SitesBLAST

O43175 D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; Malate dehydrogenase; EC 1.1.1.95; EC 1.1.1.399; EC 1.1.1.37 from Homo sapiens (Human) (see 3 papers)
34% identity, 88% coverage: 34:320/327 of query aligns to 32:321/533 of O43175

query
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O43175

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T78 (≠ V79) binding NAD(+)
R135 (= R136) to W: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606949
RI 155:156 (≠ II 157:158) binding NAD(+)
D175 (= D177) binding NAD(+)
T207 (≠ A206) binding NAD(+)
CAR 234:236 (≠ TSR 233:235) binding NAD(+)
D260 (= D259) binding NAD(+)
V261 (= V260) to M: in PHGDHD; results in a four-fold decrease in substrate affinity and a slight increase in maximal enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs267606947
HLGA 283:286 (≠ HIGG 282:285) binding NAD(+)

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine
373 A → T: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs201553627
377 G → S: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606948
425 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907988
490 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907987

6plfA Crystal structure of human phgdh complexed with compound 1 (see paper)
34% identity, 84% coverage: 34:308/327 of query aligns to 28:305/305 of 6plfA

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binding 4-{(1S)-1-[(5-chloro-6-{[(5S)-2-oxo-1,3-oxazolidin-5-yl]methoxy}-1H-indole-2-carbonyl)amino]-2-hydroxyethyl}benzoic acid: Y170 (≠ F176), D171 (= D177), P172 (≠ A178), L189 (= L192), H202 (= H205), T203 (≠ A206), P204 (= P207), L212 (= L215)

6rj5A Crystal structure of phgdh in complex with compound 39 (see paper)
35% identity, 80% coverage: 34:293/327 of query aligns to 27:289/301 of 6rj5A

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binding 2-methyl-~{N}-[(1~{R})-1-[4-(methylsulfonylcarbamoyl)phenyl]ethyl]-5-phenyl-pyrazole-3-carboxamide: L146 (≠ I153), Y169 (≠ F176), D170 (= D177), P171 (≠ A178), I173 (vs. gap), H201 (= H205), T202 (≠ A206), P203 (= P207), L211 (= L215)

6plgA Crystal structure of human phgdh complexed with compound 15 (see paper)
35% identity, 80% coverage: 34:293/327 of query aligns to 27:289/303 of 6plgA

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6plgA

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binding (2S)-(4-{3-[(4,5-dichloro-1-methyl-1H-indole-2-carbonyl)amino]oxetan-3-yl}phenyl)(pyridin-3-yl)acetic acid: G149 (= G156), R150 (≠ I157), D170 (= D177), P171 (≠ A178), I172 (≠ M179), I173 (vs. gap), H201 (= H205), T202 (≠ A206), L205 (= L209)

7ewhA Crystal structure of human phgdh in complex with homoharringtonine (see paper)
35% identity, 80% coverage: 34:293/327 of query aligns to 27:289/302 of 7ewhA

query
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7ewhA

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L

T

S

V

A

A

A

D

E

Q

L

T

T

L

C

A

G

M

M

I

I

L

M

G

C

L

L

M

A

R

R

R

Q

V

I

V

P

T

Q

Q

A

D

T

Q

A

A

S

V

M

K

K

A

D

G

G

R

K

W

W

D

E

R

R

V

-

G

K

E

K

A

F

T

M

P

G

T

T

E

E

L

L

Y

N

R

G

K

K

T

T

V

L

G

G

L

I

I
x
L

G
|
G

A
x
L

G
|
G

I
x
R

I
|
I

G
|
G

K

R

A

E

V

V

I

A

R

T

R

R

L

M

L

Q

G

S

F

F

G

G

V

M

R

K

V

T

L

I

Y

G

F

Y

D
|
D

A

P

M

I

-

I

-

S

-

P

-

E

V

V

E

S

K

A

V

S

H

F

G

G

A

V

E

Q

R

Q

C

L

G

-

S

P

L

L

D

E

Q

E

L

I

L

W

G

P

S

L

S

C

D

D

I

F

V

I

S

T

L

V

H
|
H

A
x
T

P
|
P

L

L

A

P

D

S

T

T

R

T

E

G

L

L

M

L

N

N

A

D

A

N

R

T

I

F

A

A

L

Q

M

C

P

K

K

K

G

G

S

V

Y

R

L

V

I

V

N

N

T

C

S

A

R

R

G

G

L

I

V

V

Q

D

Q

E

P

G

A

A

V

L

F

L

A

R

A

A

L

L

R

Q

S

S

G

G

H

Q

L

C

A

A

G

G

A

A

L

L

D

D

V

V

F

F

E

T

V

E

E

E

P

P

G

R

A

D

E

R

A

A

L

L

A

V

D

D

V

H

P

E

N

N

L

V

I

I

A

S

S

C

A

P

H

H

I

L

G

G

G

A

I

S

S

T

T

K

E

E

S

A

I

Q

A

S

R

R

binding (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine: L146 (≠ I153), G147 (= G154), L148 (≠ A155), G149 (= G156), R150 (≠ I157), I151 (= I158), G152 (= G159), D170 (= D177), H201 (= H205), T202 (≠ A206), P203 (= P207)

6rihA Crystal structure of phgdh in complex with compound 9 (see paper)
35% identity, 80% coverage: 34:293/327 of query aligns to 27:289/302 of 6rihA

query
sites
6rihA

K

E

R

K

G

Q

N

N

R

L

T

S

A

K

D

E

E

E

L

L

A

I

T

A

L

E

L

L

E

Q

D

D

C

C

D

E

A

G

A

L

I

I

V

V

-

R

S

S

T

A

D

T

P

K

F

V

T

T

R

A

E

D

V

V

L

I

A

N

G

A

D

A

R

E

N

K

L

L

K

Q

V

V

I

V

A

G

R

R

V

A

G

G

V

T

G

G

T

V

D

D

S

N

I

V

D

D

H

L

D

E

A

A

K

T

E

R

F

K

G

G

V

I

G

L

I

V

S

M

V

N

T

T

P

P

G

N

M

G

N

N

A

S

E

L

T

S

V

A

A

A

D

E

Q

L

T

T

L

C

A

G

M

M

I

I

L

M

G

C

L

L

M

A

R

R

R

Q

V

I

V

P

T

Q

Q

A

D

T

Q

A

A

S

V

M

K

K

A

D

G

G

R

K

W

W

D

E

R

R

V

-

G

K

E

K

A

F

T

M

P

G

T

T

E

E

L

L

Y

N

R

G

K

K

T

T

V

L

G

G

L

I

I

L

G

G

A

L

G
|
G

I

R

I

I

G

G

K

R

A

E

V

V

I

A

R

T

R

R

L

M

L

Q

G

S

F

F

G

G

V

M

R

K

V

T

L

I

Y

G

F
x
Y

D
|
D

A
x
P

M
x
I

-
x
I

-

S

-

P

-

E

V

V

E

S

K

A

V

S

H

F

G

G

A

V

E

Q

R

Q

C

L

G

-

S

P

L

L

D

E

Q

E

L

I

L

W

G

P

S

L

S

C

D

D

I

F

V

I

S

T

L

V

H

H

A
x
T

P
|
P

L

L

A

P

D

S

T

T

R

T

E

G

L
|
L

M

L

N

N

A

D

A

N

R

T

I

F

A

A

L

Q

M

C

P

K

K

K

G

G

S

V

Y

R

L

V

I

V

N

N

T

C

S

A

R

R

G

G

L

I

V

V

Q

D

Q

E

P

G

A

A

V

L

F

L

A

R

A

A

L

L

R

Q

S

S

G

G

H

Q

L

C

A

A

G

G

A

A

L

L

D

D

V

V

F

F

E

T

V

E

E

E

P

P

G

R

A

D

E

R

A

A

L

L

A

V

D

D

V

H

P

E

N

N

L

V

I

I

A

S

S

C

A

P

H

H

I

L

G

G

G

A

I

S

S

T

T

K

E

E

S

A

I

Q

A

S

R

R

binding ~{N}-cyclopropyl-2-methyl-5-phenyl-pyrazole-3-carboxamide: G149 (= G156), Y169 (≠ F176), D170 (= D177), P171 (≠ A178), I172 (≠ M179), I173 (vs. gap), T202 (≠ A206), P203 (= P207), L211 (= L215)

6cwaA Crystal structure phgdh in complex with nadh and 3-phosphoglycerate at 1.77 a resolution (see paper)
35% identity, 80% coverage: 34:293/327 of query aligns to 26:288/299 of 6cwaA

query
sites
6cwaA

K

E

R

K

G

Q

N

N

R

L

T

S

A

K

D

E

E

E

L

L

A

I

T

A

L

E

L

L

E

Q

D

D

C

C

D

E

A

G

A

L

I

I

V

V

-

R

S

S

T

A

D

T

P

K

F

V

T

T

R

A

E

D

V

V

L

I

A

N

G

A

D

A

R

E

N

K

L

L

K

Q

V

V

I

V

A

G

R

R

V

A

G

G

V

T

G

G

T

V

D

D

S

N

I

V

D

D

H

L

D

E

A

A

K

T

E

R

F

K

G

G

V

I

G

L

I

V

S

M

V

N

T

T

P

P

G

N

M

G

N
|
N

A

S

E

L

T

S

V
x
A

A

A

D

E

Q

L

T

T

L

C

A

G

M

M

I

I

L

M

G

C

L

L

M

A

R

R

R

Q

V

I

V

P

T

Q

Q

A

D

T

Q

A

A

S

V

M

K

K

A

D

G

G

R

K

W

W

D

E

R

R

V

-

G

K

E

K

A

F

T

M

P

G

T

T

E

E

L

L

Y

N

R

G

K

K

T

T

V

L

G

G

L

I

I

L

G

G

A

L

G

G

I
x
R

I
|
I

G

G

K

R

A

E

V

V

I

A

R

T

R

R

L

M

L

Q

G

S

F

F

G

G

V

M

R

K

V

T

L

I

Y

G

F
x
Y

D
|
D

A
x
P

M
x
I

-

I

-

S

-

P

-

E

V

V

E

S

K

A

V

S

H

F

G

G

A

V

E

Q

R

Q

C

L

G

-

S

P

L

L

D

E

Q

E

L

I

L

W

G

P

S

L

S

C

D

D

I

F

V

I

S

T

L

V

H
|
H

A
x
T

P
|
P

L

L

A

P

D

S

T
|
T

R

T

E

G

L

L

M

L

N

N

A

D

A

N

R

T

I

F

A

A

L

Q

M

C

P

K

K

K

G

G

S

V

Y

R

L

V

I

V

N

N

T
x
C

S
x
A

R
|
R

G

G

L

I

V

V

Q

D

Q

E

P

G

A

A

V

L

F

L

A

R

A

A

L

L

R

Q

S

S

G

G

H

Q

L

C

A

A

G

G

A

A

L

L

D

D

V

V

F

F

E

T

V

E

E

E

P

P

G

R

A

D

E

R

A

A

L

L

A

V

D

D

V

H

P

E

N

N

L

V

I

I

A

S

S

C

A

P

H
|
H

I

L

G
|
G

G

A

I

S

S

T

T

K

E

E

S

A

I

Q

A

S

R

R

binding 1,4-dihydronicotinamide adenine dinucleotide: N96 (= N103), A100 (≠ V107), R149 (≠ I157), I150 (= I158), Y168 (≠ F176), D169 (= D177), P170 (≠ A178), I171 (≠ M179), H200 (= H205), T201 (≠ A206), P202 (= P207), T207 (= T212), C228 (≠ T233), A229 (≠ S234), R230 (= R235), H277 (= H282), G279 (= G284)

6rj3A Crystal structure of phgdh in complex with compound 15 (see paper)
35% identity, 80% coverage: 34:293/327 of query aligns to 26:288/297 of 6rj3A

query
sites
6rj3A

K

E

R

K

G

Q

N

N

R

L

T

S

A

K

D

E

E

E

L

L

A

I

T

A

L

E

L

L

E

Q

D

D

C

C

D

E

A

G

A

L

I

I

V

V

-

R

S

S

T

A

D

T

P

K

F

V

T

T

R

A

E

D

V

V

L

I

A

N

G

A

D

A

R

E

N

K

L

L

K

Q

V

V

I

V

A

G

R

R

V

A

G

G

V

T

G

G

T

V

D

D

S

N

I

V

D

D

H

L

D

E

A

A

K

T

E

R

F

K

G

G

V

I

G

L

I

V

S

M

V

N

T

T

P

P

G

N

M

G

N

N

A

S

E

L

T

S

V

A

A

A

D

E

Q

L

T

T

L

C

A

G

M

M

I

I

L

M

G

C

L

L

M

A

R

R

R

Q

V

I

V

P

T

Q

Q

A

D

T

Q

A

A

S

V

M

K

K

A

D

G

G

R

K

W

W

D

E

R

R

V

-

G

K

E

K

A

F

T

M

P

G

T

T

E

E

L

L

Y

N

R

G

K

K

T

T

V

L

G

G

L

I

I
x
L

G

G

A

L

G

G

I

R

I

I

G

G

K

R

A

E

V

V

I

A

R

T

R

R

L

M

L

Q

G

S

F

F

G

G

V

M

R

K

V

T

L

I

Y

G

F
x
Y

D
|
D

A
x
P

M
x
I

-
x
I

-

S

-

P

-

E

V

V

E

S

K

A

V

S

H

F

G

G

A

V

E

Q

R

Q

C

L

G

-

S

P

L

L

D

E

Q

E

L

I

L

W

G

P

S

L

S

C

D

D

I

F

V

I

S

T

L

V

H
|
H

A
x
T

P
|
P

L

L

A

P

D

S

T

T

R

T

E

G

L
|
L

M

L

N

N

A

D

A

N

R

T

I

F

A

A

L

Q

M

C

P

K

K

K

G

G

S

V

Y

R

L

V

I

V

N

N

T

C

S

A

R

R

G

G

L

I

V

V

Q

D

Q

E

P

G

A

A

V

L

F

L

A

R

A

A

L

L

R

Q

S

S

G

G

H

Q

L

C

A

A

G

G

A

A

L

L

D

D

V

V

F

F

E

T

V

E

E

E

P

P

G

R

A

D

E

R

A

A

L

L

A

V

D

D

V

H

P

E

N

N

L

V

I

I

A

S

S

C

A

P

H

H

I

L

G

G

G

A

I

S

S

T

T

K

E

E

S

A

I

Q

A

S

R

R

binding 4-[(1~{R})-1-[(2-methyl-5-phenyl-pyrazol-3-yl)carbonylamino]ethyl]benzoic acid: L145 (≠ I153), Y168 (≠ F176), D169 (= D177), P170 (≠ A178), I171 (≠ M179), I172 (vs. gap), H200 (= H205), T201 (≠ A206), P202 (= P207), L210 (= L215)

6rj2A Crystal structure of phgdh in complex with compound 40 (see paper)
35% identity, 80% coverage: 34:293/327 of query aligns to 24:286/299 of 6rj2A

query
sites
6rj2A

K

E

R

K

G

Q

N

N

R

L

T

S

A

K

D

E

E

E

L

L

A

I

T

A

L

E

L

L

E

Q

D

D

C

C

D

E

A

G

A

L

I

I

V

V

-

R

S

S

T

A

D

T

P

K

F

V

T

T

R

A

E

D

V

V

L

I

A

N

G

A

D

A

R

E

N

K

L

L

K

Q

V

V

I

V

A

G

R

R

V

A

G

G

V

T

G

G

T

V

D

D

S

N

I

V

D

D

H

L

D

E

A

A

K

T

E

R

F

K

G

G

V

I

G

L

I

V

S

M

V

N

T

T

P

P

G

N

M

G

N

N

A

S

E

L

T

S

V

A

A

A

D

E

Q

L

T

T

L

C

A

G

M

M

I

I

L

M

G

C

L

L

M

A

R

R

R

Q

V

I

V

P

T

Q

Q

A

D

T

Q

A

A

S

V

M

K

K

A

D

G

G

R

K

W

W

D

E

R

R

V

-

G

K

E

K

A

F

T

M

P

G

T

T

E

E

L

L

Y

N

R

G

K

K

T

T

V

L

G

G

L

I

I

L

G

G

A

L

G
|
G

I

R

I
|
I

G

G

K

R

A

E

V

V

I

A

R

T

R

R

L

M

L

Q

G

S

F

F

G

G

V

M

R

K

V

T

L

I

Y

G

F
x
Y

D
|
D

A
x
P

M
x
I

-
x
I

-

S

-

P

-

E

V

V

E

S

K

A

V

S

H

F

G

G

A

V

E

Q

R

Q

C

L

G

-

S

P

L

L

D

E

Q

E

L

I

L

W

G

P

S

L

S

C

D

D

I

F

V

I

S

T

L

V

H
|
H

A
x
T

P

P

L

L

A

P

D

S

T

T

R

T

E

G

L
|
L

M

L

N

N

A

D

A

N

R

T

I

F

A

A

L

Q

M

C

P

K

K

K

G

G

S

V

Y

R

L

V

I

V

N

N

T

C

S

A

R
|
R

G

G

L

I

V

V

Q

D

Q

E

P

G

A

A

V

L

F

L

A

R

A

A

L

L

R

Q

S

S

G

G

H

Q

L

C

A

A

G

G

A

A

L

L

D

D

V

V

F

F

E

T

V

E

E

E

P

P

G

R

A

D

E

R

A

A

L

L

A

V

D

D

V

H

P

E

N

N

L

V

I

I

A

S

S

C

A

P

H

H

I

L

G

G

G

A

I

S

S

T

T

K

E

E

S

A

I

Q

A

S

R

R

binding ~{N}-[(1~{R})-1-[4-(ethanoylsulfamoyl)phenyl]ethyl]-2-methyl-5-phenyl-pyrazole-3-carboxamide: G146 (= G156), I148 (= I158), Y166 (≠ F176), D167 (= D177), P168 (≠ A178), I169 (≠ M179), I170 (vs. gap), H198 (= H205), T199 (≠ A206), L208 (= L215), R228 (= R235)

7dkmA Phgdh covalently linked to oridonin (see paper)
35% identity, 80% coverage: 34:293/327 of query aligns to 28:290/306 of 7dkmA

query
sites
7dkmA

K

E

R

K

G

Q

N

N

R

L

T

S

A

K

D

E

E

E

L

L

A

I

T

A

L

E

L

L

E

Q

D

D

C

C

D

E

A

G

A

L

I

I

V

V

-

R

S

S

T

A

D

T

P

K

F

V

T

T

R

A

E

D

V

V

L

I

A

N

G

A

D

A

R

E

N

K

L

L

K

Q

V

V

I

V

A

G

R

R

V

A

G

G

V
x
T

G

G

T

V

D

D

S

N

I

V

D

D

H

L

D

E

A

A

K

T

E

R

F

K

G

G

V

I

G

L

I

V

S

M

V

N

T

T

P

P

G

N

M

G

N

N

A

S

E

L

T

S

V
x
A

A

A

D

E

Q

L

T

T

L

C

A

G

M

M

I

I

L

M

G

C

L

L

M

A

R

R

R

Q

V

I

V

P

T

Q

Q

A

D

T

Q

A

A

S

V

M

K

K

A

D

G

G

R

K

W

W

D

E

R

R

V

-

G

K

E

K

A

F

T

M

P

G

T

T

E

E

L

L

Y

N

R

G

K

K

T

T

V

L

G

G

L

I

I

L

G
|
G

A

L

G

G

I
x
R

I
|
I

G

G

K

R

A

E

V

V

I

A

R

T

R

R

L

M

L

Q

G

S

F

F

G

G

V

M

R

K

V

T

L

I

Y

G

F
x
Y

D
|
D

A
x
P

M
x
I

-

I

-

S

-

P

-

E

V

V

E

S

K

A

V

S

H

F

G

G

A

V

E

Q

R

Q

C

L

G

-

S

P

L

L

D

E

Q

E

L

I

L

W

G

P

S

L

S

C

D

D

I

F

V

I

S

T

L

V

H
|
H

A
x
T

P
|
P

L

L

A

P

D

S

T
|
T

R

T

E

G

L

L

M

L

N

N

A

D

A

N

R

T

I

F

A

A

L

Q

M

C

P

K

K

K

G

G

S

V

Y

R

L

V

I

V

N

N

T
x
C

S
x
A

R
|
R

G

G

L

I

V

V

Q

D

Q

E

P

G

A

A

V

L

F

L

A

R

A

A

L

L

R

Q

S

S

G

G

H

Q

L

C

A

A

G

G

A

A

L

L

D

D

V

V

F

F

E

T

V

E

E

E

P

P

G

R

A

D

E

R

A

A

L

L

A

V

D

D

V

H

P

E

N

N

L

V

I

I

A

S

S

C

A

P

H
|
H

I

L

G
|
G

G

A

I

S

S

T

T

K

E

E

S

A

I

Q

A

S

R

R

binding nicotinamide-adenine-dinucleotide: T74 (≠ V79), A102 (≠ V107), G148 (= G154), R151 (≠ I157), I152 (= I158), Y170 (≠ F176), D171 (= D177), P172 (≠ A178), I173 (≠ M179), H202 (= H205), T203 (≠ A206), P204 (= P207), T209 (= T212), C230 (≠ T233), A231 (≠ S234), R232 (= R235), H279 (= H282), G281 (= G284)

Sites not aligning to the query:

binding (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14R,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one: 14, 17, 18, 293

6cdfA Human ctbp1 (28-378) (see paper)
36% identity, 82% coverage: 52:320/327 of query aligns to 49:332/333 of 6cdfA

query
sites
6cdfA

A

A

A

L

I

M

V

Y

S

H

T

T

D

I

P

T

F

L

T

T

R

R

E

E

V

D

L

L

A

E

G

K

D

F

R

K

N

A

L

L

K

R

V

I

I

I

A

V

R

R

V

I

G

G

V

S

G

G

T

F

D

D

S

N

I

I

D

D

H

I

D

K

A

S

A

A

K

G

E

D

F

L

G

G

V

I

G

A

I

V

S

C

V

N

T

V

P

P

G

A

M

A

N

S

A

V

E

E

T

E

V
x
T

A

A

D

D

Q

S

T

T

L

L

A

C

M

H

I

I

L

L

G

N

L

L

M

Y

R

R

V

A

V

T

T

W

Q

L

D

H

Q

Q

A

A

V

L

K

R

A

E

G

G

-

T

R

R

W

V

D

Q

R

S

V

V

G

E

E

Q

-

I

-

R

-

E

-

V

-

A

A

S

T

G

P

A

T

A

E

R

L

I

Y

R

R

G

K

E

T

T

V

L

G

G

L

I

I

I

G
|
G

A

L

G

G

I
x
R

I
x
V

G

G

K

Q

A

A

V

V

I

A

R

L

R

R

L

A

L

K

G

A

F

F

G

G

V

F

R

N

V

V

L

L

Y

F

F
x
Y

D
|
D

A
x
P

M
x
Y

-

L

-

S

-

D

-

G

V

V

E

E

K

R

V

A

H

L

G

G

A

L

E

Q

R

R

C

V

G

S

S

T

L

L

D

Q

Q

D

L

L

G

F

S

H

S

S

D

D

I

C

V

V

S

T

L

L

H
|
H

A
x
C

P

G

L

L

L

N

A

E

D

H

T
x
N

R

H

E

H

L

L

M

I

N

N

A

D

A

F

R

T

I

V

A

K

L

Q

M

M

P

R

K

Q

G

G

S

A

Y

F

L

L

I

V

N

N

T
|
T

S
x
A

R
|
R

G

G

L

L

V

V

Q

D

Q

E

P

K

A

A

V

L

F

A

A

Q

A

A

L

L

R

K

S

E

G

G

H

R

L

I

A

R

G

G

A

A

L

L

D

D

V

V

F

H

E

E

V

S

E

E

P

P

P

F

G

S

A

F

E

S

A

Q

-

G

-

P

L

L

A

K

D

D

V

A

P

P

N

N

L

L

I

I

A

C

S

T

A

P

H
|
H

I

A

G

A

G
x
W

I

Y

S

S

T

E

E

Q

S

A

I

S

A

I

R

E

M

M

T

R

R

E

S

E

A

A

T

A

T

R

S

E

V

I

L

R

S

R

V

A

L

I

N

T

G

G

E

R

I

I

P

P

D

D

T

S

V

L

-

K

-

N

-

C

I

V

N

N

P

K

D

D

A

H

L

L

binding 1,4-dihydronicotinamide adenine dinucleotide: T104 (≠ V107), G157 (= G154), R160 (≠ I157), V161 (≠ I158), Y179 (≠ F176), D180 (= D177), P181 (≠ A178), Y182 (≠ M179), H212 (= H205), C213 (≠ A206), N219 (≠ T212), T240 (= T233), A241 (≠ S234), R242 (= R235), H291 (= H282), W294 (≠ G285)

6v89A Human ctbp1 (28-375) in complex with amp (see paper)
36% identity, 82% coverage: 52:320/327 of query aligns to 48:331/332 of 6v89A

query
sites
6v89A

A

A

A

L

I

M

V

Y

S

H

T

T

D

I

P

T

F

L

T

T

R

R

E

E

V

D

L

L

A

E

G

K

D

F

R

K

N

A

L

L

K

R

V

I

I

I

A

V

R

R

V

I

G

G

V

S

G

G

T

F

D

D

S

N

I

I

D

D

H

I

D

K

A

S

A

A

K

G

E

D

F

L

G

G

V

I

G

A

I

V

S

C

V

N

T

V

P

P

G

A

M

A

N

S

A

V

E

E

T

E

V

T

A

A

D

D

Q

S

T

T

L

L

A

C

M

H

I

I

L

L

G

N

L

L

M

Y

R

R

V

A

V

T

T

W

Q

L

D

H

Q

Q

A

A

V

L

K

R

A

E

G

G

-

T

R

R

W

V

D

Q

R

S

V

V

G

E

E

Q

-

I

-

R

-

E

-

V

-

A

A

S

T

G

P

A

T

A

E

R

L

I

Y

R

R

G

K

E

T

T

V

L

G

G

L

I

I

I

G
|
G

A

L

G
|
G

I
x
R

I

V

G

G

K

Q

A

A

V

V

I

A

R

L

R

R

L

A

L

K

G

A

F

F

G

G

V

F

R

N

V

V

L

L

Y

F

F
x
Y

D
|
D

A
x
P

M
x
Y

-

L

-

S

-

D

-

G

V

V

E

E

K

R

V

A

H

L

G

G

A

L

E

Q

R

R

C

V

G

S

S

T

L

L

D

Q

Q

D

L

L

G

F

S

H

S

S

D

D

I

C

V

V

S

T

L

L

H

H

A
x
C

P

G

L

L

L

N

A

E

D

H

T
x
N

R

H

E

H

L

L

M

I

N

N

A

D

A

F

R

T

I

V

A

K

L

Q

M

M

P

R

K

Q

G

G

S

A

Y

F

L

L

I

V

N

N

T

T

S

A

R

R

G

G

L

L

V

V

Q

D

Q

E

P

K

A

A

V

L

F

A

A

Q

A

A

L

L

R

K

S

E

G

G

H

R

L

I

A

R

G

G

A

A

L

L

D

D

V

V

F

H

E

E

V

S

E

E

P

P

P

F

G

S

A

F

E

S

A

Q

-

G

-

P

L

L

A

K

D

D

V

A

P

P

N

N

L

L

I

I

A

C

S

T

A

P

H

H

I

A

G

A

G

W

I

Y

S

S

T

E

E

Q

S

A

I

S

A

I

R

E

M

M

T

R

R

E

S

E

A

A

T

A

T

R

S

E

V

I

L

R

S

R

V

A

L

I

N

T

G

G

E

R

I

I

P

P

D

D

T

S

V

L

-

K

-

N

-

C

I

V

N

N

P

K

D

D

A

H

L

L

binding adenosine monophosphate: G156 (= G154), G158 (= G156), R159 (≠ I157), Y178 (≠ F176), D179 (= D177), P180 (≠ A178), Y181 (≠ M179), C212 (≠ A206), N218 (≠ T212)

4u6sA Ctbp1 in complex with substrate phenylpyruvate (see paper)
36% identity, 81% coverage: 52:316/327 of query aligns to 48:324/328 of 4u6sA

query
sites
4u6sA

A

A

A

L

I

M

V
x
Y

S
x
H

T

T

D

I

P

T

F

L

T

T

R

R

E

E

V

D

L

L

A

E

G

K

D

F

R

K

N

A

L

L

K

R

V

I

I

I

A

V

R

R

V
x
I

G
|
G

V
x
S

G
|
G

T

F

D

D

S

N

I

I

D

D

H

I

D

K

A

S

A

A

K

G

E

D

F

L

G

G

V

I

G

A

I

V

S

C

V

N

T

V

P

P

G

A

M

A

N
x
S

A

V

E

E

T

E

V
x
T

A

A

D

D

Q

S

T

T

L

L

A

C

M

H

I

I

L

L

G

N

L

L

M

Y

R

R

V

A

V

T

T

W

Q

L

D

H

Q

Q

A

A

V

L

K

R

A

E

G

G

-

T

R

R

W

V

D

Q

R

S

V

V

G

E

E

Q

-

I

-

R

-

E

-

V

-

A

A

S

T

G

P

A

T

A

E

R

L

I

Y

R

R

G

K

E

T

T

V

L

G

G

L

I

I

I

G
|
G

A

L

G
|
G

I
x
R

I
x
V

G

G

K

Q

A

A

V

V

I

A

R

L

R

R

L

A

L

K

G

A

F

F

G

G

V

F

R

N

V

V

L

L

Y

F

F
x
Y

D
|
D

A
x
P

M
x
Y

-

L

-

S

-

D

-

G

V

V

E

E

K

R

V

A

H

L

G

G

A

L

E

Q

R

R

C

V

G

S

S

T

L

L

D

Q

Q

D

L

L

G

F

S

H

S

S

D

D

I

C

V

V

S

T

L

L

H
|
H

A
x
C

P
x
G

L

L

L

N

A

E

D

H

T
x
N

R

H

E

H

L

L

M

I

N

N

A

D

A

F

R

T

I

V

A

K

L

Q

M

M

P

R

K

Q

G

G

S

A

Y

F

L

L

I

V

N

N

T
|
T

S
x
A

R
|
R

G

G

L

L

V

V

Q

D

Q

E

P

K

A

A

V

L

F

A

A

Q

A

A

L

L

R

K

S

E

G

G

H

R

L

I

A

R

G

G

A

A

L

L

D
|
D

V

V

F

H

E

E

V

S

E
|
E

P

P

P

F

G

S

A

F

E

S

A

Q

-

G

-

P

L

L

A

K

D

D

V

A

P

P

N

N

L

L

I

I

A

C

S

T

A

P

H
|
H

I

A

G

A

G
x
W

I

Y

S

S

T

E

E

Q

S

A

I

S

A

I

R

E

M
|
M

T

R

R

E

S

E

A

A

T

A

T

R

S

E

V

I

L

R

S

R

V

A

L

I

N

T

G

G

E

R

I

I

P

P

D

D

T

S

V

L

I

K

N

N

active site: S99 (≠ N103), R241 (= R235), D265 (= D259), E270 (= E264), H290 (= H282)
binding nicotinamide-adenine-dinucleotide: T103 (≠ V107), G156 (= G154), G158 (= G156), R159 (≠ I157), V160 (≠ I158), Y178 (≠ F176), D179 (= D177), P180 (≠ A178), Y181 (≠ M179), H211 (= H205), C212 (≠ A206), G213 (≠ P207), N218 (≠ T212), T239 (= T233), A240 (≠ S234), R241 (= R235), H290 (= H282), W293 (≠ G285)
binding 3-phenylpyruvic acid: Y51 (≠ V55), H52 (≠ S56), I73 (≠ V77), G74 (= G78), S75 (≠ V79), G76 (= G80), R241 (= R235), W293 (≠ G285), M302 (= M294)

4u6qA Ctbp1 bound to inhibitor 2-(hydroxyimino)-3-phenylpropanoic acid (see paper)
36% identity, 81% coverage: 52:316/327 of query aligns to 48:324/328 of 4u6qA

query
sites
4u6qA

A

A

A

L

I

M

V
x
Y

S

H

T

T

D

I

P

T

F

L

T

T

R

R

E

E

V

D

L

L

A

E

G

K

D

F

R

K

N

A

L

L

K

R

V

I

I

I

A

V

R

R

V
x
I

G
|
G

V
x
S

G
|
G

T

F

D

D

S

N

I

I

D

D

H

I

D

K

A

S

A

A

K

G

E

D

F

L

G

G

V

I

G

A

I

V

S

C

V

N

T

V

P

P

G

A

M

A

N
x
S

A

V

E

E

T

E

V
x
T

A

A

D

D

Q

S

T

T

L

L

A

C

M

H

I

I

L

L

G

N

L

L

M

Y

R

R

V

A

V

T

T

W

Q

L

D

H

Q

Q

A

A

V

L

K

R

A

E

G

G

-

T

R

R

W

V

D

Q

R

S

V

V

G

E

E

Q

-

I

-

R

-

E

-

V

-

A

A

S

T

G

P

A

T

A

E

R

L

I

Y

R

R

G

K

E

T

T

V

L

G

G

L

I

I

I

G
|
G

A

L

G

G

I
x
R

I
x
V

G

G

K

Q

A

A

V

V

I

A

R

L

R

R

L

A

L

K

G

A

F

F

G

G

V

F

R

N

V

V

L

L

Y

F

F
x
Y

D
|
D

A
x
P

M
x
Y

-

L

-

S

-

D

-

G

V

V

E

E

K

R

V

A

H

L

G

G

A

L

E

Q

R

R

C

V

G

S

S

T

L

L

D

Q

Q

D

L

L

G

F

S

H

S

S

D

D

I

C

V

V

S

T

L

L

H
|
H

A
x
C

P
x
G

L

L

L

N

A

E

D

H

T
x
N

R

H

E

H

L

L

M

I

N

N

A

D

A

F

R

T

I

V

A

K

L

Q

M

M

P

R

K

Q

G

G

S

A

Y

F

L

L

I

V

N

N

T
|
T

S
x
A

R
|
R

G

G

L

L

V

V

Q

D

Q

E

P

K

A

A

V

L

F

A

A

Q

A

A

L

L

R

K

S

E

G

G

H

R

L

I

A

R

G

G

A

A

L

L

D
|
D

V

V

F

H

E

E

V

S

E
|
E

P

P

P

F

G

S

A

F

E

S

A

Q

-

G

-

P

L

L

A

K

D

D

V

A

P

P

N

N

L

L

I

I

A

C

S

T

A

P

H
|
H

I

A

G

A

G
x
W

I

Y

S

S

T

E

E

Q

S

A

I

S

A

I

R

E

M
|
M

T

R

R

E

S

E

A

A

T

A

T

R

S

E

V

I

L

R

S

R

V

A

L

I

N

T

G

G

E

R

I

I

P

P

D

D

T

S

V

L

I

K

N

N

active site: S99 (≠ N103), R241 (= R235), D265 (= D259), E270 (= E264), H290 (= H282)
binding (2E)-2-(hydroxyimino)-3-phenylpropanoic acid: Y51 (≠ V55), I73 (≠ V77), G74 (= G78), S75 (≠ V79), G76 (= G80), R241 (= R235), H290 (= H282), W293 (≠ G285), M302 (= M294)
binding 1,4-dihydronicotinamide adenine dinucleotide: S75 (≠ V79), T103 (≠ V107), G156 (= G154), R159 (≠ I157), V160 (≠ I158), Y178 (≠ F176), D179 (= D177), P180 (≠ A178), Y181 (≠ M179), H211 (= H205), C212 (≠ A206), G213 (≠ P207), N218 (≠ T212), T239 (= T233), A240 (≠ S234), R241 (= R235), H290 (= H282), W293 (≠ G285)

4lceA Ctbp1 in complex with substrate mtob (see paper)
36% identity, 81% coverage: 52:316/327 of query aligns to 47:323/327 of 4lceA

query
sites
4lceA

A

A

A

L

I

M

V

Y

S

H

T

T

D

I

P

T

F

L

T

T

R

R

E

E

V

D

L

L

A

E

G

K

D

F

R

K

N

A

L

L

K

R

V

I

I

I

A

V

R
|
R

V

I

G
|
G

V
x
S

G
|
G

T

F

D

D

S

N

I

I

D

D

H

I

D

K

A

S

A

A

K

G

E

D

F

L

G

G

V

I

G

A

I

V

S

C

V

N

T

V

P

P

G

A

M

A

N
x
S

A

V

E

E

T

E

V
x
T

A

A

D

D

Q

S

T

T

L

L

A

C

M

H

I

I

L

L

G

N

L

L

M

Y

R

R

V

A

V

T

T

W

Q

L

D

H

Q

Q

A

A

V

L

K

R

A

E

G

G

-

T

R

R

W

V

D

Q

R

S

V

V

G

E

E

Q

-

I

-

R

-

E

-

V

-

A

A

S

T

G

P

A

T

A

E

R

L

I

Y

R

R

G

K

E

T

T

V

L

G

G

L

I

I

I

G
|
G

A

L

G
|
G

I
x
R

I
x
V

G

G

K

Q

A

A

V

V

I

A

R

L

R

R

L

A

L

K

G

A

F

F

G

G

V

F

R

N

V

V

L

L

Y

F

F
x
Y

D
|
D

A
x
P

M
x
Y

-

L

-

S

-

D

-

G

V

V

E

E

K

R

V

A

H

L

G

G

A

L

E

Q

R

R

C

V

G

S

S

T

L

L

D

Q

Q

D

L

L

G

F

S

H

S

S

D

D

I

C

V

V

S

T

L

L

H
|
H

A
x
C

P

G

L

L

L
x
N

A

E

D

H

T
x
N

R

H

E

H

L

L

M

I

N

N

A

D

A

F

R

T

I

V

A

K

L

Q

M

M

P

R

K

Q

G

G

S

A

Y

F

L

L

I

V

N

N

T
|
T

S
x
A

R
|
R

G

G

L

L

V

V

Q

D

Q

E

P

K

A

A

V

L

F

A

A

Q

A

A

L

L

R

K

S

E

G

G

H

R

L

I

A

R

G

G

A

A

L

L

D
|
D

V

V

F

H

E

E

V

S

E
|
E

P

P

P

F

G

S

A

F

E

S

A

Q

-

G

-

P

L

L

A

K

D

D

V

A

P

P

N

N

L

L

I

I

A

C

S

T

A

P

H
|
H

I

A

G

A

G
x
W

I

Y

S

S

T

E

E

Q

S

A

I

S

A

I

R

E

M

M

T

R

R

E

S

E

A

A

T

A

T

R

S

E

V

I

L

R

S

R

V

A

L

I

N

T

G

G

E

R

I

I

P

P

D

D

T

S

V

L

I

K

N

N

active site: S98 (≠ N103), R240 (= R235), D264 (= D259), E269 (= E264), H289 (= H282)
binding 4-(methylsulfanyl)-2-oxobutanoic acid: R71 (= R76), G73 (= G78), S74 (≠ V79), G75 (= G80), R240 (= R235), H289 (= H282), W292 (≠ G285)
binding nicotinamide-adenine-dinucleotide: S74 (≠ V79), T102 (≠ V107), G155 (= G154), G157 (= G156), R158 (≠ I157), V159 (≠ I158), Y177 (≠ F176), D178 (= D177), P179 (≠ A178), Y180 (≠ M179), H210 (= H205), C211 (≠ A206), N214 (≠ L209), N217 (≠ T212), T238 (= T233), A239 (≠ S234), R240 (= R235), W292 (≠ G285)

1hl3A Ctbp/bars in ternary complex with NAD(h) and pidlskk peptide (see paper)
35% identity, 81% coverage: 52:316/327 of query aligns to 48:324/331 of 1hl3A

query
sites
1hl3A

A

A

A

L

I

M

V

Y

S

H

T

T

D

I

P

T

F

L

T

T

R

R

E

E

V

D

L

L

A

E

G

K

D

F

R

K

N

A

L

L

K

R

V

I

I

I

A

V

R

R

V

I

G

G

V

S

G

G

T

F

D

D

S

N

I

I

D

D

H

I

D

K

A

S

A

A

K

G

E

D

F

L

G

G

V

I

G

A

I

V

S

C

V

N

T

V

P

P

G

A

M

A

N
x
S

A

V

E

E

T

E

V
x
T

A

A

D

D

Q

S

T

T

L

L

A

C

M

H

I

I

L

L

G

N

L

L

M

Y

R

R

V

T

T

W

Q

L

D

H

Q

Q

A

A

V

L

K

R

A

E

G

G

-

T

R

R

W

V

D

Q

R

S

V

V

G

E

E

Q

-

I

-

R

-

E

-

V

-

A

A

S

T

G

P

A

T

A

E

R

L

I

Y

R

R

G

K

E

T

T

V

L

G

G

L

I

I

I

G

G

A

L

G
|
G

I
x
R

I
x
V

G

G

K

Q

A

A

V

V

I

A

R

L

R

R

L

A

L

K

G

A

F

F

G

G

V

F

R

N

V

V

L

L

Y

F

F

Y

D
|
D

A

P

M
x
Y

-

L

-

S

-

D

-

G

V

I

E

E

K

R

V

A

H

L

G

G

A

L

E

Q

R

R

C

V

G

S

S

T

L

L

D

Q

Q

D

L

L

G

F

S

H

S

S

D

D

I

C

V

V

S

T

L

L

H
|
H

A
x
C

P
x
G

L

L

L

N

A

E

D

H

T
x
N

R

H

E

H

L

L

M

I

N

N

A

D

A

F

R

T

I

V

A

K

L

Q

M

M

P

R

K

Q

G

G

S

A

Y

F

L

L

I

V

N

N

T
|
T

S
x
A

R
|
R

G

G

L

L

V

V

Q

D

Q

E

P

K

A

A

V

L

F

A

A

Q

A

A

L

L

R

K

S

E

G

G

H

R

L

I

A

R

G

G

A

A

L

L

D
|
D

V

V

F

H

E

E

V

S

E
|
E

P

P

P

F

G

S

A

F

E

S

A

Q

-

G

-

P

L

L

A

K

D

D

V

A

P

P

N

N

L

L

I

I

A

C

S

T

A

P

H
|
H

I

A

G

A

G

W

I

Y

S

S

T

E

E

Q

S

A

I

S

A

I

R

E

M

M

T

R

R

E

S

E

A

A

T

A

T

R

S

E

V

I

L

R

S

R

V

A

L

I

N

T

G

G

E

R

I

I

P

P

D

D

T

S

V

L

I

K

N

N

active site: S99 (≠ N103), R241 (= R235), D265 (= D259), E270 (= E264), H290 (= H282)
binding nicotinamide-adenine-dinucleotide: T103 (≠ V107), G158 (= G156), R159 (≠ I157), V160 (≠ I158), D179 (= D177), Y181 (≠ M179), H211 (= H205), C212 (≠ A206), G213 (≠ P207), N218 (≠ T212), T239 (= T233), A240 (≠ S234), R241 (= R235), D265 (= D259), H290 (= H282)

Sites not aligning to the query:

binding : 17, 26, 27, 28, 29, 36, 38, 39, 331

1hkuA Ctbp/bars: a dual-function protein involved in transcription corepression and golgi membrane fission (see paper)
35% identity, 81% coverage: 52:316/327 of query aligns to 48:324/331 of 1hkuA

query
sites
1hkuA

A

A

A

L

I

M

V

Y

S

H

T

T

D

I

P

T

F

L

T

T

R

R

E

E

V

D

L

L

A

E

G

K

D

F

R

K

N

A

L

L

K

R

V

I

I

I

A

V

R

R

V

I

G

G

V
x
S

G

G

T

F

D

D

S

N

I

I

D

D

H

I

D

K

A

S

A

A

K

G

E

D

F

L

G

G

V

I

G

A

I

V

S

C

V

N

T

V

P

P

G

A

M

A

N
x
S

A

V

E

E

T

E

V
x
T

A

A

D

D

Q

S

T

T

L

L

A

C

M

H

I

I

L

L

G

N

L

L

M

Y

R

R

V

T

T

W

Q

L

D

H

Q

Q

A

A

V

L

K

R

A

E

G

G

-

T

R

R

W

V

D

Q

R

S

V

V

G

E

E

Q

-

I

-

R

-

E

-

V

-

A

A

S

T

G

P

A

T

A

E

R

L

I

Y

R

R

G

K

E

T

T

V

L

G

G

L

I

I

I

G
|
G

A

L

G
|
G

I
x
R

I
x
V

G

G

K

Q

A

A

V

V

I

A

R

L

R

R

L

A

L

K

G

A

F

F

G

G

V

F

R

N

V

V

L

L

Y

F

F
x
Y

D
|
D

A
x
P

M
x
Y

-

L

-

S

-

D

-

G

V

I

E

E

K

R

V

A

H

L

G

G

A

L

E

Q

R

R

C

V

G

S

S

T

L

L

D

Q

Q

D

L

L

G

F

S

H

S

S

D

D

I

C

V

V

S

T

L

L

H

H

A
x
C

P

G

L

L

L

N

A

E

D

H

T
x
N

R

H

E

H

L

L

M

I

N

N

A

D

A

F

R

T

I

V

A

K

L

Q

M

M

P

R

K

Q

G

G

S

A

Y

F

L

L

I

V

N

N

T
|
T

S
x
A

R
|
R

G

G

L

L

V

V

Q

D

Q

E

P

K

A

A

V

L

F

A

A

Q

A

A

L

L

R

K

S

E

G

G

H

R

L

I

A

R

G

G

A

A

L

L

D
|
D

V

V

F

H

E

E

V

S

E
|
E

P

P

P

F

G

S

A

F

E

S

A

Q

-

G

-

P

L

L

A

K

D

D

V

A

P

P

N

N

L

L

I

I

A

C

S

T

A

P

H
|
H

I

A

G

A

G
x
W

I

Y

S

S

T

E

E

Q

S

A

I

S

A

I

R

E

M

M

T

R

R

E

S

E

A

A

T

A

T

R

S

E

V

I

L

R

S

R

V

A

L

I

N

T

G

G

E

R

I

I

P

P

D

D

T

S

V

L

I

K

N

N

active site: S99 (≠ N103), R241 (= R235), D265 (= D259), E270 (= E264), H290 (= H282)
binding nicotinamide-adenine-dinucleotide: S75 (≠ V79), T103 (≠ V107), G156 (= G154), G158 (= G156), R159 (≠ I157), V160 (≠ I158), Y178 (≠ F176), D179 (= D177), P180 (≠ A178), Y181 (≠ M179), C212 (≠ A206), N218 (≠ T212), T239 (= T233), A240 (≠ S234), R241 (= R235), H290 (= H282), W293 (≠ G285)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 331

Q13363 C-terminal-binding protein 1; CtBP1; EC 1.1.1.- from Homo sapiens (Human) (see 4 papers)
36% identity, 83% coverage: 46:316/327 of query aligns to 67:349/440 of Q13363

query
sites
Q13363

L

L

L

N

E

E

D

A

C

V

D

G

A

A

A

L

I

M

V

Y

S

H

T

T

D

I

P

T

F

L

T

T

R

R

E

E

V

D

L

L

A

E

G

K

D

F

R

K

N

A

L

L

K

R

V

I

I

I

A

V

R

R

V

I

G

G

V

S

G

G

T

F

D

D

S

N

I

I

D

D

H

I

D

K

A

S

A

A

K

G

E

D

F

L

G

G

V

I

G

A

I

V

S

C

V

N

T

V

P

P

G

A

M

A

N

S

A

V

E

E

T

E

V

T

A

A

D

D

Q

S

T

T

L

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x
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N

N

C134 (≠ A113) mutation to A: Strongly reduces E1A binding; when associated with A-138; A-141 and A-150.
N138 (≠ G117) mutation to A: Strongly reduces E1A binding; when associated with A-134; A-141 and A-150.
R141 (= R120) mutation to A: Strongly reduces E1A binding; when associated with A-134; A-138 and A-150.
RR 141:142 (= RR 120:121) mutation to AA: Strongly reduces E1A binding; when associated with A-163 and A-171.
L150 (≠ V129) mutation to A: Strongly reduces E1A binding; when associated with A-134; A-138 and A-141.
R163 (vs. gap) mutation to A: Strongly reduces E1A binding; when associated with A-141; A-142 and A-171.
R171 (≠ E144) mutation to A: Strongly reduces E1A binding; when associated with A-141; A-142 and A-163.
G181 (= G154) mutation to V: Strongly reduces E1A binding; when associated with V-183 and A-204.
G183 (= G156) mutation to A: Reduced proteolytic processing mediated by CAPN3; when associated with A-186.; mutation to V: Strongly reduces E1A binding; when associated with V-181 and A-204.
G186 (= G159) mutation to A: Reduced proteolytic processing mediated by CAPN3; when associated with A-183.
D204 (= D177) mutation to A: Strongly reduces E1A binding; when associated with V-181 and V-183.; mutation to L: Reduced proteolytic processing mediated by CAPN3.
R266 (= R235) mutation to A: Strongly reduces E1A binding; when associated with A-290; A-295 and A-315.
D290 (= D259) mutation to A: Strongly reduces E1A binding; when associated with A-266; A-295 and A-315.
E295 (= E264) mutation to A: Strongly reduces E1A binding; when associated with A-266; A-290 and A-315.
H315 (= H282) mutation to A: Strongly reduces E1A binding; when associated with A-266; A-290 and A-295.

Sites not aligning to the query:

52 A→E: Loss of interaction with SIMC1. No effect on its proteolytic processing mediated by CAPN3.
66 V→R: Loss of interaction with SIMC1. Reduced proteolytic processing mediated by CAPN3.
375:376 Cleavage; by CAPN1
387:388 Cleavage; by CAPN1
409:410 Cleavage; by CAPN1 and CAPN3
422 modified: Phosphoserine; by HIPK2; S→A: Abolishes phosphorylation by HIPK2 and prevents UV-induced clearance.
428 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)

Q9Z2F5 C-terminal-binding protein 1; CtBP1; 50 kDa BFA-dependent ADP-ribosylation substrate; BARS-50; C-terminal-binding protein 3; CtBP3; EC 1.1.1.- from Rattus norvegicus (Rat) (see 3 papers)
35% identity, 83% coverage: 46:316/327 of query aligns to 56:338/430 of Q9Z2F5

query
sites
Q9Z2F5

L

L

L

N

E

E

D

A

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V

D

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A

A

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T

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x
S

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R

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N

S89 (≠ V79) binding NAD(+)
IGLGRV 169:174 (≠ IGAGII 153:158) binding NAD(+)
G172 (= G156) mutation to E: Loss dimerization and of NAD binding.
D193 (= D177) binding NAD(+)
226:232 (vs. 206:212, 14% identical) binding NAD(+)
TAR 253:255 (≠ TSR 233:235) binding NAD(+)
D279 (= D259) binding NAD(+)

Sites not aligning to the query:

41 A→E: Strongly reduces interaction with E1A.
55 V→R: Strongly reduces interaction with E1A.

Query Sequence

>WP_012706517.1 NCBI__GCF_000018545.1:WP_012706517.1
MARVFISWPGYSPRDEDTGARLIAAGHELVLRPKRGNRTADELATLLEDCDAAIVSTDPF
TREVLAGDRNLKVIARVGVGTDSIDHDAAKEFGVGISVTPGMNAETVADQTLAMILGLMR
RVVTQDQAVKAGRWDRVGEATPTELYRKTVGLIGAGIIGKAVIRRLLGFGVRVLYFDAMV
EKVHGAERCGSLDQLLGSSDIVSLHAPLLADTRELMNAARIALMPKGSYLINTSRGGLVQ
QPAVFAALRSGHLAGAALDVFEVEPPGAEALADVPNLIASAHIGGISTESIARMTRSATT
SVLSVLNGEIPDTVINPDALRERLAGA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory