SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012706657.1 NCBI__GCF_000018545.1:WP_012706657.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 5 hits to proteins with known functional sites (download)

3tdtA Complex of tetrahydrodipicolinate n-succinyltransferase with 2-amino- 6-oxopimelate and coenzyme a (see paper)
61% identity, 98% coverage: 6:284/285 of query aligns to 1:271/274 of 3tdtA

query
sites
3tdtA

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active site: D141 (= D148), G166 (= G173), E189 (= E196)
binding 2-amino-6-oxopimelic acid: R104 (= R111), M124 (= M131), M139 (= M146), D141 (= D148)
binding coenzyme a: G185 (= G192), A186 (= A193), S203 (≠ G210), M204 (= M211), R217 (= R224), V234 (= V241), K254 (= K267), K259 (= K272), T260 (= T273), K263 (= K276)

2tdtA Complex of tetrahydrodipicolinate n-succinyltransferase with 2- aminopimelate and coenzyme a (see paper)
61% identity, 98% coverage: 6:284/285 of query aligns to 1:271/274 of 2tdtA

query
sites
2tdtA

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active site: D141 (= D148), G166 (= G173), E189 (= E196)
binding coenzyme a: S159 (= S166), F183 (= F190), G185 (= G192), S203 (≠ G210), M204 (= M211), R217 (= R224), K254 (= K267), K259 (= K272), T260 (= T273), K263 (= K276)
binding (2S)-2-aminoheptanedioic acid: R104 (= R111), M124 (= M131), M139 (= M146), D141 (= D148)

1kgtA Crystal structure of tetrahydrodipicolinate n-succinyltransferase in complex with pimelate and succinyl-coa (see paper)
61% identity, 98% coverage: 6:284/285 of query aligns to 1:271/274 of 1kgtA

query
sites
1kgtA

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active site: D141 (= D148), G166 (= G173), E189 (= E196)
binding pimelic acid: R104 (= R111), M124 (= M131), M139 (= M146)
binding succinyl-coenzyme a: D141 (= D148), S159 (= S166), A186 (= A193), S203 (≠ G210), R217 (= R224), K259 (= K272), T260 (= T273), K263 (= K276)

1kgqA Crystal structure of tetrahydrodipicolinate n-succinyltransferase in complex with l-2-aminopimelate and succinamide-coa (see paper)
61% identity, 98% coverage: 6:284/285 of query aligns to 1:271/274 of 1kgqA

query
sites
1kgqA

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active site: D141 (= D148), G166 (= G173), E189 (= E196)
binding (2S)-2-aminoheptanedioic acid: R104 (= R111), M124 (= M131), M139 (= M146), D141 (= D148)
binding succinamide-coa: F183 (= F190), G185 (= G192), S203 (≠ G210), M204 (= M211), R217 (= R224), V234 (= V241), K254 (= K267), K259 (= K272), T260 (= T273), K263 (= K276)

3r8yA Structure of the bacillus anthracis tetrahydropicolinate succinyltransferase
42% identity, 32% coverage: 111:201/285 of query aligns to 77:168/203 of 3r8yA

query
sites
3r8yA

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x
G

C

R

A

A

Q

T

I

V

G

G

K

K

N

N

V

C

H

H

L

V

S

G

G

A

G

G

V

A

G

V

I

L

G

A

G
|
G

V

V

L

I

E

E

P

P

M

P

Q

S

A

A

G

K

P

P

T

V

I

I

I

V

E

E

D

D

N

D

C

V

F

V

I

I

G

G

A

A

R

N

S

V

E
x
V

V

V

V

L

E

E

G

G

C

V

active site: D115 (= D148), G140 (= G173), V163 (≠ E196)
binding calcium ion: G121 (= G154), G122 (≠ S155)

Query Sequence

>WP_012706657.1 NCBI__GCF_000018545.1:WP_012706657.1
MTNHDLASLSQTIETAFEDREAVSTSTRGAIRDAVEAALNLLDSGKVRVAERGADGTWTV
NQWLKKAVLLSFRLNPMELVKGGPGESVWWDKVASKFDGWSVNEFEKAGFRAVPNCVVRR
SAYIAPNAILMPSFVNLGAYVGEGTMVDTWATVGSCAQIGKNVHLSGGVGIGGVLEPMQA
GPTIIEDNCFIGARSEVVEGCIVREGSVLGMGVFIGKSTKIVDRATGEVTYGEVPPYSVV
VAGSMPSGSTMANGQPAPNLYCAVIVKRVDEKTRSKTGINELLRD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory