SitesBLAST
Comparing WP_012706368.1 NCBI__GCF_000018545.1:WP_012706368.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
5wkcA Saccharomyces cerevisiae acetohydroxyacid synthase in complex with the herbicide penoxsulam (see paper)
29% identity, 96% coverage: 1:539/561 of query aligns to 1:550/591 of 5wkcA
- active site: Y29 (≠ V29), G31 (= G31), G32 (≠ E32), A33 (≠ S33), I34 (≠ Y34), E55 (= E56), T78 (= T79), F117 (= F118), Q118 (= Q119), E119 (= E120), K167 (≠ E168), R222 (≠ A223), M258 (≠ L259), V285 (≠ T286), V401 (≠ A391), L426 (≠ P413), G427 (= G416), M429 (= M418), D454 (= D443), N481 (= N470), E483 (≠ M472), Q484 (≠ Y473), M486 (≠ T475), V487 (≠ I476), W490 (≠ H479), L512 (= L501), G517 (= G506), L518 (= L507)
- binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V401 (≠ A391), G402 (= G392), Q403 (≠ N393), H404 (≠ Y394), G427 (= G416), M429 (= M418), G453 (= G442), D454 (= D443), A455 (≠ G444), S456 (≠ C445), M459 (= M448), N481 (= N470), E483 (≠ M472), Q484 (≠ Y473), G485 (= G474), M486 (≠ T475), V487 (≠ I476)
- binding ethaneperoxoic acid: G32 (≠ E32), Q118 (= Q119)
- binding flavin-adenine dinucleotide: R157 (= R158), G211 (= G213), A212 (≠ G214), G213 (≠ A215), N216 (≠ T218), T238 (≠ S239), L239 (≠ F240), Q240 (≠ R241), L256 (= L257), M258 (≠ L259), G278 (= G279), A279 (= A280), R280 (= R281), R284 (≠ M285), V285 (≠ T286), E311 (≠ H306), V312 (≠ A307), N316 (≠ E311), D330 (≠ S325), A331 (= A326), M406 (≠ A396), G424 (≠ L411)
- binding magnesium ion: D454 (= D443), N481 (= N470), E483 (≠ M472)
- binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: G32 (≠ E32), A33 (≠ S33), V107 (= V108), F117 (= F118), K167 (≠ E168), M258 (≠ L259), R284 (≠ M285), M486 (≠ T475), W490 (≠ H479)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: P30 (= P30), E55 (= E56)
Sites not aligning to the query:
1n0hA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorimuron ethyl (see paper)
29% identity, 96% coverage: 1:539/561 of query aligns to 3:558/599 of 1n0hA
- active site: Y31 (≠ V29), G33 (= G31), G34 (≠ E32), A35 (≠ S33), I36 (≠ Y34), E57 (= E56), T80 (= T79), F119 (= F118), Q120 (= Q119), E121 (= E120), K169 (≠ E168), R230 (≠ A223), M266 (≠ L259), V293 (≠ T286), V409 (≠ A391), L434 (≠ P413), G435 (= G416), M437 (= M418), D462 (= D443), N489 (= N470), E491 (≠ M472), Q492 (≠ Y473), M494 (≠ T475), V495 (≠ I476), W498 (≠ H479), L520 (= L501), G525 (= G506), L526 (= L507)
- binding 4-{[(4'-amino-2'-methylpyrimidin-5'-yl)methyl]amino}pent-3-enyl diphosphate: V409 (≠ A391), G410 (= G392), Q411 (≠ N393), H412 (≠ Y394), G435 (= G416), M437 (= M418), G461 (= G442), D462 (= D443), A463 (≠ G444), S464 (≠ C445), M467 (= M448), N489 (= N470), E491 (≠ M472), Q492 (≠ Y473), G493 (= G474), V495 (≠ I476)
- binding 2-[[[[(4-chloro-6-methoxy-2-pyrimidinyl)amino]carbonyl]amino]sulfonyl]benzoic acid ethyl ester: G34 (≠ E32), A35 (≠ S33), V109 (= V108), P110 (≠ A109), F119 (= F118), K169 (≠ E168), M266 (≠ L259), D291 (≠ E284), R292 (≠ M285), V495 (≠ I476), W498 (≠ H479)
- binding flavin-adenine dinucleotide: R159 (= R158), G219 (= G213), A220 (≠ G214), G221 (≠ A215), N224 (≠ T218), T246 (≠ S239), L247 (≠ F240), Q248 (≠ R241), L264 (= L257), G265 (= G258), M266 (≠ L259), H267 (≠ A260), G286 (= G279), A287 (= A280), R288 (= R281), D290 (≠ G283), R292 (≠ M285), V293 (≠ T286), E319 (≠ H306), V320 (≠ A307), N324 (≠ E311), G337 (≠ A324), D338 (≠ S325), A339 (= A326), M414 (≠ A396), G432 (≠ L411), G433 (= G412)
- binding magnesium ion: D462 (= D443), N489 (= N470), E491 (≠ M472)
- binding thiamine diphosphate: Y31 (≠ V29), E57 (= E56), P83 (= P82)
Sites not aligning to the query:
1t9cA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, sulfometuron methyl (see paper)
29% identity, 96% coverage: 1:539/561 of query aligns to 1:555/596 of 1t9cA
- active site: Y29 (≠ V29), G31 (= G31), G32 (≠ E32), A33 (≠ S33), I34 (≠ Y34), E55 (= E56), T78 (= T79), F117 (= F118), Q118 (= Q119), E119 (= E120), K167 (≠ E168), R227 (≠ A223), M263 (≠ L259), V290 (≠ T286), V406 (≠ A391), L431 (≠ P413), G432 (= G416), M434 (= M418), D459 (= D443), N486 (= N470), E488 (≠ M472), Q489 (≠ Y473), M491 (≠ T475), V492 (≠ I476), W495 (≠ H479), L517 (= L501), G522 (= G506), L523 (= L507)
- binding methyl 2-[({[(4,6-dimethylpyrimidin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: G32 (≠ E32), V107 (= V108), P108 (≠ A109), F117 (= F118), K167 (≠ E168), D288 (≠ E284), R289 (≠ M285), W495 (≠ H479)
- binding flavin-adenine dinucleotide: R157 (= R158), G216 (= G213), A217 (≠ G214), G218 (≠ A215), N221 (≠ T218), T243 (≠ S239), L244 (≠ F240), Q245 (≠ R241), L261 (= L257), M263 (≠ L259), H264 (≠ A260), G283 (= G279), A284 (= A280), R285 (= R281), D287 (≠ G283), R289 (≠ M285), V290 (≠ T286), E316 (≠ H306), V317 (≠ A307), N321 (≠ E311), G334 (≠ A324), D335 (≠ S325), A336 (= A326), M411 (≠ A396), G429 (≠ L411), G430 (= G412)
- binding magnesium ion: D459 (= D443), N486 (= N470), E488 (≠ M472)
Sites not aligning to the query:
1t9aA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, tribenuron methyl (see paper)
29% identity, 96% coverage: 1:539/561 of query aligns to 2:556/597 of 1t9aA
- active site: Y30 (≠ V29), G32 (= G31), G33 (≠ E32), A34 (≠ S33), I35 (≠ Y34), E56 (= E56), T79 (= T79), F118 (= F118), Q119 (= Q119), E120 (= E120), K168 (≠ E168), R228 (≠ A223), M264 (≠ L259), V291 (≠ T286), V407 (≠ A391), L432 (≠ P413), G433 (= G416), M435 (= M418), D460 (= D443), N487 (= N470), E489 (≠ M472), Q490 (≠ Y473), M492 (≠ T475), V493 (≠ I476), W496 (≠ H479), L518 (= L501), G523 (= G506), L524 (= L507)
- binding methyl 2-[4-methoxy-6-methyl-1,3,5-trazin-2-yl(methyl)carbamoylsulfamoyl]benzoate: G33 (≠ E32), V108 (= V108), P109 (≠ A109), F118 (= F118), K168 (≠ E168), M264 (≠ L259), D289 (≠ E284), R290 (≠ M285), M492 (≠ T475), V493 (≠ I476), W496 (≠ H479)
- binding flavin-adenine dinucleotide: R158 (= R158), G217 (= G213), A218 (≠ G214), G219 (≠ A215), N222 (≠ T218), T244 (≠ S239), L245 (≠ F240), Q246 (≠ R241), L262 (= L257), M264 (≠ L259), H265 (≠ A260), G284 (= G279), A285 (= A280), R286 (= R281), D288 (≠ G283), R290 (≠ M285), V291 (≠ T286), E317 (≠ H306), V318 (≠ A307), N322 (≠ E311), G335 (≠ A324), D336 (≠ S325), A337 (= A326), Q411 (≠ S395), M412 (≠ A396), G430 (≠ L411), G431 (= G412)
- binding magnesium ion: D460 (= D443), N487 (= N470), E489 (≠ M472)
- binding propyl trihydrogen diphosphate: V407 (≠ A391), G408 (= G392), Q409 (≠ N393), H410 (≠ Y394), M435 (= M418), G459 (= G442), D460 (= D443), A461 (≠ G444), S462 (≠ C445), N487 (= N470), E489 (≠ M472), Q490 (≠ Y473), G491 (= G474), M492 (≠ T475)
- binding 5-{[ethyl(methyl)amino]methyl}-2-methyl-5,6-dihydropyrimidin-4-amine: G433 (= G416), M435 (= M418), M465 (= M448)
Sites not aligning to the query:
1t9dA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, metsulfuron methyl (see paper)
29% identity, 96% coverage: 1:539/561 of query aligns to 1:555/596 of 1t9dA
- active site: Y29 (≠ V29), G31 (= G31), G32 (≠ E32), A33 (≠ S33), I34 (≠ Y34), E55 (= E56), T78 (= T79), F117 (= F118), Q118 (= Q119), E119 (= E120), K167 (≠ E168), R227 (≠ A223), M263 (≠ L259), V290 (≠ T286), V406 (≠ A391), L431 (≠ P413), G432 (= G416), M434 (= M418), D459 (= D443), N486 (= N470), E488 (≠ M472), Q489 (≠ Y473), M491 (≠ T475), V492 (≠ I476), W495 (≠ H479), L517 (= L501), G522 (= G506), L523 (= L507)
- binding methyl 2-[({[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: G32 (≠ E32), A33 (≠ S33), V107 (= V108), P108 (≠ A109), F117 (= F118), K167 (≠ E168), M263 (≠ L259), D288 (≠ E284), R289 (≠ M285), W495 (≠ H479)
- binding flavin-adenine dinucleotide: R157 (= R158), G216 (= G213), A217 (≠ G214), G218 (≠ A215), N221 (≠ T218), T243 (≠ S239), L244 (≠ F240), Q245 (≠ R241), M260 (≠ D256), L261 (= L257), H264 (≠ A260), G283 (= G279), A284 (= A280), R285 (= R281), D287 (≠ G283), R289 (≠ M285), V290 (≠ T286), E316 (≠ H306), V317 (≠ A307), N321 (≠ E311), G334 (≠ A324), D335 (≠ S325), A336 (= A326), Q410 (≠ S395), M411 (≠ A396), G429 (≠ L411), G430 (= G412)
- binding magnesium ion: D459 (= D443), N486 (= N470), E488 (≠ M472)
- binding 2,5-dimethyl-pyrimidin-4-ylamine: E55 (= E56), P81 (= P82), Q118 (= Q119), G432 (= G416), M434 (= M418), M464 (= M448)
Sites not aligning to the query:
6u9dB Saccharomyces cerevisiae acetohydroxyacid synthase (see paper)
29% identity, 96% coverage: 1:539/561 of query aligns to 5:566/607 of 6u9dB
- active site: Y33 (≠ V29), G35 (= G31), G36 (≠ E32), A37 (≠ S33), I38 (≠ Y34), E59 (= E56), T82 (= T79), F121 (= F118), Q122 (= Q119), E123 (= E120), K171 (≠ E168), M274 (≠ L259), V301 (≠ T286), V417 (≠ A391), G443 (= G416), M445 (= M418), D470 (= D443), N497 (= N470), E499 (≠ M472), Q500 (≠ Y473), M502 (≠ T475), V503 (≠ I476), W506 (≠ H479)
- binding methyl 2-[(4,6-dimethoxypyrimidin-2-yl)carbamoylsulfamoylmethyl]benzoate: G36 (≠ E32), V111 (= V108), P112 (≠ A109), F121 (= F118), K171 (≠ E168), D299 (≠ E284), R300 (≠ M285), M502 (≠ T475), W506 (≠ H479)
- binding flavin-adenine dinucleotide: R161 (= R158), A228 (≠ G214), G229 (≠ A215), N232 (≠ T218), T254 (≠ S239), L255 (≠ F240), Q256 (≠ R241), L272 (= L257), M274 (≠ L259), G294 (= G279), R296 (= R281), D298 (≠ G283), R300 (≠ M285), V301 (≠ T286), E327 (≠ H306), V328 (≠ A307), N332 (≠ E311), D346 (≠ S325), A347 (= A326), M422 (≠ A396), G440 (≠ L411), G441 (= G412)
- binding magnesium ion: D470 (= D443), N497 (= N470)
- binding thiamine diphosphate: E59 (= E56), P85 (= P82), V417 (≠ A391), G418 (= G392), Q419 (≠ N393), H420 (≠ Y394), G443 (= G416), M445 (= M418), A471 (≠ G444), S472 (≠ C445), N497 (= N470), E499 (≠ M472), Q500 (≠ Y473), G501 (= G474), M502 (≠ T475), V503 (≠ I476)
P07342 Acetolactate synthase catalytic subunit, mitochondrial; Acetohydroxy-acid synthase catalytic subunit; AHAS; ALS; EC 2.2.1.6 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
29% identity, 96% coverage: 1:539/561 of query aligns to 85:646/687 of P07342
- R241 (= R158) binding FAD
- 355:376 (vs. 260:281, 27% identical) binding FAD
- 407:426 (vs. 306:325, 5% identical) binding FAD
1t9bB Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorsulfuron (see paper)
29% identity, 96% coverage: 1:539/561 of query aligns to 1:554/595 of 1t9bB
- active site: Y29 (≠ V29), G31 (= G31), G32 (≠ E32), A33 (≠ S33), I34 (≠ Y34), E55 (= E56), T78 (= T79), F117 (= F118), Q118 (= Q119), E119 (= E120), K167 (≠ E168), R226 (≠ A223), M262 (≠ L259), V289 (≠ T286), V405 (≠ A391), L430 (≠ P413), G431 (= G416), M433 (= M418), D458 (= D443), N485 (= N470), E487 (≠ M472), Q488 (≠ Y473), M490 (≠ T475), V491 (≠ I476), W494 (≠ H479), L516 (= L501), G521 (= G506), L522 (= L507)
- binding 1-(2-chlorophenylsulfonyl)-3-(4-methoxy-6-methyl-l,3,5-triazin-2-yl)urea: V107 (= V108), P108 (≠ A109), D287 (≠ E284), R288 (≠ M285), M490 (≠ T475), W494 (≠ H479)
- binding flavin-adenine dinucleotide: R157 (= R158), G215 (= G213), A216 (≠ G214), G217 (≠ A215), N220 (≠ T218), T242 (≠ S239), L243 (≠ F240), Q244 (≠ R241), M259 (≠ D256), L260 (= L257), M262 (≠ L259), H263 (≠ A260), G282 (= G279), A283 (= A280), R284 (= R281), D286 (≠ G283), R288 (≠ M285), V289 (≠ T286), E315 (≠ H306), V316 (≠ A307), N320 (≠ E311), G333 (≠ A324), D334 (≠ S325), A335 (= A326), Q409 (≠ S395), M410 (≠ A396), G428 (≠ L411), G429 (= G412)
- binding magnesium ion: D458 (= D443), N485 (= N470), E487 (≠ M472)
Sites not aligning to the query:
1t9bA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorsulfuron (see paper)
29% identity, 96% coverage: 1:539/561 of query aligns to 1:542/583 of 1t9bA
- active site: Y29 (≠ V29), G31 (= G31), G32 (≠ E32), A33 (≠ S33), I34 (≠ Y34), E55 (= E56), T78 (= T79), F117 (= F118), Q118 (= Q119), E119 (= E120), K167 (≠ E168), R214 (≠ A223), M250 (≠ L259), V277 (≠ T286), V393 (≠ A391), L418 (≠ P413), G419 (= G416), M421 (= M418), D446 (= D443), N473 (= N470), E475 (≠ M472), Q476 (≠ Y473), M478 (≠ T475), V479 (≠ I476), W482 (≠ H479), L504 (= L501), G509 (= G506), L510 (= L507)
- binding 1-(2-chlorophenylsulfonyl)-3-(4-methoxy-6-methyl-l,3,5-triazin-2-yl)urea: V107 (= V108), P108 (≠ A109), F117 (= F118), D275 (≠ E284), R276 (≠ M285), M478 (≠ T475), W482 (≠ H479)
- binding flavin-adenine dinucleotide: R157 (= R158), G203 (= G213), A204 (≠ G214), G205 (≠ A215), N208 (≠ T218), T230 (≠ S239), L231 (≠ F240), Q232 (≠ R241), M247 (≠ D256), L248 (= L257), M250 (≠ L259), H251 (≠ A260), G270 (= G279), A271 (= A280), R272 (= R281), D274 (≠ G283), R276 (≠ M285), V277 (≠ T286), E303 (≠ H306), V304 (≠ A307), N308 (≠ E311), D322 (≠ S325), A323 (= A326), Q397 (≠ S395), M398 (≠ A396), G416 (≠ L411), G417 (= G412)
- binding magnesium ion: D446 (= D443), N473 (= N470), E475 (≠ M472)
Sites not aligning to the query:
1t9dB Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, metsulfuron methyl (see paper)
29% identity, 95% coverage: 7:539/561 of query aligns to 6:541/582 of 1t9dB
- active site: Y28 (≠ V29), G30 (= G31), G31 (≠ E32), A32 (≠ S33), I33 (≠ Y34), E54 (= E56), T77 (= T79), F116 (= F118), Q117 (= Q119), E118 (= E120), K166 (≠ E168), R213 (≠ A223), M249 (≠ L259), V276 (≠ T286), V392 (≠ A391), L417 (≠ P413), G418 (= G416), M420 (= M418), D445 (= D443), N472 (= N470), E474 (≠ M472), Q475 (≠ Y473), M477 (≠ T475), V478 (≠ I476), W481 (≠ H479), L503 (= L501), G508 (= G506), L509 (= L507)
- binding methyl 2-[({[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: G31 (≠ E32), A32 (≠ S33), V106 (= V108), P107 (≠ A109), F116 (= F118), K166 (≠ E168), M249 (≠ L259), D274 (≠ E284), R275 (≠ M285), W481 (≠ H479)
- binding flavin-adenine dinucleotide: R156 (= R158), G202 (= G213), A203 (≠ G214), G204 (≠ A215), N207 (≠ T218), T229 (≠ S239), L230 (≠ F240), Q231 (≠ R241), L247 (= L257), M249 (≠ L259), H250 (≠ A260), G269 (= G279), A270 (= A280), R271 (= R281), D273 (≠ G283), R275 (≠ M285), V276 (≠ T286), E302 (≠ H306), V303 (≠ A307), N307 (≠ E311), G320 (≠ A324), D321 (≠ S325), A322 (= A326), Q396 (≠ S395), M397 (≠ A396), G415 (≠ L411), G416 (= G412)
- binding magnesium ion: D445 (= D443), N472 (= N470), E474 (≠ M472)
- binding 2,5-dimethyl-pyrimidin-4-ylamine: E54 (= E56), P80 (= P82), G418 (= G416), M420 (= M418), M450 (= M448)
Sites not aligning to the query:
6deoA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide iodomuron methyl (see paper)
28% identity, 95% coverage: 7:539/561 of query aligns to 9:552/593 of 6deoA
- active site: Y31 (≠ V29), G33 (= G31), G34 (≠ E32), A35 (≠ S33), I36 (≠ Y34), E57 (= E56), T80 (= T79), F119 (= F118), Q120 (= Q119), E121 (= E120), K169 (≠ E168), K224 (≠ A223), M260 (≠ L259), V287 (≠ T286), V403 (≠ A391), L428 (vs. gap), G429 (= G416), M431 (= M418), D456 (= D443), N483 (= N470), E485 (≠ M472), Q486 (≠ Y473), M488 (≠ T475), V489 (≠ I476), W492 (≠ H479), L514 (= L501), N519 (≠ G506), V520 (≠ L507)
- binding flavin-adenine dinucleotide: R159 (= R158), G213 (= G213), A214 (vs. gap), G215 (= G214), N218 (≠ W217), T240 (≠ S239), L241 (≠ F240), Q242 (≠ R241), L258 (= L257), G280 (= G279), A281 (= A280), R282 (= R281), D284 (≠ G283), R286 (≠ M285), V287 (≠ T286), E313 (≠ H306), I314 (≠ A307), N318 (≠ E311), D332 (≠ S325), V333 (≠ A326), M408 (≠ A396), G426 (≠ T414)
- binding methyl 2-{[(4-iodo-6-methoxypyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: M260 (≠ L259), D285 (≠ E284), R286 (≠ M285), M488 (≠ T475), W492 (≠ H479)
- binding magnesium ion: D456 (= D443), N483 (= N470), E485 (≠ M472)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V403 (≠ A391), G404 (= G392), Q405 (≠ N393), H406 (≠ Y394), G429 (= G416), M431 (= M418), G455 (= G442), D456 (= D443), A457 (≠ G444), S458 (≠ C445), M461 (= M448), N483 (= N470), E485 (≠ M472), Q486 (≠ Y473), G487 (= G474), M488 (≠ T475), V489 (≠ I476)
6deqA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide penoxsulam (see paper)
29% identity, 95% coverage: 7:539/561 of query aligns to 13:560/601 of 6deqA
- active site: Y35 (≠ V29), G37 (= G31), G38 (≠ E32), A39 (≠ S33), I40 (≠ Y34), E61 (= E56), T84 (= T79), F123 (= F118), Q124 (= Q119), E125 (= E120), K173 (≠ E168), K232 (≠ A223), M268 (≠ L259), V295 (≠ T286), V411 (≠ A391), L436 (vs. gap), G437 (= G416), M439 (= M418), D464 (= D443), N491 (= N470), E493 (≠ M472), Q494 (≠ Y473), M496 (≠ T475), V497 (≠ I476), W500 (≠ H479), L522 (= L501), N527 (≠ G506), V528 (≠ L507)
- binding flavin-adenine dinucleotide: R163 (= R158), G221 (= G213), A222 (vs. gap), G223 (= G214), N226 (≠ W217), T248 (≠ S239), L249 (≠ F240), Q250 (≠ R241), L266 (= L257), G288 (= G279), A289 (= A280), R290 (= R281), D292 (≠ G283), R294 (≠ M285), V295 (≠ T286), E321 (≠ H306), I322 (≠ A307), D340 (≠ S325), V341 (≠ A326), M416 (≠ A396), G434 (≠ T414)
- binding magnesium ion: D464 (= D443), N491 (= N470), E493 (≠ M472)
- binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: M268 (≠ L259), R294 (≠ M285), M496 (≠ T475), V497 (≠ I476), W500 (≠ H479)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V411 (≠ A391), G412 (= G392), Q413 (≠ N393), H414 (≠ Y394), M439 (= M418), G463 (= G442), D464 (= D443), A465 (≠ G444), S466 (≠ C445), N491 (= N470), E493 (≠ M472), Q494 (≠ Y473), G495 (= G474), M496 (≠ T475), V497 (≠ I476)
Sites not aligning to the query:
5imsA Saccharomyces cerevisiae acetohydroxyacid synthase
28% identity, 96% coverage: 1:539/561 of query aligns to 3:540/541 of 5imsA
- active site: Y31 (≠ V29), G33 (= G31), G34 (≠ E32), A35 (≠ S33), I36 (≠ Y34), E57 (= E56), T80 (= T79), F119 (= F118), Q120 (= Q119), E121 (= E120), K169 (≠ E168), R229 (≠ A223), M265 (≠ L259), V292 (≠ T286), V408 (≠ A391), L433 (≠ P413), G434 (= G416), M436 (= M418), D461 (= D443), N488 (= N470), E490 (≠ R482), L502 (= L501), G507 (= G506), L508 (= L507)
- binding flavin-adenine dinucleotide: R159 (= R158), G218 (= G213), A219 (≠ G214), G220 (≠ A215), N223 (≠ T218), T245 (≠ S239), L246 (≠ F240), L263 (= L257), G264 (= G258), H266 (≠ A260), G285 (= G279), A286 (= A280), R287 (= R281), D289 (≠ G283), R291 (≠ M285), V292 (≠ T286), E318 (≠ H306), V319 (≠ A307), N323 (≠ E311), D337 (≠ S325), A338 (= A326)
- binding magnesium ion: D461 (= D443), N488 (= N470)
- binding oxygen molecule: G34 (≠ E32), T80 (= T79), Q120 (= Q119)
- binding thiamine diphosphate: P32 (= P30), E57 (= E56), V408 (≠ A391), G409 (= G392), Q410 (≠ N393), H411 (≠ Y394), G434 (= G416), M436 (= M418), G460 (= G442), D461 (= D443), A462 (≠ G444), S463 (≠ C445), M466 (= M448), N488 (= N470)
Sites not aligning to the query:
1jscA Crystal structure of the catalytic subunit of yeast acetohydroxyacid synthase: a target for herbicidal inhibitors (see paper)
28% identity, 96% coverage: 1:539/561 of query aligns to 3:539/541 of 1jscA
- active site: Y31 (≠ V29), G33 (= G31), G34 (≠ E32), A35 (≠ S33), I36 (≠ Y34), E57 (= E56), T80 (= T79), F119 (= F118), Q120 (= Q119), E121 (= E120), K169 (≠ E168), M263 (≠ L259), V290 (≠ T286), V406 (≠ A391), G432 (= G416), M434 (= M418), D459 (= D443), N486 (= N470), E488 (= E481)
- binding dihydrogenphosphate ion: G33 (= G31), G34 (≠ E32), Q120 (= Q119)
- binding flavin-adenine dinucleotide: R159 (= R158), G216 (= G213), A217 (≠ G214), G218 (≠ A215), N221 (≠ T218), T243 (≠ S239), L244 (≠ F240), L261 (= L257), G262 (= G258), H264 (≠ A260), G283 (= G279), A284 (= A280), R285 (= R281), D287 (≠ G283), R289 (≠ M285), V290 (≠ T286), E316 (≠ H306), V317 (≠ A307), N321 (≠ E311), G334 (≠ A324), D335 (≠ S325), A336 (= A326)
- binding magnesium ion: D459 (= D443), N486 (= N470)
- binding thiamine diphosphate: Y31 (≠ V29), P32 (= P30), E57 (= E56), P83 (= P82), V406 (≠ A391), G407 (= G392), Q408 (≠ N393), H409 (≠ Y394), M434 (= M418), D459 (= D443), A460 (≠ G444), S461 (≠ C445), N486 (= N470)
Sites not aligning to the query:
6bd9A Saccharomyces cerevisiae acetohydroxyacid synthase
28% identity, 96% coverage: 1:539/561 of query aligns to 3:540/542 of 6bd9A
- active site: Y31 (≠ V29), G33 (= G31), G34 (≠ E32), A35 (≠ S33), I36 (≠ Y34), E57 (= E56), T80 (= T79), F119 (= F118), Q120 (= Q119), E121 (= E120), K169 (≠ E168), R228 (≠ A223), M264 (≠ L259), V291 (≠ T286), V407 (≠ A391), L432 (≠ P413), G433 (= G416), M435 (= M418), D460 (= D443), N487 (= N470), E489 (= E481), L502 (= L501), G507 (= G506), L508 (= L507)
- binding flavin-adenine dinucleotide: R159 (= R158), G217 (= G213), A218 (≠ G214), G219 (≠ A215), N222 (≠ T218), T244 (≠ S239), L245 (≠ F240), L262 (= L257), G263 (= G258), H265 (≠ A260), G284 (= G279), A285 (= A280), R286 (= R281), D288 (≠ G283), R290 (≠ M285), V291 (≠ T286), E317 (≠ H306), V318 (≠ A307), N322 (≠ E311), G335 (≠ A324), D336 (≠ S325), A337 (= A326)
- binding magnesium ion: D460 (= D443), N487 (= N470)
- binding oxygen molecule: G34 (≠ E32), T80 (= T79), Q120 (= Q119), A461 (≠ G444), Q494 (≠ L493)
- binding pyruvic acid: G33 (= G31), G34 (≠ E32), G34 (≠ E32), A35 (≠ S33), Q120 (= Q119)
- binding thiamine diphosphate: P32 (= P30), E57 (= E56), V407 (≠ A391), G408 (= G392), Q409 (≠ N393), H410 (≠ Y394), G433 (= G416), M435 (= M418), G459 (= G442), D460 (= D443), A461 (≠ G444), S462 (≠ C445), M465 (= M448), N487 (= N470)
Sites not aligning to the query:
6derA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide metosulam (see paper)
28% identity, 95% coverage: 7:539/561 of query aligns to 13:559/600 of 6derA
- active site: Y35 (≠ V29), G37 (= G31), G38 (≠ E32), A39 (≠ S33), I40 (≠ Y34), E61 (= E56), T84 (= T79), F123 (= F118), Q124 (= Q119), E125 (= E120), K173 (≠ E168), K231 (≠ A223), M267 (≠ L259), V294 (≠ T286), V410 (≠ A391), L435 (vs. gap), G436 (= G416), M438 (= M418), D463 (= D443), N490 (= N470), E492 (≠ M472), Q493 (≠ Y473), M495 (≠ T475), V496 (≠ I476), W499 (≠ H479), L521 (= L501), N526 (≠ G506), V527 (≠ L507)
- binding flavin-adenine dinucleotide: R163 (= R158), G220 (= G213), A221 (vs. gap), G222 (= G214), N225 (≠ W217), T247 (≠ S239), L248 (≠ F240), Q249 (≠ R241), L265 (= L257), H268 (≠ A260), G287 (= G279), A288 (= A280), R289 (= R281), D291 (≠ G283), R293 (≠ M285), V294 (≠ T286), E320 (≠ H306), I321 (≠ A307), N325 (≠ E311), G338 (≠ A324), D339 (≠ S325), V340 (≠ A326), Q414 (≠ S395), M415 (≠ A396), G433 (≠ T414)
- binding Metosulam: R293 (≠ M285), M495 (≠ T475), W499 (≠ H479)
- binding magnesium ion: D463 (= D443), N490 (= N470), E492 (≠ M472)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V410 (≠ A391), G411 (= G392), Q412 (≠ N393), H413 (≠ Y394), G436 (= G416), M438 (= M418), G462 (= G442), D463 (= D443), A464 (≠ G444), S465 (≠ C445), N490 (= N470), E492 (≠ M472), Q493 (≠ Y473), G494 (= G474), M495 (≠ T475), V496 (≠ I476)
- binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V410 (≠ A391), G411 (= G392), Q412 (≠ N393), H413 (≠ Y394), G436 (= G416), M438 (= M418), G462 (= G442), D463 (= D443), A464 (≠ G444), S465 (≠ C445), M468 (= M448), N490 (= N470), E492 (≠ M472), Q493 (≠ Y473), G494 (= G474), V496 (≠ I476)
Sites not aligning to the query:
6desA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide propoxycarbazone (see paper)
28% identity, 95% coverage: 7:539/561 of query aligns to 11:557/598 of 6desA
- active site: Y33 (≠ V29), G35 (= G31), G36 (≠ E32), A37 (≠ S33), I38 (≠ Y34), E59 (= E56), T82 (= T79), F121 (= F118), Q122 (= Q119), E123 (= E120), K171 (≠ E168), K229 (≠ A223), M265 (≠ L259), V292 (≠ T286), V408 (≠ A391), L433 (vs. gap), G434 (= G416), M436 (= M418), D461 (= D443), N488 (= N470), E490 (≠ M472), Q491 (≠ Y473), M493 (≠ T475), V494 (≠ I476), W497 (≠ H479), L519 (= L501), N524 (≠ G506), V525 (≠ L507)
- binding methyl 2-[(4-methyl-5-oxidanylidene-3-propoxy-1,2,4-triazol-1-yl)carbonylsulfamoyl]benzoate: M265 (≠ L259), D290 (≠ E284), R291 (≠ M285), W497 (≠ H479)
- binding flavin-adenine dinucleotide: R161 (= R158), G218 (= G213), A219 (vs. gap), G220 (= G214), N223 (≠ W217), T245 (≠ S239), L246 (≠ F240), Q247 (≠ R241), L263 (= L257), G285 (= G279), A286 (= A280), R287 (= R281), D289 (≠ G283), R291 (≠ M285), V292 (≠ T286), E318 (≠ H306), I319 (≠ A307), N323 (≠ E311), D337 (≠ S325), V338 (≠ A326), Q412 (≠ S395), M413 (≠ A396), G431 (≠ T414)
- binding magnesium ion: D461 (= D443), N488 (= N470), E490 (≠ M472)
- binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V408 (≠ A391), G409 (= G392), Q410 (≠ N393), H411 (≠ Y394), G434 (= G416), M436 (= M418), G460 (= G442), D461 (= D443), A462 (≠ G444), S463 (≠ C445), N488 (= N470), E490 (≠ M472), Q491 (≠ Y473), G492 (= G474), M493 (≠ T475), V494 (≠ I476)
6depA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide sulfometuron methyl (see paper)
28% identity, 95% coverage: 7:539/561 of query aligns to 11:557/598 of 6depA
- active site: Y33 (≠ V29), G35 (= G31), G36 (≠ E32), A37 (≠ S33), I38 (≠ Y34), E59 (= E56), T82 (= T79), F121 (= F118), Q122 (= Q119), E123 (= E120), K171 (≠ E168), K229 (≠ A223), M265 (≠ L259), V292 (≠ T286), V408 (≠ A391), L433 (vs. gap), G434 (= G416), M436 (= M418), D461 (= D443), N488 (= N470), E490 (≠ M472), Q491 (≠ Y473), M493 (≠ T475), V494 (≠ I476), W497 (≠ H479), L519 (= L501), N524 (≠ G506), V525 (≠ L507)
- binding methyl 2-[({[(4,6-dimethylpyrimidin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D290 (≠ E284), R291 (≠ M285), M493 (≠ T475), W497 (≠ H479)
- binding flavin-adenine dinucleotide: R161 (= R158), G218 (= G213), A219 (vs. gap), G220 (= G214), N223 (≠ W217), T245 (≠ S239), L246 (≠ F240), Q247 (≠ R241), L263 (= L257), G264 (= G258), G285 (= G279), A286 (= A280), R287 (= R281), D289 (≠ G283), R291 (≠ M285), V292 (≠ T286), E318 (≠ H306), I319 (≠ A307), N323 (≠ E311), D337 (≠ S325), V338 (≠ A326), M413 (≠ A396), G431 (≠ T414)
- binding magnesium ion: D461 (= D443), N488 (= N470), E490 (≠ M472)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V408 (≠ A391), G409 (= G392), Q410 (≠ N393), H411 (≠ Y394), G434 (= G416), M436 (= M418), G460 (= G442), D461 (= D443), A462 (≠ G444), S463 (≠ C445), M466 (= M448), N488 (= N470), E490 (≠ M472), Q491 (≠ Y473), G492 (= G474), M493 (≠ T475), V494 (≠ I476)
- binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V408 (≠ A391), G409 (= G392), Q410 (≠ N393), H411 (≠ Y394), G434 (= G416), M436 (= M418), G460 (= G442), D461 (= D443), A462 (≠ G444), S463 (≠ C445), M466 (= M448), N488 (= N470), E490 (≠ M472), Q491 (≠ Y473), G492 (= G474), M493 (≠ T475), V494 (≠ I476)
6demA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide bensulfuron methyl (see paper)
28% identity, 95% coverage: 7:539/561 of query aligns to 11:556/597 of 6demA
- active site: Y33 (≠ V29), G35 (= G31), G36 (≠ E32), A37 (≠ S33), I38 (≠ Y34), E59 (= E56), T82 (= T79), F121 (= F118), Q122 (= Q119), E123 (= E120), K171 (≠ E168), K228 (≠ A223), M264 (≠ L259), V291 (≠ T286), V407 (≠ A391), L432 (vs. gap), G433 (= G416), M435 (= M418), D460 (= D443), N487 (= N470), E489 (≠ M472), Q490 (≠ Y473), M492 (≠ T475), V493 (≠ I476), W496 (≠ H479), L518 (= L501), N523 (≠ G506), V524 (≠ L507)
- binding methyl 2-[(4,6-dimethoxypyrimidin-2-yl)carbamoylsulfamoylmethyl]benzoate: M264 (≠ L259), D289 (≠ E284), R290 (≠ M285), M492 (≠ T475), W496 (≠ H479)
- binding flavin-adenine dinucleotide: R161 (= R158), G217 (= G213), A218 (vs. gap), G219 (= G214), N222 (≠ W217), T244 (≠ S239), L245 (≠ F240), Q246 (≠ R241), L262 (= L257), G284 (= G279), A285 (= A280), R286 (= R281), D288 (≠ G283), R290 (≠ M285), V291 (≠ T286), E317 (≠ H306), I318 (≠ A307), N322 (≠ E311), D336 (≠ S325), V337 (≠ A326), M412 (≠ A396), G430 (≠ T414)
- binding magnesium ion: D460 (= D443), N487 (= N470), E489 (≠ M472)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V407 (≠ A391), G408 (= G392), Q409 (≠ N393), H410 (≠ Y394), M435 (= M418), G459 (= G442), D460 (= D443), A461 (≠ G444), S462 (≠ C445), M465 (= M448), N487 (= N470), E489 (≠ M472), Q490 (≠ Y473), G491 (= G474), M492 (≠ T475), V493 (≠ I476)
Sites not aligning to the query:
6delA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide chlorimuron ethyl (see paper)
28% identity, 95% coverage: 7:539/561 of query aligns to 11:556/597 of 6delA
- active site: Y33 (≠ V29), G35 (= G31), G36 (≠ E32), A37 (≠ S33), I38 (≠ Y34), E59 (= E56), T82 (= T79), F121 (= F118), Q122 (= Q119), E123 (= E120), K171 (≠ E168), K228 (≠ A223), M264 (≠ L259), V291 (≠ T286), V407 (≠ A391), L432 (vs. gap), G433 (= G416), M435 (= M418), D460 (= D443), N487 (= N470), E489 (≠ M472), Q490 (≠ Y473), M492 (≠ T475), V493 (≠ I476), W496 (≠ H479), L518 (= L501), N523 (≠ G506), V524 (≠ L507)
- binding 2-[[[[(4-chloro-6-methoxy-2-pyrimidinyl)amino]carbonyl]amino]sulfonyl]benzoic acid ethyl ester: D289 (≠ E284), R290 (≠ M285), W496 (≠ H479)
- binding flavin-adenine dinucleotide: R161 (= R158), G217 (= G213), A218 (vs. gap), G219 (= G214), N222 (≠ W217), T244 (≠ S239), L245 (≠ F240), Q246 (≠ R241), L262 (= L257), G284 (= G279), A285 (= A280), R286 (= R281), D288 (≠ G283), R290 (≠ M285), V291 (≠ T286), E317 (≠ H306), I318 (≠ A307), N322 (≠ E311), D336 (≠ S325), V337 (≠ A326), M412 (≠ A396), G430 (≠ T414)
- binding (3Z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl](formyl)amino}-3-sulfanylpent-3-en-1-yl trihydrogen diphosphate: V407 (≠ A391), G408 (= G392), Q409 (≠ N393), H410 (≠ Y394), G433 (= G416), M435 (= M418), G459 (= G442), D460 (= D443), A461 (≠ G444), S462 (≠ C445), M465 (= M448), N487 (= N470), E489 (≠ M472), Q490 (≠ Y473), G491 (= G474), M492 (≠ T475), V493 (≠ I476)
- binding magnesium ion: D460 (= D443), N487 (= N470), E489 (≠ M472)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V407 (≠ A391), G408 (= G392), Q409 (≠ N393), H410 (≠ Y394), G433 (= G416), M435 (= M418), G459 (= G442), D460 (= D443), A461 (≠ G444), S462 (≠ C445), M465 (= M448), N487 (= N470), E489 (≠ M472), Q490 (≠ Y473), G491 (= G474), M492 (≠ T475), V493 (≠ I476)
Query Sequence
>WP_012706368.1 NCBI__GCF_000018545.1:WP_012706368.1
MTTGTKGRNGGQILIDALRIHGVERAFCVPGESYLAALDALHEAKDDIELVVCRQEGGAA
YMAEAYGKLTGKPGICFVTRGPGATNASVGVHTAFQDSTPTILFIGQVARDQVEREAFQE
VDYRRMFGQMAKWVVQIDDAARIPELVSQAFHRAVNGRPGPIVVALPEDMLIDCVDVADA
PAYKTVEMHAGQAQLDELRSRLEQAERPIVIVGGAGWTEDAVADIRSFSEAMRLPVAASF
RCQDLFDNTHPNYAGDLGLAAGPKLIQRIKDSDLLIVIGARLGEMTTGGYSLIDIPVPKQ
TLVHVHAGAEELGRVYHADLPINASAAAFARQAAGLAPIAEPRWAEWTRGANADYRENLE
HPAVPGPVQMGDIMAWLRDHLPADAILTTGAGNYSAWAHRFYQYRTFRTQLGPTNGSMGY
GVPAAIAAKLSAPERPVIAFAGDGCFLMNGQELATAMQYDARVIVLVINNGMYGTIRMHQ
ERHYPGRVSGTQLTNPDFAALARAYGLHGETVETTEDFAPAFERAEQSGKPALIEVRIDP
EALTPKMSLTQIRDQALAQGT
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory