dinucleotide: R161 (= R152), G222 (= G215), G223 (= G216), G224 (= G217), T246 (= T241), L247 (≠ N242), M248 (= M243), M263 (= M258), L264 (= L259), M266 (= M261), H267 (= H262), G286 (= G281), R288 (= R283), V293 (= V288), D310 (= D305), I311 (= I306), D329 (= D324), V330 (= V325), M405 (= M400), G423 (= G418)
binding magnesium ion: A37 (= A29), T82 (= T73), S83 (= S74), Q122 (= Q113), Y381 (= Y376), D453 (= D448), M458 (= M453), Q461 (= Q456), N480 (= N475), H482 (≠ F477), K533 (≠ P521)

1t9cA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, sulfometuron methyl (see paper)
48% identity, 96% coverage: 6:565/581 of query aligns to 10:574/596 of 1t9cA

query
sites
1t9cA

G

G

A

G

D

Q

I

I

V

F

I

N

D

E

V

M

L

M

L

S

E

R

E

Q

G

N

V

V

D

D

T

T

V

V

F

F

G

G

L
x
Y

P

P

G
|
G

A
|
A

I
|
I

M

L

E

P

V

V

Y

Y

D

D

A

A

L

I

F

H

D

N

A

S

P

D

L

K

R

F

N

N

-

F

I

V

L

L

A

P

R

K

H

H

E
|
E

Q

Q

A

G

A

A

A

G

H

H

M

M

A

A

D

E

G

G

Y

Y

A

A

R

R

A

A

T

S

G

G

K

K

V

P

G

G

V

V

L

L

A

V

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

V

V

V

T

T

G

P

I

M

A

A

T

D

A

A

Y

F

M

A

D

D

S

G

V

I

P

P

L

M

V

V

A

V

I

F

T

T

G

G

Q

Q

V
|
V

P
|
P

T

T

S

S

Y

A

I

I

G

G

T

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

A

A

D

D

V

V

G

G

I

I

T

S

R

R

P

S

I

C

T

T

K

K

H

W

N

N

F

V

L

M

V

V

T

K

D

S

I

V

K

E

D

E

L

L

A

P

L

L

I

R

L

I

R

N

E

E

A

A

F

F

Y

E

L

I

A

A

R

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

V

V

D

D

I

L

P

P

K
|
K

D

D

I

V

T

T

Q

A

E

I

Y

L

D

R

Y

N

K

P

M

I

P

P

T

T

E

K

K

T

D

-

V

-

L

-

D

-

A

T

L

L

P

P

G

S

Y

S

K

R

P

A

H

Q

Y

D

E

E

G

F

N

V

P

M

F

Q

Q

S

I

I

K

N

K

K

A

A

E

D

L

L

I

I

R

N

K

L

A

A

K

K

R

K

P

P

V

V

L

L

Y

Y

V

V

G
|
G

G
x
A

G
|
G

T

I

V

L

I
x
N

-

H

S

A

G

D

A

G

S

P

E
x
R

E

L

L

L

R

K

E

E

L

L

A

S

E

D

L

R

T

A

R

Q

I

I

P

P

V

V

T

T

T
|
T

N
x
L

M
x
Q

A

G

K

L

G

G

A

S

F

F

D

D

E

Q

T

E

H

D

P

P

L

K

A

S

L

L

H

D

M

M

L
|
L

G

G

M
|
M

H
|
H

G

G

T

C

Y

A

Y

T

A

A

N

N

M

L

A

A

V

V

Y

Q

H

N

S

A

D

D

L

L

L

I

I

I

A

A

V

V

G
|
G

A
|
A

R
|
R

F

F

D
|
D

R
|
R

V
|
V

T

T

G

G

K

N

I

I

Q

S

E

K

F

F

A

A

P

P

-

E

-

A

-

R

-

A

-

E

-

G

E

R

A

G

K

G

I

I

H

H

I

F

D
x
E

I
x
V

D

S

P

P

A

K

S
x
N

I

I

S

N

K

K

N

V

I

V

T

Q

V

T

D

Q

V

I

P

A

I

V

V

E

G
|
G

D
|
D

V
x
A

K

T

V

T

V

N

L

L

G

G

K

K

L

M

K

S

E

K

L

I

K

F

K

P

K

-

P

-

I

-

E

-

W

-

V

V

K

K

A

E

R

R

E

S

S

E

W

W

L

F

K

A

Q

Q

I

I

E

N

K

K

W

W

K

K

E

K

K

E

H

Y

P

P

L

Y

T

A

Y

Y

-

M

-

E

D

E

R

T

T

P

S

G

K

S

I

K

I

I

K

K

P

P

Q

Q

Y

T

V

V

I

I

E

K

I

L

Y

S

K

K

I

V

-

A

-

N

-

D

T

T

D

G

G

R

E

H

A

V

L

I

I

V

T

T

A

T

G

G

V
|
V

G

G

Q

Q

H

H

Q

Q

M
|
M

W

W

A

A

M

Q

F

H

Y

W

K

T

Y

W

R

R

Y

N

P

P

R

H

Q

T

F

F

L

I

N

T

S

S

G
|
G

L
|
L

G
|
G

T

T

M
|
M

G

G

Y

Y

G

G

F

L

P

P

A

A

V

I

G

G

A

A

K

Q

I

V

G

A

N

K

P

P

D

E

K

S

T

L

V

V

F

I

A

D

I

I

E

D

G

G

D
|
D

G

A

S

S

F

F

V

N

M

M

N

T

M

L

Q

T

D

E

V

L

I

S

T

S

A

A

V

V

Q

Q

Y

A

R

G

I

T

P

P

V

V

K

K

I

I

A

L

I

I

I

L

N

N

N
|
N

E

E

F
x
E

L
x
Q

G

G

M
|
M

V
|
V

R

T

Q

Q

W
|
W

Q

Q

Q

S

L

L

F

F

Y

Y

D

E

S

H

R

R

Y

Y

S

S

S

H

V

T

C

H

L

-

A

Q

V

L

H

N

P

P

D

D

F

F

V

I

K

K

L
|
L

A

A

E

E

S

A

M

M

G
|
G

A
x
L

V

K

G

G

L

L

R

R

A

V

T

K

K

K

P

Q

E

E

V

L

R

D

P

A

V

K

L

L

E

K

K

E

A

F

M

V

K

S

I

T

N

K

D

G

R

-

P

P

V

V

I

L

M

L

D

E

F

V

V

E

V

V

D

D

R
x
K

E

K

E

V

N

P

V

V

L

L

P

P

M

M

V

V

P

A

A

G

G

G

K

S

S

G

Y

L

R

D

E

E

M

F

I

I

active site: Y29 (≠ L25), G31 (= G27), G32 (= G28), A33 (= A29), I34 (= I30), E55 (= E50), T78 (= T73), F117 (= F112), Q118 (= Q113), E119 (= E114), K167 (= K162), R227 (≠ E225), M263 (= M261), V290 (= V288), V406 (= V395), L431 (= L420), G432 (= G421), M434 (= M423), D459 (= D448), N486 (= N475), E488 (≠ F477), Q489 (≠ L478), M491 (= M480), V492 (= V481), W495 (= W484), L517 (= L507), G522 (= G512), L523 (≠ A513), K556 (≠ R547)
binding methyl 2-[({[(4,6-dimethylpyrimidin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: G32 (= G28), V107 (= V102), P108 (= P103), F117 (= F112), K167 (= K162), D288 (= D286), R289 (= R287), W495 (= W484)
binding flavin-adenine dinucleotide: R157 (= R152), G216 (= G215), A217 (≠ G216), G218 (= G217), N221 (≠ I220), T243 (= T241), L244 (≠ N242), Q245 (≠ M243), L261 (= L259), M263 (= M261), H264 (= H262), G283 (= G281), A284 (= A282), R285 (= R283), D287 (= D285), R289 (= R287), V290 (= V288), E316 (≠ D305), V317 (≠ I306), N321 (≠ S310), G334 (= G323), D335 (= D324), A336 (≠ V325), M411 (= M400), G429 (= G418), G430 (= G419)
binding magnesium ion: D459 (= D448), N486 (= N475), E488 (≠ F477)

1t9dA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, metsulfuron methyl (see paper)
48% identity, 96% coverage: 6:565/581 of query aligns to 10:574/596 of 1t9dA

query
sites
1t9dA

G

G

A

G

D

Q

I

I

V

F

I

N

D

E

V

M

L

M

L

S

E

R

E

Q

G

N

V

V

D

D

T

T

V

V

F

F

G

G

L
x
Y

P

P

G
|
G

A
|
A

I
|
I

M

L

E

P

V

V

Y

Y

D

D

A

A

L

I

F

H

D

N

A

S

P

D

L

K

R

F

N

N

-

F

I

V

L

L

A

P

R

K

H

H

E
|
E

Q

Q

A

G

A

A

A

G

H

H

M

M

A

A

D

E

G

G

Y

Y

A

A

R

R

A

A

T

S

G

G

K

K

V

P

G

G

V

V

L

L

A

V

T
|
T

S

S

G

G

P
|
P

G

G

A

A

T

T

N

N

L

V

V

V

T

T

G

P

I

M

A

A

T

D

A

A

Y

F

M

A

D

D

S

G

V

I

P

P

L

M

V

V

A

V

I

F

T

T

G

G

Q

Q

V
|
V

P
|
P

T

T

S

S

Y

A

I

I

G

G

T

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

A

A

D

D

V

V

G

G

I

I

T

S

R

R

P

S

I

C

T

T

K

K

H

W

N

N

F

V

L

M

V

V

T

K

D

S

I

V

K

E

D

E

L

L

A

P

L

L

I

R

L

I

R

N

E

E

A

A

F

F

Y

E

L

I

A

A

R

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

V

V

D

D

I

L

P

P

K
|
K

D

D

I

V

T

T

Q

A

E

I

Y

L

D

R

Y

N

K

P

M

I

P

P

T

T

E

K

K

T

D

-

V

-

L

-

D

-

A

T

L

L

P

P

G

T

Y

S

K

R

P

A

H

Q

Y

D

E

E

G

F

N

V

P

M

F

Q

Q

S

I

I

K

N

K

K

A

A

E

D

L

L

I

I

R

N

K

L

A

A

K

K

R

K

P

P

V

V

L

L

Y

Y

V

V

G
|
G

G
x
A

G
|
G

T

I

V

L

I
x
N

S

H

G

A

A

D

S

G

E

P

E
x
R

-

L

L

L

R

K

E

E

L

L

A

S

E

D

L

R

T

A

R

Q

I

I

P

P

V

V

T

T

T
|
T

N
x
L

M
x
Q

A

G

K

L

G

G

A

S

F

F

D

D

E

Q

T

E

H

D

P

P

L

K

A

S

L

L

H

D

M
|
M

L
|
L

G

G

M
|
M

H
|
H

G

G

T

C

Y

A

Y

T

A

A

N

N

M

L

A

A

V

V

Y

Q

H

N

S

A

D

D

L

L

L

I

I

I

A

A

V

V

G
|
G

A
|
A

R
|
R

F

F

D
|
D

R
|
R

V
|
V

T

T

G

G

K

N

I

I

Q

S

E

K

F

F

A

A

P

P

-

E

-

A

-

R

-

A

-

E

-

G

E

R

A

G

K

G

I

I

H

H

I

F

D
x
E

I
x
V

D

S

P

P

A

K

S
x
N

I

I

S

N

K

K

N

V

I

V

T

Q

V

T

D

Q

V

I

P

A

I

V

V

E

G
|
G

D
|
D

V
x
A

K

T

V

T

V

N

L

L

G

G

K

K

L

M

K

S

E

K

L

I

K

F

K

P

K

-

P

-

I

-

E

-

W

-

V

V

K

K

A

E

R

R

E

S

S

E

W

W

L

F

K

A

Q

Q

I

I

E

N

K

K

W

W

K

K

E

K

K

E

H

Y

P

P

L

Y

T

A

Y

Y

-

M

-

E

D

E

R

T

T

P

S

G

K

S

I

K

I

I

K

K

P

P

Q

Q

Y

T

V

V

I

I

E

K

I

L

Y

S

K

K

I

V

-

A

-

N

-

D

T

T

D

G

G

R

E

H

A

V

L

I

I

V

T

T

A

T

G

G

V
|
V

G

G

Q

Q

H

H

Q
|
Q

M
|
M

W

W

A

A

M

Q

F

H

Y

W

K

T

Y

W

R

R

Y

N

P

P

R

H

Q

T

F

F

L

I

N

T

S

S

G
|
G

L
|
L

G
|
G

T

T

M
|
M

G

G

Y

Y

G

G

F

L

P

P

A

A

V

I

G

G

A

A

K

Q

I

V

G

A

N

K

P

P

D

E

K

S

T

L

V

V

F

I

A

D

I

I

E

D

G

G

D
|
D

G

A

S

S

F

F

V

N

M
|
M

N

T

M

L

Q

T

D

E

V

L

I

S

T

S

A

A

V

V

Q

Q

Y

A

R

G

I

T

P

P

V

V

K

K

I

I

A

L

I

I

I

L

N

N

N
|
N

E

E

F
x
E

L
x
Q

G

G

M
|
M

V
|
V

R

T

Q

Q

W
|
W

Q

Q

Q

S

L

L

F

F

Y

Y

D

E

S

H

R

R

Y

Y

S

S

S

H

V

T

C

H

L

-

A

Q

V

L

H

N

P

P

D

D

F

F

V

I

K

K

L
|
L

A

A

E

E

S

A

M

M

G
|
G

A
x
L

V

K

G

G

L

L

R

R

A

V

T

K

K

K

P

Q

E

E

V

L

R

D

P

A

V

K

L

L

E

K

K

E

A

F

M

V

K

S

I

T

N

K

D

G

R

-

P

P

V

V

I

L

M

L

D

E

F

V

V

E

V

V

D

D

R
x
K

E

K

E

V

N

P

V

V

L

L

P

P

M

M

V

V

P

A

A

G

G

G

K

S

S

G

Y

L

R

D

E

E

M

F

I

I

active site: Y29 (≠ L25), G31 (= G27), G32 (= G28), A33 (= A29), I34 (= I30), E55 (= E50), T78 (= T73), F117 (= F112), Q118 (= Q113), E119 (= E114), K167 (= K162), R227 (≠ E226), M263 (= M261), V290 (= V288), V406 (= V395), L431 (= L420), G432 (= G421), M434 (= M423), D459 (= D448), N486 (= N475), E488 (≠ F477), Q489 (≠ L478), M491 (= M480), V492 (= V481), W495 (= W484), L517 (= L507), G522 (= G512), L523 (≠ A513), K556 (≠ R547)
binding methyl 2-[({[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: G32 (= G28), A33 (= A29), V107 (= V102), P108 (= P103), F117 (= F112), K167 (= K162), M263 (= M261), D288 (= D286), R289 (= R287), W495 (= W484)
binding flavin-adenine dinucleotide: R157 (= R152), G216 (= G215), A217 (≠ G216), G218 (= G217), N221 (≠ I220), T243 (= T241), L244 (≠ N242), Q245 (≠ M243), M260 (= M258), L261 (= L259), H264 (= H262), G283 (= G281), A284 (= A282), R285 (= R283), D287 (= D285), R289 (= R287), V290 (= V288), E316 (≠ D305), V317 (≠ I306), N321 (≠ S310), G334 (= G323), D335 (= D324), A336 (≠ V325), Q410 (= Q399), M411 (= M400), G429 (= G418), G430 (= G419)
binding magnesium ion: D459 (= D448), N486 (= N475), E488 (≠ F477)
binding 2,5-dimethyl-pyrimidin-4-ylamine: E55 (= E50), P81 (= P76), Q118 (= Q113), G432 (= G421), M434 (= M423), M464 (= M453)

Query Sequence

>WP_012676796.1 NCBI__GCF_000021565.1:WP_012676796.1
MPKKRGADIVIDVLLEEGVDTVFGLPGGAIMEVYDALFDAPLRNILARHEQAAAHMADGY
ARATGKVGVVLATSGPGATNLVTGIATAYMDSVPLVAITGQVPTSYIGTDAFQEADVVGI
TRPITKHNFLVTDIKDLALILREAFYLARTGRPGPVLVDIPKDITQQEYDYKMPTEKDVL
DALPGYKPHYEGNPFQIKKAAELIRKAKRPVLYVGGGTVISGASEELRELAELTRIPVTT
TNMAKGAFDETHPLALHMLGMHGTYYANMAVYHSDLLIAVGARFDDRVTGKIQEFAPEAK
IIHIDIDPASISKNITVDVPIVGDVKVVLGKLLKELKKKPIEWVKARESWLKQIEKWKEK
HPLTYDRTSKIIKPQYVIEEIYKITDGEALITAGVGQHQMWAAMFYKYRYPRQFLNSGGL
GTMGYGFPAAVGAKIGNPDKTVFAIEGDGSFVMNMQDVITAVQYRIPVKIAIINNEFLGM
VRQWQQLFYDSRYSSVCLAVHPDFVKLAESMGAVGLRATKPEEVRPVLEKAMKINDRPVI
MDFVVDREENVLPMVPAGKSYREMIVSPKQKGEAETMYLVG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory