SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012707018.1 NCBI__GCF_000018545.1:WP_012707018.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5wjsA Crystal structure of oxidoreductase (short chain dehydrogenase/reductase family) from burkholderia thailandensis complexed with nadh
47% identity, 97% coverage: 6:254/256 of query aligns to 9:256/258 of 5wjsA

query
sites
5wjsA

S

A

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Y

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P

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A

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N

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N

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F

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D

M

M

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x
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K

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W

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G

active site: G27 (= G24), S152 (= S150), Y162 (≠ I160), Y165 (= Y163), K169 (= K167)
binding 1,4-dihydronicotinamide adenine dinucleotide: G23 (= G20), T26 (≠ S23), I28 (= I25), D47 (= D44), L48 (≠ I45), D73 (= D71), L74 (= L72), N100 (= N98), A102 (= A100), L150 (≠ F148), G151 (≠ S149), S152 (= S150), K169 (= K167), P195 (= P193), G196 (= G194), W197 (≠ M195), V198 (≠ I196), K202 (≠ R200)

7wwxA Crystal structure of herbaspirillum huttiense l-arabinose 1- dehydrogenase (NAD bound form) (see paper)
47% identity, 97% coverage: 6:254/256 of query aligns to 4:252/254 of 7wwxA

query
sites
7wwxA

S

A

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N

F

F

P

P

D

S

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D

G

R

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G

R

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G

G

G

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x
T

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I

G

G

A

A

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N

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D

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D

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H

P

K

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L

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M

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F

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A

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G

G

binding nicotinamide-adenine-dinucleotide: G18 (= G20), T21 (≠ S23), I23 (= I25), D42 (= D44), I43 (= I45), C68 (≠ A70), D69 (= D71), L70 (= L72), N96 (= N98), A98 (= A100), F146 (= F148), S147 (= S149), S148 (= S150), Y161 (= Y163), K165 (= K167), P191 (= P193), G192 (= G194), W193 (≠ M195), V194 (≠ I196), R198 (= R200)

9gwwA Crystal structure of sulfoquinovose-1-dehydrogenase from pseudomonas putida in complex with sulfoquinovose substrate (sulfo-ed pathway) (see paper)
43% identity, 98% coverage: 6:255/256 of query aligns to 10:254/257 of 9gwwA

query
sites
9gwwA

S

T

Q

H

F

Y

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P

D

S

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A

D

D

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E

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x
R

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G

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Q

R

D

R

A

L

A

W

G

L

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T

-

E

E

E

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G

A

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F

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I

N

D

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Q

Q

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K

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A

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C

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D

D

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A

G

G

V

L

binding 6-deoxy-6-sulfo-beta-D-glucopyranose: D102 (= D102), R104 (= R104), S150 (= S150), I151 (= I151), R155 (≠ L155), Y163 (= Y163), G194 (= G194), Q201 (= Q201)

9gwvA Crystal structure of sulfoquinovose-1-dehydrogenase from pseudomonas putida in complex with NAD+ (sulfo-ed pathway) (see paper)
42% identity, 98% coverage: 6:255/256 of query aligns to 8:250/253 of 9gwvA

query
sites
9gwvA

S

T

Q

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G

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I

G

G

A

R

A

A

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E

G

A

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D

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x
L

A

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A

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A

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T

G

L

L

A

A

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A

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-

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P

-

E

L

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x
C

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D

L
x
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E

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K

N

A

A

C

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A

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A

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E

A

R

A

T

L

W

G

G

P

A

I

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G

D

V

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L

L

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I

N

N

N
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N

A

A

A
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A

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D

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R

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H

P

A

L

L

E

A

A

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K

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E

E

S

Y

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W

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D

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M

A

Q

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T

N

N

L

L

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R

H

H

F

A

F

F

L

A

S

A

Q

Q

A

A

V

V

A

A

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P

H

G

M

M

R

A

Q

R

A

R

G

G

G

S

G

G

S

A

I

I

N

N

F
x
M

S

G

S

S

I

I

A

S

F

W

K

M

L

R

N

G

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A

C

Y
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Y

A

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T

T

A

A

K
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K

A

A

G

A

I

L

I

N

G

G

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M

T

T

K

R

S

T

L

L

A

A

G

R

K

E

L

L

G

G

P

G

D

Q

N

G

I

I

R

R

V

I

N

N

A

S

I

L

L

V

P
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P

G
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G

M

A

I
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I

V

R

T

T

E

E

R

D

Q

P

R

A

R

G

L

L

W

-

L

-

T

-

E

E

E

A

T

A

I

S

A

Q

G

A

M

F

Q

I

N

D

R

Q

Q

Q

C

M

L

L

K

K

R

F

M

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L

L

V

D

A

A

E

S

D

D

L

C

V

A

G

R

P

L

C

A

L

L

F

F

L

L

A

A

S

S

D

D

C

D

S

S

G

R

A

G

M

C

T

T

A

G

Q

Q

T

N

M

F

I

V

D

D

G

A

G

G

V

L

binding nicotinamide-adenine-dinucleotide: G22 (= G20), S25 (= S23), I27 (= I25), D46 (= D44), L47 (≠ I45), C68 (≠ A70), D69 (= D71), V70 (≠ L72), N96 (= N98), A98 (= A100), M146 (≠ F148), Y161 (= Y163), K165 (= K167), P191 (= P193), G192 (= G194), I194 (= I196)

5itvA Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
36% identity, 96% coverage: 10:254/256 of query aligns to 4:251/255 of 5itvA

query
sites
5itvA

D

N

L

L

R

T

D

D

R

K

G

T

V

V

L

L

V

I

S

T

G
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G

G

G

G

A

S
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S

G
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G

I
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I

G

G

A

Y

A

A

L

A

V

V

E

Q

G

A

F

F

L

L

R

G

Q

Q

G

Q

A

A

R

N

V

V

A

V

F

V

I

A

D
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D

I
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I

A

D

E

E

D

A

S

Q

S

G

R

E

T

A

L

M

A

V

D

R

K

K

L

-

A

-

E

E

T

N

G

N

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D

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R

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L

E

H

F

F

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H

Q

A
x
T

D

D

L
x
I

A

T

D

D

V

E

E

A

Q

A

A

C

K

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N

H

A

A

A

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D

E

A

S

A

A

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V

A

H

A

T

L

F

G

G

P

G

I

L

G

D

V

V

L

L

V

I

N

N

N
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N

A

A

A
x
G

R

I

D

E

D

I

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Q

A

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P

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I

E

H

A

E

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M

T

E

K

L

E

S

S

D

W

W

D

N

D

K

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L

L

A

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V

N

N

L

L

R

T

H

G

F

M

F

F

F

L

L

M

S

S

Q

K

A

H

V

A

A

L

P

K

H

H

M

M

R

L

Q

A

A

A

G

G

G

K

G

G

S

N

I

I

N

N

F
x
T

S

C

S
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S

I

V

A

G

F

G

K

L

L

V

N

A

M

W

P

P

E

D

I

I

P

P

A

A

Y
|
Y

A

N

T

A

A

S

K
|
K

A

G

G

G

I

V

I

L

G

Q

L

L

T

T

K

K

S

S

L

M

A

A

G

V

K

D

L

Y

G

A

P

K

D

H

N

Q

I

I

R

R

V

V

N

N

A

C

I

V

L

C

P
|
P

G
|
G

M
x
I

I
|
I

V

D

T

T

E

P

R

L

Q

N

R

E

R

K

L

S

W

F

L

L

-

E

-

N

T

N

E

E

E

G

T

T

I

L

A

E

G

E

M

I

Q

K

N

K

R

E

Q

K

C

A

-

K

-

V

-

N

-

P

L

L

K

L

R

R

M

L

L

G

V

K

A

P

E

E

D

E

L

I

V

A

G

N

P

V

C

M

L

L

F

F

L

L

A

A

S

S

D

D

C

L

S

S

G

S

A

Y

M

M

T

T

A

G

Q

S

T

A

M

I

I

T

I

A

D

D

G

G

active site: G18 (= G24), S141 (= S150), Y154 (= Y163), K158 (= K167)
binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G20), S17 (= S23), G18 (= G24), I19 (= I25), D38 (= D44), I39 (= I45), T61 (≠ A70), I63 (≠ L72), N89 (= N98), G91 (≠ A100), T139 (≠ F148), S141 (= S150), Y154 (= Y163), K158 (= K167), P184 (= P193), G185 (= G194), I186 (≠ M195), I187 (= I196)

5itvD Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
35% identity, 96% coverage: 10:254/256 of query aligns to 4:223/227 of 5itvD

query
sites
5itvD

D

N

L

L

R

T

D

D

R

K

G

T

V

V

L

L

V

I

S

T

G
|
G

G

G

G

A

S
|
S

G
|
G

I
|
I

G

G

A

Y

A

A

L

A

V

V

E

Q

G

A

F

F

L

L

R

G

Q

Q

G

Q

A

A

R

N

V

V

A

V

F

V

I

A

D
|
D

I
|
I

A

D

E

E

D

A

S

Q

S

G

R

E

T

A

L

M

A

V

D

R

K

K

L

-

A

-

E

E

T

N

G

N

Q

D

R

R

P

L

E

H

F

F

I

V

H

Q

A
x
T

D
|
D

L
x
I

A

T

D

D

V

E

E

A

Q

A

A

C

K

Q

N

H

A

A

A

V

D

E

A

S

A

A

V

V

A

H

A

T

L

F

G

G

P

G

I

L

G

D

V

V

L

L

V

I

N

N

N
|
N

A

A

A

G

R

I

D

E

D

I

R

V

Q

A

P

P

L

I

E

H

A

E

V

M

T

E

K

L

E

S

S

D

W

W

D

N

D

K

S

V

L

L

A

Q

V

V

N

N

L

L

R

T

H

G

F

M

F

F

F

L

L

M

S

S

Q

K

A

H

V

A

A

L

P

K

H

H

M

M

R

L

Q

A

A

A

G

G

G

K

G

G

S

N

I

I

N

N

F
x
T

S

C

S
|
S

I

V

A

G

F

G

K

L

L

V

N

A

M

W

P

P

E

D

I

I

P

P

A

A

Y
|
Y

A

N

T

A

A

S

K
|
K

A

G

G

G

I

V

I

L

G

Q

L

L

T

T

K

K

S

S

L

M

A

A

G

V

K

D

L

Y

G

A

P

K

D

H

N

Q

I

I

R

R

V

V

N

N

A

C

I

V

L

C

P
|
P

G
|
G

M

I

I
|
I

V

D

T

T

E

L

R

R

Q

L

R

G

R

K

L

-

W

-

L

-

T

-

E

-

T

-

I

-

A

-

G

-

M

-

Q

-

N

-

R

-

Q

-

C

-

L

-

K

-

R

-

M

-

L

-

V

-

A

P

E

E

D

E

L

I

V

A

G

N

P

V

C

M

L

L

F

F

L

L

A

A

S

S

D

D

C

L

S

S

G

S

A

Y

M

M

T

T

A

G

Q

S

T

A

M

I

I

T

I

A

D

D

G

G

active site: G18 (= G24), S141 (= S150), Y154 (= Y163), K158 (= K167)
binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G20), S17 (= S23), G18 (= G24), I19 (= I25), D38 (= D44), I39 (= I45), T61 (≠ A70), D62 (= D71), I63 (≠ L72), N89 (= N98), T139 (≠ F148), S141 (= S150), Y154 (= Y163), K158 (= K167), P184 (= P193), G185 (= G194), I187 (= I196)

8cxaA Crystal structure of 3-oxoacyl-[acyl-carrier-protein] reductase from mycobacterium smegmatis with bound NAD
37% identity, 96% coverage: 11:255/256 of query aligns to 3:248/251 of 8cxaA

query
sites
8cxaA

L

L

R

A

D

N

R

R

G

V

V

A

L

I

V

I

S

T

G
|
G

G

A

S
x
Q

G
|
G

I
|
I

G

G

A

R

A

I

L

Y

V

A

E

E

G

R

F

F

L

A

R

E

Q

E

G

G

A

A

R

A

V

V

A

V

F

V

I

A

D
|
D

I

I

A

N

E

E

D

P

S

K

S

A

R

T

T

E

L

V

A

A

D

S

K

S

L

I

A

T

A

S

E

-

T

L

G

G

Q

G

R

R

P

A

E

I

F

A

I

A

H

A

A

V

D

D

L
x
V

A

S

D

D

V

V

E

E

Q

S

A

V

K

N

N

R

A

M

A

V

D

D

A

S

A

A

V

V

A

E

A

A

L

F

G

G

P

T

I

V

G

D

V

I

L

L

V

V

N

N

N
|
N

A

A

A
|
A

-

I

-

F

-
x
S

R

T

D

L

D

K

R

M

Q

K

P

P

L

F

E

E

A

E

V

I

T

S

K

G

E

D

S

E

W

W

D

D

D

K

S

L

L

F

A

A

V

V

N

N

L

A

R

K

H

G

F

T

F

F

F

L

L

C

S

C

Q

Q

A

A

V

V

A

S

P

P

H

V

M

M

R

R

Q

K

A

N

G

Q

G

Y

G

G

S

R

I

I

N

N

F
x
I

S
|
S

I

S

A

V

F

V

K

V

L

T

N

G

M

R

P

P

E

N

I

Y

P

A

A

H

Y
|
Y

A

I

T

A

A

S

K
|
K

A

G

G

A

I

V

I

W

G

A

L

L

T

T

K

H

S

A

L

L

A

A

G

T

K

E

L

M

G

G

P

T

D

D

N

G

I

I

R

T

V

V

N

N

A

T

I

V

L

S

P
|
P

G
x
H

M

G

I
|
I

V

I

T

T

E

E

R
x
I

Q

P

R

R

R

E

L

T

W

-

L

I

T

S

E

E

T

G

I

W

A

R

G

R

M

N

Q

L

N

E

R

E

Q

Q

C

A

L

L

K

K

R

R

M

K

L

G

V

D

A

A

E

S

D

D

L

L

V

V

G

G

P

I

C

L

L

L

F

Y

L

L

A

A

S

S

D

D

C

E

S

S

G

K

A

F

M

M

T

T

A

G

Q

Q

T

T

M

V

I

A

I

L

D

D

G

A

G

G

V

L

binding nicotinamide-adenine-dinucleotide: G12 (= G20), Q15 (≠ S23), G16 (= G24), I17 (= I25), D36 (= D44), V63 (≠ L72), N89 (= N98), A91 (= A100), S94 (vs. gap), I142 (≠ F148), S143 (= S149), S144 (= S150), Y157 (= Y163), K161 (= K167), P187 (= P193), H188 (≠ G194), I190 (= I196), I194 (≠ R200)

3uf0A Crystal structure of a putative NAD(p) dependent gluconate 5- dehydrogenase from beutenbergia cavernae(efi target efi-502044) with bound NADP (low occupancy)
36% identity, 95% coverage: 11:254/256 of query aligns to 5:245/249 of 3uf0A

query
sites
3uf0A

L

L

R

A

D

G

R

R

G

T

V

A

L

V

V

V

S

T

G
|
G

G

A

G

G

S
|
S

G
|
G

I
|
I

G

G

A

R

A

A

L

I

V

A

E

H

G

G

F

Y

L

A

R

R

Q

A

G

G

A

A

R

H

V

V

A

L

F

A

I

W

D

G

I

-

A
x
R

E

T

D

D

S

G

S

V

R

K

T

E

L

V

A

A

D

D

K

E

L

I

A

A

A

-

E

D

T

G

G

G

Q

G

R

S

P

A

E

E

F

A

I

V

H

V

A

A

D
|
D

L
|
L

A

A

D

D

V

L

E

E

Q

G

A

A

K

A

N

N

A

V

A

A

D

E

A

E

A

-

V

L

A

A

L

T

G

R

P

R

I

V

G

D

V

V

L

L

V

V

N

N

N
|
N

A

A

A
x
G

R
x
I

D

I

D

A

R

R

Q

A

P

P

L

A

E

E

A

E

V

V

T

S

K

L

E

G

S

R

W

W

D

R

D

E

S

V

L

L

A

T

V

V

N

N

L

L

R

D

H

A

F

A

F

W

F

V

L

L

S

S

Q

R

A

S

V

F

A

G

P

T

H

A

M

M

R

L

Q

A

A

H

G

G

G

S

G

G

S

R

I

I

I

V

N

T

F
x
I

S
x
A

S
|
S

I

M

A

L

F

S

K

F

L

Q

N

G

M

G

P

R

E

N

I
x
V

P

A

A

A

Y
|
Y

A

A

T

A

A

S

K
|
K

A

H

G

A

I

V

G

G

L

L

T

T

K

R

S

A

L

L

A

A

G

S

K

E

L

W

G

A

P

G

D

R

N

G

I

V

R

G

V

V

N

N

A

A

I

L

L

A

P
|
P

G
|
G

M

Y

I
x
V

V

V

T
|
T

E

A

R
x
N

Q
x
T

R

A

L

L

W

R

L

A

T

D

E

D

E

E

T

R

I

A

A

A

G

E

M

I

Q

T

N

A

R

R

Q

I

C

P

L

A

K

G

R

R

M

W

L

A

V

T

A

P

E

E

D

D

L

M

V

V

G

G

P

P

C

A

L

V

F

F

L

L

A

A

S

S

D

D

C

A

S

A

G

S

A

Y

M

V

T

H

A

G

Q

Q

T

V

M

L

I

A

I

V

D

D

G

G

active site: G18 (= G24), S141 (= S150), V151 (≠ I160), Y154 (= Y163), K158 (= K167)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G14 (= G20), S17 (= S23), G18 (= G24), I19 (= I25), R39 (≠ A46), D63 (= D71), L64 (= L72), N89 (= N98), G91 (≠ A100), I92 (≠ R101), I139 (≠ F148), A140 (≠ S149), S141 (= S150), Y154 (= Y163), K158 (= K167), P184 (= P193), G185 (= G194), V187 (≠ I196), T189 (= T198), N191 (≠ R200), T192 (≠ Q201)

P08074 Carbonyl reductase [NADPH] 2; Adipocyte protein P27; AP27; Lung carbonyl reductase; LCR; NADPH-dependent carbonyl reductase 2; EC 1.1.1.184 from Mus musculus (Mouse) (see 2 papers)
36% identity, 93% coverage: 17:254/256 of query aligns to 11:240/244 of P08074

query
sites
P08074

L
|
L

V
|
V

S
x
T

G
|
G

G
x
A

G
|
G

S
x
K

G
|
G

I
|
I

G
|
G

A
x
R

A
x
D

L
x
T

V
|
V

E
x
K

G
x
A

F
x
L

L
x
H

R
x
A

Q
x
S

G
|
G

A
|
A

R
x
K

V
|
V

A
x
V

F
x
A

I
x
V

D

-

I

-

A

-

E

-

D

-

S

-

S
x
T

R
|
R

T

T

L

N

A

S

D

D

-

L

-

V

K

S

L

L

A

A

A

K

E

E

T

C

-

P

G

G

Q

I

R

E

P

P

E

-

F

-

I

V

H

C

A

V

D

D

L

L

A

G

D

D

V

W

E

D

Q

-

A

-

K

-

N

-

A

A

A

T

D

E

A

K

A

A

V

L

A

G

L

I

G

G

P

P

I

V

G

D

V

L

L

L

V

V

N

N

A

A

R

L

D

V

D

I

R

M

Q

Q

P

P

L

F

E

L

A

E

V

V

T

T

K

K

E

E

S

A

W

F

D

D

D

R

S

S

L

F

A

S

V

V

N

N

L

L

R

R

H

S

F

V

F

F

F

Q

L

V

S

S

Q

Q

A

M

V

V

A

A

P

R

H

D

M

M

R

I

Q

N

A

R

G

G

-

V

G

P

G

G

S

S

I

I

I

V

N

N

F

V

S

S

I

M

A

V

F

A

K

H

L

V

N

T

M

F

P

P

E

N

I

L

P

I

A

T

Y

Y

A

S

T

S

A

T

K

K

A

G

G

A

I

M

I

T

G

M

L

L

T

T

K

K

S

A

L

M

A

A

G

M

K

E

L

L

G

G

P

P

D

H

N

K

I

I

R

R

V

V

N

N

A

S

I

V

L

N

P

P

G

T

M

V

I

V

V

L

T

T

E

D

R

M

Q

G

R

K

L

V

W

S

L

A

T

D

E

P

E

E

T

F

I

A

A

R

G

K

M

L

Q

K

N

E

R

R

Q

H

C

P

L

L

K

R

R

K

M

F

L

A

V

E

A

V

E

E

D

D

L

V

V

V

G

N

P

S

C

I

L

L

F

F

L

L

A

L

S

S

D

D

C

R

S

S

G

A

A

S

M

T

T

S

A

G

Q

G

T

G

M

I

I

L

I

V

D

D

G

A

G

G

11:39 (vs. 17:51, 34% identical) binding NADP(+)
T38 (≠ S50) mutation to R: Converts the coenzyme specificity from NADP to NAD.

1cydA Carbonyl reductase complexed with NADPH and 2-propanol (see paper)
36% identity, 97% coverage: 7:254/256 of query aligns to 2:238/242 of 1cydA

query
sites
1cydA

Q

N

F

F

P

S

D

G

L

L

R

R

D

-

R

-

G

-

V

A

L

L

V

V

S

T

G
|
G

G

A

G
|
G

S
x
K

G
|
G

I
|
I

G

G

A

R

A

D

L

T

V

V

E

K

G

A

F

L

L

H

R

A

Q

S

G

G

A

A

R

K

V

V

A

V

F

A

I

V

D

-

I

-

A

-

E

-

D

-

S

-

S

T

R
|
R

T
|
T

L

N

A

S

D

D

-

L

-

V

K

S

L

L

A

A

A

K

E

E

T

C

-

P

G

G

Q

I

R

E

P

P

E

-

F

-

I

V

H

C

A

V

D
|
D

L
|
L

A

G

D

D

V

W

E

D

Q

-

A

-

K

-

N

-

A

A

A

T

D

E

A

K

A

A

V

L

A

G

L

I

G

G

P

P

I

V

G

D

V

L

L

L

V

V

N

N

N
|
N

A

A

A
|
A

R

L

D

V

D

I

R

M

Q

Q

P

P

L

F

E

L

A

E

V

V

T

T

K

K

E

E

S

A

W

F

D

D

D

R

S

S

L

F

A

S

V
|
V

N

N

L

L

R

R

H

S

F

V

F

F

F

Q

L

V

S

S

Q

Q

A

M

V

V

A

A

P

R

H

D

M

M

R

I

Q

N

A

R

G

G

-

V

G

P

G

G

S

S

I

I

I

V

N

N

F
x
V

S

S

S
|
S

I

M

A

V

F

A

K

H

L

V

N

T

M

F

P

P

E

N

I
x
L

P

I

A

T

Y
|
Y

A

S

T

S

A

T

K
|
K

A

G

G

A

I

M

I

T

G

M

L

L

T

T

K

K

S

A

L

M

A

A

G

M

K

E

L

L

G

G

P

P

D

H

N

K

I

I

R

R

V

V

N

N

A

S

I

V

L

N

P
|
P

G
x
T

M
x
V

I
x
V

V

L

T
|
T

E

D

R
x
M

Q
x
G

R

K

L
x
V

W

S

L

A

T

D

E

P

E

E

T

F

I

A

A

R

G

K

M

L

Q

K

N

E

R

R

Q

H

C

P

L

L

K

R

R

K

M

F

L

A

V

E

A

V

E

E

D

D

L

V

V

V

G

N

P

S

C

I

L

L

F

F

L

L

A

L

S

S

D

D

C

R

S

S

G

A

A

S

M

T

T

S

A

G

Q

G

T

G

M

I

I

L

I

V

D

D

G

A

G

G

active site: G16 (= G24), S134 (= S150), L144 (≠ I160), Y147 (= Y163), K151 (= K167)
binding isopropyl alcohol: S134 (= S150), Y147 (= Y163), V188 (≠ L204)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G12 (= G20), G14 (= G22), K15 (≠ S23), I17 (= I25), R37 (= R51), T38 (= T52), D58 (= D71), L59 (= L72), N81 (= N98), A83 (= A100), V104 (= V121), V132 (≠ F148), S134 (= S150), Y147 (= Y163), K151 (= K167), P177 (= P193), T178 (≠ G194), V179 (≠ M195), V180 (≠ I196), T182 (= T198), M184 (≠ R200), G185 (≠ Q201)

7do7A Crystal structure of azotobacter vinelandii l-rhamnose 1- dehydrogenase(NAD and l-rhamnose bound-form) (see paper)
36% identity, 96% coverage: 11:256/256 of query aligns to 3:252/256 of 7do7A

query
sites
7do7A

L

L

R

I

D

D

R

K

G

T

V

V

L

I

V

V

S

T

G
|
G

G

A

G

S

S
x
R

G
|
G

I
|
I

G

G

A

R

A

A

L

A

V

A

E

R

G

E

F

C

L

A

R

R

Q

Q

G

G

A

A

R

R

V

V

A

V

F

I

I

G

D

H

I
x
S

A

G

E

S

D

D

S

E

S

G

R

R

T

A

L

G

A

A

D

L

K

S

L

L

A

A

A

E

E

E

T

I

-

A

-

F

G

G

Q

G

R

T

P

A

E

I

F

A

I

V

H

G

A

A

D
|
D

L
x
A

A

A

D

D

V

L

E

D

Q

S

A

G

K

E

N

K

A

L

A

V

D

A

A

A

V

V

A

E

A

A

L

F

G

G

P

S

I

V

G

D

V

V

L

L

V

V

N

N

N
|
N

A
|
A

A
x
G

R
x
I

D

C

D

P

R
x
F

Q

H

P

S

L

F

E

L

A

D

V

M

T

P

K

R

E

E

S

L

W

Y

D

L

D

K

S

T

L

V

A

G

V

T

N

N

L

L

R

N

H

G

F

A

F

Y

F

F

L

T

S

V

Q

Q

A

A

V

A

A

A

P

R

H

R

M

M

R

K

Q

E

A

Q

G

G

-

R

G

G

S

A

I

I

N

A

F
x
V

S
|
S

I

I

A
x
S

F

A

K

L

L

V

N

G

M

G

P

A

E

M

I
x
Q

P

T

A

H

Y
|
Y

A

T

T

P

A

T

K
|
K

A

A

G

G

I

L

G

S

L

L

T

M

K

Q

S

S

L

C

A

A

G

I

K

A

L

L

G

G

P

P

D

Y

N

G

I

I

R

R

V

C

N

N

A

A

I

V

L

L

P
|
P

G
|
G

M

T

I
|
I

V

A

T
|
T

E

D

R
x
I

Q
x
N

R

K

R

E

L

D

W

L

L

S

T

D

E

L

E

E

T

K

I

R

A

E

G

R

M

M

Q

T

N

S

R

R

Q

V

C

P

L

L

K

G

R

R

M

L

L

G

V

E

A

P

E

D

D

D

L

L

V

A

G

G

P

P

C

I

L

V

F

F

L

L

A

A

S

S

D
|
D

C
x
M

S

A

G
x
R

A

Y

M

V

T

T

A

G

Q

A

T

S

M

L

I

L

I

V

D

D

G

G

V

L

F

F

active site: G16 (= G24), S146 (= S150), Y159 (= Y163)
binding nicotinamide-adenine-dinucleotide: G12 (= G20), R15 (≠ S23), G16 (= G24), I17 (= I25), S37 (≠ I45), D66 (= D71), A67 (≠ L72), N93 (= N98), A94 (= A99), G95 (≠ A100), I96 (≠ R101), V144 (≠ F148), S145 (= S149), S146 (= S150), Y159 (= Y163), K163 (= K167), P189 (= P193), G190 (= G194), I192 (= I196), T194 (= T198), I196 (≠ R200)
binding beta-L-rhamnopyranose: F99 (≠ R104), S146 (= S150), S148 (≠ A152), Q156 (≠ I160), Y159 (= Y163), N197 (≠ Q201), D235 (= D239), M236 (≠ C240), R238 (≠ G242)

7b81A Crystal structure of azotobacter vinelandii l-rhamnose 1-dehydrogenase (NAD bound-form) (see paper)
36% identity, 96% coverage: 11:256/256 of query aligns to 3:252/256 of 7b81A

query
sites
7b81A

L

L

R

I

D

D

R

K

G

T

V

V

L

I

V

V

S

T

G
|
G

G

A

G
x
S

S
x
R

G
|
G

I
|
I

G

G

A

R

A

A

L

A

V

A

E

R

G

E

F

C

L

A

R

R

Q

Q

G

G

A

A

R

R

V

V

A

V

F

I

I

G

D

H

I

S

A

G

E

S

D

D

S

E

S

G

R

R

T

A

L

G

A

A

D

L

K

S

L

L

A

A

A

E

E

E

T

I

-

A

-

F

G

G

Q

G

R

T

P

A

E

I

F

A

I

V

H

G

A

A

D
|
D

L
x
A

A

A

D

D

V

L

E

D

Q

S

A

G

K

E

N

K

A

L

A

V

D

A

A

A

V

V

A

E

A

A

L

F

G

G

P

S

I

V

G

D

V

V

L

L

V

V

N

N

N
|
N

A
|
A

A
x
G

R
x
I

D

C

D

P

R

F

Q

H

P

S

L

F

E

L

A

D

V

M

T

P

K

R

E

E

S

L

W

Y

D

L

D

K

S

T

L

V

A

G

V
x
T

N

N

L

L

R

N

H

G

F

A

F

Y

F

F

L

T

S

V

Q

Q

A

A

V

A

A

A

P

R

H

R

M

M

R

K

Q

E

A

Q

G

G

-

R

G

G

S

A

I

I

N

A

F
x
V

S

S

S
|
S

I

I

A

S

F

A

K

L

L

V

N

G

M

G

P

A

E

M

I

Q

P

T

A

H

Y
|
Y

A

T

T

P

A

T

K
|
K

A

A

G

G

I

L

G

S

L

L

T

M

K

Q

S

S

L

C

A

A

G

I

K

A

L

L

G

G

P

P

D

Y

N

G

I

I

R

R

V

C

N

N

A

A

I

V

L

L

P
|
P

G
|
G

M

T

I
|
I

V

A

T
|
T

E

D

R
x
I

Q

N

R

K

R

E

L

D

W

L

L

S

T

D

E

L

E

E

T

K

I

R

A

E

G

R

M

M

Q

T

N

S

R

R

Q

V

C

P

L

L

K

G

R

R

M

L

L

G

V

E

A

P

E

D

D

D

L

L

V

A

G

G

P

P

C

I

L

V

F

F

L

L

A

A

S

S

D

D

C

M

S

A

G

R

A

Y

M

V

T

T

A

G

Q

A

T

S

M

L

I

L

I

V

D

D

G

G

V

L

F

F

active site: G16 (= G24), S146 (= S150), Y159 (= Y163)
binding nicotinamide-adenine-dinucleotide: G12 (= G20), S14 (≠ G22), R15 (≠ S23), I17 (= I25), D66 (= D71), A67 (≠ L72), N93 (= N98), A94 (= A99), G95 (≠ A100), I96 (≠ R101), T116 (≠ V121), V144 (≠ F148), S146 (= S150), Y159 (= Y163), K163 (= K167), P189 (= P193), G190 (= G194), I192 (= I196), T194 (= T198), I196 (≠ R200)

C1DMX5 L-rhamnose 1-dehydrogenase (NAD(P)(+)); RhaDH; AvLRA1; EC 1.1.1.378 from Azotobacter vinelandii (strain DJ / ATCC BAA-1303) (see paper)
36% identity, 96% coverage: 11:256/256 of query aligns to 3:252/256 of C1DMX5

query
sites
C1DMX5

L

L

R

I

D

D

R

K

G

T

V

V

L

I

V

V

S

T

G
|
G

G

A

G
x
S

S
x
R

G

G

I
|
I

G

G

A

R

A

A

L

A

V

A

E

R

G

E

F

C

L

A

R

R

Q

Q

G

G

A

A

R

R

V

V

A

V

F

I

I

G

D

H

I
x
S

A

G

E

S

D

D

S

E

S

G

R

R

T

A

L

G

A

A

D

L

K

S

L

L

A

A

A

E

E

E

T

I

-

A

-

F

G

G

Q

G

R

T

P

A

E

I

F

A

I

V

H

G

A

A

D
|
D

L
x
A

A

A

D

D

V

L

E

D

Q

S

A

G

K

E

N

K

A

L

A

V

D

A

A

A

V

V

A

E

A

A

L

F

G

G

P

S

I

V

G

D

V

V

L

L

V

V

N

N

N
|
N

A

A

A

G

R

I

D

C

D

P

R
x
F

Q

H

P

S

L

F

E

L

A

D

V

M

T

P

K

R

E

E

S

L

W

Y

D

L

D

K

S

T

L

V

A

G

V

T

N

N

L

L

R

N

H

G

F

A

F

Y

F

F

L

T

S

V

Q

Q

A

A

V

A

A

A

P

R

H

R

M

M

R

K

Q

E

A

Q

G

G

-

R

G

G

S

A

I

I

N

A

F

V

S

S

S
|
S

I

I

A
x
S

F

A

K

L

L

V

N

G

M

G

P

A

E

M

I
x
Q

P

T

A

H

Y
|
Y

A

T

T

P

A

T

K
|
K

A

A

G

G

I

L

G

S

L

L

T

M

K

Q

S

S

L

C

A

A

G

I

K

A

L

L

G

G

P

P

D

Y

N

G

I

I

R

R

V

C

N

N

A

A

I

V

L

L

P

P

G

G

M
x
T

I
|
I

V

A

T

T

E

D

R
x
I

Q
x
N

R

K

R

E

L
x
D

W

L

L

S

T

D

E

L

E

E

T

K

I

R

A

E

G

R

M

M

Q

T

N

S

R

R

Q

V

C

P

L

L

K

G

R

R

M

L

L

G

V

E

A

P

E

D

D

D

L

L

V

A

G

G

P

P

C

I

L

V

F

F

L

L

A

A

S

S

D

D

C

M

S

A

G

R

A

Y

M

V

T

T

A

G

Q

A

T

S

M

L

I

L

I

V

D

D

G

G

V

L

F

F

G12 (= G20) binding NADP(+)
S14 (≠ G22) binding NADP(+)
R15 (≠ S23) binding NADP(+); mutation to T: Increases specificity toward NAD(+). Shows a strong decrease in catalytic efficiency with NADP(+).
I17 (= I25) binding NADP(+)
S37 (≠ I45) binding NADP(+); mutation to H: Increases specificity toward NAD(+). Shows a strong decrease in catalytic efficiency with NADP(+) and an increase in catalytic efficiency with NAD(+).
D66 (= D71) binding NADP(+)
A67 (≠ L72) binding NADP(+)
N93 (= N98) binding NADP(+)
F99 (≠ R104) mutation F->A,Y: Shows a strong decrease in catalytic efficiency with L-rhamnose, L-lyxose and L-mannose.
S146 (= S150) binding beta-L-rhamnose
S148 (≠ A152) binding beta-L-rhamnose
Q156 (≠ I160) binding beta-L-rhamnose; mutation to A: Almost loss of activity with L-rhamnose as substrate.
Y159 (= Y163) binding beta-L-rhamnose; binding NADP(+)
K163 (= K167) binding NADP(+)
T191 (≠ M195) binding beta-L-rhamnose; mutation to F: Retains 4% of wild-type activity with L-rhamnose as substrate.
I192 (= I196) binding NADP(+)
I196 (≠ R200) mutation to A: Shows a strong decrease in catalytic efficiency with L-rhamnose as substrate, but does not affect catalytic efficiency with L-lyxose and L-mannose.
N197 (≠ Q201) binding beta-L-rhamnose
D200 (≠ L204) mutation to A: Retains 16% of wild-type activity with L-rhamnose as substrate.; mutation to H: Retains 22% of wild-type activity with L-rhamnose as substrate.

7krmC Putative fabg bound to nadh from acinetobacter baumannii
36% identity, 96% coverage: 11:256/256 of query aligns to 3:242/244 of 7krmC

query
sites
7krmC

L

L

R

Q

D

G

R

K

G

V

V

A

L

L

V

I

S

T

G
|
G

G

A

S
|
S

-

E

-

R

G
|
G

I
|
I

G

G

A

R

A

A

L

T

V

A

E

E

G

I

F

F

L

A

R

Q

Q

Q

G

G

A

A

R

K

V

V

A

I

F

I

I

V

D
|
D

I
x
L

A

D

E

L

D

A

S

Q

S

S

R

Q

T

N

L

A

A

A

D

K

K

A

L

L

A

-

E

-

T

-

G

G

Q

E

R

G

P

H

E

M

F

G

I

L

H

A

A
|
A

D
x
N

L
x
V

A

A

D

N

V

E

E

E

Q

Q

A

V

K

K

N

A

A

A

A

V

D

E

A

Q

A

A

V

L

A

Q

A

H

L

Y

G

G

P

K

I

I

G

D

V

I

L

L

V

I

N

N

N
|
N

A

A

A

G

R

I

D

-

R

T

Q

Q

P

P

L

I

E

K

A

T

-

L

-

D

V

I

T

Q

K

R

E

S

S

D

W

Y

D

D

D

R

S

V

L

L

A

D

V
|
V

N

S

L

L

R

R

H

G

F

T

F

L

F

I

L

M

S

S

Q

Q

A

A

V

V

A

I

P

P

H

S

M

M

R

K

Q

A

A

N

G

G

S

S

I

I

I

V

N

C

F

L

S

S

S
|
S

I

V

A

S

F

A

K

Q

L

R

N

G

M

G

P

G

E

I

I

F

-

G

P

P

A

H

Y
|
Y

A

S

T

A

A

A

K
|
K

A

A

G

G

I

V

I

L

G

G

L

L

T

A

K

K

S

A

L

M

A

A

G

R

K

E

L

F

G

G

P

G

D

D

N

Q

I

I

R

R

V

V

N

N

A

S

I

L

L

T

P

P

G

G

M

L

I
|
I

V

Q

T
|
T

E

D

R

M

Q

N

-

D

R

R

L

-

W

-

L

-

T

-

E

H

E

D

T

I

I

L

A

A

G

G

M

I

Q

P

N

-

R

-

Q

-

C

-

L

L

K

G

R

R

M

L

L

G

V

K

A

A

E

Q

D

D

L

V

V

A

G

N

P

A

C

A

L

L

F

F

L

L

A

A

S

S

D

D

C

L

S

S

G

A

A

Y

M

L

T

T

A

G

Q

V

T

T

M

L

I

D

I

V

D

N

G

G

V

M

F

L

active site: G18 (= G24), S140 (= S150), Y155 (= Y163)
binding nicotinamide-adenine-dinucleotide: G12 (= G20), S15 (= S23), G18 (= G24), I19 (= I25), D38 (= D44), L39 (≠ I45), A60 (= A70), N61 (≠ D71), V62 (≠ L72), N88 (= N98), V111 (= V121), S140 (= S150), Y155 (= Y163), K159 (= K167), I188 (= I196), T190 (= T198)

7wbcA Hydroxysteroid dehydrogenase wild-type complexed with NAD+ and (4s)-2- 2-methyl-2,4-pentanediol
36% identity, 95% coverage: 11:254/256 of query aligns to 3:245/250 of 7wbcA

query
sites
7wbcA

L

L

R

R

D

G

R

K

G

T

V

A

L

V

V

V

S

T

G
|
G

G

G

G

A

S

G

G
|
G

I
|
I

G

G

A

R

A

A

L

V

V

T

E

R

G

V

F

F

L

V

R

R

Q

E

G

G

A

A

R

R

V

V

A

L

F

F

I

V

D
|
D

I
x
V

A

D

E

D

D

D

S

R

S

G

R

R

T

A

L

L

A

E

D

S

K

E

L

L

A

T

A

G

E

A

T

G

G

G

Q

E

R

-

P

A

E

K

F

F

I

L

H

Q

A
|
A

D
|
D

L
x
I

A

S

D

R

V

R

E

E

Q

S

A

A

K

D

N

Q

A

I

A

R

D

D

A

A

V

V

A

A

L

F

G

G

P

G

I

I

G

D

V

I

L

L

V

V

N

N

N
|
N

A

A

A

H

R

A

D

S

D

R

R

Q

Q

A

P

L

L

L

E

V

A

E

V

H

T

T

K

P

E

E

S

M

W

F

D

E

D

L

S

S

L

F

A

G

V

T

N

G

L

F

R
x
Y

H
x
P

F

T

F

V

F
x
H

L

L

S

M

Q

Q

A

A

V

C

A

Y

P

P

H

Q

M

L

R

K

Q

Q

A

A

G

-

G

R

G

G

S

S

I

V

N

N

F
|
F

S

G

S
|
S

I

G

A

S

F

A

K

L

L

D

N

G

M

M

P

P

E

T

I

Q

P

T

A

S

Y
|
Y

A

A

T

A

A

A

K
|
K

A

E

G

A

I

I

I

R

G

A

L

V

T

S

K

R

S

V

L

A

A

A

G

N

K

E

L

W

G

A

P

A

D

D

N

G

I

I

R

R

V

V

N

N

A

V

I

V

L

C

P
|
P

G
x
F

M
x
A

I

A

V
x
T

T

E

E
x
G

R
x
V

Q

Q

R

A

R

W

L

Q

W

Q

L

A

T

F

E

P

E

D

T

R

I

A

A

A

G

A

M

A

Q

A

N

A

R

K

Q

V

C

P

L

L

K

Q

R

R

M

I

L

G

V

D

A

P

E

E

D

T

L

D

V

I

G

A

P

P

-

V

C

V

L

V

F

F

L

L

A

A

S

S

D

D

C

D

S

S

G

K

A

Y

M

M

T

T

A

G

Q

Q

T

T

M

L

I

M

I

A

D

D

G

G

binding calcium ion: Y115 (≠ R124), P116 (≠ H125), H119 (≠ F128)
binding nicotinamide-adenine-dinucleotide: G12 (= G20), G16 (= G24), I17 (= I25), D36 (= D44), V37 (≠ I45), A61 (= A70), D62 (= D71), I63 (≠ L72), N89 (= N98), F138 (= F148), S140 (= S150), Y153 (= Y163), K157 (= K167), P183 (= P193), F184 (≠ G194), A185 (≠ M195), T187 (≠ V197), G189 (≠ E199), V190 (≠ R200)

5u9pB Crystal structure of a gluconate 5-dehydrogenase from burkholderia cenocepacia j2315 in complex with NADP and tartrate
35% identity, 100% coverage: 1:255/256 of query aligns to 5:257/261 of 5u9pB

query
sites
5u9pB

M

M

T

T

P

H

P

A

S

L

S

D

Q

R

F

F

P

R

D

-

L

L

R

D

D

G

R

R

G

R

V

A

L

L

V

I

S

T

G
|
G

G

S

G

G

S
x
R

G
|
G

I
|
I

G

G

A

L

A

T

L

L

V

A

E

R

G

G

F

L

L

A

R

E

Q

A

G

G

A

A

R

A

V

I

A

V

F

I

I

N

D

D

I
x
R

A

N

E

E

D

E

S

K

S

A

R

A

T

T

L

L

A

A

D

R

K

R

L

F

A

R

A

D

E

E

T

-

G

G

Q

F

R

A

P

A

E

D

F

H

I

A

H

V

A

F

D
|
D

L
x
V

A

A

D

E

V

H

E

A

Q

Q

A

V

K

R

N

A

A

A

A

I

D

D

A

E

A

F

V

E

A

A

A

R

L

V

G

G

P

A

I

I

G

D

V

I

L

L

V

V

N

N

N
|
N

A
|
A

A

G

R

I

D

Q

D

R

R

R

Q

A

P

P

L

L

E

D

A

A

V

F

T

E

K

P

E

D

S

D

W

W

D

H

D

A

S

L

L

M

A

R

V

V

N

N

L

L

R

D

H

G

F

V

F

F

F

N

L

V

S

A

Q

Q

A

A

V

V

A

A

P

R

H

H

M

M

R

I

Q

A

A

R

G

G

G

H

G

G

S

K

I

I

N

N

F
x
I

S

C

S
|
S

I

V

A

Q

F

S

K

E

L

L

N

A

M

R

P

P

E

T

I

I

P

A

A

P

Y
|
Y

A

A

T

A

A

T

K
|
K

A

G

G

A

I

V

I

R

G

M

L

L

T

T

K

K

S

G

L

M

A

C

G

A

K

D

L

W

G

A

P

R

D

Y

N

G

I

I

R

Q

V

A

N

N

A

G

I

L

L

A

P
|
P

G

G

M

Y

I
x
F

V

E

T
|
T

E

E

R
x
L

Q
x
N

R

R

R

A

L

L

-

V

-

D

-

A

-

F

-

S

-

D

W

W

L

L

T

C

E

K

E

R

T

T

I

P

A

A

G

G

M

-

Q

-

N

-

R

-

Q

-

C

-

L

-

K

-

R

R

M

W

L

G

V

Q

A

V

E

D

D

E

L

L

V

C

G

G

P

A

C

A

L

I

F

F

L

L

A

A

S

S

D

A

C

A

S

S

G

D

A

F

M

V

T

N

A

G

Q

Q

T

T

M

L

I

F

I

V

D

D

G

G

V

L

active site: G27 (= G24), S152 (= S150), Y165 (= Y163), K169 (= K167)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G23 (= G20), R26 (≠ S23), G27 (= G24), I28 (= I25), R48 (≠ I45), D73 (= D71), V74 (≠ L72), N100 (= N98), A101 (= A99), I150 (≠ F148), Y165 (= Y163), K169 (= K167), P195 (= P193), F198 (≠ I196), T200 (= T198), L202 (≠ R200), N203 (≠ Q201)

Q5P5I4 (S)-1-Phenylethanol dehydrogenase; EC 1.1.1.311 from Aromatoleum aromaticum (strain DSM 19018 / LMG 30748 / EbN1) (Azoarcus sp. (strain EbN1)) (see 2 papers)
32% identity, 96% coverage: 11:255/256 of query aligns to 5:246/249 of Q5P5I4

query
sites
Q5P5I4

L

L

R

K

D

D

R

K

G

L

V

A

L

V

V

I

S

T

G

G

G

A

S
x
N

G
|
G

I
|
I

G

G

A

R

A

A

L

I

V

A

E

E

G

R

F

F

L

A

R

V

Q

E

G

G

A

A

R

D

V

I

A

A

F

I

I

A

D
|
D

I

L

-

V

-

P

-

A

-

P

-

E

A

A

E

E

D

A

S

A

S

I

R

R

T

N

L

L

A

-

D

-

K

-

L

-

A

-

E

-

T

-

G

G

Q

R

R

R

P

V

E

L

F

T

I

V

H

K

A
x
C

D
|
D

L
x
V

A

S

D

Q

V

P

E

G

Q

D

A

V

K

E

N

A

A

F

A

G

D

K

A

Q

A

V

V

I

A

S

A

T

L

F

G

G

P

R

I

C

G

D

V

I

L

L

V

V

N

N

N
|
N

A

A

A

G

R

I

D
x
Y

D

P

R

L

Q

I

P

P

L

F

E

D

A

E

V

L

T

T

K

F

E

E

S

Q

W

W

D

K

S

T

L

F

A

E

V

I

N

N

L

V

R

D

H

S

F

G

F

F

F

L

L

M

S

A

Q

K

A

A

V

F

A

V

P

P

H

G

M

M

R

K

Q

R

A

N

G

G

G

W

G

G

S

R

I

I

N

N

F

L

S

T

S

S

I

T

A

T

F

Y

K

W

L

L

N

K

M

I

P

E

E

A

I

Y

P

T

A

H

Y

Y

A

I

T

S

A

T

K
|
K

A

A

G

A

I

N

I

I

G

G

L

F

T

T

K

R

S

A

L

L

A

A

G

S

K

D

L

L

G

G

P

K

D

D

N

G

I

I

R

T

V

V

N

N

A

A

I

I

L

A

P
|
P

G
x
S

M
x
L

I
x
V

V

R

T

T

E

A

R
x
T

Q

T

R

E

R

A

L

S

W

A

L

L

T

S

E

A

E

M

T

F

I

D

A

V

G

L

M

P

Q

N

N

M

R

L

Q

Q

C

A

L

I

K

P

R

R

M

L

L

Q

V

V

A

P

E

L

D

D

L

L

V

T

G

G

P

A

C

A

L

A

F

F

L

L

A

A

S

S

D

D

C

D

S

A

G

S

A

F

M

I

T

T

A

G

Q

Q

T

T

M

L

I

A

I

V

D

D

G

G

V

M

NGI 17:19 (≠ SGI 23:25) binding NAD(+)
D38 (= D44) binding NAD(+)
CDV 61:63 (≠ ADL 70:72) binding NAD(+)
N89 (= N98) binding NAD(+)
Y93 (≠ D102) binding NAD(+)
K158 (= K167) binding NAD(+)
PSLV 184:187 (≠ PGMI 193:196) binding NAD(+)
T191 (≠ R200) binding NAD(+)

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

2ewmB Crystal structure of the (s)-specific 1-phenylethanol dehydrogenase of the denitrifying bacterium strain ebn1 (see paper)
32% identity, 96% coverage: 11:255/256 of query aligns to 3:244/247 of 2ewmB

query
sites
2ewmB

L

L

R

K

D

D

R

K

G

L

V

A

L

V

V

I

S

T

G
|
G

G

G

G

A

S
x
N

G
|
G

I
|
I

G

G

A

R

A

A

L

I

V

A

E

E

G

R

F

F

L

A

R

V

Q

E

G

G

A

A

R

D

V

I

A

A

F

I

I

A

D
|
D

I
x
L

-

V

-

P

-

A

-

P

-

E

A

A

E

E

D

A

S

A

S

I

R

R

T

N

L

L

A

-

D

-

K

-

L

-

A

-

E

-

T

-

G

G

Q

R

R

R

P

V

E

L

F

T

I

V

H

K

A
x
C

D
|
D

L
x
V

A

S

D

Q

V

P

E

G

Q

D

A

V

K

E

N

A

A

F

A

G

D

K

A

Q

A

V

V

I

A

S

A

T

L

F

G

G

P

R

I

C

G

D

V

I

L

L

V

V

N

N

N
|
N

A

A

A

G

R

I

D

Y

D

P

R

L

Q

I

P

P

L

F

E

D

A

E

V

L

T

T

K

F

E

E

S

Q

W

W

D

K

S

T

L

F

A

E

V

I

N

N

L

V

R

D

H

S

F

G

F

F

F

L

L

M

S

A

Q

K

A

A

V

F

A

V

P

P

H

G

M

M

R

K

Q

R

A

N

G

G

G

W

G

G

S

R

I

I

N

N

F

L

S

T

S
|
S

I

T

A

T

F

Y

K

W

L

L

N

K

M

I

P

E

E

A

I
x
Y

P

T

A

H

Y
|
Y

A

I

T

S

A

T

K
|
K

A

A

G

A

I

N

I

I

G

G

L

F

T

T

K

R

S

A

L

L

A

A

G

S

K

D

L

L

G

G

P

K

D

D

N

G

I

I

R

T

V

V

N

N

A

A

I

I

L

A

P
|
P

G
x
S

M
x
L

I
x
V

V

R

T

T

E

A

R
x
T

Q

T

R

E

R

A

L

S

W

A

L

L

T

S

E

A

E

M

T

F

I

D

A

V

G

L

M

P

Q

N

N

M

R

L

Q

Q

C

A

L

I

K

P

R

R

M

L

L

Q

V

V

A

P

E

L

D

D

L

L

V

T

G

G

P

A

C

A

L

A

F

F

L

L

A

A

S

S

D

D

C

D

S

A

G

S

A

F

M

I

T

T

A

G

Q

Q

T

T

M

L

I

A

I

V

D

D

G

G

V

M

active site: G16 (= G24), S139 (= S150), Y149 (≠ I160), Y152 (= Y163), K156 (= K167)
binding nicotinamide-adenine-dinucleotide: G12 (= G20), N15 (≠ S23), G16 (= G24), I17 (= I25), D36 (= D44), L37 (≠ I45), C59 (≠ A70), D60 (= D71), V61 (≠ L72), N87 (= N98), S139 (= S150), Y152 (= Y163), K156 (= K167), P182 (= P193), S183 (≠ G194), L184 (≠ M195), V185 (≠ I196), T189 (≠ R200)

4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
33% identity, 96% coverage: 11:255/256 of query aligns to 3:245/247 of 4jroC

query
sites
4jroC

L

L

R

Q

D

G

R

K

G

V

V

A

L

V

V

V

S

T

G
|
G

G

G

G
x
S

S
x
R

G
|
G

I
|
I

G

G

A

R

A

D

L

I

V

A

E

I

G

N

F

L

L

A

R

K

Q

E

G

G

A

A

R

N

V

I

A

F

F

F

I
x
N

D
x
Y

I
x
N

A
x
G

E
x
S

D

P

S

E

S

A

R

A

T

E

L

E

A

T

D

A

K

K

L

L

A

V

A

A

E

E

T

H

G

G

Q

V

R

E

P

V

E

E

F

A

I

M

H

K

A

A

D
x
N

L
x
V

A

A

D

I

V

A

E

E

Q

D

A

V

K

D

N

A

A

F

D

K

A

Q

A

A

V

I

A

E

A

R

L

F

G

G

P

R

I

V

G

D

V

I

L

L

V

V

N

N

N
|
N

A
|
A

A

G

R
x
I

D

T

D

R

R

D

Q

N

P

L

L

L

E

M

A

R

V

M

T

K

K

E

E

D

S

E

W

W

D

D

S

V

L

I

A

N

V
x
I

N

N

L

L

R

K

H

G

F

T

F

F

F

L

L

C

S

T

Q

K

A

A

V

V

A

S

P

R

H

T

M

M

R

M

Q

K

A

Q

G

R

G

A

G

G

S

K

I

I

N

N

F

M

S

A

S
|
S

I

V

A

V

F

G

K

L

L

I

N

G

M

N

P

A

E

G

I
x
Q

P

A

A

N

Y
|
Y

A

V

T

A

A

S

K
|
K

A

A

G

G

I

V

I

I

G

G

L

L

T

T

K

K

S

T

L

T

A

A

G

R

K

E

L

L

G

A

P

P

D

R

N

G

I

I

R

N

V

V

N

N

A

A

I

V

L

A

P
|
P

G

G

M

F

I
|
I

V

T

T
|
T

E

D

R

M

Q

T

R

D

R

K

L

-

W

-

L

L

T

D

E

E

E

K

T

T

I

K

A

E

G

A

M

M

Q

L

N

A

R

Q

Q

I

C

P

L

L

K

G

R

A

M

Y

L

G

V

T

A

T

E

E

D

D

L

I

V

A

G

N

P

A

C

V

L

L

F

F

L

L

A

A

S

S

D

D

C

A

S

S

G

K

A

Y

M

I

T

T

A

G

Q

Q

T

T

M

L

I

S

I

V

D

D

G

G

V

M

active site: G16 (= G24), S142 (= S150), Q152 (≠ I160), Y155 (= Y163), K159 (= K167)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G20), S14 (≠ G22), R15 (≠ S23), G16 (= G24), I17 (= I25), N35 (≠ I43), Y36 (≠ D44), N37 (≠ I45), G38 (≠ A46), S39 (≠ E47), N63 (≠ D71), V64 (≠ L72), N90 (= N98), A91 (= A99), I93 (≠ R101), I113 (≠ V121), S142 (= S150), Y155 (= Y163), K159 (= K167), P185 (= P193), I188 (= I196), T190 (= T198)

7do6A Crystal structure of azotobacter vinelandii l-rhamnose 1- dehydrogenase(NADP bound-form) (see paper)
36% identity, 96% coverage: 11:256/256 of query aligns to 3:243/247 of 7do6A

query
sites
7do6A

L

L

R

I

D

D

R

K

G

T

V

V

L

I

V

V

S

T

G
|
G

G

A

G
x
S

S
x
R

G
|
G

I
|
I

G

G

A

R

A

A

L

A

V

A

E

R

G

E

F

C

L

A

R

R

Q

Q

G

G

A

A

R

R

V

V

A

V

F

I

I

G

D
x
H

I
x
S

A

G

E

S

D

D

S

E

S
x
G

R

R

T

A

L

G

A

A

D

L

K

S

L

L

A

A

A

E

E

E

T

I

-

A

-

F

G

G

Q

G

R

T

P

A

E

I

F

A

I

V

H

G

A

A

D
|
D

L
x
A

A

A

D

D

V

L

E

D

Q

S

A

G

K

E

N

K

A

L

A

V

D

A

A

A

V

V

A

E

A

A

L

F

G

G

P

S

I

V

G

D

V

V

L

L

V

V

N

N

N
|
N

A
|
A

A
x
G

R
x
I

D

C

D

P

R

F

Q

H

P

S

L

F

E

L

A

D

V

M

T

P

K

R

E

E

S

L

W

Y

D

L

D

K

S

T

L

V

A

G

V
x
T

N

N

L

L

R

N

H

G

F

A

F

Y

F

F

L

T

S

V

Q

Q

A

A

V

A

A

A

P

R

H

R

M

M

R

K

Q

E

A

Q

G

G

-

R

G

G

S

A

I

I

N

A

F

V

S

S

S
|
S

I

I

A

S

F

A

K

L

L

V

N

G

M

G

P

A

E

M

I

Q

P

T

A

H

Y
|
Y

A

T

T

P

A

T

K
|
K

A

A

G

G

I

L

G

S

L

L

T

M

K

Q

S

S

L

C

A

A

G

I

K

A

L

L

G

G

P

P

D

Y

N

G

I

I

R

R

V

C

N

N

A

A

I

V

L

L

P

P

G

G

M

T

I
|
I

-

A

-

D

V

L

T

E

E

K

R

R

Q

E

R

R

R

-

L

-

W

-

L

-

T

-

E

-

T

-

I

-

A

-

G

-

M

M

Q

T

N

S

R

R

Q

V

C

P

L

L

K

G

R

R

M

L

L

G

V

E

A

P

E

D

D

D

L

L

V

A

G

G

P

P

C

I

L

V

F

F

L

L

A

A

S

S

D

D

C

M

S

A

G

R

A

Y

M

V

T

T

A

G

Q

A

T

S

M

L

I

L

I

V

D

D

G

G

V

L

F

F

active site: G16 (= G24), S146 (= S150), Y159 (= Y163)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G20), S14 (≠ G22), R15 (≠ S23), G16 (= G24), I17 (= I25), H36 (≠ D44), S37 (≠ I45), G42 (≠ S50), D66 (= D71), A67 (≠ L72), N93 (= N98), A94 (= A99), G95 (≠ A100), I96 (≠ R101), T116 (≠ V121), S146 (= S150), Y159 (= Y163), K163 (= K167), I192 (= I196)

Query Sequence

>WP_012707018.1 NCBI__GCF_000018545.1:WP_012707018.1
MTPPSSQFPDLRDRGVLVSGGGSGIGAALVEGFLRQGARVAFIDIAEDSSRTLADKLAAE
TGQRPEFIHADLADVEQAKNAADAAVAALGPIGVLVNNAARDDRQPLEAVTKESWDDSLA
VNLRHFFFLSQAVAPHMRQAGGGSIINFSSIAFKLNMPEIPAYATAKAGIIGLTKSLAGK
LGPDNIRVNAILPGMIVTERQRRLWLTEETIAGMQNRQCLKRMLVAEDLVGPCLFLASDC
SGAMTAQTMIIDGGVF

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory