SitesBLAST
Comparing WP_012676942.1 NCBI__GCF_000021565.1:WP_012676942.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
2zsjA Crystal structure of threonine synthase from aquifex aeolicus vf5
76% identity, 99% coverage: 5:356/356 of query aligns to 1:350/350 of 2zsjA
- active site: K61 (= K67), T85 (= T91), Q218 (= Q224), A222 (= A228), A240 (= A246), T317 (= T323)
- binding pyridoxal-5'-phosphate: F60 (= F66), K61 (= K67), N87 (= N93), V186 (= V192), G187 (= G193), N188 (= N194), A189 (= A195), G190 (= G196), N191 (= N197), A240 (= A246), T317 (= T323), G318 (= G324)
6cgqB Threonine synthase from bacillus subtilis atcc 6633 with plp and plp- ala (see paper)
61% identity, 98% coverage: 6:354/356 of query aligns to 1:345/345 of 6cgqB
- active site: K58 (= K67), T82 (= T91), E214 (≠ Q224), S218 (≠ A228), A236 (= A246), T313 (= T323)
- binding (E)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-alanine: K58 (= K67), S81 (= S90), T82 (= T91), N84 (= N93), T85 (= T94), V183 (= V192), G184 (= G193), N185 (= N194), A186 (= A195), N188 (= N197), A236 (= A246), I237 (= I247), E283 (= E293), T313 (= T323)
- binding phosphate ion: K58 (= K67), T85 (= T94), N151 (= N160), S152 (= S161), R157 (= R166), N185 (= N194)
6nmxA Threonine synthase from bacillus subtilis atcc 6633 with plp and appa (see paper)
61% identity, 98% coverage: 6:354/356 of query aligns to 3:347/350 of 6nmxA
- active site: K60 (= K67), T84 (= T91), E216 (≠ Q224), S220 (≠ A228), A238 (= A246), T315 (= T323)
- binding (2E,3Z)-2-{[(Z)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4(1H)-ylidene}methyl]imino}-5-phosphonopent-3-enoic acid: K60 (= K67), S83 (= S90), T84 (= T91), N86 (= N93), T87 (= T94), F133 (= F140), N153 (= N160), S154 (= S161), R159 (= R166), V185 (= V192), G186 (= G193), N187 (= N194), A188 (= A195), G189 (= G196), N190 (= N197), A238 (= A246), I239 (= I247), E285 (= E293), T315 (= T323)
1uimA Crystal structure of threonine synthase from thermus thermophilus hb8, orthorhombic crystal form (see paper)
59% identity, 98% coverage: 9:356/356 of query aligns to 5:350/350 of 1uimA
- active site: K61 (= K67), T85 (= T91), P212 (= P218), G216 (= G222), Q218 (= Q224), A240 (= A246), T317 (= T323)
- binding pyridoxal-5'-phosphate: F60 (= F66), K61 (= K67), N87 (= N93), G187 (= G193), N188 (= N194), A189 (= A195), G190 (= G196), N191 (= N197), A240 (= A246), E287 (= E293), T317 (= T323), G318 (= G324)
3aeyA Apo form of threonine synthase from thermus thermophilus hb8 (see paper)
59% identity, 98% coverage: 9:356/356 of query aligns to 4:349/350 of 3aeyA
- active site: K60 (= K67), T84 (= T91), P211 (= P218), G215 (= G222), Q217 (= Q224), A239 (= A246), T316 (= T323)
- binding sulfate ion: K60 (= K67), K60 (= K67), G85 (= G92), N86 (= N93), T87 (= T94), T87 (= T94), S154 (= S161), R159 (= R166), N187 (= N194), R228 (≠ F235), V230 (≠ I237), E231 (≠ K238), R232 (≠ N239), A239 (= A246)
3aexA Catalytic intermediate analogue of threonine synthase from thermus thermophilus hb8 (see paper)
59% identity, 98% coverage: 9:356/356 of query aligns to 5:350/351 of 3aexA
- active site: K61 (= K67), T85 (= T91), P212 (= P218), G216 (= G222), Q218 (= Q224), A240 (= A246), T317 (= T323)
- binding (3E)-4-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}-2-oxobut-3-enoic acid: K61 (= K67), S84 (= S90), T85 (= T91), N87 (= N93), T88 (= T94), V186 (= V192), G187 (= G193), N188 (= N194), A189 (= A195), G190 (= G196), N191 (= N197), A240 (= A246), I241 (= I247), E287 (= E293), T317 (= T323)
- binding phosphate ion: K61 (= K67), T88 (= T94), N154 (= N160), S155 (= S161), R160 (= R166), N188 (= N194)
1v7cA Crystal structure of threonine synthase from thermus thermophilus hb8 in complex with a substrate analogue (see paper)
59% identity, 98% coverage: 9:356/356 of query aligns to 5:350/351 of 1v7cA
- active site: K61 (= K67), T85 (= T91), P212 (= P218), G216 (= G222), Q218 (= Q224), A240 (= A246), T317 (= T323)
- binding (2e)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]-5-phosphonopent-2-enoic acid: K61 (= K67), S84 (= S90), T85 (= T91), N87 (= N93), T88 (= T94), F134 (= F140), N154 (= N160), S155 (= S161), R160 (= R166), V186 (= V192), G187 (= G193), N188 (= N194), A189 (= A195), G190 (= G196), N191 (= N197), A240 (= A246), I241 (= I247), E287 (= E293), T317 (= T323)
A0R220 Threonine synthase; TS; EC 4.2.3.1 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
59% identity, 99% coverage: 6:356/356 of query aligns to 12:359/360 of A0R220
- K151 (≠ E149) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
6cgqA Threonine synthase from bacillus subtilis atcc 6633 with plp and plp- ala (see paper)
60% identity, 97% coverage: 8:354/356 of query aligns to 1:337/339 of 6cgqA
- active site: K56 (= K67), T80 (= T91), E206 (≠ Q224), S210 (≠ A228), A228 (= A246), T305 (= T323)
- binding pyridoxal-5'-phosphate: F55 (= F66), K56 (= K67), N82 (= N93), V175 (= V192), G176 (= G193), N177 (= N194), A178 (= A195), G179 (= G196), N180 (= N197), A228 (= A246), E275 (= E293), T305 (= T323), G306 (= G324)
P9WG59 Threonine synthase; TS; EC 4.2.3.1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
63% identity, 93% coverage: 6:335/356 of query aligns to 12:338/360 of P9WG59
- K69 (= K67) modified: N6-(pyridoxal phosphate)lysine
- N95 (= N93) binding pyridoxal 5'-phosphate
- K151 (≠ E149) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
- GNAGN 196:200 (= GNAGN 193:197) binding pyridoxal 5'-phosphate
- T326 (= T323) binding pyridoxal 5'-phosphate
2d1fA Structure of mycobacterium tuberculosis threonine synthase (see paper)
63% identity, 93% coverage: 6:335/356 of query aligns to 3:329/349 of 2d1fA
- active site: K60 (= K67), T84 (= T91), D209 (≠ T215), R213 (= R219), L215 (≠ I221), A240 (= A246), T317 (= T323)
- binding pyridoxal-5'-phosphate: F59 (= F66), K60 (= K67), N86 (= N93), V186 (= V192), G187 (= G193), N188 (= N194), A189 (= A195), G190 (= G196), N191 (= N197), A240 (= A246), T317 (= T323)
Q9S7B5 Threonine synthase 1, chloroplastic; Protein METHIONINE OVER-ACCUMULATOR 2; EC 4.2.3.1 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
33% identity, 98% coverage: 8:355/356 of query aligns to 146:520/526 of Q9S7B5
- N172 (≠ P34) binding S-adenosyl-L-methionine
- L173 (= L35) binding S-adenosyl-L-methionine
- K181 (= K43) binding in monomer B; binding in monomer A
- N187 (≠ K49) binding in monomer B
- L205 (≠ R69) mutation to R: In mto2-1; causes a strong decrease in the concentration of soluble threonine and over-accumulation of methionine.
2c2bA Crystallographic structure of arabidopsis thaliana threonine synthase complexed with pyridoxal phosphate and s-adenosylmethionine (see paper)
33% identity, 97% coverage: 8:354/356 of query aligns to 71:440/444 of 2c2bA
- binding pyridoxal-5'-phosphate: F127 (= F66), K128 (= K67), D159 (≠ N93), G259 (≠ V192), G260 (= G193), N261 (= N194), L262 (≠ A195), G263 (= G196), N264 (= N197), A321 (= A246), H369 (≠ A295), T397 (= T323)
- binding s-adenosylmethionine: S90 (≠ T27), F92 (≠ Y29), N97 (≠ P34), L98 (= L35), W100 (≠ D37), W115 (≠ Y54), W115 (≠ Y54), Q246 (≠ A180), F247 (≠ L181)
Sites not aligning to the query:
2c2gA Crystal structure of threonine synthase from arabidopsis thaliana in complex with its cofactor pyridoxal phosphate (see paper)
32% identity, 98% coverage: 8:355/356 of query aligns to 89:447/448 of 2c2gA
7mfjBBB Beta-cyanoalanine synthase (see paper)
29% identity, 86% coverage: 23:328/356 of query aligns to 14:318/319 of 7mfjBBB
8y1jA Structure of the pyridoxal 5'-phosphate-dependent (plp) threonine deaminase ilva1 from pseudomonas aeruginosa pao1 (see paper)
29% identity, 82% coverage: 32:324/356 of query aligns to 19:305/502 of 8y1jA
Sites not aligning to the query:
P16703 Cysteine synthase B; CSase B; O-acetylserine (thiol)-lyase B; OAS-TL B; O-acetylserine sulfhydrylase B; EC 2.5.1.47 from Escherichia coli (strain K12) (see paper)
25% identity, 77% coverage: 31:305/356 of query aligns to 9:265/303 of P16703
- N71 (= N93) binding pyridoxal 5'-phosphate
- S255 (≠ A295) binding pyridoxal 5'-phosphate
2bhtA Crystal structure of o-acetylserine sulfhydrylase b (see paper)
25% identity, 77% coverage: 31:305/356 of query aligns to 9:265/294 of 2bhtA
- active site: K41 (= K67), S69 (≠ T91), Q199 (= Q224), G203 (vs. gap), S255 (≠ A295)
- binding pyridoxal-5'-phosphate: K41 (= K67), N71 (= N93), M173 (≠ V192), G174 (= G193), T175 (≠ N194), T176 (≠ A195), T178 (≠ N197), G208 (≠ I231), S255 (≠ A295)
Sites not aligning to the query:
2gn2A Crystal structure of tetrameric biodegradative threonine deaminase (tdcb) from salmonella typhimurium in complex with cmp at 2.5a resolution (hexagonal form) (see paper)
33% identity, 38% coverage: 52:186/356 of query aligns to 41:176/326 of 2gn2A
Sites not aligning to the query:
2zr8A Crystal structure of modified serine racemase complexed with serine (see paper)
25% identity, 77% coverage: 29:301/356 of query aligns to 19:285/319 of 2zr8A
- active site: K53 (= K67), S78 (≠ T91), E204 (vs. gap), G208 (= G212), D210 (≠ S214), G232 (≠ A246)
- binding magnesium ion: E204 (vs. gap), G208 (= G212), D210 (≠ S214)
- binding n-(5'-phosphopyridoxyl)-d-alanine: F52 (= F66), K53 (= K67), S77 (= S90), S78 (≠ T91), N80 (= N93), H81 (≠ T94), P147 (≠ S161), G179 (= G193), G180 (≠ N194), G181 (≠ A195), G182 (= G196), G232 (≠ A246), E277 (= E293), T279 (≠ A295)
- binding serine: S78 (≠ T91), R129 (≠ A143), D231 (≠ T245), G232 (≠ A246), A233 (≠ I247), Q234 (≠ K248), T235 (≠ I249)
Sites not aligning to the query:
Query Sequence
>WP_012676942.1 NCBI__GCF_000021565.1:WP_012676942.1
MDNCKWRGIITAYREFLPVTDKTPVVTLYEGNTPLIDAPNLSKKIAPDKDLKIYLKYEGL
NPTGSFKDRGMTMAISKAKESGKTAVICASTGNTSASAAAYAARAGMDAYVILPKGAVAL
GKLSQAMVYGAKIIALMGNFDDALSIVREIGEKYPVEVVNSVNPYRIEGQKTASFEIIDA
LGDAPDYHFIPVGNAGNITAYWKGYKEYKEAGRSTKLPRMIGWQAEGAAPIVKGFPIKNP
QTIATAIKIGNPYSWQPALQAAKESNGFIDAVSDEEILEAYRLVASTEGVFCEPASAASV
AGVIKAYRKGLFKGGETVVCTLTGNGLKDPDTVIKASEKPVEMPPDINEIARYLGL
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory