SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012709136.1 NCBI__GCF_000018545.1:WP_012709136.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q56YA5 Serine--glyoxylate aminotransferase; Alanine--glyoxylate aminotransferase; AGT; Asparagine aminotransferase; Serine--pyruvate aminotransferase; EC 2.6.1.45; EC 2.6.1.44; EC 2.6.1.-; EC 2.6.1.51 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
55% identity, 96% coverage: 3:380/395 of query aligns to 8:386/401 of Q56YA5

query
sites
Q56YA5

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TGT 68:70 (≠ SGT 63:65) binding pyridoxal 5'-phosphate
T148 (= T143) binding pyridoxal 5'-phosphate
QK 200:201 (= QK 195:196) binding pyridoxal 5'-phosphate
K201 (= K196) binding 3-hydroxypyruvate
P251 (= P245) mutation to L: Abolishes aminotransferase activity.
R347 (= R341) binding 3-hydroxypyruvate

6pk1A Alanine-glyoxylate aminotransferase 1 (agt1) from arabidopsis thaliana in presence of serine (see paper)
55% identity, 96% coverage: 3:380/395 of query aligns to 6:384/399 of 6pk1A

query
sites
6pk1A

G

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binding 3-hydroxypyruvic acid: F93 (= F90), R345 (= R341)
binding pyridoxal-5'-phosphate: T66 (≠ S63), G67 (= G64), T68 (= T65), W71 (= W68), F93 (= F90), T146 (= T143), D173 (= D170), V175 (= V172), K199 (= K196)

6pk3B Alanine-glyoxylate aminotransferase 1 (agt1) from arabidopsis thaliana (see paper)
55% identity, 96% coverage: 3:380/395 of query aligns to 7:385/400 of 6pk3B

query
sites
6pk3B

G

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binding pyridoxal-5'-phosphate: T67 (≠ S63), G68 (= G64), T69 (= T65), W72 (= W68), F94 (= F90), T147 (= T143), D174 (= D170), V176 (= V172), Q199 (= Q195), K200 (= K196)

3islA Crystal structure of ureidoglycine-glyoxylate aminotransferase (pucg) from bacillus subtilis
30% identity, 95% coverage: 7:381/395 of query aligns to 10:382/387 of 3islA

query
sites
3islA

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T

T

G

V

V

F

-

D

P

P

V

E

D

D

L

I

Y

I

T

R

E

E

R

-

L

-

A

-

D

I

K

K

A

K

H

V

R

K

I

P

K

K

A

I

V

V

F

A

V

M

T

V

H

H

N

G

E

E

T
|
T

A

S

T

T

G

G

V

R

T

I

S

H

D

P

V

L

A

K

A

A

V

I

R

G

A

E

A

A

L

-

D

-

A

C

S

R

G

T

H

E

P

D

A

A

L

L

L

F

F

I

V

V

D
|
D

G

A

V
|
V

S

A

S

T

I

I

G

G

S

G

I

C

D

E

F

V

R

K

Q

V

D

D

E

E

W

W

G

K

V

I

D

D

C

A

A

A

V

I

S

G

G

G

S

T

Q
|
Q

K
|
K

G

C

F

L

M

S

L

V

P

P

A

S

G

G

L

M

G

A

F

P

L

I

S

T

V

Y

S

N

Q

E

K

R

A

V

L

A

E

D

-

V

-

I

A

A

K

R

T

N

A

R

R

P

H

I

M

T

R

S

C

N

Y

Y

F

F

-

D

-

L

-

S

S

Q

F

L

E

E

D

D

M

Y

I

W

K

S

T

E

N

R

D

R

T

L

G

N

Y

H

F

-

P

-

Y

H

T

T

P

E

P

A

T

T

Q

T

L

M

L

L

H

Y

G

A

L

L

R

R

A

E

A

G

L

V

D

R

V

L

I

V

F

L

E

E

G

G

L

L

E

E

N

T

I

R

F

F

A

E

R

R

H

H

R

R

H

H

L

H

A

E

D

A

G

A

V

L

R

A

A

A

A

G

V

I

S

K

A

A

W

M

G

G

L

L

K

R

L

L

C

F

A

G

T

D

E

D

P

-

K

S

W

C

Y

K

S

M

D

P

T

V

V

V

S

T

A

C

I

V

R

E

L

I

P

P

E

G

G

G

I

I

D

D

G

G

V

E

K

S

V

V

I

R

R

D

H

M

A

L

F

L

D

A

T

Q

Y

F

N

G

T

I

S

E

L

I

G

A

S

S

G

S

L

F

S

G

K

P

V

L

A

A

G

G

K

K

V

I

F

W

R

R

I

I

G

G

H

T

L

M

G

G

-

Y

S

S

L

C

N

R

E

K

V

E

M

N

V

V

L

L

G

F

A

V

L

L

S

A

A

G

A

L

E

E

L

A

T

V

L

L

D

R

C

H

G

N

V

A

K

G

I

I

E

E

P

A

G

G

A

K

G

A

V

L

G

Q

A

A

I

L

binding pyridoxal-5'-phosphate: T66 (= T65), S67 (vs. gap), R68 (vs. gap), F93 (= F90), T143 (= T143), D168 (= D170), V170 (= V172), Q193 (= Q195), K194 (= K196)

2huuA Crystal structure of aedes aegypti alanine glyoxylate aminotransferase in complex with alanine (see paper)
32% identity, 86% coverage: 7:347/395 of query aligns to 22:362/385 of 2huuA

query
sites
2huuA

L

L

F

M

I

G

P
|
P

G

G

P

P

T

S

N

N

I

A

P

P

E

Q

Q

R

I

V

R

L

Q

D

A

A

M

M

N

S

L

R

P

P

M

I

E

L

D

G

M
x
H

R

L

S

H

P

P

R

E

Y

-

P

-

E

-

L

-

T

T

L

L

P

K

L

I

F

M

A

D

D

D

V

I

K

K

K

E

-

G

-

V

-

R

-

Y

V

L

F

F

K

Q

N

T

R

N

N

N

G

I

R

A

V

T

F

F

I

C

Y

L

P

S

S

A

S

S

G

G

T

H

G

G

A

G

W

M

E

E

A

A

A

T

M

L

T

C

N

N

V

L

L

L

S

E

P

D

G

G

D

D

R

V

V

I

L

L

M

I

S

G

R

H

F

T

G

G

Q

H

F

W

S

G

H

D

L

R

W

S

V

A

D

D

M

M

A

A

E

T

R

R

L

Y

G

G

L

A

E

D

V

V

D

R

C

V

L

V

D

K

R

S

E

K

W

V

G

G

T

Q

G

S

V

L

P

S

V

L

D

D

L

E

Y

I

T

R

E

D

R

A

L

L

A

L

A

-

D

-

K

-

A

I

H

H

R

K

I

P

K

S

A

V

V

L

F

F

V

L

T

T

H

Q

N

G

E

D

T
x
S

A

S

T

T

G

G

V

V

T

L

S

Q

D

G

V

L

A

E

A

G

V

V

R

G

A

A

A

L

L

-

D

-

A

C

S

H

G

Q

H

H

P

N

A

C

L

L

F

I

V

V

D

D

G

T

V

V

S

A

S

S

I

L

G

G

S

G

I

A

D

P

F

M

R

F

Q

M

D

D

E

R

W

W

G

E

V

I

D

D

C

A

A

M

V

Y

S

T

G

G

S

S

Q

Q

K
|
K

G

V

F

L

M

G

L

A

P

P

A

P

G

G

L

I

G

T

F

P

L

V

S

S

V

F

S

S

Q

H

K

R

A

A

L

V

E

E

A

R

A

Y

K

K

T

R

A

-

R

R

H

N

M

T

R

K

C

V

Y

K

F

V

S

Y

F

Y

E

W

D

D

M

M

I

S

K

L

T

V

N

G

D

D

-

Y

-

W

-

G

-

C

-

F

-

G

-

R

T

P

G

R

Y

I

F
x
Y

P

H

Y

H

T

T

P

I

P

S

T

S

Q

T

L

L

H

Y

G

G

L

L

R

R

A

E

A

A

L

I

D

A

V

M

I

A

F

C

E

E

G

G

L

L

E

P

N

A

I

L

F

I

A

A

R

R

H

H

R

E

H

D

L

C

A

A

D

K

G

R

V

L

R

Y

A

R

A

G

V

L

S

Q

A

D

W

A

G

G

L

F

K

E

L

L

C

Y

A

A

T

-

E

D

P

P

K

K

W

D

Y

R

S

L

D

S

T

T

V

V

S

T

A

T

I

I

R

K

L

V

P

P

E

Q

G

G

I

V

D

D

G

W

V

L

K

K

V

A

I

A

R

Q

H

Y

A

A

F

M

D

K

T

T

Y

Y

N

L

T

V

S

E

L

I

G

S

S

G

G

G

L
|
L

S

G

K

P

V

T

A

A

G

G

K

Q

V

V

F

F

R
|
R

I

I

G

G

H

L

L

M

G

G

S

Q

binding 1-butanol: H45 (≠ M30), Y257 (≠ F241)
binding alanine: P25 (= P10), S155 (≠ T143), K206 (= K196), L347 (= L332), R356 (= R341)

2huiA Crystal structure of aedes aegypti alanine glyoxylate aminotransferase in complex with glyoxylic acid (see paper)
32% identity, 86% coverage: 7:347/395 of query aligns to 22:362/385 of 2huiA

query
sites
2huiA

L

L

F

M

I

G

P
|
P

G

G

P

P

T

S

N

N

I

A

P

P

E

Q

Q

R

I

V

R

L

Q

D

A

A

M

M

N

S

L

R

P

P

M

I

E

L

D

G

M

H

R

L

S

H

P

P

R

E

Y

-

P

-

E

-

L

-

T

T

L

L

P

K

L

I

F

M

A

D

D

D

V

I

K

K

K

E

-

G

-

V

-

R

-

Y

V

L

F

F

K

Q

N

T

R

N

N

N

G

I

R

A

V

T

F

F

I

C

Y

L

P

S

S

A

S

S

G

G

T

H

G

G

A

G

W

M

E

E

A

A

A

T

M

L

T

C

N

N

V

L

L

L

S

E

P

D

G

G

D

D

R

V

V

I

L

L

M

I

S

G

R

H

F

T

G

G

Q

H

F

W

S

G

H

D

L

R

W

S

V

A

D

D

M

M

A

A

E

T

R

R

L

Y

G

G

L

A

E

D

V

V

D

R

C

V

L

V

D

K

R

S

E

K

W

V

G

G

T

Q

G

S

V

L

P

S

V

L

D

D

L

E

Y

I

T

R

E

D

R

A

L

L

A

L

A

-

D

-

K

-

A

I

H

H

R

K

I

P

K

S

A

V

V

L

F

F

V

L

T

T

H

Q

N

G

E

D

T
x
S

A

S

T

T

G

G

V

V

T

L

S

Q

D

G

V

L

A

E

A

G

V

V

R

G

A

A

A

L

L

-

D

-

A

C

S

H

G

Q

H

H

P

N

A

C

L

L

F

I

V

V

D

D

G

T

V

V

S

A

S

S

I

L

G

G

S

G

I

A

D

P

F

M

R

F

Q

M

D

D

E

R

W

W

G

E

V

I

D

D

C

A

A

M

V

Y

S

T

G

G

S

S

Q

Q

K
|
K

G

V

F

L

M

G

L

A

P

P

A

P

G

G

L

I

G

T

F

P

L

V

S

S

V

F

S

S

Q

H

K

R

A

A

L

V

E

E

A

R

A

Y

K

K

T

R

A

-

R

R

H

N

M

T

R

K

C

V

Y

K

F

V

S

Y

F

Y

E

W

D

D

M

M

I

S

K

L

T

V

N

G

D

D

-

Y

-

W

-

G

-

C

-

F

-

G

-

R

T

P

G

R

Y

I

F

Y

P

H

Y

H

T

T

P

I

P

S

T

S

Q

T

L

L

H

Y

G

G

L

L

R

R

A

E

A

A

L

I

D

A

V

M

I

A

F

C

E

E

G

G

L

L

E

P

N

A

I

L

F

I

A

A

R

R

H

H

R

E

H

D

L

C

A

A

D

K

G

R

V

L

R

Y

A

R

A

G

V

L

S

Q

A

D

W

A

G

G

L

F

K

E

L

L

C

Y

A

A

T

-

E

D

P

P

K

K

W

D

Y

R

S

L

D

S

T

T

V

V

S

T

A

T

I

I

R

K

L

V

P

P

E

Q

G

G

I

V

D

D

G

W

V

L

K

K

V

A

I

A

R

Q

H

Y

A

A

F

M

D

K

T

T

Y

Y

N

L

T

V

S

E

L

I

G

S

S

G

G

G

L

L

S

G

K

P

V

T

A

A

G

G

K

Q

V

V

F

F

R
|
R

I

I

G

G

H

L

L

M

G

G

S

Q

binding glyoxylic acid: P25 (= P10), S155 (≠ T143), K206 (= K196), R356 (= R341)

2hufA Crystal structure of aedes aegypti alanine glyoxylate aminotransferase (see paper)
32% identity, 86% coverage: 7:347/395 of query aligns to 22:362/385 of 2hufA

query
sites
2hufA

L

L

F

M

I

G

P

P

G

G

P

P

T

S

N

N

I

A

P

P

E

Q

Q

R

I

V

R

L

Q

D

A

A

M

M

N

S

L

R

P

P

M

I

E

L

D

G

M
x
H

R

L

S

H

P

P

R

E

Y

-

P

-

E

-

L

-

T

T

L

L

P

K

L

I

F

M

A

D

D

D

V

I

K

K

K

E

-

G

-

V

-

R

-

Y

V

L

F

F

K

Q

N

T

R

N

N

N

G

I

R

A

V

T

F

F

I

C

Y

L

P

S

S

A

S

S

G

G

T

H

G

G

A

G

W

M

E

E

A

A

A

T

M

L

T

C

N

N

V

L

L

L

S

E

P

D

G

G

D

D

R

V

V

I

L

L

M

I

S

G

R

H

F

T

G

G

Q

H

F

W

S

G

H

D

L

R

W

S

V

A

D

D

M

M

A

A

E

T

R

R

L

Y

G

G

L

A

E

D

V

V

D

R

C

V

L

V

D

K

R

S

E

K

W

V

G

G

T

Q

G

S

V

L

P

S

V

L

D

D

L

E

Y

I

T

R

E

D

R

A

L

L

A

L

A

-

D

-

K

-

A

I

H

H

R

K

I

P

K

S

A

V

V

L

F

F

V

L

T

T

H

Q

N

G

E

D

T
x
S

A

S

T

T

G

G

V

V

T

L

S

Q

D

G

V

L

A

E

A

G

V

V

R

G

A

A

A

L

L

-

D

-

A

C

S

H

G

Q

H

H

P

N

A

C

L

L

F

I

V

V

D

D

G

T

V

V

S

A

S

S

I

L

G

G

S

G

I

A

D

P

F

M

R

F

Q

M

D

D

E

R

W

W

G

E

V

I

D

D

C

A

A

M

V

Y

S

T

G

G

S

S

Q

Q

K

K

G

V

F

L

M

G

L

A

P

P

A

P

G

G

L

I

G

T

F

P

L

V

S

S

V

F

S

S

Q

H

K

R

A

A

L

V

E

E

A

R

A

Y

K

K

T

R

A

-

R

R

H

N

M

T

R

K

C

V

Y

K

F

V

S

Y

F

Y

E

W

D

D

M

M

I

S

K

L

T

V

N

G

D

D

-

Y

-

W

-

G

-

C

-

F

-

G

-

R

T

P

G

R

Y

I

F
x
Y

P

H

Y

H

T

T

P

I

P

S

T

S

Q

T

L

L

H

Y

G

G

L

L

R

R

A

E

A

A

L

I

D

A

V

M

I

A

F

C

E

E

G

G

L

L

E

P

N

A

I

L

F

I

A

A

R

R

H

H

R

E

H

D

L

C

A

A

D

K

G

R

V

L

R

Y

A

R

A

G

V

L

S

Q

A

D

W

A

G

G

L

F

K

E

L

L

C

Y

A

A

T

-

E

D

P

P

K

K

W

D

Y

R

S

L

D

S

T

T

V

V

S

T

A

T

I

I

R

K

L

V

P

P

E

Q

G

G

I

V

D

D

G

W

V

L

K

K

V

A

I

A

R

Q

H

Y

A

A

F

M

D

K

T

T

Y

Y

N

L

T

V

S

E

L

I

G

S

S

G

G

G

L

L

S

G

K

P

V

T

A

A

G

G

K

Q

V

V

F

F

R
|
R

I

I

G

G

H

L

L

M

G

G

S

Q

binding 1-butanol: H45 (≠ M30), S155 (≠ T143), Y257 (≠ F241), R356 (= R341)

Q3LSM4 Alanine--glyoxylate aminotransferase; EC 2.6.1.44 from Aedes aegypti (Yellowfever mosquito) (Culex aegypti) (see paper)
32% identity, 86% coverage: 7:347/395 of query aligns to 22:362/393 of Q3LSM4

query
sites
Q3LSM4

L

L

F

M

I

G

P

P

G

G

P

P

T

S

N

N

I

A

P

P

E

Q

Q

R

I

V

R

L

Q

D

A

A

M

M

N

S

L

R

P

P

M

I

E

L

D

G

M

H

R

L

S

H

P

P

R

E

Y

-

P

-

E

-

L

-

T

T

L

L

P

K

L

I

F

M

A

D

D

D

V

I

K

K

K

E

-

G

-

V

-

R

-

Y

V

L

F

F

K

Q

N

T

R

N

N

N

G

I

R

A

V

T

F

F

I

C

Y

L

P

S

S

A

S
|
S

G
|
G

T
x
H

G

G

A

G

W

M

E

E

A

A

A

T

M

L

T

C

N

N

V

L

L

L

S

E

P

D

G

G

D

D

R

V

V

I

L

L

M

I

S

G

R

H

F

T

G

G

Q

H

F

W

S

G

H

D

L

R

W

S

V

A

D

D

M

M

A

A

E

T

R

R

L

Y

G

G

L

A

E

D

V

V

D

R

C

V

L

V

D

K

R

S

E

K

W

V

G

G

T

Q

G

S

V

L

P

S

V

L

D

D

L

E

Y

I

T

R

E

D

R

A

L

L

A

L

A

-

D

-

K

-

A

I

H

H

R

K

I

P

K

S

A

V

V

L

F

F

V

L

T

T

H

Q

N

G

E

D

T
x
S

A

S

T

T

G

G

V

V

T

L

S

Q

D

G

V

L

A

E

A

G

V

V

R

G

A

A

A

L

L

-

D

-

A

C

S

H

G

Q

H

H

P

N

A

C

L

L

F

I

V

V

D

D

G

T

V

V

S

A

S

S

I

L

G

G

S

G

I

A

D

P

F

M

R

F

Q

M

D

D

E

R

W

W

G

E

V

I

D

D

C

A

A

M

V

Y

S

T

G

G

S

S

Q
|
Q

K
|
K

G

V

F

L

M

G

L

A

P

P

A

P

G

G

L

I

G

T

F

P

L

V

S

S

V

F

S

S

Q

H

K

R

A

A

L

V

E

E

A

R

A

Y

K

K

T

R

A

-

R

R

H

N

M

T

R

K

C

V

Y

K

F

V

S

Y

F

Y

E

W

D

D

M

M

I

S

K

L

T

V

N

G

D

D

-

Y

-

W

-

G

-

C

-

F

-

G

-

R

T

P

G

R

Y

I

F
x
Y

P

H

Y

H

T
|
T

P

I

P

S

T

S

Q

T

L

L

H

Y

G

G

L

L

R

R

A

E

A

A

L

I

D

A

V

M

I

A

F

C

E

E

G

G

L

L

E

P

N

A

I

L

F

I

A

A

R

R

H

H

R

E

H

D

L

C

A

A

D

K

G

R

V

L

R

Y

A

R

A

G

V

L

S

Q

A

D

W

A

G

G

L

F

K

E

L

L

C

Y

A

A

T

-

E

D

P

P

K

K

W

D

Y

R

S

L

D

S

T

T

V

V

S

T

A

T

I

I

R

K

L

V

P

P

E

Q

G

G

I

V

D

D

G

W

V

L

K

K

V

A

I

A

R

Q

H

Y

A

A

F

M

D

K

T

T

Y

Y

N

L

T

V

S

E

L

I

G

S

S

G

G

G

L

L

S

G

K

P

V

T

A

A

G

G

K

Q

V

V

F

F

R
|
R

I

I

G

G

H

L

L

M

G

G

S

Q

SGH 78:80 (≠ SGT 63:65) binding in other chain
S155 (≠ T143) binding glyoxylate; binding L-alanine
Q205 (= Q195) binding in other chain
K206 (= K196) modified: N6-(pyridoxal phosphate)lysine
Y257 (≠ F241) binding pyridoxal 5'-phosphate
T260 (= T244) binding pyridoxal 5'-phosphate
R356 (= R341) binding glyoxylate

O32148 (S)-ureidoglycine--glyoxylate transaminase; UGXT; (S)-ureidoglycine--glyoxylate aminotransferase; Purine catabolism protein PucG; EC 2.6.1.112 from Bacillus subtilis (strain 168) (see paper)
29% identity, 98% coverage: 7:392/395 of query aligns to 14:415/416 of O32148

query
sites
O32148

L

I

F

M

I

T

P

P

G

G

P

P

T

V

N

E

I

V

P

D

E

P

Q

R

I

V

R

L

Q

R

A

V

M

M

N

S

L

T

P

P

M

V

E

V

D

G

M
x
Q

R

F

S

D

P

P

R

A

Y

F

P

T

E

G

L

I

T

M

L

N

P

E

L

T

F

M

A

E

D

M

V

L

K

R

K

E

V

L

F

F

K

Q

N

T

R

K

N

N

G

R

R

-

V

-

F

W

I

A

Y

Y

P

P

S

I

S

D

G

G

T

T

-

S

-

R

G

A

A

G

W

I

E

E

A

A

A

V

M

L

T

A

N

S

V

V

L

I

S

E

P

P

G

E

D

D

R

D

V

V

L

L

M

I

S

P

R

I

F

Y

G

G

Q

R

F

F

S

G

H

Y

L

L

W

L

V

T

D

E

M

I

A

A

E

E

R

R

L

Y

G

G

L

A

E

N

V

V

D

H

C

M

L

L

D

E

R

C

E

E

W

W

G

G

T

T

G

V

V

F

-

D

P

P

V

E

D

D

L

I

Y

I

T

R

E

E

R

-

L

-

A

-

D

I

K

K

A

K

H

V

R

K

I

P

K

K

A

I

V

V

F

A

V

M

T

V

H

H

N

G

E

E

T

T

A

S

T

T

G

G

V

R

T

I

S

H

D

P

V

L

A

K

A

A

V

I

R

G

A

E

A

A

L

-

D

-

A

C

S

R

G

T

H

E

P

D

A

A

L

L

L

F

F

I

V

V

D

D

G

A

V

V

S

A

S

T

I

I

G

G

S

G

I

C

D

E

F

V

R

K

Q

V

D

D

E

E

W

W

G

K

V

I

D

D

C

A

A

A

V

I

S

G

G

G

S

T

Q

Q

K
|
K

G

C

F

L

M

S

L

V

P

P

A

S

G

G

L

M

G

A

F

P

L

I

S

T

V

Y

S

N

Q

E

-

R

-

V

-

A

-

D

-

V

-

I

-

A

-

R

K

K

A

K

L

V

E

E

A

R

A

G

K

I

T

A

A

T

R

Q

H

A

M

D

R

R

C

A

Y

A

F

L

S

S

F

G

E

N

D

R

M

P

I

I

K

T

T

S

N

N

D

-

T

-

G

-

Y

Y

F

F

P

D

-

L

-

S

-

Q

-

L

-

E

-

D

-

Y

-

W

-

S

-

E

-

R

-

L

-
x
N

-

H

Y

H

T

T

P

E

P

A

T

T

Q

T

L

M

L

L

H

Y

G

A

L

L

R

R

A

E

A

G

L

V

D

R

V

L

I

V

F

L

E

E

G

G

L

L

E

E

N

T

I

R

F

F

A

E

R

R

H

H

R

R

H

H

L

H

A

E

D

A

G

A

V

L

R

A

A

A

A

G

V

I

S

K

A

A

W

M

G

G

L

L

K

R

L

L

C

F

A

G

T

D

E

D

P

-

K

S

W

C

Y

K

S

M

D

P

T

V

V

V

S

T

A

C

I

V

R

E

L

I

P

P

E

G

G

G

I

I

D

D

G

G

V

E

K

S

V

V

I

R

R

D

H

M

A

L

F

L

D

A

T

Q

Y

F

N

G

T

I

S

E

L

I

G

A

S

S

G

S

L

F

S

G

K

P

V

L

A

A

G

G

K

K

V

I

F

W

R

R

I

I

G

G

H

T

L

M

G

G

-

Y

S

S

L

C

N

R

E

K

V

E

M

N

V

V

L

L

G

F

A

V

L

L

S

A

A

G

A

L

E

E

L

A

T

V

L

L

D

R

C

H

G

N

V

A

K

G

I

I

E

E

P

A

G

G

A

K

G

A

V

L

G

Q

A

A

I

L

R

D

Q

V

F

Y

R

E

A

N

A

A

A

G

T

R

A

Q

T

A

A

A

Q37 (≠ M30) mutation to H: 5-fold decrease in transamination activity.
K198 (= K196) modified: N6-(pyridoxal phosphate)lysine
N264 (vs. gap) mutation to S: 9-fold decrease in transamination activity.; mutation to Y: Loss of transamination activity.

2dr1A Crystal structure of the ph1308 protein from pyrococcus horikoshii ot3
33% identity, 87% coverage: 6:349/395 of query aligns to 18:357/381 of 2dr1A

query
sites
2dr1A

H

K

L

L

F

F

I

T

P

A

G

G

P

P

T

V

N

A

I

C

P

F

E

P

Q

E

I

V

R

L

Q

E

A

I

M

M

N

K

L

V

P

Q

M

M

E

F

D

S

M

H

R

R

S

S

P

K

R

E

Y

Y

P

R

E

K

L

V

T

H

L

M

P

D

L

T

F

V

A

E

D

R

V

L

K

R

K

E

V

F

F

L

K

E

N

V

R

E

N

K

G

G

R

E

V

V

F

L

I

L

Y

V

P

P

S

S

S
|
S

G
|
G

T
|
T

G

G

A

I

W

M

E

E

A

A

A

S

M

I

T

R

N

N

V

G

L

V

S

S

P

K

G

G

D

G

R

K

V

V

L

L

M

V

S

T

R

I

F

I

G

G

Q

A

F
|
F

S

G

H

K

L

R

W

Y

V

K

D

E

M

V

A

V

E

E

R

S

L

N

G

G

L

R

E

K

V

A

D

V

C

V

L

L

D

E

R

Y

E

E

W

P

G

G

T

K

G

A

V

V

-

K

P

P

V

E

D

D

L

L

Y

D

T

D

E

-

R

-

L

-

A

A

A

L

D

R

K

K

A

N

H

P

R

D

I

V

K

E

A

A

V

V

F

T

V

I

T

T

H

Y

N

N

E

E

T
|
T

A

S

T

T

G

G

V

V

T

L

S

N

D

P

V

L

A

P

A

E

V

L

R

-

A

-

A

A

L

K

D

V

A

A

S

K

G

E

H

H

P

D

A

K

L

L

L

V

F

F

V

V

D
|
D

G

A

V
|
V

S

S

S

A

I

M

G

G

S

G

I

A

D

D

F

I

R

K

Q

F

D

D

E

K

W

W

G

G

V

L

D

D

C

V

A

V

V

F

S

S

G
x
S

S

S

Q

Q

K
|
K

G

A

F

F

M

G

L

V

P

P

A

P

G

G

L

L

G

A

F

I

L

G

S

A

V

F

S

S

Q

E

K

R

A

F

L

L

E

E

A

I

A

A

K

E

T

K

A

M

R

P

H

E

M

R

R

G

C

W

Y

Y

F

F

S

D

F

I

E

P

D

L

M

Y

I

V

K

K

-

Y

T

L

N

K

D

E

T

K

G

E

Y

S

F

T

P

P

Y

S

T

T

P

P

T

M

Q

P

L

Q

L

V

H

F

G

G

L

I

R

N

A

V

A

A

L

L

D

R

V

I

I

I

F

E

E

K

E

M

-

G

G

G

L

K

E

E

N

K

I

W

F

L

A

E

R

M

H

Y

R

E

H

K

L

R

A

A

D

K

G

M

V

V

R

R

A

E

A

G

V

V

S

R

A

E

W

I

G

G

L

L

K

D

L

I

C

L

A

A

T

-

E

E

P

P

K

G

W

H

Y

E

S

S

D

P

T

T

V

I

S

T

A

A

I

V

R

L

L

T

P

P

E

P

G

G

I

I

D

K

G

G

V

D

K

E

V

V

I

Y

R

-

H

E

A

A

F

M

D

R

T

K

Y

R

N

G

T

F

S

E

L

L

G

A

S

K

G

G

L

Y

S

G

K

S

V

V

A

K

G

E

K

K

V

T

F

F

R

R

I

I

G

G

H

H

L

M

G

G

S

Y

L

M

N

K

binding pyridoxal-5'-phosphate: S75 (= S63), G76 (= G64), T77 (= T65), F102 (= F90), T153 (= T143), D178 (= D170), V180 (= V172), S201 (≠ G193), K204 (= K196)

1j04A Structural mechanism of enzyme mistargeting in hereditary kidney stone disease in vitro (see paper)
32% identity, 87% coverage: 5:346/395 of query aligns to 19:362/387 of 1j04A

query
sites
1j04A

N

N

H

Q

L

L

F

L

I

L

-

G

P
|
P

G

G

P

P

T

S

N

N

I

L

P

P

E

P

Q

R

I

I

R

M

Q

A

A

A

M

G

N

G

L

L

P

Q

M

M

E

I

D

G

M

S

R

M

S

S

P

K

R

D

Y

M

P

Y

E

Q

L

I

T

M

L

D

P

E

L

I

F

K

A

E

D

G

V

I

K

Q

K

Y

V

V

F

F

K

Q

N

T

R

R

N

N

G

P

R

L

V

T

F

L

I

V

Y

I

P

S

S

G

S

S

G

G

T

H

G

C

A

A

W

L

E

E

A

A

M

L

T

V

N

N

V

V

L

L

S

E

P

P

G

G

D

D

R

S

V

F

L

L

M

V

S

G

R

A

F

N

G

G

Q

I

F

W

S

G

H

Q

L

R

W

A

V

V

D

D

M

I

A

G

E

E

R

R

L

I

G

G

L

A

E

R

V

V

D

H

C

P

L

M

D

T

R

K

E

D

W

P

G

G

T

G

G

H

V

Y

P

T

V

L

D

Q

L

E

Y

V

T

E

E

E

R

G

L

L

A

A

A

Q

D

-

K

-

A

-

H

H

R

K

I

P

K

V

A

L

V

L

F

F

V

L

T

T

H

H

N

G

E

E

T

S

A

S

T

T

G

G

V

V

T

L

S

Q

D

P

V

L

A

D

A

G

V

F

R

R

A

E

A

L

L

-

D

-

A

C

S

H

G

R

H

Y

P

K

A

C

L

L

F

L

V

V

D

D

G

S

V

V

S

A

S

S

I

L

G

G

S

G

I

T

D

P

F

L

R

Y

Q

M

D

D

E

R

W

Q

G

G

V

I

D

D

C

I

A

L

V

Y

S

S

G

G

S

S

Q

Q

K

K

G

A

F

L

M

N

L

A

P

P

A

P

G

G

L

T

G

S

F

L

L

I

S

S

V

F

S

S

Q

D

K

K

A

A

L

K

E

K

A

K

A

M

K

Y

T

S

A

R

R

K

H

T

M

K

R

P

C

-

Y

-

F

F

S

S

F

F

E

Y

D

L

M

D

I

I

K

K

-

W

-

L

-

A

-

N

-

F

-

W

-

G

-

C

T

D

N

D

D

Q

T

P

G

R

Y

M

F

Y

P

H

Y

H

T

T

P

I

P

P

T

V

Q

I

L

S

L

L

H

Y

G

S

L

L

R

R

A

E

A

S

L

L

D

A

V

L

I

I

F

A

E

E

E

Q

G

G

L

L

E

E

N

N

I

S

F

W

A

R

R

Q

H

H

R

R

H

E

L

A

A

A

D

A

G

Y

V

L

R

H

A

G

A

R

V

L

S

Q

A

A

W

L

G

G

L

L

K

Q

L

L

C

F

A

V

T

K

E

D

P

P

K

A

W

L

Y

R

S

L

D

P

T

T

V

V

S

T

A

T

I

V

R

A

L

V

P

P

E

A

G

G

I

Y

D

D

G

W

V

R

K

D

V

I

I

V

R

S

H

Y

A

V

F

I

D

D

T

H

Y

F

N

D

T

I

S

E

L

I

G

M

S

G

G

G

L

L

S

G

K

P

V

S

A

T

G

G

K

K

V

V

F

L

R
|
R

I

I

G

G

H

L

L

L

G

G

binding (aminooxy)acetic acid: P25 (= P10), R357 (= R341)

1vjoA Crystal structure of alanine--glyoxylate aminotransferase (alr1004) from nostoc sp. At 1.70 a resolution (see paper)
31% identity, 86% coverage: 7:346/395 of query aligns to 21:357/377 of 1vjoA

query
sites
1vjoA

L

L

F

L

I

G

P

P

G

G

P

P

T

S

N

N

I

A

P

H

E

P

Q

S

I

V

R

L

Q

Q

A

A

M

M

N

N

L

V

P

S

M

P

E

V

D

G

M

H

R

L

S

D

P

P

R

A

Y

F

P

L

E

A

L

L

T

M

L

D

P

E

L

I

F

Q

A

S

D

L

V

L

K

R

K

Y

V

V

F

W

K

Q

N

T

R

E

N

N

G

P

R

L

V

T

F

I

I

A

Y

V

P

S

S

G

S
x
T

G
|
G

T
|
T

G

A

A

A

W

M

E

E

A

A

A

T

M

I

T

A

N

N

V

A

L

V

S

E

P

P

G

G

D

D

R

V

V

V

L

L

M

I

S

G

R

V

F

A

G

G

Q

Y

F
|
F

S

G

H

N

L

R

W

L

V

V

D

D

M

M

A

A

E

G

R

R

L

Y

G

G

L

A

E

D

V

V

D

R

C

T

L

I

D

S

R

K

E

P

W

W

G

G

T

E

G

V

V

F

P

S

V

L

D

E

L

-

Y

-

T

-

E

E

R

L

L

R

A

T

A

A

D

L

K

E

A

T

H

H

R

R

I

P

K

A

A

I

V

L

F

A

V

L

T

V

H

H

N

A

E

E

T
|
T

A

S

T

T

G

G

V

A

T

R

S

Q

D

P

V

L

A

E

A

G

V

V

R

G

A

E

A

L

L

C

D

R

A

E

S

F

G

G

H

-

P

-

A

T

L

L

F

L

V

V

D
|
D

G

T

V
|
V

S

T

S

S

I

L

G

G

S

G

I

V

D

P

F

I

R

F

Q

L

D

D

E

A

W

W

G

G

V

V

D

D

C

L

A

A

V

Y

S

S

G

C

S

S

Q

Q

K
|
K

G

G

F

L

M

G

L

C

P

S

A

P

G

G

L

A

G

S

F

P

L

F

S

T

V

M

S

S

Q

S

K

R

A

A

L

I

E

E

A

K

A

L

K

Q

T

R

A

R

R

R

H

T

M

K

R

V

C

A

Y

N

F

W

S

Y

F

L

E

D

D

M

M

N

I

L

K

L

T

G

N

K

D

Y

T

W

G

G

-

S

-

E

-

R

Y

V

F

Y

P

H

Y

H

T

T

P

A

P

P

T

I

Q

N

L

L

L

Y

H

Y

G

A

L

L

R

R

A

E

A

A

L

L

D

R

V

L

I

I

F

A

E

Q

E

E

G

G

L

L

E

A

N

N

I

C

F

W

A

Q

R

R

H

H

R

Q

H

K

L

N

A

V

D

E

G

Y

V

L

R

W

A

E

A

R

V

L

S

E

A

D

W

I

G

G

L

L

K

S

L

L

C

-

A

H

T

V

E

E

P

K

K

E

W

Y

Y

R

S

L

D

P

T

T

V

L

S

T

A

T

I

V

R

C

L

I

P

P

E

D

G

G

I

V

D

D

G

G

V

K

K

A

V

V

I

A

R

R

H

R

A

L

F

L

D

N

T

E

Y

H

N

N

T

I

S

E

L

V

G

G

S

G

G

G

L

L

S

G

K

E

V

L

A

A

G

G

K

K

V

V

F

W

R

R

I

V

G

G

H

L

L

M

G

G

binding pyridoxal-5'-phosphate: T77 (≠ S63), G78 (= G64), T79 (= T65), F104 (= F90), T154 (= T143), D179 (= D170), V181 (= V172), K205 (= K196)

5hhyA Structure of human alanine:glyoxylate aminotransferase major allele (agt-ma) showing x-ray induced reduction of plp internal aldimine to 4'-deoxy-piridoxine-phosphate (plr) (see paper)
32% identity, 87% coverage: 5:346/395 of query aligns to 17:360/385 of 5hhyA

query
sites
5hhyA

N

N

H

Q

L

L

F

L

I

L

-

G

P

P

G

G

P

P

T

S

N

N

I

L

P

P

E

P

Q

R

I

I

R

M

Q

A

A

A

M

G

N

G

L

L

P

Q

M

M

E

I

D

G

M

S

R

M

S

S

P

K

R

D

Y

M

P

Y

E

Q

L

I

T

M

L

D

P

E

L

I

F

K

A

E

D

G

V

I

K

Q

K

Y

V

V

F

F

K

Q

N

T

R

R

N

N

G

P

R

L

V

T

F

L

I

V

Y

I

P

S

S

G

S
|
S

G
|
G

T
x
H

G

C

A

A

W

L

E

E

A

A

M

L

T

V

N

N

V

V

L

L

S

E

P

P

G

G

D

D

R

S

V

F

L

L

M

V

S

G

R

A

F

N

G

G

Q

I

F
x
W

S

G

H

Q

L

R

W

A

V

V

D

D

M

I

A

G

E

E

R

R

L

I

G

G

L

A

E

R

V

V

D

H

C

P

L

M

D

T

R

K

E

D

W

P

G

G

T

G

G

H

V

Y

P

T

V

L

D

Q

L

E

Y

V

T

E

E

E

R

G

L

L

A

A

A

Q

D

-

K

-

A

-

H

H

R

K

I

P

K

V

A

L

V

L

F

F

V

L

T

T

H

H

N

G

E

E

T
x
S

A

S

T

T

G

G

V

V

T

L

S

Q

D

-

V

-

A

-

V

-

R

-

A

-

A

P

L

L

D

D

A

G

S

F

G

G

-

E

-

L

-

C

-

H

-

R

H

Y

P

K

A

C

L

L

F

L

V

V

D
|
D

G

S

V
|
V

S

A

S

S

I

L

G

G

S

G

I

T

D

P

F

L

R

Y

Q

M

D

D

E

R

W

Q

G

G

V

I

D

D

C

I

A

L

V

Y

S

S

G

G

S

S

Q
|
Q

K
|
K

G

A

F

L

M

N

L

A

P

P

A

P

G

G

L

T

G

S

F

L

L

I

S

S

V

F

S

S

Q

D

K

K

A

A

L

K

E

K

A

K

A

M

K

Y

T

S

A

R

R

K

H

T

M

K

R

P

C

-

Y

-

F

F

S

S

F

F

E

Y

D

L

M

D

I

I

K

K

-

W

-

L

-

A

-

N

-

F

-

W

-

G

-

C

T

D

N

D

D

Q

T

P

G

R

Y

M

F
x
Y

P

H

Y

H

T
|
T

P

I

P

P

T

V

Q

I

L

S

L

L

H

Y

G

S

L

L

R

R

A

E

A

S

L

L

D

A

V

L

I

I

F

A

E

E

E

Q

G

G

L

L

E

E

N

N

I

S

F

W

A

R

R

Q

H

H

R

R

H

E

L

A

A

A

D

A

G

Y

V

L

R

H

A

G

A

R

V

L

S

Q

A

A

W

L

G

G

L

L

K

Q

L

L

C

F

A

V

T

K

E

D

P

P

K

A

W

L

Y

R

S

L

D

P

T

T

V

V

S

T

A

T

I

V

R

A

L

V

P

P

E

A

G

G

I

Y

D

D

G

W

V

R

K

D

V

I

I

V

R

S

H

Y

A

V

F

I

D

D

T

H

Y

F

N

D

T

I

S

E

L

I

G

M

S

G

G

G

L

L

S

G

K

P

V

S

A

T

G

G

K

K

V

V

F

L

R

R

I

I

G

G

H

L

L

L

G

G

binding (5-hydroxy-4,6-dimethylpyridin-3-yl)methyl dihydrogen phosphate: S76 (= S63), G77 (= G64), H78 (≠ T65), W103 (≠ F90), S153 (≠ T143), D178 (= D170), V180 (= V172), Q203 (= Q195), K204 (= K196), Y255 (≠ F241), T258 (= T244)

P21549 Alanine--glyoxylate aminotransferase; AGT; Serine--pyruvate aminotransferase; SPT; EC 2.6.1.44; EC 2.6.1.51 from Homo sapiens (Human) (see 24 papers)
32% identity, 87% coverage: 5:346/395 of query aligns to 22:365/392 of P21549

query
sites
P21549

N

N

H

Q

L

L

F

L

I

L

-

G

P

P

G

G

P

P

T

S

N

N

I

L

P

P

E

P

Q
x
R

I

I

R

M

Q

A

A

A

M
x
G

N

G

L

L

P

Q

M

M

E

I

D
x
G

M

S

R

M

S

S

P

K

R

D

Y

M

P

Y

E

Q

L

I

T

M

L

D

P

E

L

I

F

K

A

E

D

G

V

I

K

Q

K

Y

V

V

F

F

K

Q

N

T

R

R

N

N

G

P

R

L

V

T

F

L

I

V

Y

I

P

S

S

G

S

S

G
|
G

T

H

G

C

A

A

W

L

E

E

A

A

M

L

T

V

N

N

V

V

L

L

S

E

P

P

G

G

D

D

R

S

V

F

L

L

M

V

S

G

R

A

F

N

G

G

Q

I

F
x
W

S

G

H

Q

L

R

W
x
A

V

V

D

D

M

I

A

G

E

E

R

R

L

I

G

G

L

A

E

R

V

V

D

H

C

P

L

M

D

T

R

K

E

D

W

P

G

G

T

G

G

H

V

Y

P

T

V

L

D

Q

L

E

Y

V

T

E

E

E

R

G

L

L

A

A

A

Q

D

-

K

-

A

-

H

H

R

K

I

P

K

V

A
x
L

V

L

F
|
F

V

L

T

T

H

H

N
x
G

E

E

T
x
S

A

S

T

T

G
|
G

V

V

T

L

S

Q

D

-

V

-

A

-

V

-

R

-

A

-

A

P

L
|
L

D

D

A

G

S

F

G
|
G

-

E

-

L

-
x
C

-

H

-

R

H

Y

P

K

A

C

L

L

F

L

V

V

D
|
D

G

S

V

V

S

A

S
|
S

I

L

G

G

S

G

I

T

D

P

F

L

R

Y

Q

M

D

D

E

R

W

Q

G

G

V

I

D

D

C
x
I

A

L

V

Y

S
|
S

G

G

S

S

Q

Q

K
|
K

G

A

F

L

M

N

L

A

P

P

A

P

G

G

L

T

G
x
S

F

L

L

I

S

S

V

F

S

S

Q

D

K

K

A

A

L

K

E

K

A

K

A

M

K

Y

T

S

A
x
R

R

K

H

T

M

K

R

P

C

-

Y

-

F

F

S

S

F

F

E

Y

D

L

M

D

I
|
I

K

K

-

W

-

L

-

A

-

N

-

F

-

W

-

G

-
x
C

T

D

N

D

D

Q

T

P

G

R

Y

M

F

Y

P

H

Y

H

T

T

P

I

P

P

T

V

Q

I

L

S

L

L

H

Y

G

S

L

L

R

R

A

E

A

S

L

L

D

A

V

L

I
|
I

F
x
A

E

E

E

Q

G

G

L

L

E

E

N

N

I

S

F

W

A

R

R

Q

H

H

R

R

H

E

L

A

A

A

D

A

G

Y

V

L

R

H

A

G

A

R

V

L

S

Q

A

A

W

L

G

G

L

L

K

Q

L

L

C

F

A

V

T

K

E

D

P

P

K

A

W

L

Y

R

S

L

D

P

T

T

V

V

S

T

A

T

I

V

R

A

L
x
V

P

P

E

A

G

G

I

Y

D

D

G

W

V

R

K

D

V

I

I

V

R

S

H

Y

A

V

F
x
I

D

D

T

H

Y

F

N

D

T

I

S

E

L

I

G

M

S

G

G

G

L

L

S

G

K

P

V

S

A

T

G

G

K

K

V

V

F

L

R

R

I

I

G

G

H

L

L

L

G

G

R36 (≠ Q18) to C: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177157
G41 (≠ M23) to E: in HP1; loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177168; to R: in HP1; associated in cis with L-11 and M-340; results in loss of protein stability; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; partial mitochondrial mistargeting; intraperoxisomal protein aggregation seen; dbSNP:rs121908523; to V: in HP1; reduced alanine--glyoxylate aminotransferase activity; no loss of dimerization; no effect on protein stability; dbSNP:rs180177168
G47 (≠ D29) to R: in HP1; associated in cis with L-11 and M-340; results in protein misfolding; decreased alanine--glyoxylate aminotransferase activity; reduced expression levels; reduced pyridoxal phosphate binding; reduced dimerization; reduced thermostability; increased propensity to aggregation; increased susceptibility to proteolytic degradation within the N-terminal region; mitochondrial mistargeting; exposure to pyridoxine can rescue the functionality by partially preventing aggregation and degradation and by redirecting all the protein to the peroxisome; dbSNP:rs180177173
G82 (= G64) to E: in HP1; abolishes alanine--glyoxylate aminotransferase activity by interfering with pyridoxal phosphate binding; dbSNP:rs121908522
W108 (≠ F90) to R: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; dbSNP:rs180177197
A112 (≠ W94) to D: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; causes protein aggregation; dbSNP:rs796052061
L150 (≠ A135) to P: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177222
F152 (= F137) to I: in HP1; associated in cis with L-11 and M-340; results in protein destabilization; no loss of dimerization; decreased alanine--glyoxylate aminotransferase activity; loss of alanine--glyoxylate aminotransferase activity when associated with L-11 and M-340; mitochondrial mistargeting when associated with L-11 and M-340; dbSNP:rs121908524
G156 (≠ N141) to R: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; dbSNP:rs121908530
S158 (≠ T143) to L: in HP1; loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177225
G161 (= G146) to C: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; reduced peroxisomal localization; dbSNP:rs180177227; to R: in HP1; loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; loss of dimerization; dbSNP:rs180177227; to S: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; reduced peroxisomal localization; dbSNP:rs180177227
L166 (= L158) to P: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177230
G170 (= G162) to R: in HP1; associated in cis with L-11 and M-340; decrease in alanine--glyoxylate aminotransferase activity; loss of dimerization; partial loss of protein stability but protein stability increases in the presence of pyridoxal phosphate; causes protein aggregation; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and M-340; results in mitochondrial mistargeting when associated with L-11 and M-340; dbSNP:rs121908529
C173 (vs. gap) to Y: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; causes protein aggregation; dbSNP:rs180177231
D183 (= D170) to N: in HP1; loss of alanine--glyoxylate aminotransferase activity; no loss of dimerization; no effect on protein stability; dbSNP:rs180177236
S187 (= S174) to F: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization but improved dimerization in the presence of pyridoxal phosphate; decreased protein stability; dbSNP:rs180177238
I202 (≠ C189) to N: in HP1; uncertain significance; dbSNP:rs536352238
S205 (= S192) to P: in HP1; loss of alanine--glyoxylate aminotransferase activity; decreased protein stability; dbSNP:rs121908520
K209 (= K196) mutation to R: Affects pyridoxal phosphate binding; loss of alanine--glyoxylate aminotransferase activity.
S218 (≠ G205) to L: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; no effect on protein stability; dbSNP:rs180177253
R233 (≠ A220) to C: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs121908526; to H: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs121908527
I244 (= I233) to T: in HP1; associated in cis with L-11 and M-340; results in protein misfolding; decreased alanine--glyoxylate aminotransferase activity in vitro; no loss of dimerization; partial mitochondrial mistargeting; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and M-340; dbSNP:rs121908525
C253 (vs. gap) to R: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177264
I279 (= I260) to T: in dbSNP:rs140992177
A280 (≠ F261) to V: in dbSNP:rs73106685
V326 (≠ L307) to I: in dbSNP:rs115057148
I340 (≠ F321) to M: in allele minor; associated in cis with L-11; no effect on alanine--glyoxylate aminotransferase activity in vitro; decreased specific alanine--glyoxylate aminotransferase activity in vitro when associated with L-11; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and I-152; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and R-170; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and T-244; results in mitochondrial mistargeting when associated with L-11 and R-170; dbSNP:rs4426527

Sites not aligning to the query:

9 T → N: no loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs115014558
11 P → L: in allele minor; associated in cis with M-340; decreased specific alanine--glyoxylate aminotransferase activity in vitro when associated with M-340; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with I-152 and M-340; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with R-170 and M-340; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with T-244 and M-340; causes mitochondrial mistargeting when associated with R-170 and M-340; dbSNP:rs34116584

6rv0A Human alanine:glyoxylate aminotransferase major allele (agt-ma); with pmp in the active site (see paper)
32% identity, 87% coverage: 5:346/395 of query aligns to 17:360/384 of 6rv0A

query
sites
6rv0A

N

N

H

Q

L

L

F

L

I

L

-

G

P

P

G

G

P

P

T

S

N

N

I

L

P

P

E

P

Q

R

I

I

R

M

Q

A

A

A

M

G

N

G

L

L

P

Q

M

M

E

I

D

G

M

S

R

M

S

S

P

K

R

D

Y

M

P

Y

E

Q

L

I

T

M

L

D

P

E

L

I

F

K

A

E

D

G

V

I

K

Q

K

Y

V

V

F

F

K

Q

N

T

R

R

N

N

G

P

R

L

V

T

F

L

I

V

Y

I

P

S

S

G

S
|
S

G
|
G

T
x
H

G

C

A

A

W

L

E

E

A

A

M

L

T

V

N

N

V

V

L

L

S

E

P

P

G

G

D

D

R

S

V

F

L

L

M

V

S

G

R

A

F

N

G

G

Q

I

F
x
W

S

G

H

Q

L

R

W

A

V

V

D

D

M

I

A

G

E

E

R

R

L

I

G

G

L

A

E

R

V

V

D

H

C

P

L

M

D

T

R

K

E

D

W

P

G

G

T

G

G

H

V

Y

P

T

V

L

D

Q

L

E

Y

V

T

E

E

E

R

G

L

L

A

A

A

Q

D

-

K

-

A

-

H

H

R

K

I

P

K

V

A

L

V

L

F

F

V

L

T

T

H

H

N

G

E

E

T

S

A

S

T

T

G

G

V

V

T

L

S

Q

D

-

V

-

A

-

V

-

R

-

A

-

A

P

L

L

D

D

A

G

S

F

G

G

-

E

-

L

-

C

-

H

-

R

H

Y

P

K

A

C

L

L

F

L

V

V

D
|
D

G

S

V
|
V

S
x
A

S

S

I

L

G

G

S

G

I

T

D

P

F

L

R

Y

Q

M

D

D

E

R

W

Q

G

G

V

I

D

D

C

I

A

L

V

Y

S

S

G

G

S

S

Q

Q

K
|
K

G

A

F

L

M

N

L

A

P

P

A

P

G

G

L

T

G

S

F

L

L

I

S

S

V

F

S

S

Q

D

K

K

A

A

L

K

E

K

A

K

A

M

K

Y

T

S

A

R

R

K

H

T

M

K

R

P

C

-

Y

-

F

F

S

S

F

F

E

Y

D

L

M

D

I

I

K

K

-

W

-

L

-

A

-

N

-

F

-

W

-

G

-

C

T

D

N

D

D

Q

T

P

G

R

Y

M

F

Y

P

H

Y

H

T

T

P

I

P

P

T

V

Q

I

L

S

L

L

H

Y

G

S

L

L

R

R

A

E

A

S

L

L

D

A

V

L

I

I

F

A

E

E

E

Q

G

G

L

L

E

E

N

N

I

S

F

W

A

R

R

Q

H

H

R

R

H

E

L

A

A

A

D

A

G

Y

V

L

R

H

A

G

A

R

V

L

S

Q

A

A

W

L

G

G

L

L

K

Q

L

L

C

F

A

V

T

K

E

D

P

P

K

A

W

L

Y

R

S

L

D

P

T

T

V

V

S

T

A

T

I

V

R

A

L

V

P

P

E

A

G

G

I

Y

D

D

G

W

V

R

K

D

V

I

I

V

R

S

H

Y

A

V

F

I

D

D

T

H

Y

F

N

D

T

I

S

E

L

I

G

M

S

G

G

G

L

L

S

G

K

P

V

S

A

T

G

G

K

K

V

V

F

L

R

R

I

I

G

G

H

L

L

L

G

G

binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: S76 (= S63), G77 (= G64), H78 (≠ T65), W103 (≠ F90), D178 (= D170), V180 (= V172), A181 (≠ S173), K204 (= K196)

1h0cA The crystal structure of human alanine:glyoxylate aminotransferase (see paper)
32% identity, 87% coverage: 5:346/395 of query aligns to 19:360/385 of 1h0cA

query
sites
1h0cA

N

N

H

Q

L

L

F

L

I

L

-

G

P
|
P

G
|
G

P

P

T

S

N

N

I

L

P

P

E

P

Q

R

I

I

R

M

Q

A

A

A

M

G

N

G

L

L

P

Q

M

M

E

I

D

G

M

S

R

M

S

S

P

K

R

D

Y

M

P

Y

E

Q

L

I

T

M

L

D

P

E

L

I

F

K

A

E

D

G

V

I

K

Q

K

Y

V

V

F

F

K

Q

N

T

R

R

N

N

G

P

R

L

V

T

F

L

I

V

Y

I

P

S

S

G

S
|
S

G
|
G

T
x
H

G

C

A

A

W

L

E

E

A

A

M

L

T

V

N

N

V

V

L

L

S

E

P

P

G

G

D

D

R

S

V

F

L

L

M

V

S

G

R

A

F

N

G

G

Q

I

F
x
W

S

G

H

Q

L

R

W

A

V

V

D

D

M

I

A

G

E

E

R

R

L

I

G

G

L

-

E

-

V

V

D

H

C

P

L

M

D

T

R

K

E

D

W

P

G

G

T

G

G

H

V

Y

P

T

V

L

D

Q

L

E

Y

V

T

E

E

E

R

G

L

L

A

A

A

Q

D

-

K

-

A

-

H

H

R

K

I

P

K

V

A

L

V

L

F

F

V

L

T

T

H

H

N

G

E

E

T
x
S

A

S

T

T

G

G

V

V

T

L

S

Q

D

-

V

-

A

-

V

-

R

-

A

-

A

P

L

L

D

D

A

G

S

F

G

G

-

E

-

L

-

C

-

H

-

R

H

Y

P

K

A

C

L

L

F

L

V

V

D
|
D

G

S

V
|
V

S

A

S

S

I

L

G

G

S

G

I

T

D

P

F

L

R

Y

Q

M

D

D

E

R

W

Q

G

G

V

I

D

D

C

I

A

L

V

Y

S

S

G

G

S

S

Q

Q

K
|
K

G

A

F

L

M

N

L

A

P

P

A

P

G

G

L

T

G

S

F

L

L

I

S

S

V

F

S

S

Q

D

K

K

A

A

L

K

E

K

A

K

A

M

K

Y

T

S

A

R

R

K

H

T

M

K

R

P

C

-

Y

-

F

F

S

S

F

F

E

Y

D

L

M

D

I

I

K

K

-

W

-

L

-

A

-

N

-

F

-

W

-

G

-

C

T

D

N

D

D

Q

T

P

G

R

Y

M

F

Y

P

H

Y

H

T

T

P

I

P

P

T

V

Q

I

L

S

L

L

H

Y

G

S

L

L

R

R

A

E

A

S

L

L

D

A

V

L

I

I

F

A

E

E

E

Q

G

G

L

L

E

E

N

N

I

S

F

W

A

R

R

Q

H

H

R

R

H

E

L

A

A

A

D

A

G

Y

V

L

R

H

A

G

A

R

V

L

S

Q

A

A

W

L

G

G

L

L

K

Q

L

L

C

F

A

V

T

K

E

D

P

P

K

A

W

L

Y

R

S

L

D

P

T

T

V

V

S

T

A

T

I

V

R

A

L

V

P

P

E

A

G

G

I

Y

D

D

G

W

V

R

K

D

V

I

I

V

R

S

H

Y

A

V

F

I

D

D

T

H

Y

F

N

D

T

I

S

E

L

I

G

M

S

G

G

G

L
|
L

S

G

K

P

V

S

A

T

G

G

K

K

V

V

F

L

R
|
R

I

I

G

G

H

L

L

L

G

G

binding (aminooxy)acetic acid: P25 (= P10), G26 (= G11), L346 (= L332), R355 (= R341)
binding pyridoxal-5'-phosphate: S78 (= S63), G79 (= G64), H80 (≠ T65), W105 (≠ F90), S153 (≠ T143), D178 (= D170), V180 (= V172), K204 (= K196)

Sites not aligning to the query:

binding pyridoxal-5'-phosphate: 385

2bkwA Yeast alanine:glyoxylate aminotransferase yfl030w (see paper)
31% identity, 86% coverage: 7:346/395 of query aligns to 6:357/381 of 2bkwA

query
sites
2bkwA

L

L

F

L

I

I

P

P

G

G

P

P

T

I

N

I

I

L

P

S

E

G

Q

A

I

V

R

Q

Q

K

A

A

M

L

N

D

L

V

P

P

M

S

E

L

D

G

M

H

R

T

S

S

P

P

R

E

Y

F

P

V

E

S

L

I

T

F

L

Q

P

R

L

V

F

L

A

K

D

N

V

T

K

R

K

A

V

V

F

F

K

K

N

S

-

A

R

S

N

K

G

S

R

Q

V

P

F

F

I

V

Y

L

P

A

S

G

S
|
S

G
|
G

T
|
T

G

L

A

G

W

W

E

D

A

I

A

F

M

A

T

S

N

N

V

F

L

I

-

L

-

S

-

K

S

A

P

P

G

N

D

K

R

N

V

V

L

L

M

V

S

V

R

S

F

T

G

G

Q

T

F
|
F

S

S

H

D

L

R

W

F

V

A

D

D

M

C

A

L

E

R

R

S

L

Y

G

G

L

A

E

Q

V

V

D

D

C

V

L

V

-

R

D

P

R

L

E

K

W

I

G

G

T

E

G

S

V

V

P

P

V

L

D

E

L

L

Y

I

T

T

E

E

R

K

L

L

A

S

A

Q

D

N

K

S

A

-

H

-

R

-

I

Y

K

G

A

A

V

V

F

T

V

V

T

T

H

H

N

V

E

D

T
|
T

A

S

T

T

G

A

V

V

T

L

S

S

D

D

V

L

A

K

A

A

V

I

R

S

A

Q

A

A

L

I

D

K

A

Q

S

T

G

S

H

P

P

E

A

T

L

F

F

V

V

V

D
|
D

G

A

V
|
V

S

C

S

S

I

I

G

G

S

C

I

E

D

E

F

F

R

E

Q

F

D

D

E

E

W

W

G

G

V

V

D

D

C

F

A

A

V

L

S

T

G

A

S

S

Q

Q

K
|
K

G

A

F

I

M

G

L

A

P

P

A

A

G

G

L

L

G

S

F

I

L

S

S

L

V

C

S

S

Q

S

K

R

-

F

-

M

-

D

-

Y

A

A

L

L

E

N

A

D

A

S

K

K

T

N

A

G

R

-

H

H

M

V

R

H

C

G

Y

Y

F

F

S

S

-

S

-

L

-

R

F

W

E

T

D

P

M

I

I

M

K

E

T

N

N

Y

D

E

T

A

G

G

Y

K

F

G

P

A

Y

Y

-

F

-

A

T

T

P

P

T

V

Q

Q

L

L

L

I

H

N

G

S

L

L

R

D

A

V

A

A

L

L

D

K

V

E

I

I

F

L

E

E

G

G

L

L

E

H

N

K

I

R

F

W

A

D

R

L

H

H

R

R

H

E

L

M

A

S

D

D

G

W

V

F

R

K

A

D

A

S

V

L

S

V

A

N

W

-

G

G

L

L

K

Q

L

L

C

-

A

-

T

T

E

S

P

V

K

S

W

R

Y

Y

S

P

D

S

T

N

V

M

S

S

A

A

I

-

R

-

L

-

P

-

E

H

G

G

I

L

D

T

G

A

V

V

K

Y

V

V

-

A

-

D

-

P

-

D

I

V

R

I

H

A

A

F

F

L

D

K

T

S

Y

H

N

G

T

V

S

V

L

I

G

A

S

G

G

G

L

I

S

H

K

K

V

D

A

I

G

G

-

P

K

K

V

Y

F

I

R

R

I

I

G

G

H

H

L

M

G

G

binding pyridoxal-5'-phosphate: S65 (= S63), G66 (= G64), T67 (= T65), F95 (= F90), T146 (= T143), D173 (= D170), V175 (= V172), K199 (= K196)

Sites not aligning to the query:

binding pyridoxal-5'-phosphate: 381

2ch1A Structure of anopheles gambiae 3-hydroxykynurenine transaminase (see paper)
29% identity, 87% coverage: 10:351/395 of query aligns to 23:365/388 of 2ch1A

query
sites
2ch1A

P

P

G

G

P

P

T

S

N

N

I

C

P

S

E

K

Q

R

I

V

R

L

Q

T

A

A

M

M

N

T

L

-

P

-

M

-

E

N

D

T

M

V

R

L

S

S

P

N

R

F

Y

H

P

A

E

E

L

L

-

F

-

R

T

T

L

M

P

D

L

E

F

V

A

K

D

D

-

G

V

L

K

R

K

Y

V

I

F

F

K

Q

N

T

R

E

N

N

G

R

R

A

V

T

F

M

I

C

Y

V

P

S

S

G

S
|
S

G
x
A

T
x
H

G

A

A

G

W

M

E

E

A

A

A

M

M

L

T

S

N

N

V

L

L

L

S

E

P

E

G

G

D

D

R

R

V

V

L

L

M

I

S

A

R

V

F

N

G

G

Q

I

F
x
W

S

A

H

E

L

R

W

A

V

V

D

E

M

M

A

S

E

E

R

R

L

Y

G

G

L

A

E

D

V

V

D

R

C

T

L

I

D

E

R

-

E

-

W

-

G

-

T

-

G

G

V

P

P

P

V

D

D

R

L

P

Y

F

T

S

-

L

E

E

R

T

L

L

A

A

-

R

A

A

D

I

K

E

A

L

H

H

R

Q

I

P

K

K

A

C

V

L

F

F

V

L

T

T

H

H

N

G

E

D

T
x
S

A

S

T

S

G

G

V

L

T

L

S

Q

D

P

V

L

A

E

A

G

V

V

R

G

A

Q

A

I

L

-

D

-

A

C

S

H

G

Q

H

H

P

D

A

C

L

L

F

I

V

V

D
|
D

G

A

V
|
V

S

A

S

S

I

L

G

C

S

G

I

V

D

P

F

F

R

Y

Q

M

D

D

E

K

W

W

G

E

V

I

D

D

C

A

A

V

V

Y

S

T

G

G

S

A

Q
|
Q

K
|
K

G

V

F

L

M

G

L

A

P

P

A

P

G

G

L

I

G

T

F

P

L

I

S

S

V

I

S

S

Q

P

K

K

A

A

L

L

E

D

A

V

A

I

K

R

T

N

A

R

R

R

H

T

M

K

R

S

C

K

Y

V

F

F

S

Y

F

W

E

D

D

L

M

L

I

L

K

L

T

G

N

N

D

Y

T

W

G

G

Y

C

F

Y

P

D

-

E

-

P

-

K

-

R

-
x
Y

-

H

Y

H

T
|
T

P

V

P

A

T

S

Q

N

L

L

L

I

H

F

G

A

L

L

R

R

A

E

A

A

L

L

D

A

V

Q

I

I

F

A

E

E

G

G

L

L

E

E

N

N

I

Q

F

I

A

K

R

R

H

R

R

I

H

E

L

C

A

A

D

Q

G

I

V

L

R

Y

A

E

A

G

V

L

S

G

A

K

W

M

G

G

L

L

K

D

L

I

C

F

A

V

T

K

E

D

P

P

K

R

W

H

Y

R

S

L

D

P

T

T

V

V

S

T

A

G

I

I

R

M

L

I

P

P

E

K

G

G

I

V

D

D

G

W

V

W

K

K

V

V

I

S

R

Q

H

Y

A

A

F

M

D

N

T

N

Y

F

N

S

T

L

S

E

L

V

G

Q

S

G

G

G

L

L

S

G

K

P

V

T

A

F

G

G

K

K

V

A

F

W

R

R

I

V

G

G

H

I

L

M

G

G

S

E

L

C

N

S

E

T

V

V

binding pyridoxal-5'-phosphate: S76 (= S63), A77 (≠ G64), H78 (≠ T65), W103 (≠ F90), S153 (≠ T143), D178 (= D170), V180 (= V172), Q203 (= Q195), K204 (= K196), Y255 (vs. gap), T258 (= T244)

2ch2A Structure of the anopheles gambiae 3-hydroxykynurenine transaminase in complex with inhibitor (see paper)
29% identity, 87% coverage: 10:351/395 of query aligns to 22:364/387 of 2ch2A

query
sites
2ch2A

P

P

G
|
G

P

P

T

S

N

N

I

C

P

S

E

K

Q

R

I

V

R

L

Q

T

A

A

M

M

N

T

L

-

P

-

M

-

E

N

D

T

M

V

R

L

S
|
S

P
x
N

R

F

Y

H

P

A

E

E

L

L

-

F

-

R

T

T

L

M

P

D

L

E

F

V

A

K

D

D

-

G

V

L

K

R

K

Y

V

I

F

F

K

Q

N

T

R

E

N

N

G

R

R

A

V

T

F

M

I

C

Y

V

P

S

S

G

S
|
S

G
x
A

T
x
H

G

A

A

G

W

M

E

E

A

A

A

M

M

L

T

S

N

N

V

L

L

L

S

E

P

E

G

G

D

D

R

R

V

V

L

L

M

I

S

A

R

V

F

N

G

G

Q

I

F
x
W

S

A

H

E

L

R

W

A

V

V

D

E

M

M

A

S

E

E

R

R

L

Y

G

G

L

A

E

D

V

V

D

R

C

T

L

I

D

E

R

-

E

-

W

-

G

-

T

-

G

G

V

P

P

P

V

D

D

R

L

P

Y

F

T

S

-

L

E

E

R

T

L

L

A

A

-

R

A

A

D

I

K

E

A

L

H

H

R

Q

I

P

K

K

A

C

V

L

F

F

V

L

T

T

H

H

N

G

E

D

T
x
S

A

S

T

S

G

G

V

L

T

L

S

Q

D

P

V

L

A

E

A

G

V

V

R

G

A

Q

A

I

L

-

D

-

A

C

S

H

G

Q

H

H

P

D

A

C

L

L

F

I

V

V

D
|
D

G

A

V
|
V

S

A

S

S

I

L

G

C

S

G

I

V

D

P

F

F

R

Y

Q

M

D

D

E

K

W

W

G

E

V

I

D

D

C

A

A

V

V

Y

S

T

G

G

S

A

Q

Q

K
|
K

G

V

F

L

M

G

L

A

P

P

A

P

G

G

L

I

G

T

F

P

L

I

S

S

V

I

S

S

Q

P

K

K

A

A

L

L

E

D

A

V

A

I

K

R

T

N

A

R

R

R

H

T

M

K

R

S

C

K

Y

V

F

F

S

Y

F

W

E

D

D

L

M

L

I

L

K

L

T

G

N

N

D

Y

T

W

G

G

Y

C

F

Y

P

D

-

E

-

P

-

K

-

R

-
x
Y

-

H

Y

H

T
|
T

P

V

P

A

T

S

Q

N

L

L

L

I

H

F

G

A

L

L

R

R

A

E

A

A

L

L

D

A

V

Q

I

I

F

A

E

E

G

G

L

L

E

E

N

N

I

Q

F

I

A

K

R

R

H

R

R

I

H

E

L

C

A

A

D

Q

G

I

V

L

R

Y

A

E

A

G

V

L

S

G

A

K

W

M

G

G

L

L

K

D

L

I

C

F

A

V

T

K

E

D

P

P

K

R

W

H

Y

R

S

L

D

P

T

T

V

V

S

T

A

G

I

I

R

M

L

I

P

P

E

K

G

G

I

V

D

D

G

W

V

W

K

K

V

V

I

S

R

Q

H

Y

A

A

F

M

D

N

T

N

Y

F

N

S

T

L

S

E

L

V

G
x
Q

S

G

G

G

L
|
L

S

G

K

P

V

T

A

F

G

G

K

K

V

A

F

W

R
|
R

I

V

G

G

H

I

L

M

G

G

S

E

L

C

N

S

E

T

V

V

binding 4-(2-aminophenyl)-4-oxobutanoic acid: G23 (= G11), S41 (= S32), N42 (≠ P33), S152 (≠ T143), Y254 (vs. gap), Q342 (≠ G329), L345 (= L332), R354 (= R341)
binding pyridoxal-5'-phosphate: S75 (= S63), A76 (≠ G64), H77 (≠ T65), W102 (≠ F90), S152 (≠ T143), D177 (= D170), V179 (= V172), K203 (= K196), Y254 (vs. gap), T257 (= T244)

Q7PRG3 3-hydroxykynurenine transaminase; AgHKT; Alanine--glyoxylate aminotransferase; EC 2.6.1.63; EC 2.6.1.44 from Anopheles gambiae (African malaria mosquito) (see paper)
29% identity, 87% coverage: 10:351/395 of query aligns to 24:366/396 of Q7PRG3

query
sites
Q7PRG3

P

P

G

G

P

P

T

S

N

N

I

C

P

S

E

K

Q

R

I

V

R

L

Q

T

A

A

M

M

N

T

L

-

P

-

M

-

E

N

D

T

M

V

R

L

S

S

P

N

R

F

Y

H

P

A

E

E

L

L

-

F

-

R

T

T

L

M

P

D

L

E

F

V

A

K

D

D

-

G

V

L

K

R

K

Y

V

I

F

F

K

Q

N

T

R

E

N

N

G

R

R

A

V

T

F

M

I

C

Y

V

P

S

S

G

S
|
S

G
x
A

T
x
H

G

A

A

G

W

M

E

E

A

A

A

M

M

L

T

S

N

N

V

L

L

L

S

E

P

E

G

G

D

D

R

R

V

V

L

L

M

I

S

A

R

V

F

N

G

G

Q

I

F

W

S

A

H

E

L

R

W

A

V

V

D

E

M

M

A

S

E

E

R

R

L

Y

G

G

L

A

E

D

V

V

D

R

C

T

L

I

D

E

R

-

E

-

W

-

G

-

T

-

G

G

V

P

P

P

V

D

D

R

L

P

Y

F

T

S

-

L

E

E

R

T

L

L

A

A

-

R

A

A

D

I

K

E

A

L

H

H

R

Q

I

P

K

K

A

C

V

L

F

F

V

L

T

T

H

H

N

G

E

D

T
x
S

A

S

T

S

G

G

V

L

T

L

S

Q

D

P

V

L

A

E

A

G

V

V

R

G

A

Q

A

I

L

-

D

-

A

C

S

H

G

Q

H

H

P

D

A

C

L

L

F

I

V

V

D

D

G

A

V

V

S

A

S

S

I

L

G

C

S

G

I

V

D

P

F

F

R

Y

Q

M

D

D

E

K

W

W

G

E

V

I

D

D

C

A

A

V

V

Y

S

T

G

G

S

A

Q
|
Q

K
|
K

G

V

F

L

M

G

L

A

P

P

A

P

G

G

L

I

G

T

F

P

L

I

S

S

V

I

S

S

Q

P

K

K

A

A

L

L

E

D

A

V

A

I

K

R

T

N

A

R

R

R

H

T

M

K

R

S

C

K

Y

V

F

F

S

Y

F

W

E

D

D

L

M

L

I

L

K

L

T

G

N

N

D

Y

T

W

G

G

Y

C

F

Y

P

D

-

E

-

P

-

K

-

R

-
x
Y

-

H

Y

H

T
|
T

P

V

P

A

T

S

Q

N

L

L

L

I

H

F

G

A

L

L

R

R

A

E

A

A

L

L

D

A

V

Q

I

I

F

A

E

E

G

G

L

L

E

E

N

N

I

Q

F

I

A

K

R

R

H

R

R

I

H

E

L

C

A

A

D

Q

G

I

V

L

R

Y

A

E

A

G

V

L

S

G

A

K

W

M

G

G

L

L

K

D

L

I

C

F

A

V

T

K

E

D

P

P

K

R

W

H

Y

R

S

L

D

P

T

T

V

V

S

T

A

G

I

I

R

M

L

I

P

P

E

K

G

G

I

V

D

D

G

W

V

W

K

K

V

V

I

S

R

Q

H

Y

A

A

F

M

D

N

T

N

Y

F

N

S

T

L

S

E

L

V

G

Q

S

G

G

G

L

L

S

G

K

P

V

T

A

F

G

G

K

K

V

A

F

W

R

R

I

V

G

G

H

I

L

M

G

G

S

E

L

C

N

S

E

T

V

V

SAH 77:79 (≠ SGT 63:65) binding in other chain
S154 (≠ T143) binding in other chain
Q204 (= Q195) binding in other chain
K205 (= K196) modified: N6-(pyridoxal phosphate)lysine
Y256 (vs. gap) binding pyridoxal 5'-phosphate
T259 (= T244) binding pyridoxal 5'-phosphate

Query Sequence

>WP_012709136.1 NCBI__GCF_000018545.1:WP_012709136.1
MSGYNHLFIPGPTNIPEQIRQAMNLPMEDMRSPRYPELTLPLFADVKKVFKNRNGRVFIY
PSSGTGAWEAAMTNVLSPGDRVLMSRFGQFSHLWVDMAERLGLEVDCLDREWGTGVPVDL
YTERLAADKAHRIKAVFVTHNETATGVTSDVAAVRAALDASGHPALLFVDGVSSIGSIDF
RQDEWGVDCAVSGSQKGFMLPAGLGFLSVSQKALEAAKTARHMRCYFSFEDMIKTNDTGY
FPYTPPTQLLHGLRAALDVIFEEGLENIFARHRHLADGVRAAVSAWGLKLCATEPKWYSD
TVSAIRLPEGIDGVKVIRHAFDTYNTSLGSGLSKVAGKVFRIGHLGSLNEVMVLGALSAA
ELTLLDCGVKIEPGAGVGAAIRQFRAAATATAKAA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory