SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012708791.1 NCBI__GCF_000018545.1:WP_012708791.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P0A6F1 Carbamoyl phosphate synthase small chain; Carbamoyl phosphate synthetase glutamine chain; EC 6.3.5.5 from Escherichia coli (strain K12) (see paper)
51% identity, 95% coverage: 13:393/401 of query aligns to 4:378/382 of P0A6F1

query
sites
P0A6F1

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S47 (= S56) binding L-glutamine
G241 (= G255) binding L-glutamine
G243 (= G257) binding L-glutamine
L270 (= L285) binding L-glutamine
Q273 (= Q288) binding L-glutamine
N311 (= N326) binding L-glutamine
G313 (= G328) binding L-glutamine
F314 (= F329) binding L-glutamine

1ce8B Carbamoyl phosphate synthetase from escherichis coli with complexed with the allosteric ligand imp (see paper)
51% identity, 95% coverage: 13:393/401 of query aligns to 3:377/379 of 1ce8B

query
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1ce8B

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active site: C268 (= C284), H352 (= H368), E354 (= E370)
binding L-ornithine: R119 (≠ A133), R122 (= R136)

1c3oB Crystal structure of the carbamoyl phosphate synthetase: small subunit mutant c269s with bound glutamine (see paper)
50% identity, 95% coverage: 13:393/401 of query aligns to 3:377/379 of 1c3oB

query
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1c3oB

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active site: K201 (= K216), S268 (≠ C284), H352 (= H368), E354 (= E370)
binding glutamine: S46 (= S56), N239 (= N254), G240 (= G255), G242 (= G257), S268 (≠ C284), L269 (= L285), N310 (= N326), H311 (= H327), G312 (= G328), F313 (= F329)

Q9LVW7 Carbamoyl phosphate synthase small chain, chloroplastic; Carbamoyl phosphate synthetase glutamine chain; Protein VENOSA 6; EC 6.3.5.5 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
45% identity, 97% coverage: 7:393/401 of query aligns to 53:427/430 of Q9LVW7

query
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Q9LVW7

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T

I

D

S

P

T

S

S

N

N

Y

W

R

R

A

C

A

T

K

K

H

T

L

L

D

A

A

D

W

Y

L

L

K

T

A

E

R

R

G

D

V

I

I

M

G

G

L

V

C

Y

G

D

I

L

D

D

T

T

R

R

A

A

L

I

T

T

A

R

W

R

I

L

R

R

E

E

N

D

G

G

M

S

P

L

N

I

A

G

V

V

I

L

A

S

H

T

D

E

P

Q

S

S

G

K

V

T

F

D

D

D

I

-

Q

-

T

E

L

L

K

L

A

Q

E

M

A

S

K

R

A

S

W

W

S

D

G

-

L

I

V

V

G

G

L

I

D

D

L

L

A

I

K

S

V

D

A

V

T

S

S

C

G

K

Q

S

S

P

Y

Y

R

E

W

W

N

V

E

D

K

K

-

T

-

N

-

A

P

E

W

W

A

D

W

F

N

N

E

-

G

-

H

-

A

-

T

T

L

N

G

S

E

R

T

D

E

G

A

K

A

S

Y

Y

H

K

V

V

V

I

A

A

L

Y

D

D

Y

F

G

G

V

I

K

K

R

Q

N

N

I

I

L

L

R

R

L

R

F

L

A

S

G

S

L

Y

D

G

C

C

R

Q

V

I

T

T

V

V

P

P

A

S

A

T

T

F

S

P

A

A

D

A

E

E

V

A

L

L

A

K

L

M

K

N

P

P

D

D

G

G

I

I

F

L

L

F

S

S

N

N

G

G

P

P

G

G

D

D

P

P

A

S

A

A

T

V

G

-

E

P

Y

Y

A

A

V

V

P

E

V

T

I

V

K

K

N

E

L

L

K

-

S

G

D

K

I

V

P

P

V

V

F

Y

G

G

I

I

C

C

L

M

G

G

H

H

Q

Q

M

L

L

L

A

G

L

Q

A

A

L

L

G

G

A

G

K

K

T

T

E

F

K

K

M

M

H

K

Q

F

G

G

H

H

G

G

A

G

N

N

H

H

P

P

V

V

K

R

D

N

H

N

T

R

T

T

G

G

K

Q

V

V

E

E

I

I

V

S

S

A

M

Q

N

N

H

H

G

N

F

Y

A

A

V

V

D

D

S

P

K

A

S

S

L

L

P

P

E

G

G

G

V

V

E

E

E

V

T

T

H

H

I

V

S

N

L

L

F

N

D

D

G

G

T

S

N

C

C

A

G

G

L

L

R

S

V

F

N

P

G

E

K

M

P

N

V

V

F

M

S

S

V

L

Q

Q

H

Y

H

H

P

P

E

E

A

A

S

S

P

P

G

G

P

P

Q
x
H

D

D

S

S

H

D

Y

N

L

A

F

F

R

R

R

E

F

F

L

I

N

E

L

L

I

M

R

K

E

R

K

S

K

K

H410 (≠ Q376) mutation to Y: In ven6-1; reticulate leaf phenotype.

Q09794 Multifunctional protein ura1; Pyrimidine-specific carbamoyl phosphate synthase-aspartate carbamoyl transferase; CPSase-ATCase; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
36% identity, 96% coverage: 16:398/401 of query aligns to 61:452/2244 of Q09794

query
sites
Q09794

L

L

V

E

L

L

A

E

D

D

G

K

T

S

V

V

I

L

E

Q

G

G

K

Y

G

S

I

F

G

G

A

A

T

D

G

H

K

S

V

V

Q

S

A

G

E

E

V

L

C

V

F

F

N

Q

T

T

A

G

L

M

T

V

G

G

Y

Y

Q

P

E

E

I

S

L

L

T

T

D

D

P

P

S

S

Y

Y

L

R

G

G

Q

Q

I

I

V

L

T

V

F

F

T

T

F

F

P

P

H

T

I

V

G

G

N

N

I

Y

G

G

A

V

N

P

D

D

E

R

D

R

I

I

E

L

D

D

-

E

-

I

-

S

-

G

-

L

-

P

-

K

L

Y

T

F

P

E

A

S

A

N

R

Q

H

I

G

H

A

V

V

A

G

A

V

I

I

I

F

I

K

S

A

S

D

Y

I

S

T

Q

D

N

P

Y

S

S

N

H

Y

F

R

L

A

A

A

H

K

S

H

S

L

L

D

G

A

E

W

W

L

L

K

K

A

E

R

Q

G

G

V

I

I

P

G

G

L

I

C

F

G

G

I

I

D

D

T

T

R

R

A

A

L

L

T

T

A

K

W

K

I

I

R

R

E

D

N

Q

G

G

-

S

-

M

M

L

P

G

N

R

A

L

V

L

I

I

A

Q

H

K

D

D

P

E

S

S

G

P

V

I

F

-

D

N

I

P

Q

S

T

S

L

I

K

T

A

G

E

L

A

G

K

K

A

D

W

W

S

S

-

T

G

A

L

F

V

E

G

D

L

I

D

P

L

W

A

K

K

N

V

P

A

N

T

T

S

E

G

N

Q

L

S

T

Y

S

R

Q

W

V

N

S

E

I

K

K

P

E

W

P

A

K

W

L

N

Y

E

E

G

P

H

H

A

P

T

T

L

T

G

A

E

I

T

K

E

K

A

A

-

D

-

G

-

K

A

I

Y

I

H

R

V

I

V

L

A

V

L

I

D

D

Y

V

G

G

V

M

K

K

R

Y

N

N

I

Q

L

I

R

R

L

C

F

F

A

L

G

N

L

R

D

G

C

V

R

E

V

L

T

L

V

V

P

P

A

W

A

D

T

Y

S

-

A

-

D

-

E

D

V

F

L

T

A

K

L

E

K

T

P

Y

D

D

G

G

I

L

F

F

L

I

S

S

N

N

G

G

P

P

G

G

D

D

P

P

A

S

A

L

T

M

G

-

E

D

Y

L

A

V

V

V

P

D

V

R

I

V

K

K

N

R

L

V

L

L

K

E

S

S

D

K

-

T

I

V

P

P

V

V

F

F

G

G

I

I

C

C

L

F

G

G

H

H

Q

Q

M

I

L

M

A

A

L

R

A

A

L

A

G

G

A

A

K

S

T

T

E

T

K

K

M

M

H

K

Q

F

G

G

H

N

H

R

G

G

A

H

N

N

H

I

P

P

V

C

K

T

D

C

H

M

T

I

T

S

G

G

K

R

V

C

E

Y

I

I

V

T

S

S

M

Q

N

N

H

H

G

G

F

Y

A

A

V

V

D

D

S

A

K

S

S

S

L

L

P

S

E

N

G

G

V

W

E

K

E

E

T

L

H

F

I

V

S

N

L

A

F

N

D

D

G

G

T

S

N

N

C

E

G

G

L

I

R

Y

V

N

N

T

G

E

K

Y

P

P

V

F

F

F

S

S

V

V

Q

Q

H

F

H

H

P

P

E

E

A

S

S

T

P

P

G

G

P

P

Q

R

D

D

S

T

H

E

Y

F

L

L

F

F

R

D

R

V

F

F

L

I

N

D

L

V

I

V

R

K

E

R

K

S

K

A

G

D

E

A

P

K

A

S

L

L

Sites not aligning to the query:

1119 modified: Phosphoserine
1881 modified: Phosphoserine
1885 modified: Phosphoserine

P20054 Multifunctional protein pyr1-3; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2; EC 3.5.2.3 from Dictyostelium discoideum (Social amoeba)
39% identity, 93% coverage: 16:387/401 of query aligns to 11:380/2225 of P20054

query
sites
P20054

L

L

V

V

L

L

A

E

D

D

G

G

T

T

V

K

I

L

E

S

G

G

K

Y

G

S

I

F

G

G

A

S

T

E

G

R

K

S

V

V

Q

A

A

G

E

E

V

C

C

V

F

F

N

S

T

T

A

G

L

M

T

V

G

G

Y

Y

Q

N

E

E

I

S

L

I

T

S

D

D

P

P

S

S

Y

Y

L

T

G

G

Q

Q

I

I

V

L

T

V

F

F

T

S

F

F

P

P

H

L

I

I

G

G

N

N

I

Y

G

G

A

V

-

P

-

S

-

F

N

R

D

E

E

R

D

D

I

P

E

E

D

S

-

G

-

L

-

A

L

V

T

N

P

F

A

E

A

S

R

D

H

K

G

A

A

H

V

V

G

Q

V

A

I

I

F

I

K

C

A

S

D

E

I

Y

T

C

D

D

P

E

-

Y

S

S

N

H

Y

W

R

A

A

A

A

E

K

K

H

S

L

L

D

S

A

E

W

W

L

L

K

K

A

E

R

S

G

N

V

I

I

P

G

G

L

L

C

Y

G

G

I

I

D

D

T

T

R

R

A

A

L

L

T

I

A

T

W

K

I

I

R

R

E

E

N

K

G

G

-

S

M

L

P

K

N

G

A

K

V

V

I

I

A

I

H

G

D

D

P

-

S

-

G

-

V

-

F

F

D

D

I

E

Q

S

T

K

L

L

K

E

A

F

E

E

A

D

K

I

A

N

W

L

S

R

G

N

L

L

V

V

G

-

L

-

D

-

L

-

A

A

K

E

V

V

A

S

T

T

S

K

G

-

Q

-

S

-

Y

-

R

-

W

-

N

E

E

I

K

K

P

E

W

Y

A

K

W

A

N

A

E

E

G

N

H

N

A

K

T

K

L

T

G

G

E

E

T

K

E

R

A

K

A

N

Y

K

H

K

V

V

V

I

A

V

L

L

D

D

Y

C

G

G

V

I

K

K

R

N

N

N

I

Q

L

I

R

R

-

C

-

L

L

L

F

N

A

R

G

G

L

V

D

D

C

L

R

K

V

V

T

V

V

P

V

W

P

D

A

Y

A

D

T

V

S

V

A

A

D

N

E

E

V

S

L

I

A

N

L

-

K

D

P

Y

D

D

G

G

I

V

F

F

L

I

S

S

N

N

G

G

P

P

G

G

D

D

P

P

A

S

A

L

T

C

G

G

E

K

Y

-

A

A

V

I

P

E

V

N

I

I

K

R

N

K

L

V

L

L

K

A

S

L

D

P

I

V

P

A

-

K

-

A

V

V

F

F

G

G

I

V

C

C

L

M

G

G

H

N

Q

Q

M

L

L

L

A

G

L

L

A

A

L

A

G

G

A

A

K

Q

T

T

E

H

K

K

M

M

H

A

Q

F

G

G

H

N

H

R

G

G

A

L

N

N

H

Q

P

P

V

C

K

V

D

D

H

Q

T

I

T

S

G

G

K

R

V

C

E

H

I

I

V

T

S

S

M

Q

N

N

H

H

G

G

F

F

A

V

V

I

D

D

S

S

K

N

S

S

L

L

P

P

E

A

G

G

V

S

-

G

-

W

E

K

E

T

T

Y

H

F

I

I

S

N

L

A

F

N

D

D

G

A

T

S

N

N

C

E

G

G

L

I

R

Y

V

H

N

E

G

S

K

K

P

P

V

W

F

F

S

S

V

V

Q

Q

H

F

H

H

P

P

E

E

A

A

S

M

P

A

G

G

P

P

Q

T

D

D

S

T

H

E

Y

Y

L

L

F

F

R

D

R

N

F

F

L

V

N

D

Sites not aligning to the query:

1 modified: N-acetylmethionine

P08955 Multifunctional protein CAD; Carbamoyl phosphate synthetase 2-aspartate transcarbamylase-dihydroorotase; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2; EC 3.5.2.3 from Mesocricetus auratus (Golden hamster) (see paper)
38% identity, 94% coverage: 14:391/401 of query aligns to 2:359/2225 of P08955

query
sites
P08955

A

A

L

A

L

L

V

V

L

L

A

E

D

D

G

G

T

S

V

V

I

L

E

Q

G

G

K

R

G

P

I

F

G

G

A

A

T

A

G

V

K

S

V

T

Q

A

A

G

E

E

V

V

C

V

F

F

N

Q

T

T

A

G

L

M

T

V

G

G

Y

Y

Q

P

E

E

I

A

L

L

T

T

D

D

P

P

S

S

Y

Y

L

K

G

A

Q

Q

I

I

V

L

T

V

F

L

T

T

F

Y

P

P

H

L

I

I

G

G

N

N

I

Y

G

G

-

I

-

P

A

S

N

D

D

E

E

E

D

D

I

E

E

F

D

G

L

L

T

S

P

K

-

W

-

F

-

E

A

S

R

E

H

N

G

H

A

V

V

A

G

G

V

L

I

V

F

V

K

G

A

E

D

C

I

C

T

P

D

T

P

P

S

S

N

H

Y

W

R

S

A

A

A

T

K

C

H

T

L

L

D

H

A

E

W

W

L

L

K

Q

A

Q

R

H

G

G

V

I

I

P

G

G

L

L

C

Q

G

G

I

V

D

D

T

T

R

R

A

E

L

L

T

T

A

K

W

K

I

L

R

R

E

E

N

Q

G

G

M

S

P

L

N

L

A

G

V

K

I

L

A

V

H

Q

D

S

P

G

S

T

G

E

V

P

F

S

D

T

I

L

Q

P

T

F

L

V

K

D

A

P

E

N

A

A

K

R

A

P

W

L

S

A

G

P

L

E

V

V

G

S

L

I

D

K

L

T

A

P

K

R

V

V

A

F

T

N

S

A

G

G

Q

G

S

A

Y

P

R

R

W

-

N

-

E

-

K

-

P

-

W

-

A

-

W

-

N

-

E

-

G

-

H

-

A

-

T

-

L

-

G

-

E

-

T

-

E

-

A

-

Y

-

H

-

V

I

V

C

A

A

L

L

D

D

Y

C

G

G

V

L

K

K

R

Y

N

N

I

Q

L

I

R

R

L

C

F

L

A

C

G

Q

L

L

D

G

C

A

R

E

V

V

T

T

V

V

P

P

A

W

A

N

T

H

S

E

A

L

D

D

E

-

V

-

L

-

A

S

L

Q

K

K

P

Y

D

D

G

G

I

L

F

F

L

L

S

S

N

N

G

G

P

P

G

G

D

D

P

P

A

A

A

S

T

Y

G

P

E

G

Y

V

A

V

V

A

P

T

V

L

I

N

K

R

N

V

L

L

L

S

K

E

S

P

D

N

-

P

I

R

P

P

V

V

F

F

G

G

I

I

C

C

L

L

G

G

H

H

Q

Q

M

L

L

L

A

A

L

L

A

A

L

I

G

G

A

A

K

K

T

T

E

Y

K

K

M

M

H

R

Q

Y

G

G

H

N

H

R

G

G

A

H

N

N

H

Q

P

P

V

C

K

L

D

L

H

V

T

G

T

T

G

G

K

R

V

C

E

F

I

L

V

T

S

S

M

Q

N

N

H

H

G

G

F

F

A

A

V

V

D

D

S

A

K

D

S

S

L

L

P

P

E

A

G

G

V

W

E

T

E

P

T

L

H

F

I

T

S

N

L

A

F

N

D

D

G

C

T

S

N

N

C

E

G

G

L

I

R

V

V

H

N

D

G

S

K

L

P

P

V

F

F

F

S

S

V

V

Q

Q

H

F

H

H

P

P

E

E

A

H

S

R

P

A

G

G

P

P

Q

S

D

D

S

M

H

E

Y

L

L

L

F

F

R

D

R

V

F

F

L

L

N

E

L

T

I

V

R

R

E

E

Sites not aligning to the query:

1406 modified: Phosphoserine; by PKA; S→A: No effect on enzyme kinetics.; S→D: Increases CPSase activity and reduces sensitivity to feedback inhibition by UTP.

P27708 Multifunctional protein CAD; Carbamoyl phosphate synthetase 2-aspartate transcarbamylase-dihydroorotase; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2; EC 3.5.2.3 from Homo sapiens (Human) (see 7 papers)
36% identity, 94% coverage: 14:391/401 of query aligns to 2:359/2225 of P27708

query
sites
P27708

A
|
A

L

A

L

L

V

V

L

L

A

E

D

D

G

G

T

S

V

V

I

L

E

R

G

G

K

Q

G

P

I

F

G

G

A

A

T

A

G

V

K

S

V

T

Q

A

A

G

E

E

V

V

C

V

F

F

N

Q

T

T

A

G

L
x
M

T

V

G

G

Y

Y

Q

P

E

E

I

A

L

L

T

T

D

D

P

P

S

S

Y

Y

L

K

G

A

Q

Q

I

I

V

L

T

V

F

L

T

T

F

Y

P

P

H

L

I

I

G

G

N

N

I

Y

G

G

A

I

N

P

D

P

E

D

D

E

I

M

E

D

D

E

L

F

T

G

P

L

A

C

A

K

R

W

H

F

G

E

A

S

V

S

G

G

V

I

I

H

F

V

K

A

A

A

D

L

I

V

T

V

-

G

-

E

-

C

-

P

D

T

P

P

S

S

N

H

Y

W

R

S

A

A

A

T

K

R

H

T

L

L

D

H

A

E

W

W

L

L

K

Q

A

Q

R

H

G

G

V

I

I

P

G

G

L

L

C

Q

G

G

I

V

D

D

T

T

R

R

A

E

L

L

T

T

A

K

W

K

I

L

R

R

E

E

N

Q

G

G

M

S

P

L

N

L

A

G

V

K

I

L

A

V

H

Q

D

N

P

G

S

T

G

E

V

P

F

S

D

S

I

L

Q

P

T

F

L

L

K

D

A

P

E

N

A

A

K

R

A

P

W

L

S

V

G

P

L

E

V

V

G

S

L

I

D

K

L

T

A

P

K

R

V

V

A

F

T

N

S

T

G

G

Q

G

S

A

Y

P

R
|
R

W

-

N

-

E

-

K

-

P

-

W

-

A

-

W

-

N

-

E

-

G

-

H

-

A

-

T

-

L

-

G

-

E

-

T

-

E

-

A

-

Y

-

H

-

V

I

V

L

A

A

L

L

D

D

Y

C

G

G

V

L

K

K

R

Y

N

N

I

Q

L

I

R

R

L

C

F

L

A

C

G

Q

L

R

D

G

C

A

R

E

V

V

T

T

V

V

P

P

A

W

A

D

T

H

S

A

A

L

D

D

E

-

V

-

L

-

A

S

L

Q

K

E

P

Y

D

E

G

G

I

L

F

F

L

L

S

S

N

N

G

G

P

P

G

G

D

D

P

P

A

A

A

S

T

Y

G

P

E

S

Y

V

A

V

V

S

P

T

V

L

I

S

K

R

N

V

L

L

L

S

K

E

S

P

D

N

-

P

I

R

P

P

V

V

F

F

G

G

I

I

C

C

L

L

G

G

H

H

Q

Q

M

L

L

L

A

A

L

L

A

A

L

I

G

G

A

A

K

K

T

T

E

Y

K

K

M

M

H

R

Q

Y

G

G

H

N

H

R

G

G

A

H

N

N

H

Q

P

P

V

C

K

L

D

L

H

V

T

G

T

S

G

G

K

R

V

C

E

F

I

L

V

T

S

S

M

Q

N

N

H

H

G

G

F

F

A

A

V

V

D

E

S

T

K

D

S

S

L

L

P

P

E

A

G

D

V

W

E

A

E

P

T

L

H

F

I

T

S

N

L

A

F

N

D

D

G

G

T

S

N

N

C

E

G

G

L

I

R

V

V

H

N

N

G

S

K

L

P

P

V

F

F

F

S

S

V

V

Q

Q

H

F

H

H

P

P

E

E

A

H

S

Q

P

A

G

G

P

P

Q

S

D

D

S

M

H

E

Y

L

L

L

F

F

R

D

R

I

F

F

L

L

N

E

L

T

I

V

R

K

E

E

A2 (= A14) modified: N-acetylalanine
M33 (≠ L45) to R: in DEE50; uncertain significance; dbSNP:rs751610198
R177 (= R184) to Q: in a colorectal cancer sample; somatic mutation; dbSNP:rs374122292

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
456 modified: Phosphothreonine; by MAPK1
735 Y → C: in a colorectal cancer sample; somatic mutation
1406 modified: Phosphoserine; by PKA
1471 binding Zn(2+); binding Zn(2+); H→A: No zinc-binding and no catalytic activity.; H→N: Abolishes dihydroorotase activity.
1473 binding Zn(2+); H→A: No zinc-binding and no catalytic activity.
1475 binding (S)-dihydroorotate
1505 binding (S)-dihydroorotate
1512 D→N: No change in catalytic activity.
1556 binding via carbamate group; binding via carbamate group; modified: N6-carboxylysine
1562 T→A: Abolishes dihydroorotase activity.
1563 F→A: Abolishes dihydroorotase activity.
1590 binding Zn(2+); H→A: Abolishes dihydroorotase activity.; H→N: No catalytic activity.
1613 binding Zn(2+); C→S: Reduces dihydroorotase activity.
1614 binding Zn(2+); H→A: Abolishes dihydroorotase activity.
1637 binding Zn(2+); E→T: Abolishes dihydroorotase activity.
1642 H→N: 11.5% of wild-type catalytic activity.
1661 binding (S)-dihydroorotate
1686 binding Zn(2+); D→N: Abolishes dihydroorotase activity.
1690 binding (S)-dihydroorotate; H→N: 3% of wild-type catalytic activity.
1702 binding (S)-dihydroorotate
1789:2225 natural variant: Missing (in DEE50; uncertain significance)
1859 modified: Phosphoserine; by RPS6KB1 and PKA
1873 modified: Phosphoserine; by PKC; in vitro; S→A: Abolishes PMA-induced Thr-456 phosphorylation.
1900 modified: Phosphoserine

Q18990 Multifunctional protein pyr-1; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2; EC 3.5.2.3 from Caenorhabditis elegans (see 3 papers)
37% identity, 95% coverage: 14:394/401 of query aligns to 3:365/2198 of Q18990

query
sites
Q18990

A

A

L

T

L

L

V

H

L

L

A

E

D

D

G

G

T

S

V

T

I

F

E

V

G

G

K

S

G

I

I

Y

G

G

A

A

T

T

G

K

K

S

V

V

Q

V

A

G

E

E

V

I

C

V

F

F

N

Q

T

T

A

G

L

M

T

V

G

G

Y

Y

Q

V

E

E

I

S

L

L

T

T

D

D

P

P

S

S

Y

Y

L

A

G

K

Q

Q

I

L

V

L

T

T

F

L

T

T

F

Y

P

P

H

L

I

I

G

G

N

N

I

Y

G

G

A

V

N

P

D

S

E

A

D

E

I

I

E

L

D

D

L

Q

-

F

-

K

T

L

P

P

A

A

A

E

-

F

-

E

-

S

-

D

R

R

H

I

G

W

A

P

V

A

G

A

V

L

I

I

F

V

K

E

A

K

D

I

I

C

T

V

D

D

-

G

-

E

P

H

S

S

N

H

Y

W

R

Q

A

A

A

V

K

Q

H

S

L

L

D

S

A

E

W

W

L

L

K

R

A

K

R

A

G

D

V

V

I

P

G

C

L

L

C

S

G

G

I

I

D

D

T

V

R

R

A

Q

L

L

T

V

A

K

W

K

I

I

R

R

E

E

N

T

G

G

M

T

P

M

N

K

A

A

-

K

-

L

V

V

I

I

A

E

H

S

D

D

P

N

S

A

G

Q

V

N

F

F

D

D

I

Y

Q

V

T

D

L

V

K

N

A

A

E

E

A

-

K

-

A

-

W

-

S

-

G

N

L

L

V

V

G

-

L

-

D

-

L

-

A

-

K

-

V

-

A

-

T

-

S

-

G

-

Q

-

S

D

Y

F

R

V

W

S

N

R

E

K

K

E

P

P

W

V

A

V

W

Y

N

G

E

S

G

G

H

D

A

Q

T

T

L

-

G

-

E

-

T

-

E

-

A

-

Y

-

H

-

V

I

V

L

A

A

L

V

D

D

Y

C

G

G

V

L

K

K

R

N

N

N

I

Q

L

I

R

R

L

C

F

L

A

A

G

K

L

R

D

G

C

F

R

R

V

V

T

K

V

V

P

P

A

W

A

N

T

H

S

P

A

I

D

D

E

T

V

-

L

-

A

E

L

S

K

D

P

Y

D

D

G

G

I

L

F

F

L

L

S

S

N

N

G

G

P

P

G

G

D

D

P

P

A

E

A

I

T

C

G

A

E

P

Y

L

A

V

V

D

P

R

V

L

I

A

K

K

N

V

L

I

L

A

K

R

S

G

D

D

I

K

P

P

V

I

F

F

G

G

I

I

C

C

L

L

G

G

H

H

Q

Q

M

I

L

L

A

S

L

R

A

A

L

I

G

G

A

A

K

K

T

T

E

Y

K

K

M

L

H

K

Q

Y

G

G

H

N

H

R

G

G

A

H

N

N

H

Q

P

P

V

C

K

T

D

H

H

Y

T

A

T

T

G

G

K

R

V

C

E

Y

I

I

V

T

S

S

M

Q

N

N

H

H

G

G

F

Y

A

A

V

V

D

D

S

P

K

D

S

S

L

L

P

P

E

A

G

D

V

W

E

K

E

A

T

L

H

F

I

T

S

N

L

E

F

N

D

D

G

K

T

T

N

N

C

E

G

G

L

I

R

V

V

H

N

S

G

S

K

K

P

P

V

F

F

F

S

S

V

V

Q

Q

H

F

H

H

P

P

E

E

A

H

S

T

P

A

G

G

P

P

Q

T

D

D

S

C

H

E

Y

F

L

L

F

F

R

D

R

V

F

F

L

A

N

D

L

S

I

V

R

R

E

Q

K

A

K

K

G

S

Sites not aligning to the query:

1602 H→Q: In cu8; probable loss of dihydroorotase activity. Severe late stage embryonic lethality in 50% of animals. The few surviving mutants have a shorter and thicker pharyngeal isthmus, an abnormal knobbed tail, mild egg-laying defects, moderate fluid accumulation in the coelom and a slower growth. Actin and intermediate filaments are disorganized in the pharynx. Moderate reduction in heparan sulfate levels and increased levels of chondroitin sulfate. In an umps-1 zu456 mutant background, prevents the formation of abnormally enlarged gut granules in embryos. Complete embryonic lethality in a rnst-2 qx245 mutant background.

P07258 Carbamoyl phosphate synthase arginine-specific small chain; CPS; CPSase; CPSase-arg; Arginine-specific carbamoyl phosphate synthetase, glutamine chain; Carbamoyl phosphate synthase A; CPS-A; Glutamine-dependent carbamoyl phosphate synthetase; EC 6.3.5.5 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
36% identity, 95% coverage: 14:393/401 of query aligns to 8:374/411 of P07258

query
sites
P07258

A

A

L

T

L

F

V

C

L

I

A

Q

D

N

G

G

T

P

V

S

I

F

E

E

G

G

K

I

G

S

I

F

G

G

A

A

T

N

G

K

K

S

V

V

Q

A

A

G

E

E

V

T

C

V

F

F

N

T

T

T

A

S

L

L

T

V

G

G

Y

Y

Q

P

E

E

I

S

L

M

T

T

D

D

P

P

S

S

Y

Y

L

R

G

G

Q

Q

I

I

V

L

T

V

F

F

T

T

F

Q

P

P

H

L

I

I

G

G

N

N

I

Y

G

G

-

V

-

P

-

S

-

G

-

E

A

A

N

R

D

D

E

E

-

Y

D

N

I

L

E

L

D

K

L

Y

T

F

P

E

A

S

A

P

R

H

H

I

G

H

A

V

V

V

G

G

V

I

I

V

F

V

K

A

A

E

D

Y

I

A

T

Y

D

Q

P

Y

S

S

N

H

Y

W

R

T

A

A

A

V

K

E

H

S

L

L

D

A

A

Q

W

W

L

C

K

Q

A

R

R

E

G

G

V

V

I

A

G

A

L

I

C

T

G

G

I

V

D

D

T

T

R

R

A

E

L

L

T

V

A

Q

W

Y

I

L

R

R

E

E

N

Q

G

G

M

S

P

S

N

L

A

G

V

R

I

I

A

T

-

L

-

A

-

D

H

H

D

D

P

P

S

-

G

-

V

V

F

P

D

Y

I

V

Q

N

T

P

L

M

K

K

A

T

E

N

A

L

K

-

A

-

W

-

S

-

G

-

L

-

V

-

G

-

L

-

D

-

L

V

A

A

K

Q

V

V

A

T

T

T

S

-

G

-

Q

-

S

-

Y

-

R

-

W

-

N

-

E

K

K

K

P

P

W

F

A

-

W

-

N

-

E

-

G

-

H

H

A

V

T

S

L

A

G

L

E

P

T

G

E

K

A

A

A

K

Y

A

H

N

V

V

V

A

A

L

L

I

D

D

Y

C

G

G

V

V

K

K

R

E

N

N

I

I

L

I

R

R

L

C

F

L

A

V

G

K

L

R

D

G

C

A

R

N

V

V

T

T

V

V

V

F

P

P

A

Y

A

D

T

Y

S

R

A

I

D

Q

E

D

V

V

L

-

A

A

L

S

K

E

P

F

D

D

G

G

I

I

F

F

L

L

S

S

N

N

G

G

P

P

G

G

D

N

P

P

-

E

-

L

-

C

A

Q

A

A

T

T

G

I

E

S

Y

N

A

V

V

R

P

E

V

L

I

L

K

N

N

N

L

P

L

V

K

Y

S

D

D

C

I

I

P

P

V

I

F

F

G

G

I

I

C
|
C

L

L

G

G

H

H

Q

Q

M

L

L

L

A

A

L

L

A

A

L

S

G

G

A

A

K

S

T

T

E

H

K

K

M

L

H

K

Q

Y

G

G

H

N

H

R

G

A

A

H

N

N

H

I

P

P

V

A

K

M

D

D

H

L

T

T

G

G

K

Q

V

C

E

H

I

I

V

T

S

S

M

Q

N

N

H
|
H

G

G

F

Y

A

A

V

V

D

D

S

P

K

E

S

T

L

L

P

P

E

K

G

D

V

Q

E

W

E

K

T

P

H

Y

-

F

I

V

S

N

L

L

F

N

D

D

G

K

T

S

N

N

C

E

G

G

L

M

R

I

V

H

N

L

G

Q

K

R

P

P

V

I

F

F

S

S

V

T

Q

Q

H

F

H
|
H

P

P

E

E

A

A

S

K

P

G

G

G

P

P

Q

L

D

D

S

T

H

A

Y

I

L

L

F

F

R

D

R

K

F

F

L

F

N

D

L

N

I

I

R

E

E

K

K

Y

K

Q

C264 (= C284) mutation to D: Abolishes glutamine-dependent CPSase activity.
H307 (= H327) mutation to D: Abolishes glutamine-dependent CPSase activity.
H349 (= H368) mutation to D: Abolishes glutamine-dependent CPSase activity.

P07259 Multifunctional protein URA2; Pyrimidine-specific carbamoyl phosphate synthase-aspartate carbamoyl transferase; CPSase-ATCase; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
34% identity, 97% coverage: 5:393/401 of query aligns to 13:411/2214 of P07259

query
sites
P07259

P

P

A

M

W

E

T

S

T

T

Q

G

K

D

P

R

T

L

A

V

L

T

L

L

V

E

L

L

A

K

D

D

G

G

T

T

V

V

I

L

E

Q

G

G

K

Y

G

S

I

F

G

G

A

A

T

E

G

K

K

S

V

V

Q

A

A

G

E

E

V

L

C

V

F

F

N

Q

T

T

A

G

L

M

T

V

G

G

Y

Y

Q

P

E

E

I

S

L

V

T

T

D

D

P

P

S

S

Y

Y

L

E

G

G

Q

Q

I

I

V

L

T

V

F

I

T

T

F

Y

P

P

H

L

I

V

G

G

N

N

I

Y

G

G

A

V

-

P

-

D

-

M

-

H

-

L

N

R

D

D

E

E

D

L

I

V

E

E

D

E

L

L

T

P

-

R

-

Y

-

F

P

E

A

S

A

N

R

R

H

I

G

H

A

I

V

A

G

G

V

L

I

V

F

I

K

S

A

H

D

Y

I

T

T

D

D

E

P

Y

S

S

N

H

Y

Y

R

L

A

A

A

K

K

S

H

S

L

L

D

G

A

K

W

W

L

L

K

Q

A

N

R

E

G

G

V

I

I

P

G

A

L

V

C

Y

G

G

I

V

D

D

T

T

R

R

A

S

L

L

T

T

A

K

W

H

I

L

R

R

E

D

N

A

G

G

M

S

P

M

N

L

A

G

V

R

I

L

A

S

H

L

D

E

P

K

S

S

G

G

V

-

F

-

D

S

I

D

Q

R

T

T

L

I

K

-

A

S

E

R

A

S

K

S

A

S

W

W

S

R

G

S

L

-

V

-

G

A

L

F

D

D

L

V

A

P

K

E

-

W

-

V

-

D

-

P

-

N

V

V

A

Q

T

N

S

L

G

V

Q

S

S

K

Y

V

R

S

W

I

N

N

E

E

K

P

-

K

-

L

-

Y

-

V

P

P

W

P

A

A

W

D

N

N

E

K

G

-

H

H

A

I

T

E

L

L

-

Q

-

T

G

G

E

P

T

D

E

G

A

K

A

V

Y

L

H

R

V

I

V

L

A

A

L

I

D

D

Y

V

G

G

V

M

K

K

R

Y

N

N

I

Q

L

I

R

R

L

C

F

F

A

I

G

K

L

R

D

G

C

V

R

E

V

L

T

K

V

V

P

P

-

W

-

N

-

Y

A

D

A

F

T

T

S

K

A

E

D

D

E

Y

V

-

L

-

A

-

L

-

K

-

P

-

D

D

G

G

I

L

F

F

L

I

S

S

N

N

G

G

P

P

G

G

D

D

P

P

A

S

A

V

T

L

G

D

E

D

Y

L

A

S

V

Q

P

R

V

L

I

S

K

N

N

V

L

L

L

E

K

A

S

K

D

K

I

T

P

P

V

V

F

F

G

G

I

I

C

C

L

L

G

G

H

H

Q

Q

M

L

L

I

A

A

L

R

A

A

L

A

G

G

A

A

K

S

T

T

E

L

K

K

M

L

H

K

Q

F

G

G

H

N

H

R

G

G

A

H

N

N

H

I

P

P

V

C

K

T

D

S

H

T

T

I

T

S

G

G

K

R

V

C

E

Y

I

I

V

T

S

S

M

Q

N

N

H

H

G

G

F

F

A

A

V

V

D

D

S

V

K

D

S

T

L

L

P

T

E

S

G

G

V

W

E

K

E

P

T

L

H

F

I

V

S

N

L

A

F

N

D

D

G

D

T

S

N

N

C

E

G

G

L

I

R

Y

V

H

N

S

G

E

K

L

P

P

V

Y

F

F

S

S

V

V

Q

Q

H

F

H

H

P

P

E

E

A

S

S

T

P

P

G

G

P

P

Q

R

D

D

S

T

H

E

Y

F

L

L

F

F

R

D

R

V

F

F

L

I

N

Q

L

A

I

V

R

K

E

E

K

F

K

K

Sites not aligning to the query:

1857 modified: Phosphoserine; by PKA

P05990 Multifunctional protein r; Protein rudimentary; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2; EC 3.5.2.3 from Drosophila melanogaster (Fruit fly) (see 2 papers)
37% identity, 94% coverage: 16:392/401 of query aligns to 8:377/2224 of P05990

query
sites
P05990

L

L

V

A

L

L

A

E

D

D

G

G

T

T

V

V

I

L

E

P

G

G

K

Y

G

S

I

F

G

G

A

Y

T

V

-

P

-

S

-

E

-

N

-

E

G

S

K

K

V

V

Q

G

-

F

-

G

A

G

E

E

V

V

C

V

F

F

N

Q

T

T

A

G

L

M

T

V

G

G

Y

Y

Q

T

E

E

I

A

L

L

T

T

D

D

P

R

S

S

Y

Y

L

S

G

G

Q

Q

I

I

V

L

T

V

F

L

T

T

F

Y

P

P

H

L

I

I

G

G

N

N

I

Y

G

G

-

V

-

P

A

A

N

P

D

D

E

E

D

D

I

E

E

H

D

G

L

L

T

-

P

P

A

L

A

H

R

F

H

E

G

W

A

M

V

K

G

G

V

V

I

V

F

Q

K

A

A

T

D

A

I

L

T

V

-

V

-

G

-

E

-

V

-

A

-

E

D

E

P

A

S

F

N

H

Y

W

R

R

A

K

A

W

K

K

H

T

L

L

D

P

A

D

W

W

L

L

K

K

A

Q

R

H

G

K

V

V

I

P

G

G

L

I

C

Q

G

D

I

I

D

D

T

T

R

R

A

A

L

L

T

T

A

K

W

K

I

L

R

R

E

E

N

Q

G

G

-

S

M

M

P

L

N

G

A

K

V

I

I

V

A

Y

H

E

D

K

P

P

S

P

G

-

V

-

F

-

D

-

I

-

Q

-

T

-

L

-

K

-

A

V

E

E

A

G

K

L

A

P

W

K

S

S

G

S

L

F

V

V

G

D

L

P

D

N

L

V

A

R

K

N

V

L

A

A

T

-

S

-

G

-

Q

-

S

-

Y

-

R

-

W

-

N

-

E

-

K

K

P

E

W

C

A

S

W

V

N

K

E

E

G

R

H

Q

A

V

T

Y

L

G

G

N

E

P

T

N

E

G

A

K

A

G

Y

P

H

R

V

I

V

A

A

I

L

L

D

D

Y

C

G

G

V

L

K

K

R

L

N

N

I

Q

L

L

R

R

L

C

F

L

A

L

G

Q

L

R

D

G

C

A

R

S

V

V

T

T

V

L

P

P

-

W

A

S

A

A

T

R

S

L

A

E

D

D

E

E

V

Q

L

F

A

-

L

-

K

-

P

-

D

D

G

A

I

L

F

F

L

L

S

S

N

N

G

G

P

P

G

G

D

N

P

P

A

E

A

S

T

C

G

D

E

Q

Y

I

A

V

V

Q

P

Q

V

V

I

R

K

K

N

V

L

I

L

E

K

E

S

G

D

Q

I

K

P

P

V

V

F

F

G

G

I

I

C

C

L

L

G

G

H

H

Q

Q

M

L

L

L

A

A

L

K

A

A

L

I

G

G

A

C

K

S

T

T

E

Y

K

K

M

M

H

K

Q

Y

G

G

H

N

H

R

G

G

A

H

N

N

H

L

P

P

V

C

K

L

D

H

H

R

T

A

T

T

G

G

K

R

V

C

E

L

I

M

V

T

S

S

M

Q

N

N

H

H

G

G

F

Y

A

A

V

V

D

D

S

L

K

E

S

Q

L

L

P

P

E

D

G

G

V

W

E

S

E

E

T

L

H

F

I

V

S

N

L

A

F

N

D

D

G

G

T

T

N

N

C

E

G

G

L

I

R

V

V

H

N

A

G

S

K

K

P

P

V

Y

F

F

S

S

V

V

Q

Q

H

F

H

H

P

P

E

E

A

H

S

H

P

A

G

G

P

P

Q

Q

D

D

S

T

H

E

Y

F

L

L

F

F

R

D

R

V

F

F

L

M

N

E

L

S

I

I

R

Q

E

Q

K

K

Sites not aligning to the query:

1167 E→K: Severely diminishes UTP inhibition of CPSase; in Su(b).
1883 modified: Phosphoserine
1885 modified: Phosphoserine
1892 modified: Phosphoserine
1894 modified: Phosphoserine

Q8C196 Carbamoyl-phosphate synthase [ammonia], mitochondrial; Carbamoyl-phosphate synthetase I; CPSase I; EC 6.3.4.16 from Mus musculus (Mouse) (see 2 papers)
35% identity, 97% coverage: 8:394/401 of query aligns to 40:403/1500 of Q8C196

query
sites
Q8C196

T

S

T

V

Q

K

K

A

P
x
K

T

T

A

A

L

H

L

I

V

V

L

L

A

E

D

D

G

G

T

T

V

K

I

M

E

K

G

G

K

Y

G

S

I

F

G

G

A

H

T

P

G

S

K

S

V

V

Q

A

A

G

E

E

V

V

C

V

F

F

N

N

T

T

A

G

L

L

T

G

G

G

Y

Y

Q

P

E

E

I

A

L

L

T

T

D

D

P

P

S

A

Y

Y

L

K

G

G

Q

Q

I

I

V

L

T

T

F

M

T

A

F

N

P

P

H

I

I

I

G

G

N

N

I

G

G

G

A

A

N

P

D

D

E

T

D

T

I

A

E

R

D

D

L

E

T

L

P

G

A

L

A

N

R

K

H

Y

-

M

-

E

-

S

-

D

-

G

-

I

G

K

A

V

V

A

G

G

V

L

I

L

F

V

K

L

A

N

D

Y

I

S

T

N

D

D

P

Y

S

N

N

H

Y

W

R

L

A

A

A

T

K

K

H

S

L

L

D

G

A

Q

W

W

L

L

K

Q

A

E

R

E

G

K

V

V

I

P

G

A

L

I

C

Y

G

G

I

V

D

D

T

T

R

R

A

M

L

L

T

T

A

K

W

I

I

I

R

R

E

D

N

K

G

G

M

T

P

M

N

L

A

G

V

K

I

I

A

E

H

F

D

E

P

G

S

Q

G

S

V

V

F

D

D

F

I

V

Q

D

T

P

L

N

K

K

A

Q

E

N

A

L

K

-

A

-

W

-

S

-

G

-

L

-

V

-

G

-

L

-

D

-

L

I

A

A

K

E

V

V

A

S

T

T

S

K

G

D

Q

V

S

K

Y

-

R

-

W

-

N

-

E

-

K

-

P

-

W

-

A

V

W

F

N

G

E

K

G

G

H

N

A

P

T

T

L

-

G

-

E

-

T

-

E

-

A

-

Y

-

H

K

V

V

A

A

L

V

D

D

Y

C

G

G

V

I

K

K

R

N

N

N

I

V

L

I

R

R

L

L

F

L

A

V

G

K

L

R

D

G

C

A

R

E

V

V

T

H

V

L

V

V

P

P

A

W

A

N

T

-

S

-

A

-

D

H

E

D

V

F

L

T

A

Q

L

M

K

E

P

Y

D

D

G

G

I

L

F

L

L

I

S

A

N

G

G

G

P

P

G

G

D

N

P

P

A

A

A

-

T

-

G

-

E

-

Y

L

A

A

V

Q

P

P

V

L

I

I

K

Q

N

N

-

V

-

K

L

I

L

L

K

E

S

S

D

D

I

R

-
x
K

-

E

P

P

V

L

F

F

G

G

I

I

C

S

L

T

G

G

H

N

Q

I

M

I

L

T

A

G

L

L

A

A

L

A

G

G

A

A

K

K

T

S

E

Y

K

K

M

M

H

S

Q

M

G

A

H

N

H

R

G

G

A

Q

N

N

H

Q

P

P

V

V

K

L

D

N

H

I

T

T

T

N

G

R

K

Q

V

A

E

F

I

I

V

T

S

A

M

Q

N

N

H

H

G

G

F

Y

A

A

V

L

D

D

S

N

K

-

S

T

L

L

P

P

E

A

G

G

V

W

E

K

E

P

T

L

H

F

I

V

S

N

L

V

F

N

D

D

G

Q

T

T

N

N

C

E

G

G

L

I

R

M

V

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N

E

G

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K

P

P

V

F

F

F

S

A

V

V

Q

Q

H

F

H

H

P

P

E

E

A

V

S

S

P

P

G

G

P

P

Q

T

D

D

S

T

H

E

Y

Y

L

L

F

F

R

D

R

S

F

F

L

F

N

S

L

L

I

I

R

K

E

K

K

G

K

K

G

G

K44 (≠ P12) modified: N6-acetyllysine; alternate; modified: N6-succinyllysine; alternate
K287 (vs. gap) modified: N6-acetyllysine; alternate; modified: N6-succinyllysine; alternate

Sites not aligning to the query:

1291 modified: N6-acetyllysine; alternate; modified: N6-succinyllysine; alternate

P07756 Carbamoyl-phosphate synthase [ammonia], mitochondrial; Carbamoyl-phosphate synthetase I; CPSase I; EC 6.3.4.16 from Rattus norvegicus (Rat) (see 2 papers)
35% identity, 97% coverage: 8:394/401 of query aligns to 40:403/1500 of P07756

query
sites
P07756

T

S

T

V

Q

K

K

A

P

Q

T

T

A

A

L

H

L

I

V

V

L

L

A

E

D

D

G

G

T

T

V

K

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M

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K

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G

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G

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A

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T

P

G

S

K

S

V

V

Q

A

A

G

E

E

V

V

C

V

F

F

N

N

T

T

A

G

L

L

T

G

G

G

Y

Y

Q

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E

E

I

A

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L

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T

D

D

P

P

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A

Y

Y

L

K

G

G

Q

Q

I

I

V

L

T

T

F

M

T

A

F

N

P

P

H

I

I

I

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G

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N

I

G

G

G

A

A

N

P

D

D

E

T

D

T

I

A

E

R

D

D

L

E

T

L

P

G

A

L

A

N

R

K

H

Y

-

M

-

E

-

S

-

D

-

G

-

I

G

K

A

V

V

A

G

G

V

L

I

L

F

V

K

L

A

N

D

Y

I

S

T

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D

D

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Y

S

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N

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Y

W

R

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A

A

A

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W

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A

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A

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G

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V

F

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D

T

P

L

N

K

K

A

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N

A

L

K

-

A

-

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-

S

-

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-

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-

G

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L

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A

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K

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V

A

S

T

T

S

K

G

D

Q

V

S

K

Y

-

R

-

W

-

N

-

E

-

K

-

P

-

W

-

A

V

W

F

N

G

E

K

G

G

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N

A

P

T

T

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-

G

-

E

-

T

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E

-

A

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Y

-

H

K

V

V

A

A

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D

Y

C

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I

K

K

R

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N

N

I

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K

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G

C

A

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P

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T

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S

-

A

-

D

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V

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A

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M

K

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Y

D

D

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G

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I

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G

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G

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D

N

P

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A

A

A

-

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-

G

-

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-

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A

A

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Q

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P

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I

I

K

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N

N

-

V

-

K

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K

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S

D

D

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R

-

K

-

E

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P

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F

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G

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I

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G

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I

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A

G

L

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A

A

L

A

G

G

A

A

K

K

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Y

K

K

M

M

H

S

Q

M

G

A

H

N

H

R

G

G

A

Q

N

N

H

Q

P

P

V

V

K

L

D

N

H

I

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T

T

N

G

R

K

Q

V

A

E

F

I

I

V

T

S

A

M

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N

N

H

H

G

G

F

Y

A

A

V

L

D

D

S

N

K

-

S

T

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L

P

P

E

A

G

G

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W

E

K

E

P

T

L

H

F

I

V

S

N

L

V

F

N

D

D

G

Q

T

T

N

N

C

E

G

G

L

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M

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H

N

E

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S

K

K

P

P

V

F

F

F

S

A

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V

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Q

H

F

H

H

P

P

E

E

A

V

S

S

P

P

G

G

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P

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T

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D

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T

H

E

Y

Y

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F

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R

S

F

F

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F

N

S

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L

I

I

R

K

E

K

K

G

K

K

G

G

Sites not aligning to the query:

537 modified: carbohydrate, O-linked (GlcNAc) serine; alternate
1331 modified: carbohydrate, O-linked (GlcNAc) serine
1332 modified: carbohydrate, O-linked (GlcNAc) threonine
1391 T→V: 400-fold increase in the activation constant of NAG. 3-fold decrease in the reaction rate at saturation of NAG.
1394 T→A: 900-fold increase in the activation constant of NAG. 3-fold decrease in the reaction rate at saturation of NAG.
1410 W→K: 60-fold increase in the activation constant of NAG.
1437 N→D: 70-fold increase in the activation constant of NAG.
1440 N→D: 110-fold increase in the activation constant of NAG. Modifies the specificity for the activator: Binds Phe-NAG considerably better than NAG.

P31327 Carbamoyl-phosphate synthase [ammonia], mitochondrial; Carbamoyl-phosphate synthetase I; CPSase I; EC 6.3.4.16 from Homo sapiens (Human) (see 22 papers)
34% identity, 98% coverage: 1:394/401 of query aligns to 23:403/1500 of P31327

query
sites
P31327

M

V

T

T

A

A

T

H

P

Q

A

K

W

W

T

K

T

F

Q

S

K

R

P

P

-

G

-

I

-

R

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S

-

V

-

K

-

A

-

Q

T

T

A

A

L

H

L

I

V

V

L

L

A

E

D

D

G

G

T

T

V
x
K

I

M

E

K

G

G

K

Y

G

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F

G

G

A

H

T

P

G

S

K

S

V

V

Q

A

A

G

E

E

V

V

C

V

F

F

N

N

T

T

A

G

L

L

T

G

G

G

Y

Y

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P

E

E

I

A

L

I

T

T

D

D

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S

A

Y

Y

L

K

G

G

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Q

I

I

V

L

T

T

F

M

T

A

F

N

P

P

H

I

I

I

G

G

N

N

I

G

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G

A

A

N

P

D

D

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I

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E

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D

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E

T

L

P

G

A

L

A

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Y

-

L

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x
S

-

N

-

G

-

I

G

K

A

V

V

S

G

G

V

L

I

L

F

V

K

L

A

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D

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N

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x
K

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|
R

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x
K

G

G

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M

N

L

A

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I

A

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V

F

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F

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N

A

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-

A

-

W

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G

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A

A

K

E

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V

A

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T

T

S
x
K

G

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Q

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x
K

Y

-

R

-

W

-

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-

E

-

K

-

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-

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A

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N

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x
K

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A

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G

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-

T

-

E

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A

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H
x
K

V

V

A

A

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Y

C

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|
K

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N

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x
K

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-

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-

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N

-

V

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x
K

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S

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x
A

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A

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|
K

T

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K
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K

M

M

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S

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M

G

A

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N

H

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G

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M

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|
H

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x
T

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L

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A

G

G

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K

E

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x
N

L

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N

D

D

G

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T

T

N

N

C

E

G

G

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V

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E

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|
Y

L
|
L

F

F

R

D

R

S

F

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S

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L

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I

R

K

E

K

K
x
G

K
|
K

G

A

K55 (≠ V23) modified: N6-glutaryllysine; alternate
S123 (vs. gap) to F: in CPS1D; modestly decreases enzyme activity; to Y: in CPS1D; uncertain significance
K171 (≠ A133) modified: N6-glutaryllysine; alternate
R174 (= R136) to W: in CPS1D; uncertain significance; dbSNP:rs1553509661
K176 (≠ N138) modified: N6-glutaryllysine
K207 (≠ S179) modified: N6-glutaryllysine; alternate
K210 (≠ S182) modified: N6-glutaryllysine; alternate
K214 (≠ E194) modified: N6-glutaryllysine; alternate
K219 (≠ H207) modified: N6-glutaryllysine; alternate
K228 (= K216) modified: N6-glutaryllysine; alternate
K237 (≠ G225) modified: N6-glutaryllysine
K280 (vs. gap) modified: N6-glutaryllysine; alternate
A304 (≠ L294) to V: in CPS1D; associated with T-986; dbSNP:rs775920437
K307 (= K297) modified: N6-glutaryllysine; alternate
K310 (= K300) modified: N6-glutaryllysine; alternate
H337 (= H327) to R: in CPS1D; modestly decreases enzyme activity; dbSNP:rs28940283
T344 (≠ S335) to A: no negative effect on protein stability, enzyme activity and thermal stability; dbSNP:rs1047883
N355 (≠ S346) to D: in CPS1D; around 80% decrease in enzyme activity; significant reduction in thermal stability; approximately 4-fold decrease in the apparent Vmax for ATP, bicarbonate and ammonia; dbSNP:rs1472190012
Y389 (= Y380) to C: in CPS1D; around 40% decrease in enzyme activity; significant loss of thermal stability
L390 (= L381) to R: in CPS1D; significant loss of protein stability
G401 (≠ K392) to R: in CPS1D; uncertain significance; associated with N-937 in a patient; dbSNP:rs760895692
K402 (= K393) modified: N6-glutaryllysine; alternate

Sites not aligning to the query:

412 modified: N6-glutaryllysine; alternate
438 A → P: in CPS1D; almost complete loss of enzyme activity; dbSNP:rs772497399
453 modified: N6-glutaryllysine; alternate
458 modified: N6-glutaryllysine; alternate
527 modified: N6-glutaryllysine; alternate
530 G → V: found in a patient with VACTERL syndrome and postsurgical PHN; uncertain significance; dbSNP:rs1250316045
532 modified: N6-glutaryllysine; alternate
544 T → M: in CPS1D; almost complete loss of enzyme activity; approximately 60-fold increase in the apparent Km for bicarbonate and approximately 4-fold respective decrease and increase in the apparent Vmax and Km for ammonia; dbSNP:rs121912592
553 modified: N6-glutaryllysine; alternate
678 Q → P: in CPS1D; results in a poor enzyme expression and solubility; hampers correct enzyme folding
728 modified: N6-glutaryllysine
757 modified: N6-glutaryllysine; alternate
772 modified: N6-glutaryllysine; alternate
774 P → L: in CPS1D; the enzyme is inactive
793 modified: N6-glutaryllysine; alternate
811 modified: N6-glutaryllysine; alternate
841 modified: N6-glutaryllysine; alternate
843 L → S: in CPS1D; associated in cis with E-875; causes 70% decrease of enzyme activity; significant decrease in protein yield
850 R → C: in CPS1D; moderate decrease in protein yield and partial loss of enzyme activity; dbSNP:rs1015051007; R → H: in CPS1D; partial loss of enzyme activity; dbSNP:rs767694281
856 modified: N6-glutaryllysine; alternate
869 modified: N6-glutaryllysine
871 T → P: in CPS1D; significant decrease in protein yield and enzyme activity
875 modified: N6-glutaryllysine; alternate; K → E: associated in cis with S-843 in a patient with carbamoyl-phosphate synthase deficiency; does not affect enzyme activity; significant decrease in protein yield and thermal stability; dbSNP:rs147062907
889 modified: N6-glutaryllysine; alternate
892 modified: N6-glutaryllysine; alternate
905 modified: N6-glutaryllysine
908 modified: N6-glutaryllysine; alternate
911 G → E: in CPS1D; significant decrease in protein yield and enzyme activity; dbSNP:rs1388955593; G → V: in CPS1D; significant decrease in protein yield and enzyme activity
913 S → L: in CPS1D; significant decrease in protein yield and partial loss of enzyme activity; dbSNP:rs754706559
914 D → G: in CPS1D; significant decrease in protein yield and enzyme activity; D → H: in CPS1D; significant decrease in protein yield and enzyme activity; dbSNP:rs765484849
915 modified: N6-glutaryllysine; alternate
918 S → P: in CPS1D; significant decrease in protein yield and enzyme activity
919 modified: N6-glutaryllysine; alternate
932 R → T: in CPS1D; significant decrease in protein yield and partial loss of enzyme activity
937 I → N: in CPS1D; associated with R-401; significant decrease in protein yield and enzyme activity; dbSNP:rs760714614
949 A → T: in CPS1D; partial loss of enzyme activity and significant decrease in thermal stability; dbSNP:rs537170841
958 L → P: in CPS1D; significant decrease in protein yield and enzyme activity
959 Y → C: in CPS1D; significant decrease in protein yield and thermal stability; partial loss of enzyme activity; dbSNP:rs1191587211
962 Y → C: in CPS1D; significant decrease in protein yield and partial loss of enzyme activity; dbSNP:rs955666400
964 G → D: in CPS1D; significant decrease in protein yield and enzyme activity; dbSNP:rs534815243
986 I → T: in CPS1D; associated with V-304; dbSNP:rs1553516442
987 G → C: in CPS1D; may affect splicing; dbSNP:rs1553516443
1074 modified: N6-glutaryllysine; alternate
1150 modified: N6-glutaryllysine
1168 modified: N6-glutaryllysine; alternate
1183 modified: N6-glutaryllysine; alternate
1215 I → V: in CPS1D; uncertain significance; dbSNP:rs141373204
1224 modified: N6-glutaryllysine
1254 I → F: in CPS1D; uncertain significance
1266 F → S: in dbSNP:rs1047886
1283 M → L: in dbSNP:rs1047887
1356 modified: N6-glutaryllysine; alternate
1360 modified: N6-glutaryllysine; alternate
1363:1366 natural variant: Missing (in CPS1D; uncertain significance)
1376 G → S: no functional consequences; no negative effect on protein stability, enzyme activity and thermal stability; dbSNP:rs140578009
1378 A → T: in CPS1D; significant reduction in thermal stability; dbSNP:rs1245373037
1381 L → S: in CPS1D; significant loss of protein stability
1406 T → N: probable risk factor for PHN; dbSNP:rs1047891
1411 P → L: in CPS1D; modestly decreases enzyme activity; dbSNP:rs1202306773
1443 T → A: in CPS1D; almost complete loss of enzyme activity; approximately 10-fold decrease in the apparent Vmax for bicarbonate, ammonia and ATP; decreased affinity for NAG
1453 R → Q: in CPS1D; the enzyme is inactive; R → W: in CPS1D; the enzyme is inactive; dbSNP:rs933813349
1479 modified: N6-glutaryllysine; alternate
1486 modified: N6-glutaryllysine; alternate
1491 Y → H: in CPS1D; triggers a large decrease in the apparent affinity for N-acetyl-L-glutamate (NAG); dbSNP:rs1553519513

6w2jB Cps1 bound to allosteric inhibitor h3b-374 (see paper)
35% identity, 95% coverage: 13:394/401 of query aligns to 3:361/1364 of 6w2jB

query
sites
6w2jB

T

T

A

A

L

H

L

I

V

V

L

L

A

E

D

D

G

G

T

T

V

K

I

M

E

K

G

G

K

Y

G

S

I

F

G

G

A

H

T

P

G

S

K

S

V

V

Q

A

A

G

E

E

V

V

C

V

F

F

N

N

T

T

A

G

L

L

T

G

G

G

Y

Y

Q

P

E

E

I

A

L

I

T

T

D

D

P

P

S

A

Y

Y

L

K

G

G

Q

Q

I

I

V

L

T

T

F

M

T

A

F

N

P

P

H

I

I

I

G

G

N

N

I

G

G

G

A

A

N

P

D

D

E

T

D

T

I

A

E

L

D

D

L

E

T

L

P

G

A

L

A

S

R

K

H

Y

-

L

-

E

-

S

-

N

-

G

-

I

G

K

A

V

V

S

G

G

V

L

I

L

F

V

K

L

A

D

D

Y

I

S

T

K

D

D

P

Y

S

N

N

H

Y

W

R

L

A

A

A

T

K

K

H

S

L

L

D

G

A

Q

W

W

L

L

K

Q

A

E

R

E

G

K

V

V

I

P

G

A

L

I

C

Y

G

G

I

V

D

D

T

T

R

R

A

M

L

L

T

T

A

K

W

I

I

I

R

R

E

D

N

K

G

G

M

T

P

M

N

L

A

G

V

K

I

I

A

E

H

F

D

E

P

G

S

Q

G

P

V

V

F

D

D

F

I

V

Q

D

T

P

L

N

K

K

A

Q

E

N

A

L

K

-

A

-

W

-

S

-

G

-

L

-

V

-

G

-

L

-

D

-

L

I

A

A

K

E

V

V

A

S

T

T

S

K

G

D

Q

V

S

K

Y

-

R

-

W

-

N

-

E

-

K

-

P

-

W

-

A

V

W

Y

N

G

E

K

G

G

H

N

A

P

T

T

L

-

G

-

E

-

T

-

E

-

A

-

Y

-

H

K

V

V

A

A

L

V

D

D

Y

C

G

G

V

I

K

K

R

N

N

N

I

V

L

I

R

R

L

L

F

L

A

V

G

K

L

R

D

G

C

A

R

E

V

V

T

H

V

L

V

V

P

P

A

W

A

N

T

-

S

-

A

-

D

H

E

D

V

F

L

T

A

K

L

M

K

E

P

Y

D

D

G

G

I

I

F

L

L

I

S

A

N

G

G

G

P

P

G

G

D

N

P

P

A

A

A

-

T

-

G

-

E

-

Y

L

A

A

V

E

P

P

V

L

I

I

K

Q

N

N

-

V

-

R

-

K

L

I

L

L

K

E

S

S

D

D

I

R

-

K

-

E

P

P

V

L

F

F

G

G

I

I

C

S

L

T

G

G

H

N

Q

L

M

I

L

T

A

G

L

L

A

A

L

A

G

G

A

A

K

K

T

T

E

Y

K

K

M

M

H

S

Q

M

G

A

H
x
N

H

R

G

G

A

Q

N

N

H

Q

P

P

V

V

K

L

D

N

H

I

T

T

T

N

G

K

K

Q

V

A

E

F

I

I

V

T

S

A

M
x
Q

N

N

H
|
H

G

G

F

Y

A

A

V

L

D

D

S

N

K

-

S

T

L

L

P

P

E

A

G

G

V

W

E

K

E

P

T

L

H

F

I

V

S

N

L

V

F

N

D

D

G

Q

T

T

N

N

C
x
E

G

G

L

I

R

M

V

H

N

E

G

S

K

K

P

P

V

F

F

F

S

A

V

V

Q

Q

H

F

H

H

P

P

E

E

A

V

S

T

P

P

G

G

P

P

Q

I

D

D

S

T

H

E

Y

Y

L

L

F

F

R

D

R

S

F

F

L

F

N

S

L

L

I

I

R

K

E

K

K

G

K

K

G

A

binding zinc ion: N273 (≠ H305), Q293 (≠ M325), H295 (= H327), E320 (≠ C353)

Sites not aligning to the query:

active site: 593, 607, 609, 615, 811, 937, 1055, 1191
binding (2-fluoranyl-4-methoxy-phenyl)-[(3~{R},5~{R})-4-(2-fluoranyl-4-methoxy-phenyl)carbonyl-3,5-dimethyl-piperazin-1-yl]methanone: 547, 549, 557, 668, 669, 672, 673, 698, 702, 705, 706, 737, 739, 740

6w2jA Cps1 bound to allosteric inhibitor h3b-374 (see paper)
35% identity, 95% coverage: 13:394/401 of query aligns to 3:361/1422 of 6w2jA

query
sites
6w2jA

T

T

A

A

L

H

L

I

V

V

L

L

A

E

D

D

G

G

T

T

V

K

I

M

E

K

G

G

K

Y

G

S

I

F

G

G

A

H

T

P

G

S

K

S

V

V

Q

A

A

G

E

E

V

V

C

V

F

F

N

N

T

T

A

G

L

L

T

G

G

G

Y

Y

Q

P

E

E

I

A

L

I

T

T

D

D

P

P

S

A

Y

Y

L

K

G

G

Q

Q

I

I

V

L

T

T

F

M

T

A

F

N

P

P

H

I

I

I

G

G

N

N

I

G

G

G

A

A

N

P

D

D

E

T

D

T

I

A

E

L

D

D

L

E

T

L

P

G

A

L

A

S

R

K

H

Y

-

L

-

E

-

S

-

N

-

G

-

I

G

K

A

V

V

S

G

G

V

L

I

L

F

V

K

L

A

D

D

Y

I

S

T

K

D

D

P

Y

S

N

N

H

Y

W

R

L

A

A

A

T

K

K

H

S

L

L

D

G

A

Q

W

W

L

L

K

Q

A

E

R

E

G

K

V

V

I

P

G

A

L

I

C

Y

G

G

I

V

D

D

T

T

R

R

A

M

L

L

T

T

A

K

W

I

I

I

R

R

E

D

N

K

G

G

M

T

P

M

N

L

A

G

V

K

I

I

A

E

H

F

D

E

P

G

S

Q

G

P

V

V

F

D

D

F

I

V

Q

D

T

P

L

N

K

K

A

Q

E

N

A

L

K

-

A

-

W

-

S

-

G

-

L

-

V

-

G

-

L

-

D

-

L

I

A

A

K

E

V

V

A

S

T

T

S

K

G

D

Q

V

S

K

Y

-

R

-

W

-

N

-

E

-

K

-

P

-

W

-

A

V

W

Y

N

G

E

K

G

G

H

N

A

P

T

T

L

-

G

-

E

-

T

-

E

-

A

-

Y

-

H

K

V

V

A

A

L

V

D

D

Y

C

G

G

V

I

K

K

R

N

N

N

I

V

L

I

R

R

L

L

F

L

A

V

G

K

L

R

D

G

C

A

R

E

V

V

T

H

V

L

V

V

P

P

A

W

A

N

T

-

S

-

A

-

D

H

E

D

V

F

L

T

A

K

L

M

K

E

P

Y

D

D

G

G

I

I

F

L

L

I

S

A

N

G

G

G

P

P

G

G

D

N

P

P

A

A

A

-

T

-

G

-

E

-

Y

L

A

A

V

E

P

P

V

L

I

I

K

Q

N

N

-

V

-

R

-

K

L

I

L

L

K

E

S

S

D

D

I

R

-

K

-

E

P

P

V

L

F

F

G

G

I

I

C

S

L

T

G

G

H

N

Q

L

M

I

L

T

A

G

L

L

A

A

L

A

G

G

A

A

K

K

T

T

E

Y

K

K

M

M

H

S

Q

M

G

A

H
x
N

H

R

G

G

A

Q

N

N

H

Q

P

P

V

V

K

L

D

N

H

I

T

T

T

N

G

K

K

Q

V

A

E

F

I

I

V

T

S

A

M
x
Q

N

N

H
|
H

G

G

F

Y

A

A

V

L

D

D

S

N

K

-

S

T

L

L

P

P

E

A

G

G

V

W

E

K

E

P

T

L

H

F

I

V

S

N

L

V

F

N

D

D

G

Q

T

T

N

N

C
x
E

G

G

L

I

R

M

V

H

N

E

G

S

K

K

P

P

V

F

F

F

S

A

V

V

Q

Q

H

F

H

H

P

P

E

E

A

V

S

T

P

P

G

G

P

P

Q

I

D

D

S

T

H

E

Y

Y

L

L

F

F

R

D

R

S

F

F

L

F

N

S

L

L

I

I

R

K

E

K

K

G

K

K

G

A

binding zinc ion: N273 (≠ H305), Q293 (≠ M325), H295 (= H327), E320 (≠ C353)

Sites not aligning to the query:

active site: 651, 665, 667, 673, 869, 995, 1113, 1249
binding (2-fluoranyl-4-methoxy-phenyl)-[(3~{R},5~{R})-4-(2-fluoranyl-4-methoxy-phenyl)carbonyl-3,5-dimethyl-piperazin-1-yl]methanone: 605, 607, 615, 725, 726, 727, 730, 732, 756, 760, 763, 764, 795, 797, 798

6uelA Cps1 bound to allosteric inhibitor h3b-193 (see paper)
35% identity, 95% coverage: 13:394/401 of query aligns to 3:361/1333 of 6uelA

query
sites
6uelA

T

T

A

A

L

H

L

I

V

V

L

L

A

E

D

D

G

G

T

T

V

K

I

M

E

K

G

G

K

Y

G

S

I

F

G

G

A

H

T

P

G

S

K

S

V

V

Q

A

A

G

E

E

V

V

C

V

F

F

N

N

T

T

A

G

L

L

T

G

G

G

Y

Y

Q

P

E

E

I

A

L

I

T

T

D

D

P

P

S

A

Y

Y

L

K

G

G

Q

Q

I

I

V

L

T

T

F

M

T

A

F

N

P

P

H

I

I

I

G

G

N

N

I

G

G

G

A

A

N

P

D

D

E

T

D

T

I

A

E

L

D

D

L

E

T

L

P

G

A

L

A

S

R

K

H

Y

-

L

-

E

-

S

-

N

-

G

-

I

G

K

A

V

V

S

G

G

V

L

I

L

F

V

K

L

A

D

D

Y

I

S

T

K

D

D

P

Y

S

N

N

H

Y

W

R

L

A

A

A

T

K

K

H

S

L

L

D

G

A

Q

W

W

L

L

K

Q

A

E

R

E

G

K

V

V

I

P

G

A

L

I

C

Y

G

G

I

V

D

D

T

T

R

R

A

M

L

L

T

T

A

K

W

I

I

I

R

R

E

D

N

K

G

G

M

T

P

M

N

L

A

G

V

K

I

I

A

E

H

F

D

E

P

G

S

Q

G

P

V

V

F

D

D

F

I

V

Q

D

T

P

L

N

K

K

A

Q

E

N

A

L

K

-

A

-

W

-

S

-

G

-

L

-

V

-

G

-

L

-

D

-

L

I

A

A

K

E

V

V

A

S

T

T

S

K

G

D

Q

V

S

K

Y

-

R

-

W

-

N

-

E

-

K

-

P

-

W

-

A

V

W

Y

N

G

E

K

G

G

H

N

A

P

T

T

L

-

G

-

E

-

T

-

E

-

A

-

Y

-

H

K

V

V

A

A

L

V

D

D

Y

C

G

G

V

I

K

K

R

N

N

N

I

V

L

I

R

R

L

L

F

L

A

V

G

K

L

R

D

G

C

A

R

E

V

V

T

H

V

L

V

V

P

P

A

W

A

N

T

-

S

-

A

-

D

H

E

D

V

F

L

T

A

K

L

M

K

E

P

Y

D

D

G

G

I

I

F

L

L

I

S

A

N

G

G

G

P

P

G

G

D

N

P

P

A

A

A

-

T

-

G

-

E

-

Y

L

A

A

V

E

P

P

V

L

I

I

K

Q

N

N

-

V

-

R

-

K

L

I

L

L

K

E

S

S

D

D

I

R

-

K

-

E

P

P

V

L

F

F

G

G

I

I

C

S

L

T

G

G

H

N

Q

L

M

I

L

T

A

G

L

L

A

A

L

A

G

G

A

A

K

K

T

T

E

Y

K

K

M

M

H

S

Q

M

G

A

H
x
N

H

R

G

G

A

Q

N

N

H

Q

P

P

V

V

K

L

D

N

H

I

T

T

T

N

G

K

K

Q

V

A

E

F

I

I

V

T

S

A

M
x
Q

N

N

H
|
H

G

G

F

Y

A

A

V

L

D

D

S

N

K

-

S

T

L

L

P

P

E

A

G

G

V

W

E

K

E

P

T

L

H

F

I

V

S

N

L

V

F

N

D

D

G

Q

T

T

N

N

C
x
E

G

G

L

I

R

M

V

H

N

E

G

S

K

K

P

P

V

F

F

F

S

A

V

V

Q

Q

H

F

H

H

P

P

E

E

A

V

S

T

P

P

G

G

P

P

Q

I

D

D

S

T

H

E

Y

Y

L

L

F

F

R

D

R

S

F

F

L

F

N

S

L

L

I

I

R

K

E

K

K

G

K

K

G

A

binding zinc ion: N273 (≠ H305), Q293 (≠ M325), H295 (= H327), E320 (≠ C353)

Sites not aligning to the query:

active site: 587, 601, 603, 609, 803, 929, 1032, 1168
binding N~1~-[(4-fluorophenyl)methyl]-N~1~-methyl-N~4~-(4-methyl-1,3-thiazol-2-yl)piperidine-1,4-dicarboxamide: 544, 551, 662, 663, 665, 690, 698, 701, 729, 731, 732

5douD Crystal structure of human carbamoyl phosphate synthetase i (cps1), ligand-bound form (see paper)
35% identity, 95% coverage: 13:394/401 of query aligns to 3:361/1430 of 5douD

query
sites
5douD

T

T

A

A

L

H

L

I

V

V

L

L

A

E

D

D

G

G

T

T

V

K

I

M

E

K

G

G

K

Y

G

S

I

F

G

G

A

H

T

P

G

S

K

S

V

V

Q

A

A

G

E

E

V

V

C

V

F

F

N

N

T

T

A

G

L

L

T

G

G

G

Y

Y

Q

P

E

E

I

A

L

I

T

T

D

D

P

P

S

A

Y

Y

L

K

G

G

Q

Q

I

I

V

L

T

T

F

M

T

A

F

N

P

P

H

I

I

I

G

G

N

N

I

G

G

G

A

A

N

P

D

D

E

T

D

T

I

A

E

L

D

D

L

E

T

L

P

G

A

L

A

S

R

K

H

Y

-

L

-

E

-

S

-

N

-

G

-

I

G

K

A

V

V

S

G

G

V

L

I

L

F

V

K

L

A

D

D

Y

I

S

T

K

D

D

P

Y

S

N

N

H

Y

W

R

L

A

A

A

T

K

K

H

S

L

L

D

G

A

Q

W

W

L

L

K

Q

A

E

R

E

G

K

V

V

I

P

G

A

L

I

C

Y

G

G

I

V

D

D

T

T

R

R

A

M

L

L

T

T

A

K

W

I

I

I

R

R

E

D

N

K

G

G

M

T

P

M

N

L

A

G

V

K

I

I

A

E

H

F

D

E

P

G

S

Q

G

P

V

V

F

D

D

F

I

V

Q

D

T

P

L

N

K

K

A

Q

E

N

A

L

K

-

A

-

W

-

S

-

G

-

L

-

V

-

G

-

L

-

D

-

L

I

A

A

K

E

V

V

A

S

T

T

S

K

G

D

Q

V

S

K

Y

-

R

-

W

-

N

-

E

-

K

-

P

-

W

-

A

V

W

Y

N

G

E

K

G

G

H

N

A

P

T

T

L

-

G

-

E

-

T

-

E

-

A

-

Y

-

H

K

V

V

A

A

L

V

D

D

Y

C

G

G

V

I

K

K

R

N

N

N

I

V

L

I

R

R

L

L

F

L

A

V

G

K

L

R

D

G

C

A

R

E

V

V

T

H

V

L

V

V

P

P

A

W

A

N

T

-

S

-

A

-

D

H

E

D

V

F

L

T

A

K

L

M

K

E

P

Y

D

D

G

G

I

I

F

L

L

I

S

A

N

G

G

G

P

P

G

G

D

N

P

P

A

A

A

-

T

-

G

-

E

-

Y

L

A

A

V

E

P

P

V

L

I

I

K

Q

N

N

-

V

-

R

-

K

L

I

L

L

K

E

S

S

D

D

I

R

-

K

-

E

P

P

V

L

F

F

G

G

I

I

C
x
S

L

T

G

G

H

N

Q

L

M

I

L

T

A

G

L

L

A

A

L

A

G

G

A

A

K

K

T

T

E

Y

K

K

M

M

H

S

Q

M

G

A

H

N

H

R

G

G

A

Q

N

N

H

Q

P

P

V

V

K

L

D

N

H

I

T

T

T

N

G

K

K

Q

V

A

E

F

I

I

V

T

S

A

M

Q

N

N

H

H

G

G

F

Y

A

A

V

L

D

D

S

N

K

-

S

T

L

L

P

P

E

A

G

G

V

W

E

K

E

P

T

L

H

F

I

V

S

N

L

V

F

N

D

D

G

Q

T

T

N

N

C

E

G

G

L

I

R

M

V

H

N

E

G

S

K

K

P

P

V

F

F

F

S

A

V

V

Q

Q

H

F

H
|
H

P

P

E
|
E

A

V

S

T

P

P

G

G

P

P

Q

I

D

D

S

T

H

E

Y

Y

L

L

F

F

R

D

R

S

F

F

L

F

N

S

L

L

I

I

R

K

E

K

K

G

K

K

G

A

active site: S252 (≠ C284), H335 (= H368), E337 (= E370)

Sites not aligning to the query:

active site: 505, 545, 576, 589, 617, 656, 658, 672, 674, 676, 680, 880, 1006, 1087, 1116, 1124, 1184, 1196, 1198, 1203, 1260
binding adenosine-5'-diphosphate: 505, 543, 545, 550, 551, 552, 581, 583, 584, 585, 589, 614, 615, 616, 617, 658, 671, 672, 1085, 1110, 1111, 1116, 1140, 1142, 1143, 1144, 1184, 1186, 1195, 1196
binding magnesium ion: 658, 672, 672, 674
binding n-acetyl-l-glutamate: 1307, 1308, 1332, 1334, 1335, 1351, 1379, 1384, 1385, 1386, 1390
binding phosphate ion: 550, 551, 617, 672, 674, 676, 679

P00903 Aminodeoxychorismate synthase component 2; ADC synthase; ADCS; 4-amino-4-deoxychorismate synthase component 2; Aminodeoxychorismate synthase, glutamine amidotransferase component; EC 2.6.1.85 from Escherichia coli (strain K12) (see paper)
31% identity, 42% coverage: 218:387/401 of query aligns to 14:186/187 of P00903

query
sites
P00903

N

N

I

L

L

Y

R

Q

L

Y

F

F

A

C

-

E

-

L

G

G

L

A

D

D

C

V

R

L

V

V

T

K

V

R

V

N

P

D

A

A

A

L

T

T

S

L

A

A

D

D

E

-

V

I

L

D

A

A

L

L

K

K

P

P

D

Q

G

K

I

I

F

V

L

I

S

S

N

P

G

G

P

P

G

C

D

T

P

P

A

D

A

E

T

A

G

G

E

-

Y

I

A

S

V

L

P

D

V

V

I

I

K

R

N

H

L

Y

L

-

K

A

S

G

D

R

I

L

P

P

V

I

F

L

G

G

I

V

C
|
C

L

L

G

G

H

H

Q

Q

M

A

L

M

A

A

L

Q

A

A

L

F

G

G

A

G

K

K

T

V

E

V

K

R

M

A

H

A

Q

K

G

V

H

M

H

H

G

G

A

K

N

T

H

S

P

P

V

I

K

T

D

H

H

N

T

G

T

E

G

G

K

V

V

F

E

R

-

G

-

L

-

A

-

N

-

P

-

L

I

T

V

V

S

T

M

R

N

Y

H

H

G

S

F

L

A

V

V

V

D

E

S

P

K

D

S

S

L

L

P

P

E

A

G

C

V

F

E

D

E

V

T

T

H

A

I

W

S

S

L

E

F

T

D

R

G

E

T

I

N

-

C

M

G

G

L

I

R

R

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H

N

R

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K

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P

D

V

L

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Q

H

F

H
|
H

P

P

E
|
E

A

S

S

I

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L

G

S

P

E

Q

Q

D

-

S

G

H

H

Y

Q

L

L

F

L

R

A

R

N

F

F

L

L

N

H

C79 (= C284) mutation to S: 10000-fold decrease in catalytic efficiency.
H168 (= H368) mutation to Q: Loss of activity.
E170 (= E370) mutation to A: 150-fold decrease in catalytic efficiency.; mutation to D: 4-fold decrease in catalytic efficiency.; mutation E->K,Q: Loss of activity.

Query Sequence

>WP_012708791.1 NCBI__GCF_000018545.1:WP_012708791.1
MTATPAWTTQKPTALLVLADGTVIEGKGIGATGKVQAEVCFNTALTGYQEILTDPSYLGQ
IVTFTFPHIGNIGANDEDIEDLTPAARHGAVGVIFKADITDPSNYRAAKHLDAWLKARGV
IGLCGIDTRALTAWIRENGMPNAVIAHDPSGVFDIQTLKAEAKAWSGLVGLDLAKVATSG
QSYRWNEKPWAWNEGHATLGETEAAYHVVALDYGVKRNILRLFAGLDCRVTVVPAATSAD
EVLALKPDGIFLSNGPGDPAATGEYAVPVIKNLLKSDIPVFGICLGHQMLALALGAKTEK
MHQGHHGANHPVKDHTTGKVEIVSMNHGFAVDSKSLPEGVEETHISLFDGTNCGLRVNGK
PVFSVQHHPEASPGPQDSHYLFRRFLNLIREKKGEPALAER

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory