SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012706904.1 NCBI__GCF_000018545.1:WP_012706904.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P78061 Gamma-glutamylputrescine synthetase PuuA; Gamma-Glu-Put synthetase; Glutamate--putrescine ligase; EC 6.3.1.11 from Escherichia coli (strain K12) (see paper)
34% identity, 90% coverage: 48:477/478 of query aligns to 40:471/472 of P78061

query
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P78061

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H282 (= H288) mutation to N: Activity is impaired to 9% of wild-type.
R357 (= R364) mutation to Q: Activity is impaired to 3% of wild-type.

8tfkA Glutamine synthetase (see paper)
32% identity, 77% coverage: 103:470/478 of query aligns to 62:432/440 of 8tfkA

query
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binding adenosine-5'-diphosphate: E128 (= E167), D194 (≠ N241), F195 (≠ L242), F197 (≠ H244), N243 (≠ H290), R312 (= R359), R317 (= R364), G325 (≠ A372), R327 (= R374)
binding magnesium ion: E128 (= E167), E128 (= E167), E130 (= E169), E185 (= E232), E192 (= E239), E192 (= E239), H241 (= H288), E329 (= E376)
binding l-methionine-s-sulfoximine phosphate: E128 (= E167), E130 (= E169), E185 (= E232), E192 (= E239), G237 (= G284), H241 (= H288), R294 (= R341), E300 (≠ S347), R312 (= R359), R331 (= R378)

8oooA Glutamine synthetase from methanothermococcus thermolithotrophicus in complex with 2-oxoglutarate and mgatp at 2.15 a resolution (see paper)
32% identity, 77% coverage: 102:470/478 of query aligns to 68:440/447 of 8oooA

query
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binding 2-oxoglutaric acid: R87 (≠ E121), V93 (≠ T125), P170 (≠ D209), R173 (≠ I212), R174 (≠ D213), S190 (≠ L229)
binding adenosine-5'-triphosphate: E136 (= E167), E188 (≠ D227), F203 (≠ L242), K204 (≠ R243), F205 (≠ H244), H251 (= H290), S253 (= S292), R325 (= R364), R335 (= R374)

Sites not aligning to the query:

binding 2-oxoglutaric acid: 17, 19, 33

8ooqB Glutamine synthetase from Methanothermococcus thermolithotrophicus (see paper)
32% identity, 77% coverage: 102:470/478 of query aligns to 67:439/446 of 8ooqB

query
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8ooqB

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I

V

V

L

P

P

W

W

E
x
R

-

P

-

S

S

E

D

K

P

S

T
x
V

A

A

Q

R

V

V

I

I

C

C

D

D

I

V

V

Y

G

T

S

T

Q

K

G

G

E

K

Q

P

I

F

S

E

Y

G

T

D

P

P

R

R

N

G

V

C

L

L

K

K

R

R

V

V

L

M

D

E

L

E

Y

F

R

K

Q

K

K

E

-

F

G

N

W

G

K

E

P

Y

V

F

V

V

A

G

P

P

E

E

I

P

E
|
E

F

F

Y

F

L

L

V

L

A

K

Q

K

N

-

D

-

D

D

P

P

D

H

Y

N

P

P

L

H

R

K

P

Y

P

I

K

P

G

A

R

D

S

D

G

G

R

-

S

-

I

-

L

-

G

-

Q

-

G

G

Y

Y

-

F

S

D

I

L

A

E

G

P

I

M

N

D

E

E

F

A

D
x
P

E

D

L

I

I
x
R

D
x
R

D

D

I

I

Y

V

H

F

F

A

S

L

E

E

K

N

Q

L

G

G

L

F

E

H

I

V

D

E

T

A

L
x
S

I

H

H

H

E
|
E

E

V

G

A

P

P

A

G

Q

Q

L

H

E
|
E

I

V

N

D

L

F

R

K

H

F

G

D

D

D

P

A

I

L

E

K

L

T

A

A

D

D

Q

S

V

V

F

I

M

T

F

F

K

K

R

T

T

T

I

I

R

K

E

T

A

I

A

A

L

E

K

Q

H

H

G

G

I

L

Y

K

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

F

Q

F

G

G

Q

M

P

N

G

G

S

S

A

G

M

M

H

H

I

C

H

H

Q

Q

S

S

V

I

I

-

D

-

I

W

E

L

T

N

G

G

R

E

N

P

I

S

F

F

S

Y

S

D

P

E

D

N

G

A

-

P

-

Y

S

Q

P

L

S

S

K

E

E

T

F

C

F

M

A

N

F

Y

I

V

G

A

G

G

M

I

Q

L

H

K

Y

H

V

A

P

K

K

A

T

I

L

V

S

A

M

I

M

T

A

N

P

P

Y

T

V

V

N

N

S

S

Y

Y

R

K

R

R

L

L

T

V

P

P

D

G

M

Y

S

E

A

A

P

P

V

V

N

N

T

I

A

A

W

W

G

A

Y

N

D

S

N

N

R

R

T

S

T

A

A

I

F

I

R

R

I

V

P

P

V

A

S

A

D

R

P

G

V

K

A

G

R

T

R

R

I

I

E

E

N

F

R

R

L

A

P

P

S

D

S

P

D

S

A

C

N

N

P

P

Y

Y

L

L

A

A

L

F

A

T

A

V

S

M

L

L

G

A

C

A

G

G

Y

L

L

D

G

G

I

V

M

K

E

N

D

K

L

L

Q

D

P

A

T

P

P

E

P

P

S

V

E

E

D

R

T

N

-

I

-

F

-

A

-

M

-

S

-

E

A

A

N

E

E

K

G

K

E

E

I

L

-

G

-

I

-

E

D

S

L

V

P

P

R

A

G

N

L

L

L

K

E

A

A

A

I

L

S

D

L

E

L

L

E

E

S

N

E

N

P

D

S

V

L

L

A

K

E

N

V

A

F

L

S

G

A

K

E

H

F

I

I

F

A

E

I

S

Y

F

A

L

G

E

V

I

K

K

R

N

G

A

E

E

F

W

E

D

T

S

F

F

M

R

Q

T

V

S

I

V

S

T

P

D

W

W

E

E

binding 2-oxoglutaric acid: R86 (≠ E121), V92 (≠ T125), P169 (≠ D209), R172 (≠ I212), R173 (≠ D213), S189 (≠ L229)
binding magnesium ion: E137 (= E169), E192 (= E232), E199 (= E239)

Sites not aligning to the query:

binding 2-oxoglutaric acid: 16, 18, 32

8ufjB Glutamine synthetase (see paper)
32% identity, 77% coverage: 103:470/478 of query aligns to 66:436/444 of 8ufjB

query
sites
8ufjB

D

E

S

S

D

D

I

M

K

K

L

L

V

A

P

L

D

D

L

P

S

S

T

T

I

F

S

R

I

I

V

L

P

P

W

W

E

R

-

P

-

A

S

T

D

G

P

A

T

V

A

A

Q

R

V

I

I

L

C

G

D

D

I

V

V

Y

G

L

S

P

Q

D

G

G

E

N

Q

P

I

F

S

K

Y

G

T

D

P

P

R

R

N

Y

V

V

L

L

K

K

R

T

V

A

L

I

D

K

L

E

Y

A

R

E

Q

K

K

M

G

G

W

F

K

S

P

M

V

N

V

V

A

G

P

P

E
|
E

I

L

E
|
E

F

F

Y

F

L

L

-

F

-

K

V

L

A

D

Q

A

N

N

D

G

D

N

P

P

D

T

Y

T

P

E

L

L

R

T

P

D

P

Q

K

G

G

G

R

Y

S

-

G

-

R

-

S

-

I

-

L

-

G

-

Q

-

G

-

Y

F

S

D

I

F

A

A

G

P

I

L

N

D

E

L

F

G

D

Q

E

D

L

V

I

R

D

R

D

D

I

I

Y

D

H

Y

F

A

S

L

E

E

K

H

Q

M

G

G

L

F

E

Q

I

I

D

E

T

A

L

S

I

H

H

H

E
|
E

E

V

G

A

P

P

A

S

Q

Q

L

H

E
|
E

I

I

N

D

L

F

R

R

H

F

G

G

D

D

P

V

I

L

E

C

L

T

A

A

D

D

Q

N

V

V

F

V

M

T

F

F

K

K

R

Y

T

V

I

V

R

K

E

S

A

I

A

A

L

Y

K

H

H

K

G

G

I

Y

Y

Y

A

A

T

S

F

F

M

M

A

P

K

K

P

P

M

L

Q

F

G

G

Q

V

P

N

G

G

S

S

A

G

M

M

H
|
H

I

S

H

N

Q

Q

S

S

V

L

I

F

D

-

I

-

E

K

T

D

G

G

R

K

N

N

I

V

F

F

S

Y

S

D

P

P

D

D

G

-

S

T

P

P

S

T

K

K

-

L

-

S

-

Q

E

D

F

A

F

M

A

Y

F

Y

I

I

G

G

M

L

Q

L

H

K

Y

H

V

I

P

R

K

E

T

F

L

T

S

A

M

V

M

T

A

N

P

P

Y

V

V

V

N

N

S

S

Y

Y

R

K

R

R

L

L

T

V

P

P

D

G

M

Y

S

E

A

A

P

P

V

V

N

Y

T

I

A

S

W

W

G

S

Y

A

D

Q

N

N

R

R

T

S

A

L

F

I

R

R

I

I

P

P

V

A

S

T

D

R

P

G

V

N

A

G

R

T

R

R

I

I

E
|
E

N

L

R

R

L

C

P

P

S

D

S

P

D

A

A

C

N

N

P

P

Y

Y

L

L

A

A

L

F

A

A

A

L

S

M

L

L

G

R

C

A

G

G

Y

L

L

E

G

G

I

I

M

K

E

N

D

K

L

I

Q

D

P

P

T

G

P

E

P

P

S

T

-

N

-

V

-

N

-

I

-

F

-

H

-

L

-

S

-

D

E

K

D

E

T

R

A

E

N

E

E

R

G

G

E

I

I

R

D

S

L

L

P

P

R

A

G

D

L

L

L

K

E

E

A

A

I

I

S

D

L

E

L

M

E

K

S

G

E

S

P

K

S

F

L

V

A

K

E

E

V

A

F

L

S

G

A

E

E

H

F

V

I

F

A

S

I

H

Y

Y

A

L

G

C

V

A

K

K

R

E

G

M

E

E

F

W

E

D

T

E

F

Y

M

K

Q

A

V

V

I

V

S

H

P

P

W

W

E

E

binding magnesium ion: E132 (= E167), E134 (= E169), E189 (= E232), E196 (= E239), H245 (= H288), E333 (= E376)

7tdpA Structure of paenibacillus polymyxa gs bound to met-sox-p-adp (transition state complex) to 1.98 angstom (see paper)
33% identity, 78% coverage: 103:475/478 of query aligns to 63:436/439 of 7tdpA

query
sites
7tdpA

D

E

S

S

D

D

I

M

K

Y

L

L

V

Y

P

P

D

D

L

L

S

D

T

T

I

W

S

V

I

V

V

F

P

P

W

W

E

-

S

S

D

D

P

R

T

V

A

A

Q

R

V

L

I

I

C

C

D

D

I

I

V

Y

G

K

S

P

Q

D

G

G

E

S

Q

P

I

F

S

A

Y

G

T

D

P

P

R

R

N

G

V

I

L

L

K

K

R

R

V

V

L

L

D

K

L

E

Y

A

R

E

Q

E

K

L

G

G

W

Y

K

T

P

S

V

M

-
x
N

V

V

A
x
G

P

P

E
|
E

I

P

E
|
E

F

F

Y

F

L

L

V

F

A

K

Q

T

N

D

D

E

-

K

-

G

D

D

P

P

D

T

Y

T

P

E

L

L

R

N

P

D

P

Q

K

G

G

G

R

Y

S

-

G

-

R

-

S

-

I

-

L

-

G

-

Q

-

G

-

Y

F

S

D

I

L

A

A

G

P

I

M

N

D

E

L

F

G

D

E

E

N

L

C

I

R

D

R

D

E

I

I

Y

V

H

L

F

K

S

L

E

E

K

E

Q

M

G

G

L

F

E

E

I

I

D
x
E

T

A

L

S

I

H

H

H

E
|
E

E

V

G

A

P

P

A

G

Q

Q

L

H

E
|
E

I

I

N
x
D

L

F

R

K

H
x
Y

G

A

D

D

P

A

I

V

E

K

L

A

A

A

D

D

Q

Q

V

I

F

Q

M

T

F

F

K

K

R

L

T

V

I

V

R

K

E

T

A

I

A

A

L

R

K

Q

H

H

G

G

I

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

L

Q

F

G

G

Q

V

P

N

G
|
G

S

S

A

G

M

M

H
|
H

I

C

H
x
N

Q

Q

S
|
S

V

L

I

-

D

-

I

F

E

K

T

D

G

N

R

E

N

N

I

V

F

F

-

Y

-

D

S

E

S

T

P

D

D

E

G

L

S

G

P

L

S

S

K

Q

E

T

F

A

F

R

A

H

F

Y

I

M

G

A

G

G

M

I

Q

L

H

K

Y

H

V

A

P

R

K

A

T

M

L

A

S

A

M

I

M

T

A

N

P

P

Y

T

V

V

N

N

S

S

Y

Y

R

K

R
|
R

L

L

T

V

P

P

D

G

M

Y

S
x
E

A

A

P

P

V

C

N

Y

T

V

A

A

W

W

G

S

Y

A

D

S

N

N

R
|
R

T

S

T

P

A

M

F

I

R
|
R

I

I

P

P

V

A

S

S

D

R

P

G

V

L

A

S

R

T

R
|
R

I

V

E
|
E

N

V

R
|
R

L

N

P

P

S

D

S

P

D

A

A

A

N

N

P

P

Y

Y

L

L

A

A

L

L

A

A

A

V

S

M

L

L

G

R

C

A

G

G

Y

L

L

D

G

G

I

I

M

K

E

R

D

Q

L

M

Q

A

-

L

P

P

T

A

P

P

-

I

-

D

-

R

-

N

-

I

-

Y

-

V

-

M

P

S

S

E

E

E

D

E

T

R

A

I

N

E

E

E

G

G

E

I

I

P

D

S

L

L

P

P

R

A

G

D

L

L

L

K

E

E

A

A

I

L

S

S

L

E

L

L

E

I

S

R

E

S

P

E

S

V

L

I

A

S

E

D

V

A

F

L

S

G

A

D

E

H

F

A

I

L

A

A

I

Y

Y

F

A

Y

G

E

V

L

K

K

R

E

G

I

E

E

F

W

E

D

T

M

F

Y

M

R

Q

T

V

Q

I

V

S

H

P

Q

W

W

E

E

R

R

E

D

F

Q

L

Y

L

L

binding adenosine-5'-diphosphate: N123 (vs. gap), G125 (≠ A165), E127 (= E167), E179 (≠ D227), D193 (≠ N241), Y196 (≠ H244), N242 (≠ H290), S244 (= S292), R316 (= R364), R326 (= R374)
binding magnesium ion: E127 (= E167), E127 (= E167), E129 (= E169), E184 (= E232), E191 (= E239), E191 (= E239), H240 (= H288), E328 (= E376)
binding l-methionine-s-sulfoximine phosphate: E127 (= E167), E129 (= E169), E184 (= E232), E191 (= E239), G236 (= G284), H240 (= H288), R293 (= R341), E299 (≠ S347), R311 (= R359), R330 (= R378)

7tfaB Glutamine synthetase (see paper)
32% identity, 78% coverage: 103:475/478 of query aligns to 63:438/441 of 7tfaB

query
sites
7tfaB

D

E

S

S

D

D

I

M

K

Y

L

L

V

Y

P

P

D

D

L

L

S

D

T

T

I

W

S

V

I

V

V

F

P

P

W

W

-

V

E

T

S

S

D

D

P

R

T

V

A

A

Q

R

V

L

I

I

C

C

D

D

I

I

V

Y

G

K

S

P

Q

D

G

G

E

S

Q

P

I

F

S

A

Y

G

T

D

P

P

R

R

N

G

V

I

L

L

K

K

R

R

V

V

L

L

D

K

L

E

Y

A

R

E

Q

E

K

L

G

G

W

Y

K

T

P

S

V

M

-

N

V

V

A

G

P

P

E
|
E

I

P

E
|
E

F

F

Y

F

L

L

V

F

A

K

Q

T

N

D

D

E

-

K

-

G

D

D

P

P

D

T

Y

T

P

E

L

L

R

N

P

D

P

Q

K

G

G

G

R
x
Y

S

-

G

-

R

-

S

-

I

-

L

-

G

-

Q

-

G

-

Y

F

S

D

I

L

A

A

G

P

I

M

N

D

E

L

F

G

D

E

E

N

L

C

I

R

D

R

D

E

I

I

Y

V

H

L

F

K

S

L

E

E

K

E

Q

M

G

G

L

F

E

E

I

I

D

E

T

A

L

S

I

H

H

H

E
|
E

E
x
V

G

A

P

P

A

G

Q

Q

L

H

E
|
E

I

I

N

D

L

F

R

K

H

Y

G

A

D

D

P

A

I

V

E

K

L

A

A

A

D

D

Q

Q

V

I

F

Q

M

T

F

F

K

K

R

L

T

V

I

V

R

K

E

T

A

I

A

A

L

R

K

Q

H

H

G

G

I

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

L

Q

F

G

G

Q

V

P
x
N

G
|
G

S

S

A

G

M

M

H
|
H

I

C

H

N

Q

Q

S

S

V

L

I

-

D

-

I

F

E

K

T

D

G

N

R

E

N

N

I

V

F

F

-

Y

-

D

S

E

S

T

P

D

D

E

G

L

S

G

P

L

S

S

K

Q

E

T

F

A

F

R

A

H

F

Y

I

M

G

A

G

G

M

I

Q

L

H

K

Y

H

V

A

P

R

K

A

T

M

L

A

S

A

M

I

M

T

A

N

P

P

Y

T

V

V

N

N

S

S

Y

Y

R

K

R
|
R

L

L

T

V

P

P

D
x
G

M
x
Y

S
x
E

A

A

P

P

V

C

N

Y

T

V

A

A

W

W

G

S

Y

A

D

S

N

N

R
|
R

T

S

T

P

A

M

F

I

R

R

I

I

P

P

V

A

S

S

D

R

P

G

V

L

A

S

R

T

R

R

I

V

E
|
E

N

V

R

R

L

N

P

P

S

D

S

P

D

A

A

A

N

N

P

P

Y

Y

L

L

A

A

L

L

A

A

A

V

S

M

L

L

G

R

C

A

G

G

Y

L

L

D

G

G

I

I

M

K

E

R

D

Q

L

M

Q

A

-

L

P

P

T

A

P

P

-

I

-

D

-

R

-

N

-

I

-

Y

-

V

-

M

P

S

S

E

E

E

D

E

T

R

A

I

N

E

E

E

G

G

E

I

I

P

D

S

L

L

P

P

R

A

G

D

L

L

L

K

E

E

A

A

I

L

S

S

L

E

L

L

E

I

S

R

E

S

P

E

S

V

L

I

A

S

E

D

V

A

F

L

S

G

A

D

E

H

F

A

I

L

A

A

I

Y

Y

F

A

Y

G

E

V

L

K

K

R

E

G

I

E

E

F

W

E

D

T
x
M

F

Y

M

R

Q

T

V

Q

I

V

S

H

P

Q

W

W

E

E

R

R

E

D

F

Q

L

Y

L

L

binding glutamine: E131 (= E169), Y153 (≠ R189), E186 (= E232), G238 (= G284), H242 (= H288), R295 (= R341), E301 (≠ S347)
binding magnesium ion: E129 (= E167), E131 (= E169), E186 (= E232), E193 (= E239), H242 (= H288), E330 (= E376)
binding : V187 (≠ E233), N237 (≠ P283), G299 (≠ D345), Y300 (≠ M346), R313 (= R359), M424 (≠ T461)

Sites not aligning to the query:

binding : 58, 60

4lnkA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of gs-glutamate-amppcp complex (see paper)
32% identity, 77% coverage: 103:472/478 of query aligns to 64:437/443 of 4lnkA

query
sites
4lnkA

D

E

S

S

D

D

I

M

K

Y

L

L

V

Y

P

P

D

D

L

L

S

N

T

T

I

F

S

V

I

I

V

F

P

P

W

W

-

T

-

A

E

E

S

K

D

G

P

K

T

V

A

A

Q

R

V

F

I

I

C

C

D

D

I

I

V

Y

G

N

S

P

Q

D

G

G

E

T

Q

P

I

F

S

E

Y

G

T

D

P

P

R

R

N

N

V

N

L

L

K

K

R

R

V

I

L

L

D

K

L

E

Y

M

R

E

Q

D

K

L

G

G

W

F

K

S

P

D

V

F

-

N

V

L

A

G

P

P

E
|
E

I

P

E
|
E

F

F

Y

F

L

L

V

F

A

K

Q

L

N

D

D

E

D

-

P

-

D

-

Y

-

P

-

L

-

R

-

P

-

K

K

G

G

R

E

S

P

G

T

R

L

S

E

I

L

L

N

G

D

G

K

Q

G

G

G

Y

Y

-

F

S

D

I

L

A

A

G

P

I

T

N

D

E

L

F

G

D

E

E

N

L

C

I

R

D

R

D

D

I

I

Y

V

H

L

F

E

S

L

E

E

K

E

Q

M

G

G

L

F

E

E

I

I

D

E

T

A

L

S

I

H

H

H

E
|
E

E
x
V

G

A

P

P

A

G

Q

Q

L

H

E
|
E

I

I

N

D

L
x
F

R

K

H
x
Y

G

A

D

G

P

A

I

V

E

R

L

S

A

C

D

D

Q

D

V

I

F

Q

M

T

F

F

K

K

R

L

T

V

I

V

R

K

E

T

A

I

A

A

L

R

K

K

H

H

G

G

I

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

L

Q

F

G

G

Q

V

P
x
N

G
|
G

S

S

A
x
G

M

M

H
|
H

I

C

H
x
N

Q

L

S
|
S

V

L

I

F

D

-

I

-

E

K

T

N

G

G

R

V

N

N

I

A

F

F

-

F

-

D

S

E

S

N

P

A

D

D

G

L

S

Q

P

L

S

S

K

E

E

T

F

A

F

K

A

H

F

F

I

I

G

A

G

G

M

I

Q

V

H

K

Y

H

V

A

P

T

K

S

T

F

L

T

S

A

M

V

M

T

A

N

P

P

Y

T

V

V

N

N

S

S

Y

Y

R

K

R

R

L

L

T

V

P

P

D

G

M

Y

S
x
E

A

A

P

P

V

C

N

Y

T

V

A

A

W

W

G

S

Y

A

D

Q

N

N

R
|
R

T

S

T

P

A

L

F

I

R

R

I

I

P

P

V

A

S
|
S

D

R

P

G

V

I

A
x
S

R

T

R
|
R

I

V

E
|
E

N

V

R
|
R

L

S

P

V

S

D

S

P

D

A

A

A

N

N

P

P

Y

Y

L

L

A

A

L

L

A

S

A

V

S

L

L

L

G

A

C

A

G

G

Y

L

L

D

G

G

I

I

M

K

E

N

D

K

L

L

Q

E

P

A

T

P

P

A

P

P

-

I

-

D

-

R

-

N

-

I

-

Y

-

V

-

M

-

S

S

K

E

E

D

E

T

R

A

M

N

E

E

N

G

G

E

I

I

V

D

D

L

L

P

P

R

A

G

T

L

L

L

A

E

E

A

A

I

L

S

E

L

E

L

F

E

K

S

S

E

N

P

E

S

V

L

M

A

V

E

K

V

A

F

L

S

G

A

E

E

H

F

L

I

F

A

E

I

H

Y

F

A

I

G

E

V

A

K

K

R

E

G

I

E

E

F

W

E

D

T

M

F

F

M

R

Q

T

V

Q

I

V

S

H

P

P

W

W

E

E

R

R

E

E

active site: E131 (= E167), E133 (= E169), E188 (= E232), E195 (= E239), H244 (= H288), R315 (= R359), E332 (= E376), R334 (= R378)
binding adenosine-5'-diphosphate: F198 (≠ L242), Y200 (≠ H244), N246 (≠ H290), S248 (= S292), S324 (= S368), S328 (≠ A372), R330 (= R374)
binding glutamic acid: E133 (= E169), E188 (= E232), V189 (≠ E233), N239 (≠ P283), G240 (= G284), G242 (≠ A286), E303 (≠ S347)
binding magnesium ion: E131 (= E167), E188 (= E232), E195 (= E239), H244 (= H288), E332 (= E376)

Sites not aligning to the query:

active site: 52
binding adenosine-5'-diphosphate: 43, 50

4lniA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of the transition state complex (see paper)
32% identity, 77% coverage: 103:472/478 of query aligns to 64:437/443 of 4lniA

query
sites
4lniA

D

E

S

S

D

D

I

M

K

Y

L

L

V

Y

P

P

D

D

L

L

S

N

T

T

I

F

S

V

I

I

V

F

P

P

W

W

-

T

-

A

E

E

S

K

D

G

P

K

T

V

A

A

Q

R

V

F

I

I

C

C

D

D

I

I

V

Y

G

N

S

P

Q

D

G

G

E

T

Q

P

I

F

S

E

Y

G

T

D

P

P

R

R

N

N

V

N

L

L

K

K

R

R

V

I

L

L

D

K

L

E

Y

M

R

E

Q

D

K

L

G

G

W

F

K

S

P

D

V

F

-

N

V

L

A

G

P

P

E
|
E

I

P

E
|
E

F

F

Y

F

L

L

V

F

A

K

Q

L

N

D

D

E

D

-

P

-

D

-

Y

-

P

-

L

-

R

-

P

-

K

K

G

G

R

E

S

P

G

T

R

L

S

E

I

L

L

N

G

D

G

K

Q

G

G

G

Y

Y

-

F

S

D

I

L

A

A

G

P

I

T

N

D

E

L

F

G

D

E

E

N

L

C

I

R

D

R

D

D

I

I

Y

V

H

L

F

E

S

L

E

E

K

E

Q

M

G

G

L

F

E

E

I

I

D
x
E

T

A

L

S

I

H

H

H

E
|
E

E

V

G

A

P

P

A

G

Q

Q

L

H

E
|
E

I

I

N
x
D

L

F

R

K

H
x
Y

G

A

D

G

P

A

I

V

E

R

L

S

A

C

D

D

Q

D

V

I

F

Q

M

T

F

F

K

K

R

L

T

V

I

V

R

K

E

T

A

I

A

A

L

R

K

K

H

H

G

G

I

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

L

Q

F

G

G

Q

V

P

N

G

G

S

S

A

G

M

M

H
|
H

I

C

H
x
N

Q

L

S
|
S

V

L

I

F

D

-

I

-

E

K

T

N

G

G

R

V

N

N

I

A

F

F

-

F

-

D

S

E

S

N

P

A

D

D

G

L

S

Q

P

L

S

S

K

E

E

T

F

A

F

K

A

H

F

F

I

I

G

A

G

G

M

I

Q

V

H

K

Y

H

V

A

P

T

K

S

T

F

L

T

S

A

M

V

M

T

A

N

P

P

Y

T

V

V

N

N

S

S

Y

Y

R

K

R
|
R

L

L

T

V

P

P

D

G

M

Y

S
x
E

A

A

P

P

V

C

N

Y

T

V

A

A

W

W

G

S

Y

A

D

Q

N

N

R
|
R

T

S

T

P

A

L

F

I

R
|
R

I

I

P

P

V

A

S

S

D

R

P

G

V

I

A

S

R

T

R
|
R

I

V

E
|
E

N

V

R
|
R

L

S

P

V

S

D

S

P

D

A

A

A

N

N

P

P

Y

Y

L

L

A

A

L

L

A

S

A

V

S

L

L

L

G

A

C

A

G

G

Y

L

L

D

G

G

I

I

M

K

E

N

D

K

L

L

Q

E

P

A

T

P

P

A

P

P

-

I

-

D

-

R

-

N

-

I

-

Y

-

V

-

M

-

S

S

K

E

E

D

E

T

R

A

M

N

E

E

N

G

G

E

I

I

V

D

D

L

L

P

P

R

A

G

T

L

L

L

A

E

E

A

A

I

L

S

E

L

E

L

F

E

K

S

S

E

N

P

E

S

V

L

M

A

V

E

K

V

A

F

L

S

G

A

E

E

H

F

L

I

F

A

E

I

H

Y

F

A

I

G

E

V

A

K

K

R

E

G

I

E

E

F

W

E

D

T

M

F

F

M

R

Q

T

V

Q

I

V

S

H

P

P

W

W

E

E

R

R

E

E

active site: E131 (= E167), E133 (= E169), E188 (= E232), E195 (= E239), H244 (= H288), R315 (= R359), E332 (= E376), R334 (= R378)
binding adenosine-5'-diphosphate: E131 (= E167), E183 (≠ D227), D197 (≠ N241), Y200 (≠ H244), N246 (≠ H290), S248 (= S292), R320 (= R364), R330 (= R374)
binding magnesium ion: E131 (= E167), E131 (= E167), E133 (= E169), E188 (= E232), E195 (= E239), E195 (= E239), H244 (= H288), E332 (= E376)
binding l-methionine-s-sulfoximine phosphate: E133 (= E169), E188 (= E232), H244 (= H288), R297 (= R341), E303 (≠ S347), R315 (= R359), R334 (= R378)

Sites not aligning to the query:

active site: 52

P12425 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Bacillus subtilis (strain 168) (see 5 papers)
32% identity, 77% coverage: 103:472/478 of query aligns to 65:438/444 of P12425

query
sites
P12425

D

E

S

S

D

D

I

M

K

Y

L

L

V

Y

P

P

D

D

L

L

S

N

T

T

I

F

S

V

I

I

V

F

P

P

W

W

-

T

-

A

E

E

S

K

D

G

P

K

T

V

A

A

Q

R

V

F

I

I

C

C

D

D

I

I

V

Y

G

N

S

P

Q

D

G

G

E

T

Q

P

I

F

S

E

Y

G

T

D

P

P

R

R

N

N

V

N

L

L

K

K

R

R

V

I

L

L

D

K

L

E

Y

M

R

E

Q

D

K

L

G

G

W

F

K

S

P

D

V

F

-

N

V

L

A

G

P

P

E
|
E

I

P

E
|
E

F

F

Y

F

L

L

V

F

A

K

Q

L

N

D

D

E

D

-

P

-

D

-

Y

-

P

-

L

-

R

-

P

-

K

K

G

G

R

E

S

P

G

T

R

L

S

E

I

L

L

N

G

D

G

K

Q

G

G

G

Y

Y

-

F

S

D

I

L

A

A

G

P

I

T

N

D

E

L

F

G

D

E

E

N

L

C

I

R

D

R

D

D

I

I

Y

V

H

L

F

E

S

L

E

E

K

E

Q

M

G

G

L

F

E

E

I

I

D

E

T

A

L

S

I

H

H

H

E
|
E

E
x
V

G

A

P

P

A

G

Q

Q

L

H

E
|
E

I

I

N

D

L

F

R

K

H

Y

G

A

D

G

P

A

I

V

E

R

L

S

A

C

D

D

Q

D

V

I

F

Q

M

T

F

F

K

K

R

L

T

V

I

V

R

K

E

T

A

I

A

A

L

R

K

K

H

H

G

G

I

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

L

Q

F

G

G

Q

V

P

N

G
|
G

S

S

A

G

M

M

H
|
H

I

C

H

N

Q

L

S

S

V

L

I

F

D

-

I

-

E

K

T

N

G

G

R

V

N

N

I

A

F

F

-

F

-

D

S

E

S

N

P

A

D

D

G

L

S

Q

P

L

S

S

K

E

E

T

F

A

F

K

A

H

F

F

I

I

G

A

G

G

M

I

Q

V

H

K

Y

H

V

A

P

T

K

S

T

F

L

T

S

A

M

V

M

T

A

N

P

P

Y

T

V

V

N

N

S

S

Y

Y

R

K

R

R

L

L

T

V

P

P

D
x
G

M

Y

S
x
E

A

A

P
|
P

V

C

N

Y

T

V

A

A

W

W

G

S

Y

A

D

Q

N

N

R

R

T

S

T

P

A

L

F

I

R

R

I

I

P

P

V

A

S

S

D

R

P

G

V

I

A

S

R

T

R

R

I

V

E
|
E

N

V

R

R

L

S

P

V

S

D

S

P

D

A

A

A

N

N

P

P

Y

Y

L

L

A

A

L

L

A

S

A

V

S

L

L

L

G

A

C

A

G

G

Y

L

L

D

G

G

I

I

M

K

E

N

D

K

L

L

Q

E

P

A

T

P

P

A

P

P

-

I

-

D

-

R

-

N

-

I

-

Y

-

V

-

M

-

S

S

K

E

E

D

E

T

R

A

M

N

E

E

N

G

G

E

I

I

V

D

D

L

L

P

P

R

A

G

T

L

L

L

A

E

E

A

A

I

L

S

E

L

E

L

F

E

K

S

S

E

N

P

E

S

V

L

M

A

V

E

K

V

A

F

L

S

G

A

E

E

H

F

L

I

F

A

E

I

H

Y

F

A

I

G

E

V

A

K

K

R

E

G

I

E
|
E

F

W

E

D

T

M

F

F

M

R

Q

T

V

Q

I

V

S

H

P

P

W

W

E

E

R

R

E

E

E132 (= E167) binding Mg(2+)
E134 (= E169) binding Mg(2+)
E189 (= E232) binding Mg(2+)
V190 (≠ E233) mutation to A: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant partially relieves expression of the glnRA-lacZ fusion, but has no effect on the TnrA-dependent regulation of amtB-lacZ fusion. Resistant to inhibition by MetSox.
E196 (= E239) binding Mg(2+)
G241 (= G284) binding L-glutamate
H245 (= H288) binding Mg(2+)
G302 (≠ D345) mutation to E: Unable to form stable complex with TnrA. In the presence of glutamine, amtB-lacZ fusion is only 4-fold regulated by TnrA, whereas glnRA-lacZ fusion is derepressed. This mutant retains enzymatic specific activity with a 2-fold decrease of the affinity for glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.
E304 (≠ S347) mutation to A: Highly resistant to Met-Sox inhibition. 8- and 2-fold increase of the affinity for glutamate and ATP, respectively. Strong decrease of the affinity for ammonium.
P306 (= P349) mutation to H: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant completely derepresses glnRA-lacZ fusion, whereas amtB-lacZ fusion expression is only partially derepresses.
E333 (= E376) binding Mg(2+)
E424 (= E458) mutation to K: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion. Although it is defective in regulation, this mutant retains enzymatic specific activity and similar affinity for ATP, glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
59 G→R: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion.
62 Important for inhibition by glutamine; R→A: Highly resistant to inhibition by glutamine and AMP. Regulation by TnrA and GlnR is abolished. Only small differences (less than 2-fold) in its steady-state kinetic constants compared with the wild-type. Similar sensitivity to Met-Sox that compared to the wild-ytpe.

4s0rD Structure of gs-tnra complex (see paper)
32% identity, 77% coverage: 103:472/478 of query aligns to 68:441/447 of 4s0rD

query
sites
4s0rD

D

E

S

S

D

D

I

M

K

Y

L

L

V

Y

P

P

D

D

L

L

S

N

T

T

I

F

S

V

I

I

V

F

P

P

W

W

-

T

-

A

E

E

S

K

D

G

P

K

T

V

A

A

Q

R

V

F

I

I

C

C

D

D

I

I

V

Y

G

N

S

P

Q

D

G

G

E

T

Q

P

I

F

S

E

Y

G

T

D

P

P

R

R

N

N

V

N

L

L

K

K

R

R

V

I

L

L

D

K

L

E

Y

M

R

E

Q

D

K

L

G

G

W

F

K

S

P

D

V

F

-

N

V

L

A

G

P

P

E
|
E

I

P

E
|
E

F

F

Y

F

L

L

V

F

A

K

Q

L

N

D

D

E

D

-

P

-

D

-

Y

-

P

-

L

-

R

-

P

-

K

K

G

G

R

E

S

P

G

T

R

L

S

E

I

L

L

N

G

D

G

K

Q

G

G

G

Y

Y

-

F

S
x
D

I

L

A

A

G

P

I

T

N

D

E

L

F

G

D

E

E

N

L

C

I

R

D

R

D

D

I

I

Y

V

H

L

F

E

S

L

E

E

K

E

Q

M

G

G

L

F

E

E

I

I

D

E

T

A

L

S

I

H

H

H

E
|
E

E

V

G

A

P

P

A

G

Q

Q

L

H

E
|
E

I

I

N

D

L

F

R

K

H

Y

G

A

D

G

P

A

I

V

E

R

L

S

A

C

D

D

Q

D

V

I

F

Q

M

T

F

F

K

K

R

L

T

V

I

V

R

K

E

T

A

I

A

A

L

R

K

K

H

H

G

G

I

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

L

Q

F

G
|
G

Q
x
V

P
x
N

G

G

S

S

A

G

M

M

H
|
H

I

C

H

N

Q

L

S

S

V

L

I

F

D

-

I

-

E

K

T

N

G

G

R

V

N

N

I

A

F

F

-

F

-

D

S

E

S

N

P

A

D

D

G

L

S

Q

P

L

S

S

K

E

E

T

F

A

F

K

A

H

F

F

I

I

G

A

G

G

M

I

Q

V

H

K

Y

H

V

A

P

T

K

S

T

F

L

T

S

A

M

V

M

T

A

N

P

P

Y

T

V

V

N

N

S

S

Y

Y

R

K

R
|
R

L

L

T

V

P

P

D
x
G

M
x
Y

S
x
E

A

A

P

P

V

C

N

Y

T

V

A

A

W

W

G

S

Y

A

D

Q

N

N

R
|
R

T

S

T

P

A

L

F

I

R

R

I

I

P

P

V

A

S

S

D

R

P

G

V

I

A

S

R

T

R

R

I

V

E
|
E

N

V

R
|
R

L

S

P

V

S

D

S

P

D

A

A

A

N

N

P

P

Y

Y

L

L

A

A

L

L

A

S

A

V

S

L

L

L

G

A

C

A

G

G

Y

L

L

D

G

G

I

I

M

K

E

N

D

K

L

L

Q

E

P

A

T

P

P

A

P

P

-

I

-

D

-

R

-

N

-

I

-
x
Y

-

V

-

M

-

S

S

K

E

E

D

E

T

R

A

M

N

E

E

N

G

G

E

I

I

V

D

D

L

L

P

P

R

A

G

T

L

L

L

A

E

E

A

A

I

L

S

E

L

E

L

F

E

K

S

S

E

N

P

E

S

V

L

M

A

V

E

K

V

A

F

L

S

G

A

E

E

H

F

L

I

F

A

E

I
x
H

Y

F

A

I

G

E

V

A

K

K

R

E

G
x
I

E

E

F

W

E

D

T
x
M

F

F

M

R

Q

T

V

Q

I

V

S

H

P

P

W

W

E

E

R

R

E

E

active site: E135 (= E167), E137 (= E169), E192 (= E232), E199 (= E239), H248 (= H288), R319 (= R359), E336 (= E376), R338 (= R378)
binding glutamine: E137 (= E169), E192 (= E232), R301 (= R341), E307 (≠ S347)
binding magnesium ion: E135 (= E167), E135 (= E167), E199 (= E239), H248 (= H288), H248 (= H288), E336 (= E376), H419 (≠ I450)
binding : D161 (≠ S201), G241 (= G281), V242 (≠ Q282), N243 (≠ P283), G305 (≠ D345), Y306 (≠ M346), Y376 (vs. gap), I426 (≠ G457), M430 (≠ T461)

Sites not aligning to the query:

active site: 56
binding magnesium ion: 66
binding : 63, 64, 65, 66

7tf9S L. Monocytogenes gs(14)-q-glnr peptide (see paper)
32% identity, 77% coverage: 103:472/478 of query aligns to 64:437/443 of 7tf9S

query
sites
7tf9S

D

E

S

S

D

D

I

M

K

Y

L

L

V

F

P

P

D

D

L

L

S

D

T

T

I

W

S

V

I

V

V

F

P

P

W

W

-

T

-

A

E

E

S

K

D

G

P

K

T

V

A

A

Q

R

V

M

I

I

C

C

D

D

I

I

V

Y

G

N

S

P

Q

D

G

M

E

T

Q

P

I

F

S

A

Y

G

T

D

P

P

R

R

N

A

V

N

L

L

K

K

R

R

V

V

L

L

D

K

L

E

Y

M

R

E

Q

E

K

L

G

G

W

F

K

T

P

E

V

F

-

N

V

L

A

G

P

P

E
|
E

I

P

E
|
E

F

F

Y

F

L

L

V

F

A

K

Q

L

N

D

D

E

D

N

P

-

D

-

Y

-

P

-

L

-

R

R

P

R

P

P

K

T

G

L

R

E

S

L

G

N

R

D

S

S

I

-

L

-

G

-

G

G

Q

G

G
x
Y

Y

F

S

D

I

L

A

A

G

P

I

T

N

D

E

L

F

G

D

E

E

N

L

C

I

R

D

R

D

D

I

I

Y

V

H

L

F

E

S

L

E

E

K

E

Q

M

G

G

L

F

E

E

I

I

D

E

T

A

L

S

I

H

H

H

E
|
E

E

V

G

A

P

P

A

G

Q

Q

L

H

E
|
E

I

I

N

D

L

F

R

K

H

Y

G

E

D

D

P

A

I

I

E

T

L

A

A

C

D

D

Q

S

V

I

F

Q

M

T

F

F

K

K

R

L

T

V

I

V

R

K

E

T

A

I

A

A

L

R

K

K

H

H

G

G

I

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

L

Q

F

G

G

Q

V

P

N

G
|
G

S

S

A
x
G

M

M

H
|
H

I

F

H

N

Q

M

S

S

V

L

I

F

D

N

I

-

E

E

T

K

G

G

R

N

N

A

I

F

F

F

-

D

S

E

S

S

P

G

D

E

G

L

S

E

P

L

S

S

K

Q

E

T

F

A

F

Y

A

H

F

F

I

L

G

A

G

G

M

M

Q

L

H

K

Y

H

V

A

P

R

K

G

T

Y

L

T

S

A

M

V

M

T

A

N

P

P

Y

T

V

I

N

N

S

S

Y

F

R

K

R
|
R

L

L

T

V

P

P

D

G

M

Y

S
x
E

A

A

P

P

V

C

N

Y

T

I

A

A

W

W

G

S

Y

G

D

K

N

N

R

R

T

S

T

P

A

L

F

V

R

R

I

V

P

P

V

S

S

S

D

R

P

G

V

L

A

S

R

T

R

R

I

L

E
|
E

N

L

R

R

L

S

P

V

S

D

S

P

D

S

A

A

N

N

P

P

Y

Y

L

L

A

A

L

M

A

A

A

V

S

L

L

L

G

K

C

A

G

G

Y

L

L

S

G

G

I

I

M

K

E

D

D

E

L

L

Q

T

P

P

T

P

P

A

P

P

-

V

-

D

-

R

-

N

-

I

-

Y

-

G

-

M

-

N

S

E

E

E

D

E

T

R

A

E

N

A

E

T

G

G

E

I

I

Y

D

D

L

L

P

P

R

E

G

S

L

L

L

G

E

H

A

A

I

L

S

I

L

E

L

L

E

E

S

K

E

N

P

E

S

I

L

I

A

K

E

D

V

G

F

L

S

G

A

E

E

H

F

I

I

F

A

E

I

H

Y

F

A

I

G
x
E

V

A

K

K

R

T

G
x
I

E

E

F

C

E

D

T
x
M

F

F

M

R

Q

T

V

A

I

V

S

H

P

P

W

W

E

E

R

R

E

E

binding glutamine: E133 (= E169), Y155 (≠ G199), E188 (= E232), G240 (= G284), G242 (≠ A286), R297 (= R341), E303 (≠ S347)
binding magnesium ion: E131 (= E167), E133 (= E169), E188 (= E232), E195 (= E239), H244 (= H288), E332 (= E376)
binding : E418 (≠ G453), I422 (≠ G457), M426 (≠ T461)

Sites not aligning to the query:

binding : 59, 60

7tenA Glutamine synthetase (see paper)
32% identity, 77% coverage: 103:472/478 of query aligns to 63:436/442 of 7tenA

query
sites
7tenA

D

E

S

S

D

D

I

M

K

Y

L

L

V

F

P

P

D

D

L

L

S

D

T

T

I

W

S

V

I

V

V

F

P

P

W

W

-

T

-

A

E

E

S

K

D

G

P

K

T

V

A

A

Q

R

V

M

I

I

C

C

D

D

I

I

V

Y

G

N

S

P

Q

D

G

M

E

T

Q

P

I

F

S

A

Y

G

T

D

P

P

R

R

N

A

V

N

L

L

K

K

R

R

V

V

L

L

D

K

L

E

Y

M

R

E

Q

E

K

L

G

G

W

F

K

T

P

E

V

F

-

N

V

L

A
x
G

P

P

E
|
E

I

P

E
|
E

F

F

Y

F

L

L

V

F

A

K

Q

L

N

D

D

E

D

N

P

-

D

-

Y

-

P

-

L

-

R

R

P

R

P

P

K

T

G

L

R

E

S

L

G

N

R

D

S

S

I

-

L

-

G

-

G

G

Q

G

G

Y

Y

F

S

D

I

L

A

A

G

P

I

T

N

D

E

L

F

G

D

E

E

N

L

C

I

R

D

R

D

D

I

I

Y

V

H

L

F

E

S

L

E

E

K

E

Q

M

G

G

L

F

E

E

I

I

D
x
E

T

A

L

S

I

H

H

H

E
|
E

E

V

G

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binding adenosine-5'-diphosphate: G128 (≠ A165), E130 (= E167), E182 (≠ D227), D196 (≠ N241), F197 (≠ L242), K198 (≠ R243), Y199 (≠ H244), N245 (≠ H290), S247 (= S292), R319 (= R364), S327 (≠ A372), R329 (= R374)
binding l-methionine-s-sulfoximine phosphate: E130 (= E167), E132 (= E169), E187 (= E232), E194 (= E239), N238 (≠ P283), G239 (= G284), H243 (= H288), R296 (= R341), E302 (≠ S347), R314 (= R359), R333 (= R378)

A0R083 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
32% identity, 77% coverage: 103:470/478 of query aligns to 64:439/446 of A0R083

query
sites
A0R083

D

E

S

S

D

D

I

T

K

V

L

A

V

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P

P

D

D

L

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N

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W

Y

E

E

K363 (≠ E403) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

8ooxB Glutamine synthetase (see paper)
29% identity, 87% coverage: 54:470/478 of query aligns to 24:430/438 of 8ooxB

query
sites
8ooxB

D

D

L

V

A

Q

G

G

V

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P

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G

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K

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M

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|
E

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L

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|
E

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|
E

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I

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K

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H

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I

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N

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I

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H

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Q

W

W

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binding magnesium ion: E129 (= E169), E183 (= E232), E190 (= E239)

8oozA Glutamine synthetase (see paper)
31% identity, 77% coverage: 104:470/478 of query aligns to 57:422/430 of 8oozA

query
sites
8oozA

S

S

D

D

I

M

K

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D

D

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G

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|
E

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M

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|
H

I

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x
N

Q

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|
S

V

L

I

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D

-

I

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K

T

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G

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K

N

N

I

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F

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S

S

K

D

E

T

F

L

F

R

A

Y

F

F

I

V

G

G

M

V

Q

L

H

K

Y

H

V

I

P

R

K

A

T

I

L

T

S

A

M

I

M

T

A

N

P

P

Y

L

V

V

N

N

S

S

Y

Y

R

K

R

R

L

L

T

V

P

P

D

G

M

Y

S

E

A

A

P

P

V

V

N

Y

T

I

A

T

W

W

G

S

Y

G

D

P

N

N

R

R

T

S

A

L

F

I

R

R

I

V

P

P

V

A

S

P

D

R

P

G

V

N

A
x
S

R

T

R
|
R

I

I

E
|
E

N

I

R

R

L

S

P

P

S

D

S

P

D

S

A

C

N

N

P

P

Y

Y

L

L

A

A

L

F

A

A

S

I

L

L

G

A

C

A

G

G

Y

L

L

D

G

G

I

V

M

K

E

N

D

K

L

I

Q

E

P

P

-

P

-

E

-

R

-

V

-

E

-

K

-

N

-

I

-

Y

T

K

P

L

P

T

S

E

E

E

D

E

T

R

A

E

N

K

E

L

G

G

E

I

I

G

D

M

L

L

P

P

R

G

G

T

L

L

L

K

E

E

A

A

I

I

S

E

L

C

L

F

E

K

S

E

E

D

P

E

S

L

L

L

A

V

E

S

V

A

F

L

S

G

A

E

E

H

F

V

I

S

A

Q

I

S

Y

I

A

I

G

N

V

V

K

A

R

M

G

A

E

D

F

W

E

D

T

S

F

Y

M

R

Q

T

V

Q

I

V

S

H

P

Q

W

W

E

E

binding adenosine-5'-triphosphate: G117 (≠ A165), E170 (≠ D227), F185 (≠ L242), K186 (≠ R243), Y187 (≠ H244), N233 (≠ H290), S235 (= S292), S315 (≠ A372), R317 (= R374)
binding magnesium ion: E119 (= E167), H231 (= H288), E319 (= E376)

P9WN37 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
31% identity, 78% coverage: 103:476/478 of query aligns to 64:446/446 of P9WN37

query
sites
P9WN37

D

E

S

S

D

D

I

T

K

V

L

A

V

H

P

P

D

D

L

P

S

S

T

T

I

F

S

Q

I

V

V

L

P

P

W

W

E

A

S

T

D

S

P

S

-

G

-

H

T

S

A

A

Q

R

V

M

I

F

C

C

D

D

I

I

V

T

G

M

S

P

Q

D

G

G

E

S

Q

P

I

S

S

W

Y

A

T

D

P

P

R

R

N

H

V

V

L

L

K

R

R

R

V

Q

L

L

D

T

L

K

Y

A

R

G

Q

E

K

L

G

G

W

F

K

S

P

C

V

Y

V

V

A

H

P

P

E

E

I

I

E

E

F

F

Y

F

L

L

V

L

A

K

Q

P

N

G

-

P

D

E

D

D

P

G

D

S

Y

V

P

P

L

V

R

-

P

-

P

P

K

V

G

D

R

N

S

A

G

G

R

-

S

-

I

-

L

Y

G

F

G

D

Q

Q

G

A

Y

V

S

H

I

D

A

S

G

A

I

L

N

N

E

F

F

R

D

R

E

H

L

A

I

I

D

D

D

A

I

L

Y

-

H

-

F

-

S

-

E

E

K

F

Q

M

G

G

L

I

E

S

I

V

D

E

T

F

L

S

I

H

H

H

E

E

E

G

G

A

P

P

A

G

Q

Q

L

Q

E

E

I

I

N

D

L

L

R

R

H

F

G

A

D

D

P

A

I

L

E

S

L

M

A

A

D

D

Q

N

V

V

F

M

M

T

F

F

K

R

R

Y

T

V

I

I

R

K

E

E

A

V

A

A

L

L

K

E

H

E

G

G

I

A

Y

R

A

A

T

S

F

F

M

M

A

P

K

K

P

P

M

F

Q

G

G

Q

Q

H

P

P

G

G

S

S

A

A

M

M

H

H

I

T

H

H

Q

M

S

S

V

L

I

F

D

E

I

G

E

D

T

V

G

-

R

-

N

N

I

A

F

F

S

H

S

S

P

A

D

D

G

D

-

P

-

L

S

Q

P

L

S

S

K

E

E

V

F

G

F

K

A

S

F

F

I

I

G

A

G

G

M

I

Q

L

H

E

Y

H

V

A

P

C

K

E

T

I

L

S

S

A

M

V

M

T

A

N

P

Q

Y

W

V

V

N

N

S

S

Y

Y

R

K

R

R

L

L

T

V

P

Q

D

G

M

G

S

E

A

A

P

P

V

T

N

A

T

A

A

S

W

W

G

G

Y

A

D

A

N

N

R

R

T

S

T

A

A

L

F

V

R

R

I

V

P

P

V

M

S

Y

D

T

P

P

-

H

-

K

-

T

V

S

A

S

R

R

I

V

E

E

N

V

R

R

L

S

P

P

S

D

S

S

D

A

A

C

N

N

P

P

Y

Y

L

L

A

T

L

F

A

A

A

V

S

L

L

L

G

A

C

A

G

G

Y

L

L

R

G

G

I

V

M

E

E
x
K

D

G

L

Y

Q

V

P

L

T

G

P

P

P

Q

S

A

E

E

D

D

T

N

A

V

N

W

E

D

-

L

-

T

-

P

-

E

-

R

-

A

-

M

G

G

E

Y

I

R

D

E

L

L

P

P

R

S

G

S

L

L

L

D

E

S

A

A

I

L

S

R

L

A

L

M

E

E

S

A

E

S

P

E

S

L

L

V

A

A

E

E

V

A

F

L

S

G

A

E

E

H

F

V

I

F

A

D

I

F

Y

F

A

L

G

R

V

N

K

K

R

R

G

T

E

E

F

W

E

A

T

N

F

Y

M

R

Q

S

V

H

I

V

S

T

P

P

W

Y

E

E

-

L

R

R

E

T

F

Y

L

L

L

S

L

L

K363 (≠ E403) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

7tf6A Glutamine synthetase (see paper)
30% identity, 77% coverage: 103:472/478 of query aligns to 63:432/438 of 7tf6A

query
sites
7tf6A

D

E

S

S

D

D

I

M

K

Y

L

L

V

H

P

P

D

D

L

L

S

D

T

T

I

W

S

V

I

I

V

F

P

P

W

W

E

-

S

-

D

G

P

K

T

V

A

A

Q

R

V

L

I

I

C

C

D

D

I

V

V

Y

G

K

S

T

Q

D

G

G

E

T

Q

P

I

F

S

E

Y

G

T

D

P

P

R

R

N

A

V

N

L

L

K

K

R

R

V

V

L

L

D

K

L

E

Y

M

R

E

Q

D

K

L

G

G

W

F

K

T

P

D

V

F

-

N

V

L

A

G

P

P

E
|
E

I

P

E
|
E

F

F

Y

F

L

L

V

F

A

K

Q

L

N

D

D

E

D

-

P

-

D

-

Y

-

P

-

L

-

R

-

P

-

K

K

G

G

R

E

S

P

G

T

R

L

S

E

I

L

L

N

G

D

Q

G

G

G

Y

Y

-

F

S

D

I

L

A

A

G

P

I

T

N

D

E

L

F

G

D

E

E

N

L

C

I

R

D

R

D

D

I

I

Y

V

H

L

F

E

S

L

E

E

K

D

Q

M

G

G

L

F

E

D

I

I

D

E

T

A

L

S

I

H

H

H

E
|
E

E

V

G

A

P

P

A

G

Q

Q

L

H

E
|
E

I

I

N

D

L

F

R

K

H

Y

G

A

D

D

P

A

I

V

E

T

L

A

A

C

D

D

Q

N

V

I

F

Q

M

T

F

F

K

K

R

L

T

V

I

V

R

K

E

T

A

I

A

A

L

R

K

K

H

H

G

N

I

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

L

Q

F

G
|
G

Q

V

P
x
N

G
|
G

S

S

A

G

M

M

H
|
H

I

F

H

N

Q

V

S

S

V

L

I

F

D

-

I

-

E

K

T

G

G

K

R

E

N

N

I

A

F

F

S

F

S

D

P

P

D

N

G

T

S

E

P

M

-

G

-

L

S

T

K

E

E

T

F

A

F

Y

A

Q

F

F

I

T

G

A

G

G

M

V

Q

L

H

K

Y

N

V

A

P

R

K

G

T

F

L

T

S

A

M

V

M

C

A

N

P

P

Y

L

V

V

N

N

S

S

Y

Y

R

K

R
|
R

L

L

T

V

P

P

D
x
G

M
x
Y

S
x
E

A

A

P

P

V

C

N

Y

T

I

A

A

W

W

G

S

Y

G

D

K

N

N

R
|
R

T

S

T

P

A

L

F

I

R

R

I

V

P

P

V

S

S

S

D

R

P

G

V

L

A

S

R

T

R

R

I

I

E
|
E

N

V

R

R

L

S

P

V

S

D

S

P

D

A

A

A

N

N

P

P

Y

Y

L

M

A

A

L

L

A

A

S

I

L

L

G

E

C

A

G

G

Y

L

L

D

G

G

I

I

M

K

E

N

D

K

L

L

Q

K

P

V

T

P

P

E

P

P

S

V

E

N

D

Q

T

N

A

I

N
x
Y

E

E

-

M

-

N

-

R

-

E

-

R

-

E

-

A

-

V

G

G

E

I

I

Q

D

D

L

L

P

P

R

S

G

T

L

L

L

Y

E

T

A

A

I

L

S

K

L

A

L

M

E

R

S

E

E

N

P

E

S

V

L

I

A

K

E

K

V

A

F

L

S

G

A

N

E

H

F

I

I

Y

A

N

I

Q

Y

F

A

I

G

N

V

S

K

K

R

S

G

I

E

E

F

W

E

D

T
x
Y

F

Y

M

R

Q

T

V

Q

I

V

S

S

P

E

W

W

E

E

R

R

E

D

binding glutamine: E128 (= E169), E183 (= E232), G235 (= G284), H239 (= H288), R292 (= R341), E298 (≠ S347)
binding magnesium ion: E126 (= E167), E128 (= E169), E183 (= E232), E190 (= E239), H239 (= H288), E327 (= E376)
binding : G232 (= G281), N234 (≠ P283), G296 (≠ D345), Y297 (≠ M346), R310 (= R359), Y367 (≠ N416), Y421 (≠ T461)

Sites not aligning to the query:

binding : 58, 60, 433, 437

7tdvC Glutamine synthetase (see paper)
30% identity, 77% coverage: 103:472/478 of query aligns to 64:437/443 of 7tdvC

query
sites
7tdvC

D

E

S

S

D

D

I

M

K

Y

L

L

V

H

P

P

D

D

L

L

S

D

T

T

I

W

S

V

I

I

V

F

P

P

W

W

E

T

S

A

D

G

-

Q

-

G

P

K

T

V

A

A

Q

R

V

L

I

I

C

C

D

D

I

V

V

Y

G

K

S

T

Q

D

G

G

E

T

Q

P

I

F

S

E

Y

G

T

D

P

P

R

R

N

A

V

N

L

L

K

K

R

R

V

V

L

L

D

K

L

E

Y

M

R

E

Q

D

K

L

G

G

W

F

K

T

P

D

V

F

-

N

V

L

A
x
G

P

P

E
|
E

I

P

E
|
E

F

F

Y

F

L

L

V

F

A

K

Q

L

N

D

D

E

D

-

P

-

D

-

Y

-

P

-

L

-

R

-

P

-

K

K

G

G

R

E

S

P

G

T

R

L

S

E

I

L

L

N

G

D

Q

G

G

G

Y

Y

-

F

S

D

I

L

A

A

G

P

I

T

N

D

E

L

F

G

D

E

E

N

L

C

I

R

D

R

D

D

I

I

Y

V

H

L

F

E

S

L

E

E

K

D

Q

M

G

G

L

F

E

D

I

I

D
x
E

T

A

L

S

I

H

H

H

E
|
E

E

V

G

A

P

P

A

G

Q

Q

L

H

E
|
E

I

I

N
x
D

L
x
F

R
x
K

H
x
Y

G

A

D

D

P

A

I

V

E

T

L

A

A

C

D

D

Q

N

V

I

F

Q

M

T

F

F

K

K

R

L

T

V

I

V

R

K

E

T

A

I

A

A

L

R

K

K

H

H

G

N

I

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

L

Q

F

G

G

Q

V

P

N

G
|
G

S

S

A

G

M

M

H
|
H

I

F

H
x
N

Q
x
V

S
|
S

V

L

I

F

D

-

I

-

E

K

T

G

G

K

R

E

N

N

I

A

F

F

S

F

S

D

P

P

D

N

G

T

S

E

P

M

-

G

-

L

S

T

K

E

E

T

F

A

F

Y

A

Q

F

F

I

T

G

A

G

G

M

V

Q

L

H

K

Y

N

V

A

P

R

K

G

T

F

L

T

S

A

M

V

M

C

A

N

P

P

Y

L

V

V

N

N

S

S

Y

Y

R

K

R
|
R

L

L

T

V

P

P

D

G

M

Y

S
x
E

A

A

P

P

V

C

N

Y

T

I

A

A

W

W

G

S

Y

G

D

K

N

N

R
|
R

T

S

T

P

A

L

F

I

R
|
R

I

V

P

P

V

S

S

S

D

R

P

G

V

L

A
x
S

R

T

R
|
R

I

I

E
|
E

N

V

R

R

L

S

P

V

S

D

S

P

D

A

A

A

N

N

P

P

Y

Y

L

M

A

A

L

L

A

A

S

I

L

L

G

E

C

A

G

G

Y

L

L

D

G

G

I

I

M

K

E

N

D

K

L

L

Q

K

P

V

T

P

P

E

P

P

S

V

E

N

D

Q

T

N

A

I

N

Y

E

E

-

M

-

N

-

R

-

E

-

R

-

E

-

A

-

V

G

G

E

I

I

Q

D

D

L

L

P

P

R

S

G

T

L

L

L

Y

E

T

A

A

I

L

S

K

L

A

L

M

E

R

S

E

E

N

P

E

S

V

L

I

A

K

E

K

V

A

F

L

S

G

A

N

E

H

F

I

I

Y

A

N

I

Q

Y

F

A

I

G

N

V

S

K

K

R

S

G

I

E

E

F

W

E

D

T

Y

F

Y

M

R

Q

T

V

Q

I

V

S

S

P

E

W

W

E

E

R

R

E

D

binding adenosine-5'-diphosphate: G129 (≠ A165), E131 (= E167), E183 (≠ D227), D197 (≠ N241), F198 (≠ L242), K199 (≠ R243), Y200 (≠ H244), N246 (≠ H290), V247 (≠ Q291), S248 (= S292), R320 (= R364), S328 (≠ A372), R330 (= R374)
binding magnesium ion: E131 (= E167), E131 (= E167), E133 (= E169), E188 (= E232), E195 (= E239), E195 (= E239), H244 (= H288), E332 (= E376)
binding l-methionine-s-sulfoximine phosphate: E131 (= E167), E133 (= E169), E188 (= E232), E195 (= E239), G240 (= G284), H244 (= H288), R297 (= R341), E303 (≠ S347), R315 (= R359)

5dm3C Crystal structure of glutamine synthetase from chromohalobacter salexigens dsm 3043(csal_0679, target efi-550015) with bound adp
29% identity, 76% coverage: 110:470/478 of query aligns to 58:390/396 of 5dm3C

query
sites
5dm3C

P

P

D

D

L

L

S

A

T

T

I

L

S

R

I

C

V

V

P

P

W

W

E

-

S

L

D

E

P

G

T

T

A

A

Q

M

V

V

I

L

C

C

D

D

I

L

V

L

G

D

S

H

Q

R

G

T

E

H

-

A

Q

E

I

V

S

P

Y

H

T

A

P

P

R

R

N

A

V

I

L

L

K

K

R

R

V

Q

L

L

D

A

L

R

Y

L

R

E

Q

A

K

M

G

G

W

L

K

E

P

A

V

I

V

M

A

A

P

T

E
|
E

I

L

E
|
E

F

F

Y

F

L

L

V

F

A

E

Q

K

N

S

D

L

D

D

P

E

D

-

Y

-

P

-

L

I

R

R

P

K

P

G

K

R

G

F

R

R

S

T

G

T

R

K

S

E

I

-

L

-

G

-

Q

-

G

-

Y

-

S

-

I

-

A

-

G

-

I

-

N

-

E

-

F

-

D

E

E

H

L

V

I

L

D

R

D

P

I

L

Y

R

H

N

F

H

S

L

E

H

K

A

Q

A

G

G

L

I

E

P

I

V

D

E

T

G

L

T

I

K

H

G

E
|
E

E

A

G

G

P

A

A

G

Q

Q

L

E

E
|
E

I

L

N

N

L

I

R
|
R

H
x
C

G

A

D

K

P

A

I

L

E

D

L

T

A

A

D

D

Q

Y

V

H

F

T

M

I

F

A

K

K

R

H

T

A

I

T

R

K

E

E

A

I

A

A

L

W

K

Q

H

Q

G

G

I

R

Y

A

A

V

T

T

F

F

M

L

A

S

K

K

P

W

M

H

Q

H

G

A

Q

H

P

A

G

G

S

S

A

S

M

S

H
|
H

I

I

H
|
H

Q

Q

S
|
S

V

L

I

W

D

-

I

-

E

K

T

Q

G

G

R

L

N

P

I

A

F

F

S

H

S

D

P

E

D

R

G

D

S

A

-

L

-

G

P

M

S

S

K

A

E

L

F

M

F

K

A

H

F

Y

I

L

G

A

G

G

M

L

Q

L

H

K

Y

Y

V

A

P

P

K

D

T

Y

L

T

S

Y

M

F

M

L

A

A

P

P

Y

Y

V

L

N

N

S

S

Y

Y

R

K

R

R

L

F

T

-

P

-

D

-

M

Q

S

F

A

A

P

P

V

T

N

R

T

T

A

V

W

W

G

S

Y

V

D

D

N

N

R
|
R

T

T

T

A

A

G

F

F

R

R

I

L

P

C

V

A

S

E

D

G

P

T

V

R

A

A

R

V

R
|
R

I

I

E
|
E

N

C

R
|
R

L

I

P

G

S

G

S

S

D

D

A

L

N

N

P

P

Y

Y

L

L

A

A

L

M

A

A

A

G

S

Q

L

L

G

A

C

A

G

G

Y

I

L

K

G

G

I

I

M

E

E

E

D

C

L

L

Q

A

P

L

T

P

P

P

S

A

E

S

D

G

T

L

A

-

N

-

E

-

G

-

E

-

I

-

D

-

L

I

P

P

R

Q

G

N

L

L

L

R

E

D

A

A

I

M

S

E

L

A

L

L

E

R

S

G

E

S

P

T

S

M

L

L

A

R

E

E

V

A

F

M

S

G

A

E

E

D

F

V

I

V

A

D

I

H

Y

Y

A

V

G

R

V

A

K

A

R

E

G

V

E

E

F

L

E

E

T

D

F

F

M

Q

Q

R

V

V

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V

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S

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D

W

Y

E

E

active site: E115 (= E167), E117 (= E169), E162 (= E232), E169 (= E239), H218 (= H288), R286 (= R359), E303 (= E376), R305 (= R378)
binding adenosine-5'-diphosphate: R173 (= R243), C174 (≠ H244), H220 (= H290), S222 (= S292), R301 (= R374)

Query Sequence

>WP_012706904.1 NCBI__GCF_000018545.1:WP_012706904.1
MSSKRSVGQQTAKVSKSSGVPLALHAPRGVKTWKEAVDWLKIRGIEDIECITPDLAGVPR
GKMMPTSKFTSNTSLALPSAIYRHTISGEYPEETGQFRYDSRDSDIKLVPDLSTISIVPW
ESDPTAQVICDIVGSQGEQISYTPRNVLKRVLDLYRQKGWKPVVAPEIEFYLVAQNDDPD
YPLRPPKGRSGRSILGGQGYSIAGINEFDELIDDIYHFSEKQGLEIDTLIHEEGPAQLEI
NLRHGDPIELADQVFMFKRTIREAALKHGIYATFMAKPMQGQPGSAMHIHQSVIDIETGR
NIFSSPDGSPSKEFFAFIGGMQHYVPKTLSMMAPYVNSYRRLTPDMSAPVNTAWGYDNRT
TAFRIPVSDPVARRIENRLPSSDANPYLALAASLGCGYLGIMEDLQPTPPSEDTANEGEI
DLPRGLLEAISLLESEPSLAEVFSAEFIAIYAGVKRGEFETFMQVISPWEREFLLLNV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory